Application of a composition in preparing a supplementary mixture for improving endurance, explosive power and muscle mass at the same time and product thereof

Abstract

The application of a composition of the present invention in preparing a supplementary mixture for improving endurance, explosive power and muscle mass at the same time and the product can significantly improve physical fitness level at the same time, enhance skeletal muscle mass and strength in high-intensity training, and improve sports endurance under high-intensity training through the synergistic effect of succinic acid, glutamic acid and arginine.

Description

Technical Field

[0001] The invention relates to an application of a composition in preparing a supplementary mixture for improving endurance, explosive power and muscle mass at the same time and a product. Background Art

[0002] With the changes in the world's new military, the future war form and combat mechanism will undergo unprecedented changes. Future wars will be more global in combat space and more multidimensional in combat paradigm. The rapidity, suddenness and intensity of wars are increasing day by day, which puts forward new requirements for the rapid generation, maintenance and enhancement of the core combat fitness of individual soldiers. Physical fitness is an important cornerstone of the combat effectiveness of the army. The goal of modern military physical training is high intensity and comprehensiveness. However, in the context of a complex military environment, continuous high-intensity and long-term training / combat will easily cause fatigue to soldiers, causing homeostasis disorders, oxidative stress and metabolic changes in the body, resulting in a decrease in the combat effectiveness of individual soldiers.

[0003] Therefore, developing new and efficient physical fitness enhancers to quickly improve soldiers' skeletal muscle function and reduce stress response during training / combat is an urgent need to improve individual soldiers' physical fitness, and is also a key issue in improving the combat effectiveness of troops and ensuring the ability to win.

[0004] In this technical field, the evaluation indicators for products that improve stress response to high-intensity training and skeletal muscle metabolism include endurance, explosive power and muscle mass. A product must be able to meet the three evaluation indicators of endurance, explosive power and muscle mass at the same time to be considered to have a good regulatory effect on stress response to high-intensity training and improvement of skeletal muscle metabolism. Summary of the invention

[0005] The technical problem to be solved by the present invention is how to effectively improve the stress response and skeletal muscle metabolism of soldiers during high-intensity training, thereby reducing fatigue and improving combat effectiveness.

[0006] In order to solve the above technical problems, the present invention discloses an application of a composition in the preparation of a supplementary mixture for improving endurance, explosive power and muscle mass at the same time, wherein one portion of the composition consists of 0.2 g succinic acid, 4 g glutamic acid, 6 g arginine and auxiliary materials.

[0007] Preferably, the succinic acid is derived from succinic acid monoglyceride.

[0008] Preferably, the glutamate is derived from sodium glutamate.

[0009] Preferably, the arginine is L-arginine.

[0010] Preferably, the auxiliary materials are excipients and flavoring agents.

[0011] More preferably, the supplement mixture is in the form of unit packaging, each unit packaging containing one or more portions of the energy-enhancing compound.

[0012] Other nutrients may also be added to the supplement mixture, but the added nutrients do not contain succinic acid, glutamic acid and arginine.

[0013] In practice, we can apply a supplemental mixture that can be prepared to improve endurance, explosive power and muscle mass at the same time.

[0014] In the present invention, succinic acid, glutamic acid and arginine act synergistically, and after combined use, they can significantly improve physical fitness levels, enhance skeletal muscle mass and strength in high-intensity training, and improve sports endurance, explosive power and skeletal muscle mass under high-intensity training. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 Schematic diagram of the process of high-intensity interval training (HIIT) and resistance training experiments in mice.

[0016] Figure 2 Schematic diagram of the changes in skeletal muscle mass in mice during high-intensity interval training.

[0017] Figure 3 Schematic diagram of the changes in grip strength of mice during high-intensity intermittent training.

[0018] Figure 4 Schematic diagram of the endurance changes of mice during high-intensity interval training.

[0019] Figure 5 Schematic diagram of the mouse impedance training animal model.

[0020] Figure 6 Schematic diagram of the changes in skeletal muscle mass in mice during resistance training.

[0021] Figure 7 Schematic diagram of the changes in the grip strength of mice during resistance training.

[0022] Figure 8 Schematic diagram of the changes in endurance of mice during resistance training. DETAILED DESCRIPTION

[0023] In order to better understand the present invention, the present invention is further described below in conjunction with specific embodiments.

[0024] Example 1 Experiment on the effect of high-intensity intermittent training on improving the physical phenotype of mice

[0025] The experimental mice were randomly divided into a control group, an arginine group, a glutamate group, a succinic acid group and a physical fitness enhancement supplement group, with 10 mice in each group.

[0026] The experimental mice were subjected to high-intensity intermittent training (HIIT) according to the high-intensity intermittent training program shown in Table 1. Figure 1 The experiment was conducted according to the experimental flow chart shown in the figure, wherein: ① 5 days of adaptive training; ② weighing and evaluating physical phenotype; ③ as shown in Table 2, each experimental group of mice was gavaged with normal saline, arginine, glutamate, succinic acid and physical fitness supplement mixture every morning; ④ high-intensity interval training (HIIT) for mice, which can be specifically described as follows: all mice underwent 5 days of adaptive training (speed 10 m / min, 15-20 min / d, slope of 0°), and the baseline weight and physical fitness phenotype (grip strength, endurance) of mice were assessed before formal HIIT, and then the control group, arginine group, glutamate group, succinic acid group and physical fitness supplement mixture group shown in Table 2 were gavaged every morning for 21 days, and HIIT was performed at the same time, 5 times / week, and the weight and physical fitness level of mice were detected 7, 14, and 21 days after training.

[0027] Table 1: High-intensity interval training (HIIT) training program

[0028]

[0029] Table 2:

[0030]

[0031] Observation indicators: ⑴ Body composition of mice: The body weight and lean body mass of mice were detected by live mouse body composition analyzer (Newmai, QMR06-090H); the wet weight of gastrocnemius muscle and gastrocnemius mass index of mice were evaluated by weighing method. ⑵ Physical phenotype: The mouse grip strength test system (Calvin, KW-ZL) was used to detect the grip strength of mice (the average of the maximum grip strength of 3 times was taken); the small animal treadmill (Calvin, KW-PT) was used to evaluate the endurance of mice-exhaustive running time (starting at 10m / min, then increasing the speed by 1m / min every 1min until physical exhaustion, that is, the mouse failed to continue running for 3 consecutive times, and the time was recorded).

[0032] The test results are shown in Figures 2 to 4 , where *: Dunnett's multiple test, p<0.05; **: Dunnett's multiple test, p<0.005.

[0033] According to the test results, it can be seen that the physical fitness enhancing composition can significantly increase the skeletal muscle mass of high-intensity trained mice and improve grip and exercise endurance at the same time. When succinic acid, arginine, and glutamic acid are used alone, they cannot achieve the simultaneous increase of skeletal muscle mass and improvement of grip and exercise endurance, and there is no significant difference between them and the control group. However, at this low dose, the three work synergistically, thereby significantly increasing the skeletal muscle mass of high-intensity trained mice and improving grip and exercise endurance at the same time. Example 2

[0034] according to Figure 5 The training protocol shown in the figure was used to perform resistance training on experimental mice. The training was performed using a small animal voluntary activity wheel system (Calvin, KW-ZL). At the beginning of the experiment, the resistance of the wheel was increased to 1g and then increased by 1g per week until it reached 3g. Figure 1 The experiment was conducted according to the experimental flow chart shown in the figure, wherein: ① 5 days of adaptive training; ② weighing and evaluating physical phenotype; ③ as shown in Table 2, each experimental group of mice was gavaged with normal saline, arginine, glutamate, succinic acid and physical fitness supplement mixture every morning; ④ mouse impedance training (RT); specifically, it can be described as: all mice were subjected to 5 days of adaptive wheel training, and the baseline weight and physical fitness phenotype (gripping strength, endurance) of mice were evaluated before the formal RT, and then the control group, arginine group, glutamate group, succinic acid group and physical fitness supplement mixture group were gavaged with the preparations shown in Table 2 every morning, for a total of 21 days, and RT was performed at the same time, 2 km / day, 5 days / week, and the weight and physical fitness level of mice were detected 7, 14, and 21 days after training, respectively.

[0035] Observation indicators: ⑴ Body composition of mice: The body weight and lean body mass of mice were detected by live mouse body composition analyzer (Newmai, QMR06-090H); the wet weight of gastrocnemius muscle and gastrocnemius mass index of mice were evaluated by weighing method. ⑵ Physical phenotype: The mouse grip strength test system (Calvin, KW-ZL) was used to detect the grip strength of mice (the average of the maximum grip strength of 3 times was taken); the small animal treadmill (Calvin, KW-PT) was used to evaluate the endurance of mice-exhaustive running time (starting at 10m / min, then increasing the speed by 1m / min every 1min until physical exhaustion, that is, the mouse failed to continue running for 3 consecutive times, and the time was recorded).

[0036] The test results are shown in Figures 6 to 8 , where *: Dunnett's multiple test, p<0.05; **: Dunnett's multiple test, p<0.005.

[0037] According to the test results, it can be seen that the physical fitness enhancing composition can significantly increase the skeletal muscle mass of high-intensity trained mice and improve grip and exercise endurance at the same time. When succinic acid, arginine, and glutamic acid are used alone, they cannot achieve the simultaneous increase of skeletal muscle mass and improvement of grip and exercise endurance, and there is no significant difference between them and the control group. However, at this low dose, the three work synergistically, thereby significantly increasing the skeletal muscle mass of high-intensity trained mice and improving grip and exercise endurance at the same time.

[0038] It can be seen from the above experiments that, whether it is high-intensity intermittent training (HIIT) or resistance training, the physical fitness enhancement supplement disclosed in the present invention shows significant or extremely significant differences compared with the control group when arginine, glutamic acid, and succinic acid are administered alone, and can simultaneously improve endurance, explosive power, and muscle mass, meeting the requirements of physical fitness enhancement and recovery nutritional supplements during high-intensity training.

[0039] The above is a specific implementation of the present invention. It should be noted that, for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications are also considered to be within the protection scope of the present invention.

Claims

1. Use of a composition in the preparation of a supplement for improving endurance, explosive power and muscle mass at the same time, characterized in that: One serving of the composition consists of 0.2 g succinic acid, 4 g glutamic acid, 6 g arginine and excipients.

2. Use of a composition according to claim 1 in the preparation of a supplement for improving endurance, explosive power and muscle mass at the same time, characterized in that: The arginine refers to L-arginine.

3. Use of a composition according to claim 1 or 2 in the preparation of a supplementary mixture for improving endurance, explosive power and muscle mass at the same time, characterized in that: The auxiliary materials include excipients and flavoring agents.

4. Use of a composition according to claim 1 or 2 in the preparation of a supplementary composition for improving endurance, explosive power and muscle mass at the same time, characterized in that: The supplement mixture is in the form of unit packaging, and each unit packaging contains one or more compositions.

5. Use of a composition according to claim 3 in the preparation of a supplement for improving endurance, explosive power and muscle mass at the same time, characterized in that: The supplement mixture is in the form of unit packaging, and each unit packaging contains one or more compositions.

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