A method for planting oil-tea-pollinating trees

Abstract

The invention discloses a method for planting oil-tea pollination trees in the field of plant pollination technology. The method comprises the following steps: S1. selecting four Changlin oil-tea series to carry out mixed material experiments by artificial pollination and natural pollination to design 20 pollination combinations, and counting the number, quality and fruit setting rate of oil-tea fruits in different combinations; S2. calculating the fruit setting rate of artificial pollination in the whole flowering period A=(Z×Y) / X, and obtaining the actual oil yield of a single oil-tea plant in the whole flowering period artificial pollination; S3. calculating the oil yield of a single oil-tea plant under different mixed material ratios by mixed material experiment design, inputting the data into JMP software, and predicting the optimal variety configuration; S4. making a linear fitting equation for the influence of different mixed material ratio combinations on the oil-tea yield by Origin software; S5. predicting the trend of the influence of four oil-tea varieties on the oil yield by a prediction profiler, and calculating the optimal planting scheme for oil-tea pollination trees.

Description

Technical Field

[0001] The invention belongs to the technical field of plant pollination, in particular to a method for planting oil-tea pollination trees. Background Art

[0002] Camellia oleifera belongs to the Theaceae family. It is also known as tea tree, tea oil tree, and white flower tea. It is a high-quality woody oil plant unique to the hilly and mountainous areas in southern my country. It is also one of the most important woody oil tree species in the world. It is known as the world's four major woody oil plants together with woody oil plants such as olive, oil palm, and coconut. It has a long history of cultivation and consumption. By the end of 2020, there were more than 68 million mu of oil tea forests in China, distributed in 14 provinces and regions. Camellia oil is a high-quality edible oil that contains more than 80% of the unsaturated fatty acids needed by the human body. It has the effects of lowering blood pressure, blood lipids, and softening blood vessels. It enjoys the reputation of "Oriental Olive Oil". Some genetic characteristics of economic forest tree species are not conducive to fruit setting and fruit development. For example, some tree species or varieties are self-pollinating or have low self-pollination rates, some tree species are dioecious, some varieties have stamen abortion, and some varieties have extremely different fruit setting rates between cross-pollination and reverse cross-pollination. Therefore, the correct selection and configuration of pollination trees, overcoming these genetic characteristics that are not conducive to flowering and fruiting, and improving the fruit setting rate are key measures for high yield, stable yield and high-quality fruit of economic forest tree species. Oil tea blooms in winter, has poor self-affinity, has more flowers and fewer fruits, and has a low fruit setting rate, which is one of the fundamental reasons for low yield. Therefore, it is an important scientific research topic in the oil tea industry to select reasonable varieties for configuration planting and improve oil tea yield and fruit quality. At the same time, the study of pollination tree configuration is of great significance to fruit trees whose main harvesting purpose is planting fruits and the economic forest industry. It can provide a theoretical basis for the selection of pollination trees, the increase of yield, and the improvement of internal and external quality in the actual production process.

[0003] Chinese patent publication number CN111837786A proposes a cultivation method for multiple varieties of oil-tea camellia, and the steps are as follows: select mature oil-tea camellia fruits with good quality and screen out suitable seeds; build a nursery bed on the nursery site, and the nursery bed is padded with gravel or coarse sand; pre-treat the nursery substrate and put it into a non-woven seedling container, and place it in the nursery bed; disinfect the nursery substrate one day before nurserying; punch holes in the non-woven seedling container for sowing, and water thoroughly after sowing; manage the nursery process according to conventional methods; select a suitable afforestation site for planting, remove weeds and dig planting holes, set up windbreaks, work roads and soil and water conservation projects; select normal and healthy seedlings for transplanting and planting, and water each tree thoroughly after planting; carry out management in terms of shaping, pruning, weeding, fertilizing, disease and pest control, etc.; collect pollen from different varieties of oil-tea camellia and mix them, and spray them with a sprayer or drone during the flowering period.

[0004] However, the above technical scheme does not take into account the significant pollen xenia effect of oil tea and the different fruit setting rates among different varieties. Therefore, it is necessary to propose a method for planting oil tea pollination trees to obtain the best yield by finding the most suitable ratio of pollination trees for different varieties through artificial self-pollination, cross-pollination and natural pollination experiments. Summary of the invention

[0005] The purpose of the present invention is to provide a method for planting oil-tea pollination trees that significantly improves the yield of oil-tea. This method is not only applicable to other varieties of oil-tea, but also to economic forest tree species that are self-pollinating infertile or have poor self-pollinating yields, but whose yields can be greatly improved by cross-pollination.

[0006] In order to achieve the above object, the technical solution of the present invention is as follows: A method for planting oil-tea pollination trees, comprising the following steps:

[0007] S1. Select Changlin Camellia oleifera series as the test materials;

[0008] S2. The oil-tea camellia varieties were mixed and designed into 20 pollination ratio combinations by artificial self-pollination, outcrossing pollination and natural pollination, and the relevant data of oil-tea camellia fruits and seeds in different combinations were statistically analyzed, including the quantity, quality, oil content and fruit setting rate of fruits and seeds;

[0009] S3. Statistical calculation of natural pollination fruiting rate X, natural pollination fruiting rate Y, artificial pollination fruiting rate Z, calculated the artificial pollination fruiting rate A = (Z × Y) / X, to obtain the actual oil yield of a single tea plant artificial pollination during the entire flowering period;

[0010] S4. The oil yield of a single camellia plant under different mixture ratios was calculated by the mixture test design calculation method, and the oil yield data of a single camellia plant was input into the JMP software. The optimal variety configuration was predicted by the JMP software, and the JMP software was fitted with a predicted value-actual value image;

[0011] S5. Using Origin software, a linear fitting equation was made to show the effect of different mixing ratios on the oil-tea camellia yield. The linear fitting equation can be used to calculate the oil yield of each oil-tea camellia plant within its value range for each variety.

[0012] S6. predicting the influence of the tea varieties on the oil yield of a single tea plant in the mixture ratio test within the value range through the prediction profiler;

[0013] S7. Calculate the optimal pollination mixture ratio combination to obtain the best planting plan for oil tea pollination trees.

[0014] Furthermore, in S1, the varieties of tea oil test materials are Changlin No. 3, Changlin No. 4, Changlin No. 40 and Changlin No. 53, among which the tea oil test materials are selected from plants with good growth conditions and consistent tree height and crown width of the same variety.

[0015] Furthermore, in S2, mature camellia oleifera fruits were collected from the middle and upper part of the outer crown and multiple indices of the camellia oleifera fruits were measured, including the quality, quantity, kernel oil content and fresh fruit oil content of the fruits and seeds; within 24 hours of fruit harvest, the fresh fruit quality and dry seed quality were measured using an electronic balance; the fresh camellia oleifera seeds were placed in a 60°C oven and dried to constant weight, and the dry seed quality and dry kernel quality were weighed; the kernel oil content was measured using the Soxhlet extraction method, and the calculation method was as follows:

[0016] Dry kernel yield = dry kernel mass / dry seed mass × 100% (1)

[0017] Kernel oil content = oil mass / dry kernel mass × 100% (2)

[0018] Fresh fruit oil content = dry seed mass × dry seed kernel rate × kernel oil content / fresh fruit mass × 100% (3)

[0019] Furthermore, in S3, during the flowering period, the buds that are about to open are emasculated during artificial pollination, and according to the complete diallel hybridization test design, pollination and tape sealing are performed, and the number of pollinated flowers and the total number of flowers per plant of each variety are counted. At the same time, the flowers that are naturally pollinated during the flowering period are marked, and the fruit setting of each pollination combination and each variety per plant is counted when the fruit is ripe, and the fruit setting rate is calculated. The calculation formula for the fruit setting rate of oil tea during the entire flowering period of artificial pollination is as follows:

[0020] X = Number of fruits marked at the peak flowering stage / Number of flower buds marked at the peak flowering stage × 100% (4)

[0021] Y = number of natural fruits / number of flower buds × 100% (5)

[0022] Z = Number of pollinated fruits / number of pollinated flowers × 100% (6)

[0023]

[0024] Among them, X is the natural pollination fruit setting rate during the peak flowering period of Camellia oleifera, Y is the natural pollination fruit setting rate during the entire flowering period, and Z is the artificial pollination fruit setting rate during the peak flowering period.

[0025] Furthermore, in S3, the calculation formula for the oil yield of a single oil-tea camellia plant is as follows: Oil yield of a single oil-tea camellia plant = number of flower buds × fruit setting rate × single fruit weight × fresh fruit oil content.

[0026] Furthermore, in S4, the oil yield of a single camellia oleifera plant under different mixture ratios is calculated through a mixture test design calculation method.

[0027] The following beneficial effects are achieved by adopting the above scheme:

[0028] The present invention uses the main cultivated varieties of oil tea 'Changlin No. 3', 'Changlin No. 4', 'Changlin No. 40' and 'Changlin No. 53' as test materials to carry out artificial self-pollination, cross-pollination and natural pollination experiments. Through mixed test design, it is found that there is a significant pollen direct effect in oil tea, and pollination between different varieties can greatly improve the fruit setting rate. Different pollens have a significant impact on the fruit setting rate, the internal and external quality of the fruit, the fatty acid composition of oil and trace nutrients of contemporary hybrids of oil tea. Through statistics, calculation and analysis, the most suitable pollination tree ratio for different varieties is found to obtain the maximum benefit. This method can be used for a variety of excellent oil tea lines, such as "Sanhua series", "Ganwu series", etc. In the large-scale production and planting process of oil tea, a scientific basis is provided for the quantity configuration of different oil tea varieties. At the same time, the maximum yield of oil tea can be predicted to solve the problem of low fruit setting rate of oil tea in production, and promote the efficient and sustainable development of the oil tea industry. At the same time, this method is still applicable to tree species or varieties that do not self-fruit (such as apple, pear, plum, plum, grapefruit) and tree species or varieties that have little self-fruit (such as lychee, longan, loquat, peach, jujube, oil tea, etc.). Through this method, pollination trees can be reasonably selected and configured, promoting the development of fruit trees and economic forest industries whose main harvesting purpose is to grow fruits, and providing a theoretical basis for the selection of pollination trees, the increase in yield, and the improvement of internal and external quality in actual production processes. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 This is a comparison chart of "predicted value - actual value" of the actual oil yield of the mixed material ratio during the whole flowering period of the embodiment of the present invention.

[0030] Figure 2 It is a predictive characterizer for the effect of different tea oil varieties on oil yield in the mixing ratio according to the embodiment of the present invention. DETAILED DESCRIPTION

[0031] The following is further described in detail through specific implementation methods:

[0032] The embodiment is basically as shown in the attached Figure 1 and attached Figure 2 As shown:

[0033] A method for planting oil-tea pollination trees, comprising the following steps:

[0034] S1. Select Changlin Camellia oleifera series as the test materials;

[0035] S2. The oil-tea camellia varieties were mixed and designed into 20 pollination ratio combinations by artificial self-pollination, outcrossing pollination and natural pollination, and the relevant data of oil-tea camellia fruits and seeds in different combinations were statistically analyzed, including the quantity, quality, oil content and fruit setting rate of fruits and seeds;

[0036] S3. Statistical calculation of natural pollination fruiting rate X, natural pollination fruiting rate Y, artificial pollination fruiting rate Z, calculated the artificial pollination fruiting rate A = (Z × Y) / X, to obtain the actual oil yield of a single tea plant artificial pollination during the entire flowering period;

[0037] S4. The oil yield of a single camellia plant under different mixture ratios was calculated by the mixture test design calculation method, and the oil yield data of a single camellia plant was input into the JMP software. The optimal variety configuration was predicted by the JMP software, and the JMP software was fitted with a predicted value-actual value image;

[0038] S5. Using Origin software, a linear fitting equation was made to show the effect of different mixing ratios on the oil-tea camellia yield. The linear fitting equation can be used to calculate the oil yield of each oil-tea camellia plant within its value range for each variety.

[0039] S6. predicting the influence of the tea varieties on the oil yield of a single tea plant in the mixture ratio test within the value range through the prediction profiler;

[0040] S7. Calculate the optimal pollination mixture ratio combination to obtain the best planting plan for oil tea pollination trees.

[0041] In S1, the varieties of tea oil test materials are Changlin No. 3, Changlin No. 4, Changlin No. 40 and Changlin No. 53. Among them, the tea oil test materials are selected from plants with good growth conditions and consistent tree height and crown width of the same variety.

[0042] In S2, mature camellia oleifera fruits were collected from the middle and upper part of the outer crown and multiple indicators of the camellia oleifera fruits were measured, including the quality, quantity, kernel oil content and fresh fruit oil content of the fruits and seeds; within 24 hours of fruit harvest, the fresh fruit quality and dry seed quality were measured using an electronic balance; the fresh camellia oleifera seeds were placed in a 60°C oven and dried to constant weight, and the dry seed quality and dry kernel quality were weighed; the kernel oil content was measured using the Soxhlet extraction method, and the calculation method was as follows:

[0043] Dry kernel yield = dry kernel mass / dry seed mass × 100% (1)

[0044] Kernel oil content = oil mass / dry kernel mass × 100% (2)

[0045] Fresh fruit oil content = dry seed mass × dry seed kernel rate × kernel oil content / fresh fruit mass × 100% (3)

[0046] Furthermore, in S3, during the flowering period, the buds that are about to open are emasculated during artificial pollination, and according to the complete diallel hybridization test design, pollination and tape sealing are performed, and the number of pollinated flowers and the total number of flowers per plant of each variety are counted. At the same time, the flowers that are naturally pollinated during the flowering period are marked, and the fruit setting of each pollination combination and each variety per plant is counted when the fruit is ripe, and the fruit setting rate is calculated. The calculation formula for the fruit setting rate of oil tea during the entire flowering period of artificial pollination is as follows:

[0047] X = Number of fruits marked at the peak flowering stage / Number of flower buds marked at the peak flowering stage × 100% (4)

[0048] Y = number of natural fruits / number of flower buds × 100% (5)

[0049] Z = Number of pollinated fruits / number of pollinated flowers × 100% (6)

[0050]

[0051] Among them, X is the natural pollination fruit setting rate during the peak flowering period of Camellia oleifera, Y is the natural pollination fruit setting rate during the entire flowering period, and Z is the artificial pollination fruit setting rate during the peak flowering period.

[0052] In S3, the calculation formula for the oil yield of a single tea tree is as follows: Oil yield of a single tea tree = number of flower buds × fruit setting rate × single fruit weight × fresh fruit oil content.

[0053] In S4, the oil yield of a single camellia oleifera plant under different mixture ratios is calculated through the mixture test design calculation method.

[0054] The specific implementation process is as follows:

[0055] The test site is located at the Camellia oleifera demonstration base of the Subtropical Forestry Experimental Center of the Chinese Academy of Forestry in Fenyi County, Xinyu City, Jiangxi Province (27°49′19″N, 114°39′13″E), with an average altitude of 115m, an average annual precipitation of 1640.9mm, an average annual sunshine duration of 1543.5h, and an average annual temperature of 17.2℃. The varieties of Camellia oleifera tested are Changlin No. 3 (codenamed C3), Changlin No. 4 (codenamed C4), Changlin No. 40 (codenamed C40), and Changlin No. 53 (codenamed C53). The row spacing of Camellia oleifera is 3m×4m, the density is 55 plants / mu, the age of the trees is 12 years, and they have entered the peak fruit-bearing period. Plants with good growth conditions and the same tree height and crown width of the same variety were selected. During the peak flowering period, four varieties were used for positive, reciprocal crosses and controls (natural pollination treatments), with a total of 20 pollination treatments, as shown in Table 1.

[0056] Table 1 Pollination combination design

[0057]

[0058] In November, during the peak flowering period of Camellia oleifera, flower branches with buds about to open of four varieties were collected at the Camellia oleifera demonstration base. The length of the flower branches was 20 to 30 cm. They were isolated and hydroponically cultivated in the laboratory, and pollen was collected and placed in brown glass bottles, which were placed in the refrigerator for refrigeration and taken out as needed. The buds that were about to open were emasculated, and pollinated and sealed with adhesive tape according to the complete double-row hybrid test design. The number of pollinated flowers and the total number of flowers per plant of each variety were counted. In October of the following year, the offspring fruits of each pollination combination were collected separately. Fifty fruits were randomly selected from each pollination combination for the determination of offspring traits, and all fruits with insufficient number were measured. The weight of a single fruit was measured with an electronic balance (accuracy 0.01g), and the dry seed weight and dry kernel weight were measured after drying. The oil content of the seed kernel was determined by Soxhlet extraction method, referring to GB5009.6–2016, and the results are shown in Table 2.

[0059] Dry kernel yield = dry kernel mass / dry seed mass × 100% (1)

[0060] Kernel oil content = oil mass / dry kernel mass × 100% (2)

[0061] Fresh fruit oil content = dry seed mass × dry seed kernel rate × kernel oil content / fresh fruit mass × 100% (3) Table 2 Oil content of kernels and fresh fruits of different pollination combinations

[0062]

[0063] Pollination of different varieties showed significant differences in the oil content of kernels and fresh fruits. There is a pollen direct effect on the oil content of kernels and fresh fruits in Camellia oleifera. Considering the oil content of kernels and fresh fruits, the best pollination variety of Changlin 3 and Changlin 4 is Changlin 53, the best pollination variety of Changlin 40 is Changlin 4, and the best pollination variety of Changlin 53 is Changlin 40. The flowers that are naturally pollinated during the peak flowering period are marked, and the fruit setting of each pollination combination and each variety of individual plants is counted when the fruit is ripe, and the fruit setting rate is calculated. The calculation formula for the fruit setting rate (A) of Camellia oleifera during the entire flowering period by artificial pollination is as follows. The fruit setting rates of different pollination combinations during the peak flowering period and the entire flowering period are shown in Table 3:

[0064] X = Number of fruits marked at the peak flowering stage / Number of flower buds marked at the peak flowering stage × 100% (1)

[0065] Y = number of natural fruits / number of flower buds × 100% (2)

[0066] Z = Number of pollinated fruits / number of pollinated flowers × 100% (3)

[0067]

[0068] Among them, X is the natural pollination fruit setting rate during the peak flowering period of Camellia oleifera, Y is the natural pollination fruit setting rate during the entire flowering period, and Z is the artificial pollination fruit setting rate during the peak flowering period. The flowering period of Camellia oleifera lasts for more than one month, and the flowering periods of different varieties vary. According to the requirements of the mixed test design, in order to obtain the accurate fruit setting rate of artificial pollination during the entire flowering period, it is necessary to convert it through the above equation.

[0069] Table 3 Fruit setting rate during the flowering period and the whole flowering period of different artificial pollination combinations

[0070]

[0071] The fruit setting rate in the full flowering period and the fruit setting rate in the whole flowering period were basically the same under different combinations. There was a pollen xenia effect in the fruit setting rate of oil-tea camellia. The best pollination variety of Changlin 53 was Changlin 3, and the best pollination variety of Changlin 3, Changlin 4 and Changlin 40 was Changlin 53. The oil yield per plant was calculated based on the data of flowering number, fruit setting rate, fruit quality and fresh fruit oil content as shown in Table 4. The calculation formula for the oil yield per oil-tea camellia is as follows:

[0072] Oil yield per oil-tea tree = number of flower buds × fruit setting rate (A) × single fruit weight × fresh fruit oil content

[0073] Table 4 Actual oil yield of a single tea tree during artificial pollination during the entire flowering period (kg)

[0074]

[0075]

[0076] The oil yield of a single tea plant under different mixing ratios was calculated through the mixture experiment design calculation method. Table 1 shows the yield of artificial pollination between two varieties. It is necessary to calculate the actual oil yield of different mixing ratios through forward and reverse cross transformation. For example, the oil yield of C3×C3 = (0.0952+0.095) / 2, the oil yield of C3×C4 = (0.2723+0.6363) / 2, and the oil yield of C3×C4×C40 = (0.2723+0.6363+0.2936+0.5093+0.4822+0.6021) / 6. The results are shown in Table 5.

[0077] Table 5 Actual oil yield of single tea tree during the whole flowering period (kg)

[0078]

[0079] The data of oil yield per plant in Table 5 were input into JMP software to analyze and process the test results. Figure 1This is a "predicted value - actual value" graph obtained based on JMP software. The red solid line in the figure is the predicted value given by the software, and each point is the actual measured test value. RSq = 0.93, P = 0.0205 < 0.05, and the fitting is accurate.

[0080] Table 6 shows the parameter estimation values. It can be seen from the table that C3×C40 (reciprocal cross), C4×C40 (reciprocal cross) and C3×C53 (reciprocal cross) have significant effects on the oil yield per plant, and C4×C53 (reciprocal cross) and C40×C53 (reciprocal cross) have extremely significant effects on the oil yield per plant. The following formula is the fitting equation for the effect of different variety configurations on the oil yield per plant. This equation can also be used to predict the oil yield per plant of each variety at any value within its value range.

[0081] F(X)=0.1130X1+0.1484X2+0.1121X3+0.2009X4+0.9833X1X2+1.1058X1X3+1.3648X2X3+1.4

[0082] 941X1X4+1.7591X3X4+1.8208X3X4.

[0083] Where F(X) is the oil yield per plant, X1 is C3 (mixed), X2 is C4 (mixed), X3 is C40 (mixed), and X4 is C53 (mixed).

[0084] Table 6 Parameter estimates

[0085]

[0086] As attached Figure 2 As shown in the figure, the prediction profiler can predict the oil yield per plant of the four varieties at any value within their value range. Within the given range, as C3 increases, the oil yield per plant gradually decreases; as C4, C40 and C53 increase, the oil yield per plant will first increase and then decrease, and the peak value appears within its value range. Within the given reaction condition range, the configuration ratio of the four varieties is Changlin No. 3 (6.16%) + Changlin No. 4 (27.46%) + Changlin No. 40 (27.12%) + Changlin No. 53 (39.26%), at which time the oil yield per plant is the highest (0.7134 kg), which is converted to 39.24 kg per mu of oil.

[0087] The above is only an embodiment of the present invention. The common sense such as the known specific structure and characteristics in the scheme is not described in detail here. The ordinary technicians in the relevant field know all the common technical knowledge in the technical field of the invention before the application date or priority date, can obtain all the existing technologies in the field, and have the ability to apply the conventional experimental means before that date. The ordinary technicians in the relevant field can improve and implement this scheme in combination with their own abilities under the enlightenment given by this application. Some typical known structures or known methods should not become obstacles for ordinary technicians in the relevant field to implement this application. It should be pointed out that for those skilled in the art, without departing from the structure of the present invention, several deformations and improvements can be made, which should also be regarded as the protection scope of the present invention, which will not affect the effect of the implementation of the present invention and the practicality of the patent. The protection scope required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. A method for planting oil-tea-pollinating trees, comprising the following steps: S1. Select Changlin Camellia oleifera series as the test materials; S2. Four oil-tea camellia varieties were subjected to artificial self-pollination, cross-pollination and natural pollination to design 20 pollination ratio combinations, and statistically analyzed the relevant data of oil-tea camellia fruits and seeds in different combinations, including the quantity, quality, oil content and fruit setting rate of fruits and seeds; S3. Statistical calculation of natural pollination fruiting rate X, natural pollination fruiting rate Y, artificial pollination fruiting rate Z, calculated the artificial pollination fruiting rate A = (Z × Y) / X, to obtain the actual oil yield of a single tea plant artificial pollination during the entire flowering period; S4. The oil yield of a single camellia plant under different mixture ratios was calculated by the mixture test design calculation method, and the oil yield data of a single camellia plant was input into the JMP software. The optimal variety configuration was predicted by the JMP software, and the JMP software was fitted with a predicted value-actual value image; S5. Using Origin software, a linear fitting equation was made to show the effect of different mixing ratios on the oil-tea camellia yield. The linear fitting equation can be used to calculate the oil yield of each oil-tea camellia plant within its value range for each variety. S6. predicting the influence of the tea varieties on the oil yield of a single tea plant in the mixture ratio test within the value range through the prediction profiler; S7. Calculate the optimal pollination mixture ratio combination to obtain the best planting plan for oil tea pollination trees.

2. The method for planting oil-tea-pollinating trees according to claim 1, characterized in that: In S1, the varieties of tea oil test materials are Changlin No. 3, Changlin No. 4, Changlin No. 40 and Changlin No.

53. Among them, the tea oil test materials are selected from plants with good growth conditions and consistent tree height and crown width of the same variety.

3. The method for planting oil-tea-pollinating trees according to claim 2, characterized in that: In S2, mature camellia oleifera fruits were collected from the middle and upper part of the outer crown and multiple indicators of the camellia oleifera fruits were measured, including the quality, quantity, kernel oil content and fresh fruit oil content of the fruits and seeds; within 24 hours of fruit harvest, the fresh fruit quality and dry seed quality were measured using an electronic balance; the fresh camellia oleifera seeds were placed in a 60°C oven and dried to constant weight, and the dry seed quality and dry kernel quality were weighed; the kernel oil content was measured using the Soxhlet extraction method, and the calculation method was as follows: Dry kernel yield = dry kernel mass / dry seed mass × 100%; Seed kernel oil content = oil mass / dry kernel mass × 100%; Fresh fruit oil content = dry seed mass × dry seed kernel rate × kernel oil content / fresh fruit mass × 100%.

4. The method for planting oil-tea-pollinating trees according to claim 3, characterized in that: In S3, during the flowering period, the buds that are about to open are emasculated during artificial pollination. According to the complete diallel hybridization test design, pollination and tape sealing are performed, and the number of pollinated flowers and the total number of flowers per plant of each variety are counted. At the same time, the flowers that are naturally pollinated during the flowering period are marked. When the fruits are ripe, the fruit setting of each pollination combination and each variety per plant is counted to calculate the fruit setting rate. The calculation formula for the artificial pollination fruit setting rate A of oil tea during the entire flowering period is as follows: X = number of marked fruits at the peak flowering stage / number of marked buds at the peak flowering stage × 100%; Y = number of natural fruits / number of flower buds × 100%; Z = number of pollinated fruits / number of pollinated flowers × 100%; Among them, X is the natural pollination fruit setting rate during the peak flowering period of Camellia oleifera, Y is the natural pollination fruit setting rate during the entire flowering period, and Z is the artificial pollination fruit setting rate during the peak flowering period.

5. The method for planting oil-tea-pollinating trees according to claim 4, characterized in that: In S3, the calculation formula for the oil yield of a single tea tree is as follows: Oil yield of a single tea tree = number of flower buds × fruit setting rate × single fruit weight × fresh fruit oil content.

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