A liquid-based thin-layer slide making apparatus and method

Abstract

The application discloses a liquid-based thin-layer sample preparation device and a sample preparation method, which comprise a main base, a sample preparation machine box is fixedly arranged on one side of the top end of the main base, a guiding sample preparation mechanism is fixedly arranged on the front face of the sample preparation machine box, a machine box cover is hingedly arranged on one side of the top end of the sample preparation machine box, a transfer cup seat is fixedly arranged on one side of the top end of the machine box cover and faces the guiding sample preparation mechanism, a filter cup seat is fixedly arranged on the other side of the top end of the sample preparation machine box, and a disposable membrane cup is clampedly connected to the inner side of the filter cup seat. The liquid-based thin-layer sample preparation device and the sample preparation method separate and step-by-step operate cell filtering and adsorbing and transfer sample preparation, filter and transfer by using the disposable membrane cup, do not need to clean and disinfect the sample preparation device after single sample preparation, operation and use are more convenient, the operation process of the staff is reduced, the disposable membrane cup is used, sample infection in the sample preparation process can be effectively avoided, and the accuracy of screening can be ensured.

Description

Technical Field

[0001] The present invention relates to the technical field of liquid-based thin layer film making, in particular to a liquid-based thin layer film making device and a film making method. Background Art

[0002] Liquid-based thin-layer cytology (LTC) is a cervical cytology test, also known as cervical TCT. It is primarily used for cervical cytology and can also be used for cervical cancer screening. TCT examines cervical squamous cells for abnormalities. If atypical squamous cells are present, further biopsy or follow-up examination after a period of observation is necessary. This test can detect early cervical lesions. In medical systems, to improve the efficiency of cytology preparation, liquid-based thin-layer cytology equipment is often used for preparation.

[0003] Common preparation equipment, such as the preparation mechanism of the fully automatic liquid-based thin layer cell preparation and staining machine with application number 202011158668.0, uses rotary centrifugation and suction to achieve cell separation and preparation. Although it can improve the efficiency of preparation, the preparation mechanism needs to be frequently cleaned and disinfected after use, and cross-infection is prone to occur during preparation, which is not conducive to improving the accuracy of screening.

[0004] The existing production equipment has a complicated process, which places a heavy workload on staff. At the same time, cross-infection is prone to occur during production, which is not conducive to improving the accuracy of screening. Summary of the Invention

[0005] The purpose of the present invention is to provide a liquid-based thin layer preparation device and preparation method to solve the problems raised in the above background technology that the existing preparation equipment has a cumbersome use process, a large workload for staff, and is prone to cross-infection during preparation, which is not conducive to improving the accuracy of screening.

[0006] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a liquid-based thin layer film-making equipment, comprising a main base, a film-making box is fixedly provided on one side of the top of the main base, a film-making mechanism is fixedly provided on the front of the film-making box, a box cover is hingedly provided on one side of the top of the film-making box, a transfer cup seat is fixedly provided on one side of the top of the box cover and faces the guiding film-making mechanism, a filter cup seat is fixedly provided on the other side of the top of the film-making box, a disposable membrane cup is snap-connected to the inner side of the filter cup seat, a membrane cup suction cup seat is fixedly provided on the bottom of the inner wall of the film-making box and faces the filter cup seat, a negative suction device is fixedly installed on one side of the membrane cup suction cup seat, a water outlet of the negative suction device is fixedly connected to a liquid storage tank through a pipeline, a controller is fixedly installed inside the main base, a control panel is fixedly installed on the other side of the top of the main base, and a display and an on-off switch are fixedly installed on the surface of the control panel.

[0007] Preferably, the guiding film-making mechanism includes a guide plate and a slide fixing frame, a transmission groove is opened in the middle of the top of the guide plate, the internal rotation of the transmission groove is connected to a transmission screw, the middle of the transmission screw is connected to a transmission slider, the top of the transmission slider is fixedly provided with a slide fixing frame, one end of the guide plate is fixedly installed with a reduction motor, the output end of the reduction motor is fixedly connected to one end of the transmission screw, and the reduction motor is electrically connected to the controller.

[0008] Preferably, a negative suction cavity is provided inside the glass slide holder, a scratch-resistant rubber pad is laid on the bottom of the inner wall of the glass slide holder, a plurality of negative suction holes communicating with the interior of the negative suction cavity are provided on the surface of the scratch-resistant rubber pad, a pressure sensor is fixedly installed on one end of the inner wall of the glass slide holder, a small vacuum pump is fixedly installed on one end of the glass slide holder, an air inlet of the small vacuum pump is fixedly connected to a negative suction pipe, one end of the negative suction pipe is fixedly connected to the interior of the negative suction cavity, and the small vacuum pump and the pressure sensor are both electrically connected to the controller.

[0009] Preferably, the disposable membrane cup includes an upper membrane cup and a lower membrane cup, the bottom end of the upper membrane cup is provided with a lower membrane cup, the top end of the lower membrane cup is fixedly provided with a transfer adsorption film, and the bottom end of the upper membrane cup is fixedly provided with a filter screen.

[0010] Preferably, indicator lights are fixedly installed on one side of the transfer cup holder and one side of the filter cup holder, and the two indicator lights and the negative suction device are electrically connected to the controller, and the controller is electrically connected to the external power supply through an on-off switch.

[0011] Preferably, fixed bases are fixedly installed on both sides of the film-making machine chassis, and fixed buckles corresponding to the fixed bases are fixedly provided on both sides of the chassis cover.

[0012] Preferably, anti-slip pads are fixedly provided on the four corners of the bottom end of the main frame.

[0013] A liquid-based thin layer preparation method comprises the following steps:

[0014] S1. Sampling and collection: Use a sampling brush to collect cells from the patient according to the conventional sampling process. After sampling, place the sampling brush in a preservation bottle. Add an appropriate amount of preservation treatment solution to the preservation bottle in advance and shake it thoroughly to ensure that the sampled cells on the sampling brush can be fully dispersed in the preservation treatment solution. Then remove the sampling brush;

[0015] S2. Centrifugal separation: Place the sampled storage bottle in a centrifuge for centrifugal stratification, aspirate and remove non-diagnostic impurities such as mucus in the sample, then transfer the processed sample cells to a new container, add an appropriate amount of buffer, dilute the cell mass, and let it stand for 15-20 minutes after dilution;

[0016] S3. Water control and adsorption: Take out the disposable membrane cup and place it on the filter cup holder. At this time, the lower membrane cup will be close to the membrane cup suction cup holder. Use the control panel to control the negative suction device to work. Then pour the sample cells and buffer after standing into the upper membrane cup. Under the action of negative suction, the debris in the sample will be filtered through the filter mesh, and the buffer will pass through the transfer adsorption membrane into the liquid storage tank, while the sample cells will remain on the transfer adsorption membrane.

[0017] S4. Assemble the slide: Insert the clean slide into the slide holder. When the slide presses the pressure sensor, the small vacuum pump will work, continuously sucking the air in the negative suction chamber, forming negative pressure at the negative suction hole, firmly adsorbing the slide. Then the reduction motor will drive the entire slide holder to move through the transmission screw and transmission slider until the slide smear position is facing the transfer cup seat.

[0018] S5. Transfer and preparation: After the two indicator lights light up, take out the disposable membrane cup, separate the upper membrane cup and the lower membrane cup, then insert the lower membrane cup upside down into the transfer cup holder and press it. At this time, the cells on the transfer adsorption membrane will be transferred to the slide. After the transfer is completed, remove the lower membrane cup and discard the disposable membrane cup into the medical waste bin;

[0019] S6. Eject the slide: Use the control panel to stop the small vacuum pump, reverse the deceleration motor, and eject the slide.

[0020] S7. Sealing: Slowly take out the prepared slide, fix it in 95% alcohol, and then stick the sealing slide to fix it.

[0021] Preferably, the preservation treatment solution is prepared by mixing physiological saline, ethanol, sodium citrate, and acetylcysteine ​​in a ratio of 6:2:1:1.

[0022] Preferably, the buffer is prepared by mixing physiological saline, ethanol, Papanicolaou stain, sodium citrate and acetylcysteine.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] 1. The liquid-based thin layer preparation device has a simple structural design, and separates cell filtration adsorption and transfer preparation into separate steps. Disposable membrane cups are used for filtration and transfer. There is no need to clean and disinfect the preparation device after a single preparation. The operation is more convenient and reduces the operating procedures of the staff. The use of disposable membrane cups can effectively avoid sample infection during the preparation process and ensure the accuracy of screening.

[0025] 2. The preparation process is simple and convenient. Cells can be stained during the preparation process, making the cells clearer and less likely to overlap. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a structural schematic diagram of the film-making equipment of the present invention;

[0027] Figure 2 It is a structural diagram of the film-making guide mechanism of the present invention;

[0028] Figure 3 Schematic diagram of the structure of the glass slide fixing frame of the present invention;

[0029] Figure 4 is a front cross-sectional view of the slide holder of the present invention;

[0030] Figure 5 is a side sectional view of the tableting apparatus of the present invention;

[0031] Figure 6 It is a front cross-sectional view of the disposable membrane cup of the present invention;

[0032] Figure 7 It is a flow chart of the film-making method of the present invention.

[0033] In the figure: 1. Main base; 2. Film making machine case; 3. Disposable membrane cup; 31. Upper membrane cup; 32. Lower membrane cup; 33. Filter; 34. Transfer adsorption film; 4. Control panel; 5. Guide film making mechanism; 51. Guide plate; 52. Negative suction chamber; 53. Slide fixing frame; 54. Negative suction hole; 55. Anti-scratch rubber pad; 56. Pressure sensor; 57. Transmission slide; 58. Transmission screw; 59. Reduction motor; 510. Small vacuum pump; 511. Negative suction pipe; 512. Transmission slider; 6. Case cover; 7. Transfer cup holder; 8. Indicator light; 9. Filter cup holder; 10. Fixing buckle; 11. Fixing base; 12. Anti-slip foot pad; 13. Membrane cup suction cup holder; 14. Controller; 15. Negative suction device; 16. Liquid storage tank. DETAILED DESCRIPTION

[0034] The technical solutions in the embodiments of the present invention will be described clearly and completely below with reference to the accompanying drawings in the embodiments of the present invention.

[0035] See also Figure 1-7The present invention provides a liquid-based thin layer film making device, comprising a main base 1, a film making machine case 2 is fixedly provided on one side of the top of the main base 1, a film making machine case 2 is fixedly provided on the front of the film making machine case 2, a case cover 6 is hingedly provided on one side of the top of the film making machine case 2, a transfer cup seat 7 facing the film making machine case 5 is fixedly provided on one side of the top of the case cover 6, a filter cup seat 9 is fixedly provided on the other side of the top of the film making machine case 2, a disposable membrane cup 3 is snap-connected to the inner side of the filter cup seat 9, and a membrane cup suction cup seat facing the filter cup seat 9 is fixedly provided on the bottom of the inner wall of the film making machine case 2 13. A negative suction device 15 is fixedly installed on one side of the membrane cup suction cup seat 13. The water outlet of the negative suction device 15 is fixedly connected to the liquid storage tank 16 through a pipe. A controller 14 is fixedly installed inside the main base 1. A control panel 4 is fixedly installed on the other side of the top of the main base 1. A display and an on-off switch are fixedly installed on the surface of the control panel 4. Fixed bases 11 are fixedly installed on both sides of the film making machine box 2. Fixed buckles 10 corresponding to the fixed base 11 are fixedly provided on both sides of the chassis cover 6. Anti-slip pads 12 are fixedly provided on the four corners of the bottom end of the main base 1.

[0036] The guide production mechanism 5 includes a guide plate 51 and a slide fixing frame 53. A transmission slide 57 is provided in the middle of the top of the guide plate 51. The transmission slide 57 is rotatably connected to a transmission screw 58. The middle of the transmission screw 58 is connected to a transmission slider 512. The top of the transmission slider 512 is fixedly provided with the slide fixing frame 53. A reduction motor 59 is fixedly installed at one end of the guide plate 51. The output end of the reduction motor 59 is fixedly connected to one end of the transmission screw 58. The reduction motor 59 is electrically connected to the controller 14. The interior of the slide fixing frame 53 is provided with a transmission screw 58. There is a negative suction chamber 52, and the bottom of the inner wall of the glass slide fixing frame 53 is paved with an anti-scratch rubber pad 55. The surface of the anti-scratch rubber pad 55 is provided with multiple negative suction holes 54 that are connected to the inside of the negative suction chamber 52. A pressure sensor 56 is fixedly installed at one end of the inner wall of the glass slide fixing frame 53, and a small vacuum pump 510 is fixedly installed at one end of the glass slide fixing frame 53. The air inlet of the small vacuum pump 510 is fixedly connected to a negative suction pipe 511, and one end of the negative suction pipe 511 is fixedly connected to the inside of the negative suction chamber 52. The small vacuum pump 510 and the pressure sensor 56 are both electrically connected to the controller 14.

[0037] The disposable membrane cup 3 includes an upper membrane cup 31 and a lower membrane cup 32. The lower membrane cup 32 is provided at the bottom end of the upper membrane cup 31. A transfer adsorption film 34 is fixedly provided at the top end of the lower membrane cup 32. A filter screen 33 is fixedly provided at the bottom end of the upper membrane cup 31. Indicator lights 8 are fixedly installed on one side of the transfer cup seat 7 and one side of the filter cup seat 9. The two indicator lights 8 and the negative suction device 15 are electrically connected to the controller 14. The controller 14 is electrically connected to the external power supply through the on-off switch.

[0038] A liquid-based thin layer preparation method comprises the following steps:

[0039] S1. Sampling and Collection: Use a sampling brush to collect cells from the patient according to the conventional sampling process. After sampling, place the sampling brush in a preservation bottle. Add an appropriate amount of preservation treatment solution to the preservation bottle in advance and shake it thoroughly to ensure that the sampled cells on the sampling brush can be fully dispersed in the preservation treatment solution. Then remove the sampling brush. The preservation treatment solution is made by mixing physiological saline, ethanol, sodium citrate, and acetylcysteine ​​in a ratio of 6:2:1:1;

[0040] S2. Centrifugation: Place the sampled storage bottle in a centrifuge for centrifugal stratification. Aspirate and remove non-diagnostic impurities such as mucus from the sample. Transfer the processed sample cells to a new container and add an appropriate amount of buffer to dilute the cell mass. After dilution, let it stand for 15-20 minutes. The buffer is a mixture of physiological saline, ethanol, Papanicolaou stain, sodium citrate, and acetylcysteine.

[0041] S3. Control water adsorption: Take out the disposable membrane cup 3 and place it on the filter cup holder 9. At this time, the lower membrane cup 32 will be close to the membrane cup suction cup holder 13. The negative suction device 15 is controlled by the control panel 4 to work. Then, the sample cells after standing together with the buffer are poured into the upper membrane cup 31. Under the action of negative suction, the debris in the sample will be filtered through the filter mesh 33, and the buffer will pass through the transfer adsorption membrane 34 into the liquid storage tank 16, while the sample cells will remain on the transfer adsorption membrane 34;

[0042] S4. Assemble the slide: Insert the clean slide onto the slide holder 53. When the slide presses the pressure sensor 56, the small vacuum pump 510 will start to work, continuously sucking the air in the negative suction chamber 52, forming a negative pressure at the negative suction hole 54, firmly adsorbing the slide. Then, the reduction motor 59 will drive the slide holder 53 to move as a whole through the transmission screw 58 and the transmission slider 512 until the slide smear position is directly opposite the transfer cup 7.

[0043] S5. Transfer and preparation: After the two indicator lights 8 light up, take out the disposable membrane cup 3, separate the upper membrane cup 31 and the lower membrane cup 32, then insert the lower membrane cup 32 upside down into the transfer cup holder 7 and press it. At this time, the cells on the transfer adsorption film 34 will be transferred to the glass slide. After the transfer is completed, remove the lower membrane cup 32 and discard the disposable membrane cup 3 into the medical waste bin;

[0044] S6. Ejecting the slide: Using the control panel 4, the small vacuum pump 510 stops working, the reduction motor 59 reverses, and the slide is ejected;

[0045] S7. Sealing: Slowly remove the slide after preparation, place it in 95% alcohol for fixation, and then stick the sealing slide for fixation. The preparation process is simple and convenient. Cells can be stained during the preparation process, making the cells clearer after preparation and less likely to overlap.

[0046] The cell filtration adsorption and transfer preparation are separated and operated in steps, and a disposable membrane cup 3 is used for filtration and transfer. There is no need to clean and disinfect the preparation equipment after a single preparation, which makes the operation more convenient and reduces the operating procedures of the staff. The use of the disposable membrane cup 3 can effectively avoid the phenomenon of sample infection in the preparation process, ensure the accuracy of screening, and make the preparation clear and efficient.

[0047] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A liquid-based thin layer film making device, comprising a main base (1), characterized in that: A tablet-making machine case (2) is fixedly provided on one side of the top of the main base (1), a tablet-making guide mechanism (5) is fixedly provided on the front of the tablet-making machine case (2), a case cover (6) is hingedly provided on one side of the top of the tablet-making machine case (2), a transfer cup seat (7) facing the tablet-making guide mechanism (5) is fixedly provided on one side of the top of the case cover (6), a filter cup seat (9) is fixedly provided on the other side of the top of the tablet-making machine case (2), and a disposable membrane cup (3) is snap-connected to the inner side of the filter cup seat (9). A membrane cup suction cup seat (13) facing the filter cup seat (9) is fixedly provided at the bottom of the inner wall of the film making machine box (2), a negative suction device (15) is fixedly installed on one side of the membrane cup suction cup seat (13), and the water outlet of the negative suction device (15) is fixedly connected to a liquid storage tank (16) through a pipe. A controller (14) is fixedly installed inside the main frame (1), and a control panel (4) is fixedly installed on the other side of the top of the main frame (1), and a display and an on / off switch are fixedly installed on the surface of the control panel (4); The guide film making mechanism (5) includes a guide plate (51) and a slide fixing frame (53), a transmission slot (57) is provided in the middle of the top of the guide plate (51), a transmission screw (58) is rotatably connected inside the transmission slot (57), a transmission slider (512) is transmission-connected in the middle of the transmission screw (58), a slide fixing frame (53) is fixedly provided on the top of the transmission slider (512), a reduction motor (59) is fixedly installed at one end of the guide plate (51), an output end of the reduction motor (59) is fixedly connected to one end of the transmission screw (58), and the reduction motor (59) is electrically connected to the controller (14); A negative suction cavity (52) is provided inside the glass slide holder (53), a scratch-resistant rubber pad (55) is provided at the bottom of the inner wall of the glass slide holder (53), a plurality of negative suction holes (54) communicating with the inner wall of the negative suction cavity (52) are provided on the surface of the scratch-resistant rubber pad (55), a pressure sensor (56) is fixedly installed at one end of the inner wall of the glass slide holder (53), a small vacuum pump (510) is fixedly installed at one end of the glass slide holder (53), an air inlet of the small vacuum pump (510) is fixedly connected to a negative suction pipe (511), one end of the negative suction pipe (511) is fixedly connected to the inner wall of the negative suction cavity (52), and both the small vacuum pump (510) and the pressure sensor (56) are electrically connected to the controller (14); The disposable membrane cup (3) comprises an upper membrane cup (31) and a lower membrane cup (32), wherein the lower membrane cup (32) is provided at the bottom end of the upper membrane cup (31), a transfer adsorption film (34) is fixedly provided at the top end of the lower membrane cup (32), and a filter screen (33) is fixedly provided at the bottom end of the upper membrane cup (31); An indicator light (8) is fixedly mounted on one side of the transfer cup holder (7) and one side of the filter cup holder (9). The two indicator lights (8) and the negative suction device (15) are electrically connected to a controller (14). The controller (14) is electrically connected to an external power supply via an on / off switch.

2. The liquid-based thin layer film making device according to claim 1, characterized in that: Both sides of the film-making machine box (2) are fixedly mounted with fixed bases (11), and both sides of the box cover (6) are fixedly provided with fixed buckles (10) corresponding to the fixed bases (11).

3. The liquid-based thin layer film making device according to claim 1, characterized in that: Anti-slip pads (12) are fixedly provided at the four corners of the bottom end of the main frame (1).

4. The method for producing a liquid-based thin layer film according to claim 1, wherein: The following steps are involved: S1. Sampling and collection: Use a sampling brush to collect cells from the patient according to the conventional sampling process. After sampling, place the sampling brush in a preservation bottle. Add an appropriate amount of preservation treatment solution to the preservation bottle in advance and shake it thoroughly to ensure that the sampled cells on the sampling brush can be fully dispersed in the preservation treatment solution. Then remove the sampling brush; S2. Centrifugal separation: Place the sampled storage bottle in a centrifuge for centrifugal stratification, aspirate and remove non-diagnostic impurities in the sample, then transfer the processed sample cells to a new container, add an appropriate amount of buffer, dilute the cell mass, and let it stand for 15-20 minutes after dilution; S3, water control adsorption: take out the disposable membrane cup (3), and put it on the filter cup seat (9). At this time, the lower membrane cup (32) will be close to the membrane cup suction cup seat (13). The negative suction device (15) is controlled to work through the control panel (4). Then, the sample cells after standing together with the buffer are poured into the upper membrane cup (31). Under the action of negative suction, the debris in the sample will be filtered through the filter mesh (33), and the buffer will pass through the transfer adsorption membrane (34) into the liquid storage tank (16), and the sample cells will stay on the transfer adsorption membrane (34); S4. Assembling the slide: insert the clean slide into the slide holder (53). When the slide presses the pressure sensor (56), the small vacuum pump (510) will work, continuously sucking the air in the negative suction chamber (52), forming a negative pressure at the negative suction hole (54), firmly adsorbing the slide. Then the reduction motor (59) will drive the slide holder (53) to move as a whole through the transmission screw (58) and the transmission slider (512) until the slide smear position is opposite to the transfer cup seat (7); S5, transfer preparation: After the two indicator lights (8) light up, take out the disposable membrane cup (3), separate the upper membrane cup (31) and the lower membrane cup (32), then insert the lower membrane cup (32) upside down into the transfer cup holder (7) and press it. At this time, the cells on the transfer adsorption film (34) will be transferred to the slide. After the transfer is completed, remove the lower membrane cup (32). At this time, the disposable membrane cup (3) can be discarded into the medical waste bin; S6. Ejecting the slide: Using the control panel (4), the small vacuum pump (510) stops working, the reduction motor (59) reverses, and the slide is ejected; S7. Sealing: Slowly take out the slide after preparation, fix it in 95% alcohol, and then stick the sealing slide to fix it.

5. The film-making method according to claim 4, wherein: The preservation treatment solution is prepared by mixing physiological saline, ethanol, sodium citrate, and acetylcysteine ​​in a ratio of 6:2:1:

1.

6. The film-making method according to claim 4, wherein: The buffer is prepared by mixing physiological saline, ethanol, Papanicolaou stain, sodium citrate and acetylcysteine.

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