A liquid nitrogen storage device for visualizing cell liquid nitrogen recording

By introducing NFC reading cards and photosensitive development components into the liquid nitrogen storage device, the cumbersome problem of traditional cell freezing and recording is solved, and the effect of quickly obtaining cell information and improving cell bank management efficiency is achieved.

CN116391707BActive Publication Date: 2025-02-11SHANGHAI DUONING BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202310381082.8
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-04-11
Publication Date
2025-02-11
Estimated Expiration
2043-04-11

AI Technical Summary

Technical Problem

Traditional cell freezing records are complicated and it is difficult to efficiently locate the designated location, resulting in inconvenient cell bank management and use.

Method used

A liquid nitrogen storage device was designed. The NFC reading card on the surface of the tank corresponds to a cell culture dish at an equal height. Cell information is obtained through a mobile phone or reader with NFC function, and the cell distribution detection is achieved in combination with a photosensitive development component. .

Benefits of technology

It achieves rapid, convenient and intuitive acquisition of cell information, improves the management and use efficiency of cell databases, and through the use of photosensitive development components, it can significantly feedback the cell distribution and improves detection accuracy.

✦ Generated by Eureka AI based on patent content.

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Abstract

The present invention discloses a liquid nitrogen storage device for visualizing cell liquid nitrogen records, and belongs to the field of cell storage technology. A liquid nitrogen storage device for visualizing cell liquid nitrogen records comprises a liquid nitrogen storage tank and a cell culture dish, and also comprises a cell placement tube rack, which is arranged in a liquid nitrogen freezing chamber inside the liquid nitrogen storage tank, and a liquid nitrogen replenishing valve port is arranged on the surface of one side of the liquid nitrogen storage tank, and the liquid nitrogen replenishing valve port is connected with the liquid nitrogen freezing chamber. In order to solve the problem that it is inconvenient to check frozen cells due to the low temperature environment, the traditional paper version of the freezing and revival record is cumbersome, cannot be efficiently positioned to the designated position, and is inconvenient for the management and use of the cell bank, the NFC reader card on the surface of the tank body corresponds to the cell culture dish at the same height, and the cell information can be obtained conveniently, quickly and intuitively. When it is necessary to check the storage status of the cells, the current cell distribution can be obtained by using a mobile phone with NFC function or a reader close to the NFC reader card on the surface of the tank body.
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Description

Technical Field

[0001] The invention relates to the technical field of cell storage, in particular to a liquid nitrogen storage device for visualizing cell liquid nitrogen records. Background Art

[0002] The main experimental objects of cell biology research are various primary cultured or subcultured cells or cell lines. In order to avoid contamination caused by long-term in vitro culture of cultured cells or gene mutations caused by multiple subcultures, the experimenters freeze the cultured cells during the vigorous growth period. The frozen cells are stored in cell cryopreservation tubes with cell freezing solution, and then placed in liquid nitrogen at -196℃. In this ultra-low temperature environment, the cells can be preserved for a long time;

[0003] A Chinese patent with publication number CN213523621U discloses a cell storage cryogenic device, which facilitates the taking and placing of cells, avoids damage to the cell bottles caused by collisions between the cell bottles and the placement tanks, and can keep the chemical properties of liquid nitrogen from changing easily, making it difficult for cells to inactivate. When the cells are stored for a long time, the temperature of the cell storage cryogenic device can still be kept stable under the premise that the liquid nitrogen gradually evaporates.

[0004] In the above patent, the long-term storage of cells must use an ultra-low temperature liquid nitrogen storage environment. However, the low temperature environment is not convenient for checking frozen cells, and the traditional paper-based cryopreservation and resuscitation records are cumbersome and cannot be efficiently located at the designated location, which is inconvenient for the management and use of the cell bank. Therefore, it does not meet the existing needs, and a liquid nitrogen storage device with visual cell liquid nitrogen records is proposed. Summary of the invention

[0005] The purpose of the present invention is to provide a liquid nitrogen storage device with visualized cell liquid nitrogen records. The NFC reader card on the surface of the tank corresponds to the cell culture dish at the same height, so that the cell information can be obtained conveniently, quickly and intuitively. When the storage status of the cells needs to be checked, the current cell distribution can be obtained by bringing a mobile phone with NFC function or a reader close to the NFC reader card on the surface of the tank, which can solve the problems in the prior art.

[0006] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a liquid nitrogen storage device for visualizing cell liquid nitrogen recording, comprising a liquid nitrogen storage tank and a cell culture dish, and also comprising a cell placement tube rack, which is arranged in a liquid nitrogen freezing chamber inside the liquid nitrogen storage tank, a liquid nitrogen replenishing valve port is arranged on the surface of one side of the liquid nitrogen storage tank, and the liquid nitrogen replenishing valve port is connected with the liquid nitrogen freezing chamber, a plurality of NFC reading cards are arranged on the surface of the other side of the liquid nitrogen storage tank, and the NFC reading cards extend to the inner side of the liquid nitrogen freezing chamber, a lifting tank cover is arranged on the top of the liquid nitrogen storage tank, one end of the cell placement tube rack is connected to the lifting tank cover by an internal thread, and a positioning power supply base is arranged on the bottom of the liquid nitrogen freezing chamber, and the positioning power supply base extends to the outer side of the liquid nitrogen storage tank.

[0007] Preferably, the threaded contact at the bottom of the cell placement tube rack is electrically connected to the positioning power supply base, a plurality of vessel grooves are provided inside the cell placement tube rack, the cell culture dish is connected to the cell placement tube rack through the vessel grooves, a telescopic rubber block is provided on one side of the vessel groove, and the telescopic rubber block is slidably connected to the cell placement tube rack.

[0008] Preferably, a pop-up switch is provided on the outer surface of the cell placement tube rack, and the horizontal height of the pop-up switch is the same as that of the NFC reader card. A photosensitive developing assembly is provided inside the vessel groove, and the photosensitive developing assembly includes a ring-arranged light board and a photosensitive plate.

[0009] Preferably, the ring-arranged light board is located below the cell culture dish, a light-transmitting lens is arranged between the ring-arranged light board and the cell culture dish, LED dynamic lamp beads are arranged on the outer surface of the ring-arranged light board, the LED dynamic lamp beads are distributed in a ring shape, the LED dynamic lamp beads are electrically connected to the ring-arranged light board through the metal lamp holder at the bottom, and the LED dynamic lamp beads are rotatably connected to the metal lamp holder.

[0010] Preferably, the photosensitive plate is located above the cell culture dish, a polarizing lens is arranged between the photosensitive plate and the cell culture dish, an electromagnetic turntable is arranged above the photosensitive plate, the electromagnetic turntable is rotatably connected to the cell placement tube rack, and the annular sleeve on the outer side of the electromagnetic turntable is fit-connected to the photosensitive plate.

[0011] Preferably, a magnetic axis is arranged inside the annular sleeve, and the magnetic axis is arranged in a fan-shaped structure. The magnetic axis rotates along the inside of the annular sleeve following the electromagnetic turntable.

[0012] Preferably, a touch sensing groove is provided on the other side of the vessel groove, and a pop-up switch is installed inside the touch sensing groove. The pop-up switch is configured as a hollow structure, and an integrally formed abutment shaft is provided at one end of the pop-up switch. The abutment shaft extends to the interior of the vessel groove, and the abutment shaft is fitly connected to the cell culture dish.

[0013] Preferably, a pressing spring is provided on both sides of the abutment shaft, one end of the pressing spring is fitted with the contact sheet sensing slot, and the other end of the pressing spring is fitted with the pop-up switch, and the pop-up switch is telescopically connected with the contact sheet sensing slot through the pressing spring.

[0014] Preferably, an NFC writing module is provided on the inner surface of the pop-up switch, two ends of the NFC writing module respectively extend to the inside of the contact sheet sensing slot, and the NFC writing module is electrically connected to the photosensitive board.

[0015] Preferably, a rubber insulating layer is provided on the outer surface of the pop-up switch, and a metal contact is provided between the contact sensing slot and the NFC writing module.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] 1. In the present invention, a cell culture dish containing cells is placed in a cell placement tube rack. Each cell culture dish has an independent placement space. The NFC reader card on the surface of the tank corresponds to the cell culture dish at the same height, so that the cell information can be obtained conveniently, quickly and intuitively. When the storage status of the cells needs to be checked, the current cell distribution can be obtained by placing a mobile phone with NFC function or a reader close to the NFC reader card on the surface of the tank;

[0018] 2. In the present invention, the photosensitive developing assembly is divided into two unit structures, namely, a ring-arranged light board and a photosensitive plate. The ring-arranged light board is installed at the bottom of the vessel groove, and a plurality of LED dynamic lamp beads are distributed on its surface. When it is necessary to observe the cell distribution, the NFC reader card will trigger the LED dynamic lamp bead switch, and the light source of the lamp bead can be irradiated above the cell culture dish, so that the area with cell distribution and the area without cell distribution will form obvious light and dark partitions, which will then be fed back on the surface of the photosensitive plate to achieve the purpose of cell distribution detection;

[0019] 3. In the process of light source detection, the electromagnetic turntable on the top of the photosensitive plate will be energized to rotate at a uniform speed, and the rotation of the electromagnetic turntable will drive the magnetic axis inside the annular sleeve to rotate synchronously. At this time, under the influence of magnetic force, the LED dynamic lamp beads can cooperate with the metal lamp holder to realize dynamic adjustment operation. The range of dynamic adjustment is that the LED dynamic lamp beads on the outer circle of the lamp board follow the moving direction of the magnetic axis to perform angular deflection, while the LED dynamic lamp beads in the center area are still affected by the magnetic force of the electromagnetic turntable to maintain a vertical upward irradiation angle. In this way, the cells in the vessel can be irradiated from different angles by the peripheral lamp beads, thereby improving the feedback accuracy of light and dark areas. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is the overall front view of the present invention;

[0021] Figure 2 It is an overall side view of the present invention;

[0022] Figure 3 It is a schematic diagram of the overall cross-sectional structure of the present invention;

[0023] Figure 4 This is a schematic diagram of the cell placement rack structure of the present invention;

[0024] Figure 5 It is a schematic diagram of the structure of the cell culture dish of the present invention;

[0025] Figure 6 It is a schematic structural diagram of a photosensitive developing assembly of the present invention;

[0026] Figure 7 is a schematic cross-sectional structure diagram of a photosensitive developing assembly of the present invention;

[0027] Figure 8 This is a schematic diagram of the structure of the LED dynamic lamp bead of the present invention;

[0028] Fig. 9 It is a schematic diagram of the cross-sectional structure of the vessel groove of the present invention;

[0029] Fig.10 It is a schematic diagram of the pop-up switch structure of the present invention.

[0030] In the figure: 1. liquid nitrogen storage tank; 2. cell placement tube rack; 3. cell culture dish; 101. liquid nitrogen replenishing valve port; 102. lifting tank cover; 103. NFC reader card; 104. liquid nitrogen freezing chamber; 105. positioning power base; 201. vessel slot; 202. photosensitive developing assembly; 203. threaded contact; 204. telescopic rubber block; 205. pop-up switch; 206. contact sensing slot; 2021. ring light board; 2022. light-transmitting lens; 2023. photosensitive board; 2024. polarized lens; 2025. ring sleeve; 2026. electromagnetic turntable; 2027. magnetic axis; 2028. LED dynamic lamp beads; 2029. metal lamp holder; 2051. NFC writing module; 2052. anti-axis; 2053. pressing spring. DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] See also Figure 1-3, an embodiment provided by the present invention: a liquid nitrogen storage device for visualizing cell liquid nitrogen recording, comprising a liquid nitrogen storage tank 1 and a cell culture dish 3, and also comprising a cell placement tube rack 2, which is arranged in a liquid nitrogen freezing chamber 104 inside the liquid nitrogen storage tank 1, a liquid nitrogen replenishing valve port 101 is arranged on the surface of one side of the liquid nitrogen storage tank 1, and the liquid nitrogen replenishing valve port 101 is connected with the liquid nitrogen freezing chamber 104, a plurality of NFC reading cards 103 are arranged on the surface of the other side of the liquid nitrogen storage tank 1, and the NFC reading cards 103 extend to the inner side of the liquid nitrogen freezing chamber 104, a lifting tank cover 102 is arranged on the top of the liquid nitrogen storage tank 1, one end of the cell placement tube rack 2 is connected to the lifting tank cover 102 through an internal thread, and a positioning power supply base 105 is arranged at the bottom of the liquid nitrogen freezing chamber 104, and the positioning power supply base 105 extends to the outer side of the liquid nitrogen storage tank 1;

[0033] The cell culture dish 3 containing cells is placed into the cell placement tube rack 2, each cell culture dish 3 has an independent placement space, and then the lifting tank cover 102 is screwed and connected to the top of the cell placement tube rack 2, and the cell placement tube rack 2 is inserted into the liquid nitrogen freezing chamber 104 inside the liquid nitrogen storage tank 1 through the lifting tank cover 102. In the process of inserting the cell placement tube rack 2, it is necessary to connect and fix its bottom with the positioning power base 105 inside the cavity, and the positioning power base 105 is connected to the external power supply through a plug-in line, and the positioning power base 105 is used to power the electronic components inside the cell placement tube rack 2. When it is necessary to check the storage status of the cells, the current cell distribution status can be obtained through a mobile phone with NFC function or a reader close to the NFC reading card 103 on the surface of the tank. Each NFC reading card 103 corresponds to a cell culture dish 3 at an equal height, so that cell information can be obtained conveniently, quickly and intuitively.

[0034] See also Figure 4-5 , the threaded contact 203 at the bottom of the cell placement tube rack 2 is electrically connected to the positioning power supply base 105, a plurality of vessel grooves 201 are arranged inside the cell placement tube rack 2, the cell culture dish 3 is connected to the cell placement tube rack 2 through the vessel grooves 201, a telescopic rubber block 204 is arranged on one side of the vessel groove 201, the telescopic rubber block 204 is slidably connected to the cell placement tube rack 2, a pop-up switch 205 is arranged on the outer surface of the cell placement tube rack 2, the horizontal height of the pop-up switch 205 is the same as that of the NFC reader card 103, and a photosensitive developing component 202 is arranged inside the vessel groove 201;

[0035] The vessel groove 201 is arranged vertically inside the cell placement tube rack 2. The cell culture dish 3 can be first locked in the opening area of ​​the vessel groove 201, and then the cell culture dish 3 can be pushed into the vessel groove 201, and the cell culture dish 3 can be fixed with the telescopic rubber block 204 at the opening to prevent the cell culture dish 3 from falling during the movement and insertion and removal of the cell placement tube rack 2, thereby improving the installation stability of the cell culture dish 3. When the cell culture dish 3 needs to be taken out, the cell culture dish 3 inside the vessel groove 201 can be pushed out by pressing the pop-up switch 205 on the back.

[0036] See also Figure 6-8 The photosensitive developing assembly 202 includes a ring-shaped light board 2021 and a photosensitive plate 2023. The ring-shaped light board 2021 is located below the cell culture dish 3. A light-transmitting lens 2022 is arranged between the ring-shaped light board 2021 and the cell culture dish 3. The outer surface of the ring-shaped light board 2021 is provided with LED dynamic lamp beads 2028. The LED dynamic lamp beads 2028 are distributed in a ring shape. The LED dynamic lamp beads 2028 are electrically connected to the ring-shaped light board 2021 through a metal lamp holder 2029 at the bottom. The LED dynamic lamp beads 2028 are rotatably connected to the metal lamp holder 2029. The plate 2023 is located above the cell culture dish 3, a polarizing lens 2024 is arranged between the photosensitive plate 2023 and the cell culture dish 3, an electromagnetic turntable 2026 is arranged above the photosensitive plate 2023, the electromagnetic turntable 2026 is rotatably connected to the cell placement tube rack 2, an annular sleeve 2025 outside the electromagnetic turntable 2026 is fitted and connected to the photosensitive plate 2023, a magnetic axis 2027 is arranged inside the annular sleeve 2025, the magnetic axis 2027 is arranged in a fan-shaped structure, and the magnetic axis 2027 rotates along the inside of the annular sleeve 2025 following the electromagnetic turntable 2026;

[0037] The photosensitive developing assembly 202 is installed inside the vessel groove 201, and is divided into two unit structures, namely, a ring-shaped light board 2021 and a photosensitive plate 2023. The ring-shaped light board 2021 is installed at the bottom of the vessel groove 201, and a plurality of LED dynamic lamp beads 2028 are distributed on its surface. The bottom of the LED dynamic lamp bead 2028 is a spherical structure, and can be rotated and adjusted at a small angle in conjunction with the metal lamp holder 2029 at the bottom. The photosensitive plate 2023 is at the top of the vessel groove 201. When it is necessary to observe the cell distribution, the NFC reader card 103 will trigger the switch of the LED dynamic lamp bead 2028, and the light source of the lamp bead can be irradiated above the cell culture dish 3, so that the area with cell distribution and the area without cell distribution will form obvious light and dark partitions, which will then be fed back on the surface of the photosensitive plate 2023 to achieve the purpose of cell distribution detection.

[0038] During the light source detection process, the electromagnetic turntable 2026 on the top of the photosensitive plate 2023 will be energized to rotate at a uniform speed, and the rotation of the electromagnetic turntable 2026 will drive the magnetic axis 2027 inside the annular sleeve 2025 to rotate synchronously. At this time, under the influence of magnetic force, the LED dynamic lamp beads 2028 can cooperate with the metal lamp holder 2029 to achieve dynamic adjustment operation. The dynamic adjustment range is that the LED dynamic lamp beads 2028 on the outer circle of the light board follow the moving direction of the magnetic axis 2027 to deflect the angle, while the LED dynamic lamp beads 2028 in the center area are still affected by the magnetic force of the electromagnetic turntable 2026. The angle of illumination is still maintained vertically upward, so that the cells in the vessel can be illuminated from different angles through the outer lamp beads, thereby improving the feedback accuracy of the light and dark areas.

[0039] See also Figure 9-10 A touch sensing groove 206 is provided on the other side of the vessel groove 201, and a pop-up switch 205 is installed inside the touch sensing groove 206. The pop-up switch 205 is set as a hollow structure. An integrally formed abutment shaft 2052 is provided at one end of the pop-up switch 205. The abutment shaft 2052 extends to the inside of the vessel groove 201. The abutment shaft 2052 is closely connected to the cell culture dish 3. Pressing springs 2053 are provided on both sides of the abutment shaft 2052. One end of the pressing spring 2053 is closely connected to the touch sensing groove 206. The pressing spring 2053 The other end of the pop-up switch 205 is in contact with the pop-up switch 205, and the pop-up switch 205 is telescopically connected to the contact sheet sensing slot 206 through the pressing spring 2053. The inner surface of the pop-up switch 205 is provided with an NFC writing module 2051, and the two ends of the NFC writing module 2051 extend to the inside of the contact sheet sensing slot 206 respectively. The NFC writing module 2051 is electrically connected to the photosensitive plate 2023, and the outer surface of the pop-up switch 205 is provided with a rubber insulating layer, and a metal contact sheet is provided between the contact sheet sensing slot 206 and the NFC writing module 2051;

[0040] The NFC writing module 2051 is attached to the inner surface of the pop-up switch 205. When the spring 2053 is pressed to push the pop-up switch 205 out, the NFC writing module 2051 will contact the contact sensing groove 206, and the distance between the NFC writing module 2051 and the NFC reading card 103 is shortened, which is conducive to the establishment of the data channel. The data information detected by the photosensitive structure can be transmitted to the external NFC reading card 103 through the NFC writing module 2051, so that the reading operation can be realized.

[0041] Working principle: put the cell culture dish 3 containing cells into the cell placement tube rack 2, then tighten the lifting tank cover 102 and the top of the cell placement tube rack 2, insert the cell placement tube rack 2 into the liquid nitrogen freezing chamber 104 inside the liquid nitrogen storage tank 1 through the lifting tank cover 102, and in the process of inserting the cell placement tube rack 2, it is necessary to connect and fix its bottom with the positioning power base 105 inside the cavity, and the positioning power base 105 is connected to the external power supply through a plug-in line, and the positioning power base 105 is used to power the electronic components inside the cell placement tube rack 2, and each cell culture dish 3 has an independent placement space, and the vessel groove 201 is arranged vertically inside the cell placement tube rack 2, and the cell culture dish 3 can be first clamped in the opening area of ​​the vessel groove 201, and then the cell culture dish 3 can be pushed into the vessel groove 201, and the cell culture dish 3 is fixed with the telescopic rubber block 204 at the opening to avoid the cell culture dish 3 from falling during the process of moving and plugging in the cell placement tube rack 2. To improve the installation stability of the cell culture dish 3, when the cell culture dish 3 needs to be taken out, the cell culture dish 3 inside the dish groove 201 can be pushed out by pressing the pop-up switch 205 on the back, and the NFC writing module 2051 is attached to the inner surface of the pop-up switch 205. When the spring 2053 is pressed to push the pop-up switch 205 out, the NFC writing module 2051 will contact the contact sensing groove 206, and the distance between the NFC writing module 2051 and the NFC reading card 103 is shortened, which is conducive to the establishment of the data channel. The data information detected by the photosensitive structure can be transmitted to the NFC reading card 103 on the outside through the NFC writing module 2051, so that the reading operation can be realized. When it is necessary to check the storage status of the cells, the current cell distribution can be obtained by using a mobile phone with NFC function or a reader close to the NFC reading card 103 on the surface of the tank. Each NFC reading card 103 corresponds to a cell culture dish 3 at the same height, so that the cell information can be obtained conveniently, quickly and intuitively.

[0042] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0043] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A liquid nitrogen storage device for visualizing cell liquid nitrogen recording, comprising a liquid nitrogen storage tank (1) and a cell culture dish (3), characterized in that ; It also comprises a cell placement tube rack (2), which is arranged in a liquid nitrogen freezing chamber (104) inside the liquid nitrogen storage tank (1); a liquid nitrogen replenishing valve port (101) is arranged on the surface of one side of the liquid nitrogen storage tank (1); the liquid nitrogen replenishing valve port (101) is connected to the liquid nitrogen freezing chamber (104); a plurality of NFC reading cards (103) are arranged on the surface of the other side of the liquid nitrogen storage tank (1); the NFC reading cards (103) extend to the inside of the liquid nitrogen freezing chamber (104); a lifting tank cover (102) is arranged on the top of the liquid nitrogen storage tank (1); one end of the cell placement tube rack (2) is connected to the lifting tank cover (102) via an internal thread; a positioning power supply base (105) is arranged on the bottom of the liquid nitrogen freezing chamber (104); the positioning power supply base (105) extends to the outside of the liquid nitrogen storage tank (1); The outer surface of the cell placement tube rack (2) is provided with a pop-up switch (205), and the horizontal height of the pop-up switch (205) is the same as that of the NFC reading card (103). The threaded contact (203) at the bottom of the cell placement tube rack (2) is electrically connected to the positioning power supply base (105). The interior of the cell placement tube rack (2) is provided with a plurality of vessel grooves (201), and the cell culture dish (3) is connected to the cell placement tube rack (2) through the vessel grooves (201). A telescopic rubber block (204) is provided on one side of the vessel groove (201), and the telescopic rubber block (204) is slidably connected to the cell placement tube rack (2). A photosensitive developing assembly (202) is provided inside the vessel groove (201), and the photosensitive developing assembly (202) comprises a ring-shaped light board (2021) and a photosensitive plate (2023); A touch-sheet sensing groove (206) is provided on the other side of the vessel groove (201); a pop-up switch (205) is installed inside the touch-sheet sensing groove (206); the pop-up switch (205) is configured as a hollow structure; an integrally formed abutment shaft (2052) is provided at one end of the pop-up switch (205); the abutment shaft (2052) extends into the interior of the vessel groove (201); and the abutment shaft (2052) is fitted and connected to the cell culture dish (3); A pressing spring (2053) is provided on both sides of the abutment shaft (2052), one end of the pressing spring (2053) is fitted with the contact sheet sensing slot (206), and the other end of the pressing spring (2053) is fitted with the pop-up switch (205), and the pop-up switch (205) is telescopically connected to the contact sheet sensing slot (206) via the pressing spring (2053); An NFC writing module (2051) is provided on the inner surface of the pop-up switch (205), two ends of the NFC writing module (2051) respectively extend into the interior of the contact sheet sensing slot (206), the NFC writing module (2051) is electrically connected to the photosensitive plate (2023), a rubber insulating layer is provided on the outer surface of the pop-up switch (205), and a metal contact sheet is provided between the contact sheet sensing slot (206) and the NFC writing module (2051).

2. A liquid nitrogen storage device for visualizing cell liquid nitrogen recording according to claim 1, characterized in that: The annular light board (2021) is located below the cell culture dish (3); a light-transmitting lens (2022) is provided between the annular light board (2021) and the cell culture dish (3); LED dynamic lamp beads (2028) are provided on the outer surface of the annular light board (2021); the LED dynamic lamp beads (2028) are distributed in an annular shape; the LED dynamic lamp beads (2028) are electrically connected to the annular light board (2021) via a metal lamp holder (2029) at the bottom; and the LED dynamic lamp beads (2028) are rotatably connected to the metal lamp holder (2029).

3. A liquid nitrogen storage device for visualizing cell liquid nitrogen recording according to claim 2, characterized in that: The photosensitive plate (2023) is located above the cell culture dish (3); a polarized lens (2024) is provided between the photosensitive plate (2023) and the cell culture dish (3); an electromagnetic turntable (2026) is provided above the photosensitive plate (2023); the electromagnetic turntable (2026) is rotatably connected to the cell placement tube rack (2); and an annular sleeve (2025) on the outside of the electromagnetic turntable (2026) is fitted and connected to the photosensitive plate (2023).

4. A liquid nitrogen storage device for visualizing cell liquid nitrogen recording according to claim 3, characterized in that: A magnetic axis (2027) is arranged inside the annular sleeve (2025), and the magnetic axis (2027) is arranged in a fan-shaped structure. The magnetic axis (2027) rotates along the inside of the annular sleeve (2025) following the electromagnetic rotating disk (2026).

Citation Information

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