A bionic blood with adjustable viscosity and its preparation method

Abstract

The present invention relates to the field of bionic blood technology, and discloses a preparation method of a bionic blood with adjustable viscosity, comprising the following steps: (1) preparing an aqueous glycerol solution by mixing glycerol and pure water at a weight percentage of 24-28%:72-76%; (2) mixing the aqueous glycerol solution and propylene glycol at a weight percentage of 31.2-100%:0-68.8%, slowly stirring in a water bath at 40-70°C for 2 min and then standing for 15 min; (3) adding sodium chloride accounting for about 0-0.9% by weight to the mixture obtained in step (2), slowly stirring in a water bath at 40-70°C for 5 min and then standing for 1 h until the sodium chloride is gradually completely dissolved and there are no bubbles and no floccules, obtaining a colorless transparent liquid. The bionic blood in the present invention is simple to prepare, the solution is stable, the flow performance is good, and the density value is stable and the viscosity value is adjustable, which can meet the requirements for conventional bionic blood; it can be better applied to the research on the interaction between bionic devices and the blood circulation system, and provide more accurate guidance for the research and development of human bionic organs and the control of physiological control strategies in clinical practice.

Description

Technical Field

[0001] The present invention relates to the technical field of bionic blood, and specifically relates to a bionic blood with adjustable viscosity and a preparation method thereof. Background Art

[0002] The human blood circulation system is a complex system with highly dynamic characteristics. In the early stage of the research on the human blood circulation system, the main research methods were animal experiments, human experiments, and in vitro simulation experiments. Animal experiments and human experiments are often restricted by technical conditions and social ethics. Conducting systematic modeling and in vitro simulation experiments can avoid the above risks, save a large amount of time and research costs, and in vitro simulation experiments have always been an effective tool for researching, evaluating, and verifying the design and performance of bionic organs.

[0003] Bionic blood is an important liquid medium for in vitro simulation experiments, and its density value and viscosity value are often required to conform to blood characteristics. The density value and viscosity value are important input parameters in systematic modeling such as Matlab and CFD. Blood is mainly composed of blood cells and plasma, and is a non-Newtonian fluid with a viscosity value that is not a constant, that is, it will exhibit different viscosities at different shear rates, which makes blood flow much more complex than ordinary fluids. The density value of adult blood is relatively fixed, and the relative density to water is 1.050 - 1.060. Due to different blood vessel diameters, lengths, and the influence of blood pressure, the apparent viscosity value of blood in blood vessels is different. During the blood sample detection process, the tester uses a blood viscometer to measure the viscosity of adult blood at different shear rates of (1 - 200) s -1 The normal values at high, medium, and low shear rates are in the range of (0.1 - 20) mPa·s, and the conventional range of the apparent viscosity of blood flowing in blood vessels is mostly (1 - 15) mPa·s.

[0004] In in vitro simulation experiments, the optimal relative density of bionic blood is 1.050 - 1.060, and the viscosity value is in the range of (0.1 - 20) mPa·s. Currently, the liquid media applied to in vitro experiments mainly include glycerol aqueous solution, glycerol, physiological saline solution, silicone oil, etc. As described in the literature, Zhao Yiming et al. prepared a working fluid with a density of 1.06×10 3 kg / m 3 and a viscosity of 2.5 mPa·s similar to human blood by mixing water and glycerol at a ratio of 2.3:1; Luo Meihong et al. mixed 40% by volume of glycerol and 60% by volume of water, and added sodium chloride accounting for about 7% by weight to the mixture. The density of this working solution is the same as that of blood at 37°C, which is 1.056×10 3 kg / m 3close and with viscosity within the normal blood viscosity range; Patent CN 114190898 discloses the use of silicone oil as a liquid medium; Mejia J et al. used physiological saline as the working fluid, and Foad K et al. used PBS solution as the working fluid for the in vitro blood flow characteristics test platform; some scientists also used PSP solution; some scholars used Ringer's solution. The above bionic blood has the characteristics of simple preparation, stable solution, and good fluidity. Its disadvantage is that the viscosity value is fixed and single. Once the preparation ratio is adjusted, the viscosity value can be changed, but the density value is no longer within the blood density range, and it cannot well characterize the blood characteristics, so its application range is limited. Summary of the Invention

[0005] Technical problems to be solved

[0006] In view of the deficiencies of the prior art, the present invention provides an adjustable-viscosity bionic blood and its preparation method, and develops an adjustable-viscosity bionic blood with simple preparation, stable solution, good fluidity, stable density value, and adjustable viscosity value, that is, the relative density is 1.050 - 1.060, and the viscosity value is adjusted to vary between (1 - 15) mPa·s by the preparation ratio, and this range can meet the requirements for conventional bionic blood. It can be better applied to the research on the interaction between bionic devices and the blood circulation system, and provide more accurate guidance for the research and development of human bionic organs and the control of physiological control strategies in clinical practice.

[0007] Technical solutions

[0008] To achieve the above object, the present invention provides the following technical solutions:

[0009] An adjustable-viscosity bionic blood, the components of the bionic blood include glycerol, pure water, propylene glycol, and sodium chloride, and glycerol, pure water, propylene glycol, and sodium chloride are prepared in a certain proportion in sequence.

[0010] A preparation method for an adjustable-viscosity bionic blood, comprising the following steps:

[0011] (1) Prepare an aqueous glycerol solution by mixing glycerol and pure water in a weight percentage of 24 - 28%:72 - 76%;

[0012] (2) Mix the aqueous glycerol solution obtained in step (1) and propylene glycol in a weight percentage of 31.2 - 100%:0 - 68.8%, and slowly stir in a water bath at 40 - 70°C for 2 min and then let it stand for 15 min;

[0013] (3) Add sodium chloride with a weight of about 0 - 0.9% to the mixture obtained in step (2), slowly stir in a water bath at 40 - 70°C for 5 min and then let it stand for 1 h until the sodium chloride is gradually completely dissolved and there are no bubbles and no flocs, and a colorless transparent liquid is obtained, which is the bionic blood.

[0014] When two different liquids are mixed, the density (ρ) of the mixed liquid conforms to the principle of returning to the middle: ρ = (m1 + m2) / (m1 / ρ1 + m2 / ρ2)

[0015] Where: m1 and m2 are the masses of the two known liquids; ρ1 and ρ2 are the densities of the two known liquids;

[0016] Through calculation, the mass ratio of glycerol and pure water is initially selected, and a vibrating densitometer is further used to measure and determine the density value of the mixed liquid.

[0017] On this basis, a glycerol aqueous solution with a relative density of 1.050 - 1.060 is prepared by adjusting the mass ratio of glycerol and pure water. At this time, the viscosity of the mixed liquid is at the lowest value. Through experiments, the weight percentages of glycerol and pure water required for the glycerol aqueous solution can be arbitrarily 24 - 28%: 72 - 76%, can be 26%: 74%, at this time the relative density is 1.055; the maximum is 28%: 72%, at this time the relative density is 1.060; the minimum is 24%: 76%, at this time the relative density is 1.050.

[0018] Then, propylene glycol with a density equivalent to that of blood and a viscosity greater than that of blood is added. The glycerol aqueous solution and propylene glycol are mixed according to the weight percentage of 31.2 - 100%: 0 - 68.8% as needed. The more propylene glycol is added, the greater the viscosity value of the mixed liquid, and the viscosity of the mixed liquid can be increased to the target value.

[0019] When preparing a mixed liquid with the required viscosity (v3) using two liquids with known viscosities (v1, v2) and densities (ρ1, ρ2), the viscosity of the mixed liquid conforms to the following law:

[0020]

[0021] Where: M1 and M2 are the mass percentages of the two known liquids;

[0022] Finally, sodium chloride with a weight accounting for about 0 - 0.9% can be added to the above mixed liquid to increase the density of the mixed liquid to the original density value. Adding propylene glycol to the glycerol aqueous solution increases the viscosity of the mixed liquid and will slightly reduce the density of the mixed liquid; adding sodium chloride helps to increase the density of the mixed liquid and has little effect on the viscosity.

[0023] Beneficial effects

[0024] Compared with the prior art, the present invention provides a bionic blood with adjustable viscosity and its preparation method, having the following beneficial effects:

[0025] The bionic blood in the present invention is simple to prepare, has a stable solution, good flow performance, a stable density value, and an adjustable viscosity value, and can meet the requirements for conventional bionic blood; it can be better applied to the research on the interaction between bionic devices and the blood circulation system, and provide more accurate guidance for the research and development of bionic organs and physiological control strategies in clinical practice. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 Shows the density and viscosity of an aqueous glycerol solution at different weight percentages at 37°C in the present invention;

[0027] Figure 2 Shows the density and viscosity of an aqueous glycerol solution with a relative density of 1.050 - 1.060 at different weight percentages at 37°C in the present invention;

[0028] Figure 3 Shows the density and viscosity of a 26%:74% aqueous glycerol solution and propylene glycol at different mass percentages in the present invention;

[0029] Figure 4 Shows the density and viscosity of a 26%:74% aqueous glycerol solution and propylene glycol at different weight percentages with a small amount of NaCl added in the present invention;

[0030] Figure 5 Shows the density and viscosity of a 24%:76% aqueous glycerol solution and propylene glycol at different weight percentages in the present invention;

[0031] Figure 6 Shows the density and viscosity of a 24%:76% aqueous glycerol solution and propylene glycol at different weight percentages with a small amount of NaCl added in the present invention;

[0032] Figure 7 Shows the density and viscosity of a 28%:72% aqueous glycerol solution and propylene glycol at different weight percentages in the present invention;

[0033] Figure 8 Shows the density and viscosity of a 28%:72% aqueous glycerol solution and propylene glycol at different weight percentages with a small amount of NaCl added in the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0034] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.

[0035] Embodiment 1

[0036] Please refer to Figures 1-5 , a bionic blood with adjustable viscosity, the components of the bionic blood include glycerol, pure water, propylene glycol, and sodium chloride, and glycerol, pure water, propylene glycol, and sodium chloride are prepared in a certain proportion in sequence.

[0037] The preparation method of the bionic blood with adjustable viscosity includes the following steps:

[0038] Step (1): Prepare an aqueous glycerol solution by mixing glycerol and pure water at a weight percentage of 24-28%:72-76%.

[0039] Step (2): Mix the aqueous glycerol solution obtained in step (1) and propylene glycol at a weight percentage of 31.2-100%:0-68.8%, slowly stir in a water bath at 40-70°C for 2 min, and then let it stand for 15 min.

[0040] Step (3): Add sodium chloride with a weight of about 0-0.9% to the mixture obtained in step (2), slowly stir in a water bath at 40-70°C for 5 min, and then let it stand for 1 h until the sodium chloride is gradually completely dissolved and there are no bubbles or flocs, obtaining a colorless transparent liquid, which is the bionic blood.

[0041] When two different liquids are mixed, the density (ρ) of the mixture conforms to the principle of convergence: ρ=(m1 + m2) / (m 1 / ρ1 + m 2 / ρ2)

[0042] Where: m1 and m2 are the masses of two known liquids; ρ1 and ρ2 are the densities of two known liquids.

[0043] Through calculation, initially select the mass ratio of glycerol and pure water, and further measure and determine the density value of the mixture using a vibrating densitometer.

[0044] On this basis, adjust the mass ratio of glycerol and pure water to prepare an aqueous glycerol solution with a relative density of 1.050-1.060, and at this time the viscosity of the mixture is the lowest value. Through experiments, the weight percentages of glycerol and pure water required for the aqueous glycerol solution can be arbitrarily 24-28%:72-76%, can be 26%:74%, and at this time the relative density is 1.055; the maximum is 28%:72%, and at this time the relative density is 1.060; the minimum is 24%:76%, and at this time the relative density is 1.050.

[0045] Then add propylene glycol with a density equivalent to that of blood and a viscosity greater than that of blood. The aqueous glycerol solution and propylene glycol are mixed according to the required weight percentage of 31.2-100%:0-68.8%. The more the mass of propylene glycol added, the greater the viscosity value of the mixture, and the viscosity of the mixture can be increased to the target value.

[0046] When preparing a mixture with the required viscosity (v3) using two liquids with known viscosities (v1, v2) and densities (ρ1, ρ2), the viscosity of the mixture conforms to the following law:

[0047]

[0048] Wherein: M1 and M2 are mass percentages of two known liquids.

[0049] Finally, sodium chloride with a weight of about 0 - 0.9% can be added to the above-mentioned mixture to increase the density of the mixture to the original density value. After adding propylene glycol to the glycerol aqueous solution, the viscosity of the mixture increases, and the density of the mixture will decrease slightly; after adding sodium chloride, it helps to increase the density of the mixture and has little effect on the viscosity.

[0050] Experiment 1

[0051] A preparation method of a viscosity-adjustable bionic blood, comprising the following steps:

[0052] Step (1): Prepare a glycerol aqueous solution by mixing glycerol and pure water at a weight percentage of 26%:74%.

[0053] Step (2): Mix the glycerol aqueous solution and propylene glycol with weight percentages of 51%, 56.5%, 61%, 64.5%, 67.5%, and 68.8% respectively by weight percentage, and slowly stir in a water bath at 40 - 70°C for 2 min and then let it stand for 15 min; its density and viscosity are as Figure 3 shown;

[0054] Step (3): Add sodium chloride with weights of 0.1%, 0.2%, 0.3%, 0.4%, 0.6%, and 0.8% respectively to the mixture obtained in step (2), slowly stir in a water bath at 40 - 70°C for 5 min and then let it stand for 1 h until the sodium chloride is gradually completely dissolved and there are no bubbles and no flocculants, obtaining a colorless and transparent liquid, which is the bionic blood; its density and viscosity are as Figure 4 shown;

[0055] The component weight percentages are shown in Table 1:

[0056]

[0057]

[0058] Table 1: Weight percentages of NaCl added to the mixture of 26%:74% glycerol aqueous solution and propylene glycol.

[0059] Experiment 2

[0060] A preparation method of a viscosity-adjustable bionic blood, comprising the following steps:

[0061] Step (1): Prepare a glycerol aqueous solution by mixing glycerol and pure water at a weight percentage of 24%:76%.

[0062] Step (2): Mix the glycerol aqueous solution and propylene glycol with weight percentages of 56.5%, 61%, 64.5%, 67.5%, and 68.8% respectively by weight percentage, slowly stir in a water bath at 40 - 70 °C for 2 min, and then let it stand for 15 min; its density and viscosity are as Figure 5 shown;

[0063] Step (3): Add sodium chloride with weights of 0.2%, 0.3%, 0.4%, 0.6%, and 0.9% respectively to the mixed solution obtained in step (2), slowly stir in a water bath at 40 - 70 °C for 5 min, and then let it stand for 1 h until the sodium chloride is gradually completely dissolved, with no bubbles and no floccules present, to obtain a colorless transparent liquid, which is the said bionic blood; its density and viscosity are as Figure 6 shown;

[0064] The component weight percentages are as shown in Table 2:

[0065]

[0066] Table 2: Weight percentages of NaCl added to the 24%:76% glycerol aqueous solution and propylene glycol mixture.

[0067] Experiment 3

[0068] A preparation method of a bionic blood with adjustable viscosity, comprising the following steps:

[0069] Step (1): Prepare a glycerol aqueous solution by mixing glycerol and pure water at a weight percentage of 28%:72%.

[0070] Step (2): Mix the glycerol aqueous solution and propylene glycol with weight percentages of 56.5%, 61%, 64.5%, 67.5%, and 68.8% respectively by weight percentage, slowly stir in a water bath at 40 - 70 °C for 2 min, and then let it stand for 15 min; its density and viscosity are as Figure 7 shown;

[0071] Step (3): Add sodium chloride with weights of 0.2%, 0.3%, 0.4%, 0.6%, and 0.8% respectively to the mixed solution obtained in step (2), slowly stir in a water bath at 40 - 70 °C for 5 min, and then let it stand for 1 h until the sodium chloride is gradually completely dissolved, with no bubbles and no floccules present, to obtain a colorless transparent liquid, which is the said bionic blood; its density and viscosity are as Figure 8 shown;

[0072] The component weight percentages are as shown in Table 3:

[0073]

[0074] Table III: Weight percentage of NaCl added to the 28%:72% glycerol aqueous solution and propylene glycol mixture.

[0075] The above experiments show that adding sodium chloride with a weight of about 0 - 0.9% to the above mixture increases the density of the mixture to the original density value. Adding propylene glycol to the glycerol aqueous solution increases the viscosity of the mixture and slightly reduces the density of the mixture; adding sodium chloride helps to increase the density of the mixture and has little effect on the viscosity.

Claims

1. A preparation method of a bionic blood with adjustable viscosity, which is used to prepare the bionic blood with adjustable viscosity, and is characterized in that: The bionic blood component includes glycerol, pure water, propylene glycol, and sodium chloride; The method includes the following steps: 1) Prepare an aqueous glycerol solution by mixing glycerol and pure water at a weight percentage of 24 - 28%: 72 - 76%; 2) Mix the aqueous glycerol solution obtained in step 1) and propylene glycol at a weight percentage of 31.2 - 100%: 0 - 68.8%, and the weight percentage of propylene glycol is not 0; 3) Add sodium chloride with a weight percentage of 0.1 - 0.9% to the mixture obtained in step 2). The viscosity of the resulting bionic blood is 1 - 15 mPa∙s, and the relative density remains 1.050 - 1.

060.

2. The preparation method of a bionic blood with adjustable viscosity according to claim 1, and is characterized in that: In step 1), the aqueous glycerol solution is prepared by mixing glycerol and pure water at a weight percentage of 26%: 74%.

3. The preparation method of a bionic blood with adjustable viscosity according to claim 1, and is characterized in that: In step 1), the aqueous glycerol solution is prepared by mixing glycerol and pure water at a weight percentage of 24%: 76%.

4. The preparation method of a bionic blood with adjustable viscosity according to claim 1, and is characterized in that: In step 1), the aqueous glycerol solution is prepared by mixing glycerol and pure water at a weight percentage of 28%: 72%.

5. The preparation method of a bionic blood with adjustable viscosity according to any one of claims 1-4, and is characterized in that: In step 2), the aqueous glycerol solution and propylene glycol are slowly stirred in a water bath at 40 - 70 °C for 2 min and then left to stand for 15 min.

6. The preparation method of a bionic blood with adjustable viscosity according to claim 5, and is characterized in that: In step 3), after adding sodium chloride to the mixture, it needs to be slowly stirred in a water bath at 40 - 70 °C for 5 min and then left to stand for 1 h until the sodium chloride is gradually completely dissolved, without bubbles or floccules, and a colorless transparent liquid is obtained, which is the bionic blood.

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