Bactericidal composition containing metconazole and prochloraz and application thereof

Abstract

The invention relates to the field of wheat disease control pesticides, in particular to a bactericidal composition containing metconazole and prochloraz and application. The invention discloses a bactericidal composition containing metconazole and prochloraz. The bactericidal composition is prepared from the following components in parts by weight: 10 parts of metconazole, 10-40 parts of prochloraz, 2-10 parts of a dispersing agent and 1-5 parts of a wetting agent, the dispersing agent comprises at least one of fatty alcohol polyoxyethylene ether sulfate, naphthaldehyde condensate sulfonate and polyacrylate; the wetting agent comprises at least one of fatty alcohol-polyoxyethylene ether sulfate, lauryl sodium sulfate and fatty alcohol-polyoxyethylene ether. The metconazole and the prochloraz are compounded, so that the prevention and treatment effects on wheat scab, wheat rust and wheat powdery mildew can be improved, and the drug resistance of pathogenic bacteria can be well delayed. In addition, through mutual cooperation of the dispersing agent and the wetting agent, the utilization rate and the control effect of the pesticide are improved, and loss and waste of the pesticide are reduced.

Description

Technical Field

[0001] The present application relates to the field of pesticides for controlling wheat diseases, and in particular to a fungicide composition containing metconazole and prochloraz and its application. Background Art

[0002] Wheat fusarium head blight, also known as ear rot, is a disease of wheat caused by infection of various Fusarium fungi. It can occur from the seedling stage to the ear stage, causing seedling rot, stem base rot, stalk rot and ear rot, with ear rot being the most harmful. When the humidity is high, pink mold layer can be seen on the diseased parts. After wheat is infected, the thousand-grain weight decreases, the germination rate decreases, the germination potential weakens, the flour yield is low, the flour quality is poor, the color is dark, and the commercial value decreases. Diseased wheat contains toxins such as vomiting toxins and estrogen-like toxins, which can cause acute poisoning after being eaten by humans and animals. Diseased wheat grains contain toxins such as deoxynivalenol and zearalenone, which can also cause pregnant female animals to miscarry after poisoning. Wheat fusarium head blight often spreads in winter wheat areas in southern China, such as the winter wheat areas in the middle and lower reaches of the Yangtze River, the winter wheat areas in Sichuan and Yunnan, and the winter wheat areas in southern China. It can also spread and cause disasters in the spring wheat areas of the Sanjiang Plain in the northeast in rainy years. It is particularly serious in the rainy and humid temperate zone. In general, the diseased ear rate in an epidemic year reaches 50-100%, with a yield loss of 10-20%. In a moderate year, the diseased ear rate is 30-50%, with a yield loss of 5-10%.

[0003] Wheat rust is also called jaundice. There are three main types of wheat rust: stem rust, leaf rust and stripe rust. They are caused by stem rust, leaf rust and stripe rust respectively. They mainly harm wheat leaves, but can also harm leaf sheaths, stems and ears. In mild cases, wheat grains are not full, and in severe cases, wheat plants die and cannot produce ears. The main characteristics of wheat rust are strong harm and wide range of influence. Once infected, it will have a serious impact on wheat growth, thereby reducing wheat yields. Wheat rust is a fungal disease. The most frequent wheat rusts are stem rust, leaf rust, and stripe rust. Among them, leaf rust has the highest range, incidence, and harmfulness among the three diseases. Generally, the yield reduction is 5-15%, and in severe cases, it can reach more than 50%.

[0004] Wheat powdery mildew is a disease of wheat caused by Blumeria graminaceus of the Poaceae family. It mainly harms leaves, and in severe cases, leaf sheaths, stems, and ears are all infected. In the early stage of the disease, small yellow spots can be seen on the diseased part. As the disease worsens, the disease spots gradually develop into lesions, which are oval or round in shape, with a layer of powdery mold on the surface. In the middle stage, they are white-gray, and in the later stage, they are light brown, and closed thecae are produced. When the disease is mild, the mildew spots are scattered. As the disease worsens, the mildew spots gradually expand into pieces and eventually cover the entire leaf. The lower part of the lesions and the surrounding tissues turn green, and the diseased leaves turn yellow and wither early. If the disease affects the stems and leaf sheaths, the whole plant will fall over. The plants are short, thin, and weak, with small ears and few grains, and the thousand-grain weight is significantly reduced, which ultimately affects wheat yields.

[0005] At present, there are many fungal disease control agents for wheat scab, wheat rust and wheat powdery mildew, such as tebuconazole, methyl thiophanate, carbendazim, thiophanate-methyl, pyraclostrobin, hexaconazole, etc. However, since these drugs were launched earlier and wheat pathogens will produce obvious resistance after continuous use, the expected control effect cannot be achieved. Therefore, it is necessary to provide a new agent for controlling wheat fungal diseases. Summary of the invention

[0006] In order to provide a new agent for preventing and treating fungal diseases of wheat, the present application provides a fungicide composition containing metconazole and prochloraz. The compound of metconazole and prochloraz has a good control effect on wheat ergot, wheat rust and wheat powdery mildew, and the action mechanisms of metconazole and prochloraz on pathogens are different, which also effectively delays the development of drug resistance of pathogens.

[0007] In the first aspect, the present application provides a fungicide composition containing metconazole and prochloraz, which adopts the following technical solution: A fungicide composition containing metconazole and prochloraz, comprising the following components in parts by weight: 10 parts of metconazole, 10-40 parts of prochloraz, 2-10 parts of a dispersant, and 1-5 parts of a wetting agent; The dispersant includes at least one of fatty alcohol polyoxyethylene ether sulfate, naphthaldehyde condensate sulfonate, and polyacrylate; the wetting agent includes at least one of fatty alcohol polyoxyethylene ether sulfate, sodium lauryl sulfate, and fatty alcohol polyoxyethylene ether.

[0008] By adopting the above technical scheme, metconazole mainly destroys the cell membrane of pathogens by inhibiting the biosynthesis of ergosterol, thereby inhibiting the growth and reproduction of pathogens, especially having a good control effect on wheat fusarium head blight. Prochloraz mainly works by inhibiting the key enzymes in the ergosterol biosynthesis pathway of pathogens. It has a significant control effect on wheat rust and wheat powdery mildew, and also helps to enhance the stress resistance of crops.

[0009] After the two fungicides are compounded, due to their different mechanisms of action, they can produce a synergistic effect, making the overall control effect better than the use of a single agent. The compound agent can more comprehensively inhibit the growth and reproduction of pathogens and reduce the damage of pathogens to crops. The long-term use of a single agent can easily lead to drug resistance in pathogens, while the use of compound agents can delay the development of resistance. Because pathogens need to face two fungicides with different mechanisms of action at the same time, it is more difficult for them to develop resistance.

[0010] Dispersants can significantly reduce the surface tension of pesticide particles in water, allowing pesticide particles to be more evenly dispersed in the solvent to form a stable suspension. Dispersants such as fatty alcohol polyoxyethylene ether sulfate, naphthaldehyde condensate sulfonate, and polyacrylate have their own characteristics and can provide effective dispersion effects for different types of pesticide particles and solvent systems. Their combined use can further enhance the dispersibility of pesticides and make it easier for pesticides to spread on the surface of crops.

[0011] Wetting agents can reduce the contact angle of pesticide solution on the crop surface, making it easier for pesticides to wet the crop surface and form a uniform liquid film. This helps to improve the permeability and adhesion of pesticides, allowing them to be more fully absorbed and utilized by crops. Wetting agents such as fatty alcohol polyoxyethylene ether sulfate, sodium lauryl sulfate, and fatty alcohol polyoxyethylene ether have excellent wetting properties in their respective fields. Their combined use can further enhance the wetting effect of pesticides on the crop surface, ensuring that the pesticides can be evenly and effectively covered on the crop surface.

[0012] The cooperation between dispersants and wetting agents can form a synergistic effect, making the pesticide particles dispersed more evenly in the solvent and forming a more stable liquid film on the surface of the crop. This synergistic effect helps to improve the utilization rate and control effect of pesticides and reduce the loss and waste of pesticides.

[0013] In the present application, by compounding metconazole and prochloraz, the control effect on wheat scab, wheat rust and wheat powdery mildew can be improved, and at the same time, due to the different mechanisms of action of the two, the drug resistance of pathogens can be well delayed. In addition, by the mutual coordination of dispersants such as fatty alcohol polyoxyethylene ether sulfate and wetting agents such as sodium lauryl sulfate, the pesticide particles are dispersed more evenly in the solvent, and a more stable liquid film is formed on the crop surface. This synergistic effect helps to improve the utilization rate and control effect of pesticides and reduce the loss and waste of pesticides.

[0014] Preferably, the mass ratio of the metconazole to the prochloraz is 1:2-3.

[0015] By adopting the above technical scheme, prochloraz has a significant control effect on wheat rust and wheat powdery mildew. When the prochloraz content is too low, it may be difficult to effectively control these two diseases, resulting in poor control effect; in addition, too low prochloraz content will also weaken the synergistic effect with metconazole; metconazole and prochloraz are the main fungicidal ingredients in the fungicide composition. In order to reduce the risk of phytotoxicity, the sum of the contents of metconazole and prochloraz in the fungicide composition has a certain range. When the prochloraz content is too high, it will inevitably lead to a relative decrease in the metconazole content, which is not conducive to the synergistic effect of the two; in addition, if the prochloraz content is too high, there may also be a risk of phytotoxicity; for this reason, the applicant finally determined after a lot of research and experimental verification that the mass ratio of metconazole to prochloraz in this application is preferably as mentioned above.

[0016] Preferably, the mass ratio of the prochloraz to the fatty alcohol polyoxyethylene ether sulfate is 25:8-12.

[0017] By adopting the above technical solution, fatty alcohol polyoxyethylene ether sulfate (AES) is a nonionic-anionic surfactant. Due to its unique molecular structure and properties, it can play a role in pesticide formulations as both a dispersant and a wetting agent.

[0018] In the molecular structure of AES, long-chain fatty alcohols provide good hydrophobicity and affinity with pesticide particles, while polyoxyethylene ether chains provide hydrophilicity and solubility in water. This amphiphilicity enables AES to effectively adsorb on the surface of pesticide particles, reduce the surface tension between particles, thereby preventing particle aggregation and improving the dispersibility and suspension stability of pesticides in water. The anionic sulfate part of AES gives it good wetting properties. When the pesticide solution contacts the crop surface, AES can quickly reduce the interfacial tension between the solution and the crop surface, making it easier for the pesticide solution to spread and penetrate on the crop surface.

[0019] If the AES content is too low, its dispersing and wetting effects will be significantly weakened. Insufficient dispersants will cause pesticide particles to easily aggregate and settle in water, affecting the suspension stability and spraying uniformity of the pesticide; insufficient wetting agents will make it difficult for the pesticide solution to spread and penetrate on the crop surface, reducing the utilization rate and control effect of the pesticide.

[0020] Although increasing the AES content may improve the dispersion and wetting effects to a certain extent, too high a content is not always beneficial. On the one hand, too much AES may increase the cost of pesticide formulations; on the other hand, too high a surfactant concentration may cause problems such as excessive foaming and decreased stability. In addition, as a surfactant, AES may form micelles or aggregates after reaching a certain concentration, which in turn affects its adsorption and dispersion effects on the surface of pesticide particles.

[0021] Preferably, the mass ratio of the prochloraz to the naphthaldehyde condensate sulfonate is 25:3-6.

[0022] By adopting the above technical solution, the naphthaldehyde condensate sulfonate can effectively disperse the prochloraz particles in the solvent or suspension to prevent them from agglomerating or precipitating. If the content is too low, it may not provide sufficient dispersing force, resulting in the aggregation of prochloraz particles, affecting the uniformity and stability of the preparation.

[0023] If the content of naphthaldehyde condensate sulfonate is too high, although the dispersibility may be further improved, it may also cause the system to be too viscous, increase the fluidity resistance of the preparation, and may even affect the spraying effect of the preparation.

[0024] Preferably, the mass ratio of prochloraz to sodium lauryl sulfate is 25:2-4.

[0025] By adopting the above technical solution, sodium dodecyl sulfate is used as a wetting agent, and its main function is to help the preparation to better wet the target surface (such as wheat leaves), thereby improving the coverage and adhesion of prochloraz. If the content is too low, the wetting ability may be insufficient, resulting in the inability of the preparation to form a uniform covering layer on the target surface, affecting the absorption and efficacy of prochloraz.

[0026] If the content of sodium dodecyl sulfate is too high, although it will further enhance the wetting ability, it may also cause the preparation to form too much wetting layer on the target surface, or even cause flowing or dripping. This not only wastes the agent, but may also cause a large amount of agent to accumulate on the bottom leaves, which can easily lead to drug damage.

[0027] The right amount of sodium lauryl sulfate helps maintain the physical stability of the formulation, such as preventing particle agglomeration and stratification. Too low a content may not provide sufficient stability, while too high a content may introduce additional variables and affect the overall stability of the formulation.

[0028] Preferably, the mass ratio of prochloraz to fatty alcohol polyoxyethylene ether is 25:1.5-3.5.

[0029] By adopting the above technical solution, the fatty alcohol polyoxyethylene ether is used as a wetting agent, and its main function is to help the preparation to better wet the target surface (such as wheat leaves), thereby improving the coverage and adhesion of prochloraz. If the content is too low, the wetting ability may be insufficient, resulting in the inability of the preparation to form a uniform covering layer on the target surface, affecting the absorption of prochloraz and the efficacy of prochloraz.

[0030] Fatty alcohol polyoxyethylene ether can also be used as an emulsifier. If the content of fatty alcohol polyoxyethylene ether is too high, the fungicide composition will become an emulsifier, making it difficult to prepare a suspension and a water-dispersible granule.

[0031] Preferably, the fungicidal composition containing metconazole and prochloraz further comprises nikkomycin Z.

[0032] By adopting the above technical solution, nikkomycin Z, as an inhibitor targeting UDP-N-acetylglucosamine synthetase, can effectively block the synthesis pathway of chitin. Chitin is an important component of the cell wall of pathogens. Its absence or blocked synthesis will cause the cell wall structure to become fragile, thereby affecting the integrity and stability of the cell wall. This not only weakens the physical defense ability of pathogens, but also makes the cytoplasm in the cell more susceptible to external factors.

[0033] Metconazole and prochloraz mainly act on the cell membrane of pathogens, especially by affecting the synthesis of ergosterol to interfere with the formation and function of the cell membrane. When the synthesis of ergosterol is blocked, the integrity of the cell membrane will be damaged, resulting in increased permeability of the cell membrane, and ultimately causing the leakage of substances in the cell and cell death.

[0034] Nikkomycin Z destroys the cell wall, making the external defense line of the pathogen vulnerable; while metconazole and prochloraz affect the cell membrane and disintegrate the pathogen from the inside. This internal and external attack method causes the pathogen to be hit at multiple levels, greatly improving the bactericidal effect. The blockage of chitin synthesis caused by nikkomycin Z will cause changes in the cytoplasmic osmotic pressure of the pathogen. This osmotic pressure imbalance will make the cell more susceptible to external pressure, thereby accelerating cell rupture and death. At the same time, because the cell membrane has been affected by metconazole and prochloraz, its ability to resist external pressure is weakened, so this change in osmotic pressure will further aggravate cell damage. The different mechanisms of action of the three make it difficult for pathogens to develop resistance to these three fungicides at the same time. This diversified mode of action helps to extend the service life of fungicides and improve the prevention and control effect.

[0035] In a second aspect, the present application provides an application of a fungicide composition containing metconazole and prochloraz, using the following technical solution: A fungicide composition containing metconazole and prochloraz is used in the prevention and treatment of wheat scab, wheat rust and wheat powdery mildew.

[0036] By adopting the above technical scheme, metconazole mainly destroys the cell membrane of pathogens by inhibiting the biosynthesis of ergosterol, thereby inhibiting the growth and reproduction of pathogens, especially having a good control effect on wheat fusarium head blight. Prochloraz mainly works by inhibiting the key enzymes in the ergosterol biosynthesis pathway of pathogens. It has a significant control effect on wheat rust and wheat powdery mildew, and also helps to enhance the stress resistance of crops. Prochloraz also has a systemic conduction effect, which can be absorbed by wheat and conducted to various parts to comprehensively prevent and control diseases.

[0037] Preferably, the fungicidal composition containing metconazole and prochloraz is formulated to include at least one of a suspension concentrate, an emulsifiable concentrate and a water dispersible granule.

[0038] By adopting the above technical scheme, metconazole and prochloraz act together on pathogens through different mechanisms of action, which can significantly enhance the bactericidal effect and improve the control efficiency. The existence of multiple dosage forms such as suspension concentrate, emulsifiable concentrate and water-dispersible granules allows farmers to choose the appropriate dosage form according to actual usage and needs. For example, suspension concentrate has good suspension and dispersibility and is suitable for spraying; emulsifiable concentrate has high permeability and systemicity and is suitable for the prevention and control of diseases that require rapid absorption and conduction; water-dispersible granules are easy to store and transport, and only need to be simply dissolved when used. These dosage forms usually have good stability and solubility, are easy to operate and control during use, and reduce the difficulty of use for farmers.

[0039] Preferably, when the fungicide composition containing metconazole and prochloraz is formulated into an emulsifiable concentrate, an emulsifier needs to be added, and the emulsifier includes fatty alcohol polyoxyethylene ether.

[0040] By adopting the above technical scheme, the emulsifier includes fatty alcohol polyoxyethylene ether, castor oil polyoxyethylene ether, sorbitan fatty acid ester, polyethylene glycol oleate, etc., among which the fatty alcohol polyoxyethylene ether can be used as both a wetting agent and an emulsifier.

[0041] Fatty alcohol polyoxyethylene ether is a nonionic surfactant prepared by the addition reaction of fatty alcohol and ethylene oxide. Its molecular structure contains both lipophilic fatty alcohol groups and hydrophilic polyoxyethylene ether segments. This amphiphilic structure enables fatty alcohol polyoxyethylene ether to form a stable adsorption layer at the oil-water interface, thereby reducing the interfacial tension between oil and water, making it easier for the oil phase and the water phase to form a stable emulsion. During the emulsification process, the molecules of fatty alcohol polyoxyethylene ether will form a protective film on the surface of the oil droplets to prevent the aggregation of the oil droplets, thereby maintaining the stability of the emulsion. Some fatty alcohol polyoxyethylene ethers also have a certain solubilizing effect, which can solubilize some oil-soluble or water-soluble active ingredients in the emulsion, thereby improving the uniformity and stability of the preparation.

[0042] As a wetting agent, fatty alcohol polyoxyethylene ether can significantly reduce the surface tension of water, making it easier for water to penetrate into the tiny gaps on solid surfaces (such as plant leaves or pathogen surfaces), thereby achieving a better wetting effect. This wetting effect helps the active ingredients in the fungicide composition to be more evenly distributed on the target surface, improving the efficacy.

[0043] In summary, this application has the following beneficial effects: 1. Since the compound of metconazole and prochloraz in this application can improve the control effect of wheat scab, wheat rust and wheat powdery mildew, and at the same time, due to the different action mechanisms of the two, the drug resistance of pathogens can be well delayed; in addition, through the mutual cooperation of dispersants such as fatty alcohol polyoxyethylene ether sulfate and wetting agents such as sodium dodecyl sulfate, the pesticide particles are dispersed more evenly in the solvent, and a more stable liquid film is formed on the surface of the crop. This synergistic effect helps to improve the utilization rate and control effect of pesticides and reduce the loss and waste of pesticides; 2. The present application can be formulated into suspension concentrate, emulsifiable concentrate and water dispersible granules to meet different agricultural production needs. DETAILED DESCRIPTION

[0044] The raw materials in this application include the following parts: Metconazole: a commercially available product with CAS number 125116-23-6 was used; Prochloraz: a commercial product with CAS number 67747-09-5 was used; Fatty alcohol polyoxyethylene ether sulfate: There are many kinds of fatty alcohol polyoxyethylene ether sulfate, including sodium fatty alcohol polyoxyethylene ether sulfate, ammonium fatty alcohol polyoxyethylene ether sulfate, etc. This application uses the commercially available product sodium fatty alcohol polyoxyethylene ether sulfate with CAS number 9004-82-4 as an example; Naphthaldehyde condensate sulfonate: There are many kinds of naphthaldehyde condensate sulfonate, including sodium naphthaldehyde condensate sulfonate, potassium naphthaldehyde condensate sulfonate, etc. This application uses the commercially available product of sodium naphthaldehyde condensate sulfonate with CAS No. 36290-04-7 as an example; Polyacrylate: There are many types of polyacrylate, including sodium polyacrylate, potassium polyacrylate, etc. This application uses a commercially available product of sodium polyacrylate with a CAS number of 9003-04-7 as an example; Sodium lauryl sulfate: a commercially available product with CAS number 12738-53-3; Fatty alcohol polyoxyethylene ether: a commercially available product with CAS number 68511-39-7; Nikkomycin Z: a ​​commercially available product with CAS number 59456-70-1 was used; The present application is further described in detail below in conjunction with embodiments and comparative examples.

[0045] Example 1 A method for preparing a bactericidal composition containing metconazole and prochloraz, comprising the following steps: 100 mg of metconazole, 250 mg of prochloraz, 50 mg of sodium naphthaldehyde condensate sulfonate and 30 mg of sodium dodecyl sulfate were dissolved in methanol and the volume was fixed to 100 mL to prepare a 1000 mg / L mother solution to obtain a fungicide composition containing metconazole and prochloraz.

[0046] Example 2-3 Example 2-3 Based on the preparation method of Example 1, the content of each component of the fungicidal composition containing metconazole and prochloraz was adjusted, and the specific adjustment is shown in Table 1.

[0047] Comparative Examples 1-3 Comparative Examples 1-3 Based on the preparation method of Example 1, the contents of the components of the fungicidal composition containing metconazole and prochloraz were adjusted, and the specific adjustments are shown in Table 1.

[0048] Table 1 Contents of components and performance test of the fungicidal compositions containing metconazole and prochloraz in Examples 1-3 and Comparative Examples 1-3 Performance Test The bactericidal compositions containing metconazole and prochloraz in Examples 1-3 and Comparative Examples 1-3 were sampled and subjected to the following performance tests. The test results are shown in Table 1.

[0049] 1. Indoor toxicity determination of wheat fusarium wilt The EC values ​​of metconazole, prochloraz and each fungicide combination were calculated using the NYT1156.2-2006 Pesticide Indoor Bioassay Guidelines for Fungicides Part 2. 50 The DPS statistical software was used to perform regression analysis on the logarithmic values ​​of the treatment concentrations and the corresponding inhibition rate probability values ​​of each single agent and different mixed combinations, and the toxicity regression curve and correlation coefficient were calculated. The co-toxicity coefficient of the mixture was calculated according to the Sun Yunpei method. The co-toxicity coefficient (CTC) of the compound combination ≥120 is a synergistic effect; CTC≤80 is an antagonistic effect; 80<CTC<120 is an additive effect. In this experiment, wheat fusarium was used, and the concentrations of the fungicidal composition were 0.03125, 0.0625, 0.125, 0.25, 0.5, and 1 mg / L, respectively.

[0050] 2. Indoor virulence determination of wheat rust The EC values ​​of metconazole, prochloraz and each fungicide combination were calculated using the NYT1156.2-2006 Pesticide Indoor Bioassay Guidelines for Fungicides Part 2. 50The DPS statistical software was used to perform regression analysis on the logarithmic values ​​of the treatment concentrations and the corresponding inhibition rate probability values ​​of each single agent and different mixed combinations, and the toxicity regression curve and correlation coefficient were calculated. The co-toxicity coefficient of the mixture was calculated according to the Sun Yunpei method. The co-toxicity coefficient (CTC) of the compound combination ≥ 120 is a synergistic effect; CTC ≤ 80 is an antagonistic effect; 80 < CTC < 120 is an additive effect. Wheat stripe rust was used in this experiment, and the concentrations of the fungicidal composition were 0.625, 1.25, 2.5, 5, 10, and 20 mg / L, respectively.

[0051] 3. Indoor toxicity determination of wheat powdery mildew The EC values ​​of metconazole, prochloraz and each fungicide combination were calculated using the NYT1156.2-2006 Pesticide Indoor Bioassay Guidelines for Fungicides Part 2. 50 The DPS statistical software was used to perform regression analysis on the logarithmic values ​​of the treatment concentrations and the corresponding inhibition rate probability values ​​of each single agent and different mixed combinations, and the toxicity regression curve and correlation coefficient were calculated. The co-toxicity coefficient of the mixture was calculated according to the Sun Yunpei method. The co-toxicity coefficient (CTC) of the compound combination ≥ 120 is a synergistic effect; CTC ≤ 80 is an antagonistic effect; 80 < CTC < 120 is an additive effect. In this experiment, wheat powdery mildew fungus was used, and the concentrations of the fungicidal composition were 0.625, 1.25, 2.5, 5, 10, and 20 mg / L, respectively.

[0052] Referring to Table 1, it can be seen from the comparison between Examples 1-3 and Comparative Examples 1-3 that the EC50 values ​​of the compositions of Examples 1-3 for fusarium head blight are lower than the EC50 values ​​of fusarium head blight of metconazole and prochloraz alone, and the co-toxicity coefficients of fusarium head blight show a synergistic effect. The EC50 values ​​of the compositions of Comparative Examples 1-3 for fusarium head blight are between the EC50 values ​​of fusarium head blight of metconazole and prochloraz alone, and the co-toxicity coefficients of fusarium head blight show an additive effect. This indicates that the compound of metconazole and prochloraz has a good control effect on wheat fusarium head blight, and the content of prochloraz in the compound is greater than that of metconazole, which is more conducive to improving the control effect.

[0053] The EC50 values ​​of rust of the compositions of Examples 1-3 are slightly higher than the EC50 values ​​of metconazole alone, and much lower than the EC50 values ​​of prochloraz alone, and the co-toxicity coefficients of rust all show synergistic effects. Although the EC50 values ​​of rust of Comparative Examples 1-3 are also slightly higher than the EC50 values ​​of metconazole alone, and much lower than the EC50 values ​​of prochloraz alone, the co-toxicity coefficients show an additive effect. This shows that the combination of metconazole and prochloraz has a good control effect on wheat rust, and the content of prochloraz in the combination is greater than that of metconazole, which is more conducive to improving the control effect.

[0054] The EC50 values ​​of the powdery mildew of the compositions of Examples 1-3 are slightly higher than the EC50 values ​​of metconazole alone, and much lower than the EC50 values ​​of prochloraz alone, and the co-toxicity coefficients of powdery mildew all show synergistic effects. Although the EC50 values ​​of powdery mildew of Comparative Examples 1-3 are also slightly higher than the EC50 values ​​of metconazole alone, and much lower than the EC50 values ​​of prochloraz alone, the co-toxicity coefficients show an additive effect. This indicates that the combination of metconazole and prochloraz has a good control effect on wheat powdery mildew, and the content of prochloraz in the combination is greater than that of metconazole, which is more conducive to improving the control effect.

[0055] In addition, by comparing Examples 1-3, it was found that the co-toxicity coefficients of the three diseases in Example 1 were the highest, the EC50 value of fusarium head blight was the lowest, and the EC50 values ​​of rust and powdery mildew were the second lowest. Therefore, considering comprehensively, Example 1 is preferred.

[0056] Embodiment 4-7 In Example 4-7, based on the preparation method of Example 1, the amount of prochloraz added was adjusted. The specific adjustments are shown in Table 2.

[0057] The fungicidal compositions containing metconazole and prochloraz in Examples 4-7 were sampled and subjected to the above performance tests. The test results are shown in Table 2.

[0058] Table 2 The amount of prochloraz added and the performance test table of Example 1 and Examples 4-7 Referring to Table 2, by comparing Example 1 with Examples 4-7, it can be seen that as the amount of prochloraz added increases, the co-toxicity coefficients of the three diseases all show a trend of first increasing and then decreasing. This may be because as the amount of prochloraz added increases, the synergistic effect of prochloraz and metconazole increases, thereby increasing the co-toxicity coefficient of the three diseases of the fungicidal composition containing metconazole and prochloraz; when the amount of prochloraz added exceeds a certain range, it will inevitably lead to a relative decrease in the content of metconazole, which is not conducive to the synergistic effect of the two, thereby reducing the co-toxicity coefficient of the three diseases of the fungicidal composition containing metconazole and prochloraz.

[0059] Embodiment 8-11 In Examples 8-11, based on the preparation method of Example 1, the amount of sodium naphthalene formaldehyde condensate sulfonate added was adjusted. The specific adjustments are shown in Table 3.

[0060] The fungicidal compositions containing metconazole and prochloraz in Examples 8-11 were sampled and subjected to the above performance tests. The test results are shown in Table 3.

[0061] Table 3 Addition amount and performance test table of naphthalene formaldehyde condensate sodium sulfonate of Example 1 and Example 8-11 Referring to Table 3, it can be seen from the comparison between Example 1 and Examples 8-11 that as the amount of sodium sulfonate of condensate of naphthaldehyde increases, the EC 50 The values ​​all showed a trend of first decreasing and then increasing, and the co-toxicity coefficients of the three diseases all showed a trend of first increasing and then decreasing. This may be because as the amount of sodium naphthaldehyde condensate sulfonate added continued to increase, the dispersion force was gradually improved, the aggregation of prochloraz particles was avoided, the uniformity and stability of the preparation were improved, and the EC values ​​of the three diseases of the fungicidal composition containing metconazole and prochloraz were reduced. 50 value, and improve the co-toxicity coefficient of the three diseases of the fungicidal composition containing metconazole and prochloraz; when the addition amount of sodium naphthaldehyde condensate sulfonate exceeds a certain range, it may cause the system to be too viscous, increase the fluidity resistance of the preparation, and even affect the dispersion effect of the preparation, thereby improving the EC of the three diseases of the fungicidal composition containing metconazole and prochloraz 50 value, and reduce the co-toxicity coefficient of the three diseases of the fungicidal composition containing metconazole and prochloraz.

[0062] Examples 12-17 Example 12 Based on the preparation method of Example 1, 50 mg of sodium naphthalene formaldehyde condensate sulfonate was replaced with 60 mg of sodium polyacrylate, and the other conditions remained unchanged.

[0063] In Examples 13-17, based on the preparation method of Example 12, the amount of sodium polyacrylate added was adjusted. The specific adjustments are shown in Table 4.

[0064] The fungicidal compositions containing metconazole and prochloraz in Examples 12-17 were prepared and subjected to the above performance tests. The test results are shown in Table 4.

[0065] Table 4 Addition amount and performance test table of sodium polyacrylate in Example 1 and Examples 12-17 Referring to Table 4, it can be seen from the comparison between Example 1 and Examples 12-17 that sodium polyacrylate can also be used in the present application.

[0066] With the increasing amount of sodium polyacrylate added, the EC of the three diseases 50 The values ​​all showed a trend of first decreasing and then increasing, and the co-toxicity coefficients of the three diseases all showed a trend of first increasing and then decreasing. This may be because as the amount of sodium polyacrylate added continued to increase, the dispersion force was gradually improved, the aggregation of prochloraz particles was avoided, the uniformity and stability of the preparation were improved, and the EC values ​​of the three diseases of the fungicidal composition containing metconazole and prochloraz were reduced. 50value, and improve the co-toxicity coefficient of the three diseases of the fungicidal composition containing metconazole and prochloraz; when the addition amount of sodium polyacrylate exceeds a certain range, it may cause the system to be too viscous, increase the fluidity resistance of the preparation, and even affect the dispersion effect of the preparation, thereby improving the EC of the three diseases of the fungicidal composition containing metconazole and prochloraz 50 value, and reduce the co-toxicity coefficient of the three diseases of the fungicidal composition containing metconazole and prochloraz.

[0067] Examples 17-21 Example 17 Based on the preparation method of Example 1, 50 mg of sodium naphthalene formaldehyde condensate sulfonate and 30 mg of sodium dodecyl sulfate are removed and replaced with 110 mg of sodium polyacrylate, and the other conditions remain unchanged.

[0068] In Examples 18-21, based on the preparation method of Example 17, the amount of fatty alcohol polyoxyethylene ether sodium sulfate added was adjusted. The specific adjustments are shown in Table 5.

[0069] The fungicidal compositions containing metconazole and prochloraz in Examples 17-21 were sampled and subjected to the above performance tests. The test results are shown in Table 5.

[0070] Table 5 Addition amount and performance test table of fatty alcohol polyoxyethylene ether sodium sulfate of Example 1 and Examples 17-21 Referring to Table 5, it can be seen from the comparison between Example 1 and Examples 17-21 that sodium fatty alcohol polyoxyethylene ether sulfate can also be used in the present application, and sodium fatty alcohol polyoxyethylene ether sulfate can be used as both a dispersant and a wetting agent.

[0071] With the increasing addition of sodium sulfate of fatty alcohol polyoxyethylene ether, the EC of the three diseases 50 The values ​​all showed a trend of first decreasing and then increasing, and the co-toxicity coefficients of the three diseases all showed a trend of first increasing and then decreasing. This may be because as the amount of fatty alcohol polyoxyethylene ether sodium sulfate added continued to increase, the dispersion force was gradually improved, the aggregation of prochloraz particles was avoided, the uniformity and stability of the preparation were improved, and the EC values ​​of the three diseases of the fungicidal composition containing metconazole and prochloraz were reduced. 50 value, and improve the co-toxicity coefficient of the three diseases of the fungicidal composition containing metconazole and prochloraz; when the addition amount of sodium polyoxyethylene fatty alcohol ether sulfate exceeds a certain range, it may cause excessive foaming, decreased stability and other problems; sodium polyoxyethylene fatty alcohol ether sulfate, as a surfactant, may form micelles or aggregates after reaching a certain concentration, which in turn affects its adsorption and dispersion effect on the surface of pesticide particles, thereby improving the EC of the three diseases of the fungicidal composition containing metconazole and prochloraz. 50value, and reduce the co-toxicity coefficient of the three diseases of the fungicidal composition containing metconazole and prochloraz.

[0072] Examples 22-25 In Examples 22-25, based on the preparation method of Example 1, the amount of sodium dodecyl sulfate added was adjusted. The specific adjustments are shown in Table 6.

[0073] The fungicidal compositions containing metconazole and prochloraz in Examples 22-25 were prepared and subjected to the above performance tests. The test results are shown in Table 6.

[0074] Table 6 Addition amount and performance test table of sodium lauryl sulfate in Example 1 and Examples 22-25 Referring to Table 6, by comparing Example 1 with Examples 22-25, it can be seen that as the amount of sodium dodecyl sulfate added increases, the EC 50 The values ​​all showed a trend of first decreasing and then increasing, and the co-toxicity coefficients of the three diseases all showed a trend of first increasing and then decreasing. This may be because as the amount of sodium dodecyl sulfate added continued to increase, the wetting ability gradually increased, so that the preparation gradually formed a uniform covering layer on the target surface, promoting the absorption and efficacy of metconazole and myclobutanil, thereby reducing the EC50 values ​​of the three diseases of the fungicidal composition containing metconazole and myclobutanil, and increasing the co-toxicity coefficients of the three diseases of the fungicidal composition containing metconazole and myclobutanil; when the amount of sodium dodecyl sulfate added exceeded a certain range, it may cause the preparation to form too much wetting layer on the target surface, and even cause flowing or dripping, which not only wastes the agent, but also may cause a large amount of agent to accumulate on the bottom leaves, which is easy to cause phytotoxicity, thereby increasing the EC50 values ​​of the three diseases of the fungicidal composition containing metconazole and myclobutanil. 50 value, and reduce the co-toxicity coefficient of the three diseases of the fungicidal composition containing metconazole and prochloraz.

[0075] Examples 26-30 Example 26 Based on the preparation method of Example 1, 30 mg of sodium lauryl sulfate is replaced with 35 mg of fatty alcohol polyoxyethylene ether, and the other conditions remain unchanged.

[0076] In Examples 27-30, based on the preparation method of Example 21, the amount of fatty alcohol polyoxyethylene ether added was adjusted. The specific adjustments are shown in Table 7.

[0077] The fungicidal compositions containing metconazole and prochloraz in Examples 26-30 were prepared and subjected to the above performance tests. The test results are shown in Table 7.

[0078] Table 7 Addition amount and performance test table of fatty alcohol polyoxyethylene ether of Example 1 and Examples 26-30 Referring to Table 7, it can be seen from the comparison between Example 1 and Examples 26-30 that fatty alcohol polyoxyethylene ether can also be used in the present application.

[0079] As the addition amount of fatty alcohol polyoxyethylene ether increases, the EC50 values ​​of the three diseases all show a trend of first decreasing and then stabilizing, and the co-toxicity coefficients of the three diseases all show a trend of first increasing and then stabilizing. This may be because as the addition amount of fatty alcohol polyoxyethylene ether increases, the wetting ability gradually increases, so that the preparation gradually forms a uniform covering layer on the target surface, promoting the absorption and efficacy of metconazole and myclobutanil, thereby reducing the EC50 values ​​of the three diseases of the fungicidal composition containing metconazole and myclobutanil, and increasing the co-toxicity coefficient of the three diseases of the fungicidal composition containing metconazole and myclobutanil.

[0080] Examples 31-32 Example 31 Based on the preparation method of Example 1, 50 mg of nikkomycin Z was added, and the other conditions remained unchanged.

[0081] Example 32 Based on the preparation method of Example 1, 100 mg of nikkomycin Z was added, and the other conditions remained unchanged.

[0082] The fungicidal compositions containing metconazole and prochloraz of Examples 31-32 were sampled and subjected to the above performance tests. The test results are shown in Table 7.

[0083] Table 8 Addition amount and performance test table of nikkomycin Z in Example 1 and Examples 31-32 Referring to Table 8, by comparing Example 1 with Examples 31-32, it can be seen that adding nikkomycin Z to the composition can improve the control effect of the composition on wheat fusarium head blight, rust and powdery mildew.

[0084] Nikkomycin Z can effectively block the synthesis pathway of chitin and destroy the cell wall. Methconazole and prochloraz interfere with the formation and function of the cell membrane by affecting the synthesis of ergosterol. After nikkomycin Z destroys the cell wall of the pathogen, it is more conducive to metconazole and prochloraz to destroy the cell membrane of the pathogen. The obstruction of chitin synthesis caused by nikkomycin Z will cause changes in the cytoplasmic osmotic pressure of the pathogen. Since the cell membrane has been affected by metconazole and prochloraz, its ability to resist external pressure is weakened, so this change in osmotic pressure will further aggravate cell damage. The mutual synergy of nikkomycin Z, metconazole and prochloraz can improve the control effect of the composition on wheat fusarium head blight, rust and powdery mildew.

[0085] Application Examples The above-mentioned Examples 1-34 can all be prepared into the form of suspensions, emulsifiable concentrates and water-dispersible granules. The following application examples are exemplified based on the basic formula of Example 1.

[0086] The emulsifier may be fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene ether, benzylphenol polyoxyethylene ether, etc., and fatty alcohol polyoxyethylene ether is used as an example in this application.

[0087] The antifreeze agent may be propylene glycol, ethylene glycol, sorbitol, etc., and the present application uses a commercially available propylene glycol product with a CAS number of 57-55-6 as an example.

[0088] The defoaming agent can be selected from organic silicone, C8-10 fatty alcohol, C19-20 saturated aliphatic carboxylic acid, etc. The present application uses commercially available organic silicone products for illustration.

[0089] The thickener may be xanthan gum, gum arabic, methyl cellulose, etc. This application uses commercially available xanthan gum as an example.

[0090] Preservatives may be selected from kason, sodium benzoate, sorbic acid, etc., and this application uses commercially available kason as an example.

[0091] The organic solvent may be cyclohexanone, xylene, N,N-dimethylformamide, etc. The present application uses a commercially available cyclohexanone product with a CAS number of 108-94-1 as an example.

[0092] The filler may be corn starch, kaolin, talcum powder, etc., and this application uses corn starch and kaolin as examples.

[0093] Preparation Example 1 The formula is: 100g of metconazole, 250g of prochloraz, 50g of naphthaldehyde condensate sulfonate and 30g of sodium lauryl sulfate, 50g of propylene glycol, 2g of silicone, 2g of xanthan gum, 2g of kason, and water is added to 1000g.

[0094] Preparation method: Add other auxiliary agents except the thickener xanthan gum into water and mix them evenly by high-speed shearing, add the effective ingredients metconazole and prochloraz, mix evenly, pass through a sand mill with zirconium beads as grinding media to control the particle diameter to less than 5 μm, then add the thickener and shear evenly to obtain the metconazole-prochloraz suspension.

[0095] Preparation Example 2 The formula is: 100g of metconazole, 250g of prochloraz, 50g of naphthaldehyde condensate sulfonate and 30g of sodium lauryl sulfate, 11g of fatty alcohol polyoxyethylene ether, 50g of propylene glycol, 2g of silicone, 2g of xanthan gum, 2g of kason, and cyclohexanone is supplemented to 1000g.

[0096] Preparation method: firstly, dissolve metconazole and prochloraz in the organic solvent cyclohexanone, then slowly add the remaining auxiliary agents in batches, and shear at high speed to form a homogeneous transparent oily liquid to obtain metconazole-prochloraz emulsifiable concentrate.

[0097] Preparation Example 3 Formula: 100g metconazole, 250g prochloraz, 50g naphthaldehyde condensate sulfonate and 30g sodium lauryl sulfate, 240g corn starch, and kaolin is added to make up to 1000g.

[0098] Preparation method: First, the ingredients are stirred and mixed in a mixing kettle, crushed by a jet mill, mixed evenly, kneaded, granulated, dried, sieved, and sampled for analysis to obtain metconazole-prochloraz water-dispersible granules.

[0099] Comparative Example 4 Comparative Example 4 is a 50% metconazole water dispersible granule purchased on the market Comparative Example 5 Comparative Example 5 is a 25% prochloraz emulsifiable concentrate purchased on the market.

[0100] Preparation Examples 1-3 and Comparative Examples 4-5 were used for field control of the following wheat fusarium, wheat rust and wheat powdery mildew, and the results obtained are shown in Tables 9-11.

[0101] 1. Prevention and control of wheat fusarium wilt Referring to the "Guidelines for Field Efficacy Tests of Pesticides Part 15: Fungicide Control of Wheat Fusarium Head Blight" (NY / T1464.15-2007), the following dosage of original pesticide is diluted in 40 liters of water for spraying on wheat in each acre of area.

[0102] 2. Prevention and control of wheat rust With reference to NY / T2885-2016 "Quality Management Specifications for Field Efficacy Tests for Pesticide Registration" and GB / T17980.23-2000 "Guidelines for Field Efficacy Tests of Pesticides (I) Fungicides for Control of Cereal Rust (Leaf Rust, Stripe Rust, Stalk Rust)", the following dosage of original pesticide is diluted in 40 liters of water for spraying on wheat in each acre of area.

[0103] 3. Prevention and control of wheat powdery mildew With reference to the Guidelines for Field Efficacy Tests of Pesticides (I) Fungicides for Control of Powdery Mildew of Cereals (GB / T17980.22-2000), the following dosage of original pesticide is diluted in 40 liters of water for spraying on wheat in each acre of area.

[0104] Table 9 Field test results of control on wheat scab Table 10 Field test effect of control on wheat rust Table 11 Field test effect of control on wheat powdery mildew Referring to Tables 9-11, by comparing Preparation Examples 1-3, Comparative Examples 4-5 and the blank control, it can be seen that the disease indexes of Preparation Examples 1-3 are lower than those of Comparative Examples 4-5 and the blank control, and the prevention effects are higher than those of Comparative Examples 4-5 and the blank control, indicating that the fungicidal composition containing metconazole and prochloraz can effectively exert its effect regardless of being made into a suspension, emulsifiable concentrate or water-dispersible granules.

[0105] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A fungicidal composition containing metconazole and prochloraz, characterized in that: The invention comprises the following components in parts by weight: 10 parts of metconazole, 10-40 parts of prochloraz, 2-10 parts of a dispersant, and 1-5 parts of a wetting agent; The dispersant includes at least one of fatty alcohol polyoxyethylene ether sulfate, naphthaldehyde condensate sulfonate, and polyacrylic acid salt; The wetting agent includes at least one of fatty alcohol polyoxyethylene ether sulfate, sodium lauryl sulfate, and fatty alcohol polyoxyethylene ether.

2. The fungicidal composition containing metconazole and prochloraz according to claim 1, characterized in that: The mass ratio of the metconazole to the prochloraz is 1:2-3.

3. The fungicidal composition containing metconazole and prochloraz according to claim 1, characterized in that: The mass ratio of the prochloraz to the fatty alcohol polyoxyethylene ether sulfate is 25:8-12.

4. The fungicidal composition containing metconazole and prochloraz according to claim 1, characterized in that: The mass ratio of the prochloraz to the naphthaldehyde condensate sulfonate is 25:3-6.

5. The fungicidal composition containing metconazole and prochloraz according to claim 1, characterized in that: The mass ratio of prochloraz to sodium lauryl sulfate is 25:2-4.

6. The fungicidal composition containing metconazole and prochloraz according to claim 1, characterized in that: The mass ratio of prochloraz to fatty alcohol polyoxyethylene ether is 25:1.5-3.

5.

7. The fungicidal composition containing metconazole and prochloraz according to claim 1, characterized in that: Nikkomycin Z is also included.

8. The use of the bactericidal composition containing metconazole and prochloraz in any one of claims 1 to 7, characterized in that: Applications include the prevention and control of wheat fusarium rust, wheat rust and wheat powdery mildew.

9. The use of the bactericidal composition containing metconazole and prochloraz according to claim 8, characterized in that: The bactericidal composition containing metconazole and prochloraz is formulated to include at least one of a suspension concentrate, an emulsifiable concentrate and a water-dispersible granule.

10. The use of the fungicidal composition containing metconazole and prochloraz according to claim 9, characterized in that: When the fungicidal composition containing metconazole and prochloraz is formulated into emulsifiable concentrate, an emulsifier needs to be added, and the emulsifier includes fatty alcohol polyoxyethylene ether.

Images