A scaffold material for breast surgery and a method of making the same
The core-shell scaffold material prepared by 3D printing, with the addition of nano-sized lanthanum and cross-linking treatment, solves the problems of insufficient compressive strength and insufficient biocompatibility of existing scaffold materials, and achieves a balance between improved compressive strength and biocompatibility, supporting cell growth in breast surgery.
Patent Information
- Application Number
- CN202310837747.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2023-07-10
- Publication Date
- 2025-11-25
- Estimated Expiration
- 2043-07-10
AI Technical Summary
The physical properties (such as compressive strength) of existing breast surgery stent materials are not satisfactory, and their biocompatibility is insufficient, which affects cell proliferation and adhesion.
Core-shell scaffold materials were prepared using 3D printing technology. Dopamine/sodium alginate was used as the shell, and lanthanum nanoparticles were added. The compressive strength of the scaffold material was improved by cross-linking treatment, and the content of lanthanum nanoparticles was controlled to maintain biocompatibility.
It significantly improves the compressive strength of the scaffold material, while maintaining good biocompatibility with the addition of an appropriate amount of nano-metallic lanthanum, providing a suitable three-dimensional environment to support cell growth.
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the field of medical stents, in particular to a stent material for breast surgery and a preparation method thereof. BACKGROUND
[0002] Breast is a special soft tissue, when the treatment of disease leads to the loss or deformity of breast, it will have a great impact on the quality of life and mental health of women. And the stent material, as one of the elements of tissue engineering, also has very important significance in breast tissue engineering. The stent material should have physical and chemical properties suitable for the target tissue, because these physical and chemical properties will affect the behavior of cell proliferation, differentiation and adhesion. The ideal stent material should have good biocompatibility, that is, low immunogenicity, in addition, it should also have certain mechanical properties to provide a suitable three-dimensional environment for the adhesion and growth of seed cells. In the prior art, some researchers use 3D printing technology to prepare a core-shell stent material, which takes gelatin as the core and polydopamine / sodium alginate as the shell, but its physical properties (such as compressive strength) are not satisfactory. SUMMARY
[0003] In view of the problems existing in the prior art, the present application aims to provide a preparation method of a stent material for breast surgery.
[0004] The preparation method of the stent material for breast surgery provided by the present application comprises the following steps:
[0005] 100-120mg of dopamine is dissolved in 25-30mL of Tris-HCl solution, then 4.0-4.5g of sodium alginate is added, and after stirring thoroughly, a paste is obtained. 7.5-12.3wt% of nano-metal lanthanum is added to the paste and stirred vigorously to disperse uniformly to obtain a printing ink.
[0006] The printing ink and 45-50wt% of gelatin aqueous solution are transferred to different printing tubes, and a coaxial core-shell needle is used to obtain a core-shell stent material with gelatin as the core by 3D printing, wherein the printing speed is 3.5-4mm / s, the pressure is 300-320kPa, the temperature of the printing ink is 25-28℃, and the temperature of the gelatin aqueous solution is 60-65℃. Finally, the stent material is placed in a 1.1-1.2M CaCl2 solution for crosslinking treatment for 10-15min, and then washed with deionized water several times after taking out.
[0007] Preferably, the concentration of the Tris-HCl solution is 10mM.
[0008] Preferably, the pH of the Tris-HCl solution is 8.5.
[0009] Preferably, the concentration of the CaCl2 solution is 1.2M.
[0010] Preferably, the time of the cross-linking treatment is 10min.
[0011] Preferably, the concentration of the gelatin aqueous solution is 50wt%.
[0012] Further, the present application also provides a scaffold material for breast surgery, which is prepared by the above method.
[0013] After adding nano-metal lanthanum into the dopamine / sodium alginate printing ink, the compressive strength of the scaffold material can be significantly improved, meanwhile, with the increase of the content of nano-metal lanthanum, the biocompatibility of the scaffold material presents a trend of first increasing and then decreasing, and too much use of nano-metal lanthanum does not obviously improve the compressive strength, but instead makes the biocompatibility of the scaffold material decrease sharply. DETAILED DESCRIPTION
[0014] The technical effects of the present application are verified by specific examples below, but the implementation of the present application is not limited thereto.
[0015] Example 1
[0016] 100mg dopamine was dissolved in 30mL Tris-HCl solution (concentration of 10mM, pH=8.5), then 4.0g sodium alginate was added, and after fully stirring, a paste was obtained. 7.5wt% nano-metal lanthanum was added to the paste and stirred vigorously to disperse uniformly to obtain a printing ink.
[0017] The printing ink and 50wt% gelatin aqueous solution were transferred to different printing tubes, and a core-shell scaffold material with gelatin as the core was obtained by 3D printing with coaxial core-shell needles, wherein the printing speed was 4mm / s, the pressure was 320kPa, the temperature of the printing ink was 28℃, and the temperature of the gelatin aqueous solution was 65℃. Finally, the scaffold material was placed in a 1.2M CaCl2 solution for cross-linking treatment for 10min, and then washed with deionized water several times after being taken out.
[0018] Example 2
[0019] 100mg dopamine was dissolved in 30mL Tris-HCl solution (concentration of 10mM, pH=8.5), then 4.0g sodium alginate was added, and after fully stirring, a paste was obtained. 9.6wt% nano-metal lanthanum was added to the paste and stirred vigorously to disperse uniformly to obtain a printing ink.
[0020] The printing ink and 50wt% gelatin aqueous solution were transferred to different printing tubes, and a coaxial core-shell needle was used to obtain a gelatin core-shell scaffold material by 3D printing, wherein the printing speed was 4 mm / s, the pressure was 320 kPa, the temperature of the printing ink was 28°C, and the temperature of the gelatin aqueous solution was 65°C. Finally, the scaffold material was placed in a 1.2M CaCl2 solution for crosslinking treatment for 10 min, and then washed with deionized water several times.
[0021] Example 3
[0022] 100 mg of dopamine was dissolved in 30 mL of Tris-HCl solution (concentration of 10 mM) with pH = 8.5, and then 4.0 g of sodium alginate was added. After stirring, a paste was obtained. 10.8wt% of nano-metal lanthanum was added to the paste and stirred vigorously to disperse uniformly to obtain a printing ink.
[0023] The printing ink and 50wt% gelatin aqueous solution were transferred to different printing tubes, and a coaxial core-shell needle was used to obtain a gelatin core-shell scaffold material by 3D printing, wherein the printing speed was 4 mm / s, the pressure was 320 kPa, the temperature of the printing ink was 28°C, and the temperature of the gelatin aqueous solution was 65°C. Finally, the scaffold material was placed in a 1.2M CaCl2 solution for crosslinking treatment for 10 min, and then washed with deionized water several times.
[0024] Example 4
[0025] 100 mg of dopamine was dissolved in 30 mL of Tris-HCl solution (concentration of 10 mM) with pH = 8.5, and then 4.0 g of sodium alginate was added. After stirring, a paste was obtained. 10.8wt% of nano-metal lanthanum was added to the paste and stirred vigorously to disperse uniformly to obtain a printing ink.
[0026] The printing ink and 50wt% gelatin aqueous solution were transferred to different printing tubes, and a coaxial core-shell needle was used to obtain a gelatin core-shell scaffold material by 3D printing, wherein the printing speed was 4 mm / s, the pressure was 320 kPa, the temperature of the printing ink was 28°C, and the temperature of the gelatin aqueous solution was 65°C. Finally, the scaffold material was placed in a 1.2M CaCl2 solution for crosslinking treatment for 10 min, and then washed with deionized water several times.
[0027] Example 5
[0028] Dopamine 100 mg was dissolved in 30 mL Tris-HCl solution (10 mM, pH = 8.5), and then 4.0 g sodium alginate was added. After stirring, a paste was obtained. 6.2 wt% of nano-metal lanthanum and 4.6 wt% of nano-metal cerium were added to the paste and stirred to disperse uniformly to obtain a printing ink.
[0029] The printing ink and 50 wt% of gelatin aqueous solution were transferred to different printing tubes, and a coaxial core-shell needle was used to obtain a gelatin core-shell scaffold material by 3D printing, wherein the printing speed was 4 mm / s, the pressure was 320 kPa, the temperature of the printing ink was 28°C, and the temperature of the gelatin aqueous solution was 65°C. Finally, the scaffold material was placed in a 1.2 M CaCl2 solution for crosslinking treatment for 10 min, and then washed with deionized water several times.
[0030] Comparative Example 1
[0031] Dopamine 100 mg was dissolved in 30 mL Tris-HCl solution (10 mM, pH = 8.5), and then 4.0 g sodium alginate was added. After stirring, a paste was obtained.
[0032] The printing ink and 50 wt% of gelatin aqueous solution were transferred to different printing tubes, and a coaxial core-shell needle was used to obtain a gelatin core-shell scaffold material by 3D printing, wherein the printing speed was 4 mm / s, the pressure was 320 kPa, the temperature of the printing ink was 28°C, and the temperature of the gelatin aqueous solution was 65°C. Finally, the scaffold material was placed in a 1.2 M CaCl2 solution for crosslinking treatment for 10 min, and then washed with deionized water several times.
[0033] Comparative Example 2
[0034] Dopamine 100 mg was dissolved in 30 mL Tris-HCl solution (10 mM, pH = 8.5), and then 4.0 g sodium alginate was added. After stirring, a paste was obtained. 20.0 wt% of nano-metal lanthanum was added to the paste and stirred to disperse uniformly to obtain a printing ink.
[0035] The printing ink and 50 wt% of gelatin aqueous solution were transferred to different printing tubes, and a coaxial core-shell needle was used to obtain a gelatin core-shell scaffold material by 3D printing, wherein the printing speed was 4 mm / s, the pressure was 320 kPa, the temperature of the printing ink was 28°C, and the temperature of the gelatin aqueous solution was 65°C. Finally, the scaffold material was placed in a 1.2 M CaCl2 solution for crosslinking treatment for 10 min, and then washed with deionized water several times.
[0036] In the following, the compressive strength and biocompatibility of the scaffold materials in Examples 1-5 and Comparative Examples 1-2 are evaluated, and the test results are shown in Table 1, wherein the test method of biocompatibility is as follows:
[0037] Biocompatibility: The biocompatibility of each sample is evaluated by a hemolysis test, which is based on the principle that the amount of hemoglobin released after the rupture of red blood cell membranes is measured to detect the in vitro hemolysis degree of each sample after the sample is directly contacted with blood. The absorption wavelength of hemoglobin is 545 nm, and the concentration thereof can be detected by a spectrophotometer. The specific operation steps are as follows:
[0038] (1) After a healthy rabbit is anesthetized and fixed, 4 mL of venous blood is slowly extracted from the rabbit's auricular vein, 0.2 mL of 2% potassium oxalate is added, and fresh anticoagulant blood is prepared. 5 mL of 0.9% sodium chloride injection is added for dilution.
[0039] (2) Three siliconized test tubes are taken, one test tube is filled with the test sample and 10 mL of sodium chloride injection, one test tube is blank and added with 10 mL of sodium chloride physiological saline as a negative control group, and the other test tube is blank and added with 10 mL of distilled water as a positive control group.
[0040] (3) All test tubes are kept at 37°C in a water bath for 30 min, 0.2 mL of anticoagulant rabbit blood is added, and the test tubes are kept at 37°C for 60 min, and then are put into a centrifuge at 1000 rpm for 5 min.
[0041] (4) The upper clear liquid of the test tube is taken, and the absorbance is measured at a wavelength of 545 nm. Each sample is tested in triplicate and the average value is taken.
[0042] The calculation formula of the hemolysis rate is as follows:
[0043] Hemolysis rate (%) = (average absorbance of test sample - absorbance of negative group) / (absorbance of positive group - absorbance of negative group) x 100
[0044] Table 1
[0045] No. Compressive strength / kPa Hemolysis rate / % Example 1 90.3 4.3 Example 2 94.6 3.9 Example 3 101.4 3.2 Example 4 100.9 5.7 Example 5 103.7 1.2 Comparative Example 1 82.1 5.1 Comparative Example 2 102.0 10.7
[0046] As can be seen from Table 1, the addition of nano-metal lanthanum to the dopamine / sodium alginate printing ink can significantly improve the compressive strength of the scaffold material, and at the same time, with the increase of the content of nano-metal lanthanum, the biocompatibility of the scaffold material shows a trend of first increasing and then decreasing, and too much use of nano-metal lanthanum does not obviously improve the compressive strength, but rather sharply decreases the biocompatibility of the scaffold material.
[0047] The above merely describes the preferred embodiments of the present application, and it should be pointed out that, for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present application, and these improvements and modifications should also be considered as the protection scope of the present application.
Claims
1. A method for preparing a scaffold material for breast surgery, characterized by, The method comprises the following steps: 100-120 mg of dopamine is dissolved in 25-30 mL of Tris-HCl solution, and then 4.0-4.5 g of sodium alginate is added, and after being fully stirred, a paste is obtained; 10.8-12.3 wt% of nano-metal lanthanum is added to the paste and stirred vigorously to disperse uniformly to obtain a printing ink; The printing ink and 45-50 wt% of a gelatin aqueous solution are transferred to different printing tubes, and a core-shell scaffold material with gelatin as the core is obtained by 3D printing through a coaxial core-shell needle, wherein the printing speed is 3.5-4 mm / s, the pressure is 300-320 kPa, the temperature of the printing ink is 25-28℃, and the temperature of the gelatin aqueous solution is 60-65℃; finally, the scaffold material is placed in a 1.1-1.2 M CaCl2 solution for crosslinking treatment for 10-15 min, and after being taken out, it is washed with deionized water for several times.
2. A process according to claim 1, wherein the process is carried out at a temperature of from 20 to 100°C. The concentration of the Tris-HCl solution is 10 mM.
3. A preparation method as described in claim 1, characterized in that, The pH of the Tris-HCl solution is 8.
5.
4. The production method as claimed in claim 1, characterized in that, The concentration of the CaCl2 solution is 1.2 M.
5. A preparation method as described in claim 1, characterized in that, The crosslinking treatment time is 10 min.
6. A preparation method as described in claim 1, characterized in that, The concentration of the gelatin aqueous solution is 50 wt%.
7. A scaffold material for breast surgery, characterized in that, The scaffold material is prepared by the method of any one of claims 1-6.