Compositions with soothing, repairing and anti-wrinkle efficacy and use thereof

Abstract

The present application relates to a kind of compositions with soothing repair anti-wrinkle efficacy, consisting of the following components: 1-10 parts of ginsenoside, 90-99 parts of notoginseng stem and leaf extract;Wherein, the notoginseng stem and leaf extract includes the following weight ratio of components: 2-8:1 water-soluble organic solvent and notoginseng stem and leaf extract.The ginsenoside in the composition of the present application is compounded with notoginseng stem and leaf extract, has synergistic effect, and jointly plays the role of soothing repair anti-wrinkle.

Description

Technical Field

[0001] This invention relates to the field of skincare technology, and in particular to a composition with soothing, repairing and anti-wrinkle effects and its application. Background Technology

[0002] Ultraviolet radiation, blue light, environmental pollution, stress, and aging, coupled with inappropriate skincare, are causing internal and external adverse factors to affect human skin, resulting in a series of inflammatory responses. Initially, the skin exhibits clinical symptoms such as redness, stinging, and itching. With repeated stimulation, the stratum corneum thins further, and collagen hydrolysis in the basal layer accelerates, making the skin prone to dryness, sensitivity, roughness, pigmentation, and wrinkles. Therefore, inflammation is closely related to skin sensitivity, skin barrier function, and skin aging; skin problems are often accompanied by a variety of clinical symptoms.

[0003] Existing technologies mostly achieve cosmetics with single effects such as soothing, repairing, or anti-wrinkle by adding small molecule peptides, plant extracts, and small molecule compounds to traditional cosmetic formulations. To achieve multiple effects, it is necessary to add multiple or even more than a dozen active ingredients in combination. Although plant extracts are gentler and less irritating to the skin than chemical substances, and have more obvious advantages, they still face the problem of heavy metal and pesticide residues. Combining multiple extracts can easily lead to products with darker colors and more difficult-to-mask odors, and can also result in cosmetic products exceeding the standards for heavy metals and pesticide residues.

[0004] Patent application CN 112891243 A discloses an anti-wrinkle composition and its application, comprising ginsenosides, deer antler peptides, resveratrol, hydrolyzed collagen, sodium hyaluronate, superoxide dismutase, and honey. It exhibits good protective effects against oxidative damage to human immortalized keratinocytes and human dermal fibroblasts caused by ultraviolet radiation. It can promote cell growth, reduce matrix metalloproteinase and malondialdehyde secretion levels, and increase glutathione peroxidase secretion levels, demonstrating significant anti-wrinkle and anti-aging effects. However, it does not have soothing or repairing effects on irritated skin.

[0005] Patent CN201910522802.1 discloses a multi-effect repairing polypeptide composition and its application in cosmetics, comprising oligopeptide-1, carnosine, copper peptide, palmitoyl tetrapeptide-7, acetyl hexapeptide-8, and nonapeptide-1. This invention adds six polypeptide compositions to cosmetics, enabling the resulting cosmetics to have comprehensive repairing effects such as moisturizing, anti-oxidation, fading dark spots, anti-wrinkle, and soothing sensitive irritation, enhancing skin radiance and firmness, and restoring the skin to a healthy state. However, the polypeptides used are synthetic compounds, and impurities are easily introduced during the synthesis process, potentially introducing ingredients with side effects. Summary of the Invention

[0006] To overcome the shortcomings of existing products and address a range of skin problems caused by internal and external adverse factors, this invention provides a composition with soothing, repairing, and anti-wrinkle effects, as well as its applications. The composition provided by this invention can synergistically enhance each other, effectively relieving skin irritation, reducing hemoglobin levels in irritated skin conditions, soothing and improving skin irritation, and repairing the skin barrier; simultaneously, it diminishes wrinkles, exhibiting significant anti-wrinkle effects, and is gentle, safe, and non-irritating to the human body.

[0007] The objective of this invention is achieved through the following technical solution:

[0008] A composition with soothing, repairing and anti-wrinkle effects, comprising the following components: 1-10 parts of ginsenosides and 90-99 parts of Panax notoginseng stem and leaf extract; wherein the Panax notoginseng stem and leaf extract comprises the following components in the following weight ratio: 2-8:1 water-soluble organic solvent and Panax notoginseng stem and leaf extract.

[0009] Preferably, the composition comprises the following components: 2-7 parts of ginsenosides and 93-98 parts of Panax notoginseng stem and leaf extract.

[0010] Preferably, the water-soluble organic solvent is glycerol or butanediol.

[0011] Preferably, the Panax notoginseng stem and leaf extract comprises the following components in a weight ratio of 3-6:1: glycerol and Panax notoginseng stem and leaf extract.

[0012] Preferably, the Panax notoginseng stem and leaf extract comprises the following components in a weight ratio of 3-5:1: butanediol and Panax notoginseng stem and leaf extract.

[0013] Preferably, the ginsenosides are a mixture comprising ginsenosides 20-(R)-Rg3, 20-(S)-Rg3, Rg5, Rk1, 20-(R)-Rh2, 20-(S)-Rh2, Rh3, and Rk2.

[0014] Preferably, the Panax notoginseng stem and leaf extract is prepared by the following method:

[0015] Cut the stems and leaves of Panax notoginseng into sections, decoct them in water at least twice, filter the decoction, combine the filtrates and pass them through two tandem macroporous adsorption resin columns of type D101 or AB8, wash with 8-10 column volumes of water until colorless, then elute with 3-5 column volumes of 40%-95% ethanol, and pass the eluent through two tandem macroporous adsorption resin columns of type D280 or D900, collect the eluent, concentrate it to a thin extract, add glycerol or butylene glycol, and stir well to obtain the extract of Panax notoginseng stems and leaves.

[0016] Preferably, the Panax notoginseng stem and leaf extract is prepared by the following method:

[0017] The extract of Panax notoginseng stems and leaves is used as raw material, dissolved in 3-5 times the amount of deionized water, passed through a D900 macroporous adsorption resin, the eluent is collected, concentrated to a thin paste, and then glycerol or butylene glycol is added and stirred evenly to obtain the extract of Panax notoginseng stems and leaves.

[0018] The application of a composition with soothing, repairing and anti-wrinkle effects in the preparation of soothing, repairing and anti-wrinkle cosmetics, wherein the soothing and repairing composition is used in the preparation of cosmetics or skin care products, including lotions, creams, toners, masks and sprays.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] This invention relates to a compound containing ginsenosides and extracts from the stems and leaves of Panax notoginseng, forming an effective compound. The ginsenosides include eight rare ginsenosides: 20-(R)-Rg3, 20-(S)-Rg3, Rg5, Rk1, 20-(R)-Rh2, 20-(S)-Rh2, Rh3, and Rk2. Compared to polysaccharide-based ginsenosides such as Rb1, Rb2, Rb3, Rc, and Rd found in ginseng plants, these rare ginsenosides have a more significant effect on promoting collagen secretion by skin fibroblasts and exhibit more pronounced anti-inflammatory properties. On the other hand, the refined extract of Panax notoginseng stem and leaf extract obtained by the present invention through multi-step decolorization and impurity removal has a light color, removes most of the special odor, and enriches the active ingredient protopanaxadiol total saponins (Rb1, Rb2, Rb3, Rc, Rd, Fe) in Panax notoginseng stem and leaf. At the same time, by adding water-soluble organic solvents such as glycerol or butylene glycol to dissolve the Panax notoginseng stem and leaf extract, the solubility of the Panax notoginseng stem and leaf extract in water is improved. After being compounded with ginsenosides, the two have a synergistic effect, which has a wide range of skin protection effects and plays a role in soothing, repairing and anti-wrinkle. Detailed Implementation

[0021] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0022] Examples and Comparative Examples

[0023] This composition has soothing, repairing, and anti-wrinkle effects. See Table 1 for the specific composition.

[0024] Table 1

[0025] Ginsenosides Panax notoginseng stem and leaf extract Example 1 3.5 parts ginsenosides 1 96.5 portions of Panax notoginseng stem and leaf extract 1 Example 2 5 parts ginsenoside 1 95 portions of Panax notoginseng stem and leaf extract 2 Example 3 1 part ginsenoside 1 99 portions of Panax notoginseng stem and leaf extract 3 Example 4 5 parts ginsenoside 1 95 portions of Panax notoginseng stem and leaf extract 4 Comparative Example 1 5 parts ginsenoside 1 -- Comparative Example 2 -- 95 portions of Panax notoginseng stem and leaf extract 1 Comparative Example 3 5 parts ginsenosides 1' 95 portions of Panax notoginseng stem and leaf extract 1' Comparative Example 4 5 parts ginsenosides 1' 95 portions of Panax notoginseng stem and leaf extract 2' Comparative Example 5 5 parts ginsenoside 2' 95 portions of Panax notoginseng stem and leaf extract 3' Comparative Example 6 5 parts ginsenoside 2' 95 portions of Panax notoginseng stem and leaf extract 1

[0026] The specific amounts of Panax notoginseng stem and leaf extract and ginsenosides listed in the table above are as follows:

[0027] Ginsenoside 1 is a ginsenoside RGI from Jiangxi Kangyi Ginseng Biotechnology Co., Ltd. This ginsenoside is composed of more than 8 rare ginsenosides, including 20-(R)-Rg3, 20-(S)-Rg3, Rg5, Rk1, 20-(R)-Rh2, 20-(S)-Rh2, Rh3, and Rk2.

[0028] Ginsenoside 1' is a mixture of polysaccharide-based ginsenosides (Rb1, Rb2, Rb3, Rc, Rd, etc.).

[0029] Ginsenoside 2' is a mixture of total saponins from commercially available ginseng stems and leaves (Re, Rg1, Rb1, Rb2, Rb3, Rc, Rd, etc.).

[0030] Panax notoginseng stem and leaf extract 1 is prepared by the following method: Take an appropriate amount of Panax notoginseng stems and leaves, cut them into 2-3 cm sections, add water and decoct twice, the first time for 2 hours and the second time for 1.5 hours. Filter the decoction, combine the filtrates, filter the decoction again, and load the combined filtrates onto two tandem macroporous adsorption resin columns of type D101 or AB8. Elute with 8-10 column volumes of water until colorless, then elute with 3-5 column volumes of 40%-95% ethanol. Load the eluent onto two tandem macroporous adsorption resin columns of type D280 or D900. Collect the eluent, concentrate it to a thin paste, add about 3 times the weight of glycerol, and stir evenly to obtain Panax notoginseng stem and leaf extract 1.

[0031] The Panax notoginseng stem and leaf extract 2 is prepared by the following method: Take an appropriate amount of Panax notoginseng stems and leaves, cut them into 2-3 cm sections, add water and decoct twice, the first time for 2 hours and the second time for 1.5 hours. Filter the decoction, combine the filtrates, filter the decoction again, and load the combined filtrates onto two tandem macroporous adsorption resin columns of type D101 or AB8. Elute with 8-10 column volumes of water until colorless, then elute with 3-5 column volumes of 40%-95% ethanol. Load the eluent onto two tandem macroporous adsorption resin columns of type D280 or D900, collect the eluent, concentrate it to a thin paste, add about 4 times the weight of butanediol, and stir evenly to obtain Panax notoginseng stem and leaf extract 2.

[0032] Panax notoginseng stem and leaf extract 3 is prepared by the following method: commercially available Panax notoginseng stem and leaf extract powder (total saponins of Panax notoginseng stem and leaf ≥70%) is dissolved in 4-10 weight volumes of water. The aqueous solution is loaded onto a D101 or AB8 macroporous adsorption resin column and eluted with 8-10 column volumes of water until colorless. Then, it is eluted with 3-5 column volumes of 40%-95% ethanol. The eluent is then loaded onto a D280 or D900 macroporous adsorption resin. The eluent is collected, concentrated to a thin paste, and about 4 times its weight of glycerol is added. The mixture is stirred evenly to obtain Panax notoginseng stem and leaf extract 3.

[0033] Panax notoginseng stem and leaf extract 4 is prepared by the following method: commercially available Panax notoginseng stem and leaf extract powder (total saponins of Panax notoginseng stem and leaf ≥70%) is dissolved in 4-10 weight volumes of water. The aqueous solution is loaded onto a D101 or AB8 macroporous adsorption resin column and eluted with 8-10 column volumes of water until colorless. Then, it is eluted with 3-5 column volumes of 40%-95% ethanol. The eluent is then loaded onto a D280 or D900 macroporous adsorption resin. The eluent is collected, concentrated to a thin paste, and about 3 times its weight of butanediol is added. The mixture is stirred evenly to obtain Panax notoginseng stem and leaf extract 4.

[0034] Panax notoginseng stem and leaf extract 1' was prepared by the following method: a commercially available Panax notoginseng stem and leaf extract solution was dissolved in about 4 times its weight of butanediol and an appropriate amount of water to obtain Panax notoginseng stem and leaf extract 1'.

[0035] The Panax notoginseng stem and leaf extract 2' was prepared by the following method: cut Panax notoginseng stems and leaves into 2-3cm sections, add water and decoct twice, the first time for 2 hours and the second time for 1.5 hours. Filter the decoction, combine the filtrates, add 95% ethanol to prepare a 75% ethanol solution, let it stand to precipitate, filter, concentrate the filtrate to a thin extract, add about 4 times the weight of glycerol, and stir well to obtain Panax notoginseng stem and leaf extract 2'.

[0036] Panax notoginseng stem and leaf extract 3' is a commercially available Panax notoginseng stem and leaf extract, with approximately 3 times its weight of butylene glycol added to prepare an aqueous solution of Panax notoginseng stem and leaf extract 3'.

[0037] Application test results

[0038] The compositions of Examples 1-4 and Comparative Examples 1-6 were used as active ingredients in the preparation of serums, with an addition amount of 6%.

[0039] The specific formula of the essence lotion is as follows: 3.71% butylene glycol, 7% sorbitol (70% sugar solution), 4% caprylic / capric triglycerides, 3% shea butter (BUTYROSPERMUM PARKII), 3% squalane, 2% alkyl glycoside emulsifier, 0.5% 1,2-hexanediol, 0.5% p-hydroxyacetophenone, 0.5% trehalose, 0.3% carbomer, 0.3% arginine, 0.05% disodium EDTA, 0.03% sodium hyaluronate, 0.02% xanthan gum, 0.02% pentaerythritol tetra(bis-tert-butylhydroxyhydrocinnamic acid) ester, 0.02% (daily use) fragrance, and the balance being water.

[0040] The preparation method of the above-mentioned multi-functional essence lotion includes the following steps:

[0041] (1) Add some butanediol, sodium hyaluronate and xanthan gum to a container and stir until completely mixed. Set aside.

[0042] (2) Add some water and arginine raw materials to a container, stir and mix until completely dissolved and uniform, and set aside.

[0043] (3) Add some water, some butanediol and p-hydroxyacetophenone raw materials to a container, heat to 60-65℃ and stir until completely dissolved and homogeneous, then set aside.

[0044] (4) Add some water and carbomer to the main pot and stir to homogenize the materials until they are completely mixed.

[0045] (5) Add the mixed raw materials, sorbitol, 1,2-hexanediol, trehalose, ginsenosides and disodium EDTA into the main pot and heat to 80-85℃. Stir to make the materials completely and evenly mixed.

[0046] (6) Add the raw materials caprylic / capric triglyceride, BUTYROSPERMUM PARKII fruit oil, squalane, alkyl glycoside emulsifier and pentaerythritol tetra(bis-tert-butylhydroxyhydrocinnamic acid) ester to the oil phase pot, stir and heat to 85-90℃ to make the materials completely and evenly mixed, add to the main pot, stir and homogenize until completely and evenly mixed.

[0047] (7) Cool the main pot to 60-65℃, then add the mixed raw materials 2 and 3 to the main pot in sequence, stir until homogenized and fully mixed, and adjust the pH of the material.

[0048] (8) Continue to cool the main pot temperature to below 40°C, then add the Panax notoginseng stem and leaf extract and (daily use) fragrance raw materials to the main pot and stir until completely mixed.

[0049] (9) After the material is tested and qualified, it can be filled, packaged, and the finished product can be put into the warehouse only after it is tested and qualified.

[0050] 2. The efficacy of the serums prepared in each example is verified as follows:

[0051] Subject population

[0052] Number of genders: male / female, 8 people per sample;

[0053] Age distribution: 18 to 60 years old;

[0054] Health status: Subjects have healthy skin, no history of skin diseases or allergies, and meet the criteria for voluntary inclusion in the subject selection process.

[0055] Grouping: Efficacy evaluation used serum samples from Examples 1-4 and Comparative Examples 1-6, respectively.

[0056] Test Method: Subjects cleaned their faces with dry paper towels and sat still for 30 minutes in a laboratory at 21℃±1℃ and 50%±10%RH. Tests were then performed on the subjects' faces to measure transepidermal water loss (TEWL), dermal hemoglobin (Erythema), and skin wrinkle index (SEw) before and 28 days after sample use. The same area of ​​the subjects' cheeks was tested using an MPA580 skin elasticity tester (Corneometer probe, Tewameter probe, Mexameter probe) and a Visioscan VC20plus skin texture analysis system. The arithmetic mean was taken, and the results are shown in Tables 2, 3, 4, and 5.

[0057] Table 2 Transepidermal Water Loss (TEWL) Values

[0058] sample Base value (D0) 28 days after using the sample (D28) Example 1 23.42±5.41 17.53±5.43 Example 2 22.45±5.01 16.65±4.75 Example 3 24.76±4.67 17.23±5.87 Example 4 23.19±5.44 15.96±6.02 Compare with Example 1 24.01±6.89 20.97±5.45 Compare with Example 2 23.48+5.78 20.27±5.19 Compare with Example 3 23.17+4.96 19.23±6.45 Compare with Example 4 24.12±5.23 20.13±4.97 Compare with Example 5 22.32±4.78 19.34±5.65 Compare with Example 6 23.78±6.02 20.12±4.56

[0059] Table 3. Erythema values ​​of skin hemoglobin

[0060] sample Base value (D0) 28 days after using the sample (D28) Example 1 298.23±56.78 258.45±45.34 Example 2 311.09±60.12 265.67±58.15 Example 3 302.67±59.43 253.03±60.67 Example 4 310.75±62.22 254.69±60.12 Compare with Example 1 304.73±59.68 283.44±61.64 Compare with Example 2 302.34±60.35 278.13±61.39 Compare with Example 3 312.42±60.32 280.84±59.56 Compare with Example 4 306.18±61.75 273.34±59.98 Compare with Example 5 307.49±58.78 273.34±60.12 Compare with Example 6 311.34±60.34 279.23±58.12

[0061] Table 4. Wrinkle Index (Sew Value)

[0062] sample Base value (D0) 28 days after using the sample (D28) Example 1 67.18±15.78 56.39±14.98 Example 2 65.96±15.02 55.67±17.51 Example 3 64.67±15.54 55.78±15.93 Example 4 65.25±16.21 57.01±16.76 Compare with Example 1 64.25±16.28 61.23±15.79 Compare with Example 2 68.79±17.65 64.12±15.31 Compare with Example 3 63.87±14.68 61.13±15.73 Compare with Example 4 65.24±16.01 62.78±16.21 Compare with Example 5 66.43±14.92 63.32±15.65 Compare with Example 6 64.15±14.76 60.09±16.54

[0063] Lactic acid stinging test: Clean the subject's face with a dry paper towel. Place 50 μl of 10% lactic acid on a single layer of filter paper with a diameter of 0.8 cm and place it on the subject's nasolabial fold. Ask the subject about stinging sensation and observe the skin reaction at 30 seconds, 2.5 minutes, and 5 minutes. Score the stinging sensation from 0 to 3 points, indicating no stinging, mild stinging, moderate stinging, and severe stinging. A total score of ≥3 points in the lactic acid stinging test is required to be selected as a subject with sensitive skin. The results of the lactic acid stinging test scores are shown in Table 5.

[0064] Table 5 Lactic acid stinging scores

[0065] sample Base value (D0) 28 days after using the sample (D28) Example 1 4.42±0.87 1.58±0.60 Example 2 4.31±0.76 1.49±0.75 Example 3 4.43±0.62 1.44±0.89 Example 4 4.03±0.56 1.24±0.72 Compare with Example 1 4.86±0.66 1.98±0.54 Compare with Example 2 4.09±0.57 2.1±0.83 Compare with Example 3 3.78±0.46 1.87±0.34 Compare with Example 4 4.25±0.78 2.1±0.53 Compare with Example 5 4.03±0.63 1.98±0.77 Compare with Example 6 4.69±0.82 2.31±0.69

[0066] As shown in Tables 2-5, the products in the examples significantly improved transdermal water loss (TEWL) values, skin hemoglobin (Erythema) values, wrinkle index (Sew) values, and lactic acid stinging scores compared to the products in the comparative examples. Because the pigments and odors from the Panax notoginseng stem and leaf extract added to the comparative examples were not completely removed, the products had a yellow to light brown appearance and a strong odor, which was difficult for participating users to accept.

[0067] The above description of the embodiments is provided to enable those skilled in the art to understand and use the invention. It will be apparent to those skilled in the art that various modifications can be made to these embodiments, and the general principles described herein can be applied to other embodiments without inventive effort. Therefore, the present invention is not limited to the above embodiments, and any improvements and modifications made by those skilled in the art based on the disclosure of the present invention without departing from the scope of the invention should be within the protection scope of the present invention.

Claims

1. A composition with soothing, repairing, and anti-wrinkle effects, characterized in that, It consists of the following components: 3.5 parts ginsenosides and 96.5 parts Panax notoginseng stem and leaf extract; The ginsenosides mentioned therein are selected from ginsenoside RGI of Jiangxi Kangyi Ginseng Biotechnology Co., Ltd., and the ginsenosides are composed of rare ginsenosides such as 20-(R)-Rg3, 20-(S)-Rg3, Rg5, Rk1, 20-(R)-Rh2, 20-(S)-Rh2, Rh3, and Rk2. The Panax notoginseng stem and leaf extract is prepared by the following method: Take an appropriate amount of Panax notoginseng stems and leaves, cut them into 2-3 cm sections, add water and decoct twice, the first time for 2 hours and the second time for 1.5 hours. Filter the decoction, combine the filtrates and pass them through two tandem macroporous adsorption resin columns of type D101 or AB8. Elute with 8-10 column volumes of water until colorless, then elute with 3-5 column volumes of 40%-95% ethanol. Pass the eluent through two tandem macroporous adsorption resin columns of type D280 or D900. Collect the eluent, concentrate it to a thin paste, add 3 times the weight of glycerol, and stir evenly to obtain the Panax notoginseng stem and leaf extract.

2. A composition with soothing, repairing, and anti-wrinkle effects, characterized in that, It consists of the following components: 5 parts ginsenosides and 95 parts Panax notoginseng stem and leaf extract; The ginsenosides mentioned therein are selected from ginsenoside RGI of Jiangxi Kangyi Ginseng Biotechnology Co., Ltd., and the ginsenosides are composed of rare ginsenosides such as 20-(R)-Rg3, 20-(S)-Rg3, Rg5, Rk1, 20-(R)-Rh2, 20-(S)-Rh2, Rh3, and Rk2. The Panax notoginseng stem and leaf extract is prepared by the following method: Take an appropriate amount of Panax notoginseng stems and leaves, cut them into 2-3 cm sections, add water and decoct twice, the first time for 2 hours and the second time for 1.5 hours. Filter the decoction, combine the filtrates and pass them through two tandem macroporous adsorption resin columns of type D101 or AB8. Elute with 8-10 column volumes of water until colorless, then elute with 3-5 column volumes of 40%-95% ethanol. Pass the eluent through two tandem macroporous adsorption resin columns of type D280 or D900. Collect the eluent, concentrate it to a thin paste, add 4 times the weight of butanediol, and stir evenly to obtain the Panax notoginseng stem and leaf extract.

3. A composition with soothing, repairing, and anti-wrinkle effects, characterized in that, It consists of the following components: 1 part ginsenosides and 99 parts Panax notoginseng stem and leaf extract; The ginsenosides mentioned therein are selected from ginsenoside RGI of Jiangxi Kangyi Ginseng Biotechnology Co., Ltd., and the ginsenosides are composed of rare ginsenosides such as 20-(R)-Rg3, 20-(S)-Rg3, Rg5, Rk1, 20-(R)-Rh2, 20-(S)-Rh2, Rh3, and Rk2. The Panax notoginseng stem and leaf extract was prepared by the following method: commercially available Panax notoginseng stem and leaf extract powder with a total saponin content of more than 70% was dissolved in 4-10 times its volume of water. The aqueous solution was passed through a D101 or AB8 macroporous adsorption resin column and eluted with 8-10 column volumes of water until colorless. Then, it was eluted with 3-5 column volumes of 40%-95% ethanol. The eluent was then passed through a D280 or D900 macroporous adsorption resin. The eluent was collected, concentrated to a thin paste, and 4 times its weight of glycerol was added. The mixture was stirred evenly to obtain the Panax notoginseng stem and leaf extract.

4. A composition with soothing, repairing, and anti-wrinkle effects, characterized in that, It consists of the following components: 5 parts ginsenosides and 95 parts Panax notoginseng stem and leaf extract; The ginsenosides mentioned therein are selected from ginsenoside RGI of Jiangxi Kangyi Ginseng Biotechnology Co., Ltd., and the ginsenosides are composed of rare ginsenosides such as 20-(R)-Rg3, 20-(S)-Rg3, Rg5, Rk1, 20-(R)-Rh2, 20-(S)-Rh2, Rh3, and Rk2. The Panax notoginseng stem and leaf extract was prepared by the following method: commercially available Panax notoginseng stem and leaf extract powder with a total saponin content of more than 70% was dissolved in 4-10 times its volume of water. The aqueous solution was passed through a D101 or AB8 macroporous adsorption resin column and eluted with 8-10 column volumes of water until colorless. Then, it was eluted with 3-5 column volumes of 40%-95% ethanol. The eluent was then passed through a D280 or D900 macroporous adsorption resin. The eluent was collected, concentrated to a thin paste, and 3 times its weight of butylene glycol was added. The mixture was stirred evenly to obtain the Panax notoginseng stem and leaf extract.

5. The use of a composition with soothing, repairing and anti-wrinkle effects as described in any one of claims 1-4 in the preparation of soothing, repairing and anti-wrinkle cosmetics.