Fluorescence detection microfluidic chip, preparation method thereof and fluorescence detection system
By using polytetrafluoroethylene (PTFE) to fabricate microfluidic chips and utilizing its low refractive index to form liquid optical waveguides, the problem of material background fluorescence noise was solved, achieving high-sensitivity fluorescence detection and constructing an ultrasensitive fluorescence detection system.
CN116212986BActive Publication Date: 2026-06-19NAT INST OF ADVANCED MEDICAL DEVICES SHENZHEN
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- NAT INST OF ADVANCED MEDICAL DEVICES SHENZHEN
- Filing Date
- 2022-12-07
- Publication Date
- 2026-06-19
Smart Images
Figure CN116212986B_ABST
Abstract
This invention discloses a fluorescence detection microfluidic chip and its fabrication method. The fluorescence detection microfluidic chip includes a first film layer and a second film layer. The first film layer includes a main layer and multiple elongated protrusions. The main layer, two adjacent elongated protrusions, and the second film layer together form a microchannel. The main layer, the elongated protrusions, and the second film layer are all made of polytetrafluoroethylene (PTFE). In the fluorescence detection microfluidic chip of this invention, the refractive index of PTFE (1.29) is lower than that of water (1.33), thus allowing the relatively high refractive index of the sample to be tested to form a liquid optical waveguide, confining the light field within the microchannel. This effectively delivers the sample while maximizing the interaction between light and the analyte, thereby effectively reducing the background fluorescence noise generated by the material itself and improving detection sensitivity. It can be used to construct a high-sensitivity fluorescence detection system.
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