Preparation method and application of activator for nitrogen-fixing bacteria slow-release granules

Abstract

The application discloses a preparation method and application of an activator for nitrogen-removing and bacteria-fixing slow-release particles, and the activator contains 2.00-6.00% molasses, 2.0-8.0% sodium bicarbonate, 0.50-2.00% ferrous sulfate, 0.01-0.08% manganese sulfate, 0.05-0.10% magnesium sulfate, 0.01% vitamin C, 3.00-5.00% nitrifying bacteria (the nitrifying bacteria content is greater than 1 billion spores / ml), 3.00-5.00% denitrifying bacteria (the denitrifying bacteria content is greater than 1 billion spores / ml), 1.00-2.00% boric acid and 1.00-2.00% calcium chloride, and the activator is prepared by stirring with water. The activator can effectively restore the nitrogen-removing activity, significantly reduces the nitrogen-removing activity of the bacteria-fixing slow-release particles, and can maintain the nitrogen-removing effect for a long time. The activation treatment is convenient, low in cost and safe to the water body environment.

Description

Technical fields:

[0001] This invention relates to the preparation method and application of activators for nitrogen-fixing bacteria slow-release granules, belonging to the field of environmental protection technology. Background technology:

[0002] The enrichment of nitrogen in water bodies leads to algal blooms and reduced biodiversity.

[0003] The safest and most sustainable method for nitrogen removal from water bodies is biological nitrogen removal. Biological nitrogen removal is widely used in natural water bodies and wastewater treatment plants.

[0004] An economical and effective biological nitrogen removal method is the application of solid-release bacteria granules. These granules encapsulate nitrogen-removing bacteria or the nutrients required by the nitrogen-removing bacteria, as shown in our patent "Preparation Method and Application of Highly Efficient Nitrogen Removing Solid-Release Bacterial Granules" (Patent No.: ZL201911248165.X).

[0005] These slow-release granules are used in natural water bodies or sewage treatment plants. After being immersed in the water for a long time, their denitrification effect is significantly reduced, and their resistance to water erosion decreases, so they have to be discarded. Summary of the Invention:

[0006] The purpose of this invention is to provide a method for preparing an activator for nitrogen-removing and solidifying bacteria slow-release granules that is low in cost, easy to use, and highly efficient, as well as its application.

[0007] The nitrogen-removing and solidifying bacteria slow-release granule activator of the present invention contains molasses, sodium bicarbonate, ferrous sulfate, manganese sulfate, magnesium sulfate, vitamin C, nitrifying bacteria (nitrifying bacteria content > 1 billion spores / ml), denitrifying bacteria (denitrifying bacteria content > 1 billion spores / ml), boric acid, and calcium chloride. The contents (mass percentage) of the above ten components are as follows: 2.0-8.0%, 2.00-6.00%, 0.50-2.00%, 0.01-0.08%, 0.05-0.10%, 0.01%, 3.00-5.00%, 3.00-5.00%, 1.00-2.00%, and 1.00-2.00%, respectively. The activator is prepared by stirring with water to form the desired nitrogen-removing and solidifying bacteria slow-release granules.

[0008] Activation method: First, remove nitrogen-removing agents that have been used for a long time and whose denitrification effect has significantly decreased. Nitrogen fixing bacteria slow release granules air Dry, then soak in activator for 24 hours, then lift and dry. ​

[0009] The activator of this invention, in addition to supplementing the nitrifying and denitrifying bacteria and their nutrients required for nitrogen removal, also improves water resistance by solidifying the nitrogen-removing bacteria and nutrients in the slow-release granules through re-crosslinking.

[0010] The activator of the present application can effectively activate the nitrogen-removing activity of the nitrogen-removing bacteria sustained-release granule and prevent the growth of non-nitrogen-removing bacteria.

[0011] The nitrogen-removing bacteria sustained-release granule activated by the activator of the present application can continuously and effectively remove nitrogen pollution in water bodies.

[0012] The nitrogen-removing bacteria sustained-release granule activated by the activator of the present application is non-toxic to beneficial organisms (such as fish and daphnia) in water bodies. DETAILED DESCRIPTION

[0013] Test Example 1. Ammonia nitrogen removal effect of activated nitrogen-removing bacteria sustained-release granule in water bodies

[0014] Water sample: collected from the sewage treatment station of the North China Tree Garden in the east suburb of Tongzhou District, Beijing.

[0015] Experimental conditions: indoor, room temperature at 25-30°C.

[0016] Activation method: the nitrogen-removing bacteria sustained-release granule used for 4 months was taken back from the sewage treatment station of the North China Tree Garden in the east suburb of Tongzhou District, Beijing, and dried indoors for 3 days. Half of the granule was soaked in the activator solution of the present application for 1 day, and then dried for 3 days for standby; the other half was used as a control.

[0017] Treatment method: 20 g of activated nitrogen-removing bacteria sustained-release granule was added to 10 liters of sewage, and the same treatment was used as a control using non-activated nitrogen-removing bacteria sustained-release granule. Another blank sewage control was set up. Each treatment was repeated 4 times. After treatment, water samples were taken at 1 week and 4 weeks, respectively, and the ammonia nitrogen content was determined.

[0018] Ammonia nitrogen analysis method: salicylic acid spectrophotometry.

[0019] Experimental results: the experimental results are shown in Table 1. The experimental results show that the activator of the present application can significantly improve the ammonia nitrogen removal effect of the nitrogen-removing bacteria sustained-release granule and has a long lasting effect.

[0020] Table 1. Activation effect of the activator on the nitrogen-removing bacteria sustained-release granule

[0021]

[0022] Example 2: Safety evaluation of activated nitrogen-removing bacteria sustained-release granule on daphnia

[0023] The test fish was zebra fish, and the test daphnia was large daphnia.

[0024] Test method: the test refers to the test method of Daphnia magna toxicity in OECD 202, is carried out in a 100-mL beaker, and the exposure solution volume is 60 mL. Ten newly hatched juvenile daphnia with good activity and similar size within three days are gently transferred to each exposure solution, 0.1 g of the activated nitrogen-removing solid bacterial slow-release particles is placed in each beaker, three replicates are set for each treatment, and the beakers are placed in an illumination incubator for culture. After 24 h, the survival state of Daphnia magna is observed, and the mortality is investigated.

[0025] Experimental results: during the whole test period, the Daphnia magna in the treatment group and the control group moves normally, and no death phenomenon occurs. The experimental results show that the activated nitrogen-removing solid bacterial slow-release particles activated by the activator of the application is safe for daphnia in water.

Claims

1. The application of a nitrogen-removing, slow-release granular activator, characterized in that: The nitrogen-fixing bacteria slow-release granular activator contains molasses, sodium bicarbonate, ferrous sulfate, manganese sulfate, magnesium sulfate, vitamin C, nitrifying bacteria with a content >1 billion spores / ml, denitrifying bacteria with a content >1 billion spores / ml, boric acid, and calcium chloride. The mass percentage contents of the above ten components are 2.0-8.0%, 2.00-6.00%, 0.50-2.00%, 0.01-0.08%, and 0.05-0.10%, respectively. 0.01%, 3.00-5.00%, 3.00-5.00%, 1.00-2.00%, and 1.00-2.00% are mixed with water to form the required nitrogen-removing bacteria slow-release granule activator. The activator is used to activate nitrogen-removing bacteria slow-release granules for treating polluted water bodies. The activation method is as follows: First, dry the nitrogen-removing bacteria slow-release granules that have been used for a long time and whose nitrogen removal effect has significantly decreased. Then, soak them in the activator for 24 hours, and then take them out and dry them.

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