Multi-channel sliding microfluidic immunoassay chip

Abstract

The application relates to the microfluidic technology field and provides a multi-channel sliding microfluidic immune detection chip, a sample inlet part and at least two detection units are respectively arranged on the chip body, a sliding structure is further arranged on the chip body, a gas outlet and a sample storage channel are further respectively arranged on the chip body, the sample storage channel comprises at least two sample storage branches which are uniformly arranged at intervals, a matching part and at least two air inlets are respectively arranged on the sliding structure, the matching part is used for connecting the at least two sample storage branches to form a first connecting path, and the sample inlet part, the first connecting path and the gas outlet are sequentially connected. The application has the effects of providing quantitative delivery and improving detection accuracy.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of microfluidic technology, in particular to a multi-channel sliding microfluidic immunoassay chip. BACKGROUND

[0002] Microfluidic technology can integrate sample preparation, reaction, separation and other basic operation units in the biochemical analysis process on a chip to automatically complete the analysis process, which has the advantages of less sample consumption, fast detection speed and simple operation, and is often used in marker detection in the medical field to diagnose related diseases.

[0003] The existing microfluidic detection chip adopts a single-piece or multi-channel mode. When a single piece is used for detection, the detection efficiency is low, and the sample is wasted. The multi-channel mode is the mainstream detection mode. The main process is that the sample to be tested enters the chip from the sample inlet, flows along the microchannel inlet in the length direction of the main flow channel under the guidance of the driving piece, and is divided into each microchannel under the driving action of the driving piece and flows towards the waste pool. When flowing through the detection unit, the sample to be tested in the sample to be tested reacts with the substances in the detection unit, and then is scanned and detected by an external scanner. After detection, it flows into the waste pool for treatment.

[0004] However, in the multi-channel mode, the sample flow is directly and completely flowed into each microchannel, and the flow volume is difficult to accurately control, making it difficult to determine the delivery amount and reducing the detection accuracy. SUMMARY

[0005] In order to improve the problem that the flow volume is difficult to accurately control and difficult to determine the delivery amount, the present application provides a multi-channel sliding microfluidic immunoassay chip.

[0006] The multi-channel sliding microfluidic immunoassay chip provided by the present application adopts the following technical scheme:

[0007] A multi-channel sliding microfluidic immunoassay chip, the chip body is respectively provided with a sample inlet piece and at least two detection units;

[0008] Further comprising a sliding structure, the chip body is further respectively provided with an air outlet and a sample storage channel, the sample storage channel comprises at least two sample storage branches which are arranged at intervals and have the same size, the sliding structure is respectively provided with a matching piece and at least two air inlet pieces, the matching piece is used to connect the at least two sample storage branches to form a first communication path, and the sample inlet piece, the first communication path and the air outlet are sequentially connected;

[0009] Each of the air inlets is arranged at a preset distance from the liquid inlet of the detection unit, and the sliding structure is slidably arranged on the chip body along a first direction, so that after the sliding structure is slid by a preset distance along the first direction, each of the air inlets is in communication with the liquid inlet of the detection unit through the corresponding cooperating part and the corresponding sample storage branch.

[0010] By adopting the above technical solution, after the cooperating part on the sliding structure forms the first communication path, the external driving part draws air from the air outlet, so that the liquid sample of the sample inlet is temporarily stored in the sample storage branch entering the first communication path. The sliding structure is manually slid by a preset distance along the first direction, so that the air inlet is in communication with the corresponding sample storage branch, the cooperating part connects the corresponding sample storage branch and the liquid inlet of the detection unit, and the external driving part draws air from the air outlet to form negative pressure, so that the liquid sample in the sample storage branch flows into the detection unit through the cooperating part for detection. By realizing the flowing of the liquid into the sample storage branch with consistent size, when the sample storage branch is full, the liquid sample in the sample storage branch is transported and distributed one by one, so as to achieve the effect of quantitative and equal transportation.

[0011] Optionally, the cooperating part includes at least two first communication parts and at least two second communication parts; the at least two first communication parts are used to connect the at least two sample storage branches to form the first communication path; after the cooperating part slides by a preset distance along the first direction, the second communication part is in communication with the corresponding sample storage branch to form a second communication path, and the corresponding air inlet, the corresponding second communication path and the corresponding detection unit are sequentially connected.

[0012] By adopting the above technical solution, after all the first communication parts, the sample inlet, all the sample storage branches and the air outlet are connected, under the extraction of the external driving part, the liquid sample of the sample inlet can enter the sample storage branch through the first communication part, after the sliding structure is slid by a preset distance along the first direction, the external driving part draws air from the air outlet, and the liquid sample in the sample storage branch enters the detection unit through the second communication part, so that the detection unit can detect the liquid sample.

[0013] Optionally, the sensor is further included, and the sensor is arranged at least three, and the sensor is arranged one by one corresponding to the end of the sample inlet close to the air outlet and the detection end of the at least two detection units.

[0014] By adopting the above technical solution, one of the sensors is installed on the side of the sample inlet close to the air outlet, for detecting whether the sample storage channel is full of liquid sample, and the other sensors are sequentially installed at the end of the detection unit, for detecting the amount of liquid sample entering the detection unit, and can send an electric signal to control the external driving part to stop running.

[0015] Optionally, the chip body is provided with a waste pool, which is communicated with the liquid outlet and the gas outlet of the at least two detection units.

[0016] By adopting the above technical scheme, the waste liquid pool is arranged at the liquid outlet position of the detection unit, so that the liquid sample after detection can be recycled and uniformly treated, and the waste liquid pool is connected with the gas outlet, so that the external driving element extracts the air in the waste liquid pool, and the liquid sample in the detection unit can flow.

[0017] Optionally, the chip body is provided with a sliding groove matched with the sliding structure.

[0018] By adopting the above technical scheme, the sliding groove can facilitate stable sliding of the sliding structure, provide limiting effect, and avoid deviation of the sliding structure during sliding, so as to affect the accuracy of liquid sample transmission.

[0019] Optionally, the sliding groove is arranged along the length direction of the chip body in the first direction, and has a special-shaped groove shape.

[0020] By adopting the above technical scheme, the special-shaped groove can limit the travel trajectory of the sliding structure, avoid deviation, lock the sliding structure, and make the sliding structure only move through the sliding groove and be pulled away from the sliding groove.

[0021] Optionally, the shape and size of the air inlet member and the matching member are the same, the shape and size of the sample inlet member, the storage channel and the liquid inlet of the detection unit are the same, and the shape and size of the air inlet member and the matching member are smaller than the shape and size of the sample inlet member, the storage channel and the liquid inlet of the detection unit.

[0022] By adopting the above technical scheme, the shape and size of the air inlet member and the matching member are smaller than the shape and size of the sample inlet member, the storage channel and the liquid inlet of the detection unit, the air inlet member and the matching member can be inserted and connected with the corresponding sample inlet member, storage channel and liquid inlet of the detection unit, so that the liquid sample is difficult to leak out, and the stability of conveying is improved.

[0023] Optionally, the air inlet member and the matching member further comprise a sealing member close to one end of the detection unit, and the shape and size of the sealing member are larger than the shape and size of the sample inlet member, the storage channel and the liquid inlet of the detection unit.

[0024] By adopting the above technical scheme, the sealing member can further block, further avoid liquid sample leakage, and further ensure the stability of conveying and the accuracy of detection.

[0025] Optionally, it further comprises a clamping member, one end of the clamping member is installed on the chip body, and the other end is connected with the sliding structure.

[0026] By adopting the technical scheme, when it is necessary to fix the sliding structure to avoid loosening of the sliding structure, the sliding structure is pressed by the clamping piece to ensure stable installation of the sliding structure and the sealing piece.

[0027] Optionally, the chip body is further provided with a cleaning structure, which is communicated with the sample inlet.

[0028] By adopting the technical scheme, when the external driving piece is used to draw air, only the cleaning liquid in the cleaning structure can be drawn, and the positions of all the cooperating pieces are simultaneously switched by the sliding assembly, the starting and stopping of the external driving piece are controlled by the sensor, the cleaning liquid is simultaneously circulated into all the channels of the chip body along with the sliding assembly, and finally, the cleaning liquid is simultaneously flowed into the waste liquid pool for unified treatment.

[0029] In summary, the present application has at least one of the following beneficial technical effects:

[0030] 1. After the cooperating piece on the sliding structure forms the first communication path, the external driving piece is used to draw air from the air outlet, so that the liquid sample of the sample inlet is temporarily stored in the sample storage branch in the first communication path. The sliding structure is manually slid in the first direction by a preset distance, so that the air inlet is communicated with the corresponding sample storage branch. The cooperating piece communicates the corresponding sample storage branch with the liquid inlet of the detection unit. The external driving piece draws air from the air outlet to form negative pressure, so that the liquid sample in the sample storage branch flows into the detection unit through the cooperating piece for detection. By flowing the liquid into the sample storage branch with the same size, the liquid sample in the sample storage branch is transported and distributed one by one after the sample storage branch is full, so as to achieve the effect of quantitative and equal transportation.

[0031] 2. The shape and size of the air inlet and the cooperating piece are smaller than those of the sample inlet, the storage channel and the liquid inlet of the detection unit. The air inlet and the cooperating piece can be inserted into the corresponding sample inlet, storage channel and liquid inlet of the detection unit, so that the liquid sample is difficult to leak out, and the stability of transportation is improved.

[0032] 3. The sliding structure can change the insertion positions of the cooperating piece, the air inlet and the access channel by moving along the special-shaped groove. After switching the insertion positions of the cooperating piece, the air inlet and the access channel in the chip body, the cooperating piece, the air inlet and the access channel are fixed by the clamping piece, so as to press the sealing pieces on the cooperating piece, the air inlet and the access channel, improve the sealing effect, avoid the leakage of the liquid sample, and affect the detection result.

[0033] 4. The sample inlet is covered by the external closure cover. When the external driving piece draws air, only the cleaning liquid in the cleaning structure can be drawn. The positions of all the cooperating pieces are simultaneously switched by the sliding assembly. The starting and stopping of the external driving piece are controlled by the sensor. The cleaning liquid is simultaneously circulated into all the channels of the chip body along with the sliding assembly. Finally, the cleaning liquid is simultaneously flowed into the waste liquid pool for unified treatment. BRIEF DESCRIPTION OF DRAWINGS

[0034] Figure 1 is a first cross-sectional structure schematic diagram of a first state of a chip in Embodiment 1 of the present application;

[0035] Figure 2 is a first cross-sectional structure schematic diagram of a second state of the chip in Embodiment 1 of the present application;

[0036] Figure 3 is a second cross-sectional structure schematic diagram of a second state of a chip in Embodiment 2 of the present application;

[0037] Figure 4 is an enlarged schematic diagram of A in the present application; Figure 3

[0038] Figure 5 is a third cross-sectional structure schematic diagram of a second state of a chip in Embodiment 2 of the present application;

[0039] Figure 6 is an enlarged schematic diagram of B in the present application; Figure 5

[0040] Figure 7 is a three-dimensional structure schematic diagram of a chip in Embodiment 2 of the present application;

[0041] Figure 8 is a fourth cross-sectional structure schematic diagram of a second state of a chip in Embodiment 3 of the present application;

[0042] The marks in the drawings are as follows: 1, chip main body, 11, sample injection piece, 12, detection unit, 121, reaction pool, 122, scanning pool, 123, reaction piece, 13, storage channel, 14, gas outlet, 15, waste liquid pool, 16, sliding groove, 2, sliding structure, 21, matching piece, 211, first communication part, 212, second communication part, 22, gas injection piece, 23, sealing piece, 3, sensor, 4, clamping piece, 5, cleaning structure. DETAILED DESCRIPTION

[0043] The present application will be further described in detail below with reference to the accompanying drawings. Figure 1 - the accompanying drawings, Figure 8 .

[0044] The embodiments of the present application disclose a multi-channel sliding microfluidic immunodetection chip.

[0045] Embodiment 1:

[0046] A multi-channel sliding microfluidic immunodetection chip, referring to Figure 1 and Figure 2 ​​As shown, including the chip body, the chip body is respectively provided with sample injection member 11 and at least two detection units 12. Specifically, the chip main body 1 is the main bearing for detection structure installation and detection, the sample injection member 11 is provided with a sample port for sample entering, the sample port can be sealed by a sealing cover, the detection unit 12 provides a reaction and detection place for liquid sample, the number of detection units 12 is determined according to actual needs, and three detection units 12 are provided in the embodiment.

[0047] Further comprising sliding structure 2, the chip body is further respectively provided with gas outlet 14 and sample storage channel, the sample storage channel comprises at least two spaced sample storage branches, the sliding structure 2 is respectively provided with cooperating member 21 and at least two air inlet members 22, the cooperating member 21 is used for connecting the at least two sample storage branches to form a first communication path, the sample injection member 11, the first communication path and the gas outlet 14 are sequentially communicated. The gas outlet 14 is connected with a driving member, which is used to drive the liquid sample to flow, and any power source that can form negative pressure such as air pump or piston syringe can be used. The number of air inlets and sample storage branches is consistent with the number of detection units 12, and three are provided in the embodiment. After the cooperating member 21 on the sliding structure 2 forms the first communication path, the gas outlet 14 is pumped by the external driving member, so that the liquid sample of the sample injection member 11 enters the sample storage branch in the first communication path. The sample storage branches are of the same size, so the liquid sample is temporarily stored after entering the sample storage branch. The sliding structure 2 is manually slid in the first direction by a preset distance, the first direction can be the direction of the sample port of the sample injection member 11, and the preset distance can be set according to the size of the specific chip main body 1. After the sliding structure 2 slides by the preset distance, the air inlet is communicated with the corresponding sample storage branch, the cooperating member 21 connects the corresponding sample storage branch with the liquid inlet of the detection unit 12, the external driving member pumps the gas outlet 14 to form negative pressure, so that the liquid sample in the sample storage branch flows into the detection unit 12 through the cooperating member 21 for detection. By realizing that the liquid flows into the sample storage branch, the liquid sample in the sample storage branch is transported and distributed one by one, so as to achieve the effect of quantitative and equal transportation.

[0048] The fitting part 21 comprises at least two first communication parts 211 and at least two second communication parts 212; the at least two first communication parts 211 are used to communicate the at least two sample storage branches to form a first communication path; after the fitting part 21 slides in the first direction by a preset distance, the at least two second communication parts 212 respectively communicate with the at least two sample storage branches arranged at intervals to form a second communication path, which is communicated with the corresponding detection unit 12 in sequence corresponding to the air inlet part 22 and the corresponding second communication path; the first communication part 211 is used to communicate the sample storage branches to form the first communication path, and all the first communication parts 211, the sample inlet part 11, all the sample storage branches and the air outlet 14 are communicated with each other, so that the liquid sample of the sample inlet part 11 can be introduced into the sample storage branch through the first communication part 211 under the extraction of the external driving part, and after the sliding structure 2 slides in the first direction by a preset distance, the liquid sample in the sample storage branch enters the detection unit 12 through the second communication part 212, so that the detection unit 12 can detect the liquid sample.

[0049] The sensor 3 is further included, and the sensor 3 is arranged in one-to-one correspondence with the sample inlet part 11 close to one end of the air outlet 14 and the detection ends of the at least two detection units 12; since the number of the detection units 12 in the embodiment is 3, one additional sensor 3 is needed to detect the sample inlet part 11, so the number of the sensor 3 in the embodiment is 4; one of the sensor 3 is installed on the side of the sample inlet part 11 close to the air outlet 14 and is located after all the fitting parts 21 and the sample storage channels, and is used to detect whether the sample storage channels are full of liquid sample; when the liquid sample is detected to be close to entering the air outlet 14, an electric signal is sent to the external driving part, and the external driving part stops air extraction; the other three sensors 3 are sequentially installed at the ends of the detection units 12, that is, the positions of the detection units 12 closest to the liquid outlets, and are used to detect the amount of liquid sample entering the detection units 12; when the liquid sample is detected to be close to entering the liquid outlet, an electric signal is sent to the external driving part, and the external driving part stops air extraction, so as to provide the liquid sample in the detection unit 12 for reaction and scanning.

[0050] The chip main body 1 is provided with a waste pool, and the waste pool is communicated with the liquid outlets of the at least two detection units 12 and the air outlet 14; the waste liquid pool 15 is arranged at the position of the liquid outlet of the detection unit 12, so that the liquid sample after detection can be recycled and uniformly treated, and the air outlet 14 is connected with the waste liquid pool 15, so that the external driving part extracts the air in the waste liquid pool 15, so that the liquid sample in the detection unit 12 can flow.

[0051] The chip main body 1 is provided with a sliding groove 16 for limiting the sliding of the sliding structure 2; the sliding groove 16 can facilitate the stable sliding of the sliding structure 2 and provide a limiting effect, so as to avoid deviation of the sliding structure 2 during sliding and affect the accuracy of liquid sample transmission.

[0052] The detection unit 12 comprises a reaction pool 121, a scanning pool 122 and a reaction member 123; the reaction member 123 sequentially connects the reaction pool 121, the scanning pool 122 and a waste pool, and the scanning pool 122 is connected with a sensing end of an external sensor 3; a driving member is used to form negative pressure in the waste liquid pool 15; chemical substances, such as freeze-dried reagents, exist in the reaction pool 121; the reaction member 123 can adopt a serpentine channel (S type) or a U-shaped channel, and in the embodiment, the serpentine channel is adopted; after the liquid sample enters the reaction member 123 under the negative pressure state through a connecting member, the liquid sample melts the freeze-dried reagents in the reaction pool 121 and mixes and reacts in the serpentine channel; after the mixing and reaction are completed, the sample enters the scanning pool 122; after the liquid sample enters the scanning pool 122, the external sensor 3 stops the driving member and starts to count the incubation time; after the counting is completed, the external scanning instrument scans the scanning pool 122 to obtain the detection result; and finally, under the driving of the driving member, the liquid sample enters the waste pool to be recycled.

[0053] The sample inlet member 11, the reaction member of the detection unit 12 and the storage channel 13 can all adopt the form of a pipeline or a cavity; when the chip main body 1 is solid, the cavity can be adopted; and when the chip main body 1 is hollow, the pipeline can be adopted.

[0054] The implementation principle of the embodiment 1 of the application comprises the following steps: after the matching member 21 on the sliding structure 2 forms a first communication path, the liquid sample of the sample inlet member 11 is temporarily stored in a sample storage branch in the first communication path by means of air extraction of an external driving member on the air outlet 14; the sliding structure 2 is manually slid by a preset distance along a first direction, so that the air inlet is communicated with the corresponding sample storage branch; the matching member 21 communicates the corresponding sample storage branch with the liquid inlet of the detection unit 12; the liquid sample in the sample storage branch flows into the detection unit 12 for detection by means of air extraction of the external driving member on the air outlet 14 to form negative pressure; by means of the implementation that the liquid sample flows into the sample storage branch with the same size, the sample storage branch is filled, and then the liquid sample in the sample storage branch is one-by-one transported and distributed, so that the quantitative and equal amount transportation is achieved.

[0055] Embodiment 2

[0056] Reference Figure 3 and Figure 4As shown, the embodiment 2 of the present application is optimized on the basis of the embodiment 1, including the following optimizations: the shape and size of the sliding air member 22 and the matching member 21 are the same, the shape and size of the sample injection member 11, the storage channel 13 and the liquid inlet of the detection unit 12 are the same, and the shape and size of the sliding air member 22 and the matching member 21 are smaller than those of the sample injection member 11, the storage channel 13 and the liquid inlet of the detection unit 12; specifically, the matching member 21 and the sliding air member 22 adopt a pipeline, the reaction channel of the detection unit 12 and the storage channel 13 can adopt a cavity or a pipeline, the sliding groove 16 is arranged along the length direction of the first direction of the chip main body 1, and has a special-shaped groove shape; the sliding groove 16 can limit the travel trajectory of the sliding structure 2 to avoid deviation, and the special-shaped groove can lock the sliding structure 2, so that the sliding structure 2 can only move through the sliding groove 16, and be pulled away from the sliding groove 16 under stress. The special-shaped groove can adopt a I-shaped groove, a cross-shaped groove, an inverted L-shaped groove or an H-shaped groove, and the embodiment adopts an H-shaped groove, and the sliding structure 2 can move along the H-shaped groove.

[0057] When it is necessary to fill each sample storage branch of the storage channel 13, the sliding structure 2 is horizontally moved to the end of the H-shaped groove, and can continue to vertically move the sliding structure 2, at this time, one end of the matching member 21 is aligned with the sample injection member 11, and the other end is aligned with the storage channel 13. Since the shape and size of the sliding air member 22 and the matching member 21 are smaller than those of the sample injection member 11, the storage channel 13 and the liquid inlet of the detection unit 12, the sliding structure 2 continues to slide in the vertical direction, so that the first connecting part 211 can be inserted into the sample injection member 11, the storage channel 13 and the negative pressure channel, and the first connecting part 211, the sample injection member 11 and the storage channel 13 are connected in series, thereby avoiding the leakage of the liquid sample from the interface when the liquid sample is extracted.

[0058] When the liquid sample in the storage channel 13 is full, the sliding structure 2 slides vertically along the sliding groove 16 to the horizontal direction of the sliding groove 16, allowing the mating part 21 to be pulled out from the sample inlet 11 and each sample storage branch of the storage channel 13. Then, it moves horizontally along the H-shaped groove to the end, which can continue to drive the sliding structure 2 to move vertically. At this time, each air inlet 22 is aligned with each storage branch, one end of the second connecting part 212 is aligned with the outlet of the corresponding storage branch, and the other end is aligned with the liquid inlet of the detection unit 12, i.e., the inlet of the reaction element 123. The sliding structure 2 continues to slide vertically, and the corresponding air inlet 22 is inserted into the inlet of the corresponding storage branch, and the corresponding second connecting part... One end of 212 is inserted into the outlet of the corresponding storage branch, and the other end is inserted into the inlet of the detection unit 12, which is the inlet of the reaction channel. Therefore, the external drive unit draws air from the outlet 14 and forms a negative pressure inside the detection unit 12 through the waste liquid pool 15, so that the liquid samples in each storage branch are drawn into the detection unit 12 for detection. Through the insertion connection, the liquid sample is difficult to leak out, which improves the stability of the transport. When extracting liquid samples from the storage branch, the air inlet that does not need to be detected can be blocked, so that the detection unit 12 that does not need to be detected does not form a passage, and the liquid sample in the corresponding storage branch that does not need to be detected will not flow into the detection unit 12.

[0059] Reference Figure 5 and Figure 6 As shown, the air inlet 22 and the mating part 21 near the detection unit 12 also include a sealing part 23, and the shape and size of the sealing part 23 are larger than the shape and size of the sample inlet 11, the storage channel 13 and the liquid inlet of the detection unit 12; the sealing part 23 can be a sealing ring. The insertion connection end of the air inlet 22, the mating part 21 and the access channel is equipped with a sealing part 23 to further seal, further prevent liquid sample leakage, and further ensure the stability of delivery and the accuracy of detection.

[0060] Reference Figure 7 As shown, it also includes a clamping member 4; one end of the clamping member 4 is installed on the chip body 1, and the other end is connected to the sliding structure 2; the clamping member 4 may include a telescopic rod, an elastic element, and a pressure plate. The telescopic rod is installed on the chip body 1, and relative to the sliding structure 2, the telescopic rod is located on the rear side of the chip body 1. The elastic element is installed inside the telescopic rod to provide the extension elasticity of the telescopic rod. The pressure plate is installed on the extended end of the telescopic rod, and the pressure plate is movably connected to the telescopic rod and can rotate along the extended end of the telescopic rod. When it is necessary to fix the sliding structure 2 and prevent the sliding structure 2 from loosening, the pressure plate is pulled, the pressure plate is stretched and affected by the elastic force of the elastic element, pressing the sliding structure 2 to ensure the stable installation of the sliding structure 2 and the sealing member 23.

[0061] Both the fitting component 21 and the air inlet component 22 are pipes, and the sample injection component 11, the reaction component 123 of the detection unit 12, and the storage channel 13 are all cavities.

[0062] The implementation principle of the embodiment 2 of the present application includes that the sliding structure 2 can change the insertion positions of the matching pieces 21 and the air inlet pieces 22 by moving along the special-shaped grooves, and after switching the various channels on the chip body 1, the matching pieces 21, the air inlet pieces 22 and the sealing pieces 23 on the access channels are pressed tightly by the clamping pieces 4, so as to improve the sealing effect, avoid the leakage of liquid samples and affect the detection results.

[0063] Embodiment 3

[0064] Referring to Figure 8 The embodiment 3 of the present application is optimized on the basis of the embodiment 1 or the embodiment 2, and includes the following optimization, that is, the chip body 1 is further provided with a cleaning structure 5. Specifically, the cleaning structure 5 is installed on the chip body 1 and communicates with the sample inlet piece 11, and the cleaning structure 5 can be a rubber bag or a rubber cover containing cleaning liquid. When the detection is completed, it is very inconvenient to clean the various channels in the chip body 1, so the cleaning structure 5 is arranged to assist in cleaning the various channels of the chip body 1 and the sliding assembly. The main steps are similar to those of the embodiment 1 and the embodiment 2. The sample inlet piece 11 is covered with an external closing cover, and when the external driving piece is pumping, only the cleaning liquid in the cleaning structure 5 can be pumped, and the position of the matching piece 21 is switched by the sliding assembly, and the starting and stopping of the external driving piece is controlled by the sensor 3, so that the cleaning liquid flows to the sample storage channels of the chip body 1, the detection unit 12 and finally to the waste liquid pool 15 for unified treatment.

[0065] The implementation principle of the embodiment 3 of the present application includes that the sample inlet piece 11 is covered with an external closing cover, and when the external driving piece is pumping, only the cleaning liquid in the cleaning structure 5 can be pumped, and the positions of all the matching pieces 21 are switched by the sliding assembly at the same time, and the starting and stopping of the external driving piece is controlled by the sensor 3, so that the cleaning liquid flows to the various channels of the chip body 1 at the same time, and finally flows to the detection unit 12 for unified treatment.

[0066] The embodiments of the present application are preferred embodiments of the present application, and are not limited to the protection scope of the present application. The same parts are indicated by the same reference numerals. Therefore, equivalent changes made according to the structure, shape and principle of the present application should be covered by the protection scope of the present application.

Claims

1. A multi-channel sliding microfluidic immunoassay chip, characterized in that, Including chip body (1), the chip body (1) is equipped with sample inlet piece (11) and at least two detection units (12) respectively; It also includes sliding structure (2), the chip body (1) is also equipped with gas outlet (14) and sample storage channel respectively, the sample storage channel includes at least two interval arrangement size consistent sample storage branch, the sliding structure (2) is equipped with cooperation piece (21) and at least two air inlet piece (22) respectively, the cooperation piece (21) is used to communicate at least two sample storage branches to form first communication path, the sample inlet piece (11), first communication path and the gas outlet (14) are sequentially communicated; Each air inlet piece (22) is correspondingly arranged with liquid inlet in the detection unit (12), and is spaced apart by a preset distance, the sliding structure (2) is slidably arranged on the chip body (1) along the first direction, so that after the sliding structure (2) slides by a preset distance along the first direction, each air inlet piece (22) is communicated with the corresponding liquid inlet in the detection unit (12) through the corresponding cooperation piece (21) and the corresponding sample storage branch one by one;The cooperation piece (21) includes at least two first communication parts (211) and at least two second communication parts (212);At least two first communication parts (211) are used to communicate at least two sample storage branches to form first communication path;After the cooperation piece (21) slides by a preset distance along the first direction, the second communication part (212) is communicated with the corresponding sample storage branch to form second communication path, and the corresponding air inlet piece (22), the corresponding second communication path and the corresponding detection unit (12) are sequentially communicated;It also includes sensor (3), the sensor (3) is at least three, the sensor (3) is arranged one by one with the sample inlet piece (11) close to the one end of the gas outlet (14) and the detection end of at least two detection units (12);The chip body (1) is provided with a waste pool, and the waste pool is communicated with the liquid outlet of at least two detection units (12) and the gas outlet (14) respectively.

2. The multi-channel sliding microfluidic immunoassay chip according to claim 1, wherein, The chip body (1) is provided with a sliding groove (16) for limiting the sliding of the sliding structure (2).

3. The multi-channel sliding microfluidic immunoassay chip according to claim 2, wherein, The sliding groove (16) is arranged along the length direction of the chip body (1) in the first direction, and is shaped as a special-shaped groove.

4. The multi-channel sliding microfluidic immunoassay chip according to claim 1, wherein, The shape and size of the air inlet piece (22) and the cooperation piece (21) are the same, the shape and size of the sample inlet piece (11), the storage channel (13) and the liquid inlet of the detection unit (12) are the same, and the shape and size of the air inlet piece (22) and the cooperation piece (21) are smaller than the shape and size of the sample inlet piece (11), the storage channel (13) and the liquid inlet of the detection unit (12).

5. The multi-channel sliding microfluidic immunoassay chip according to claim 4, wherein, The end of the air inlet piece (22) and the cooperation piece (21) close to the detection unit (12) further includes a sealing piece (23), and the shape and size of the sealing piece (23) are larger than the shape and size of the sample inlet piece (11), the storage channel (13) and the liquid inlet of the detection unit (12).

6. The multi-channel sliding microfluidic immunoassay chip according to claim 3, wherein, Also include clamping piece (4), one end of clamping piece (4) is installed on the chip body (1), the other end is connected with sliding structure (2).

7. The multi-channel sliding microfluidic immunoassay chip according to claim 1, wherein, The chip body (1) is also provided with a cleaning structure (5) which is communicated with the sample feeding member (11).

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