Bactericidal composition containing glucan and isothiopyrad and application thereof

Abstract

The invention provides a fungicide composition containing glucan and isothianid, wherein the active ingredients thereof include glucan and isothianid, and the mass ratio of glucan to isothianid is 1:200-1:800. The fungicide composition has a significant synergistic effect and can be used for the prevention and treatment of agricultural diseases.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and particularly relates to a fungicide composition containing glucan and isothianidin and application thereof. Background Art

[0002] Cucumber is one of the main vegetables with the most extensive planting area in my country. Bacterial angular leaf spot of cucumber is an important disease of cucumber, caused by Pseudomonas syringae pv. lachrymans, which can affect both seedlings and adult plants. It mainly harms leaves, petioles, tendrils and fruits, and sometimes also infects stems and vines. When the cotyledons become diseased, they initially appear as water-soaked, nearly circular sunken spots, which then become slightly yellowish-brown and dry. Bacterial angular leaf spot of cucumber is common in Northeast China, North China and East China, especially in protected cucumbers in Northeast China, Inner Mongolia and other places, and in spring greenhouses in North China. When the damage is severe, the diseased leaf rate can be as high as about 70%, which is one of the important diseases of protected cucumbers. In addition to seed disinfection, disease-resistant varieties and enhanced cultivation management, chemical control is mainly used for the prevention and control of bacterial angular leaf spot of cucumber.

[0003] At present, patent technology CN201310013988.0 discloses a fungicide composition for plant bacterial and fungal diseases, characterized in that it contains zhongshengmycin and copper oxychloride, the weight ratio of zhongshengmycin and copper oxychloride is 1:15, and the fungicide composition prevents and treats cucumber bacterial angular spot. Patent technology CN201610466754.5 discloses a fungicide composition containing oxolinic acid and thiazole zinc, the active ingredients of which include oxolinic acid and thiazole zinc, characterized in that the weight ratio of oxolinic acid and thiazole zinc in the fungicide composition is 10:1-1:10, and the fungicide composition prevents and treats cucumber bacterial angular spot. Patent technology CN202210663906.6 discloses a composition agent for preventing and treating cucumber bacterial angular spot, the active ingredients of which are thiophanate-methyl and copper calcium sulfate, and the mass ratio of thiophanate-methyl to copper calcium sulfate is (1-10): (70-1). In summary, the current control agents for cucumber bacterial angular leaf spot all act directly on the pathogen. Bacterial diseases are very likely to develop resistance to direct-acting agents during the control process, resulting in low control effects on bacterial diseases. Therefore, it is urgent to develop new agents that can delay resistance and effectively control cucumber bacterial angular leaf spot. Summary of the invention

[0004] In order to solve the above technical problems, the present invention provides a fungicide composition containing glucan and isotianil, wherein the fungicide composition has high preventive effect on cucumber bacterial angular spot disease and delays resistance.

[0005] To achieve the above object, the present invention adopts the following technical solutions:

[0006] The bactericidal composition containing glucan and isotianil provided by the invention consists of the following effective ingredients, adjuvants and carriers.

[0007] The active ingredient in the bactericidal composition is compounded with glucan and isothianil, the optimal mass ratio of glucan to isothianil is 1:200-1:800, and preferably the optimal mass ratio of glucan to isothianil is 1:400.

[0008] The auxiliary agents in the bactericidal composition include tea saponin and polyhydroxyalkanoate, and the optimal content of tea saponin and polyhydroxyalkanoate is: tea saponin accounts for 4.0-16.0% of the total mass of the bactericidal composition, and polyhydroxyalkanoate accounts for 0.2-0.8% of the total mass of the bactericidal composition. Preferably, tea saponin accounts for 12.0% of the total mass of the bactericidal composition, and polyhydroxyalkanoate accounts for 0.6% of the total mass of the bactericidal composition.

[0009] The carriers in the bactericidal composition are white carbon black and kaolin.

[0010] The preparation steps of the bactericidal composition are as follows: weighing various raw materials in proportion, mixing them evenly in a mixing cylinder, crushing them into particles with a jet mill to a size of 1 to 5 microns, and then mixing them evenly to prepare the composition of the present invention.

[0011] The bactericidal composition is used to prevent and treat agricultural diseases, and preferably the agricultural disease is cucumber bacterial angular leaf spot.

[0012] Compared with the prior art, the present invention has the following beneficial effects:

[0013] (1) The bactericidal composition of the present invention has a synergistic effect on bacterial angular leaf spot of cucumber. When the mass ratio of glucan and isothiopyrad is 1:200-1:800, a synergistic effect is shown, especially when the mass ratio is 1:400, the synergistic effect is significant.

[0014] (2) Both glucan and isotianil in the fungicide composition of the present invention are inducers, which do not directly act on pathogens, but induce plants to produce disease resistance. Therefore, the composite composition of glucan and isotianil can effectively delay bacterial resistance.

[0015] (3) The adjuvant of the present invention is a composite adjuvant, which is a mixture of tea saponin and polyhydroxyalkanoate. Tea saponin acts as a wetting and dispersing agent, reduces surface tension, and improves the adhesion of the pesticide solution. Polyhydroxyalkanoate can increase the viscosity of the liquid medicine and improve the adhesion of the liquid medicine. Tea saponin and polyhydroxyalkanoate are scientifically compounded and used, and the synergistic effect improves the absorption and utilization of the active ingredients by crops, thereby improving the control effect of the composition on cucumber bacterial angular spot disease. DETAILED DESCRIPTION

[0016] The present invention will be further described below in conjunction with examples, but the present invention is not limited by the following examples.

[0017] Example 1: Indoor potted plant activity test

[0018] Experimental crops: cucumber.

[0019] Test pathogen: Cucumber bacterial angular leaf spot pathogen was isolated from diseased leaves of vegetable greenhouse in Jingyang County, Shaanxi Province, and purified on plates. The purified strain was inoculated on test tube slant culture medium, cultured at 28°C for 48 hours, and then stored in a refrigerator for later use.

[0020] Test agents: 95% polyglucan technical (produced by Shandong Kailinong Biotechnology Co., Ltd.), 96% isothiopyrad technical (produced by Bayer AG) and a combination of the two technicals added in a mass ratio.

[0021] Test steps:

[0022] (1) Preparation of bacterial suspension: Activate the cucumber bacterial angular leaf spot bacteria on a NA / LB solid plate. After culturing in a 28°C incubator, pick a single colony and transfer it to a NB / LB liquid medium. Incubate it on a shaker at 28°C and 160 rpm for 36 hours and prepare a bacterial suspension for later use.

[0023] (2) Preparation of the drug: Dissolve the original drug of dextran with water, dissolve the original drug of isothiopyrad with ethanol, and dilute them with 0.1% Tween-80 aqueous solution into 5 concentration gradients for determining the single-dose EC 50 Glucan and isothiopyrad were prepared into different pharmaceutical compositions according to different mass ratios of active ingredients, and diluted into 5 series of concentrations according to the above method to determine the EC of different pharmaceutical compositions. 50 .

[0024] (3) Chemical treatment: Select potted cucumbers with the same growth and spray the above chemicals evenly on the cucumber plants. Repeat 3 times for each treatment, with 5 pots for each repeat, and set a treatment without chemical as a blank control.

[0025] (4) Inoculation and culture: Two days after the treatment, the cucumber plants were sprayed with a suspension of cucumber bacterial angular leaf spot pathogen, including the control without the agent. After inoculation, the cucumber seedlings were placed in a moisturizing cabinet with a relative humidity of 90% and a temperature of 26-28°C for 24 hours, and then transferred to a normal greenhouse for culture.

[0026] (5) Disease investigation: After inoculation, wait until the control without pesticide is fully diseased, and calculate the disease index and control effect. Cucumber bacterial angular spot lesions are graded according to the leaf surface area occupied. Grade 0: no lesions; Grade 1: 0%-5%; Grade 3: 5%-25%; Grade 5: 25%-50%; Grade 7: 50%-75%; Grade 9: more than 75%.

[0027] (6) Data Statistical Analysis

[0028] According to the survey data, the disease index and control effect of each treatment were calculated.

[0029] Disease index = 100 × ∑ (number of diseased leaves at each level × relative level value) / (total number of leaves surveyed × 9)

[0030] Control effect (%) = 100 × (control disease index - treatment disease index) / control disease index.

[0031] The EC of each agent was calculated by regression analysis based on the logarithmic value of each agent concentration and the corresponding control effect probability value. 50 The interaction of the mixture was evaluated according to the Wadley method, where SR = 0.5-1.5, the two drugs have an additive effect; SR ≤ 0.5, it is an antagonistic effect; SR ≥ 1.5, it is a synergistic effect.

[0032] EC 50 (theoretical value) = (a+b) / (a / EC 50a +b / EC 50b )

[0033] SR=EC 50 (Theoretical value) / EC 50 (Actual value)

[0034] The results of the indoor potted plant activity test are shown in Table 1.

[0035] Table 1 Results of the indoor potted plant activity test of the fungicidal composition against bacterial angular leaf spot of cucumber

[0036]

[0037] It can be seen from Table 1 that the synergistic coefficients of glucan and isothianid in the mass ratio range of 1:200-1:800 are all greater than 1.5, indicating that the combination of glucan and isothianid has a synergistic effect on cucumber bacterial angular leaf spot, especially when the mass ratio is 1:400, the synergistic coefficient is 2.53, which is significant.

[0038] Example 2: Composition Example

[0039] Composition Example 1

[0040]

[0041] The raw materials are weighed according to the above proportions, mixed evenly in a mixing tank, crushed to a particle size of 1 to 5 μm by a jet mill, and then mixed evenly to obtain Composition Example 1.

[0042] Composition Example 3.

[0043] Composition Example 2

[0044]

[0045] The raw materials are weighed according to the above proportions, mixed evenly in a mixing tank, crushed to a particle size of 1 to 5 microns by a jet mill, and then mixed evenly to obtain composition Example 2.

[0046] Composition Example 3

[0047]

[0048] The raw materials are weighed according to the above proportions, mixed evenly in a mixing tank, crushed to a particle size of 1 to 5 μm by a jet mill, and then mixed evenly to obtain composition Example 3.

[0049] Example 3: Field efficacy example

[0050] The field efficacy test was carried out at a greenhouse vegetable base in Jingyang County, Shaanxi Province. The spray was applied evenly at the early stage of bacterial angular leaf spot disease of cucumber. A control of pure water and a control of the pesticide with conventional adjuvants added were set up.

[0051] Disease investigation: After the clear water control is fully diseased, calculate the disease index and control effect. Cucumber bacterial angular spot lesions are graded according to the leaf surface area occupied. Grade 0: no lesions; Grade 1: 0%-5%; Grade 3: 5%-25%; Grade 5: 25%-50%; Grade 7: 50%-75%; Grade 9: more than 75%.

[0052] (6) Data Statistical Analysis

[0053] According to the survey data, the disease index and control effect of each treatment were calculated.

[0054] Disease index = 100 × ∑ (number of diseased leaves at each level × relative level value) / (total number of leaves surveyed × 9)

[0055] Control effect (%) = 100 × (control disease index - treatment disease index) / control disease index.

[0056] The results of the field efficacy test are shown in Table 2.

[0057] Table 2 Field efficacy test results of fungicidal composition against cucumber bacterial angular leaf spot pathogen

[0058]

[0059] As can be seen from Table 2, the composite of glucan and isothiopyrad in a certain proportion has a significantly better control effect on cucumber bacterial angular spot disease than a single agent, and the dosage is significantly reduced compared with a single agent; adding compound adjuvants such as tea saponin and polyhydroxyalkanoate to the composition significantly improves its control effect than adding conventional adjuvants, and the maximum control effect is as high as 92.5%.

[0060] The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present invention and various different options and changes. The scope of the present invention is intended to be limited by the claims and their equivalents.

Claims

1. A fungicidal composition containing glucan and isothiopyrad, characterized in that: The active ingredients of the bactericidal composition are composed of glucan and isotianil, and the mass ratio of glucan to isotianil is 1:200-1:

800.

2. The bactericidal composition according to claim 1, characterized in that: The mass ratio of dextran to isothiopyrad is 1:

400.

3. The bactericidal composition according to claim 1, characterized in that: The composition consists of effective ingredients and auxiliary agents.

4. The bactericidal composition according to claim 3, characterized in that: The auxiliary agent comprises tea saponin and polyhydroxyalkanoate, and the auxiliary agent also comprises carrier white carbon black and kaolin. The tea saponin accounts for 4.0-16.0% of the total mass of the sterilization composition, and the polyhydroxyalkanoate accounts for 0.2-0.8% of the total mass of the sterilization composition.

5. The bactericidal composition according to claim 4, characterized in that: The tea saponin accounts for 12.0% of the total mass of the bactericidal composition, and the polyhydroxyalkanoate accounts for 0.6% of the total mass of the bactericidal composition.

6. Use of the bactericidal composition according to any one of claims 1 to 5 in preventing and controlling agricultural diseases, characterized in that: The agricultural disease is cucumber bacterial angular leaf spot.

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