A method for identifying an extracellular vesicle
By using the adhesion and fluorescent labeling method of poly-L-lysine and fluorescein-5-maleimide solution, the problems of complexity and poor reproducibility in the identification of extracellular vesicles in the prior art are solved, and rapid and accurate detection of extracellular vesicles is achieved.
CN116773499BActive Publication Date: 2026-02-27SICHUAN UNIV
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Patent Information
- Application Number
- CN202310765107.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2023-06-26
- Publication Date
- 2026-02-27
- Estimated Expiration
- 2043-06-26
AI Technical Summary
Technical Problem
Existing methods for identifying extracellular vesicles are complex to operate and have poor reproducibility, making it difficult to accurately detect marker proteins in biological fluids.
Method used
Extracellular vesicles were labeled with polylysine solution and fluorescein-5-maleimide solution for adhesion and fluorescence, and then identified by confocal microscopy, achieving rapid and specific labeling.
Benefits of technology
It enables rapid and accurate detection of extracellular vesicles, and can complete the detection within 3 hours, improving detection efficiency and repeatability.
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Abstract
The application discloses a method for identifying extracellular vesicles, and relates to the technical field of identification of extracellular vesicles. The method comprises the following steps in sequence: dropping a polylysine solution on a glass slide, carrying out coating, air-drying, then adding an extracellular vesicle solution, incubating at room temperature, then adding a fluorescein-5-maleimide solution, incubating at room temperature in the dark, and then carrying out fluorescence identification under a confocal microscope. The method realizes rapid detection of the vesicles by using fluorescein-5-maleimide for marking detection.
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