An anaerobic bacteria culture system

Abstract

The application relates to an anaerobic bacteria culture system, belonging to the technical field of anaerobic bacteria culture, which comprises an operation chamber, a transition chamber and a culture container, the operation chamber is communicated with the transition chamber, the culture container is used for culturing anaerobic bacteria, a sample feeding assembly and a sample moving assembly are arranged in the operation chamber, the sample moving assembly comprises a rotating disc and a driving piece for driving the rotating disc to rotate, the rotating disc is located in the operation chamber and is connected with the driving piece, a plurality of placing notches one are arranged on the peripheral wall of the rotating disc and are distributed at intervals along the peripheral wall of the rotating disc, the sample feeding assembly is arranged in the transition chamber, and the sample feeding assembly is used for moving the culture container from the transition chamber to the placing notches one of the rotating disc. The application has the effect that a staff member can conveniently take the culture container located at a deep position in the operation chamber.

Description

Technical Field

[0001] This application relates to the field of anaerobic bacteria culture, and in particular to an anaerobic bacteria culture system. Background Technology

[0002] Currently, anaerobic bacteria cultivation typically utilizes anaerobic jars or anaerobic culture chambers. Anaerobic culture chambers are generally used for culturing large quantities of anaerobic bacteria at once. An anaerobic culture chamber includes an interconnected operating chamber and a transition chamber, which is usually located to one side of the operating chamber. When anaerobic bacteria need to be cultured in an anaerobic culture chamber, the culture containers are placed in the transition chamber, which is then sealed. Since the operating chamber usually needs to be in an anaerobic or microaerobic environment, the culture containers are typically moved by using a glove with a concave opening on the operating chamber to remove them from the transition chamber and then move them to the operating chamber. Subsequent inoculation and cultivation of anaerobic bacteria are then performed on the culture containers.

[0003] However, since anaerobic bacteria incubators are typically used to culture a large number of anaerobic bacteria at once, they need to hold a large number of culture containers. This may result in some of the culture containers being located deep within the operating room. Consequently, long-arm gloves are often required for staff to reach these containers, which often necessitates extending the entire arm into the container. This results in staff being too close to the operating room when handling the containers, leading to a poor user experience. Furthermore, if staff do not have long enough arms, they may be unable to reach the containers or even manage to knock them over. Summary of the Invention

[0004] To enable staff to easily access culture containers located deep within the operating room from a normal distance, this application provides an anaerobic bacteria culture system.

[0005] The anaerobic bacteria culture system provided in this application adopts the following technical solution:

[0006] It includes an operating chamber, a transition chamber, and a culture container. The operating chamber and the transition chamber are connected. The culture container is used to culture anaerobic bacteria. The operating chamber is equipped with a sample delivery component and a sample transfer component. The sample transfer component includes a rotating disk and a drive component that drives the rotating disk to rotate. The rotating disk is located in the operating chamber and is connected to the drive component. Multiple placement slots are provided on the peripheral wall of the rotating disk. The multiple placement slots are distributed at intervals along the peripheral wall of the rotating disk. The sample delivery component is located in the transition chamber and is used to move the culture container from the transition chamber to the placement slot of the rotating disk.

[0007] By adopting the above technical solution, when it is necessary to move the culture container to the operating room, the culture container is first placed on the sample delivery component in the transition chamber. Then, the sample delivery component moves the culture container in the transition chamber to the placement slot 1 on the rotating disk in the operating room. This process is repeated until all placement slots 1 on the rotating disk are filled with culture containers. Subsequently, the rotating disk can be driven by a drive component to rotate at a fixed angle each time, so that each culture container on the rotating disk can be rotated to a position in the operating room that is convenient for the staff to operate. This allows the staff to pick up all the culture containers in the operating room by wearing gloves of normal length, without having to wear long gloves. It also reduces the probability of the culture containers being knocked over due to the staff's insufficient arm length when trying to pick them up.

[0008] Optionally, the drive unit is located below the operating chamber and installed on the outer wall of the operating chamber. An annular sealing groove is provided inside the operating chamber, and an annular sealing part is provided on the rotating disk. The annular sealing part and the annular sealing groove are connected by a plug-in fit. A sealing ring is provided in the annular sealing groove. When the annular sealing part and the sealing ring are connected by a plug-in fit, the annular sealing part and the sealing ring abut against each other.

[0009] By adopting the above technical solution, when the rotating disk is installed on the drive component, the annular sealing part enters into the annular sealing groove by insertion and abuts against the sealing ring in the annular sealing groove. This ensures that even if the drive component drives the rotating disk to rotate inside the operating room from outside the operating room, the gap between the drive component and the operating room will not easily cause gas exchange between the operating room and the outside of the operating room, thereby reducing the impact of external gas on the oxygen concentration inside the operating room.

[0010] Optionally, the sample delivery assembly includes a pusher, a pusher, and a placement seat. Both pushers are installed on the outer wall of the transition chamber. The placement seat is located inside the transition chamber and connected to pusher. The placement seat is used to place culture containers, and pusher is used to push the culture containers on the placement seat toward placement slot one.

[0011] By adopting the above technical solution, the sample delivery component includes a pusher one and a pusher two. The pusher one is equipped with a placement seat, which is used to place the culture container located in the transition chamber. Then, the pusher one pushes the placement seat so that the placement seat moves closer to the rotating disk to shorten the travel distance of the culture container. The pusher two pushes the culture container on the placement seat into the placement slot one on the rotating disk when the pusher one moves to the limit of its travel distance, thus realizing the movement of the culture container from the transition chamber to the operating chamber.

[0012] Optionally, the placement seat is provided with a second placement slot, the placement seat is provided with an insertion part, and the rotating disk is provided with an insertion groove. When the insertion part and the insertion groove are inserted and matched, a closed waist-shaped groove is formed between the first placement slot and the second placement slot, which allows the culture container to move.

[0013] By adopting the above technical solution, the placement seat is provided with a second placement slot and a plug-in part, while the rotating plate is provided with a plug-in groove. When the pushing member pushes the placement seat toward the rotating plate, the plug-in part on the placement seat and the plug-in groove on the rotating plate are plugged in and engaged, thereby forming a closed waist-shaped groove between the first placement slot and the second placement slot, which allows the culture container to move relatively smoothly when it moves into the first placement slot. At the same time, the waist-shaped groove formed between the first placement slot and the second placement slot guides the direction of movement of the culture container.

[0014] Optionally, a heating plate is provided in the transition chamber, which heats the culture container when it is placed on the placement seat in the transition chamber.

[0015] By adopting the above technical solution, a heating plate is provided in the transition chamber, and the heating plate is located below the second placement slot. This allows the culture container placed in the second placement slot to be heated before it is pushed out by the first pusher, so that the oxygen dissolved in the culture container can be initially discharged, reducing the oxygen concentration in the culture container, thereby improving the survival rate of anaerobic bacteria inoculated into the culture container.

[0016] Optionally, a gas replacement assembly is also provided in the operating chamber. The gas replacement assembly includes a lifting component and a sealing plate. The lifting component is installed above the operating chamber, and the sealing plate is located in the operating chamber and connected to the lifting component. Multiple sealing covers are provided on the side of the sealing plate away from the lifting component. The position of each sealing cover on the sealing plate corresponds to each placement slot. The sealing covers are adapted to the ports of the culture container. Each sealing cover is provided with a gas inlet and a gas outlet.

[0017] By adopting the above technical solution, when all the placement slots on the placement tray are filled with culture containers, the lifting component pushes the sealing plate down. When the lifting component descends to its limit position, the sealing cover on the sealing plate seals the culture containers, thus making the culture containers closed. Subsequently, mixed gas and nitrogen are introduced into the culture containers through the gas inlet to discharge the original gas in the culture containers from the gas outlet, thereby achieving gas replacement in the culture containers and creating an anaerobic environment inside the culture containers.

[0018] Optionally, a guide rod is also provided on the operating chamber. One end of the guide rod is located inside the operating chamber and is fixed perpendicularly to the sealing plate, while the other end of the guide rod is located outside the operating chamber. The guide rod is provided with an air inlet channel and an air outlet channel extending into the sealing plate. The gas inlet is connected to the air inlet channel, and the gas outlet is connected to the air outlet channel.

[0019] By adopting the above technical solution, since the gas inlet and gas outlet are connected to the air inlet and air outlet channels respectively, the gas inside the culture container can be replaced through the air inlet and air outlet channels on the guide rod, without the need to install gas pipes at the positions of each sealing cover on the sealing plate, thus improving the sealing performance of the operating chamber.

[0020] Optionally, the end of the air intake channel furthest from the gas inlet is the air intake end, and the end of the air outlet channel furthest from the gas outlet is the air outlet end. Both the air intake end and the air outlet end are located at the end of the guide rod furthest from the sealing disc. An air intake pipe is connected to the air intake end, and an air outlet pipe is connected to the air outlet end. Valves are installed on both the air intake pipe and the air outlet pipe.

[0021] By adopting the above technical solution, the air inlet pipe connected to the air inlet channel and the air outlet pipe connected to the air outlet channel will not easily interfere with the movement of the guide rod when the guide rod moves along its own axis. At the same time, valves are installed on both the air outlet pipe and the air inlet pipe to maintain the stability of the anaerobic environment inside the culture container and reduce the probability of gas exchange between the gas inside the culture container and the outside world through the air outlet pipe or the air inlet pipe.

[0022] In summary, this application includes at least the following beneficial technical effects:

[0023] 1. The culture containers in the transition chamber are moved to the rotating plate in the operating chamber by the sample delivery component. As the rotating plate rotates, the culture containers moved to the rotating plate are moved to a side that is convenient for the staff to operate, thereby making it easier for the staff to pick up the culture containers in the operating chamber.

[0024] 2. By setting the air intake end of the air intake channel and the air outlet end of the air outlet channel at the end of the guide rod away from the sealing plate, the guide rod will not easily interfere when it moves along the axis under the drive of the lifting component when the air intake channel and the air outlet channel are respectively connected to the air intake pipe and the air outlet pipe. Attached Figure Description

[0025] Figure 1 This is a three-dimensional schematic diagram of an anaerobic bacteria culture system according to this application;

[0026] Figure 2 yes Figure 1 A sectional view;

[0027] Figure 3 yes Figure 2 Enlarged view of point A in the middle;

[0028] Figure 4 yes Figure 1 A three-dimensional structural diagram of the sample delivery assembly, sample transfer assembly, and gas replacement assembly;

[0029] Explanation of reference numerals in the attached drawings: 1. Operating chamber; 11. Annular sealing groove; 12. Sealing ring; 2. Transition chamber; 21. Heating plate; 3. Culture container; 4. Sample delivery assembly; 41. Pushing component one; 42. Pushing component two; 43. Placement seat; 431. Placement slot two; 432. Insertion part; 5. Sample transfer assembly; 51. Rotating disk; 511. Annular sealing part; 512. Insertion slot; 52. Driving component; 53. Placement slot one; 6. Gas replacement assembly; 61. Lifting component; 62. Guide rod; 621. Air inlet channel; 6211. Air inlet end; 6212. Gas inlet; 622. Air outlet channel; 6221. Air outlet end; 6222. Gas outlet; 63. Sealing plate; 631. Sealing cover; 64. Air inlet pipe; 65. Air outlet pipe; 66. Valve. Detailed Implementation

[0030] The following is in conjunction with the appendix Figure 1-3 This application will be described in further detail.

[0031] This application discloses an anaerobic bacteria culture system, referring to... Figure 1 and Figure 2 The system includes an operating chamber 1 and a transition chamber 2 located on one side of the operating chamber 1. An operating panel is installed above the transition chamber 2. The operating panel is used to control the airflow and temperature in the operating chamber 1, reducing the probability of the nutrients in the culture container drying out and cracking due to excessive dryness in the operating chamber 1. The operating chamber 1 and the transition chamber 2 are connected. An acrylic glass is installed on one side of the operating chamber 1. Two gloves are recessed into the operating chamber 1 on the acrylic glass. This allows the staff to operate the relevant objects in the operating chamber 1 by putting their hands inside the gloves. At the same time, the acrylic glass also allows the staff to observe the situation inside the operating chamber 1 during operation.

[0032] Reference Figure 2 and Figure 3A sample transfer assembly 5 is provided in the operating chamber 1. The sample transfer assembly 5 includes a rotating disk 51 and a driving component 52. Multiple placement slots 53 extending toward the central axis of the rotating disk 51 are evenly arranged on the peripheral wall of the rotating disk 51. The placement slots 53 extend vertically and are used to place the culture container 3. An annular boss is provided on the outer wall of the culture container 3. The annular boss is made of a metal shell and a rubber liner. The rubber liner abuts against the outer wall of the culture container 3, and the metal shell is fitted on the rubber liner. When the culture container 3 is in the placement slot 53, the lower surface of the annular boss abuts against the upper surface of the rotating disk 51.

[0033] The drive component 52 is a stepper motor, located below the operating chamber 1 and connected to the outer wall of the operating chamber 1 by bolts. The rotating disk 51 is located inside the operating chamber 1. The output shaft of the drive component 52 passes through the outer wall of the operating chamber 1 and is fixedly connected to the rotating disk 51. This allows the rotating disk 51 to rotate along with the drive component 52, thereby enabling the culture containers 3 placed on the rotating disk 51 inside the operating chamber 1 to be rotated to a fixed angle and positioned closer to the plexiglass. This allows the staff to perform operations such as anaerobic inoculation on the culture containers 3 placed on the disk without having to get close to the operating chamber 1 to reach the culture containers 3 located further away. This allows the staff to remove the culture containers 3 from the rotating disk 51 with gloves of appropriate length. The entire process can be observed through the plexiglass, making it convenient for the staff to perform related operations on the culture containers 3 inside the operating chamber 1.

[0034] Furthermore, in order to prevent the culture container 3 from being thrown off the rotating disk 51 due to centrifugal force when the rotating disk 51 is rotating, a magnet is provided above the side wall of the placement slot 53 to attract the metal shell of the annular boss, thereby reducing the probability of the culture container 3 being thrown off when the rotating disk 51 is rotating.

[0035] An annular sealing groove 11 is provided inside the operating chamber 1, and an annular sealing part 511 is provided on the lower surface of the rotating disk 51. A sealing ring 12 is provided in the annular sealing groove 11. When the rotating disk 51 is connected to the output shaft of the drive component 52, the annular sealing part 511 on the lower surface of the rotating disk 51 is inserted into the annular sealing groove 11 by a plug-in fit and abuts against the sealing ring 12 in the annular sealing groove 11. This prevents the gas in the operating chamber 1 from easily exchanging gas with the outside through the gap between the output shaft of the drive component 52 and the outer wall of the operating chamber 1.

[0036] Inside the transition chamber 2, a sample delivery assembly 4 is installed. The sample delivery assembly 4 includes a first pusher 41, a second pusher 42, and a placement seat 43. Both the first pusher 41 and the second pusher 42 are cylinders, and the stroke of the first pusher 41 is shorter than that of the second pusher 42. A pusher seat is also provided at the movable end of the second pusher 42. Both the first pusher 41 and the second pusher 42 are installed on the side wall of the transition chamber 2 away from the operating chamber 1, and the second pusher 42 is located above the first pusher 41. The movable ends of the first pusher 41 and the second pusher 42 are both located inside the transition chamber 2. The placement seat 43 is connected to the movable end of the first pusher 41. The placement seat 43 is used to place the culture container 3 that enters the transition chamber 2. The pusher seat can push out the culture container 3 on the placement seat 43 under the push of the second pusher 42.

[0037] The placement seat 43 is provided with a second placement slot 431 for placing the culture container 3. The placement seat 43 is also provided with a plug-in part 432. On the rotating disk 51, each placement slot 53 has a plug-in groove 512 on both sides that is adapted to the plug-in part 432. This allows the plug-in part 432 on the placement seat 43 to connect with the plug-in groove 512 on the rotating disk 51 when the pusher 41 pushes the placement seat 43 toward the rotating disk 51. At this time, a closed waist-shaped groove is formed between the first placement slot 53 and the second placement slot 431. The upper surface of the rotating disk 51 is flush with the upper surface of the placement seat 43. This allows the culture container 3 in the second placement slot 431 to be pushed into the first placement slot 53 relatively smoothly. At the same time, the waist-shaped groove formed between the second placement slot 431 and the first placement slot 53 also guides the movement of the culture container 3.

[0038] Reference Figure 1 and Figure 2 Furthermore, a heating plate 21 is provided below the seat 43 in the transition chamber 2. This allows the bottom of the culture container 3 to be heated to a certain extent by the heating plate 21 when it is not pushed, so that the oxygen in the nutrients dissolved in the culture container 3 can be discharged from the culture container 3. As a result, when the culture container 3 enters the operating chamber 1 from the transition chamber 2, its internal oxygen content is lower, which is more suitable for inoculating anaerobic bacteria.

[0039] Reference Figure 2 and Figure 3To save time in creating a suitable environment for anaerobic bacteria within the operating chamber 1, a gas replacement assembly 6 is installed inside the operating chamber 1. The gas replacement assembly 6 includes a lifting component 61, a guide rod 62, and a sealing disc 63. The sealing disc 63 is located inside the operating chamber 1 and above the rotating disc 51. One end of the guide rod 62 is vertically connected to the upper surface of the sealing disc 63, while the other end is located outside the operating chamber 1. Two lifting components 61 are provided; each lifting component 61 is a cylinder. The lifting components 61 are symmetrically distributed on both sides of the guide rod 62 about its axis and are installed above the operating chamber 1. The movable end of the lifting component 61 is connected to the upper surface of the sealing disk 63, and multiple sealing covers 631 are provided on the lower surface of the sealing disk 63. The position of each sealing cover 631 corresponds to the position of each opening slot on the rotating disk 51. This allows the lifting component 61 to drive the sealing disk 63 to descend when a culture container 3 is placed on the rotating disk 51, so that the sealing cover 631 can cover the opening of the culture container 3, thereby forming a sealed space inside the culture container 3. This allows the culture container 3 to be anaerobic by simply replacing the gas inside it. Compared to replacing the gas in the entire operating chamber 1, this is faster and more efficient.

[0040] Reference Figure 2 and Figure 4 Inside the guide rod 62, there are independent air intake channels 621 and air outlet channels 622 extending into the sealing disc 63. The end of the air intake channel 621 on the guide rod 62 is the air intake end 6211, and the end of the air outlet channel 622 on the guide rod is the air outlet end 6221. Both the air intake end 6211 and the air outlet end 6221 are located at the end of the guide rod away from the sealing disc 63.

[0041] Each sealing cover 631 is provided with a gas inlet 6212 and a gas outlet 6222. The gas inlet 6212 is connected to the part of the air inlet channel 621 located inside the sealing plate 63, while the gas outlet 6222 is connected to the part of the air outlet channel 622 located inside the sealing plate 63.

[0042] This allows for the introduction of replacement gas into the inlet channel 621 when gas replacement is required in the culture container 3. The replacement gas enters the culture container 3 through the gas inlet 6212 on the inlet channel 621, and finally the gas in the culture container 3 enters the outlet channel 622 through the gas outlet 6222 on the outlet channel 622, and finally exits from the outlet channel 622, thereby removing the oxygen remaining in the culture container 3. Subsequently, the culture container 3 can be kept in an oxygen-free state by sealing the outlet channel 622.

[0043] An air inlet pipe 64 is provided at the air inlet end 6211 of the air inlet channel 621, and an air outlet pipe 65 is provided at the air outlet pipe 65. Valves 66 are provided on the air inlet pipe 64 and the air outlet pipe 65 respectively. The valves 66 are solenoid valves, which are opened or closed in an electrically controlled manner, so as to facilitate the operation of the staff. When the valves 66 on the air inlet pipe 64 and the air outlet pipe 65 are closed, the gas in the culture container 3 cannot increase or decrease, thereby stabilizing the anaerobic state in the culture container 3.

[0044] Since the air intake pipe 64 and air outlet pipe 65 connected to the guide rod 62 are both connected to the end of the guide rod 62 away from the sealing disc 63, the air intake pipe 64 and air outlet pipe 65 will not interfere with the movement of the guide rod 62 along its own axis.

[0045] A filter device can also be installed at the connection between the air intake pipe 64 and the air intake channel 621 to improve the purity of the input gas and reduce the possibility of contamination by miscellaneous bacteria.

[0046] Furthermore, an air inlet and an air outlet for replacing the gas inside the operating chamber 1 can also be provided on the outer wall of the operating chamber 1. By replacing the gas inside the operating chamber 1, the operating chamber 1 can be kept in a micro-oxygen state, while the culture container 3 is kept in an anaerobic state. This allows various anaerobic bacteria with different degrees of anaerobicity to be placed in the operating chamber 1 for cultivation, thereby increasing its applicability.

[0047] The implementation principle of this application embodiment is as follows: First, the culture container 3 containing nutrients is placed from the entrance of the transition chamber 2 into the placement slot 431 of the pusher seat. The heating plate 21 is activated for heating. Then, the pusher 41 is activated, which pushes the placement seat 43 so that the insertion part 432 on the placement seat 43 engages with the insertion slot 512 on the rotating disk 51. Then, the pusher 42 is activated, which pushes the culture container 3 on the placement seat 43 into the placement slot 53 on the rotating disk 51. This process is repeated until the rotating disk 51 contains the target number of culture containers 3. All culture containers 3 were inoculated with anaerobic bacteria. Then, the lifting device 61 was activated, which pushed the sealing plate 63 down, so that the multiple sealing caps 631 on the sealing plate 63 could accurately cover the opening of the culture container 3. When the sealing caps 631 were tightly closed on the culture container 3, replacement gas was delivered into the culture container 3 through the air inlet channel 621. It can also be connected to the air outlet pipe 65 through a vacuum pump and other related equipment to extract the gas that originally contained oxygen in the culture container 3. Afterwards, the valve 66 was closed, so that the culture container 3 was in an anaerobic state, thereby realizing the cultivation of anaerobic bacteria.

[0048] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. An anaerobic bacteria cultivation system, comprising an operating chamber (1), a transition chamber (2), and a cultivation container (3), wherein the operating chamber (1) and the transition chamber (2) are connected, and the cultivation container (3) is used for culturing anaerobic bacteria, characterized in that: The operating chamber (1) is equipped with a sample delivery assembly (4) and a sample transfer assembly (5). The sample transfer assembly (5) includes a rotating disk (51) and a driving component (52) for rotating the rotating disk (51). The rotating disk (51) is located in the operating chamber (1) and connected to the driving component (52). A plurality of placement slots (53) are provided on the peripheral wall of the rotating disk (51), and the plurality of placement slots (53) are distributed at intervals along the peripheral wall of the rotating disk (51). The sample delivery assembly (4) is located in the transition chamber (2). Used to move the culture container (3) from the transition chamber (2) to the placement slot (53) of the rotating disk (51); the driving member (52) is disposed below the operating chamber (1) and connected to the outer wall of the operating chamber (1); the operating chamber (1) is provided with a surrounding annular sealing groove (11); the rotating disk (51) is provided with an annular sealing part (511); the annular sealing groove (11) and the annular sealing part (511) both surround the connection between the driving member (52) and the rotating disk (511); the annular sealing part (511) 11) The annular sealing groove (11) is connected to the annular sealing groove (11) by a plug-in fit. A sealing ring (12) is provided in the annular sealing groove (11). The annular sealing part (511) abuts against the sealing ring (12). The sample delivery assembly (4) includes a first pusher (41), a second pusher (42), and a placement seat (43). The first pusher (41) and the second pusher (42) are both installed on the outer wall of the transition chamber (2). The placement seat (43) is located in the transition chamber (2) and connected to the first pusher (41). The first placement plate (43) is used to place culture containers. The second pusher (42) is used to push the culture containers on the placement plate (43) towards the first placement slot (53). The second placement slot (431) is provided on the placement plate (43). The second insertion part (432) is provided on the placement plate (43). The second insertion slot (51) is provided on the rotating plate (51). When the insertion part (432) and the insertion slot (512) are inserted and engaged, a closed waist-shaped groove that allows the culture containers to move is formed between the first placement slot (53) and the second placement slot (431).

2. The anaerobic bacteria cultivation system according to claim 1, characterized in that: A heating plate (21) is provided in the transition chamber (2). When the culture container is placed on the placement seat (43) in the transition chamber (2), the heating plate (21) heats the culture container.

3. The anaerobic bacteria cultivation system according to claim 1, characterized in that: The operating chamber (1) is also equipped with a gas replacement assembly (6). The gas replacement assembly (6) includes a lifting component (61) and a sealing plate (63). The lifting component (61) is installed above the operating chamber (1). The sealing plate (63) is located inside the operating chamber (1) and connected to the lifting component (61). The side of the sealing plate (63) away from the lifting component (61) is provided with multiple sealing covers (631). The position of each sealing cover (631) on the sealing plate (63) corresponds to each of the placement slots (53). The sealing cover (631) is adapted to the port of the culture container (3). Each sealing cover (631) is provided with a gas inlet (6212) and a gas outlet (6222).

4. The anaerobic bacteria cultivation system according to claim 3, characterized in that: A guide rod (62) is also provided on the operating chamber (1). One end of the guide rod (62) is located inside the operating chamber (1) and is fixed vertically to the sealing plate (63). The other end of the guide rod (62) is located outside the operating chamber (1). An air inlet channel (621) and an air outlet channel (622) extending into the sealing plate (63) are provided inside the guide rod (62). The gas inlet (6212) is connected to the air inlet channel (621), and the gas outlet (6222) is connected to the air outlet channel (622).

5. The anaerobic bacteria cultivation system according to claim 4, characterized in that: The end of the air intake channel (621) away from the gas inlet (6212) is the air intake end (6211), and the end of the air outlet channel (622) away from the gas outlet (6222) is the air outlet end (6221). The air intake end (6211) and the air outlet end (6221) are both located at the end of the guide rod (62) away from the sealing plate (63). An air intake pipe (64) is connected to the air intake end (6211), and an air outlet pipe (65) is connected to the air outlet end (6221). A valve (66) is provided on both the air intake pipe (64) and the air outlet pipe (65).

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