A device and method for assisting culture and examination of a liver cancer animal model

Abstract

The application provides a device and method for auxiliary culture and detection of a liver cancer animal model, which comprises a box, an experimental mouse culture and detection room in the box, a fixing cavity in the culture and detection room, a fixing assembly in the fixing cavity, a pair of parallel and opposite side plates, a reversible clamping plate vertically connected between the pair of side plates and used for fixing an experimental mouse, an inclined plate rack horizontally and obliquely arranged and used for supporting and fixing the side plates, a support plate parallel arranged longitudinally below the clamping plate, a head pinching rod and a trunk pinching rod installed on the support plate and used for pinching and fixing the skin of the experimental mouse, a weighing cavity connected with the fixing assembly through a moving assembly, and a detection cavity comprising diagnostic equipment, wherein the experimental mouse is transferred to the longitudinal direction below the diagnostic equipment through the moving assembly for detection and diagnosis.

Description

Technical Field

[0001] This invention relates to the field of animal model research technology for human diseases, specifically to a device and method for auxiliary culture and testing of liver cancer animal models. Background Technology

[0002] Liver cancer is one of the most common tumors in humans, characterized by its insidious onset, short course, and extremely high morbidity and mortality. Establishing a suitable animal model of liver cancer is a crucial prerequisite for diagnostic research in humans, and inducing liver cancer in mice through gavage or intraperitoneal injection is a common method for understanding and diagnosing liver cancer.

[0003] Chinese patent CN201810450157.2 discloses a fixation frame for gavage in medical experimental mice. One end of the fixation plate is placed on a base plate, which has a buffer groove. The other end of the fixation plate has a sliding groove. Two arc-shaped clamping plates are placed on opposite sides of the fixation plate, with one end connected to the base plate. The inner side of the arc-shaped clamping plates has a fixing groove. Two edge wing plates are placed on the other side of the two arc-shaped clamping plates, with one end connected to the base plate. The outer side of the edge wing plates has two slots, arranged vertically. The main clamping block is engaged in the upper slot. Inside, the auxiliary locking block is placed in the lower locking groove. The two sides of the main fixing belt are connected to the two main locking blocks by elastic strips, and the two sides of the auxiliary fixing belt are connected to the two auxiliary locking blocks by elastic strips. The fixing plate has a moving groove and is close to the sliding groove. The connecting rod passes through the moving groove. The sliding block is placed on one end of the connecting rod and is located inside the fixing plate. The sliding block has an arc-shaped block structure and its inner side faces the main fixing belt. The adjusting block is placed on the other end of the connecting rod. One side of the support plate is placed outside the sliding block. The support plate has an arc-shaped plate structure and its other side extends into the sliding groove.

[0004] When administering medication to laboratory mice via gavage, it is necessary to observe the rise and fall of the mouse's neck and abdomen to prevent the gavage device from entering the trachea and to determine whether the device has reached the stomach. Although the above-mentioned technical solution is simple in structure and can effectively improve the efficiency of medication administration to laboratory mice, the arc-shaped restraint device that fixes the mouse's neck and abdomen can obstruct the experimenter's observation of the mouse, which is not conducive to the diagnosis of gavage administration.

[0005] Chinese Patent CN202120065319.8 discloses a convenient gavage mechanism for laboratory mice, comprising a fixing plate, a positioning device, a support device, and a placement groove. The positioning device includes a first clamp, a first cavity, a spring, a limiting rod, a limiting block, a first rectangular hole, a second cavity, a limiting plate, a bolt, a second rectangular hole, a second clamp, a third cavity, a screw, a knob, a slider, and a third rectangular hole. The support device includes a support rod, a first connecting rod, a second connecting rod, a slot, and a buckle. By setting up the positioning device, laboratory mice of different sizes or species can be restrained and fixed, and the operation is simple. By setting up the support device, the experimenter can fix the laboratory mouse on a table, or lift the laboratory mouse by reversing the support device before performing the gavage operation. This ensures that the laboratory mouse will not harm the experimenter while making the gavage operation more flexible. Although this technical solution avoids harm to the experimenters by lifting the experimental mice with a reversing support device before gavage, the clamps above the placement slot of the fixing device still partially obstruct the observation of the experimental mice, which is not conducive to the observation of the experimental mice, and at the same time, it does not provide an environment for the culture and testing of experimental mice.

[0006] Therefore, it is crucial to design and research a device that can both enable unobstructed fixation of experimental mice and provide an environment for researchers to culture, dissect, and examine the mice. Summary of the Invention

[0007] The technical problem to be solved by the present invention is how to provide a device for unobstructed fixation of experimental mice, which meets the requirements of experimental personnel for the culture and testing of experimental mice.

[0008] To address the aforementioned technical problems, this invention provides a device for auxiliary culture and testing of a liver cancer animal model, comprising a housing, wherein the housing includes a mouse culture and testing chamber, and the culture and testing chamber includes:

[0009] The fixing cavity includes a fixing assembly, which includes a pair of parallel and opposite side plates, a flip-up clamping plate vertically connected between the pair of side plates for fixing the experimental mouse, a horizontally inclined frame for supporting and fixing the side plates, a support plate parallel to the clamping plate in the longitudinal direction below, and a head pinching rod and a torso pinching rod installed on the support plate for pinching and fixing the skin of the experimental mouse.

[0010] Weighing chamber, which is connected to the fixed component via a movable component; and

[0011] The examination chamber includes diagnostic instruments, and the laboratory mouse is moved longitudinally below the diagnostic instruments via the moving component for examination and diagnosis.

[0012] Furthermore, a support bracket for supporting the arm of the experimenter is provided on one side of the top edge of the fixed cavity.

[0013] Furthermore, a drive shaft is connected between the clamping plate and the side plate, and a rotating disk is sleeved on the drive shaft.

[0014] Furthermore, the clamping plate is provided with an adjustment groove.

[0015] Furthermore, a gear transmission mechanism with rounded corners is installed on the inner sides of the pair of side plates. The gear transmission mechanism is vertically connected to the transmission rod and the support plate. A telescopic component is vertically arranged in the middle of the support plate for longitudinal displacement of the support plate to contact the back of the experimental mouse.

[0016] Furthermore, a parallel moving groove and a positioning groove are provided at the longitudinal middle position of the pair of side plates. The transmission rod passes through the moving groove and is connected to a transmission handle. A positioning rod is rotatably connected to the transmission handle and inserted into and fixed in the positioning groove.

[0017] Furthermore, the gear transmission mechanism includes a driving member and a driven member that mesh with each other, as well as a linkage member connected to the driving member via a transmission belt.

[0018] Furthermore, the moving component includes a locking cover and a lifting platform that is vertically fixed to one side of the locking cover. The lifting platform is vertically mounted on a horizontal moving guide rail and moves horizontally.

[0019] Furthermore, the locking cover and the slot are inserted and fixed together, with the slot located on one side of the fixing component.

[0020] Preferably, the inspection chamber further includes a disinfection component, which is evenly distributed at intervals along the top edge inside the locking cover, for disinfecting and sterilizing the fixing component.

[0021] Preferably, the chamber further includes a first comparison chamber and a second comparison chamber.

[0022] Preferably, the outer casing includes:

[0023] A clean room, located on one side of the outside of the chamber, is used to disinfect external equipment entering the culture and testing room;

[0024] The waste liquid storage chamber includes a liquid collection chamber disposed on one side of the outer side of the box body. The liquid collection chamber is connected to the cleaning chamber and the fixed chamber via a conduit.

[0025] Furthermore, the cleaning chamber has a pull-out structure.

[0026] Furthermore, an operating port is provided on one side of the box, and a hand protective device is provided at the operating port.

[0027] Preferably, the side wall of the enclosure is provided with an air vent.

[0028] Furthermore, an observation window is provided on the top of the enclosure; and an enclosure door is provided on the side wall of the enclosure.

[0029] A method for auxiliary culture and testing of a liver cancer animal model, comprising an apparatus for auxiliary culture and testing of a liver cancer animal model, the method comprising the following steps:

[0030] S1. Observation and culture: The experimental mice are placed inside the culture and testing chamber for observation and culture before constructing the liver cancer model;

[0031] S2. Back-facing fixation: When it is necessary to construct a liver cancer model by gavage and intraperitoneal injection on experimental mice, the experimenter fixes the experimental mouse to the clamping plate by pinching the back of the experimental mouse;

[0032] S3. Flip and observe: Start the drive source to drive the clamping plate to flip half a turn so that the experimental mouse's face is facing upwards;

[0033] S4. Layered fixation: The support plate is lifted by the telescopic component, so that the head pinch rod and the torso pinch rod on the inner side of the support plate come into contact with the back of the experimental mouse and rotate in opposite directions, thereby restricting and fixing the skin and muscles of the experimental mouse's back by the rotating head pinch rod and torso pinch rod.

[0034] S5. Assisted gavage: After the experimental mouse is fixed, the fixed component is used to tilt the head of the fixed experimental mouse upward. With the support support that supports the experimenter's arm, the gavage operation is completed on the experimental mouse with its head tilted upward.

[0035] S6. Inspection and diagnosis: The fixing component is locked by the locking cover, so that the diagnostic instrument above the locking cover can inspect and diagnose the experimental mouse in the fixing component.

[0036] Compared with the prior art, the present invention has the following beneficial effects:

[0037] 1. This invention uses a control panel on the side wall of the enclosure to raise the telescopic components, bringing the support plate, head pinch rod, and torso pinch rod into contact with the back of the experimental mouse. Then, by rotating the transmission handle counterclockwise with the right hand, the linkage drives the two sets of torso pinch rods and head pinch rods on the inner side of the support plate to rotate in opposite directions. This causes the skin on the mouse's back to be rolled, pinched, and fixed. The design of the head pinch rod having a larger diameter than the torso pinch rod allows them to fit more closely to the mouse's head, neck, and back, effectively rolling and pinching the mouse's head and back. This reduces discomfort and struggling during gavage, minimizing pressure damage to the mouse's internal organs caused by the gavage device. The larger diameter of the head pinch rod also relatively elevates the mouse's head, reducing the difficulty of gavage. Furthermore, placing the right hand on the support and tilting the fixing components further facilitates the gavage procedure.

[0038] 2. This invention uses a fixing component to fix and rotate laboratory mice for dissection. After dissection, a moving component moves a lifting component closer to the weighing chamber, aligning the locking cover on the side of the lifting component with the top of the slot. The lifting component then descends, locking the locking cover into the slot, aligning the transparent plate inside the locking cover with the top of the fixing component. This allows a diagnostic tool located at the top of the locking cover to examine and diagnose the liver of the laboratory mouse through the transparent plate. This allows the culture and dissection examination and diagnosis of the laboratory mouse to be performed in the same environment, thus reducing the interference of the laboratory mouse's environment on the diagnostic results. Furthermore, the disinfection component inside the locking cover disinfects the fixing component after the laboratory mouse is removed, facilitating subsequent reuse. Attached Figure Description

[0039] Figure 1 This is a front view of the device of the present invention;

[0040] Figure 2 This is a side view of the device of the present invention;

[0041] Figure 3 This is a top view of the device of the present invention;

[0042] Figure 4 This is a schematic diagram of the structure of the fixed cavity of the device of the present invention;

[0043] Figure 5 This is a schematic diagram of the fixing assembly of the fixing cavity in the device of the present invention;

[0044] Figure 6 This is an enlarged view of the structure of the fixing assembly of the fixing cavity in the device of the present invention;

[0045] Figure 7This is an enlarged view of the clamping plate of the fixing assembly of the fixing cavity in the device of the present invention;

[0046] Figure 8 This is a schematic diagram of the head pinching rod and the torso pinching rod of the fixing component of the fixing cavity of the device of the present invention.

[0047] Figure 9 This is a schematic diagram of the gear transmission mechanism of the fixing component of the fixing cavity in the device of the present invention;

[0048] Figure 10 This is a schematic diagram of the structure of the inspection chamber of the device of the present invention;

[0049] Figure 11 This is a schematic diagram of the structure of the cleaning chamber of the device of the present invention;

[0050] Figure 12 This is an enlarged view of the structure of the cleaning chamber of the device of the present invention;

[0051] Figure 13 This is a flowchart illustrating the operation method of the device of the present invention.

[0052] In the diagram: 1. Box body; 1a. Observation table; 1b. Box door; 1c. Control panel; 1d. Ventilation port; 1e. Operation port; 1e1. Hand protective gear; 11. Culture and testing chamber; 11a. Fixed cavity; 11a1. Side plate; 11a11. Moving slot; 11a12. Positioning slot; 11a2. Clamping plate; 11a21. Adjustment slot; 11a22. Connecting plate; 11a23. Insertion slot; 11a24. Clamping slot; 11a25. External connection slot; 11a26. Closing block; 11a3. Support plate; 11a31. Head skin pinching rod; 11a32. Torso skin pinching rod; 11a33. Telescopic component; 11a4. Inclined frame; 11a5. Support Support; 11a6, Drive shaft; 11a61, Rotating disk; 11a7, Transmission rod; 11a71, Transmission handle; 11a72, Positioning rod; 11a8, Gear transmission mechanism; 11a81, Driving component; 11a82, Driven component; 11a83, Linkage component; 11b1, Locking cover; 11b2, Lifting platform; 11c, Inspection chamber; 11c1, Diagnostic instruments; 11c2, Disinfection component; 12, First comparison chamber; 13, Second comparison chamber; 2, Cleaning chamber; 21, Disinfection chamber; 211, Sealing plate; 22, Disinfection component; 23, Rolling rod; 24, Constraint seat; 241, Semi-open circular sleeve; 25, Traction rail; 3, Waste liquid storage chamber; 31, Liquid collection chamber. Detailed Implementation

[0053] To make the technical solutions and effects of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are some embodiments of the present invention, but not all embodiments.

[0054] like Figure 1 The diagram shows a front view of the device of the present invention. The present invention provides a device for auxiliary culture and testing of a liver cancer animal model, comprising a housing 1, with a culture and testing chamber 11 inside the housing 1; a clean chamber 2 and a waste liquid storage chamber 3 are connected to the outside of the housing 1 and communicate with it. The waste liquid storage chamber 3 is vertically connected to the clean chamber 2 below its longitudinal direction via a conduit. An operation panel 1c is provided on one side wall of the housing 1, and two operation ports 1e are provided below the operation panel 1c, with hand protective devices 1e1 installed at the operation ports 1e. An observation platform 1a is provided on the top of the housing 1, and a housing door 1b is opened on the front side of the housing 1.

[0055] Cleanroom 2 is used to disinfect items (such as enemas or syringes) that enter chamber 1, thereby reducing the impact of external equipment on the internal sterile environment after entering the culture and testing room 11.

[0056] Waste liquid storage chamber 3 is used to collect waste liquid from clean chamber 2 and culture and testing chamber 11 inside box 1.

[0057] like Figure 2 The side view of the device of the present invention shown shows that an air vent 1d is provided on the upper side wall of the housing 1. The cleaning chamber 2 is arranged perpendicular to the housing 1 and has a pull-out structure.

[0058] like Figure 3 The top view of the device of the present invention shown shows that a culture and testing chamber 11 is provided inside the box 1, and a first comparison chamber 12 and a second comparison chamber 13 are provided adjacent to the culture and testing chamber 11.

[0059] The culture and testing chamber 11 includes a fixed chamber 11a, a weighing chamber adjacent to the fixed chamber 11a, and a testing chamber 11c. A support bracket 11a5 for supporting the arm of the experimenter is provided on one side of the top edge of the fixed chamber 11a.

[0060] like Figure 4 , 5The schematic diagram of the fixing cavity 11a of the device of the present invention shown includes a fixing assembly, which includes: a pair of parallel and opposite side plates 11a1, a flip-able clamping plate 11a2 vertically connected between the pair of side plates 11a1 for fixing the experimental mouse, a horizontally inclined plate frame 11a4 for supporting the fixing side plates 11a1, a support plate 11a3 parallel to the longitudinal lower part of the clamping plate 11a2, and a head pinching rod 11a31 and a torso pinching rod 11a32 for pinching and fixing the skin of the experimental mouse installed on the support plate 11a3.

[0061] like Figure 6 , 7 The diagram shows the structure of the clamping plate of the fixing assembly of the fixing cavity 11a of the device of the present invention. A drive shaft 11a6 connects the clamping plate 11a2 to the side plates 11a1. A rotating disk 11a61 is sleeved on the drive shaft 11a6. A drive source is installed on the upper side wall of one side plate, and the drive source drives the clamping plate 11a2 to rotate. Preferably, the drive source is a DC geared motor controlled by a PLC encoder, and the DC geared motor is externally powered (not shown in the attached diagram). During operation, the drive source is activated to rotate the clamping plate 11a2 half a turn, so that the experimental mouse's face is upward. The space between the clamping plates 11a2 does not obstruct the abdomen and neck of the experimental mouse, allowing researchers to observe the real-time reactions of the experimental mouse during gavage and intraperitoneal injection without restricting the abdomen and neck, reducing the strain on the experimental mouse's gastrointestinal tract caused by constraint and compression.

[0062] The clamping plate 11a2 in the fixing assembly has symmetrical adjustment grooves 11a21 on its left and right sides. The adjustment grooves 11a21 adjust the spacing of the clamping plates 11a2 according to the size of the experimental mice. A placement groove 11a23 is opened in the middle of the vertical connecting plate 11a22 of the adjustment groove 11a21. A clamping groove 11a24 is arranged perpendicularly to the connecting plate 11a22. A closing block 11a26 is provided in the outer groove 11a25 parallel to the placement groove 11a23. The closing block 11a26 is preferably made of elastic rubber material. During operation, the experimenter holds the experimental mouse by its back to fix the mouse in the clamping plate 11a2.

[0063] like Figure 9The diagram shows the structure of the gear transmission mechanism 11a8 of the fixing assembly of the fixing cavity of the device of the present invention. A pair of side plates 11a1 in the fixing assembly are fitted with opposing gear transmission mechanisms 11a8 in the shape of rounded triangles. Each gear transmission mechanism 11a8 includes a meshing driving member 11a81 and a driven member 11a82, and a linkage member 11a83 connected to the driving member 11a81 via a transmission belt. A transmission rod 11a7 and a support plate 11a3 are vertically connected between the two gear transmission mechanisms 11a8. A telescopic member 11a33 is vertically arranged at the middle of the support plate 11a3. The telescopic member 11a33 is used to adjust the longitudinal displacement of the support plate 11a3 so that the support plate 11a3 contacts the back of the experimental mouse. Preferably, the telescopic member 11a33 is a PLC-controlled electric telescopic rod.

[0064] A moving groove 11a11 and a positioning groove 11a12 are provided at the longitudinal middle position of a pair of side plates 11a1. A transmission rod 11a7 passes through the moving groove 11a11 and is connected to a transmission handle 11a71. A positioning rod 11a72 is rotatably connected to the transmission handle 11a71 and is inserted and fixed in the positioning groove 11a12.

[0065] like Figure 8 The schematic diagram shows the structure of the head pinching rod 11a31 and the torso pinching rod 11a32 of the fixing assembly of the fixing cavity 11a of the device of the present invention. The design that the diameter of the head pinching rod 11a31 is larger than that of the torso pinching rod 11a32 allows the head pinching rod 11a31 and the torso pinching rod 11a32 to fit more closely to the back of the head, neck and torso of the experimental mouse, so that the head pinching rod 11a31 and the torso pinching rod 11a32 can roll and pinch to restrict the head and back of the experimental mouse. This reduces the discomfort and struggle caused by the experimental mouse during gavage, which can lead to compression damage to the internal organs of the experimental mouse. At the same time, the design that the diameter of the head pinching rod 11a31 is larger than that of the torso pinching rod 11a32 also makes the head of the experimental mouse relatively higher, thereby reducing the difficulty of gavage.

[0066] like Figure 10 The schematic diagram of the inspection chamber 11c of the device of the present invention is shown. A movable component is installed on the bottom surface of the inspection chamber 11c. The top of the movable component is movably connected to the side platform via a lifting platform 11b2. The lifting platform 11b2 is vertically mounted on a horizontal moving guide rail and moves horizontally. A locking cover 11b1 is fixedly connected to the side of the side platform. Disinfection components 11c2 are evenly distributed at intervals on the top edge of the locking cover 11b1 for disinfecting and sterilizing the fixed component. A transparent plate is provided in the middle of the locking cover 11b1. The locking cover 11b1 is movably connected to the transfer block via an adjusting seat. A column is installed on the top of the transfer block, and diagnostic instruments 11c1 are installed on the column.

[0067] The culture and anatomical examination of the laboratory mice are carried out in the same environment, thereby reducing the interference of the laboratory mice's environment on the examination and diagnosis results. The disinfection component 11c2 set on the locking cover 11b1 will disinfect the fixing components of the laboratory mice after the diagnosis is completed, so as to facilitate subsequent reuse.

[0068] like Figure 11 , 12 The schematic diagram of the structure of the cleaning chamber 2 of the device of the present invention is shown. The cleaning chamber 2 is connected to the culture and testing chamber 11 inside the box 1. The cleaning chamber 2 includes a disinfection chamber 21 and a sealing plate 211. Traction rails 25 are symmetrically installed on the front and rear side walls of the disinfection chamber 21. A constraint seat 24 is fixedly connected to the inner side of the disinfection chamber 21. A semi-open circular sleeve 241 is installed on the top of the constraint seat 24. The semi-open circular sleeve 241 is preferably made of elastic plastic, which facilitates squeezing and fixing the gavage device or the marker pen inside the semi-open circular sleeve 241.

[0069] like Figure 13 The flowchart shown is a schematic diagram of the operation method of the device of the present invention, which includes the following steps:

[0070] Step 1, observation and culture: The experimental mice are placed inside the culture and testing chamber 11 for observation and culture before constructing the liver cancer model;

[0071] Step 2, Back-facing fixation: When it is necessary to construct a liver cancer model by gavage and intraperitoneal injection on experimental mice, the experimenter wears a protective handgear 1e1, then pinches the back of the experimental mouse and places the experimental mouse face down in the opposite clamping plate 11a2, so that the experimental mouse's limbs pass through the insertion slot 11a23 and are placed inside the adjustment slot 11a21.

[0072] Step 3, Flip and observe: Activate the drive source to drive the clamping plate 11a2 to rotate half a turn so that the back of the experimental mouse is facing down and the head is facing up. During the above process, the experimenter does not need to flip the wrist to fix the experimental mouse.

[0073] Step 4, Layer Fixation: The support plate 11a3 is lifted by the telescopic component 11a33. The experimenter controls the transmission handle 11a71 to rotate counterclockwise, so that the head pinching rod 11a31 and the torso pinching rod 11a32 on the inner side of the support plate 11a3 come into contact with the back of the experimental mouse and rotate in opposite directions. This causes the skin and muscles on the back of the experimental mouse to be rolled, restricted and fixed by the head pinching rod 11a31 and the torso pinching rod 11a32 rotating in opposite directions.

[0074] Step 5, assisted gavage: After the experimental mouse is fixed, the fixed component is used to tilt the head of the fixed experimental mouse upward. Then, with the support support 11a5 supporting the experimental personnel's arm, the gavage operation is completed on the experimental mouse with its head tilted upward under the premise that the experimental personnel's arm is supported.

[0075] Step 6, Examination and Diagnosis: After gavage or injection, the experimental mice are placed in either the first comparison chamber 12 or the second comparison chamber 13 for comparative observation. When the experimental mouse model needs to be dissected for diagnosis to examine the condition of the liver, the mouse can be fixed and rotated for dissection using the fixation component. After the dissection, the lifting platform 11b2 is moved closer to the weighing chamber by the moving component, so that the locking cover 11b1 on the side of the lifting platform 11b2 aligns with the top of the slot. Then, the lifting platform 11b2 is lowered, so that the locking cover 11b1 engages in the slot, and the transparent plate inside the locking cover 11b1 aligns with the top of the fixation component, allowing the diagnostic instrument 11c1 on the top of the locking cover 11b1 to examine and diagnose the liver of the experimental mouse through the transparent plate.

[0076] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A device for auxiliary culture and testing of a liver cancer animal model, comprising a housing (1), wherein the housing (1) includes a mouse culture and testing chamber (11), characterized in that, The culture and testing laboratory (11) includes: A fixing cavity (11a) includes a fixing assembly comprising a pair of parallel and opposite side plates (11a1), a flip-up clamping plate (11a2) vertically connected between the pair of side plates (11a1) for fixing a laboratory mouse, a horizontally inclined inclined plate frame (11a4) for supporting and fixing the side plates (11a1), a support plate (11a3) parallel to the clamping plate (11a2) longitudinally below it, and the support plate (11a3)... 3) A head pinching rod (11a31) and a torso pinching rod (11a32) for pinching and fixing the skin of the test mouse are installed on the upper part; opposing gear transmission mechanisms (11a8) with rounded corners are installed on the inner sides of the pair of side plates (11a1); the gear transmission mechanism (11a8) is vertically connected to the transmission rod (11a7) and the support plate (11a3); the support plate (11a3) has a vertically arranged part in the middle of its transverse direction for longitudinal displacement of the support plate (11a3) and... The experimental mouse's back contacts a telescopic component (11a33); a pair of side plates (11a1) have parallel moving slots (11a11) and positioning slots (11a12) extending through the middle of the longitudinal direction. A transmission rod (11a7) passes through the moving slot (11a11) and is connected to a transmission handle (11a71). A positioning rod (11a72) is rotatably connected to the transmission handle (11a71) and is inserted and fixed. The support plate (11a3) is lifted by the telescopic member (11a33). The experimenter controls the transmission handle (11a71) to rotate counterclockwise, so that the head pinch rod (11a31) and the torso pinch rod (11a32) on the inner side of the support plate (11a3) come into contact with the back of the experimental mouse and rotate in opposite directions, thereby making the skin and muscles on the back of the experimental mouse roll and restrict and fix them by the head pinch rod (11a31) and the torso pinch rod (11a32) rotating in opposite directions. Weighing chamber, which is connected to the fixed component via a movable component; and The test chamber (11c) includes a diagnostic instrument (11c1), through which a laboratory mouse is transferred longitudinally below the diagnostic instrument for test diagnosis.

2. The device for auxiliary culture and testing of liver cancer animal models according to claim 1, characterized in that, The fixed cavity (11a) is provided with a support bracket (11a5) on one side of the top edge for supporting the arm of the experimenter.

3. The device for auxiliary culture and testing of liver cancer animal models according to claim 1, characterized in that, A drive shaft (11a6) is connected between the clamping plate (11a2) and the side plate (11a1), and a rotating disk (11a61) is sleeved on the drive shaft (11a6).

4. The device for auxiliary culture and testing of liver cancer animal models according to claim 1, characterized in that, The clamping plate (11a2) is provided with an adjustment groove (11a21).

5. The device for auxiliary culture and testing of liver cancer animal models according to claim 1, characterized in that, The gear transmission mechanism (11a8) includes a driving member (11a81) and a driven member (11a82) that mesh with each other, and a linkage member (11a83) that is connected to the driving member (11a81) via a transmission belt.

6. The device for auxiliary culture and testing of liver cancer animal models according to claim 2, characterized in that, The moving assembly includes a locking cover (11b1) and a lifting platform (11b2) that is vertically fixed to one side of the locking cover (11b1). The lifting platform (11b2) is vertically mounted on a horizontal moving guide rail and moves horizontally.

7. The device for auxiliary culture and testing of liver cancer animal models according to claim 6, characterized in that, The locking cover (11b1) and the slot (11a9) are inserted and fixed together, and the slot (11a9) is located on one side of the fixing component.

8. The device for auxiliary culture and testing of liver cancer animal models according to claim 6, characterized in that, The inspection chamber (11c) also includes a disinfection component (11c2), which is evenly distributed at intervals along the top edge inside the locking cover (11b1) for disinfecting and sterilizing the fixing component.

9. The device for auxiliary culture and testing of liver cancer animal models according to claim 1, characterized in that, The housing (1) also includes a first comparison chamber (12) and a second comparison chamber (13).

10. The device for auxiliary culture and testing of liver cancer animal models according to claim 1, characterized in that, The outer casing (1) includes: Cleanroom (2), which is located on the outside of the box (1), is used to disinfect external equipment entering the culture and testing room (11); Waste liquid storage chamber (3) includes a liquid collection chamber (31) disposed on the outside side of the box body (1), and the liquid collection chamber (31) is connected to the cleaning chamber (2) and the fixed chamber (11a) through a conduit.

11. The device for auxiliary culture and testing of liver cancer animal models according to claim 10, characterized in that, The cleanroom (2) has a pull-out structure.

12. The device for auxiliary culture and testing of liver cancer animal models according to claim 1, characterized in that, An operating port (1e) is provided on one side of the box (1), and a hand protective device (1e1) is provided at the operating port (1e).

13. The device for auxiliary culture and testing of liver cancer animal models according to claim 1, characterized in that, The side wall of the box (1) is provided with an air vent (1d).

14. The device for auxiliary culture and testing of liver cancer animal models according to claim 1, characterized in that, The top of the box (1) is provided with an observation window (1a); the side wall of the box is provided with a box door (1b).

15. A method for auxiliary culture and testing of a liver cancer animal model, comprising the apparatus for auxiliary culture and testing of a liver cancer animal model as described in claim 6, characterized in that, The method includes the following steps: S1. Observation and culture: The experimental mice are placed inside the culture and testing chamber (11) for observation and culture before constructing the liver cancer model; S2, Back-facing fixation: When it is necessary to construct a liver cancer model by gavage and intraperitoneal injection on experimental mice, the experimenter fixes the experimental mouse to the clamping plate (11a2) by pinching the back of the experimental mouse. S3. Flip and observe: Start the drive source to drive the clamping plate (11a2) to flip half a turn so that the experimental mouse faces upward; S4. Layered Fixation: The support plate (11a3) is lifted by the telescopic member (11a33), so that the head pinching rod (11a31) and the torso pinching rod (11a32) on the inner side of the support plate (11a3) come into contact with the back of the experimental mouse and rotate in opposite directions, thereby causing the skin and muscles on the back of the experimental mouse to be rolled and fixed by the head pinching rod (11a31) and the torso pinching rod (11a32) rotating in opposite directions; S5. Assisted gavage: After the experimental mouse is fixed, the fixed component is used to tilt the head of the fixed experimental mouse upward. Then, with the support support (11a5) supporting the experimental personnel's arm, the gavage operation is completed on the experimental mouse with its head tilted upward under the premise that the experimental personnel's arm is supported. S6. Inspection and diagnosis: The fixing component is locked by the locking cover (11b1), and the diagnostic instrument (11c1) above the locking cover (11b1) in the longitudinal direction is used to inspect and diagnose the experimental mouse in the fixing component.

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