A method for detecting the compatibility of an injection solution with a packaging material and a microfluidic detection system

Abstract

The application provides a kind of injection and package material compatibility detection method and microfluidic detection system, including sheet microfluidic sheet, electrode module, cover plate, sheet microfluidic sheet and electrode module are sealed, form chamber;Sheet microfluidic sheet includes several test partitions, several sheet magnets, several test partitions are symmetrically distributed, each test partition includes several test units, liquid inlet channel and liquid addition tank;Several test units are communicated with liquid addition tank by liquid inlet channel;Sheet magnet is symmetrically distributed in the non-test area of sheet microfluidic sheet, and each sheet microfluidic sheet is spliced in Z axis direction by sheet magnet through varying magnetic force;Sheet microfluidic sheet is spliced in Z axis direction by sheet magnet to form the chamber of liquid addition tank and several test units;Electrode module includes test electrode, test electrode extends into the chamber of sheet microfluidic sheet, liquid addition tank, and cover plate can cover and abut liquid addition tank.The application realizes high-throughput detection, easy to disassemble and remove waste liquid, and is suitable for a variety of analytical chemistry detection methods.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of analytical detection technology, in particular to a method for detecting the compatibility of injection liquid and packaging materials and a microfluidic detection system. BACKGROUND

[0002] The packaging materials for medical injection devices mainly include medicinal butyl, polyethylene, polypropylene, polyethylene terephthalate, etc. Bisphenol A can be added in the processing and manufacturing process of plastic products to make them have the characteristics of colorless and transparent, durable, light and impact resistance, etc., which can prevent acidic substances from corroding the outer packaging from the inside. Bisphenol A (BPA) has weak estrogenic activity and strong anti-androgenic activity, which can directly or through its derivatives interfere with the normal endocrine function of organisms. At the same time, antioxidant 2,6-di-tert-butyl-4-methylphenol BHT is one of the volatile components known to exist in the injection rubber plug of medical devices. Studies have shown that BHT has different degrees of toxic effects on the liver, spleen and stomach of the human body. The migration amount of BHT in the rubber plug to the drug solution plays an important role in the compatibility study of the rubber plug and the drug.

[0003] Microfluidic chip, also known as Lab-on-a-chip, is a micro-laboratory that relies on surface tension, fluid resistance, energy dissipation, etc. to control the flow direction of fluid and shorten the reaction time. Microfluidic chip integrates sample pretreatment, separation and detection processes in the fields of analytical chemistry, materials science, electronic science, mechanical science, molecular biology and medicine into a small chip, which is beneficial to realize the automation, integration, miniaturization, low consumption, high efficiency and portability of sample pretreatment and detection analysis. Compared with traditional methods, microfluidic technology realizes the demand for on-site and real-time detection of bisphenol A and BHT, and has the advantages of miniaturization, high throughput, rapidity, integration and low consumption. SUMMARY

[0004] In order to realize high-throughput detection, miniaturization and strong operability of the compatibility detection system, the present application provides a microfluidic detection system for detecting the compatibility of injection liquid and packaging materials, a preparation method thereof and a method for detecting the compatibility of injection liquid and packaging materials.

[0005] In a first aspect, the present application provides a microfluidic detection system, comprising a sheet-type microfluidic chip, an electrode module and a cover plate, wherein the sheet-type microfluidic chip is bonded and sealed with the electrode module to form a chamber, and the sheet-type microfluidic chip abuts against the cover plate.

[0006] The chip-type microfluidic chip comprises components: a plurality of test partitions, a plurality of chip-type magnets, the plurality of test partitions are symmetrically distributed, each of the plurality of test partitions comprises a plurality of test units, a plurality of partition magnets, a liquid inlet channel and a liquid addition tank; the plurality of test units are communicated with the liquid addition tank through the liquid inlet channel.

[0007] Preferably, the sidewall of the liquid inlet channel is provided with a plurality of pole pieces, and the plurality of pole pieces correspond to each other one by one.

[0008] Preferably, the plurality of pole pieces are capacitance test pole pieces, and the micro changes of capacitance can be measured to monitor the shunt process of charged droplets.

[0009] The plurality of chip-type magnets are symmetrically distributed in the non-test area of the chip-type microfluidic chip, the plurality of partition magnets are symmetrically distributed in the non-test area and the non-channel area of the plurality of test units, and the plurality of chip-type magnets are spliced in the Z-axis direction by changing the magnetic force of each chip-type microfluidic chip.

[0010] Further, the plurality of test units are communicated with the test partition liquid tank through the liquid inlet channel, and the test partition liquid tank is communicated with the liquid addition tank.

[0011] Further, the plurality of test units comprise a plurality of test unit reaction groups, a liquid inlet channel corresponding to each of the plurality of test unit reaction groups, a partition magnet and a unit magnet, and the plurality of test unit reaction groups are communicated with the test partition liquid tank or the liquid addition tank through the liquid inlet channel.

[0012] The plurality of test unit reaction groups comprise a plurality of cavities, and the plurality of cavities are communicated with the test partition liquid tank or the liquid addition tank through the liquid inlet channel.

[0013] Preferably, the cavities between the reaction groups of the plurality of test unit reaction groups are communicated one by one through the liquid inlet channel.

[0014] The partition magnets and the unit magnets are symmetrically distributed in the non-test area and the non-channel area of the plurality of test units, and the partition magnets and the unit magnets are spliced in the Z-axis direction by changing the magnetic force of each of the plurality of test units.

[0015] Further, the electrode module comprises a base and a test electrode, the base is connected to the test electrode, and the test electrode extends into the cavities, the test partition liquid tank and the liquid addition tank formed by the chip-type microfluidic chip.

[0016] Preferably, the chip-type microfluidic chip is spliced in the Z-axis direction, and the electrode module is bonded to the outermost two layers in the Z-axis direction to seal the chip-type microfluidic chip.

[0017] Preferably, the microfluidic chip is spliced in the Z-axis direction, and the electrode module is attached to the outermost layer in the Z-axis direction, and the other side is attached to the optical lens module.

[0018] Further, the cover plate comprises a component rotation knob, a connecting arm, and a baffle, the rotation knob can adjust the circumferential movement of the baffle through the connecting arm, and the baffle can cover and abut the liquid adding groove.

[0019] A microfluidic detection system is provided, which is composed of a spliced microfluidic chip, an MCU, a power supply, and a storage element. The power supply turns on the MCU, which controls the voltage and polarity parameters of the test electrodes of the microfluidic chip, controls the flow rate, droplet size, droplet flow direction, and reaction duration of the liquid.

[0020] In a second aspect, the application provides a preparation method of a microfluidic detection system for detecting the compatibility of an injection solution and a packaging material.

[0021] S100: Preparation of electrode module and substrate:

[0022] 1) A substrate is provided, and through holes and through grooves are opened, copper is deposited and electroplated to form a metal plating layer, the substrate and the metal plating layer are wrapped with photosensitive glue, exposed, developed, and the photosensitive glue in the non-pattern area is removed, the through holes are filled with electroplating deep plating, the plate is ground and flattened, the upper metal layer is removed, and the metal columns and the electrode pieces are exposed, forming a first liquid inlet channel, a second liquid inlet channel, and a third liquid inlet channel, the electrode pieces are distributed on the side walls of the first liquid inlet channel, the second liquid inlet channel, and the third liquid inlet channel, and the third liquid inlet channel is connected to the first liquid inlet channel and the second liquid inlet channel;

[0023] 2) The temperature is raised above the glass transition temperature of the photosensitive glue, so that the photosensitive glue is in a semi-solid state, and the photosensitive glue is etched with chemical medicine water to remove the photosensitive glue and peel off the substrate and the electrode module;

[0024] S200: Preparation of substrate stack:

[0025] Through holes are opened in the substrate formed by S100, and unit magnets are embedded inside, the unit magnets press the substrates formed by S100 into a substrate stack with a cavity, external force can peel off the substrates layer by layer, the thickness of the substrate stack is controlled, and the reaction waste liquid is easily removed;

[0026] S300: Preparation of test electrode module:

[0027] Flash etching is performed on the exposed metal columns of the electrode module to form trapezoidal test electrodes, through holes can be etched in the test electrodes to place optical devices (such as optical fiber probe heads), and the test electrodes are connected to the base;

[0028] S400: Similarly, the substrate stack prepared in step S300 is pressure bonded with the test electrode prepared in step S300, so that the test electrode extends into the chamber of the substrate stack, and the test electrode or lens can be pressure bonded on the other side of the substrate stack, thereby forming a test unit;

[0029] S500: Similarly, two left-right symmetrical test units are prepared, and the first liquid inlet channel of the test unit is connected to the test subarea first subliquid groove of the test subarea. The test subarea first subliquid groove contains a chamber, and the side edge is a circular metal coating. The test electrode can extend into the chamber of the test subarea first subliquid groove;

[0030] S600: Two up-down symmetrical test units are prepared, and the test subarea first subliquid groove and the test subarea second subliquid groove are connected to the subarea liquid inlet channel of the liquid inlet groove. The liquid inlet groove transports solution to the test subarea first subliquid groove and the test subarea second subliquid groove through the subarea liquid inlet channel. The liquid inlet groove contains a chamber, and the side edge is a circular metal coating. The test electrode can extend into the chamber of the liquid inlet groove;

[0031] S700: The test subarea embedded subarea magnet is prepared, and the subarea magnet realizes the test subarea pressure bonding in a sheet form, which can be disassembled layer by layer under external force;

[0032] The up-down-left-right symmetrical design of four test subareas prepares a sheet microfluidic sheet, and the sheet microfluidic sheet is embedded with a sheet magnet. The sheet magnet realizes the sheet microfluidic sheet pressure bonding in a sheet form, which can be disassembled layer by layer under external force;

[0033] S800: The sheet microfluidic sheet is assembled layer by layer by the magnetic force of the sheet magnet, the subarea magnet, and the unit magnet, the test electrode or the lens is pressure bonded, and the microfluidic chip is formed.

[0034] A cover plate is provided, a rotating knob is arranged, a connecting arm is arranged, and a blocking piece is arranged. The blocking piece can block the liquid inlet groove of the sealed sheet microfluidic sheet. The blocking piece is connected to the rotating knob through the connecting arm. By rotating the rotating knob, the liquid inlet groove can be sealed and opened.

[0035] S900: The microfluidic detection system is composed of the microfluidic chip prepared in S100-S800, MCU, power supply, and storage element. The power supply is turned on to control the voltage, electrode polarity, and other parameters of the test electrode of the microfluidic chip by the MCU. The flow rate, droplet size, droplet flow direction, and reaction time of the liquid inlet are controlled.

[0036] The third aspect is a method for detecting the compatibility of an injection solution and a packaging material. The microfluidic detection system is provided. Different polarities and sizes of voltage are applied to adjacent test electrodes to drive charged droplets to branch and react. At least one of surface-enhanced Raman spectroscopy and electrochemical sensor method is used to quantitatively detect bisphenol A and BHT in the injection solution.

[0037] The present application has the following beneficial effects:

[0038] 1) By setting by partition, grouping and several test units, the liquid droplet is scaled from large scale to micro trace, micro trace detection is realized, and then multiple control groups are tested in parallel, liquid is added once, multiple parallel control tests are realized, and high-efficiency high-throughput detection is realized;

[0039] 2) By setting sheet magnets, partition magnets and unit magnets, the sheet microfluidic chip can be freely assembled and disassembled by power change, which is convenient for adjusting the thickness of the microfluidic chip according to the contact charged liquid droplet of different scales, and then controlling the cross-sectional area of the channel, and it is also convenient for disassembling and removing waste liquid after reaction;

[0040] 3) By forming an electrode module with a similar size as the sheet microfluidic chip, the electrodes of the electrode module can accurately extend into the chamber formed after the stacking of the sheet microfluidic chip, avoiding the electrodes touching the side wall of the chamber and causing short circuit;

[0041] 4) By stacking the sheet microfluidic chip and the electrode module, two electrode modules can be bonded for electrochemical sensor testing, and one electrode module can be bonded with an optical lens on the other side for surface enhanced Raman and fluorescence testing;

[0042] 5) The polarity and voltage of the electrode can drive the contact charged liquid droplet to move in the channel, adjust the size of the charged liquid droplet, and detect the capacitance change in the channel in real time. BRIEF DESCRIPTION OF DRAWINGS

[0043] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed in the embodiments. Obviously, the drawings described below are only some embodiments of the present application, and other drawings can also be obtained by those skilled in the art without creative labor.

[0044] Figure 1 It is a schematic diagram of the metal filling hole structure of the through hole of the substrate;

[0045] Figure 2 It is a schematic diagram of the separation of the electrode plating layer and the laminated substrate;

[0046] Figure 3 It is a schematic diagram of the electrode formed by the electrode plating layer;

[0047] Figure 4 It is a side sectional view of a microfluidic system test device;

[0048] Figure 5 It is a side sectional view of another test device of a microfluidic system;

[0049] Figure 6 A test unit cross-sectional view of the microfluidic system test device;

[0050] Figure 7 A test partition cross-sectional view of the microfluidic system test device;

[0051] Figure 8 A test partition partitioning groove cross-sectional view of the microfluidic system test device;

[0052] Figure 9 A test partition partitioning groove cross-sectional view of the microfluidic system test device;

[0053] Figure 10 A test unit cross-sectional view of the microfluidic system test device;

[0054] Figure 11 A test unit cross-sectional view of the microfluidic system test device;

[0055] Figure: substrate 1, photosensitive adhesive 2, metal plating layer 3, pole piece 3a, pole piece 3b, electrode substrate 4, metal column 5, base 6, test electrode 7, electrode module 71, electrode module 72, lens 8, chamber 9, test unit 10, first test unit reaction group 101, second test unit reaction group 102, first liquid inlet channel 103a, second liquid inlet channel 103b, third liquid inlet channel 103c, unit magnet 104, test partition 20, test partition first partitioning groove 201, test partition second partitioning groove 202, partition liquid inlet channel 203, partition magnet 204a, partition magnet 204b, sheet microfluidic sheet 30, liquid addition groove 301, sheet magnet 304a, sheet magnet 304b, cover plate 40, rotating knob 401, connecting arm 402, baffle 403. DETAILED DESCRIPTION

[0056] In order to enable a clearer understanding of the above-mentioned purposes, features and advantages of the present application, the present application will be further described below in conjunction with the accompanying drawings and examples. It should be noted that the examples of the present application and the features in the examples can be combined with each other without conflict.

[0057] The present application will be further described below in conjunction with the accompanying drawings and specific examples.

[0058] Example 1:

[0059] As Figures 1-5 , the present application provides a preparation method of a microfluidic detection system:

[0060] S100: preparation of electrode module 7 and substrate:

[0061] 1) Provide a substrate 1, open a number of through holes and through slots, copper electroplating to form a metal plating layer 3, fill the hole with photosensitive glue 2, fill the slot with photosensitive glue 2, wrap the substrate 1 and the metal plating layer 3, expose and develop, remove the photosensitive glue in the non-pattern area, fill the hole with electroplating, grind the plate flat, remove the upper metal layer, expose the metal column 5 and the first liquid inlet channel 103a, the second liquid inlet channel 103b, the third liquid inlet channel 103c of the pole piece 3b, the third liquid inlet channel 103c communicates with the second liquid inlet channel 103b and the third liquid inlet channel 103c.

[0062] 2) Raise the temperature above the glass transition temperature of the photosensitive glue 2, so that the photosensitive glue 2 is in a semi-cured state, etch the photosensitive glue 2 with chemical water, remove the photosensitive glue 2, and peel off to form a substrate and electrode module 7.

[0063] S200: Preparation of substrate superstructure:

[0064] Open through holes on the substrate formed by S100, embed cell magnets 104, and press a number of substrates formed by S100 into a superstructure with a chamber 9. The external force can peel off the substrate layer by layer, control the thickness of the superstructure, and facilitate the removal of reaction waste liquid.

[0065] S300: Preparation of test electrode 7:

[0066] Side etching of the metal column 5 exposed on the electrode module 7 forms a trapezoidal electrode, and the electrode is connected to the base 6.

[0067] S400: Similarly, the substrate superstructure prepared in step S200 and the test electrode 7 prepared in step S300 are pressed and fixed with photosensitive glue 2, so that the test electrode 7 extends into the chamber 9 of the substrate superstructure, and the test electrode 7 or lens 8 can be pressed on the other side of the substrate superstructure, thereby forming a test unit 10.

[0068] S500: As Figures 6-9 Similarly, two left and right symmetrical test units 10 are prepared, and the first liquid inlet channel 103a and the second liquid inlet channel 103b of the test unit 10 communicate with the test partition first liquid groove 201 of the test partition 20. The test partition first liquid groove 201 contains the chamber 9, and the side is a circular metal plating layer 3. The test electrode 7 can extend into the chamber 9 of the test partition first liquid groove 201.

[0069] S600: Two up and down symmetrical test units 10 are prepared, the test partition first liquid groove 201, the test partition second liquid groove 202 and the partition liquid inlet channel 203 of the liquid addition groove 301 are communicated, and the liquid addition groove 301 transports solution to the test partition first liquid groove 201 and the test partition second liquid groove 202 through the partition liquid inlet channel 203. The liquid addition groove 301 contains the chamber 9, and the side is a circular metal plating layer 3. The test electrode 7 can extend into the chamber 9 of the liquid addition groove 301.

[0070] S700: The embedded partition magnet 204a and the embedded partition magnet 204b in the test partition 20 can be sheet-type pressure-welded and can be layer by layer disassembled under external force.

[0071] The sheet-type microfluidic chip 30 is made by designing four test partitions 20 symmetrically up and down and left and right, and the embedded sheet magnet 304a and the embedded sheet magnet 304b in the sheet-type microfluidic chip 30 can be sheet-type pressure-welded and can be layer by layer disassembled under external force.

[0072] S800: As shown in FIG. 8, the sheet-type microfluidic chip 30 is formed by layer by layer buckling and splicing of the sheet magnet 304a, the sheet magnet 304b, the partition magnet 204a, the partition magnet 204b, and the unit magnet 104, and pressure-welding of the test electrode 7 or the lens 8. Figure 10

[0073] A cover plate 40 is provided, a rotating knob 401 is arranged, a connecting arm 402 is arranged, and a blocking piece 403 is arranged. The blocking piece 403 can block the liquid adding groove 301 of the sealed sheet-type microfluidic chip 30. The blocking piece 403 is in communication with the rotating knob 401 through the connecting arm 402. The sealing and opening of the liquid adding groove 301 can be realized by rotating the rotating knob 401.

[0074] S900: The microfluidic detection system is formed by the microfluidic chip made as above, an MCU, a power supply, and a storage element. The power supply is turned on to control the MCU. The voltage, electrode polarity, and other parameters of the test electrode 7 of the microfluidic chip are controlled by the MCU. The flow rate, droplet size, droplet flow direction, and reaction time of the liquid are controlled.

[0075] Example 2: Quantitative detection of bisphenol A and BHT by electrochemical sensor

[0076] 1) S100: The electrode module 7 is prepared according to the method of Example 1. The test electrode is selectively gold-plated (only the electrode 7 in the test unit such as the first test unit reaction group 101 and the second test unit reaction group 102 is gold-plated. The test electrode 7 in the liquid adding groove 301 and the test electrode 7 in the test partition first liquid separation groove 201 are not gold-plated). The nanometer gold layer with a thickness of 2 um is evaporated and sputtered. The microfluidic detection system is prepared according to steps S100-S900.

[0077] 2) The test electrode 7 in the liquid adding groove 301 and the test electrode 7 in the test partition first liquid separation groove 201 are electrified to form electrodes with opposite polarity. The mixed solution of graphene oxide and HAuCl4 that has been electrified by contact is added dropwise into the liquid adding groove 301. The test electrode 7 strengthens the polarization of the surface particles of the droplet.

[0078] ​3) microfluidic detection system does not give the second test unit reaction group 102 in the test unit 10 voltage, the test partition first liquid tank 201, the first test unit reaction group 101 voltage, when the test electrode 7 in the liquid tank 301, the test electrode 7 of the test partition first liquid tank 201 is electrified to form the voltage of opposite polarity, the voltage of the liquid tank 301 is closed, the droplet flows to the test partition first liquid tank 201, then the test partition first liquid tank 201, the first test unit reaction group 101 is applied to the voltage of opposite polarity, the droplet flows to the first test unit reaction group 101, and is uniformly distributed in the chamber 9;

[0079] 4) the reduction potential is such that graphene oxide and HAuCl4 are directly and simultaneously electrochemically reduced on the electrode surface, resulting in a low-cost and environmentally friendly nanocomposite electrochemical sensor based on gold nanoparticles / electrochemically reduced graphene oxide;

[0080] 5) the excess graphene oxide and HAuCl4 mixed solution is sucked away by a peristaltic pump negative pressure, the contact charged sample (containing bisphenol A, BHT) is added dropwise, the voltage polarity inside the adjacent chamber 9 is adjusted to drive the sample to flow to the chamber 9 of the first test unit reaction group 101, and the content of the sample bisphenol A, BHT is quantitatively determined by cyclic voltammetry and impedance spectroscopy. Similarly, multiple partition experiments can be performed to form a parallel control group;

[0081] 6) stop electrifying, peel off the sheet microfluidic 30 under external force, remove the reaction waste liquid, clean the sheet microfluidic 30, and reassemble.

[0082] Example 3: quantitative detection of bisphenol A and BHT by surface enhanced Raman spectroscopy

[0083] 1) take S100 prepared in example 1: prepare electrode module 7, press together with lens 8 in substrate stack, electrode of electrode module 7 extends into chamber 9, corresponding to lens 8, lens 8 is the window for micro Raman spectrometer to collect spectrum.

[0084] 2) Same as example 2, by forming opposite polarity voltage between adjacent electrodes, drive the charged droplets to the first test unit reaction group 101 and the second test unit reaction group 102. In this example, mix sodium chloride solution and gold sol enhanced substrate to form SERS substrate, after contact charging, by polarity driving, the mixture of sodium chloride solution and gold sol enhanced substrate is uniformly dispersed in the first test unit reaction group 101, after charging the solution of bisphenol A or BHT to be tested, by polarity driving, the solution is uniformly dispersed in the second test unit reaction group 102, turn off the electrode 7 of the first test unit reaction group 101, enhance the voltage of the second test unit reaction group 102, drive the mixture of sodium chloride solution and gold sol enhanced substrate to the second test unit reaction group 102, after uniform mixing of the two solutions, measure and collect Raman spectrum, and quantitatively analyze;

[0085] 3) By peristaltic pump negative pressure, suck away the excess mixture. Similarly, multiple partition experiments can be performed to form parallel control groups.

[0086] 4) Stop energizing, peel off the sheet microfluidic 30 by external force, remove the reaction waste liquid, clean the sheet microfluidic 30, and reassemble.

[0087] The microfluidic detection system provided in this example can realize high-throughput detection, easy disassembly and waste removal, and can be suitable for various analytical chemistry detection methods, such as electrochemical sensors, surface enhanced Raman and fluorescence analysis.

[0088] From the above specific embodiments, those skilled in the art can easily implement the present application. However, it should be understood that the present application is not limited to the above specific embodiments. Based on the disclosed embodiments, those skilled in the art can arbitrarily combine different technical features to realize different technical solutions.

Claims

1. A microfluidic detection system, characterized in that: It includes a sheet-type microfluidic sheet, an electrode module, and a cover plate. The sheet-type microfluidic sheet is bonded and sealed to the electrode module to form a cavity, and the sheet-type microfluidic sheet abuts against the cover plate. The microfluidic sheet includes components: several test zones and several sheet magnets. The several test zones are symmetrically distributed, and each of the several test zones includes several test units, a liquid inlet channel and a liquid addition tank. The plurality of test units are connected to the liquid filling tank through the liquid inlet channel; The plurality of plate magnets are symmetrically distributed in the non-test area of ​​the plate microfluidic sheet, and the plurality of plate magnets splice each of the plate microfluidic sheets in the Z-axis direction by varying magnetic force; The microfluidic sheet is assembled in the Z-axis direction by several sheet magnets to form the liquid filling tank and the chamber of several test units; The electrode module includes a test electrode that extends into the chamber and the liquid filling tank formed by splicing the sheet-type microfluidic sheets in the Z-axis direction. The cover plate can cover and abut against the liquid filling tank; The plurality of test units include a plurality of test unit reaction groups and a liquid inlet channel corresponding to each of the plurality of test unit reaction groups. The plurality of test unit reaction groups are connected to the liquid addition tank through the liquid inlet channel. The reaction group of the plurality of test units contains a plurality of chambers, and the plurality of chambers are connected to the liquid addition tank through the liquid inlet channel; The plurality of chambers in the plurality of test unit reaction groups can be connected one by one through the liquid inlet channel; The plurality of test units are connected to the test subdivision liquid tank through the liquid inlet channel, and the test subdivision liquid tank is connected to the liquid inlet channel of the liquid addition tank and the reaction group of the plurality of test units; The test subdivision liquid tank is connected to several chambers of the reaction group of the several test units; The test electrode extends into the test dividing liquid tank and several chambers; Each of the plurality of test partitions includes a plurality of partition magnets, which are symmetrically distributed in the non-test area and non-channel area of ​​the plurality of test units. The plurality of partition magnets splice each of the plurality of test partitions in the Z-axis direction by varying magnetic force. The plurality of test units include a plurality of unit magnets, which are symmetrically distributed in the non-test area and non-channel area of ​​the plurality of test units, and the unit magnets are spliced ​​together in the Z-axis direction by varying magnetic force. A sheet-like microfluidic sheet is spliced ​​together in the Z-axis direction, and the electrode module is bonded to the outermost two layers in the Z-axis direction to seal the sheet-like microfluidic sheet; a sheet-like microfluidic sheet is spliced ​​together in the Z-axis direction, and the electrode module is bonded to the outermost layer in the Z-axis direction, with an optical lens module bonded to the other side.

2. The microfluidic detection system as described in claim 1, characterized in that: The sidewall of the liquid inlet channel is provided with several electrodes, which are opposite to each other in a one-to-one correspondence.

3. The microfluidic detection system as described in claim 1, characterized in that: The cover plate includes a rotary knob, a connecting arm, and a baffle. The rotary knob can adjust the circumferential movement of the baffle through the connecting arm, and the baffle can cover and abut against the liquid filling tank.

4. The microfluidic detection system as described in claim 1, characterized in that: The system provides a microfluidic chip formed by splicing the microfluidic chips on the Z-axis, an MCU, and a power supply. When the power supply is turned on, the MCU controls the voltage and polarity parameters of the test electrodes of the microfluidic chip, and controls the flow rate, droplet size, droplet direction and reaction time of the liquid.

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