A composition and its preparation method and application

Abstract

The present invention discloses a composition, a preparation method, and an application thereof. The composition comprises the following raw materials: folic acid, zinc ions, and niacinamide, wherein the molar ratio of folic acid, zinc ions, and niacinamide is 1:0.5:0.1-1:1.5:10; or the composition comprises the following raw materials: folic acid, zinc ions, and total glycosides of Centella asiatica, wherein the molar ratio of folic acid, zinc ions, and total glycosides of Centella asiatica is 1:0.5:0.1-1:1.5:5. The advantages of this application are: 1) the composition of this application has a synergistic effect through folic acid, zinc ions, and niacinamide, thereby increasing the permeability of folic acid and niacinamide at the same time; 2) the folic acid and zinc ions in the composition of this application form a three-dimensional network structure, which can enhance the stability of folic acid and maintain the effective ingredients in the composition in a stable state; 3) when used in cosmetics, the composition of this application has the effects of moisturizing, promoting the repair of damaged DNA, whitening, and brightening the skin tone.

Description

[0001] This application claims priority to the Chinese patent application filed with the Intellectual Property Office of the People's Republic of China on May 5, 2023, with application number 2023105108435 and invention name "A composition, its preparation method and application", the entire contents of which are incorporated by reference into this application. Technical Field

[0002] The present disclosure relates to the technical field of cosmetics, and in particular to a composition, a preparation method and an application thereof. Background Art

[0003] Folic acid, an essential B vitamin required by the human body, moisturizes and promotes DNA repair, and is widely used in cosmetics. Folic acid has low solubility and is often dissolved in alkaline solutions to increase its solubility. However, folic acid solutions are unstable and easily decompose and precipitate under light.

[0004] Niacinamide is also a B vitamin and is widely used in whitening cosmetics. It can reduce the production and precipitation of melanin, block the migration of melanin to the epidermis, play a role in whitening and brightening the skin tone, and also has a certain effect in promoting stratum corneum metabolism and anti-aging.

[0005] However, niacinamide has a low transdermal permeability, and this is often enhanced by the addition of permeation enhancers, such as ethanol, propylene glycol, azone, organic acids, and surfactants. However, the use of permeation enhancers in cosmetics not only enhances the permeation of active ingredients but also strongly enhances the permeation of pigments, fragrances, and preservatives, leading to caution in their practical application. Therefore, while niacinamide is often added to cosmetics for whitening, brightening, promoting stratum corneum metabolism, and anti-aging benefits, its low permeability prevents it from being absorbed by the skin, resulting in the vast majority of the product being wasted and failing to achieve the claimed effects.

[0006] Similar to niacinamide, Centella asiatica extract is also widely used in cosmetics. Centella asiatica extract has the functions of moisturizing, maintaining the water-oil balance of the skin, and delaying aging, but the skin's absorption rate of Centella asiatica extract is not high. Summary of the Invention

[0007] The present disclosure provides a composition, a preparation method and an application thereof, to solve at least one of the technical problems existing in the prior art.

[0008] According to a first aspect of the present disclosure, a composition is provided.

[0009] A composition comprising the following raw materials: folic acid, zinc ions and nicotinamide, wherein the molar ratio of folic acid, zinc ions and nicotinamide is 1:0.5:0.1-1:1.5:10;

[0010] Alternatively, the composition comprises the following raw materials: folic acid, zinc ions and total glycosides of Centella asiatica, and the molar ratio of folic acid, zinc ions and total glycosides of Centella asiatica is 1:0.5:0.1-1:1.5:5.

[0011] In one embodiment, the molar ratio of folic acid, zinc ion and nicotinamide is 1:0.8:1-1:1.2:5;

[0012] The molar ratio of folic acid, zinc ion and total glycosides of Centella asiatica is 1:0.5:0.5-1:1.5:2.

[0013] In one embodiment, the molar ratio of folic acid, zinc ion and nicotinamide is 1:1:3.6;

[0014] The molar ratio of folic acid, zinc ion and nicotinamide is 1:0.5:1;

[0015] The molar ratio of folic acid, zinc ion and nicotinamide is 1:1:1;

[0016] The molar ratio of folic acid, zinc ion and nicotinamide is 1:1:2;

[0017] The molar ratio of folic acid, zinc ion and nicotinamide is 1:1:5;

[0018] The molar ratio of folic acid, zinc ion and total glycosides of Centella asiatica is 1:1:0.46.

[0019] In one embodiment, the zinc ion is a soluble zinc salt, which includes zinc sulfate, zinc chloride, zinc gluconate, zinc acetate, zinc lactate, and amino acid zinc; and the nicotinamide is nicotinamide and / or a nicotinamide derivative.

[0020] According to a second aspect of the present disclosure, a method for preparing a composition is provided, wherein the first method comprises the following steps: Step 1) Preparation of Solution A: fully dissolving folic acid in an alkaline solution;

[0021] Step 2): Preparation of solution B: Dissolve zinc ions in water;

[0022] Step 3): Preparation of solution C: dissolving nicotinamide in water;

[0023] Step 4): mixing solution A and solution B, stirring, adding solution C, and maintaining the pH value at 8-9 to finally obtain the composition;

[0024] Alternatively, method 2 comprises the following steps: 1) preparing solution A: fully dissolving folic acid in an alkaline solution;

[0025] Step II): Preparation of solution B: Dissolve zinc ions in water;

[0026] Step III): Preparation of Solution C: Dissolve total glycosides of Centella asiatica in water;

[0027] Step IV): mixing solution A and solution B, stirring, adding solution C, and maintaining the pH value at 8-9 to finally obtain the composition.

[0028] In one embodiment, the alkaline solution in both method 1 and method 2 comprises one of an inorganic alkaline solution, an organic alkaline solution and an alkaline buffer solution.

[0029] In one embodiment, the alkaline solution includes a buffer solution of sodium hydroxide, potassium hydroxide, sodium carbonate, sodium bicarbonate, triethanolamine, and tromethamine.

[0030] In one embodiment, in method 1 and method 2, the mass content of folic acid in the prepared composition is 0.01-10%.

[0031] In one embodiment, the mass content of folic acid in the prepared composition is 0.1-5%.

[0032] According to the third aspect of the present disclosure, the present disclosure also provides an application of the composition, wherein the composition is applied to cosmetics; the cosmetics are cosmetics that have the functions of moisturizing, promoting damaged DNA repair, whitening, brightening skin tone, and anti-aging.

[0033] In this application, folic acid and zinc ions form a supramolecular assembly structure in solution through hydrogen bonds and π-π bonds, which improves stability and achieves a transdermal permeability of 75%. Nicotinamide is introduced into the folic acid and zinc ion supramolecular system to prepare a composition containing folic acid and nicotinamide, which has a synergistic effect with nicotinamide, increasing the permeability of nicotinamide by 30%.

[0034] This is because folic acid has a pteridine structure at one end and a glutamic acid structure at the other end, as shown in Formula 1.

[0035]

[0036] The pteridine end is hydrophobic and lipophilic, and the four pteridine rings form a quadruplex sheet structure through hydrogen bonds, such as Figure 1 As shown, the lamellar structures are connected by π-π bonds to form filaments. Under the comprehensive control of pH and zinc ions, the glutamate groups at the outer ends of the filaments combine with zinc ions to form a three-dimensional network structure, which improves the stability of folic acid. The viscosity of the system increases with the increase of folic acid concentration. Figure 2 shown.

[0037] The principle of synergistic enhancement is to utilize the hydrophobic and lipophilic properties of the pteridine end of folic acid to combine with the pyridine end of niacinamide in a similar and compatible manner, encapsulating them in a three-dimensional system. In transdermal testing, the high permeability of folic acid itself synergistically enhances the penetration of niacinamide. The formula of niacinamide is shown in Formula 2.

[0038]

[0039] Similar to the above situation, folic acid and zinc ions form a supramolecular assembly structure through hydrogen bonds and π-π bonds in the solution, and the stability is improved. Then, total Centella asiatica glucoside is introduced, and folic acid and zinc ions play a synergistic role with total Centella asiatica glucoside, thereby improving the absorption rate of total Centella asiatica glucoside.

[0040] Compared with the prior art, the advantages of the present application are: 1) the composition prepared by the present application has a synergistic effect through folic acid, zinc ions and niacinamide, and the permeability of niacinamide is improved while the permeability of folic acid is improved; 2) after the folic acid and zinc ions in the composition of the present application form a three-dimensional network structure, the stability of folic acid can be improved, so that the effective ingredients in the composition remain in a stable state; 3) while the folic acid in the composition prepared by the present application has a high permeability of 75%, the transdermal permeability of niacinamide is increased by at least 30%; 4) in the composition prepared by the present application, folic acid, zinc ions and total glycosides of Centella asiatica have a synergistic effect, which improves the absorption rate of total glycosides of Centella asiatica, thereby improving the absorption rate of Centella asiatica extract; 5) both compositions of the present application can be used in cosmetics, and have the effects of moisturizing, promoting the repair of damaged DNA, whitening and brightening the skin tone.

[0041] It should be understood that the contents described in this section are not intended to identify the key or important features of the embodiments of the present disclosure, nor are they intended to limit the scope of the present disclosure. Other features of the present disclosure will become readily understood through the following description. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] The above and other objects, features and advantages of the exemplary embodiments of the present disclosure will become readily understood by reading the detailed description below with reference to the accompanying drawings, in which several embodiments of the present disclosure are shown by way of example and not limitation, wherein:

[0043] In the drawings, the same or corresponding reference numerals denote the same or corresponding parts.

[0044] Figure 1 A schematic diagram showing the formation of a quadruplex between folic acid molecules and metal zinc ions in an embodiment of the present disclosure is shown;

[0045] Figure 2 A schematic diagram showing the formation of fibers after folic acid molecules and metal zinc ions form a quadruplex in an embodiment of the present disclosure;

[0046] Figure 3 A schematic diagram showing the test of nicotinamide permeability of the composition prepared in Example 1 of the present disclosure is shown;

[0047] Figure 4 A schematic diagram showing the cumulative permeation amount of total Centella asiatica glycosides in the sample group and the control group of Example 2-1 of the present disclosure is shown;

[0048] Figure 5 A schematic diagram showing the diffusion percentage of total Centella asiatica glycosides in the sample group and the control group of Example 2-1 of the present disclosure is shown;

[0049] Figure 6 A schematic diagram of the permeation rates of the sample group and the control group of Example 2-1 of the present disclosure is shown. DETAILED DESCRIPTION

[0050] To make the purposes, features, and advantages of the present disclosure more apparent and understandable, the technical solutions in the embodiments of the present disclosure will be clearly and completely described below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present disclosure, not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of the present disclosure without creative work shall fall within the scope of protection of the present disclosure.

[0051] The raw materials used in this application are all commercially available products.

[0052] In a first aspect, the present application provides a composition comprising the following raw materials: folic acid, zinc ions, and niacinamide, wherein the molar ratio of folic acid, zinc ions, and niacinamide is 1:0.5:0.1 to 1:1.5:10. Preferably, the molar ratio of folic acid, zinc ions, and niacinamide is 1:0.8:1 to 1:1.2:5. More preferably, the molar ratio of folic acid, zinc ions, and niacinamide is 1:1:3.6; the molar ratio of folic acid, zinc ions, and niacinamide is 1:0.5:1; the molar ratio of folic acid, zinc ions, and niacinamide is 1:1:1; the molar ratio of folic acid, zinc ions, and niacinamide is 1:1:2; and the molar ratio of folic acid, zinc ions, and niacinamide is 1:1:5.

[0053] The zinc ion is a soluble zinc salt, including zinc sulfate, zinc chloride, zinc gluconate, zinc acetate, zinc lactate, and zinc amino acid; and the niacinamide is niacinamide and / or a niacinamide derivative. This combination achieves a high permeability of folic acid of 75% and increases the transdermal permeability of niacinamide by over 30%, thereby enhancing the efficacy of folic acid and niacinamide in cosmetics.

[0054] The present application further provides a composition comprising the following raw materials: folic acid, zinc ions, and asiaticoside, wherein the molar ratio of folic acid, zinc ions, and asiaticoside is 1:0.5:0.1 to 1:1.5:5. Preferably, the molar ratio of folic acid, zinc ions, and asiaticoside is 1:0.5:0.5 to 1:1.5:2. More preferably, the molar ratio of folic acid, zinc ions, and asiaticoside is 1:1:0.46.

[0055] Centella asiatica extract is generally extracted from Centella asiatica, and the Centella asiatica extract includes asiaticoside, madecassoside, and other components. In this application, the total glycosides of Centella asiatica account for 80% by weight of the Centella asiatica extract. The total glycosides of Centella asiatica include asiaticoside and madecassoside, with asiaticoside accounting for 20% by weight of the Centella asiatica extract and madecassoside accounting for 60% by weight of the Centella asiatica extract.

[0056] In a second aspect, the present application further provides a method for preparing the above-mentioned composition, comprising the following steps: Step 1), preparing solution A: fully dissolving folic acid in an alkaline solution (the amount of alkaline solution used is sufficient to completely dissolve the folic acid);

[0057] Step 2), preparation of solution B: dissolving zinc ions in water;

[0058] Step 3), preparation of solution C: dissolving nicotinamide solution in water;

[0059] Step 4): Solution A and solution B are mixed and stirred to prepare a folic acid zinc ion hydrogel. The stirring is continued, and solution C is added and stirred evenly. The pH value is maintained at 8-9 (if the pH value is not reached, it can be adjusted with an alkaline solution) to obtain a composition.

[0060] The alkaline solution includes one of an inorganic alkaline solution, an organic alkaline solution and an alkaline buffer solution. Specifically, the alkaline solution includes a buffer solution of sodium hydroxide, potassium hydroxide, sodium carbonate, sodium bicarbonate, triethanolamine and tromethamine.

[0061] The mass content of folic acid is 0.01-10%, preferably 0.1-5%, and more preferably 0.3%, 1% and 3%.

[0062] The present application further provides a method for preparing the above-mentioned composition, comprising the following steps: Step I), preparing solution A: fully dissolving folic acid in an alkaline solution (the amount of alkaline solution used is sufficient to completely dissolve the folic acid);

[0063] Step II), preparation of solution B: dissolving zinc ions in water;

[0064] Step III), preparation of solution C: dissolving total glycosides of Centella asiatica in water;

[0065] Step IV): Solution A and Solution B are mixed and stirred to prepare a folic acid zinc ion hydrogel. The stirring is continued, and Solution C is added and stirred evenly. The pH value is maintained at 8-9 (if the pH value is not reached, it can be adjusted with an alkaline solution) to obtain a composition.

[0066] The alkaline solution includes one of an inorganic alkaline solution, an organic alkaline solution and an alkaline buffer solution. Specifically, the alkaline solution includes a buffer solution of sodium hydroxide, potassium hydroxide, sodium carbonate, sodium bicarbonate, triethanolamine and tromethamine.

[0067] The mass content of folic acid is 0.01-10%. Preferably, the mass content of folic acid is 0.1-5%. More preferably, the mass content of folic acid is 0.3%, 1% and 3%.

[0068] Thirdly, both compositions of this application can also be used in cosmetics. The first composition, a folic acid-zinc ion-niacinamide composition, can be used in cosmetics such as serums, lotions, and creams, offering moisturizing, promoting damaged DNA repair, whitening, brightening skin tone, and anti-aging benefits. The second composition, a folic acid-zinc ion-Centella asiatica total glycosides composition, can be used in cosmetics such as serums, lotions, and creams, offering hydration, moisturizing, maintaining skin water-oil balance, and delaying aging.

[0069] In the conventional preparation of cosmetics, folic acid and metallic zinc ions are generally not used simultaneously, and they are compounded with emulsifiers or moisturizers to prepare cosmetics. This is because metallic zinc ions will push folic acid out of the solution, causing folic acid to form a precipitate, thereby reducing the effective ingredients in the cosmetics.

[0070] The present application first dissolves folic acid in an alkaline solution and prepares a stable composition with metal zinc ions and niacinamide. The folic acid, metal zinc ions and niacinamide are all encapsulated in a three-dimensional system. This composition with a stable three-dimensional system is compounded with an emulsifier or moisturizer or other mild functional ingredients required for preparing cosmetics. The addition of other raw materials will not destroy the structure of the three-dimensional system. Therefore, while the folic acid in the composition has a high permeability of 75%, the transdermal permeability of niacinamide is increased by 30%, allowing the efficacy of folic acid and niacinamide in cosmetics to be better exerted.

[0071] Similarly, the applicant discovered that compounding folic acid, zinc ions and asiatica total glucosides together also has a synergistic effect, increasing the penetration rate of asiatica total glucosides by 57.6%, thereby better exerting the efficacy of asiatica total glucosides in cosmetics.

[0072] (1) Embodiment

[0073] A method for preparing a composition comprises the following steps:

[0074] Step 1) Preparation of Solution A: Fully dissolve folic acid in an appropriate amount of sodium hydroxide solution (the amount of sodium hydroxide used is sufficient to completely dissolve the folic acid);

[0075] Step 2), preparation of solution B: dissolving zinc ions in water;

[0076] Step 3), preparation of solution C: dissolving nicotinamide solution in water;

[0077] Step 4): Solution A and solution B were mixed and stirred to prepare a folic acid zinc ion hydrogel. The stirring was continued, and solution C was added and stirred evenly. The balance was supplemented with water, and the pH value was maintained at 8-9 to obtain a composition with a total mass of 100 g.

[0078] Please refer to Table 1 below for specific preparation examples.

[0079] Table 1

[0080]

[0081]

[0082] The composition prepared in Example 1 of this application was tested for the diffusion rate of folic acid and niacinamide, as well as the permeation rate of niacinamide. The primary equipment required for this testing was a TK-12D transdermal absorption diffusion instrument (Shanghai Kaikai) and a Franz cell diffusion cell (Shanghai Kaikai). The test was conducted in accordance with GB / T 27818-2011, In Vitro Test Method for Skin Absorption of Chemicals.

[0083] The diffusion rate formula is: P = Q / P0x100%, where P: diffusion percentage, Q: cumulative permeation volume, and P0: initial sample load in the diffusion chamber.

[0084] Cumulative permeation amount Q = [Cn×V0+∑Ci×Vi], where Q is the cumulative permeation amount, V0 is the volume of the receiving liquid in the receiving chamber, Vi is the volume of each sampling, Ci is the concentration of the target substance in the receiving liquid from the 1st to the n-1th sampling time, and Cn is the concentration of the target substance measured at the nth sampling point.

[0085] The test is as follows:

[0086] (I) The composition prepared in Example 1 was subjected to a folic acid diffusion rate test (using suckling pig skin close to human skin, and tested according to the existing test method). The test results are shown in Table 2.

[0087] Table 2

[0088]

[0089] As can be seen from Table 2, the folic acid diffusion rate (permeability) in the composition prepared in Example 1 reaches 75%.

[0090] (II) The composition prepared in Example 1 was tested for the diffusion percentage of niacinamide, wherein the molar content of niacinamide in the sample group was the same as that in the control group of Example 1. The diffusion percentage results are shown in Table 3 below.

[0091] Table 3

[0092]

[0093] As can be seen from Table 3, the diffusion percentage of nicotinamide in the sample group was 12.50% at 24 hours, while the diffusion percentage of nicotinamide in the control group of Example 1 of the present application reached 16.29%.

[0094] (III) The composition prepared in Example 1 was tested for its nicotinamide penetration (using suckling pig skin close to human skin and tested according to the existing test method). The test results are as follows: Figure 3 As shown. The sample group contains only nicotinamide, and the molar content of nicotinamide is the same as that of nicotinamide in Example 1. The control group is the composition prepared in Example 1. Figure 3 It can be seen that the cumulative permeation amount of the sample group in 24 hours is 0.15 mg, and the cumulative permeation amount of the control group in Example 1 of the present application in 24 hours is 0.20 mg. Compared with the sample group, the permeability of nicotinamide in the composition of Example 1 of the present application is increased by 33.3%.

[0095] (2) Example

[0096] A method for preparing a composition comprises the following steps:

[0097] Step I), preparation of solution A: fully dissolving folic acid in an appropriate amount of sodium hydroxide solution (the amount of sodium hydroxide used is sufficient to completely dissolve the folic acid);

[0098] Step II), preparation of solution B: dissolving zinc ions in water;

[0099] Step III), preparation of solution C: dissolving the total Centella asiatica solution in water;

[0100] Step IV): Solution A and Solution B were mixed and stirred to prepare a folic acid zinc ion hydrogel. The stirring was continued, and Solution C was added and stirred evenly. The balance was supplemented with water. The pH value was maintained at 8-9 to obtain a composition with a total mass of 100 g.

[0101] Please refer to Table 4 below for specific preparation examples.

[0102] Table 4

[0103]

[0104] The composition prepared in Example 2-1 of the present application was subjected to a diffusivity test of total asiaticoside and a permeability test of total asiaticoside. The tests are as follows:

[0105] (I) The cumulative penetration of total Centella asiatica glycosides in the composition prepared in Example 2-1 was tested (using suckling pig skin which is close to human skin). The test results are shown in Tables 5 and 6.

[0106] Among them, Table 5 shows the initial content of total glycosides of Centella asiatica in the control group and the sample group

[0107] Table 5 Initial content

[0108] Sample name Content of total glycosides in Centella asiatica (%) Control group: Centella asiatica extract 0.3 Sample group: Example 2-1 composition 0.3

[0109] Table 6 Cumulative permeation results of samples

[0110]

[0111] Among them, Table 5 shows the initial content values ​​of the sample group (the composition prepared in Example 2-1) and the control group (Centella asiatica extract). It can be seen from Table 5 that the total glycosides content of Centella asiatica is the same. Then, according to the cumulative permeation formula, the cumulative permeation of the samples at different time points was obtained. The volume of each sampling was 1.0 ml. The test results are shown in Table 6. It can be seen from Table 6 that the cumulative permeation of the control group for 24 hours was 0.026, while the cumulative permeation of the total glycosides of Centella asiatica in the sample group of this application for 24 hours was 0.041. The test diagram is as follows Figure 4 shown.

[0112] (2) According to the diffusion percentage formula, the composition of Example 2-1 was tested to obtain the diffusion percentage of the sample at different time points. The results are shown in Table 7. The test graph is shown in Figure 5 shown.

[0113] Table 7 Diffusion percentage results

[0114]

[0115] The cumulative permeation volume was used to make a regression equation for the permeation time. The slope of the equation was the permeability coefficient Kp (i.e., the permeation rate). The results are shown in Table 8 and Figure 6 shown.

[0116] Table 8 Permeation rate results

[0117]

[0118] In summary, the control group (Centella asiatica extract) underwent transdermal absorption testing, and the 24-hour permeability of total asiatica glycosides was 2.17%. The sample group (composition prepared in Example 2-1 of the present application) underwent transdermal absorption testing, and the 24-hour permeability of total asiatica glycosides was 3.42%. Compared with the control group (Centella asiatica extract), the 24-hour permeability of total asiatica glycosides in the sample group increased by 57.6%. This indicates that the folic acid and zinc in the present application synergistically promote the transdermal absorption of total asiatica glycosides.

Claims

1. A composition, characterized in that: The invention comprises the following raw materials: folic acid, zinc ion and nicotinamide, and the molar ratio of folic acid, zinc ion and nicotinamide is 1:0.5:0.1-1:1.5:10; The zinc ion is a soluble zinc salt, which includes zinc sulfate, zinc chloride, zinc gluconate, zinc acetate, zinc lactate, and amino acid zinc; the nicotinamide is nicotinamide and / or a nicotinamide derivative; The preparation method of the composition comprises the following steps: 1) preparing solution A: fully dissolving folic acid in an alkaline solution; Step 2): Preparation of solution B: Dissolve zinc ions in water; Step 3): Preparation of solution C: dissolving nicotinamide in water; Step 4): Solution A and solution B are mixed, stirred, and solution C is added, and the pH value is maintained at 8-9 to finally obtain the composition.

2. A composition according to claim 1, characterized in that: The molar ratio of folic acid, zinc ion and nicotinamide is 1:0.8:1-1:1.2:

5.

3. A composition according to claim 1, characterized in that: The molar ratio of folic acid, zinc ion and nicotinamide is 1:1:3.6; The molar ratio of folic acid, zinc ion and nicotinamide is 1:0.5:1; The molar ratio of folic acid, zinc ion and nicotinamide is 1:1:1; The molar ratio of folic acid, zinc ion and nicotinamide is 1:1:2; The molar ratio of folic acid, zinc ion and nicotinamide is 1:1:

5.

4. A composition according to claim 1, characterized in that: The alkaline solution includes one of an inorganic alkaline solution, an organic alkaline solution and an alkaline buffer solution.

5. A composition according to claim 4, characterized in that: The alkaline solution includes a series of buffer solutions of sodium hydroxide, potassium hydroxide, sodium carbonate, sodium bicarbonate, triethanolamine and tromethamine.

6. A composition according to claim 1, characterized in that: In the prepared composition, the mass content of folic acid is 0.01-10%.

7. A composition according to claim 6, characterized in that: In the prepared composition, the mass content of folic acid is 0.1-5%.

8. A composition, characterized in that: The composition comprises the following raw materials: folic acid, zinc ions and total glycosides of Centella asiatica, and the molar ratio of folic acid, zinc ions and total glycosides of Centella asiatica is 1:0.5:0.1-1:1.5:5; The zinc ion adopts a soluble zinc salt, and the soluble zinc salt includes zinc sulfate, zinc chloride, zinc gluconate, zinc acetate, zinc lactate, and amino acid zinc; The preparation method of the composition comprises the following steps: 1) preparing solution A: fully dissolving folic acid in an alkaline solution; Step II): Preparation of solution B: Dissolve zinc ions in water; Step III): Preparation of Solution C: Dissolve total glycosides of Centella asiatica in water; Step IV): mixing solution A and solution B, stirring, adding solution C, and maintaining the pH value at 8-9 to finally obtain the composition.

9. A composition according to claim 8, characterized in that: The molar ratio of folic acid, zinc ion and total glycosides of Centella asiatica is 1:0.5:0.5-1:1.5:

2.

10. A composition according to claim 8, characterized in that: The molar ratio of folic acid, zinc ion and total glycosides of Centella asiatica is 1:1:0.

46.

11. A composition according to claim 8, characterized in that: The alkaline solution includes one of an inorganic alkaline solution, an organic alkaline solution and an alkaline buffer solution.

12. A composition according to claim 11, characterized in that: The alkaline solution includes a series of buffer solutions of sodium hydroxide, potassium hydroxide, sodium carbonate, sodium bicarbonate, triethanolamine and tromethamine.

13. A composition according to claim 8, characterized in that: In the prepared composition, the mass content of folic acid is 0.01-10%.

14. A composition according to claim 13, characterized in that: In the prepared composition, the mass content of folic acid is 0.1-5%.

15. Use of the composition according to any one of claims 1 to 14, characterized in that: The composition is applied to cosmetics; the cosmetics have the effects of moisturizing, promoting the repair of damaged DNA, whitening, brightening the skin tone and resisting aging.

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