A method for simultaneously detecting isorotundic acid and rosmarinic acid in prunella vulgaris L. seeds and application thereof

Abstract

This invention discloses a method for simultaneously detecting isorhamnetin and rosmarinic acid in Prunella vulgaris seeds and its application. The method includes simultaneous qualitative identification of isorhamnetin and rosmarinic acid in Prunella vulgaris seeds using thin-layer chromatography; direct extraction of isorhamnetin and rosmarinic acid using a specific polar solvent extraction method and separation from lipid-soluble components; comparison with a mixed standard of isorhamnetin and rosmarinic acid; and simultaneous determination of the content of isorhamnetin and rosmarinic acid in Prunella vulgaris seeds using high-performance liquid chromatography. This method has advantages such as high accuracy and precision, good stability and repeatability, providing a method for quality detection of isorhamnetin and rosmarinic acid components in the development and utilization of Prunella vulgaris seeds, with strong specificity and good reproducibility.

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