Preparation method of Trichoderma gaimsii fertilizer specially used for garlic fruit seedlings

Abstract

The invention provides a method for preparing a special Gaims Trichoderma fertilizer for garlic fruit seedlings, the method comprising the steps of first preparing a high-concentration Gaims Trichoderma spore suspension, then preparing materials in proportion by using rice husk powder, wheat bran powder, corn stalk powder, magnesium sulfate, calcium phosphate and dipotassium hydrogen phosphate, sterilizing the materials by high pressure, adding the Gaims Trichoderma spore suspension, obtaining Gaims Trichoderma seeds with a high bacterial content after fermentation, and mixing and fermenting the rice husk powder, wheat bran powder, corn stalk powder and Gaims Trichoderma seeds to prepare the Gaims Trichoderma fertilizer. The Gaims Trichoderma fertilizer prepared by the method of the invention has a high bacterial content, is environmentally friendly and has a low production cost, has a significant growth-promoting effect on garlic fruit seedlings, and has good promotion and application value.

Description

Technical Field

[0001] The invention relates to the technical field of microbial fertilizers, and in particular to a special fertilizer for garlic fruit seedlings and a preparation method thereof. Background Art

[0002] Garlic fruit( Malania oleifera ) belongs to the genus Allium of the family Aspergillus. It is currently only naturally distributed in Guangnan and Funing counties in southeastern Yunnan and some limestone areas in western Guangxi. It is a national Class II rare and endangered protected plant and a key rescue and protection object for extremely small populations in Yunnan Province. The oil of the seed kernel of the Allium esculentum is rich in neuraminic acid, which can effectively promote the repair and regeneration of damaged nerve tissue, and therefore has important ecological and economic value. However, the natural regeneration of the Allium esculentum is difficult and the population is small. The afforestation survival rate and preservation rate of seedlings during artificial planting are low. Therefore, it is urgent to find a method to promote the growth of the Allium esculentum seedlings to improve the quality and survival rate of the seedlings.

[0003] Endophytic fungi are not only closely related to the nutrient absorption, growth and development, and survival of plants, but also play an important role in inducing plant systemic resistance. Previous studies have shown that Trichoderma is the dominant genus of endophytic fungi in garlic fruit. Endophytic Trichoderma can not only metabolize and produce a variety of substances that promote plant growth hormones, but also enhance the absorption of mineral elements by plants by increasing the solubility of slightly soluble or insoluble substances in the soil. In addition, endophytic Trichoderma can significantly promote seed germination, root growth and yield increase of plants, and improve the host's tolerance to environmental stress. Previous studies have shown that the height, number of leaves and biomass of garlic fruit seedlings inoculated with endophytic Trichoderma increased significantly, and the chlorophyll content and antioxidant enzyme activity increased significantly, which has a good growth-promoting effect on garlic fruit. On this basis, the applicant has conducted research and development of microbial fertilizers to promote the growth of garlic fruit seedlings.

[0004] At present, there are many reports on Trichoderma fertilizers. For example, Chinese patent CN105237146B discloses a preparation method of Trichoderma harzianum fertilizer using sisal residue; Chinese patent CN107236676A discloses a method of preparing dark green Trichoderma fertilizer for Artemisia annua seedlings using spore suspension; Chinese patent CN111004728A discloses a Trichoderma fertilizer prepared by mixing Trichoderma harzianum and Trichoderma koningii in proportion and fermenting with wheat bran, soybean meal, sawdust and urea as raw materials; Chinese patent CN11177737A discloses Trichoderma spinulosa prepared with molasses alcohol concentrate and bean paste as raw materials. The fertilizer efficiency of the above Trichoderma fertilizers has been studied on some vegetables, fruits and cash crops, but for garlic fruit, most of the actual production currently increases the nutrition of the plants by applying compound fertilizers or organic fertilizers, and there is a lack of systematic research on the growth-promoting bacterial fertilizers for garlic fruit. The Trichoderma gemsii used in the present invention is separated from the root system of healthy garlic fruit plants and is the dominant strain of endophytic Trichoderma. Through the early screening test related to the growth-promoting function and the inoculation effect, it was found that the bacterium has a strong ability to produce indoleacetic acid (IAA), and the growth characteristics, biomass and resistance level of the garlic fruit seedlings inoculated with the bacterial solution are significantly higher than those of other multiple strains and the control group. At the same time, the colonization rate of the bacterium in the root system of garlic fruit is 58.87%, which is conducive to better playing a growth-promoting role in the host plant. The selected rice husk powder, wheat bran powder and corn stalks are rich in organic substances such as polysaccharides and cellulose, which can not only provide nutrients for the growth and spore production of Trichoderma gemsii, maintain the stability of the bacterial fertilizer, but also give full play to the utilization value of biological waste resources, thereby achieving the goals of energy saving, environmental protection and soil improvement. Summary of the invention

[0005] The invention aims to provide a special Trichoderma gaimsii fertilizer for garlic fruit seedlings and a preparation method thereof.

[0006] The technical solution adopted by the present invention is as follows.

[0007] A preparation method of a special Trichoderma gaimsii fertilizer for garlic fruit seedlings, the method is as follows:

[0008] (1) Inoculate the Trichoderma genus strain on a potato dextrose agar medium for cultivation, add sterile water after spore production, and scrape the spores on the plate with a sterilized spreader to obtain a bacterial liquid; or inoculate a Trichoderma genus block on a potato dextrose liquid medium and culture it in a constant temperature shaker to obtain a bacterial liquid; filter the obtained bacterial liquid and collect the filtrate to obtain a Trichoderma genus spore suspension, and count the spores on a hemocytometer to obtain a spore suspension of Trichoderma genus. After counting the spores on a hemocytometer, the spore concentration reaches 10 7 ~10 8 Pieces / mL;

[0009] (2) Rice husk powder, wheat bran powder and corn stalk powder were mixed in a mass ratio of 4:4:2 to obtain a mixture, and 0.1-0.2% of magnesium sulfate, 0.3-0.4% of calcium phosphate and 0.1-0.2% of potassium dihydrogen phosphate were added to the mixture. The mixture was placed in a glass culture bottle, and sterile water was added and stirred evenly. The mixture was then sterilized in an autoclave. After the culture bottle was cooled, 1% of the mass of the mixture was added to each bottle. The mixture was cultured at 28°C for 5-7 days with a humidity of 60-70%. The spore concentration was detected to be 10 9 ~10 10 / mL, that is, the Trichoderma gemsii seeds are obtained;

[0010] (3) Rice husk powder, wheat bran powder and corn stalk powder were mixed in a mass ratio of 4:4:2 to obtain a mixture, and the mixture was mixed with Trichoderma gemsii seeds in a mass ratio of 90:10. The mixture was fermented in a dark and closed environment at 25-28°C and a humidity of 60-70% for 7-10 days, with the mixture turned over every 3 days. The spore concentration was detected to be 10 9 ~10 10 / mL, and the special Trichoderma gaimsii fertilizer for garlic fruit seedlings is obtained.

[0011] Furthermore, in the above step (1), the Trichoderma genus strain is inoculated on a potato dextrose agar medium and cultured at 25-28°C for 35-40 days. After spores are produced, 4-6 mL of sterile water is added to each dish, and the spores on the plate are scraped with a sterilized coating rod to obtain a bacterial liquid; or 4-6 Trichoderma genus blocks with a diameter of 1 cm are inoculated into 200 mL of potato dextrose liquid culture medium, placed in a constant temperature shaker, and cultured at 180 r / min and 28°C for 4 days to obtain a bacterial liquid; the obtained bacterial liquid is filtered through a sterile funnel with filter paper to collect a Trichoderma genus spore suspension.

[0012] Furthermore, in the above step (2), sterile water twice the volume of the mixture is added to the glass culture bottle, stirred evenly, and then placed in an autoclave for sterilization at 121° C. for 20 minutes.

[0013] Further, the Trichoderma gemsii strain ( Trichoderma gamsii ) was isolated from the roots of healthy garlic fruit in Guangnan County, Wenshan Prefecture, Yunnan Province. The Trichoderma gemsii strain was deposited in the General Microbiology Center of China National Microbiological Culture Collection Administration on December 19, 2022, with the deposit number CGMCC3.23532.

[0014] The Trichoderma gemsii strain of the present invention is cultured on a PDA medium at 25°C for 7 days and the colonies are white and the hyphae are dense. The conidiophores are tree-like, the middle part of the phialopile is slightly swollen, and pairs or multiple clusters appear on a single or terminal branch. The conidia are oval, elliptical or irregularly elliptical, with a size of 2.65-3.88×2.26-2.93μm. ITS , RPB2 and TEF The phylogenetic tree constructed by combining the three fragments showed that the bacteria Trichoderma gamsii The genus Trichoderma was clustered into one branch and identified as Trichoderma gemsii ( Trichoderma gamsii The nucleotide sequence of the Trichoderma gemsii strain is shown in SEQ ID No. 1.

[0015] The invention makes full use of the growth-promoting property of Trichoderma gemsii on garlic fruit seedlings, takes rice husks, wheat bran and corn antagonists as fermentation raw materials, realizes the recycling of plant waste, is environmentally friendly, has low production cost, and has good promotion and application value. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 The Trichoderma gemsii strain of the present invention ( Trichoderma gamsii ) after culturing on PDA medium at 25°C for 7 days.

[0017] Figure 2 This is the fermentation state of the Trichoderma gemsii fertilizer of the present invention. DETAILED DESCRIPTION

[0018] The following is a further description of the present invention in conjunction with the embodiments. The embodiments listed are only some embodiments of the present invention and are not intended to limit the scope of protection of the present invention.

[0019] Example 1

[0020] A preparation method of a special Trichoderma gaimsii fertilizer for garlic fruit seedlings, the method is as follows:

[0021] (1) The Trichoderma gemsii strain was inoculated onto a potato dextrose agar medium and cultured at 25°C for 40 days. After spores were produced, 5 mL of sterile water was added to each dish, and the spores on the plate were scraped off with a sterilized spreading stick to obtain a bacterial solution. The obtained bacterial solution was filtered through a sterile funnel with filter paper and the filtrate was collected to obtain a Trichoderma gemsii spore suspension. After counting with a hemocytometer, the spore concentration reached 10 8 Pieces / mL;

[0022] (2) Rice husk powder, wheat bran powder and corn straw powder were mixed in a mass ratio of 4:4:2 to obtain a mixture, and 0.2% of magnesium sulfate, 0.3% of calcium phosphate and 0.2% of potassium dihydrogen phosphate were added to the mixture. The mixture was placed in a glass culture bottle, and then sterile water twice the mass of the mixture was added and stirred evenly. The mixture was then placed in an autoclave at 121°C for 20 minutes. After the culture bottles were cooled, 1% of the mass of the mixture was added to each bottle. The mixture was cultured at 28°C for 5 days with a humidity of 60%. The spore concentration was detected to be 10 10 / mL, that is, the Trichoderma gemsii seeds are prepared; the mass ratio of rice husk powder, wheat bran powder and corn straw powder must be strictly controlled at 4: 4: 2, and only this ratio can meet the spore concentration requirements;

[0023] (3) Rice husk powder, wheat bran powder and corn straw powder were mixed in a mass ratio of 4:4:2 to obtain a mixture, and the mixture was mixed with Trichoderma gemsii seeds in a mass ratio of 90:10. The mixture was fermented under closed conditions at 28°C and 60% humidity for 7 days, with the mixture turned over every 3 days. The spore concentration was detected to reach 10 10 / mL, that is, the special Trichoderma gaimsii fertilizer for garlic fruit seedlings is prepared.

[0024] Example 2

[0025] A preparation method of a special Trichoderma gaimsii fertilizer for garlic fruit seedlings, the method is as follows:

[0026] (1) The Trichoderma geumii strain was inoculated on a potato dextrose agar medium and cultured at 28°C for 35 days. After spore production, a sterile punch with a diameter of 1 cm was used to take out the bacterial cakes. Five bacterial cakes were inoculated into each bottle of 200 mL potato dextrose liquid medium and placed in a constant temperature shaker at 180 r / min and 28°C for 4 days to obtain a bacterial liquid. After filtering the bacterial liquid, a Trichoderma geumii spore suspension was collected and counted on a hemocytometer until the spore concentration reached 10 7 Pieces / mL;

[0027] (2) Rice husk powder, wheat bran powder and corn straw powder were mixed in a mass ratio of 4:4:2 to obtain a mixture, and 0.1% magnesium sulfate, 0.35% calcium phosphate and 0.1% potassium dihydrogen phosphate were added to the mixture. The mixture was placed in a glass culture bottle, and then sterile water twice the amount of the mixture was added and stirred evenly. The mixture was then placed in an autoclave at 121°C for 20 minutes. After the culture bottles were cooled, 1% of the mass of the mixture was added to each bottle. The mixture was cultured at 28°C for 7 days with a humidity of 70%. The spore concentration was detected to be 10 9 / mL, that is, Trichoderma gemsii seeds are prepared;

[0028] (3) Rice husk powder, wheat bran powder and corn stalk powder were mixed in a mass ratio of 4:4:2 to obtain a mixture. The mixture was mixed with Trichoderma gemsii seeds in a mass ratio of 90:10, and then fermented in a closed dark environment at 28°C and a humidity of 70% for 10 days. The mixture was turned over every 3 days and sterile water was sprayed to maintain the humidity. The spore concentration was detected to reach 10 9 / mL, that is, the special Trichoderma gaimsii fertilizer for garlic fruit seedlings is prepared.

[0029] Example 3

[0030] A preparation method of a special Trichoderma gaimsii fertilizer for garlic fruit seedlings, the method is as follows:

[0031] (1) The Trichoderma geumii strain was inoculated onto a potato dextrose agar medium and cultured at 26°C for 38 days. After spores were produced, 6 mL of sterile water was added to each dish, and the spores on the plate were scraped off with a sterile spreading stick to obtain a bacterial solution. The obtained bacterial solution was filtered through a sterile funnel with filter paper and the filtrate was collected to obtain a Trichoderma geumii spore suspension. After counting with a hemocytometer, the spore concentration reached 10 8 Pieces / mL;

[0032] (2) Rice husk powder, wheat bran powder and corn straw powder were mixed in a mass ratio of 4:4:2 to obtain a mixture, and 0.15% of magnesium sulfate, 0.4% of calcium phosphate and 0.15% of potassium dihydrogen phosphate were added to the mixture. The mixture was placed in a glass culture bottle, and then sterile water twice the mass of the mixture was added and stirred evenly. The mixture was then placed in an autoclave at 121°C for 20 minutes. After the culture bottles were cooled, 1% of the mass of the mixture was added to each bottle. The mixture was cultured at 28°C for 6 days with a humidity of 65%. The spore concentration was detected to be 10 10 / mL, that is, Trichoderma gemsii seeds are prepared;

[0033] (3) Rice husk powder, wheat bran powder and corn straw powder were mixed in a mass ratio of 4:4:2 to obtain a mixture, and the mixture was mixed with Trichoderma gemsii seeds in a mass ratio of 90:10. The mixture was fermented in a closed dark environment at 28°C and a humidity of 65% for 8 days, with the mixture turned over every 3 days. The spore concentration was detected to be 10 10 / mL, that is, the special Trichoderma gaimsii fertilizer for garlic fruit seedlings is prepared.

[0034] The Trichoderma geumii strain described in the above example was isolated from the root system of healthy garlic fruit in Guangnan County, Wenshan Prefecture, Yunnan Province. After culturing on PDA medium at 25°C for 7 days, the colony of the strain was white and the hyphae were dense. The conidiophores were tree-like, with slightly swollen in the middle of the phialopile, appearing in pairs or multiple clusters on a single or terminal branch. The conidia were oval, elliptical or irregularly elliptical, with a size of 2.65–3.88×2.26–2.93μm, see Figure 1 .through ITS , RPB2 and TEF The phylogenetic tree constructed by combining the three fragments showed that the bacteria Trichoderma gamsii The genus Trichoderma was clustered into one branch and identified as Trichoderma gemsii ( Trichoderma gamsii ). The strain has been deposited in the General Microbiological Center of China Microbiological Culture Collection Administration on December 19, 2022, with the deposit number CGMCC3.23532. The reference biological material (strain) is SN-15-1.

[0035] In order to verify the growth-promoting effect of the Trichoderma gametophore fertilizer of the present invention on garlic fruit seedlings, the following experiment was conducted.

[0036] Test Materials:

[0037] The garlic fruit seedlings are uniformly grown and strong, and are two months old.

[0038] Test location:

[0039] Greenhouse of the Arboretum of Southwest Forestry University, Kunming, Yunnan Province.

[0040] Test method:

[0041] The matrix soil prepared by red soil: humus soil: perlite at a mass ratio of 2:2:1 was mixed with the Trichoderma gemsii fertilizer prepared in Example 1 at different ratios, and a total of 4 treatments were set, including 3 test groups and 1 matrix soil control. The specific mass ratios are as follows:

[0042] Treatment 1, substrate soil: Trichoderma gametophore fertilizer = 9:1;

[0043] Treatment 2, substrate soil: Trichoderma gametophore fertilizer = 8:2;

[0044] Treatment 3, substrate soil: Trichoderma gametophore fertilizer = 7:3;

[0045] Treatment 4: Matrix soil.

[0046] The garlic fruit seedlings were planted in plastic nutrient pots of the same specifications according to the above ratio. Each treatment had 15 plants, a total of 60 plants, and watering was done in a timely and quantitative manner according to weather conditions. The plant height and leaf number of garlic fruit seedlings in different treatment groups were measured before planting and 3 months after planting, and the plant height and leaf increment were calculated respectively. The seedlings were taken out and the roots were washed with clean water, and the root length and the fresh weight of the above-ground and underground parts were measured. The above-ground and underground parts of the seedlings were respectively sterilized in a forced air drying oven at 105°C for 30 minutes, and then dried at 65°C to constant weight (accuracy of 0.01g), and the dry weight of the above-ground and underground parts was weighed.

[0047] The test results are shown in Tables 1 and 2.

[0048] Table 1 Growth characteristics of garlic fruit seedlings after treatment with different ratios of Trichoderma gaimsii fertilizer

[0049] Apply bacterial fertilizer Plant height increase (cm) Stem diameter increment (mm) Blade increment (cm) Root length (cm) Apply 10% bacterial fertilizer 5.21±0.14b 1.52±0.15a 1.53±0.13ab 13.27±0.30ab Apply 20% bacterial fertilizer 5.51±0.21ab 1.54±0.12a 1.87±0.13ab 14.07±58a Apply 30% bacterial fertilizer 5.97±0.23a 1.56±0.30a 2.00±0.17a 14.56±0.53a Sterile fertilizer treatment 4.30±0.19c 1.45±0.16a 1.33±0.19b 11.93±0.33b

[0050] Table 2 Biomass of garlic fruit seedlings after treatment with different ratios of Trichoderma genus fertilizer

[0051] Apply bacterial fertilizer Fresh weight of above-ground part (g) Fresh weight of underground part (g) Whole plant fresh weight (g) Dry mass of aboveground part (g) Dry mass of underground part (g) Whole plant dry weight (g) Apply 10% bacterial fertilizer 3.64±0.13bc 4.89±0.16bc 8.53±0.24b 1.52±0.04b 1.64±0.06b 3.16±0.07b Apply 20% bacterial fertilizer 3.87±0.14ab 5.18±0.12ab 9.04±0.19b 1.63±0.04ab 1.79±0.07ab 3.42±0.07b Apply 30% bacterial fertilizer 4.37±0.23a 5.74±0.27a 10.11±0.31a 1.78±0.05a 2.03±0.14a 3.81±0.18a Sterile fertilizer treatment 3.11±0.14c 4.31±0.15c 7.41±0.21c 1.28±0.06c 1.47±0.10b 2.69±0.05c

[0052] The bacterial fertilizers listed in Tables 1 and 2 are Trichoderma gemsii bacterial fertilizers. The data in Tables 1 and 2 represent mean ± standard error, and different lowercase letters represent significant differences among different treatments (P<0.05).

[0053] The test results show that after applying different ratios of Trichoderma gaimsii fertilizer, the plant height increment, the fresh weight of the whole plant, the dry weight of the aboveground part and the dry weight of the whole plant are significantly higher than those of the control group treated with sterile fertilizer, indicating that the Trichoderma gaimsii fertilizer of the present invention has a growth-promoting effect on garlic fruit seedlings, and the growth-promoting effect is continuously enhanced with the increase of the amount of fertilizer applied. After applying 30% of the bacterial fertilizer, the garlic fruit seedlings, except for the increase in stem thickness, all other indicators are significantly higher than the control, followed by the treatment of applying 20% ​​of the bacterial fertilizer, so it is recommended that the amount of bacterial fertilizer used is 20% to 30% of the total amount of the substrate.

[0054] Sequence Listing

[0055] <110> Southwest Forestry University

[0056] <120> Preparation method of Trichoderma gaimsii fertilizer specially used for garlic fruit seedlings

[0057] <130>

[0058] <160> 1

[0059] <170> SIPOSequenceListing 1.0

[0060] <210> 1

[0061] <211> 598

[0062] <212> DNA

[0063] <213> Trichoderma gamsii

[0064] <400> 1

[0065] gtacaaggtc tccgttggtg aaccagcgga gggatcatta ccgagtttac aactcccaaa 60

[0066] cccaatgtga accataccaa actgttgcct cggcggggtc acgccccggg tgcgtcgcag 120

[0067] ccccggaacc aggcgcccgc cggagggacc aaccaaactc ttttctgtag tcccctcgcg 180

[0068] gacgttattt cttacagctc tgagcaaaaa ttcaaaatga atcaaaactt tcaacaacgg 240

[0069] atctcttggt tctggcatcg atgaagaacg cagcgaaatg cgataagtaa tgtgaattgc 300

[0070] agaattcagt gaatcatcga atctttgaac gcacattgcg cccgccagta ttctggcggg 360

[0071] catgcctgtc cgagcgtcat ttcaaccctc gaacccctcc ggggggtcgg cgttggggat 420

[0072] cgggaacccc taagacggga tcccggcccc gaaatacagt ggcggtctcg ccgcagcctc 480

[0073] tcctgcgcag tagtttgcac aactcgcacc gggagcgcgg cgcgtccacg tccgtaaaac 540

[0074] acccaacttc tgaaatgttg acctcggatc aggtaggaat acccgctgaa cttaagat 598

Claims

1. An application of Trichoderma gamesis fertilizer in promoting the growth of garlic fruit seedlings, characterized in that: The preparation method of described Games Trichoderma fertilizer is as follows: (1) Inoculate the Trichoderma genus strain on a potato dextrose agar medium for cultivation, add sterile water after spore production, and scrape the spores on the plate with a sterilized spreader to obtain a bacterial liquid; or inoculate a Trichoderma genus block on a potato dextrose liquid medium and culture it in a constant temperature shaker to obtain a bacterial liquid; filter the obtained bacterial liquid and collect the filtrate to obtain a Trichoderma genus spore suspension, and count the spores on a hemocytometer to obtain a spore suspension of Trichoderma genus. After counting the spores on a hemocytometer, the spore concentration reaches 10 7 ~10 8 Pieces / mL; (2) Rice husk powder, wheat bran powder and corn stalk powder were mixed in a mass ratio of 4:4:2 to obtain a mixture, and 0.1-0.2% of magnesium sulfate, 0.3-0.4% of calcium phosphate and 0.1-0.2% of potassium dihydrogen phosphate were added to the mixture. The mixture was placed in a glass culture bottle, and sterile water was added and stirred evenly. The mixture was then sterilized in an autoclave. After the culture bottle was cooled, 1% of the mass of the mixture was added to each bottle. The mixture was cultured at 28°C for 5-7 days with a humidity of 60-70%. The spore concentration was detected to be 10 9 ~10 10 / mL, that is, the Trichoderma gemsii seeds are obtained; (3) Rice husk powder, wheat bran powder and corn stalk powder were mixed in a mass ratio of 4:4:2 to obtain a mixture, and the mixture was mixed with Trichoderma gemsii seeds in a mass ratio of 90:

10. The mixture was fermented in a dark and closed environment at 25-28°C and a humidity of 60-70% for 7-10 days, with the mixture turned over every 3 days. The spore concentration was detected to be 10 9 ~10 10 / mL, that is, the special Trichoderma gaimsii fertilizer for garlic fruit seedlings is obtained; The Trichoderma gamesis fertilizer is added into the substrate soil for cultivating garlic fruit seedlings and mixed.

2. The application of Trichoderma gaimsii fertilizer in promoting the growth of garlic fruit seedlings as claimed in claim 1, characterized in that: In the step (1), the Trichoderma genus strain is inoculated on a potato dextrose agar medium and cultured at 25-28°C for 35-40 days. After spores are produced, 4-6 mL of sterile water is added to each dish, and spores on the plate are scraped with a sterilized coating rod to obtain a bacterial liquid; or 4-6 Trichoderma genus blocks with a diameter of 1 cm are inoculated into 200 mL of potato dextrose liquid medium, placed in a constant temperature shaker, and cultured at 180 r / min and 28°C for 4 days to obtain a bacterial liquid; the obtained bacterial liquid is filtered through a sterile funnel with filter paper to collect a Trichoderma genus spore suspension.

3. The application of Trichoderma gaimsii fertilizer in promoting the growth of garlic fruit seedlings as claimed in claim 1, characterized in that: In the step (2), add sterile water twice the amount of the mixture into the glass culture bottle, stir evenly, and then place in an autoclave for sterilization at 121° C. for 20 minutes.

4. The application of Trichoderma gaimsii fertilizer in promoting the growth of garlic fruit seedlings as claimed in claim 1, 2 or 3, characterized in that: The Trichoderma gemsii strain ( Trichoderma gamsii ) was isolated from the roots of healthy garlic fruit in Guangnan County, Wenshan Prefecture, Yunnan Province. The Trichoderma gemsii strain was deposited in the General Microbiology Center of China National Microbiological Culture Collection Administration on December 19, 2022, with the deposit number CGMCC3.23532.

Images