A polypeptide composition with repairing effect and its preparation method and application

Abstract

The present invention relates to the field of skin care products, and provides a polypeptide composition with a repairing effect, a preparation method thereof and an application thereof, wherein the composition comprises the following components by weight: 6-11 parts of a probiotic complex, 2-5 parts of white pondweed seed oil, 3-4 parts of hyaluronic acid, 5-8 parts of a composite Haematococcus pluvialis extract, 1-3 parts of trehalose, 7-10 parts of a scutellaria baicalensis extract, and 4-6 parts of a gentian root extract. The composition is synergistically compounded by probiotics, oligosaccharides and a variety of plant extracts, and the ingredients are safe and efficient, and have the effects of repairing the skin barrier and enhancing the skin resistance. The probiotic activity of the probiotic complex is stable, the skin beneficial flora is supplemented, and the self-flora is stimulated to secrete antimicrobial peptides, and together with the included composite antimicrobial peptides, it has the effect of repairing skin inflammation, and the composite Haematococcus pluvialis extract is added, which can better balance the skin microecology, further repair the skin barrier, and enhance the antioxidant function of the composition.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care products, and in particular to a polypeptide composition and application thereof in the preparation of skin care products. Background Art

[0002] The microbiome is closely related to the human body. It is distributed throughout the body, from inside to outside. The types and quantities of the microbiome need to reach a balance in order to maintain a stable physical condition. The skin is the largest protective organ of the body, especially on the face. The microbiome can give the skin a strong protective, repairing and resistant power, but once the microbiome loses balance, it will cause various skin problems, such as damaged skin barrier and water-oil imbalance, and then acne and sensitive skin will appear. With the in-depth research of scientists on the application of probiotics in skin care products, there are more and more examples of probiotics being used in skin care products today, but the survival of probiotics is a problem. The activity of probiotics has strict requirements on temperature and pH. Therefore, it is necessary to enhance the stability of probiotics in skin care products so that the efficacy of the product is not affected.

[0003] Currently, balancing the skin's microecology by adding exogenous bacteria to the skin is a novel skin care research. Usually, prebiotics and other substances are added to maintain the activity of probiotics, making the efficacy of probiotic skin care products more stable.

[0004] Patent No. 201410101906.2 discloses a Moringa multi-probiotic facial mask, which includes 60% yeast powder, 20% multi-probiotics, 10% Moringa extract, 5% natural honey, and 5% fresh milk in weight percentage. The probiotics are fermented by biological means under suitable conditions to increase the active ingredients in the plant extract. The mask has the effects of activating and repairing damaged skin cells and anti-oxidation. However, the probiotics play an auxiliary role and do not act directly on the face as an important skin care component. Summary of the invention

[0005] Purpose of the invention: The purpose of the present invention is to solve the deficiencies of the prior art and provide a polypeptide composition.

[0006] The technical solution of the present invention is as follows:

[0007] The present invention provides a polypeptide composition, comprising the following components by weight: a probiotic compound

[0008] 6-11 parts, white meadowfoam seed oil 2-5 parts, hyaluronic acid 3-4 parts, Haematococcus pluvialis extract 5-8 parts, trehalose 1-3 parts, Scutellaria baicalensis extract 7-10 parts, gentiana root extract 4-6 parts.

[0009] Preferably, the polypeptide composition comprises the following components in parts by weight: 9 parts of probiotic complex, 3 parts of meadowfoam seed oil, 3 parts of hyaluronic acid, 7 parts of composite Haematococcus pluvialis extract, 2 parts of trehalose, 9 parts of Scutellaria baicalensis extract, and 5 parts of Gentiana root extract.

[0010] For skin with an imbalanced microecology, probiotics can activate the bacteria to secrete antimicrobial peptides and balance the skin’s microecology. This can achieve a three-dimensional root treatment of balancing the skin’s microecology + protecting cell DNA + strengthening the skin barrier.

[0011] The rich astaxanthin contained in Haematococcus pluvialis has a good antioxidant effect. It can inhibit bacteria such as Staphylococcus aureus, provide nutrition to the skin and promote skin microcirculation.

[0012] Indonesian red algae strengthens the skin barrier and is a powerful prebiotic rich in antioxidants.

[0013] The present invention has found through a large number of experiments that the composition prepared by the present invention, which is compounded with a probiotic complex and a composite Haematococcus pluvialis extract, has the function of regulating the balance of skin microecology, and its barrier repair and antioxidant capabilities are significantly enhanced.

[0014] The preparation method of the probiotic complex of the present invention comprises the following steps:

[0015] Step 1: Heat and stir the white meadowfoam seed oil, dehydrated sorbitan fatty acid ester and distilled water to dissolve

[0016] The mixture is stirred at 60-75°C for 30-60 min, and then heated to 100-120°C for high temperature sterilization for 5-10 min to obtain a sterile oil and fat mixture;

[0017] Step 2: Cool the oil mixture to 25°C, add 60%-70% of the probiotic mixture by mass, continue to stir evenly, let it stand in a sterile environment for 2-3 hours, and then freeze-compress and dry it at a freezing temperature of -20--10°C.

[0018] The weight ratio of the meadowfoam seed oil, sorbitan fatty acid ester and distilled water is 10-14:4-6:12-16;

[0019] The probiotic mixture consists of probiotics, prebiotics and composite antimicrobial peptides, and the weight ratio of the probiotics, prebiotics and composite antimicrobial peptides is 6-8:4-6:1-2.

[0020] Preferably, the probiotics are bifidobacteria and enterococci in a mass ratio of 1:1.

[0021] Preferably, the prebiotics are stachyose and α-glucan oligosaccharides, and the weight ratio of stachyose to α-glucan oligosaccharides is 6-7:3-4.

[0022] Preferably, the composite antimicrobial peptide is composed of oligopeptide-1, oligopeptide-3, and oligopeptide-5.

[0023] The weight ratio of oligopeptide-3 to oligopeptide-5 is 1:1-3:1.

[0024] The preparation method of the Haematococcus pluvialis extract of the present invention comprises the following steps: cleaning the Haematococcus pluvialis and Indonesian red seaweed in a mass ratio of 11-14:1-2, freezing in liquid nitrogen for 5-10 minutes, and then grinding with a freezing mixing ball mill, adding the ground powder to an ethanol-acetone extract in a mass ratio of 6-12:3-5, stirring evenly, ultrasonically treating for 20-30 minutes, standing for 30-60 minutes, and then centrifuging, decompressing and compressing the obtained centrifuge, adding avocado oil in a mass ratio of 12-14:2-4 to the compressed liquid, and heating and refluxing for 1-2 hours to obtain a composite Haematococcus pluvialis extract;

[0025] The weight ratio of the powder to the ethanol-acetone extract is 12-18:26-35.

[0026] Preferably, the ultrasonic frequency is 25-35KHZ, and the centrifugal speed is 1500-2000rpm.

[0027] The invention provides a polypeptide composition and application thereof in skin care products. The skin care products are face cream, essence and toner.

[0028] Preferably, the composition is used in skin care products in an amount of 30%-50%.

[0029] Beneficial effects: The composition is a synergistic combination of probiotics, oligosaccharides and a variety of plant extracts. The ingredients are safe and efficient, and have the effects of repairing the skin barrier and enhancing skin resistance. The probiotic activity of the probiotic complex is stable, which supplements the beneficial bacteria of the skin and stimulates the self-bacteria to secrete antimicrobial peptides. Together with the contained composite antimicrobial peptides, it has the effect of repairing skin inflammation. Adding composite Haematococcus pluvialis extract can better balance the skin microecology, further repair the skin barrier, and enhance the antioxidant function of the composition. DETAILED DESCRIPTION

[0030] The present invention is described below with reference to specific embodiments. It is hereby stated that the embodiments selected herein are only used to illustrate and explain the present invention and are not used to limit the present invention.

[0031] Example 1

[0032] This embodiment provides a polypeptide composition, comprising the following components by weight: a probiotic compound

[0033] 11 parts of ingredients, 5 parts of stachyose, 4 parts of hyaluronic acid, 8 parts of composite Haematococcus pluvialis extract, 3 parts of trehalose, 10 parts of Scutellaria baicalensis extract, and 6 parts of Gentiana root extract.

[0034] The preparation method of the probiotic complex described in this embodiment comprises the following steps:

[0035] Step 1: Heat and stir the white meadowfoam seed oil, dehydrated sorbitan fatty acid ester and distilled water to dissolve

[0036] The mixture was stirred at 75°C for 60 min and then heated to 120°C for high temperature sterilization for 10 min to obtain a sterile oil and fat mixture;

[0037] Step 2: Cool the oil mixture to 25°C, add 70% of the probiotic mixture by mass, continue to stir evenly, let it stand in a sterile environment for 3 hours, and then freeze-compress and dry it at a freezing temperature of -10°C.

[0038] The weight ratio of the meadowfoam seed oil, sorbitan fatty acid ester and distilled water is 14:6:16;

[0039] The probiotic mixture consists of probiotics, prebiotics and composite antimicrobial peptides, and the weight ratio of the probiotics, prebiotics and composite antimicrobial peptides is 8:6:2.

[0040] The probiotics are bifidobacteria and enterococci in a mass ratio of 1:1.

[0041] The prebiotics are stachyose and a-glucan oligosaccharide, and the weight ratio of stachyose to a-glucan oligosaccharide is 7:4.

[0042] The composite antimicrobial peptide is composed of oligopeptide-1, oligopeptide-3, and oligopeptide-5.

[0043] The weight ratio of oligopeptide-5 is 1:2:1.

[0044] The preparation method of the composite Haematococcus pluvialis extract described in this embodiment is as follows: clean the Haematococcus pluvialis and the Indonesian red seaweed in a mass ratio of 14:2, freeze them in liquid nitrogen for 10 minutes, and then grind them with a freezing mixing ball mill, add the ground powder into an ethanol-acetone extract in a mass ratio of 12:5, stir evenly, ultrasonically treat for 30 minutes, let it stand for 60 minutes, and then centrifuge, decompress and compress the obtained centrifuge, add avocado oil in a mass ratio of 14:4 to the compressed liquid, and heat and reflux for 2 hours to obtain the composite Haematococcus pluvialis extract;

[0045] The weight ratio of the powder to the ethanol-acetone extract is 18:35.

[0046] The ultrasonic frequency is 35KHZ and the centrifugal speed is 2000rpm.

[0047] Example 2

[0048] The present embodiment provides a polypeptide composition, comprising the following components in parts by weight: 6 parts of a probiotic complex, 2 parts of meadowfoam seed oil, 3 parts of hyaluronic acid, 5 parts of a composite Haematococcus pluvialis extract, 1 part of trehalose, 7 parts of a scutellaria baicalensis extract, and 4 parts of a gentian root extract.

[0049] The preparation method of the probiotic complex described in this embodiment comprises the following steps:

[0050] Step 1: Heat and stir the white meadowfoam seed oil, dehydrated sorbitan fatty acid ester and distilled water to dissolve

[0051] The mixture was stirred at 60°C for 30 min and then heated to 100°C for high temperature sterilization for 5 min to obtain a sterile oil and fat mixture;

[0052] Step 2: Cool the oil mixture to 25°C, add 60% of the probiotic mixture by mass, continue to stir evenly, let it stand in a sterile environment for 2 hours, and then freeze-compress and dry it at a freezing temperature of -20°C.

[0053] The weight ratio of the meadowfoam seed oil, sorbitan fatty acid ester and distilled water is 10:4:12;

[0054] The probiotic mixture consists of probiotics, prebiotics and composite antimicrobial peptides, and the weight ratio of the probiotics, prebiotics and composite antimicrobial peptides is 6:4:1.

[0055] The probiotics are bifidobacteria and enterococci in a mass ratio of 1:1.

[0056] The prebiotics are stachyose and a-glucan oligosaccharide, and the weight ratio of stachyose to a-glucan oligosaccharide is 6:3.

[0057] The composite antimicrobial peptide is composed of oligopeptide-1, oligopeptide-3, and oligopeptide-5.

[0058] The weight ratio of oligopeptide-5 is 1:1:1.

[0059] The preparation method of the composite Haematococcus pluvialis extract described in this embodiment is as follows: clean the Haematococcus pluvialis and the Indonesian red seaweed in a mass ratio of 11:1, freeze them in liquid nitrogen for 5 minutes, and then grind them with a freezing mixing ball mill, add the ground powder into an ethanol-acetone extract in a mass ratio of 6:3, stir evenly, ultrasonically treat for 20 minutes, let it stand for 30 minutes, and then centrifuge, decompress and compress the obtained centrifuge, add avocado oil in a mass ratio of 12:2 to the compressed liquid, and heat and reflux for 1 hour to obtain the composite Haematococcus pluvialis extract;

[0060] The weight ratio of the powder to the ethanol-acetone extract is 12:26.

[0061] The ultrasonic frequency is 25KHZ and the centrifugal speed is 1500rpm.

[0062] Example 3

[0063] The present embodiment provides a polypeptide composition, comprising the following components in parts by weight: 9 parts of a probiotic complex, 3 parts of meadowfoam seed oil, 3 parts of hyaluronic acid, 7 parts of a composite Haematococcus pluvialis extract, 2 parts of trehalose, 9 parts of a scutellaria baicalensis extract, and 5 parts of a gentian root extract.

[0064] The preparation method of the probiotic complex described in this embodiment comprises the following steps:

[0065] Step 1: Heat and stir the white meadowfoam seed oil, dehydrated sorbitan fatty acid ester and distilled water to dissolve

[0066] The mixture was stirred at 68°C for 45 min and then heated to 110°C for 7 min to obtain a sterile oil and fat mixture.

[0067] Step 2: Cool the oil mixture to 25°C, add 65% of the probiotic mixture by mass, continue to stir evenly, let it stand in a sterile environment for 2 hours, and then freeze-compress and dry it at a freezing temperature of -15°C.

[0068] The weight ratio of the meadowfoam seed oil, sorbitan fatty acid ester and distilled water is 12:5:14;

[0069] The probiotic mixture is composed of probiotics, prebiotics and composite antimicrobial peptides, and the weight ratio of probiotics, prebiotics and composite antimicrobial peptides is 7:5:1;

[0070] The probiotics are bifidobacteria and enterococci in a mass ratio of 1:1;

[0071] The prebiotics are stachyose and a-glucan oligosaccharide, and the weight ratio of stachyose to a-glucan oligosaccharide is 6:4;

[0072] The composite antimicrobial peptide is composed of oligopeptide-1, oligopeptide-3 and oligopeptide-5.

[0073] The weight ratio of oligopeptide-3 to oligopeptide-5 is 1:3:1.

[0074] The preparation method of the composite Haematococcus pluvialis extract described in this embodiment is as follows: clean the Haematococcus pluvialis and the Indonesian red seaweed in a mass ratio of 12:1.5, freeze them in liquid nitrogen for 7 minutes, and then grind them with a freezing mixing ball mill, add the ground powder to an ethanol-acetone extract in a mass ratio of 9:4, stir evenly, ultrasonically treat for 25 minutes, let stand for 45 minutes, and then centrifuge, decompress and compress the obtained centrifuge, add avocado oil in a mass ratio of 13:3 to the compressed liquid, and heat and reflux for 1.5 hours to obtain the composite Haematococcus pluvialis extract;

[0075] The weight ratio of the powder to the ethanol-acetone extract is 15:30.

[0076] The weight ratio of the powder to the ethanol-acetone extract is 15:31.

[0077] The ultrasonic frequency is 30KHZ and the centrifugal speed is 1700rpm.

[0078] Comparative Example 1

[0079] The difference between Comparative Example 1 and Example 3 is that the composite Haematococcus pluvialis extract is not added.

[0080] Comparative Example 2

[0081] The difference between Comparative Example 2 and Example 3 is that no probiotic complex is added.

[0082] Comparative Example 3

[0083] The difference between Comparative Example 3 and Example 3 is that probiotics are added to the composition instead of the probiotic complex. The probiotics are bifidobacteria and enterococci in a mass ratio of 1:1, and the added amount is the same as that of Example 3.

[0084] Comparative Example 4

[0085] The difference between Comparative Example 4 and Example 3 is that the probiotic composition does not contain any composite antimicrobial peptide.

[0086] Comparative Example 4

[0087] The difference between Comparative Example 4 and Example 3 is that the preparation method of the probiotic complex is different, and no prebiotics are added during the preparation of the probiotic complex.

[0088] Comparative Example 5

[0089] The difference between Comparative Example 5 and Example 3 is that the preparation method of the composite Haematococcus pluvialis extract is different, and Indonesian red seaweed is not added during the preparation of the composite Haematococcus pluvialis extract.

[0090] The compositions prepared in Examples 1-3 and Comparative Examples 1-5 were added to common moisturizing facial cream purchased on the market in an amount of 30%, and stirred evenly to obtain a probiotic facial cream.

[0091] Experiment 1: Microecological barrier restoration test

[0092] Before the test, 36 mice with skin inflammation were prepared and divided into 9 groups. After wiping the inflammation site with physiological saline, they were wiped with sterile cotton swabs soaked in FBS buffer, the head of the cotton swab was removed and placed in a 1ml sterile FBS test tube, and the FBS solution was inoculated into the culture medium to detect the main colonies and their numbers. Within 10 days thereafter, 0.1ml of the samples of Examples 1-3 and Comparative Examples 1-5 were taken every day to wipe the inflammation sites of 8 groups of mice, and the remaining group was wiped with physiological saline as a blank control. On the 5th day and the 10th day, the flora and its number were detected at this time, and the average of the flora number detected before and after each group of mice was calculated, and the average growth rate or average reduction rate of each group of flora was obtained. The test results are shown in Table 1.

[0093] Table 1. Changes in average growth rate of bacterial population

[0094]

[0095] As shown in Table 1, the damaged microecology of the inflammatory part of the mice was well suppressed after applying the samples provided in the present embodiments 1-3, and the beneficial bacteria carried grew well on the skin. On the 5th day, the colony growth rate was within the range of 70%-90%. From this comparative example 1-2, it can be seen that the probiotic activity in the probiotic complex prepared by the present invention is high, and the compound Haematococcus pluvialis extract is added to the composition, which can better inhibit the growth of harmful bacteria and balance the skin microecology; as shown in comparative examples 3-5, the preparation method of the present invention prepares the probiotic complex, adds the composite antimicrobial peptide, the prebiotic and the probiotic compound, can restore the balance of the flora, and the compound Haematococcus pluvialis extract is more effective, and the effect of balancing the flora is more significant.

[0096] Experiment 2: Skin barrier repair test

[0097] 270 hairless male squirrels of the same age were prepared and divided into 9 groups, each with 30 mice. The moisture content and skin elasticity of the back skin of each group of squirrels were tested with a skin elasticity tester and a skin moisture tester, and the data were recorded. After wiping the back skin with 78% ethanol for 5 minutes, the skin was exposed to UVB rays for 20 minutes, so that the back skin barrier of the hairless squirrels was slightly damaged. 0.1 ml of the products of Examples 1-3 and Comparative Examples 1-5 were applied to the back skin of each group of hairless squirrels, and the remaining group was not treated. After 1 hour and 3 hours, the moisture content and skin elasticity of the back skin of each group of hairless squirrels were tested again, and the data were recorded to know the skin barrier repair of each group of hairless squirrels. The test results are shown in Table 2.

[0098] Table 2.

[0099]

[0100] As can be seen from Table 2, Examples 1-3 all have a fast and significant repair effect on the damaged skin barrier; Comparative Examples 1-2 show that the probiotic complex prepared by the present invention and the composite Haematococcus pluvialis extract are compounded, and the repair effect on the skin barrier is more significant than the single addition of the probiotic complex or the composite Haematococcus pluvialis extract; Comparative Examples 3-5 show that the preparation method adopted by the present invention is better than the probiotic complex and the composite Haematococcus pluvialis extract prepared by other methods, and the antimicrobial peptides produced by the probiotics work synergistically with the composite antimicrobial peptides, and are more effective than the probiotics and Haematococcus pluvialis directly added to the composition, and can repair and strengthen the skin barrier from multiple aspects such as colony regulation and cell energy supply.

[0101] Experiment 3: Antioxidant function of the skin

[0102] DPPH is a stable organic free radical that exists stably in organic solvents. It acts on free radicals during antioxidant reactions or indirectly consumes substances that are prone to generating free radicals.

[0103] Weigh 2 mg of DPPH reagent, dissolve it in anhydrous ethanol, and use a 10 ml volumetric flask to make up the volume. The solution is purple. Prepare 10 groups of solutions, pour them into brown reagent bottles, add the samples of Examples 1-3 and Comparative Examples 1-5 to 8 groups, and use vitamin C as a positive control in the other two groups. Use anhydrous ethanol as a blank solution, stir evenly, and let stand for 20 minutes, then measure the optical density (OD). Two parallel experiments were performed in this experiment, and the average optical density value was taken to calculate the DPPH scavenging activity (DSA). The results are shown in Table 3.

[0104] DPPH scavenging activity (%) = [1-(sample OD-blank OD) / control OD] × 100

[0105] Table 3.

[0106]

[0107] It can be seen from Table 3 that Examples 1-3 are probiotic compositions prepared within the scope of the present invention, which have good antioxidant effects, reaching more than 90%, which are significantly higher than the positive control group; it can be seen from Comparative Examples 1-5 that the composite Haematococcus pluvialis extract is a composite extraction of Indonesian red algae and Haematococcus pluvialis, has rich and high-purity antioxidant components, and is compounded with the probiotic complex to significantly enhance the antioxidant effect.

[0108] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A polypeptide composition, characterized in that: The invention comprises the following components in parts by weight: 6-11 parts of probiotic complex, 2-5 parts of meadowfoam seed oil, 3-4 parts of hyaluronic acid, 5-8 parts of composite Haematococcus pluvialis extract, 1-3 parts of trehalose, 7-10 parts of scutellaria baicalensis extract, and 4-6 parts of gentian root extract; The preparation method of the probiotic complex is as follows: step 1: heating and stirring white meadowfoam seed oil, dehydrated sorbitan fatty acid ester and distilled water to dissolve, the stirring temperature is 60-75° C., the stirring time is 30-60 min, heating to 100-120° C. and performing high temperature sterilization for 5-10 min to obtain a sterile oil and fat mixture; Step 2: Cool the oil mixture to 25°C, add 60%-70% of the probiotic mixture by mass, continue to stir evenly, let it stand in a sterile environment for 2-3 hours, and then freeze-compress and dry it at a freezing temperature of -20--10°C; The weight ratio of the meadowfoam seed oil, sorbitan fatty acid ester and distilled water is 10-14:4-6:12-16; The probiotic mixture consists of probiotics, prebiotics and composite antimicrobial peptides, and the weight ratio of probiotics, prebiotics and composite antimicrobial peptides is 6-8:4-6:1-2; The probiotics are bifidobacteria and enterococci in a mass ratio of 1:1; The prebiotics are stachyose and a-glucan oligosaccharide, and the weight ratio of stachyose to a-glucan oligosaccharide is 6-7:3-4; The composite antimicrobial peptide is composed of oligopeptide-1, oligopeptide-3 and oligopeptide-5, and the weight ratio of oligopeptide-1, oligopeptide-3 and oligopeptide-5 is 1:1-3:1; The preparation method of the composite Haematococcus pluvialis extract is as follows: clean the Haematococcus pluvialis and Indonesian red seaweed in a mass ratio of 11-14:1-2, freeze them in liquid nitrogen for 5-10 minutes, and then grind them with a freezing mixing ball mill, add the ground powder into an ethanol-acetone extract in a mass ratio of 6-12:3-5, stir evenly, ultrasonically treat for 20-30 minutes, stand for 30-60 minutes, and then centrifuge, decompress and compress the obtained centrifuge liquid, add avocado oil in a mass ratio of 12-14:2-4 to the compressed liquid, and heat and reflux for 1-2 hours to obtain the composite Haematococcus pluvialis extract; The weight ratio of the powder to the ethanol-acetone extract is 12-18:26-35.

2. A polypeptide composition according to claim 1, characterized in that: The invention comprises the following components in parts by weight: 9 parts of probiotic complex, 3 parts of meadowfoam seed oil, 3 parts of hyaluronic acid, 7 parts of composite Haematococcus pluvialis extract, 2 parts of trehalose, 9 parts of scutellaria baicalensis extract and 5 parts of gentian root extract.

3. A polypeptide composition according to claim 1, characterized in that: The ultrasonic frequency is 25-35KHZ, and the centrifugal speed is 1500-2000rpm.

4. Use of a polypeptide composition according to claim 1 in preparing a skin care product, characterized in that: The skin care products are face cream, essence and toner.

5. Use of a polypeptide composition according to claim 4 in preparing skin care products, characterized in that: The application amount of the composition in skin care products is 30%-50%.

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