Image acquisition device and adjustment method, immunoblot detection system, and storage medium

Abstract

The application provides an image acquisition device and a regulating method, an immunoblotting analysis system and a readable storage medium. The image acquisition device comprises a plurality of light source assemblies for lighting and a control mechanism for executing the regulating method. The regulating method comprises the following steps: obtaining a first image of a target area on a sample plate; obtaining relevant gray values of the first image; and adjusting the current provided to each light source assembly according to the relevant gray values of the first image. The image acquisition device can automatically adjust the intensity of the light provided by the light source mechanism under the control of the control mechanism, achieve the purpose of uniformly lighting the object of the image to be acquired, improve the quality of the acquired image, and when applied to an immunoblotting detection system, can improve the reliability of the analysis result.

Description

Technical Field

[0001] This invention belongs to the field of immunoblotting detection technology, specifically relating to an image acquisition device and adjustment method, an immunoblotting detection system and a storage medium. Background Technology

[0002] Immunoblotting, also known as protein blotting, is a method for detecting a specific protein in a complex sample based on the specific binding of antigens and antibodies. Due to its advantages such as large analytical capacity, high sensitivity, and strong specificity, it has become one of the most commonly used methods for detecting protein characteristics, expression, and distribution.

[0003] In the medical field, both manual and automated immunoblotting are widely used. Manual testing can only provide qualitative analysis, and the results are subject to subjectivity, low efficiency, and susceptibility to errors. Existing automated testing technologies mostly output qualitative or semi-quantitative results with low accuracy. In practical applications, doctors often find it difficult to provide more reasonable, effective, and precise treatment plans based on qualitative or semi-quantitative results.

[0004] To address the aforementioned issues, several methods have emerged that quantify immunoblotting analysis results by analyzing the grayscale values ​​of images of immunoblotting membrane strips. These methods rely on image acquisition devices with high performance, capable of providing uniform illumination and obtaining clear images. However, existing image acquisition devices generally suffer from poor light source uniformity and cumbersome adjustment processes. Summary of the Invention

[0005] The purpose of this invention is to provide an image acquisition device and adjustment method, an immunoblotting detection and analysis system, and a readable storage medium, with the aim of obtaining clear images of immunoblotting detection membranes and improving the accuracy of immunoblotting detection and analysis.

[0006] To achieve the above objectives, the present invention provides an adjustment method for an image acquisition device, the image acquisition device comprising multiple light source components for illumination; comprising the following steps:

[0007] Acquire the first image of the target area on the carrier plate;

[0008] Obtain the relevant grayscale values ​​of the first image; and,

[0009] The current supplied to each of the light source components is adjusted according to the relevant grayscale value of the first image.

[0010] Optionally, the relevant grayscale values ​​include the average grayscale value of the first image and the average grayscale value of the image region in the first image corresponding to each of the light source components.

[0011] Optionally, the number of light source components is n. 2 1, and n 2 The light source components are arranged in an n×n array; each light source component includes m light sources, and all the light sources are arranged in an n×(n×m) array, where n is an odd number greater than 1 and m is an integer greater than 1;

[0012] The step of obtaining the relevant grayscale value of the first image includes:

[0013] Obtain the average grayscale value of the image region corresponding to each of the light source components in the first image;

[0014] The average gray value of the first image is obtained based on the average gray value of all the image regions in the first image;

[0015] The step of adjusting the current supplied to the multiple light source components according to the relevant grayscale value of the first image includes:

[0016] Obtain the difference between the average gray value of each image region and the average gray value of the first image;

[0017] The current supplied to each of the light source components is adjusted based on the difference between the average gray value of each of the image regions and the average gray value of the first image, and an adjustment constant; the adjustment constant is related to the current supplied to the first image region. The current of the light source component and the first image are related to the first... The average grayscale value of the corresponding image region of each of the aforementioned light source components is related.

[0018] Optionally, the adjustment formula for adjusting the current supplied to each light source component based on the difference between the average gray value of each image region and the average gray value of the first image, and an adjustment constant, is as follows:

[0019] I i1 =I i0 +(ΔG ri / Grav) / η,

[0020] In the formula, Ii1 represents the current of the i-th light source component after adjustment; Ii0 represents the current of the i-th light source component before adjustment; ΔGri represents the difference between the average gray value of the i-th image region and the average gray value of the first image; Grav represents the average gray value of the first image; and η represents the adjustment constant.

[0021] Optionally, the adjustment method further includes the following steps:

[0022] Obtain the adjustment constant.

[0023] Optionally, the step of obtaining the adjustment constant includes:

[0024] With all other light source components turned off, control is provided to the first... The current of each of the light source components changes from 0 to a maximum value according to a predetermined rule, and the first image corresponding to each current value is acquired;

[0025] Obtain the first image corresponding to the first... The average grayscale value of the image region of each of the aforementioned light source components;

[0026] Based on each of the current values ​​and the corresponding first image, the first... The average gray value of the image region of each of the light source components is obtained as a curve of average gray value changing with current, and a fitting equation is obtained, thereby obtaining the adjustment constant.

[0027] Optionally, the first image acquisition device further includes an image acquisition mechanism for acquiring images, the image acquisition mechanism including a lens; characterized in that the adjustment method further includes the following steps:

[0028] Adjust the focal length of the lens.

[0029] Optionally, the step of adjusting the focal length of the lens includes:

[0030] Obtain the distance between the image acquisition mechanism and the target object set on the carrier plate;

[0031] The focal length of the lens is adjusted according to the distance between the image acquisition mechanism and the target object.

[0032] Optionally, the step of adjusting the focal length of the lens further includes:

[0033] Adjust the position of the image acquisition mechanism in the Z direction.

[0034] Optionally, the first image acquisition device further includes an image acquisition mechanism for acquiring images; the adjustment method further includes the following steps:

[0035] Adjust the position of the image acquisition mechanism and the light source assembly in the X direction.

[0036] To achieve the above objectives, the present invention also provides an image acquisition device, including an image acquisition mechanism, a light source mechanism, and a control mechanism. The image acquisition mechanism is used to acquire images, and the light source mechanism includes multiple light source components and is used to provide illumination during the image acquisition process. The control mechanism is communicatively connected to the light source components and is configured to execute the adjustment method of the image acquisition device as described above.

[0037] Optionally, the light source mechanism further includes a base and n 2 One control circuit;

[0038] The number of light source components is n. 2 1, and n 2 Each of the light source components is disposed on the base and arranged in an n×n array; each light source component is connected to a control circuit, each control circuit is communicatively connected to the control mechanism and is configured to provide current to the corresponding light source component under the control of the control mechanism.

[0039] Optionally, each of the light source components includes m light sources, and the m×n light source structure... 2 The light sources are arranged in an n×(m×n) array.

[0040] Optionally, the first image acquisition device further includes a position adjustment mechanism, on which the light source mechanism and the image acquisition mechanism are mounted. The position adjustment mechanism is communicatively connected to the control mechanism and is at least used to adjust the position of the image acquisition mechanism in the Z direction under the control of the control mechanism.

[0041] To achieve the above objectives, the present invention also provides an immunoblotting detection system, including an analysis device and an image acquisition device as described above. The image acquisition device is used to acquire a second image of the immunoblotting detection membrane to be analyzed. The analysis device is communicatively connected to the image acquisition device and is configured to receive the second image and evaluate the characteristics of the protein detected by the immunoblotting detection membrane based on the second image.

[0042] To achieve the above objectives, the present invention also provides a computer-readable storage medium having a program stored thereon, which, when executed, performs the adjustment method of the image acquisition device as described above.

[0043] Compared with the prior art, the image acquisition device and adjustment method, the immunoblotting detection and analysis system, and the readable storage medium of the present invention have the following advantages:

[0044] The aforementioned image acquisition device includes an image acquisition mechanism, a light source mechanism, and a control mechanism. The image acquisition mechanism is used to acquire images. The light source mechanism includes multiple light source components and is used to provide illumination during the image acquisition process. The control mechanism is communicatively connected to the light source mechanism and is configured to execute an adjustment method to adjust the intensity of light provided by each of the light source components of the light source mechanism. The adjustment method includes the following steps: acquiring a first image of a target area on a carrier plate; acquiring the relevant grayscale value of the first image; and adjusting the current supplied to each light source component according to the relevant grayscale value of the first image, thereby achieving automatic adjustment of the intensity of light provided by each light source component. This configuration allows the light source mechanism to provide uniform illumination when the image acquisition mechanism acquires images, thereby improving the quality of the acquired images. Furthermore, since the control mechanism automatically adjusts the light intensity, no manual adjustment by the operator is required, making the image acquisition device convenient to use and providing accurate and reliable light intensity adjustment results.

[0045] The image acquisition device is applied to an immunoblotting detection system, which also includes an analysis device. The analysis device is communicatively connected to the image acquisition mechanism of the image acquisition device and is used to receive a second image of the immunoblotting detection membrane strip acquired by the image acquisition mechanism. Furthermore, the analysis device analyzes the second image of the immunoblotting detection membrane strip to evaluate the properties of the detected protein. By implementing the aforementioned adjustment method, the quality of the second image acquired by the image acquisition device is improved, resulting in more accurate and reliable analysis results when the analysis device evaluates the properties of the detected protein based on the second image. Attached Figure Description

[0046] The accompanying drawings are provided to better understand the invention and are not intended to unduly limit the scope of the invention. Wherein:

[0047] Figure 1 This is a schematic diagram of the structure of an image acquisition device provided according to an embodiment of the present invention;

[0048] Figure 2 This is a schematic diagram of the light source mechanism of the image acquisition device provided according to an embodiment of the present invention;

[0049] Figure 3 This is a schematic diagram of a first image acquired by an image acquisition device according to an embodiment of the present invention. The first image in the diagram is divided into multiple image regions.

[0050] Figure 4 This is a schematic diagram of the structure of the incubation tray used in the immunoblotting detection and analysis system provided according to an embodiment of the present invention.

[0051] [The annotations in the attached figures are explained below]:

[0052] 1000-Image acquisition mechanism, 1100-Lens, 2000-Light source mechanism, 2100-Light source assembly, 2110-Light source, 2200-Base, 3000-Control mechanism, 4000-Incubation tray, 4100-Incubation tank, 5000-Carrier plate, 6000-Position adjustment mechanism, 6100-First guide seat, 6200-First mounting bracket, 6300-First drive unit, 6400-Second mounting bracket, 6500-Second guide seat, 6600-Second drive unit;

[0053] 10 - Immunoblot detection strip, 20 - First image, 21 - Image region. Detailed Implementation

[0054] The following specific examples illustrate the implementation of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that the illustrations provided in this embodiment are only schematic representations of the basic concept of the present invention. Therefore, the drawings only show components related to the present invention and are not drawn according to the actual number, shape, and size of components in the actual implementation. In the actual implementation, the type, quantity, and proportion of each component can be arbitrarily changed, and the component layout may also be more complex.

[0055] Furthermore, while each embodiment described below possesses one or more technical features, this does not imply that users of the present invention must simultaneously implement all technical features in any embodiment, or can only separately implement some or all technical features in different embodiments. In other words, provided it is feasible, those skilled in the art can, based on the disclosure of the present invention and depending on design specifications or implementation requirements, selectively implement some or all technical features in any embodiment, or selectively implement a combination of some or all technical features in multiple embodiments, thereby increasing the flexibility in implementing the present invention.

[0056] As used herein, the singular forms “a,” “an,” and “the” include plural objects, and the plural form “multiple” includes two or more objects, unless otherwise expressly indicated. As used herein, the term “or” is generally used to include the meaning of “and / or,” unless otherwise expressly indicated, and the terms “installed,” “connected,” and “linked” should be interpreted broadly, for example, as a fixed connection, a detachable connection, or an integral connection. Connections can be mechanical or electrical. Connections can be direct or indirect through an intermediate medium, and can be internal communication between two elements or an interaction between two elements. Relational terms such as “first,” “second,” etc., are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations, nor do they indicate or imply relative importance or implicitly specify the number of indicated technical features. It should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "axial," "radial," and "circumferential," etc., indicating orientation or positional relationships, are based on the orientation or positional relationships shown in the accompanying drawings and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention. Those skilled in the art can understand the specific meaning of the above terms in the present invention according to the specific circumstances.

[0057] To make the objectives, advantages, and features of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings. It should be noted that the drawings are all in a very simplified form and use non-precise proportions, and are only used to facilitate and clearly illustrate the objectives of the embodiments of the present invention. The same or similar reference numerals in the drawings represent the same or similar parts.

[0058] This invention provides an image acquisition device that can be used in an immunoblotting analysis system. Figure 1 A schematic diagram of the image acquisition device is shown. Figure 1As shown, the image acquisition device includes an image acquisition mechanism 1000, a light source mechanism 2000, and a control mechanism 3000. The image acquisition mechanism 1000 is used to acquire images of an object. The light source mechanism 2000 includes multiple light source components 2100 and is used to provide illumination when the image acquisition mechanism 1000 acquires images of the object. The control mechanism 3000 is communicatively connected to the light source mechanism 2000 and is configured to execute an adjustment method to adjust the current supplied to each of the light source components 2100.

[0059] Those skilled in the art know that the intensity of the light provided by the light source component 2100 is directly proportional to the current value provided to the light source component 2100; that is, the magnitude of the current provided to the light source component 2100 determines the intensity of the light provided by the light source component 2100. Therefore, the control mechanism 3000 of the image acquisition device provided in this embodiment of the invention can automatically adjust the intensity of the light provided by each of the light source components 2100 of the light source mechanism 2000 by executing the adjustment method, so that the light source mechanism 2000 can uniformly illuminate the object to be acquired, thereby obtaining an object image with high quality. Since this adjustment method is executed automatically, it does not require manual adjustment by the operator, thus having the advantages of convenient use and accurate adjustment results. When the object to be acquired includes the immunoblot detection membrane strip 10 to be analyzed, it is beneficial to improve the analysis quality of the immunoblot detection membrane strip. The object to be acquired may also include an incubation tray 4000 for incubating the immunoblot detection membrane strip 10, a carrier plate 5000 for supporting the incubation tray 4000, etc. It should be understood that the object to be imaged is placed below the image acquisition mechanism 1000 and the light source mechanism 2000. Here, "below" includes both directly below and diagonally below.

[0060] In practice, the light source mechanism 2000 also includes a base 2200 and multiple control circuits (not shown in the figure). Multiple light source components 2100 are disposed on the base 2200, and each light source component 2100 is electrically connected to one of the control circuits. All control circuits are communicatively connected to the control mechanism 3000 and are used to provide current to the corresponding light source component 2100 under the control of the control mechanism 3000. In other words, in this embodiment of the invention, by independently controlling the current supplied to each light source component 2100 through corresponding control circuits, the intensity of the light provided by each light source component 2100 can be independently adjusted to better improve the uniformity of illumination provided by the light source mechanism 1000 to the object to be imaged, thereby improving the clarity of the captured image.

[0061] Optionally, the adjustment method specifically includes the following steps S100, S200, and S300. Step S100 includes acquiring a first image 20 of the target area on the carrier plate 5000 (e.g., ...). Figure 3 (As shown). Step S200 includes acquiring the relevant grayscale value of the first image 20. Step S300 includes adjusting the current supplied to each of the light source components 2100 according to the relevant grayscale value of the first image 20.

[0062] In step S100, the target area is, for example, the area corresponding to all the incubation tanks 4100 on the carrier plate 5000 when the incubation tray 4000 is placed on the carrier plate 5000. In practice, a mark can be set on the carrier plate 5000 to indicate the target area for easy identification.

[0063] In step S200, the relevant grayscale value is determined based on the specific configuration of the light source mechanism 2000. In a non-limiting embodiment, such as Figure 2 As shown, the light source mechanism 2000 includes n 2 Each of the aforementioned light source components 2100, and n 2 The light source components 2100 are arranged in an n×n array. Each light source component 2100 includes m light sources 2110, therefore the light source mechanism 2000 includes n 2 ×m light sources 2110. All the light sources 2110 are arranged in an n×(n×m) array. n is an odd number greater than 1, such as 3, 5, 7, or 9. Generally, the larger n is, the better the lighting uniformity of the light source mechanism 1000. However, this will increase the computational load of the control mechanism 3000, prolong the computation time, and make the configuration of the control mechanism 3000 higher, increasing the cost. Considering economic and practical aspects, n is preferably 3 (e.g., ...). Figure 2 (as shown) or 5. m is an integer greater than 1, such as 2, 3, or 4, etc.

[0064] Accordingly, such as Figure 3 As shown, the first image 20 is divided into n 2There are one image region 21, each image region 21 corresponding to one of the light source components 2100. Therefore, the relevant grayscale value includes the average grayscale value of the first image 20 and the average grayscale value of each image region 21. Step S200 includes steps S210 and S220. Step S210 includes obtaining the average grayscale value of each image region 21 in the first image 20. Step S220 includes obtaining the average grayscale value of the first image 20 based on the average grayscale values ​​of all image regions 21 in the first image 20. Specifically, it involves first obtaining the sum of the average grayscale values ​​of all image regions 21, then dividing the sum of the average grayscale values ​​of all image regions 21 by the number of image regions 21 n, and using the quotient as the average grayscale value of the first image 20.

[0065] In this case, step S300 includes steps S310 and S320. Step S310 includes obtaining the difference between the average gray value of each of the image regions 21 and the average gray value of the first image 20. Step S320 includes adjusting the current supplied to each of the light source components 2100 based on the difference between the average gray value of each of the image regions 21 and the average gray value of the first image 20 and a known adjustment constant η.

[0066] That is, the current supplied to the first light source component 2100 is adjusted according to the difference between the average gray value of the first image region 21 and the average gray value of the first image 20; the current supplied to the second light source component 2100 is adjusted according to the difference between the average gray value of the second image region 21 and the average gray value of the first image 20; and so on, the current supplied to the (n-1)th light source component 2100 is adjusted according to the difference between the average gray value of the (n-1)th image region 21 and the average gray value of the first image 20. The specific current adjustment formula is I... i1 =I i0 +(ΔG ri / Grav) / η, where I i1 I represents the adjusted current of the i-th light source component; i0 The current of the i-th light source component before adjustment is represented; Grav represents the average gray value of the first image 20; ΔGr i η represents the difference between the average gray value of the i-th image region 21 and the average gray value of the first image 20; η represents the adjustment constant.

[0067] In practice, step S210 is executed at least once. If the average grayscale value of all image regions 21 in the first image 20 is equal, steps S220 and S300 do not need to be executed further. In other words, when the average grayscale value of all image regions 21 in the first image 20 is equal, it is considered that the light source mechanism 1000 has uniformly illuminated the object in the image to be acquired.

[0068] The adjustment constant and the provided The current of the light source component 2100 and the first image 20 This is related to the average gray value of each of the image regions 21. In some embodiments, the adjustment constant may be pre-stored in the control mechanism 3000; in other embodiments, step S400 may be performed before step S100 to obtain the adjustment constant η.

[0069] Step S400 includes steps S410, S420, and S430. Step S410 includes controlling the supply to the first light source 2100 while all other light source components 2100 are turned off. The current of each of the light source components 2100 changes from 0 to its maximum value according to a predetermined rule, and the first image 20 corresponding to each current value is acquired. Step S420 includes acquiring the first current value in each of the first images 20. The average grayscale value of each of the image regions 21. Step S430 includes determining the average grayscale value of each of the current values ​​and the corresponding number of current values ​​in the first image 20. The average gray value of the first image region 21 is obtained. The curves of the average gray value of each image region 21 changing with the current were obtained, and the fitting equation was: Gr=ηI, from which the adjustment constant was obtained.

[0070] The "predetermined rule" mentioned in step S410 is, for example, provided by control to the first time step at the first moment. The current of the first light source component 2100 is 0 (i.e., the first... (The light source component 2100 is not turned on), and then at the second moment, control is provided to the first... The current of the light source component 2100 is 1, and at the third moment, the control provides the current to the first... The current of the light source component 2100 is 2... until at time p, the control provides the current to the... The current supplied to the first light source component 2100 is p, where p is the maximum current. Of course, at two adjacent moments, the current supplied to the first... The difference in current between the light source components 2100 can also be other values, or any two adjacent moments, provided to the first... The current differences of the light source components 2100 can be equal or unequal. Furthermore, this embodiment of the invention does not impose any special limitation on the time difference between two adjacent moments.

[0071] The image acquisition mechanism 1000 is communicatively connected to the control mechanism 3000 and operates under the control of the control mechanism 3000. The image acquisition mechanism 1000 includes a lens 1100. It is worth noting that adjustments to the light source mechanism 200 should be made while the lens 1100 of the image acquisition mechanism 1000 is focused. Therefore, the adjustment method may further include step S500: controlling the image acquisition mechanism 1000 to operate in order to adjust the focal length of the lens 1100. Step S500 is performed before step S100.

[0072] The image acquisition mechanism 1000 includes an autofocus camera. When the image acquisition mechanism 1000 and the target object are within the autofocus range of the autofocus camera, step S500 may include steps S510 and S520. Step S510 obtains the distance between the image acquisition mechanism 1000 and the target object on the carrier plate 5000. The target object is, for example, an immunoblot detection membrane strip 10 to be analyzed. It is understood that the immunoblot membrane strip is placed in the incubation tank 4100 of the incubation tray 4000, and the incubation tray 4000 is placed on the carrier plate 5000. Step S520 includes the autofocus camera automatically focusing based on the distance between the image acquisition mechanism 1000 and the target object. The focusing formula is: L=S / (1+H / C), where L represents the focal length of the autofocus camera, S represents the distance between the autofocus camera and the target object, H represents the length of the target object, and C represents the width of the CCD in the autofocus camera, which is an inherent parameter of the autofocus camera.

[0073] It should be noted that the autofocus camera can be a commercially available autofocus camera product, or it can be assembled by those skilled in the art using a non-focusable ordinary camera, a rangefinder module, a lens, and a microprocessor based on the principle of autofocus. For those skilled in the art, combining the ordinary camera, the rangefinder module, the lens, and the microprocessor to achieve the autofocus function of the image acquisition device is common knowledge and will not be elaborated here.

[0074] When the distance between the image acquisition mechanism 1000 and the target object is too large or too small, causing the distance between the image acquisition mechanism 1000 and the target object to be outside the focusing range of the autofocus camera, step S500 further includes step S530. Step S530 includes the control mechanism 3000 adjusting the position of the image acquisition mechanism 1000 in the Z direction according to the distance between the image acquisition mechanism 1000 and the target object, so that the distance between the image acquisition mechanism 1000 and the target object is within the focusing range of the autofocus camera. The Z direction can be a vertical direction.

[0075] Therefore, the image acquisition device further includes a position adjustment mechanism 6000, which includes a first guide seat 6100, a first mounting bracket 6200, and a first drive unit 6300. The first mounting bracket 6200 is movably mounted on the first guide seat 6100, and the first drive unit 6300 is connected to the first mounting bracket 6200 and is also communicatively connected to the control mechanism 3000. The first drive unit 6300, under the control of the control mechanism 3000, drives the first mounting bracket 6200 to reciprocate linearly along the Z-direction on the first guide seat 6100. The image acquisition mechanism 1000 is connected to the first mounting bracket 6200. Thus, the image acquisition mechanism 1000 can reciprocate linearly along the Z-direction on the first guide seat 6100 together with the first mounting bracket 6200, achieving position adjustment in the Z-direction. In this embodiment of the invention, the cooperation between the first guide seat 6100 and the first drive unit 6300 can be any suitable linear drive module in the prior art. For example, the first guide seat 6100 includes a lead screw and the first drive unit 6300 includes a motor, or the first guide seat 6100 includes a guide rail and the first drive unit 6300 includes an electric telescopic rod or a telescopic cylinder, etc.

[0076] As mentioned above, the image acquisition device is used in an immunoblotting analysis system. In practical applications, such as... Figure 4 As shown, the incubation tray 4000 placed on the carrier plate 5000 includes multiple incubation grooves 4100, which are arranged at intervals along the X direction. Therefore, the incubation tray 4000 can simultaneously incubate multiple immunoblot detection membrane strips 10. This requires the image acquisition device to be able to sequentially acquire images of multiple immunoblot detection membrane strips 10. The X direction is perpendicular to the Z direction and is a horizontal direction.

[0077] Therefore, the adjustment method further includes adjusting the positions of the image acquisition mechanism 1000 and the light source mechanism 2000 in the X direction, so that the image acquisition mechanism 1000 can sequentially acquire images of each of the immunoblot detection membrane strips 10, and the light source mechanism 2000 can sequentially and uniformly illuminate each of the immunoblot detection membrane strips 10. The distance of each adjustment is equal to the distance between two adjacent immunoblot detection membrane strips 10.

[0078] Accordingly, the position adjustment mechanism 6000 further includes a second mounting bracket 6400, a second guide seat 6500, and a second drive unit 6600. The second mounting bracket 6400 is mounted on the first guide seat 6100 and is connected to the light source mechanism 2000. The first guide seat 6100 is movably mounted on the second guide seat 6500. The second drive unit 6600 is connected to the first guide seat 6100 and is communicatively connected to the control mechanism 3000. Under the control of the control mechanism 3000, the second drive unit 6600 drives the first guide seat 6100 to reciprocate linearly along the X direction on the second guide seat 6500 to adjust the position of the image acquisition mechanism 1000 and the light source mechanism 2000 in the X direction. Here, the second mounting bracket 6400 can also be movably connected to the first guide seat 6100 and connected to the first drive unit 6300, and is used to move along the Z direction under the drive of the first drive unit 6300 to adjust the position of the light source mechanism 2000 in the Z direction.

[0079] When the image acquisition device is applied to an immunoblotting detection and analysis system, the immunoblotting detection and analysis system further includes an analysis device. The analysis device is communicatively connected to the image acquisition mechanism 1000 of the image acquisition device and is used to receive a second image of the immunoblotting detection membrane strip 10 acquired by the image acquisition mechanism 1000, and to analyze the second image to evaluate the properties of the protein detected by the immunoblotting detection membrane strip. Because the image acquisition device performs the aforementioned adjustment method, the quality of the acquired second image is improved. Therefore, when the analysis device evaluates the properties of the detected protein based on the analysis of the second image, the evaluation results are more accurate and reliable.

[0080] With the adjustment constant pre-stored in the control mechanism 3000, the usage process of the immunoblotting detection and analysis system is as follows:

[0081] First, place one of the immunoblot detection membrane strips 10 into any one of the incubation tanks 4100 in the incubation tray 4000, and then place the incubation tray 4000 on the carrier plate 5000.

[0082] Then, step S500 of the aforementioned adjustment method is performed so that the image acquisition mechanism 1000 focuses on the immunoblot detection membrane strip 10.

[0083] The incubation tray 4000 was then removed.

[0084] Then, steps S100 to S300 of the adjustment method are performed.

[0085] Next, the incubation tray 4000, carrying multiple immunoblot detection membrane strips 10, is placed on the carrier plate 5000. Then, the control mechanism 3000 controls the image acquisition mechanism 1000 to acquire a second image of the first immunoblot detection membrane strip 10. Then, step S600 in the adjustment method is executed. After step S600 is completed, the image acquisition mechanism 1000 is controlled to acquire a second image of the second immunoblot detection membrane strip 10. Then, step S600 is executed again. That is, the step of acquiring the second image of the immunoblot detection membrane strip 10 is performed alternately with step S600 until the acquisition of the second images of all the immunoblot detection membrane strips 10 is completed.

[0086] Next, the analysis device analyzes the received second image of the immunoblot detection membrane strip 10 and evaluates the properties of the detected protein.

[0087] Furthermore, embodiments of the present invention also provide a computer-readable storage medium having a program stored thereon, which, when executed, performs the adjustment method of the image acquisition device as described above.

[0088] In summary, the image acquisition device provided by this invention automatically adjusts the light intensity emitted by each light source component of the light source mechanism through the control unit executing the adjustment method, achieving uniform illumination of the object to be imaged. This improves the quality of the acquired image without requiring manual adjustment by the operator, offering advantages such as ease of use and reliable adjustment results. When the image acquisition device is applied to an immunoblotting detection system, and the analysis device is used to analyze the second image of the immunoblotting detection membrane strip acquired by the image acquisition device to evaluate the properties of the detected protein, the improved quality of the second image leads to more accurate and reliable evaluation results.

[0089] While the present invention has been disclosed above, it is not limited thereto. Those skilled in the art can make various modifications and variations to the present invention without departing from its spirit and scope. Therefore, if such modifications and variations fall within the scope of the present invention and its equivalents, the present invention also intends to include such modifications and variations.

Claims

1. A method for adjusting an image acquisition device, applied to an immunoblotting analysis system, wherein the image acquisition device includes multiple light source components for illumination, the number of which is n. 2 1, and n 2 The light source components are arranged in a manner that Array; each of the light source components includes m light sources, and all the light sources are arranged in an array. An array, where n is an odd number greater than 1 and m is an integer greater than 1; characterized in that... Includes the following steps: Acquire a first image of the target area on the carrier plate; the target area is the area corresponding to all the incubation slots on the carrier plate and the incubation tray when the incubation tray is placed on the carrier plate; Obtain the relevant grayscale values ​​of the first image; and, The current supplied to each of the light source components is adjusted according to the relevant grayscale value of the first image; The relevant grayscale value includes the average grayscale value of the first image and the average grayscale value of the image region corresponding to each of the light source components in the first image; the step of adjusting the current supplied to each of the light source components according to the relevant grayscale value of the first image includes: Obtain the difference between the average gray value of each image region and the average gray value of the first image; The current supplied to each of the light source components is adjusted based on the difference between the average gray value of each of the image regions and the average gray value of the first image, and an adjustment constant; the adjustment constant is related to the current supplied to the first image region. +1 The current of the light source component and the first image with respect to the first The average grayscale value of the corresponding image region of the +1 light source component is related; When the average grayscale value of all the image regions is equal, the current adjustment of each of the light source components is completed; The adjustment formula for adjusting the current supplied to each light source component based on the difference between the average gray value of each image region and the average gray value of the first image, and an adjustment constant, is as follows: ， In the formula, I i1 I represents the adjusted current of the i-th light source component; i0 ΔGri represents the current of the i-th light source component before adjustment; ΔGri represents the difference between the average gray value of the i-th image region and the average gray value of the first image; Grav represents the average gray value of the first image; η represents the adjustment constant. The step of obtaining the adjustment constant includes: under the condition that all other light source components are turned off, controlling the supply to the first... The current of each of the +1 light source components changes from 0 to a maximum value according to a predetermined rule, and the first image corresponding to each current value is acquired; Obtain the first image corresponding to the first... +1 average grayscale value of the image region of the light source component; Based on each of the current values ​​and the corresponding first image, the first... The average gray value of the image region of the +1 light source components is used to obtain the curve of the average gray value changing with the current, and a fitting equation is obtained, thereby obtaining the adjustment constant.

2. The adjustment method of the image acquisition device according to claim 1, characterized in that, The step of obtaining the relevant grayscale value of the first image includes: Obtain the average grayscale value of the image region corresponding to each of the light source components in the first image; The average gray value of the first image is obtained based on the average gray value of all the image regions in the first image.

3. The adjustment method of the image acquisition device according to claim 1 or 2, characterized in that, The adjustment method further includes the following steps: Obtain the adjustment constant.

4. The adjustment method of the image acquisition device according to claim 1, characterized in that, The image acquisition device further includes an image acquisition mechanism for acquiring images, the image acquisition mechanism including a lens; characterized in that the adjustment method further includes the following steps: Adjust the focal length of the lens.

5. The adjustment method of the image acquisition device according to claim 4, characterized in that, The step of adjusting the focal length of the lens includes: Obtain the distance between the image acquisition mechanism and the target object set on the carrier plate; The focal length of the lens is adjusted according to the distance between the image acquisition mechanism and the target object.

6. The adjustment method of the image acquisition device according to claim 5, characterized in that, The step of adjusting the focal length of the lens further includes: Adjust the position of the image acquisition mechanism in the Z direction.

7. The adjustment method of the image acquisition device according to claim 1, characterized in that, The image acquisition device further includes an image acquisition mechanism for acquiring images; the adjustment method further includes the following steps: Adjust the position of the image acquisition mechanism and the light source assembly in the X direction.

8. An image acquisition device, characterized in that, It includes an image acquisition mechanism, a light source mechanism, and a control mechanism. The image acquisition mechanism is used to acquire images, and the light source mechanism includes multiple light source components to provide illumination during the image acquisition process. The light source mechanism also includes a base and n 2 One control circuit; the number of light source components is n. 2 1, and n 2 The aforementioned light source components are disposed on the base and arranged in a manner that... Array; each of the light source components includes m light sources, and the light source mechanism in The light sources are arranged in An array; each of the light source components is connected to one of the control circuits, each of the control circuits is communicatively connected to the control mechanism and is configured to provide current to the corresponding light source component under the control of the control mechanism; The control mechanism is communicatively connected to the light source assembly and is configured to perform the adjustment method of the image acquisition device as described in claim 1.

9. The image acquisition device according to claim 8, characterized in that, The image acquisition device further includes a position adjustment mechanism, on which the light source mechanism and the image acquisition mechanism are mounted. The position adjustment mechanism is communicatively connected to the control mechanism and is used at least to adjust the position of the image acquisition mechanism in the Z direction under the control of the control mechanism.

10. An immunoblotting detection system, characterized in that, The device includes an analysis apparatus and an image acquisition apparatus as described in claim 8 or 9, the image acquisition apparatus being used to acquire a second image of an immunoblot detection membrane to be analyzed, the analysis apparatus being communicatively connected to the image acquisition apparatus and configured to receive the second image and evaluate the characteristics of the protein detected by the immunoblot detection membrane based on the second image.

11. A computer-readable storage medium having a program stored thereon that, when executed, performs the adjustment method of the image acquisition device as claimed in claim 1.

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