A quercetin / polycaprolactone fiber electrospun membrane and its preparation method and application

Abstract

The present invention provides a quercetin / polycaprolactone fiber electrospun membrane, a preparation method, and applications thereof. The preparation method comprises the following steps: 1) dispersing quercetin powder and polycaprolactone in a dispersant and mixing them uniformly to obtain a spinnable solution; and 2) subjecting the spinnable solution to an electrospinning process to produce the quercetin / polycaprolactone fiber electrospun membrane. The present invention utilizes quercetin, a natural flavonoid compound, as a functional raw material, combined with a high molecular weight organic polymer, and produced by an electrospinning process to produce the quercetin / polycaprolactone fiber electrospun membrane. The fiber electrospun membrane exhibits excellent surface morphology, mechanical properties, biodegradability, biocompatibility, and the ability to induce osteogenic differentiation of stem cells. It can be used as a biomembrane to guide periodontal tissue regeneration.

Description

Technical Field

[0001] The present invention relates to the technical field of biomaterials, and in particular to a quercetin / polycaprolactone fiber electrospun membrane and a preparation method and application thereof. Background Art

[0002] Periodontitis is a common chronic inflammatory disease affecting approximately 30-40% of the world's population. It can damage periodontal supporting tissues such as the gums, periodontal ligament, alveolar bone, and cementum, leading to alveolar bone resorption and even tooth loss. Therefore, controlling periodontal inflammation and restoring the structure and function of damaged periodontal tissues are key to the clinical treatment of periodontitis.

[0003] Guided tissue regeneration (GTR) is one of the classic methods of periodontal regenerative therapy. The biofilm used for GTR must have the function of a barrier membrane to prevent soft tissue from migrating into the periodontal defect site and provide sufficient space for periodontal tissue regeneration. Traditional GTR biofilms include absorbable and non-absorbable membranes. Non-absorbable membranes require a second surgery to remove, causing damage to the newly formed tissue. In addition, the existing absorbable GTR membranes lack the ability to resist inflammation and promote periodontal tissue regeneration. Therefore, it is crucial for periodontal regenerative therapy to construct a biofilm that can inhibit periodontal inflammatory response, promote osteogenic / angiogenic differentiation of periodontal ligament stem cells (PDLSCs), and have good mechanical properties and a controllable degradation rate. Summary of the Invention

[0004] In view of the above-mentioned shortcomings of the prior art, the object of the present invention is to provide a quercetin / polycaprolactone fiber electrospun membrane and its preparation method and application, which are used to solve the problem that the absorbable GTR membrane in the prior art lacks the anti-inflammatory and periodontal tissue regeneration promoting functions.

[0005] To achieve the above-mentioned purpose and other related purposes, the present invention is obtained by including the following technical solutions.

[0006] One of the objects of the present invention is to provide a method for preparing a quercetin / polycaprolactone fiber electrospun membrane, comprising the following steps:

[0007] 1) dispersing quercetin powder and polycaprolactone in a dispersant and mixing them uniformly to obtain a spinnable solution;

[0008] 2) The spinnable solution was prepared by electrospinning process to obtain quercetin / polycaprolactone fiber electrospun membrane.

[0009] Preferably, the dispersant is selected from one or more of hexafluoroisopropanol, tetrahydrofuran and toluene.

[0010] Preferably, the number average molecular weight of the polycaprolactone is 6,000 to 10,000.

[0011] Preferably, the amount of the quercetin powder added to the dispersant is 0.005-0.008 g / mL, more preferably 0.0064 g / mL.

[0012] Preferably, the amount of polycaprolactone added to the dispersant is 0.10 to 0.20 g / mL, more preferably 0.16 g / mL.

[0013] Preferably, the electrospinning process is carried out under the following conditions: the feed rate of the spinnable solution is 1 to 1.5 ml / h.

[0014] Preferably, the electrospinning time is 1 to 3 hours.

[0015] Preferably, a drum is used as the collecting device, and the collecting rotation speed is 8 to 12 r / min.

[0016] Preferably, the spinning voltage in the electrospinning is 12 to 25 kV, the collecting distance is 20 to 25 cm, and the reciprocating distance is 15 to 20 cm.

[0017] More preferably, the spinning voltage in the electrospinning is 20 kV, the collecting distance is 25 cm, and the reciprocating distance is 17 cm.

[0018] Preferably, the temperature of the electrospinning is 20-30° C., and the humidity is 30-40%.

[0019] The second object of the present invention is to provide a quercetin / polycaprolactone fiber electrospun membrane, wherein the quercetin / polycaprolactone fiber electrospun membrane contains quercetin and polycaprolactone, and the mass ratio of the quercetin to the polycaprolactone is (5-8):(100-200).

[0020] Preferably, the diameter of the fibers in the quercetin / polycaprolactone fiber electrospun membrane is 1.5 to 2.5 μm.

[0021] Preferably, the thickness of the quercetin / polycaprolactone fiber electrospun membrane is 35 to 70 μm, preferably 50 to 55 μm, and the tensile strength of the quercetin / polycaprolactone fiber electrospun membrane is 2.3 to 3.3 MPa.

[0022] Preferably, the quercetin / polycaprolactone fiber electrospun membrane is obtained by the above-mentioned preparation method.

[0023] A third object of the present invention is to provide a quercetin / polycaprolactone fiber electrospun membrane for use as a biomembrane to guide periodontal tissue regeneration.

[0024] As described above, the quercetin / polycaprolactone fiber electrospun membrane of the present invention and its preparation method and application have the following beneficial effects: the natural flavonoid compound quercetin is used as the functional raw material, and the quercetin / polycaprolactone fiber electrospun membrane is prepared by combining a high molecular organic polymer with an electrospinning process. The fiber electrospun membrane has good surface morphology, mechanical properties, degradability, biocompatibility and the effect of inducing osteogenic differentiation of stem cells, and can be used as an absorbable guided tissue regeneration membrane in the field of periodontal regeneration. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 Shown is the TEM image of the quercetin powder in Example 1.

[0026] Figure 2 Shown is a TEM image of polycaprolactone in Example 1.

[0027] Figure 3 Shown is the TEM image of the quercetin / polycaprolactone fiber electrospun membrane prepared in Example 1.

[0028] Figure 4 Shown are the SEM images (A) and diameter distribution diagram (B) of the polycaprolactone and quercetin / polycaprolactone fiber electrospun membranes in Example 1.

[0029] Figure 5 Shown are Fourier transform infrared spectra of quercetin powder, polycaprolactone and quercetin / polycaprolactone fiber electrospun membrane in Example 1.

[0030] Figure 6 Shown are the mechanical property test results of the polycaprolactone and quercetin / polycaprolactone fiber electrospun membranes in Example 1: A is the tensile strength; B is the Young's modulus; C is the elongation at break; and D is the stress-strain curve.

[0031] Figure 7 Shown are the contact angle test results of the polycaprolactone and quercetin / polycaprolactone fiber electrospun membranes in Example 1.

[0032] Figure 8 Shown are the degradation rates of the quercetin / polycaprolactone fiber electrospun membrane in Example 1 in a sodium hydroxide solution (A) with a pH of 8.5 and artificial saliva (B) over time.

[0033] Figure 9 Shown are the test results of the biocompatibility experiment of the quercetin / polycaprolactone fiber electrospun membrane in Example 1: A is a live and dead cell staining image; B is a cytoskeleton staining image; C is a CCK-8 detection image.

[0034] Figure 10The results show the effect of the quercetin / polycaprolactone fiber electrospun membrane in Example 1 on the osteogenic differentiation of hPDLSCs: A is semi-quantitative analysis of alkaline phosphatase; B is alkaline phosphatase staining. DETAILED DESCRIPTION

[0035] The following describes the embodiments of the present invention through specific examples. Those skilled in the art will readily understand the other advantages and benefits of the present invention from the disclosure herein. The present invention may also be implemented or applied through various other specific embodiments, and the details in this specification may be modified or altered based on different viewpoints and applications without departing from the spirit of the present invention.

[0036] It should be noted that the process equipment or devices not specifically specified in the following embodiments are all conventional equipment or devices in the art.

[0037] Furthermore, it should be understood that the one or more method steps mentioned in the present invention do not exclude the presence of other method steps before or after the combination step, or the insertion of other method steps between these explicitly mentioned steps, unless otherwise specified. It should also be understood that the combination connection relationship between one or more devices / apparatuses mentioned in the present invention does not exclude the presence of other devices / apparatuses before or after the combination device / apparatus, or the insertion of other devices / apparatuses between two explicitly mentioned devices / apparatuses, unless otherwise specified. Furthermore, unless otherwise specified, the numbering of each method step is merely a convenient tool for identifying each method step, and is not intended to limit the order of arrangement of each method step or to define the scope of the present invention. Changes or adjustments to their relative relationships, without substantially changing the technical content, should also be considered within the scope of the present invention.

[0038] The present invention provides a method for preparing a quercetin / polycaprolactone fiber electrospun membrane, comprising the following steps:

[0039] 1) dispersing quercetin powder and polycaprolactone in a dispersant and mixing them uniformly to obtain a spinnable solution;

[0040] 2) The spinnable solution was prepared by electrospinning process to obtain quercetin / polycaprolactone fiber electrospun membrane.

[0041] In the above technical solution of the present application, quercetin is a natural flavonoid compound that is widely found in plants such as fruits and vegetables, and has anti-inflammatory, antioxidant, and other effects. Quercetin can promote the osteogenic / angiogenic differentiation of bone marrow mesenchymal stem cells and promote bone defect repair in vivo. Quercetin can inhibit the inflammatory response of chondrocytes under an inflammatory microenvironment and maintain the chondrocyte phenotype under an inflammatory microenvironment, inhibiting the progression of knee osteoarthritis in rats. It can be seen that quercetin has both anti-inflammatory and osteopromoting effects and is expected to be applied to periodontal regeneration therapy. Electrospinning technology is a technology that uses an electrostatic field to overcome the surface tension and viscoelastic force of a polymer solution, causing it to stretch and bend under the action of electrostatic force, thereby obtaining a fiber membrane. Electrospun membranes can simulate the microenvironment of the extracellular matrix and support cell growth, while having good elasticity and a smaller pore size, and can support cell migration across the membrane.

[0042] In a specific embodiment, the dispersion is selected from one or more of hexafluoroisopropanol, tetrahydrofuran and toluene.

[0043] Polycaprolactone (PCL) is a high-molecular-weight organic polymer with excellent biocompatibility, biodegradability, and mechanical properties. Its tightly packed molecular chains prevent the ingress of external fluids and degrade slowly. Therefore, electrospun polycaprolactone fiber membranes are expected to be ideal for GTR applications in periodontal regeneration.

[0044] In a specific embodiment, the number average molecular weight of the polycaprolactone is 6,000 to 10,000.

[0045] In a specific embodiment, the amount of the quercetin powder added to the dispersant is 0.005-0.008 g / mL, such as specifically 0.0064 g / mL.

[0046] In a specific embodiment, the amount of polycaprolactone added to the dispersant is 0.10-0.20 g / mL, such as specifically 0.16 g / mL.

[0047] In a specific embodiment, the electrospinning process is performed under the following conditions: the feed rate of the spinnable solution is 1 to 1.5 ml / h.

[0048] In a specific embodiment, the electrospinning time is 1 to 3 hours.

[0049] In a specific embodiment, a drum is used as the collecting device, the collecting rotation speed is 8 to 12 r / min, and the diameter of the drum is 8 cm.

[0050] In a specific embodiment, the spinning voltage in the electrospinning is 12-25 kV, such as 12-15 kV, 15-20 kV, 20-25 kV; the collection distance is 20-25 cm, and the reciprocating distance is 15-20 cm.

[0051] In a more specific embodiment, the positive electrode voltage is +6 to +10 kV, and the negative electrode voltage is -10 to -6 kV.

[0052] In a specific embodiment, the spinning voltage in the electrospinning is 20 kV, the collecting distance is 25 cm, and the reciprocating distance is 17 cm.

[0053] In a more specific embodiment, the conditions of the electrospinning process are as follows: the spinnable solution is transferred to a syringe with a capacity of 10 ml and a needle size of 21G, a flow pump is used to control the solution propulsion speed at 1 ml / h, the spinning voltage is 20 kV, the spinning time is 3 h, a roller with a diameter of 8 cm is used as a collection device, the positive and negative electrode voltages are +10 kV and -10 kV respectively, and the collection distance is 25 cm.

[0054] In a specific embodiment, the temperature of the electrospinning is 20-30° C., and the humidity is 30-40%.

[0055] The present application also provides a specific quercetin / polycaprolactone fiber electrospun membrane, wherein the quercetin / polycaprolactone fiber electrospun membrane contains quercetin and polycaprolactone, and the mass ratio of the quercetin to the polycaprolactone is (5-8):(100-200), such as 5:200, 6.4:160, and 8:100.

[0056] In a specific embodiment, the diameter of the fibers in the quercetin / polycaprolactone electrospun fiber membrane is 1.5 to 2.5 μm.

[0057] In a specific embodiment, the thickness of the quercetin / polycaprolactone fiber electrospun membrane is 35-70 μm, such as 35-50 μm, 50-55 μm, or 55-70 μm. The tensile strength of the quercetin / polycaprolactone fiber electrospun membrane is 2.3-3.3 MPa.

[0058] In a specific embodiment, the quercetin / polycaprolactone fiber electrospun membrane is obtained by the above-mentioned preparation method.

[0059] The embodiments of the present application also provide a use of a quercetin / polycaprolactone fiber electrospun membrane as a biomembrane for guiding periodontal tissue regeneration.

[0060] The following describes the implementation of the present invention through specific embodiments. People skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.

[0061] Example 1

[0062] In the embodiment of the present application, the dispersant is hexafluoroisopropanol (Macklin, H811026, Shanghai), and the high molecular organic polymer is polycaprolactone (Sigma, 440744, USA) with a number average molecular weight of 8000. The TEM image thereof is shown in FIG. Figure 2 shown.

[0063] The present invention provides a method for preparing a quercetin / polycaprolactone fiber electrospun membrane, comprising the following steps:

[0064] A) 0.064 g of quercetin powder (Sigma, Q4951, USA) was weighed and added to 10 ml of hexafluoroisopropanol (HFIP). The mixture was shaken in an ultrasonic oscillator for 30 min to obtain a quercetin dispersion.

[0065] B) adding 1.6 g of polycaprolactone particles (PCL) to the quercetin dispersion and stirring on a magnetic stirrer for 12 h to obtain a spinnable solution;

[0066] C) At 25°C and 30% humidity, the spinnable solution was transferred to a 10 ml syringe with a 21G needle. A flow pump was used to control the solution propulsion rate at 1 ml / h. The spinning voltage was 20 kV, and the spinning time was 3 h. A drum with a diameter of 8 cm was used as a collection device. The positive and negative voltages were +10 kV and -10 kV, respectively. The collection distance was 25 cm, and the reciprocating distance was 17 cm. After the spinning was completed, the electrospun membrane was removed from the drum and placed in a fume hood for 2.5 days to allow the organic solvent HFIP to fully evaporate. A quercetin / polycaprolactone fiber electrospun membrane with a thickness of 60 μm was obtained, which was recorded as 4% Qtn / PCL fiber.

[0067] The TEM images of quercetin, polycaprolactone and quercetin / polycaprolactone fiber electrospun membranes in this embodiment are shown in FIG. Figure 1-3 As shown in Figure 2, the SEM images and fiber diameter distribution of polycaprolactone and quercetin / polycaprolactone fiber electrospun membranes are shown in Figure 2. Figure 4 As shown, from Figure 3 It can be seen from the figure that quercetin particles are distributed in the 4% Qtn / PCL fibers. Figure 4 B It can be seen that the average diameter of pure PCL is 1.762±0.219μm; the average diameter of the fibers in the quercetin / polycaprolactone fiber electrospun membrane is 1.896±0.222μm.

[0068] The Fourier transform infrared spectra of quercetin powder, polycaprolactone and quercetin / polycaprolactone fiber electrospun membrane are shown in Figure 2. Figure 5 As shown, combined Figure 5 It can be seen that quercetin has been successfully embedded in the PCL fiber filaments. In this example, a quercetin / polycaprolactone fiber electrospun membrane was successfully prepared.

[0069] The 4% Qtn / PCL fiber sample and pure PCL prepared in this example were tested using a universal mechanical testing machine at a test speed of 5.0 mm / min. The results are shown in Figure 2. Figure 6 The test results show that the tensile strength of the 4% Qtn / PCL fiber electrospun membrane prepared in this example can be controlled within the range of 2.3 to 3.3 MPa. The tensile strength and elastic modulus of the 4% Qtn / PCL fiber electrospun membrane are superior to those of the pure PCL group, and the elongation at break is lower than that of the pure PCL group, indicating that the prepared Qtn / PCL fiber electrospun membrane has better tensile properties.

[0070] The 4% Qtn / PCL fiber sample and pure PCL prepared in this example were tested using a water contact angle meter. The results are as follows: Figure 7 The test results show that the contact angle of the Qtn / PCL fiber electrospun membrane obtained by the present invention can be controlled within the range of 127° to 127.5°, with no significant difference from the PCL group, proving that the addition of quercetin powder does not affect the hydrophobic properties of polycaprolactone itself.

[0071] The 4% Qtn / PCL fiber samples prepared in this example were washed with deionized water and dried before being tested for biodegradability. The 4% Qtn / PCL fiber samples were immersed in a sodium hydroxide solution (pH 8.5) and artificial saliva (AS) at 37°C for different time periods, then rinsed with deionized water and dried. The samples were weighed (Mt), the initial weight (M0), and the degradation rate was [(M0-Mt) / M0] x 100%. The degradation rate was used to evaluate the degradation rate and biodegradability of the material. The results are shown in Figure 2. Figure 8 The test results show that the degradation rate of the 4% Qtn / PCL fiber electrospun membrane of the present invention can reach about 10% when immersed in a sodium hydroxide solution with a pH value of 8.5 and artificial saliva for 35 days, which shows that the quercetin / polycaprolactone fiber electrospun membrane of the present invention has certain biodegradability.

[0072] The 4% Qtn / PCL fiber sample prepared in this example was washed with deionized water, dried, and sterilized before biocompatibility testing. hPDLSCs were seeded on the 4% Qtn / PCL fiber electrospun membrane prepared in this example, and CCK-8 assay was performed 1, 3, and 5 days after seeding ( Figure 9 C), Live / dead cell staining and cytoskeleton staining were performed 3 days after seeding ( Figure 9 A, Figure 9 B) CCK-8 and live-dead cell staining results showed that the 4% Qtn / PCL fiber sample promoted the proliferation of hPDLSCs. Phalloidin staining results showed that hPDLSCs adhered well to the 4% Qtn / PCL fiber electrospun membrane. These results demonstrate the good biocompatibility of the quercetin / polycaprolactone fiber electrospun membrane of the present invention.

[0073] The 4% Qtn / PCL fiber sample prepared in this example was washed with deionized water, dried, and sterilized. hPDLSCs were then seeded on the electrospun membrane and cultured for 7 days. After 7 days, alkaline phosphatase (ALP) staining and semi-quantitative detection ( Figure 10 A, Figure 10 B). ALP staining and semi-quantitative results showed that compared with the pure PCL group, the 4% Qtn / PCL fiber electrospun membrane promoted the expression of alkaline phosphatase in hPDLSCs, promoted the osteogenic differentiation of hPDLSCs, and had certain potential in the field of periodontal regeneration. In summary, the present invention uses the natural flavonoid compound quercetin as a functional raw material, combined with a high molecular organic polymer, and adopts an electrospinning process to prepare a quercetin / polycaprolactone fiber electrospun membrane. The fiber electrospun membrane has good surface morphology, mechanical properties, degradability, biocompatibility, and the ability to induce osteogenic differentiation of stem cells. It can be used as an absorbable guided tissue regeneration membrane in the field of periodontal regeneration. Therefore, the present invention effectively overcomes the various shortcomings of the existing technology and has high industrial utilization value.

[0074] Example 2

[0075] In the examples of the present application, tetrahydrofuran (Aladdin, CAS No. 109-99-9) is used as the dispersant, and polycaprolactone (Sigma, 440744, USA) with a number average molecular weight of 6000 is used as the high molecular organic polymer.

[0076] The present invention provides a method for preparing a quercetin / polycaprolactone fiber electrospun membrane, comprising the following steps:

[0077] A) 0.05 g of quercetin powder (Sigma, Q4951, USA) was weighed and added to 10 ml of tetrahydrofuran. The mixture was shaken in an ultrasonic oscillator for 30 min to obtain a quercetin dispersion.

[0078] B) adding 2.0 g of polycaprolactone particles (PCL) to the quercetin dispersion and stirring on a magnetic stirrer for 12 h to obtain a spinnable solution;

[0079] C) At 25°C and 30% humidity, the spinnable solution was transferred to a 10 ml syringe with a 21G needle. A flow pump was used to control the solution propulsion rate at 1.5 ml / h. The spinning voltage was 12 kV, and the spinning time was 1 h. A drum with an 8 cm diameter was used as a collection device. The positive and negative voltages were +6 kV and -6 kV, respectively. The collection distance was 23 cm, and the reciprocating distance was 18 cm. After the spinning was completed, the electrospun membrane was removed from the drum and placed in a fume hood for 3 days to allow the organic solvent tetrahydrofuran to fully evaporate, thereby obtaining a quercetin / polycaprolactone fiber electrospun membrane.

[0080] Example 3

[0081] In the embodiments of the present application, the dispersant is a mixture of tetrahydrofuran (Aladdin, CAS No. 109-99-9) and toluene (Aladdin, CAS No. 90292-62-9) in a mass ratio of 1:1, and the high molecular organic polymer is polycaprolactone (Sigma, 440744, USA) with a number average molecular weight of 6000.

[0082] The present invention provides a method for preparing a quercetin / polycaprolactone fiber electrospun membrane, comprising the following steps:

[0083] A) Weigh 0.08 g of quercetin powder (Sigma, Q4951, USA) and add it to 10 ml of dispersant. Vibrate in an ultrasonic oscillator for 30 min to obtain a quercetin dispersion.

[0084] B) adding 1.0 g of polycaprolactone particles (PCL) to the quercetin dispersion and stirring on a magnetic stirrer for 12 h to obtain a spinnable solution;

[0085] C) At 30°C and 35% humidity, the spinnable solution was transferred to a 10 ml syringe with a 21G needle. A flow pump was used to control the solution propulsion rate at 1.5 ml / h. The spinning voltage was 15 kV, and the spinning time was 1 h. A drum with a diameter of 8 cm was used as a collection device. The positive and negative voltages were +7 kV and -8 kV, respectively. The collection distance was 25 cm, and the reciprocating distance was 15 cm. After the spinning was completed, the electrospun membrane was removed from the drum and placed in a fume hood for 3 days to allow the dispersant to fully evaporate, thereby obtaining a quercetin / polycaprolactone fiber electrospun membrane.

[0086] Example 4

[0087] In the embodiment of the present application, the dispersant is hexafluoroisopropanol (Macklin, H811026, Shanghai), and the high molecular organic polymer is polycaprolactone (Sigma, 440744, USA) with a number average molecular weight of 8000. The TEM image thereof is shown in FIG. Figure 2 shown.

[0088] The present invention provides a method for preparing a quercetin / polycaprolactone fiber electrospun membrane, comprising the following steps:

[0089] A) 0.064 g of quercetin powder (Sigma, Q4951, USA) was weighed and added to 10 ml of hexafluoroisopropanol (HFIP). The mixture was shaken in an ultrasonic oscillator for 30 min to obtain a quercetin dispersion.

[0090] B) adding 1.6 g of polycaprolactone particles (PCL) to the quercetin dispersion and stirring on a magnetic stirrer for 12 h to obtain a spinnable solution;

[0091] C) At 20°C and 40% humidity, the spinnable solution was transferred to a 10 ml syringe with a 21G needle. A flow pump was used to control the solution propulsion rate at 1.5 ml / h. The spinning voltage was 14 kV, and the spinning time was 2 h. A drum with a diameter of 8 cm was used as a collection device. The positive and negative voltages were +9 kV and -7 kV, respectively. The collection distance was 20 cm, and the reciprocating distance was 16 cm. After the spinning was completed, the electrospun membrane was removed from the drum and placed in a fume hood for 2 days to allow the organic solvent HFIP to fully evaporate, resulting in a quercetin / polycaprolactone fiber electrospun membrane with a thickness of 54 μm.

[0092] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the present invention. Anyone skilled in the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by one of ordinary skill in the art without departing from the spirit and technical principles disclosed herein are intended to be covered by the claims of the present invention.

Claims

1. Use of a quercetin / polycaprolactone fiber electrospun membrane in promoting osteogenic differentiation of hPDLSCs human periodontal ligament stem cells, characterized in that: The quercetin / polycaprolactone fiber electrospun membrane contains quercetin and polycaprolactone, and the mass ratio of the quercetin to the polycaprolactone is (5-8): (100-200); the thickness of the quercetin / polycaprolactone fiber electrospun membrane is 35-70 μm; the diameter of the fiber in the quercetin / polycaprolactone fiber electrospun membrane is 1.5-2.5 μm; the model of the polycaprolactone is Sigma 440744; the tensile strength of the quercetin / polycaprolactone fiber electrospun membrane is 2.3-3.3 MPa; The preparation method of the quercetin / polycaprolactone fiber electrospun membrane comprises the following steps: 1) dispersing quercetin powder and polycaprolactone in a dispersant and mixing them uniformly to obtain a spinnable solution; 2) preparing the quercetin / polycaprolactone fiber electrospun membrane by an electrospinning process using the spinnable solution; The amount of the quercetin powder added to the dispersant is 0.005-0.008 g / mL; the amount of the polycaprolactone added to the dispersant is 0.10-0.20 g / mL.

2. The use according to claim 1, characterized in that: The dispersant is selected from one or more of hexafluoroisopropanol, tetrahydrofuran and toluene.

3. The use according to claim 1, characterized in that: The conditions of the electrospinning process are: The feed rate of the spinnable solution is 1-1.5 mL / h; and / or, the electrospinning time is 1 to 3 h; and / or, using a drum as a collecting device with a collection speed of 8 to 12 r / min; And / or, in the electrospinning, the spinning voltage is 12-25 kV, the collection distance is 20-25 cm, and the reciprocating distance is 15-20 cm.

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