A method for constructing a composite nanochannel of a helical molecule and inorganic silicon nitride for single molecule sequencing

By embedding helical molecules into silicon nitride nanopores, a chiral nanochannel with high stability and long lifespan was constructed, solving the stability and controllability problems of nanopore technology and realizing efficient detection of single-molecule sequencing and chiral molecules.

CN116515974BActive Publication Date: 2026-06-19CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACAD OF SCI
Filing Date
2023-03-31
Publication Date
2026-06-19

AI Technical Summary

Technical Problem

In existing technologies, the low reproducibility of solid nanopores and the low stability of biological nanopores limit the large-scale application of nanopore technology. The stability and lifespan of nanochannels constructed on phospholipid membranes by helical molecules are limited by the phospholipid bilayer, and existing composite nanopore construction methods lack tunability.

Method used

By combining inorganic silicon nitride materials with organic helical chiral molecules, helical molecules are embedded in silicon nitride nanopores through electrophoretic force and electroosmotic force to form stable composite nanochannels. The bonding is achieved by electrostatic means and the embedding is strengthened by an applied bias voltage.

Benefits of technology

A chiral nanochannel with high stability, long lifetime, and high signal-to-noise ratio was constructed, which is suitable for single-molecule sequencing and ion-selective transport. It can regulate transport performance, improve detection time and spatial resolution, and is suitable for the identification of chiral molecules and the study of transport dynamics.

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Abstract

This invention provides a method for constructing helical molecules and inorganic silicon nitride composite nanochannels for single-molecule sequencing. After pretreating a silicon nitride nanopore chip to remove surface impurities and dielectric breakdown to form silicon nitride nanopores, the resulting silicon nitride nanopores are assembled in a liquid cell. A buffer solution containing positively charged or neutral helical molecules is added to one side of the flowcell, while a blank buffer solution is added to the other side. Then, under an applied bias voltage, the combined force of electrophoresis and electroosmosis drives the helical molecules to intercalate into the negatively charged silicon nitride nanopores. When the current on both sides of the flowcell suddenly drops significantly, a composite nanochannel is formed, with the helical molecules and silicon nitride channels electrostatically bonded. Increasing and continuously applying the voltage strengthens the embedded helical structure. This invention assembles helical molecules and silicon nitride nanochannels through in-situ voltage-driven assembly, resulting in stable, long-lived, and high signal-to-noise ratio chiral nanochannels.
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