A skin barrier repair and cell repair anti-wrinkle composition and its preparation method

Abstract

The present invention provides a skin barrier repair and cell repair anti-wrinkle composition, which comprises 50-70 parts of component A, 10-20 parts of component B and 10-20 parts of component C by weight. The present invention constructs a liquid crystal structure and a biomimetic cortex by mixing multiple components, can effectively penetrate the stratum corneum, has high bioavailability, and the liposomes formed encapsulate the composition, has excellent stability, can simultaneously achieve barrier repair and cell repair of the epidermis and dermis, can not only effectively repair damaged cells, repair the skin barrier, but also can speed up metabolism, make cells accelerate differentiation, stimulate the production of components such as ceramide, and promote the self-barrier repair and cell repair of cells. And the ingredients used in the composition of the present invention are safe and non-irritating, and can be applied to various skin uses.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a skin barrier repair and cell repair anti-wrinkle composition and a preparation method thereof. Background Art

[0002] With the development of medicine and cosmetic science, people are increasingly aware that the core issue of skin care is whether the skin's barrier function can be better maintained. When the skin barrier function is stable, the skin will show a good health state.

[0003] However, due to various factors such as aging, stress, dry environment, etc., the skin barrier is weakened, making it more susceptible to skin diseases. As we age, the ceramides and moisture in the stratum corneum of the human skin gradually decrease.

[0004] Chinese patent CN110613663A discloses a skin repair composition, skin care product, and preparation method thereof, comprising Houttuynia cordata extract, Panax notoginseng extract, Ganoderma lucidum extract, Polygonatum odoratum extract, and American ginseng extract. The resulting skin repair composition and skin care product containing the same have hydrating, moisturizing, antibacterial, anti-inflammatory, anti-allergic, repairing, skin immunity enhancement, and anti-aging benefits. Furthermore, the skin repair solution is mild, safe, and non-toxic, suitable for all skin types. However, the composition suffers from poor stability and can only repair the epidermis.

[0005] Chinese patent CN110934788A discloses a phytoceramide synergistic skin repair composition, cosmetics, and applications thereof, comprising, by mass percentage: 0.01-1% hydrolyzed sodium hyaluronate, 0.1-5% hydrolyzed royal jelly protein, 0.1-5% glycolipid, 0.1-5% acetyl tetrapeptide-5, 0.1-5% acetyl hexapeptide-8, 0.1-5% milanocarpa leaf / stem extract, and 74-99.09% water. The provided phytoceramide synergistic skin repair composition not only achieves a synergistic effect, but also can slow down or even eliminate fine lines around the eyes, crow's feet, and facial nasolabial folds. At the same time, it can also achieve the effect of synergistic skin repair by activating cell regeneration provided by milanocarpa leaf / stem extract. However, the composition also has stability issues, cannot effectively penetrate the stratum corneum, and has low bioavailability.

[0006] Therefore, developing a product with high stability, high bioavailability, and the ability to effectively penetrate the stratum corneum while achieving barrier repair and cell repair in both the epidermis and dermis is an urgent need for current cosmetic products. Summary of the Invention

[0007] In response to the above problems, the present invention provides a skin barrier repair and cell repair anti-wrinkle composition. By mixing multiple components, a liquid crystal structure and a bionic cortex are constructed, and the liposome-encapsulated components formed have excellent stability. It can simultaneously achieve barrier repair and cell repair in the epidermis and dermis. It can not only effectively repair damaged cells and repair the skin barrier, but also accelerate metabolism, accelerate cell differentiation, stimulate the production of components such as ceramide, and promote cell self-barrier repair and cell repair.

[0008] On one hand, the present invention provides a skin barrier repair and cell repair anti-wrinkle composition. The raw materials of the composition include, by weight, 50-70 parts of component A, 10-20 parts of component B, and 10-20 parts of component C; the component A includes a surfactant, a skin conditioner, a fatty acid A, a fatty alcohol, an antioxidant, a moisturizer, and ceramide A; the component B includes phospholipids, sterols, fatty acid B, and ceramide B; and the component C includes a plant extract and a sphingolipid compound.

[0009] Preferably, the raw materials of component A include, by weight, 0.1-30 parts of surfactant, 0.1-10 parts of skin conditioner, 0.01-5 parts of fatty acid A, 0.01-5 parts of fatty alcohol, 0.01-10 parts of antioxidant, 0.1-30 parts of moisturizer, and 0.001-20 parts of ceramide A.

[0010] Further preferably, the raw materials of component A include, by weight, 5-20 parts of surfactant, 2-7 parts of skin conditioner, 0.5-3 parts of fatty acid A, 0.5-3 parts of fatty alcohol, 2-8 parts of antioxidant, 5-20 parts of moisturizer, and 5-15 parts of ceramide A.

[0011] Preferably, the surfactant is selected from one or more combinations of sodium lauroyl lactylate and hydrogenated lecithin.

[0012] Preferably, the mass ratio of the sodium lauroyl lactylate to hydrogenated lecithin is 1:0.5-1.5.

[0013] Preferably, the skin conditioning agent is dioctyldodecyl lauroyl glutamate.

[0014] Preferably, the fatty acid A is one or more combinations of myristic acid, stearic acid, and behenic acid.

[0015] Preferably, the mass ratio of the myristic acid, stearic acid and behenic acid is 1:0.5-1.5:0.5-1.5.

[0016] The fatty alcohol is one or more combinations of cetyl alcohol, stearyl alcohol and behenyl alcohol.

[0017] Preferably, the mass ratio of cetyl alcohol, stearyl alcohol and behenyl alcohol is 1:0.5-1.2:0.3-1.5.

[0018] More preferably, the mass ratio of cetyl alcohol, stearyl alcohol and behenyl alcohol is 1:1:0.5-1.

[0019] In order to further improve the stability, skin feel and long-lasting moisturizing and repairing effect of the composition, preferably, the mass ratio of the surfactant, fatty acid A and fatty alcohol is 1:0.1-0.5:0.1-0.5.

[0020] The inventors found that when a specific surfactant is compounded with fatty acid A, fatty alcohol, and ceramide A, the resulting composition has excellent stability, good skin feel, excellent moisturizing properties, and a longer-lasting effect. In particular, when the mass ratio of surfactant, fatty acid A, and fatty alcohol is 1:0.1-0.5:0.1-0.5, the best effect is achieved. The inventors analyzed that this may be because when several ingredients are compounded at specific contents, a stable liquid crystal emulsion structure can be formed in the solution, and an orderly directional arrangement can be formed at the oil-water interface. This structure is similar to the structure of human skin, which can significantly reduce the van der Waals force between scattered droplets, reduce droplet aggregation, and delay the thinning and rupture of the droplet film when the droplets meet, making the resulting combination highly stable.

[0021] At the same time, when used in combination with other ingredients in the system, the moisturizing and repairing effect of the composition is significantly enhanced. The inventors analyzed that this may be because the liquid crystal emulsified structure is a multi-layered ordered structure, which can encapsulate the active ingredients in the composition, enhancing its stability while also having a protective effect, achieving a sustained release and synergistic effect. When the product is applied to the skin surface, it can form a second skin layer similar to the skin on the skin surface, strengthening the skin barrier and effectively repairing the skin barrier; at the same time, the encapsulated active ingredients penetrate into the skin, but because the structure is a multi-layered encapsulated structure, the active ingredients are slowly released and can continue to exert their effects. Thus, a highly efficient and long-lasting moisturizing and repairing effect is achieved.

[0022] Preferably, the antioxidant is one or more combinations of squalane and phytosphingosine.

[0023] Preferably, the mass ratio of squalane to phytosphingosine is 0.5-1:0.8-1.2.

[0024] Further preferably, the mass ratio of squalane to phytosphingosine is 0.8:1.

[0025] Preferably, the moisturizing agent is one or more combinations of phytosterols and cholesterol.

[0026] Preferably, the mass ratio of the phytosterols to cholesterol is 0.1-2.5:0.1-1.

[0027] Further preferably, the mass ratio of the phytosterols to cholesterol is 0.5-1.5:0.5-1.

[0028] Preferably, the ceramide A includes one or more combinations of ceramide 1, ceramide 2, ceramide 3, ceramide 4, and ceramide 6.

[0029] Preferably, the mass ratio of ceramide 1, ceramide 2, ceramide 3, ceramide 4 and ceramide 6 is 0.1-1:0.1-2:0.1-6:0.1-1:0.1-1.

[0030] More preferably, the mass ratio of ceramide 1, ceramide 2, ceramide 3, ceramide 4, and ceramide 6 is 0.5:1:3:0.5:0.5.

[0031] Ceramide is a natural lipid that forms a waterproof barrier on the skin's surface, helping it retain moisture and promoting self-repair. The inventors discovered in experiments that by combining multiple ceramide ingredients, particularly when the mass ratio of Ceramide 1, Ceramide 2, Ceramide 3, Ceramide 4, and Ceramide 6 is 0.1-1:0.1-2:0.1-6:0.1-1:0.1-1, the resulting composition exhibits enhanced moisturizing, skin barrier repair, and anti-wrinkle effects.

[0032] Preferably, the raw materials of component B include 0.01-20 parts of phospholipids, 0.1-20 parts of sterols, 0.01-20 parts of fatty acids B, and 0.01-40 parts of ceramide B, calculated by weight.

[0033] Further preferably, the raw materials of component B include 5-15 parts of phospholipids, 5-15 parts of sterols, 5-13 parts of fatty acids B, and 10-30 parts of ceramide B, calculated by weight.

[0034] Preferably, the phospholipid is a mixture of one or more of lecithin, hexyl phytosphingosine, phytosphingosine, and acetyl phytosphingosine.

[0035] More preferably, the mass ratio of the lecithin, hexyl phytosphingosine, phytosphingosine, and acetyl phytosphingosine is 0.1-2:0.1-0.5:0.1-1:0.1-0.5.

[0036] More preferably, the mass ratio of the lecithin, hexyl phytosphingosine, phytosphingosine, and acetyl phytosphingosine is 1.5:0.3:0.5:0.3.

[0037] Preferably, the sterol is one or a combination of plant sterol and cholesterol.

[0038] Preferably, the mass ratio of the phytosterols to cholesterol is 0.5-1.5:1.

[0039] Preferably, the fatty acid B includes a mixture of one or more of linoleic acid, isomerized linoleic acid and oleic acid.

[0040] Preferably, the mass ratio of the linoleic acid, isomerized linoleic acid and oleic acid is 1:0.1-2:0.1-2.

[0041] Further preferably, the mass ratio of the linoleic acid, isomerized linoleic acid and oleic acid is 1:0.8:1.

[0042] Linoleic acid has two conjugated double bonds, which make it highly reactive. Oleic acid is a monounsaturated omega-9 fatty acid with one unsaturated double bond. When combined with several fatty acids, they effectively moisturize the skin and repair the skin barrier. The inventors unexpectedly discovered that using a mass ratio of linoleic acid, isomerized linoleic acid, and oleic acid of 1:0.1-2:0.1-2 simultaneously enhances the stability of the composition. The inventors analyze that this may be because under these conditions, the fatty acids can interact with components such as cholesterol in the system, strengthening intermolecular forces and thus enhancing the stability of the composition.

[0043] Preferably, the ceramide B is ceramide 3.

[0044] Ceramide is a very effective active ingredient, but its low solubility limits its use. Adding too much can lead to problems like precipitation and emulsion-like stratification. The present invention effectively enhances the solubility and stability of ceramide by combining it with other ingredients, significantly avoiding precipitation and stratification issues at high concentrations.

[0045] Component B is mixed with ingredients such as phospholipids, sterols, and ceramides, and multiple phospholipid components are compounded, and a high concentration of ceramide components is used at the same time to obtain a highly stable liposome-encapsulated structure, which can achieve the effects of controlled release and selective delivery. Moreover, the phytosphingosine used can also enter cells and have anti-inflammatory effects. The liposome-encapsulated components prepared by the present invention are charged liposome-encapsulated components, which not only enable the active ingredients to effectively enter the epidermal cells and achieve effective repair and maintenance of the upper epidermal cells, but also can repair damaged cells in the epidermal layer, accelerate metabolism, stimulate the granular layer and the spinous layer, secrete and produce more lipid barrier components such as ceramides, and promote cell self-repair.

[0046] Preferably, the raw materials of component C include 0.01-50 parts of plant extracts and 0.01-10 parts of sphingolipid compounds, calculated by weight.

[0047] Further preferably, the raw materials of component C include 10-30 parts of plant extracts and 2-7 parts of sphingolipid compounds, by weight.

[0048] Preferably, the plant extract comprises a mixture of one or more of hibiscus bark extract, grape flower cell extract, and sage leaf cell extract.

[0049] Preferably, the mass ratio of the hibiscus bark extract, the grape flower cell extract, and the sage leaf cell extract is 0.1-6:0.1-2:0.1-2.

[0050] Further preferably, the mass ratio of the hibiscus bark extract, the grape flower cell extract, and the sage leaf cell extract is 1-3:1-1.5:1-1.5.

[0051] Further preferably, the mass ratio of the hibiscus bark extract, the grape flower cell extract, and the sage leaf cell extract is 2:1:1.

[0052] Preferably, the sphingolipid compound includes a mixture of one or more of salicyloyl phytosphingosine and acetyl phytosphingosine.

[0053] Preferably, the mass ratio of salicyloyl phytosphingosine to acetyl phytosphingosine is 0.5-2:1.

[0054] Further preferably, the mass ratio of salicyloyl phytosphingosine to acetyl phytosphingosine is 1:1.

[0055] Component C of the present invention contains multiple plant ingredients, and through the combined use of sphingolipid compounds, a stable liposome structure containing a biphospholipid membrane is formed. The hibiscus bark extract and grape flower cell extract used have excellent antioxidant effects, and the sage leaf extract can stimulate the production of ceramides. The use of multiple plant ingredients in combination, especially when the mass ratio of the hibiscus bark extract, grape flower cell extract, and sage leaf cell extract is 1-3:1-1.5:1-1.5, can effectively accelerate skin metabolism, accelerate the differentiation of basal layer cells, and stimulate the production of ceramides, effectively achieving the repair and maintenance of the subepidermal cell layer.

[0056] Another aspect of the present invention provides a method for preparing a systemic skin barrier repair and cell repair anti-wrinkle composition, the specific steps of which are as follows:

[0057] S1, mixing a surfactant, a skin conditioning agent, a fatty acid A, a fatty alcohol, an antioxidant, a moisturizer, and a ceramide A, heating at 50-80° C., and stirring to dissolve to obtain component A;

[0058] S2, mixing phospholipids, sterols, fatty acid B, and ceramide B, heating to 50-70°C, and stirring to dissolve to obtain component B;

[0059] S3, mixing the plant extract and the sphingolipid compound, heating at 40-60° C., and stirring to dissolve to obtain component C;

[0060] S4, adding component B and component C to component A, stirring at 30-50° C. for 10-30 minutes to obtain the composition.

[0061] The systemic skin barrier repair and cell repair anti-wrinkle composition of the present invention can be applied to various cosmetic products.

[0062] Compared with the prior art, the present invention has the following beneficial effects:

[0063] The skin barrier repair and cell repair anti-wrinkle composition of the present invention, by combining multiple components to create a liquid crystal structure and biomimetic cortex, can effectively penetrate the stratum corneum, has high bioavailability, and the resulting liposome-encapsulated ingredients have excellent stability, achieving barrier repair and cell repair in both the epidermis and dermis. It not only effectively repairs damaged cells and the skin barrier, but also accelerates metabolism, accelerates cell differentiation, and stimulates the production of components such as ceramides, promoting self-barrier repair and cell repair. Furthermore, the ingredients used in the composition of the present invention are safe and non-irritating, making it suitable for use on a variety of skin types. DETAILED DESCRIPTION

[0064] The following examples are provided to further illustrate the present invention, but are not intended to limit the present invention.

[0065] Unless otherwise defined, all technical terms used hereinafter have the same meanings as those generally understood by those skilled in the art. The technical terms used herein are for the purpose of describing specific embodiments only and are not intended to limit the scope of protection of the present invention. Unless otherwise specified, the experimental materials and reagents herein are conventional commercial products in the art.

[0066] Example

[0067] Example 1

[0068] On one hand, this embodiment provides a skin barrier repair and cell repair anti-wrinkle composition, comprising 60 parts of component A, 15 parts of component B, and 15 parts of component C.

[0069] Component A, by weight, includes 5 parts of surfactant, 2 parts of skin conditioner, 0.5 parts of fatty acid A, 0.5 parts of fatty alcohol, 2 parts of antioxidant, 5 parts of moisturizer, and 5 parts of ceramide A;

[0070] The surfactant is sodium lauroyl lactylate (CAS No. 13557-75-0) and hydrogenated lecithin (CAS No. 92128-87-5) in a mass ratio of 1:1; the skin conditioning agent is dioctyldodecanol lauroyl glutamate (CAS No. 82204-94-2); the fatty acid A is myristic acid (CAS No. 544-63-8), stearic acid (CAS No. 57-11-4), and behenic acid (CAS No. 112-85-6) in a mass ratio of 1:1:1; the fatty alcohol is cetyl alcohol (CAS No. 36653-82-4), stearyl alcohol ( The ingredients are: 1) squalane (CAS No. 111-01-3) and phytosphingosine (CAS No. 13552-11-9) in a mass ratio of 0.8:1 as antioxidant; 2) phytosterols (CAS No. 83-46-5) and cholesterol (CAS No. 57-88-5) in a mass ratio of 1:1 as moisturizer; and ceramide A (CAS No. 100403-19-8) in a mass ratio of 0.5:1:3:0.5:0.5 as ceramide 1, ceramide 2, ceramide 3, ceramide 4, and ceramide 6.

[0071] Component B, by weight, includes 5 parts of phospholipids, 5 parts of sterols, 5 parts of fatty acid B, and 10 parts of ceramide B;

[0072] The phospholipids are phosphatidylcholine (CAS No. 8002-43-5), hexyl phytosphingosine, phytosphingosine (CAS No. 13552-11-9), and acetyl phytosphingosine (CAS No. 21830-28-4) in a mass ratio of 1.5:0.3:0.5:0.3; the sterols are phytosterols (CAS No. 83-46-5) and cholesterol (CAS No. 57-88-5) in a mass ratio of 1:1; the fatty acid B is linoleic acid (CAS No. 60-33-3), isomerized linoleic acid (CAS No. 60-33-3), and oleic acid (CAS No. 112-80-1) in a mass ratio of 1:0.8:1; and the ceramide B is ceramide 3.

[0073] Component C, by weight, comprises 10 parts of plant extract and 2 parts of sphingolipid compound;

[0074] The plant extracts are hibiscus (HIBISCUS SYRIACUS) bark extract, grape (VITIS VINIFERA) flower cell extract, and sage (SALVIAOFFICINALIS) leaf extract in a mass ratio of 2:1:1; the sphingolipid compounds are salicyloyl phytosphingosine (CAS No. 212908-67-3) and acetyl phytosphingosine (CAS No. 21830-28-4) in a mass ratio of 1:1.

[0075] The second aspect of this embodiment provides a method for preparing a skin barrier repair and cell repair anti-wrinkle composition, and the specific preparation steps are as follows:

[0076] S1, mixing a surfactant, a skin conditioning agent, a fatty acid A, a fatty alcohol, an antioxidant, a moisturizer, and a ceramide A, heating to 60° C., and stirring to dissolve to obtain component A;

[0077] S2, mixing phospholipids, sterols, fatty acid B, and ceramide B, heating to 60°C, and stirring to dissolve to obtain component B;

[0078] S3, mixing the plant extract and the sphingolipid compound, heating at 50° C., and stirring to dissolve to obtain component C;

[0079] S4, adding component B and component C to component A, stirring at 40° C. for 15 minutes to obtain the composition.

[0080] Example 2

[0081] On one hand, this embodiment provides a skin barrier repair and cell repair anti-wrinkle composition, comprising 60 parts of component A, 15 parts of component B, and 15 parts of component C.

[0082] Component A, by weight, includes 20 parts of a surfactant, 7 parts of a skin conditioner, 3 parts of a fatty acid A, 3 parts of a fatty alcohol, 8 parts of an antioxidant, 20 parts of a moisturizer, and 15 parts of a ceramide A; the specific raw materials selected for component A are the same as those in Example 1.

[0083] Component B, by weight, includes 15 parts of phospholipids, 15 parts of sterols, 13 parts of fatty acid B, and 30 parts of ceramide B; the specific raw materials selected for component B are the same as those in Example 1.

[0084] Component C, by weight, comprises 30 parts of plant extract and 7 parts of sphingolipid compound; the specific raw materials selected for component C are the same as those in Example 1.

[0085] The second aspect of this embodiment provides a method for preparing a skin barrier repair and cell repair anti-wrinkle composition, and the specific preparation steps are the same as those in Example 1.

[0086] Example 3

[0087] On one hand, this embodiment provides a skin barrier repair and cell repair anti-wrinkle composition, comprising 60 parts of component A, 15 parts of component B, and 15 parts of component C.

[0088] Component A, by weight, includes 10 parts of a surfactant, 5 parts of a skin conditioner, 1.5 parts of a fatty acid A, 1.5 parts of a fatty alcohol, 5 parts of an antioxidant, 12 parts of a moisturizer, and 10 parts of a ceramide A; the specific raw materials selected for component A are the same as those in Example 1.

[0089] Component B, by weight, includes 10 parts of phospholipids, 10 parts of sterols, 8 parts of fatty acid B, and 20 parts of ceramide B; the specific raw materials selected for component B are the same as those in Example 1.

[0090] Component C, by weight, includes 20 parts of plant extract and 5 parts of sphingolipid compounds; the specific raw materials selected for component C are the same as those in Example 1.

[0091] The second aspect of this embodiment provides a method for preparing a skin barrier repair and cell repair anti-wrinkle composition, and the specific preparation steps are the same as those in Example 1.

[0092] Example 4

[0093] On the one hand, this embodiment provides a skin barrier repair and cell repair anti-wrinkle composition. The specific implementation method is the same as that of Example 3. The difference from Example 3 is that the surfactant in component A is sodium lauroyl lactylate and hydrogenated lecithin in a mass ratio of 1:0.5; the fatty acid A in component A is myristic acid, stearic acid, and behenic acid in a mass ratio of 1:1.5:1.5.

[0094] The second aspect of this embodiment provides a method for preparing a skin barrier repair and cell repair anti-wrinkle composition, and the specific preparation steps are the same as those in Example 1.

[0095] Example 5

[0096] On one hand, this embodiment provides a skin barrier repair and cell repair anti-wrinkle composition, and its specific implementation is the same as that of Example 3. The difference from Example 3 is that the fatty acid A in component A is 2.5 parts.

[0097] The second aspect of this embodiment provides a method for preparing a skin barrier repair and cell repair anti-wrinkle composition, and the specific preparation steps are the same as those in Example 1.

[0098] Example 6

[0099] On the one hand, this embodiment provides a skin barrier repair and cell repair anti-wrinkle composition, and its specific implementation is the same as that of Example 3. The difference from Example 3 is that the surfactant in component A is sodium lauroyl lactylate and hydrogenated lecithin in a mass ratio of 1:1.5.

[0100] The second aspect of this embodiment provides a method for preparing a skin barrier repair and cell repair anti-wrinkle composition, and the specific preparation steps are the same as those in Example 1.

[0101] Example 7

[0102] On one hand, this embodiment provides a skin barrier repair and cell repair anti-wrinkle composition, and its specific implementation is the same as that of Example 3. The difference from Example 3 is that the amount of ceramide 3 in component B is 30 parts.

[0103] The second aspect of this embodiment provides a method for preparing a skin barrier repair and cell repair anti-wrinkle composition, and the specific preparation steps are the same as those in Example 1.

[0104] Example 8

[0105] On one hand, this embodiment provides a skin barrier repair and cell repair anti-wrinkle composition, and its specific implementation is the same as that of Example 3. The difference from Example 3 is that the plant extract in component C is 30 parts.

[0106] The second aspect of this embodiment provides a method for preparing a skin barrier repair and cell repair anti-wrinkle composition, and the specific preparation steps are the same as those in Example 1.

[0107] Comparative Example 1

[0108] On one hand, this embodiment provides a skin barrier repair and cell repair anti-wrinkle composition, and its specific implementation is the same as that of Example 3. The difference from Example 3 is that the amount of ceramide 3 in component B is 5 parts.

[0109] The second aspect of this embodiment provides a method for preparing a skin barrier repair and cell repair anti-wrinkle composition, and the specific preparation steps are the same as those in Example 1.

[0110] Comparative Example 2

[0111] On one hand, this embodiment provides a skin barrier repair and cell repair anti-wrinkle composition, and its specific implementation is the same as that of Example 3. The difference from Example 3 is that the plant extract in component B is 5 parts.

[0112] The second aspect of this embodiment provides a method for preparing a skin barrier repair and cell repair anti-wrinkle composition, and the specific preparation steps are the same as those in Example 1.

[0113] Comparative Example 3

[0114] On the one hand, this embodiment provides a skin barrier repair and cell repair anti-wrinkle composition, and its specific implementation is the same as that of Example 3. The difference from Example 3 is that the fatty acid A in component A is 8 parts and the fatty alcohol is 8 parts.

[0115] The second aspect of this embodiment provides a method for preparing a skin barrier repair and cell repair anti-wrinkle composition, and the specific preparation steps are the same as those in Example 1.

[0116] Comparative Example 4

[0117] On one hand, this embodiment provides a skin barrier repair and cell repair anti-wrinkle composition, and its specific implementation is the same as that of Example 3. The difference from Example 3 is that the fatty acid B in component B is 3 parts.

[0118] The second aspect of this embodiment provides a method for preparing a skin barrier repair and cell repair anti-wrinkle composition, and the specific preparation steps are the same as those in Example 1.

[0119] Comparative Example 5

[0120] On one hand, this embodiment provides a skin barrier repair and cell repair anti-wrinkle composition, and its specific implementation is the same as that of Example 3. The difference from Example 3 is that the phospholipid in component B is 3 parts.

[0121] The second aspect of this embodiment provides a method for preparing a skin barrier repair and cell repair anti-wrinkle composition, and the specific preparation steps are the same as those in Example 1.

[0122] Performance Testing

[0123] 1. Repair effect test

[0124] The sample of Example 1 was tested for repair effect, and the repair effect was evaluated using the T / GDCA009-2022 cosmetic repair efficacy human evaluation method.

[0125] Subjects: 32 subjects aged 18-45 years were selected, with a basic facial stratum corneum moisture content of <60 a.u., rough facial skin, poor skin barrier, cheek transepidermal water loss value >12g / h, facial redness symptoms, and self-perception that their skin is prone to one or more of the following reactions: redness, itching, heat, and pain. They also had normal skin, excessive oil secretion on the forehead, and dry and dehydrated cheeks.

[0126] Using a product versus blank control method, the transepidermal water loss (TEWL) and stratum corneum moisture content of the test area were measured before, 6 hours after, and 8 hours after product use. Using a before-after control method, the TEWL and TEW content of the test area were measured before, 7 days after, and 14 days after continuous product use. The results are shown in Tables 1 and 2 below.

[0127] Table 1 Transepidermal water loss values

[0128] sample Base Value After 6 hours of use After 8 hours of use After 7 days of use After 14 days of use Example 1 23.21±0.63 19.24±0.67 18.98±0.74 19.45±0.71 17.96±0.71 Blank control 22.76±0.63 21.09±0.66 20.94±0.71 / /

[0129] Table 2 Improvement rate of moisture content in skin stratum corneum

[0130] sample After 6 hours of use After 8 hours of use After 7 days of use After 14 days of use Example 1 81.25％ 70.23％ 36.41％ 59.33％ Blank control 0.67％ 0.03％ / /

[0131] The results showed that the change in transepidermal water loss (TEWL) values 6 and 8 hours after a single use significantly decreased compared to the blank control area (p<0.001). The change in stratum corneum moisture content (TEWC) values 6 and 8 hours after a single use significantly increased compared to the blank control area (p<0.001). This product has a moisturizing effect that lasts for 6 and 8 hours. After 7 consecutive days of use, the TEWL values of the test area significantly decreased compared to the baseline value (0.01≤p<0.05); the stratum corneum moisture content of the test area significantly increased compared to the baseline value (p<0.001). After 14 consecutive days of use, the TEWL values of the test area significantly decreased compared to the baseline value (p<0.001); the stratum corneum moisture content of the test area significantly increased compared to the baseline value (p<0.001). This combination has moisturizing and repairing effects.

[0132] 2. Anti-wrinkle performance test

[0133] Anti-wrinkle efficacy was evaluated using a laboratory method (HMC-W1-028 elastase inhibition rate). Elastase, primarily synthesized and secreted by fibroblasts, degrades elastin in the skin, contributing to skin aging. The experimental principle for elastase inhibition is that porcine pancreatic elastase catalyzes a reaction with the enzyme substrate. Adding the active substance causes a change in absorbance, which reflects the inhibition rate of the elastase inhibitor.

[0134] Test method:

[0135] The sample was diluted with pure water to a sample concentration of 1%; the positive control (EGCG) was diluted with water to a positive control concentration of 0.1%; the negative control was purified water.

[0136] Set up sample group, sample background group, solvent group and solvent background group. Each group needs to set up 3 parallels. Add different reagent solutions into the 96-well plate, shake gently, incubate at 25℃ for 15 minutes, place it in the microplate reader, and measure the absorbance at 410nm.

[0137] Elastase inhibition rate (%) = (1-(CD / AB)) × 100

[0138] Wherein, A is the absorbance of the reaction solution without sample; B is the absorbance of the reaction solution without sample and enzyme; C is the absorbance of the reaction solution with sample and enzyme; D is the absorbance of the reaction solution with sample and no enzyme.

[0139] This test refers to the laboratory method (HMC-W1-028 elastase inhibition rate) and compares the elastase inhibition rate test results of the test sample with that of the negative control. If the inhibition rate of the test sample is higher than that of the negative control and the difference is significant (P<0.05), it can be considered that the test sample has certain anti-wrinkle efficacy.

[0140] The sample of Example 1 was tested for its repair effect. The test results showed that the positive control elastase inhibition rate was >50%, indicating that the reaction system was effective. When the sample was diluted into a 1% aqueous solution, the elastase inhibition rate was 45.196%, which was significantly different from the negative control (P < 0.05), indicating that the sample had anti-wrinkle effect.

[0141] 3. Stability test

[0142] The skin barrier repair and cell repair anti-wrinkle composition samples of Examples 1-7 and Comparative Examples 1-5 were packaged in colorless, transparent plastic wide-mouth bottles (not exposed). The bottles were placed at -10°C, 0°C, 25°C, and 45°C, and under light conditions (room temperature 25°C) for 2 months, and their appearance was continuously observed. The degree of change was represented by a rating of A\B\C\D, where A indicates no significant change in the sample, B indicates an acceptable slight change (such as a slight change in viscosity), C indicates an unacceptable change (such as slight discoloration or delamination), and D indicates a severe change (such as discoloration, delamination, or crystal precipitation). The test results are shown in Table 3.

[0143] Table 3

[0144]

[0145]

Claims

1. A skin barrier repair and cell repair anti-wrinkle composition, characterized in that: In parts by weight, the raw materials of the composition include 50-70 parts of component A, 10-20 parts of component B, and 10-20 parts of component C; The raw materials of component A include, by weight, 0.1-30 parts of a surfactant, 0.1-10 parts of a skin conditioner, 0.01-5 parts of a fatty acid A, 0.01-5 parts of a fatty alcohol, 0.01-10 parts of an antioxidant, 0.1-30 parts of a moisturizer, and 0.001-20 parts of a ceramide A; the fatty acid A is a combination of one or more of myristic acid, stearic acid, and behenic acid; The raw materials of component B include 5-15 parts of phospholipids, 5-15 parts of sterols, 5-13 parts of fatty acid B, and 10-30 parts of ceramide B by weight; the fatty acid B includes a mixture of one or more of linoleic acid, isomerized linoleic acid, and oleic acid; In parts by weight, the raw materials of component C include 0.01-50 parts of plant extracts and 0.01-10 parts of sphingolipid compounds.

2. The skin barrier repair and cell repair anti-wrinkle composition according to claim 1, characterized in that: The mass ratio of the surfactant, fatty acid A and fatty alcohol is 1:0.1-0.5:0.1-0.

5.

3. The skin barrier repair and cell repair anti-wrinkle composition according to claim 1, characterized in that: The ceramide A includes one or more combinations of ceramide 1, ceramide 2, ceramide 3, ceramide 4, and ceramide 6.

4. The skin barrier repair and cell repair anti-wrinkle composition according to claim 1, characterized in that: The phospholipid is a mixture of one or more of lecithin, hexyl phytosphingosine, phytosphingosine, and acetyl phytosphingosine.

5. The skin barrier repair and cell repair anti-wrinkle composition according to claim 1, characterized in that: The mass ratio of the lecithin, hexyl phytosphingosine, phytosphingosine and acetyl phytosphingosine is 0.1-2:0.1-0.5:0.1-1:0.1-0.

5.

6. The skin barrier repair and cell repair anti-wrinkle composition according to claim 1, characterized in that: The plant extract comprises a mixture of one or more of hibiscus bark extract, grape flower cell extract, and sage leaf cell extract.

7. The method for preparing the systemic skin barrier repair and cell repair anti-wrinkle composition according to any one of claims 1 to 6, characterized in that: The specific steps are as follows: S1, mixing a surfactant, a skin conditioning agent, a fatty acid A, a fatty alcohol, an antioxidant, a moisturizer, and a ceramide A, heating at 50-80° C., and stirring to dissolve to obtain component A; S2, mixing phospholipids, sterols, fatty acid B, and ceramide B, heating to 50-70°C, and stirring to dissolve to obtain component B; S3, mixing the plant extract and the sphingolipid compound, heating at 40-60° C., and stirring to dissolve to obtain component C; S4, adding component B and component C to component A, stirring at 30-50° C. for 10-30 minutes to obtain the composition.

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