A cutting method for Cinnamomum camphora (L.) Presl. var. borneoliferum H. W. Li

Abstract

The present application relates to a cutting method for Cinnamomum camphora var. borneolifera, belonging to the technical field of Cinnamomum camphora var. borneolifera planting. The cutting method comprises the following steps: obtaining cuttings of Cinnamomum camphora var. borneolifera; inserting the cuttings of Cinnamomum camphora var. borneolifera into a cutting substrate, and then performing post-cutting management to obtain finished seedlings of Cinnamomum camphora var. borneolifera; wherein, by weight parts, the cutting substrate comprises the following components: 40-60 parts of red soil, 20-30 parts of perlite, 15-20 parts of sophorolipid and 100 parts of water. By using red soil, perlite and sophorolipid with appropriate ratios as the cutting substrate for Cinnamomum camphora var. borneolifera, the present invention can quickly promote the rooting of cuttings of Cinnamomum camphora var. borneolifera and strengthen the roots of cutting seedlings, thereby improving the survival rate of cutting seedlings of Cinnamomum camphora var. borneolifera and effectively solving the problems of low survival rate of cutting seedlings and long rooting time existing in the existing cutting method for Cinnamomum camphora var. borneolifera.

Description

Technical Field

[0001] This application relates to the technical field of Cinnamomum camphora (L.) Presl. var. borneolifera (Y. C. Yang & P. H. Huang) H. W. Li cultivation, and particularly relates to a cutting method for Cinnamomum camphora (L.) Presl. var. borneolifera (Y. C. Yang & P. H. Huang) H. W. Li. Background Art

[0002] Cinnamomum camphora (L.) Presl. var. borneolifera (Y. C. Yang & P. H. Huang) H. W. Li belongs to typical tree species in tropical rainforests. It is a specific chemical type among the five chemical types of Cinnamomum camphora (L.) Presl. whose fresh leaf essential oil is rich in d-borneol (natural borneol). It is the best plant resource for producing the precious and rare medicinal material natural borneol at present, and has huge market demand and potential.

[0003] The propagation methods of Lauraceae plants mainly include sowing propagation, grafting propagation and cutting propagation. Among them, cutting propagation is a propagation method that uses small branches of Cinnamomum camphora (L.) Presl. var. borneolifera (Y. C. Yang & P. H. Huang) H. W. Li with mature leaves (mother leaves) and strong leaf buds for cutting to form independent plants. The plants cultivated by this method grow faster than sowing seedlings and can be cultivated into large seedlings in a short time. However, there are problems of low survival rate of cuttings and long rooting time in the existing cutting methods of Cinnamomum camphora (L.) Presl. var. borneolifera (Y. C. Yang & P. H. Huang) H. W. Li, which greatly limits its application. Summary of the Invention

[0004] This application provides a cutting method for Cinnamomum camphora (L.) Presl. var. borneolifera (Y. C. Yang & P. H. Huang) H. W. Li to solve the problems of low survival rate of cuttings and long rooting time in the existing cutting methods of Cinnamomum camphora (L.) Presl. var. borneolifera (Y. C. Yang & P. H. Huang) H. W. Li.

[0005] In the first aspect, this application provides a cutting method for Cinnamomum camphora (L.) Presl. var. borneolifera (Y. C. Yang & P. H. Huang) H. W. Li, and the cutting method includes the following steps:

[0006] Obtain cuttings of Cinnamomum camphora (L.) Presl. var. borneolifera (Y. C. Yang & P. H. Huang) H. W. Li;

[0007] Insert the cuttings of Cinnamomum camphora (L.) Presl. var. borneolifera (Y. C. Yang & P. H. Huang) H. W. Li into a cutting substrate, and then carry out post-cutting management to obtain finished seedlings of Cinnamomum camphora (L.) Presl. var. borneolifera (Y. C. Yang & P. H. Huang) H. W. Li;

[0008] Among them, by weight, the cutting substrate includes the following components: 40-60 parts of red soil, 20-30 parts of perlite, 15-20 parts of sophorolipid and 100 parts of water.

[0009] Further, the sum of the weight parts of the red soil and the perlite is X, the weight part of the sophorolipid is Y, and X and Y satisfy relationship one;

[0010] Relationship one: 0.20 ≤ Y / X ≤ 0.30.

[0011] Further, by weight, the cutting substrate includes the following components:

[0012] 42 parts of red soil, 28 parts of perlite, 18 parts of sophorolipid and 100 parts of water.

[0013] Further, the sophorolipid is acid-type sophorolipid.

[0014] Further, the steps of obtaining the Cinnamomum camphora cuttings include the following process:

[0015] Select ear shoots with an ear length of 2 - 5 cm, an ear diameter of 0.2 - 0.6 cm, at least one leaf, and at least two nodes for shearing to obtain short ears;

[0016] Soak the short ears in a disinfectant for the first soaking, and then transfer them to a rooting agent for the second soaking to obtain the Cinnamomum camphora cuttings.

[0017] Further, the disinfectant is composed of carbendazim and water with a weight ratio of 1:(900 - 1100); the time for the first soaking is 5 - 10 minutes.

[0018] Further, the rooting agent is composed of ABT rooting powder, naphthaleneacetic acid, and water with a weight ratio of 1:1:(9000 - 11000); the time for the second soaking is 1 - 2 hours.

[0019] Further, the cutting depth of the Cinnamomum camphora cuttings is 4 - 5 cm, and the cutting spacing is 4 - 6 cm.

[0020] Further, the steps of the management after cutting include: controlling the ground temperature between 29 - 32 °C, the greenhouse temperature between 25 - 28 °C, the humidity in the greenhouse between 70% - 90%, and the shading rate in the greenhouse between 80% - 90%.

[0021] The above technical solutions provided by the embodiments of the present application have at least the following advantages compared with the prior art:

[0022] The embodiments of the present application provide a cutting method for Cinnamomum camphora. The inventors found that by using red soil, perlite, and sophorolipid with appropriate ratios as the cutting substrate for Cinnamomum camphora, it can quickly promote the rooting of Cinnamomum camphora cuttings and strengthen the roots of cutting seedlings, thereby improving the survival rate of Cinnamomum camphora cutting seedlings and effectively solving the problems of low survival rate of cutting seedlings and long rooting time existing in the existing cutting methods for Cinnamomum camphora. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The accompanying drawings herein are incorporated into the specification and constitute a part of this specification, showing embodiments consistent with the present application, and are used together with the specification to explain the principles of the present application.

[0024] To more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the accompanying drawings required for use in the description of the embodiments or the prior art. Obviously, for those of ordinary skill in the art, other accompanying drawings can also be obtained based on these drawings without creative efforts.

[0025] Figure 1A schematic flow chart of a borneol camphor cutting method provided in an embodiment of the present application. DETAILED DESCRIPTION

[0026] In order to make the purpose, technical solution and advantages of the embodiments of the present application clearer, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of this application.

[0027] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in this application can be purchased from the market or prepared by existing methods.

[0028] In the first aspect, the present application provides a method for cutting of borneol camphor, such as Figure 1 As shown, the cutting method comprises the following steps:

[0029] Get borneol camphor cuttings;

[0030] Inserting the borneol camphor cuttings into a cutting medium, and then performing post-cutting management to obtain borneol camphor finished seedlings;

[0031] Wherein, the cutting medium includes the following components in parts by weight: 40-60 parts of red soil, 20-30 parts of perlite, 15-20 parts of sophorolipids and 100 parts of water.

[0032] The embodiment of the present application provides a method for propagating borneol camphor. The inventors have found that by using red soil, perlite and sophorolipids in an appropriately proportioned manner as the propagation medium for borneol camphor, the rooting of borneol camphor cuttings and the strengthening of the root system of the propagation seedlings can be quickly promoted, thereby improving the survival rate of the propagation seedlings for borneol camphor, and effectively solving the problems of low survival rate of the propagation seedlings and long rooting time in the existing propagation methods for borneol camphor.

[0033] The red soil described in this application refers to red soil, which is mainly distributed in the low mountain and hilly areas south of the Yangtze River in China. In some specific embodiments, the weight percentage of the red soil can be 40 parts, 41 parts, 42 parts, 43 parts, 44 parts, 45 parts, 46 parts, 47 parts, 48 ​​parts, 49 parts, 50 parts, 51 parts, 52 parts, 53 parts, 54 parts, 55 parts, 56 parts, 57 parts, 58 parts, 59 parts, 60 parts, etc.

[0034] The perlite described in the present application is a kind of acidic lava from volcanic eruptions, which is a glassy rock formed by rapid cooling and has good air permeability and drainage. In some specific embodiments, the weight proportion of the perlite can be 20 parts, 21 parts, 22 parts, 23 parts, 24 parts, 25 parts, 26 parts, 27 parts, 28 parts, 29 parts, 30 parts, etc.

[0035] The sophorolipids described in this application are microbial secondary metabolites produced by Candida using sugar and vegetable oil as carbon sources and fermentation processes under certain conditions, mainly including lactone type and acid type, which can be purchased directly from Shaanxi Deguan Biotechnology Co., Ltd.; used in combination with red soil and perlite, it not only provides a slightly acidic growth environment for borneol camphor cuttings, but also can quickly promote the rooting of borneol camphor cuttings and strengthen the root system of cuttings. In some specific embodiments, the weight proportions of the sophorolipids can be 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts, etc.

[0036] In some specific embodiments, the method for preparing the cutting medium comprises: stirring and mixing red soil, perlite, sophorolipids and water evenly; preferably, first stirring and mixing sophorolipids and water evenly, and then adding red soil and perlite to mix.

[0037] In some specific embodiments, the cutting depth of the Camphor Cinnamomum camphora cuttings is 4 to 5 cm, and the cutting spacing is 4 to 6 cm.

[0038] As an implementation method of the present application, the sum of the weight percentages of the red soil and the perlite is X, the weight percentage of the sophorolipid is Y, and X and Y satisfy the relationship 1;

[0039] The relationship formula 1: 0.20≤Y / X≤0.30.

[0040] The applicant has found that by adding an appropriate amount of sophorolipids, it can quickly promote the rooting of borneol camphor cuttings and strengthen the root system of cuttings. However, when the dosage of sophorolipids is too much, the survival rate of borneol camphor cuttings will be reduced to a certain extent, which may be related to the acidity of the system; at the same time, when the dosage of sophorolipids is too little, the enhancement effect is not obvious.

[0041] It should be noted that "Y / X" in the relationship formula 1 is expressed as "the quotient of Y divided by X", which specifically refers to the ratio of "the weight fraction of the sophorolipid is Y" and "the sum of the weight fractions of the red soil and the perlite is X".

[0042] As an implementation of the present application, the cutting matrix includes the following components in parts by weight:

[0043] 42 parts of red soil, 28 parts of perlite, 18 parts of sophorolipids and 100 parts of water.

[0044] When the cutting substrate of the present application is controlled to the above ratio, the survival rate of the cuttings of Cinnamomum camphora var. borneolifera is high.

[0045] As an implementation manner of the embodiment of the present application, the sophorolipid is an acid-type sophorolipid.

[0046] Preferably, the acid-type sophorolipid is selected in the present application, and the enhancing promotion effect is more obvious.

[0047] As an implementation manner of the embodiment of the present application, the steps of obtaining the cuttings of Cinnamomum camphora var. borneolifera include the following process:

[0048] Select cuttings with a spike length of 2 - 5 cm, a spike diameter of 0.2 - 0.6 cm, at least 1 leaf and at least 2 nodes for shearing to obtain short spikes;

[0049] Place the short spikes in a disinfectant for the first soaking, and then transfer them to a rooting agent for the second soaking to obtain the cuttings of Cinnamomum camphora var. borneolifera.

[0050] The present application selects short spikes with appropriate spike length and spike diameter, which is beneficial to improving the survival rate of the cutting method of Cinnamomum camphora var. borneolifera.

[0051] In some specific embodiments, the disinfectant is composed of carbendazim and water with a weight ratio of 1:(900 - 1100); the time of the first soaking is 5 - 10 minutes.

[0052] In some specific embodiments, the rooting agent is composed of ABT rooting powder, naphthaleneacetic acid and water with a weight ratio of 1:1:(9000 - 11000); the time of the second soaking is 1 - 2 hours.

[0053] As an implementation manner of the embodiment of the present application, the steps of the management after cutting include: controlling the ground temperature between 29 - 32 °C, the greenhouse temperature between 25 - 28 °C, the humidity in the greenhouse between 70% - 90%, and the shading rate in the greenhouse between 80% - 90%.

[0054] When the cuttings of Cinnamomum camphora var. borneolifera after cutting are controlled to the above parameters in the present application, the cultivation conditions are suitable and beneficial to seedling formation.

[0055] It should be noted that for the raw materials of each component in the cutting method of Cinnamomum camphora var. borneolifera provided above in the present application, if there is no special limitation or description, commercially available products can be directly used; at the same time, for the operation steps in the preparation method of the cutting method of Cinnamomum camphora var. borneolifera provided in the present application, if there is no special limitation or description, the conventional methods in the art can be used, and will not be elaborated here one by one.

[0056] The present application will be further described below in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present application and not to limit the scope of the present application. For the experimental methods without specific conditions noted in the following embodiments, they are generally determined according to national standards. If there is no corresponding national standard, they are carried out according to general international standards, conventional conditions, or conditions recommended by the manufacturer.

[0057] Example 1

[0058] This example provides a cutting method for Cinnamomum camphora var. borneolifera, and the cutting method for Cinnamomum camphora var. borneolifera includes the following process:

[0059] Select ear shoots with an ear length of 3 - 4 cm, an ear diameter of 0.3 - 0.5 cm, at least 1 leaf, and at least 2 nodes for shearing to obtain short ears;

[0060] Immerse the short ears in a disinfectant for the first soaking, and then transfer them to a rooting agent for the second soaking to obtain the cuttings of Cinnamomum camphora var. borneolifera; wherein, the disinfectant is composed of carbendazim and water with a weight ratio of 1:1000; the time for the first soaking is 8 minutes; the rooting agent is composed of ABT-1 rooting powder, naphthaleneacetic acid, and water with a weight ratio of 1:1:10000; the time for the second soaking is 1.5 hours;

[0061] Insert the cuttings of Cinnamomum camphora var. borneolifera into the cutting substrate, with a cutting depth of 4 - 5 cm, a cutting plant spacing of 4 - 6 cm, a total of 200 plants, and then carry out post-cutting management. The ground temperature is controlled between 29 - 32 °C, the greenhouse temperature is controlled between 25 - 28 °C, the humidity in the greenhouse is controlled at about 90%, and the shading rate in the greenhouse is about 85% to obtain the finished seedlings of Cinnamomum camphora var. borneolifera; wherein, by weight parts (unit: g), the cutting substrate includes the following components: 42 parts of red soil, 28 parts of perlite, 18 parts of sophorolipid, and 100 parts of water. The sum of the weight parts of the red soil and the perlite is X, the weight part of the sophorolipid is Y, and "Y / X" is 0.26; the red soil is the red soil at a depth of 35 - 60 cm from the ground surface, with a sand content of 30 wt% - 50 wt%; the sophorolipid is acid-type sophorolipid, purchased from Shaanxi Deguan Biotechnology Co., Ltd.

[0062] Example 2

[0063] This example provides a cutting method for Cinnamomum camphora var. borneolifera, and the difference from Example 1 is only that: by weight parts, the cutting substrate includes the following components: 40 parts of red soil, 20 parts of perlite, 15 parts of sophorolipid, and 100 parts of water, and "Y / X" is 0.25; the remaining steps and parameters are the same.

[0064] Example 3

[0065] This example provides a cutting method for Cinnamomum camphora var. borneolifera, which is only different from Example 1 in that: by weight, the cutting substrate comprises the following components: 60 parts of red soil, 30 parts of perlite, 20 parts of sophorolipid and 100 parts of water, and "Y / X" is 0.22; the remaining steps and parameters are the same.

[0066] Example 4

[0067] This example provides a cutting method for Cinnamomum camphora var. borneolifera, which is only different from Example 1 in that: by weight, the cutting substrate comprises the following components: 60 parts of red soil, 30 parts of perlite, 15 parts of sophorolipid and 100 parts of water, and "Y / X" is 0.17; the remaining steps and parameters are the same.

[0068] Example 5

[0069] This example provides a cutting method for Cinnamomum camphora var. borneolifera, which is only different from Example 1 in that: by weight, the cutting substrate comprises the following components: 40 parts of red soil, 20 parts of perlite, 20 parts of sophorolipid and 100 parts of water, and "Y / X" is 0.33; the remaining steps and parameters are the same.

[0070] Example 6

[0071] This example provides a cutting method for Cinnamomum camphora var. borneolifera, which is only different from Example 1 in that: the sophorolipid is lactone-type sophorolipid.

[0072] Comparative Example 1

[0073] This example provides a cutting method for Cinnamomum camphora var. borneolifera, which is only different from Example 1 in that: the dosage of the sophorolipid is adjusted to 30 parts (i.e., the dosage of the sophorolipid is excessive); the remaining steps and parameters are the same.

[0074] Comparative Example 2

[0075] This example provides a cutting method for Cinnamomum camphora var. borneolifera, which is only different from Example 1 in that: the dosage of the sophorolipid is adjusted to 5 parts (i.e., the dosage of the sophorolipid is too little); the remaining steps and parameters are the same.

[0076] Comparative Example 3

[0077] This example provides a cutting method for Cinnamomum camphora var. borneolifera, which is only different from Example 1 in that: the sophorolipid is adjusted to rhamnolipid; the remaining steps and parameters are the same.

[0078] Comparative Example 4

[0079] This example provides a cutting method for Cinnamomum camphora var. borneolifera, which is only different from Example 1 in that: the sophorolipid is adjusted to trehalolipid; the remaining steps and parameters are the same.

[0080] Comparative Example 5

[0081] This example provides a cutting method for Cinnamomum camphora var. borneolifera, which is only different from Example 1 in that: the dosage of perlite is adjusted to 0 part, and the dosage of red soil is adjusted to 70 parts (that is, no perlite is added, and all are based on red soil); the remaining steps and parameters are the same.

[0082] Comparative Example 6

[0083] This example provides a cutting method for Cinnamomum camphora var. borneolifera, which is only different from Example 1 in that: the dosage of perlite is adjusted to 70 parts, and the dosage of red soil is adjusted to 0 part (that is, no red soil is added, and all are based on perlite); the remaining steps and parameters are the same.

[0084] In the cutting methods for Cinnamomum camphora var. borneolifera provided in the above Examples 1 to 6 and Comparative Examples 1 to 6, the survival rate of the cuttings and the rooting time were statistically analyzed, and the results are shown in Table 1.

[0085] Table 1

[0086]

[0087]

[0088] In summary, the embodiments of the present application provide a cutting method for Cinnamomum camphora var. borneolifera. By using red soil, perlite, and sophorolipid with appropriate ratios as the cutting substrate for Cinnamomum camphora var. borneolifera, it can quickly promote the rooting of the cuttings of Cinnamomum camphora var. borneolifera and strengthen the roots of the cuttings, thereby improving the survival rate of the cuttings of Cinnamomum camphora var. borneolifera and effectively solving the problems of low survival rate of cuttings and long rooting time existing in the existing cutting methods for Cinnamomum camphora var. borneolifera.

[0089] The various embodiments of the present application may exist in the form of a range; it should be understood that the description in the form of a range is only for convenience and brevity, and should not be construed as a rigid limitation on the scope of the present application; therefore, it should be considered that the description of the range has specifically disclosed all possible sub-ranges and single values within that range. For example, it should be considered that the description of the range from 1 to 6 has specifically disclosed sub-ranges such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., and single numbers within that range, such as 1, 2, 3, 4, 5, and 6, regardless of what the range is. Additionally, whenever a numerical range is indicated herein, it means including any cited number (fraction or integer) within the indicated range.

[0090] In this application, unless otherwise stated, the orientation terms such as "upper" and "lower" specifically refer to the drawing directions in the attached drawings. Additionally, in the description of the specification of this application, the terms "including", "comprising", etc. mean "including but not limited to". In this text, relational terms such as "first" and "second" are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. In this text, "and / or" describes the associated relationship of associated objects and indicates that three relationships can exist. For example, A and / or B can represent: A exists alone, A and B exist simultaneously, and B exists alone. Where A and B can be singular or plural. In this text, "at least one" means one or more, and "multiple" means two or more. "At least one kind", "at least one item (one) of the following" or similar expressions refer to any combination of these items, including any combination of single item (one) or plural items (ones). For example, "at least one item (one) of a, b, or c", or, "at least one item (one) of a, b, and c" can both represent: a, b, c, a - b (that is, a and b), a - c, b - c, or a - b - c, where a, b, and c can be single or multiple respectively.

[0091] The above are only specific embodiments of this application, enabling those skilled in the art to understand or implement this application. Various modifications to these embodiments will be obvious to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of this application. Therefore, this application will not be limited to these embodiments shown herein, but rather will conform to the broadest scope consistent with the principles and novel features claimed herein.

Claims

1. A cutting method for Cinnamomum camphora var. borneolifera, characterized in that, The cutting method comprises the following steps: Obtain Cinnamomum camphora cuttings; Insert the Cinnamomum camphora cuttings into a cutting substrate, and then conduct post-cutting management to obtain finished Cinnamomum camphora seedlings; Wherein, by weight parts, the cutting substrate comprises the following components: 40 - 60 parts of red soil, 20 - 30 parts of perlite, 15 - 20 parts of sophorolipid and 100 parts of water.

2. The cutting method of Cinnamomum camphora var. borneolifera according to claim 1, characterized in that, The sum of the weight parts of the red soil and the perlite is X, the weight part of the sophorolipid is Y, and X and Y satisfy relationship one; Relationship one: 0.20 ≤ Y / X ≤ 0.

30.

3. The cutting method of Cinnamomum camphora var. borneolifera according to claim 1, characterized in that, By weight parts, the cutting substrate comprises the following components: 42 parts of red soil, 28 parts of perlite, 18 parts of sophorolipid and 100 parts of water.

4. The cutting method of Cinnamomum camphora var. borneolifera according to claim 1, characterized in that, The sophorolipid is acidic sophorolipid.

5. The cutting method of Cinnamomum camphora var. borneolifera according to any one of claims 1 to 4, characterized in that The step of obtaining Cinnamomum camphora cuttings comprises the following process: Select ear shoots with an ear length of 2 - 5 cm, an ear diameter of 0.2 - 0.6 cm, at least 1 leaf and at least 2 nodes for shearing to obtain short shoots; Immerse the short shoots in a disinfectant for the first immersion, and then transfer them to a rooting agent for the second immersion to obtain the Cinnamomum camphora cuttings.

6. The cutting method of Cinnamomum camphora var. borneolifera according to claim 5, characterized in that, The disinfectant is composed of carbendazim and water with a weight ratio of 1:(900 - 1100); the time for the first immersion is 5 - 10 minutes.

7. The cutting method of Cinnamomum camphora var. borneolifera according to claim 5, characterized in that, The rooting agent is composed of ABT rooting powder, naphthaleneacetic acid and water with a weight ratio of 1:1:(9000 - 11000); the time for the second immersion is 1 - 2 hours.

8. The cutting method of Cinnamomum camphora var. borneolifera according to any one of claims 1 to 4, characterized in that, The cutting depth of the Cinnamomum camphora cuttings is 4 - 5 cm, and the cutting spacing is 4 - 6 cm.

9. The cutting method of Cinnamomum camphora var. borneolifera according to any one of claims 1 to 4, characterized in that, The steps of the post-cutting management include: controlling the ground temperature between 29 - 32 °C, the greenhouse temperature between 25 - 28 °C, the humidity in the greenhouse between 70% - 90%, and the shading rate in the greenhouse between 80% - 90%.

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