Sapindus fermentation filtrate, preparation method thereof, and cosmetic

Abstract

The application relates to the field of cosmetics, in particular to a soapberry fermentation filtrate, a preparation method thereof and cosmetics. A preparation method of a soapberry fermentation filtrate, the method comprising the following steps: culturing and fermenting water-extracted soapberry residues and schizophyllum commune liquid in a culture medium, and taking a filtrate. In the application, the water-extracted soapberry residues and the schizophyllum commune liquid are cultured, the components which are not dissolved in water can be dissolved by biological fermentation, the soapberry fermentation filtrate can be used to develop cosmetics with the effects of improving skin barrier, removing yellow and brightening, and thus the utilization rate of soapberry is greatly improved, and the recyclability of resources is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics, in particular, to a soapberry fermentation filtrate, a preparation method thereof and a cosmetic. BACKGROUND

[0002] Soapberry is the seed of Sapindus officinalis of Sapindaceae. Soapberry has the effects of clearing heat, expelling phlegm, eliminating accumulation and killing insects. It is used for treating laryngalgia, lung heat cough, hoarseness, food retention, malnutrition, roundworm abdominal pain, trichomonas vaginitis, tinea and abscess.

[0003] At present, the utilization of soapberry needs to be improved. SUMMARY

[0004] The purpose of the embodiments of the present application is to provide a soapberry fermentation filtrate, a preparation method thereof and a cosmetic.

[0005] The first aspect of the present application provides a soapberry fermentation filtrate preparation method, which comprises:

[0006] providing soapberry water-extracted dregs;

[0007] The Schizophyllum commune liquid is treated by pulse electric field, and then cultured with the soapberry water-extracted dregs in a culture medium. Sodium chloride is added in the late culture stage for continuous culture.

[0008] Then, chitosan hydrochloride is added, stirred uniformly, centrifuged, filtered, and the filtrate is taken.

[0009] In the above technical solution of the present application, the soapberry water-extracted dregs are cultured with the Schizophyllum commune liquid, which can realize the use of biological fermentation technology to dissolve the components that are not dissolved in water by biological fermentation decomposition. This soapberry fermentation filtrate can be used to develop cosmetics with the effects of improving skin barrier, removing yellow and brightening, thereby greatly improving the utilization rate of soapberry and being conducive to improving resource recycling.

[0010] Further, the inventors found that the use of Schizophyllum commune for fermentation of soapberry water-extracted dregs can greatly improve the yield of active substances. Other strains generally have poor effects.

[0011] The preparation method of the sapindus fermenting filtrate provided in the application greatly improves the yield of the fermentation of the schizophyllum commune by treating the schizophyllum commune liquid with the pulsed electric field and culturing the sapindus water-extracted residue in the culture medium; adding sodium chloride in the middle and later stages of the culture can promote the fermentation efficiency of the schizophyllum commune in the middle and later stages, improve the output of the active substances, improve the activity of the schizophyllum commune, prevent the schizophyllum commune from aging due to excessive reproduction, and effectively ensure the fermentation efficiency; simultaneously adding chitosan hydrochloride can speed up the subsequent centrifugal filtration, because the liquid is viscous, it is difficult to handle in the production process, the addition of chitosan hydrochloride can quickly separate the bacteria from the fermentation liquid, and the subsequent filtration is more clear. The various steps of the preparation method provided in the application are synergistically matched, which greatly improves the content of the active substances in the sapindus water-extracted residue; and the obtained active substances have excellent effects of improving skin barrier, removing yellow and brightening.

[0012] In other embodiments of the application, the intensity of the pulsed electric field is 1KV / cm-4KV / cm, and the number of pulsed electric field cycle treatments is 20-60 times.

[0013] Some embodiments of the application provide a sapindus fermenting filtrate preparation method, which comprises:

[0014] The sapindus water-extracted residue and the schizophyllum commune liquid are cultured in the culture medium to ferment, and the filtrate is obtained.

[0015] In other embodiments of the application, the addition amount of the residue is 2%-8% of the volume of the culture medium; and the addition amount of the schizophyllum commune liquid is 5%-20% of the volume of the culture medium.

[0016] In other embodiments of the application, the concentration of the schizophyllum commune liquid in the schizophyllum commune liquid is 50%-80% by weight of the mycelium wet weight / liquid weight.

[0017] In other embodiments of the application, the culture fermentation comprises:

[0018] In the culture medium, the culture is carried out at 27-30°C for 1-6 days.

[0019] In other embodiments of the application, the culture fermentation comprises:

[0020] In the culture medium, after the culture is carried out at 27-30°C for 1-2 days, sodium chloride is added, and the culture is continued for 2-4 days; the addition amount of the sodium chloride is 5%-20% of the volume of the culture medium.

[0021] In other embodiments of the application, the obtaining of the filtrate comprises:

[0022] Chitosan hydrochloride is added to the fermentation product, mixed uniformly, centrifuged, filtered, and the filtrate is obtained; the addition amount of the chitosan hydrochloride is 2.0%-5.0% of the mass of the fermentation product.

[0023] In other embodiments of the present application, the Schizophyllum liquid is treated by pulsed electric field.

[0024] In other embodiments of the present application, the intensity of the pulsed electric field is 1KV / cm-4KV / cm, and the number of pulsed electric field cycle treatments is 20-60 times.

[0025] In other embodiments of the present application, the medicinal residue after water extraction of Sapindus is prepared by the following steps:

[0026] The Sapindus medicinal material is mixed with water, and extracted at 60-90℃ for 0.5-1.0h. After centrifugation, the medicinal residue is taken.

[0027] Optionally, the Sapindus medicinal material is mixed with water by the following steps:

[0028] The Sapindus medicinal material is mixed with water in a mass ratio of 1:(5-10).

[0029] In a second aspect, the present application provides a Sapindus fermentation filtrate prepared by the method for preparing the Sapindus fermentation filtrate provided in the first aspect.

[0030] In a third aspect, the present application provides a cosmetic product comprising the Sapindus fermentation filtrate prepared by the method for preparing the Sapindus fermentation filtrate provided in the first aspect, or the Sapindus fermentation filtrate provided in the second aspect. BRIEF DESCRIPTION OF DRAWINGS

[0031] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed in the embodiments will be briefly introduced as follows. It should be understood that the following drawings only show some embodiments of the present application, and therefore should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can also be obtained without creative labor on the basis of these drawings.

[0032] Figure 1 Absorbance concentration standard curve in polysaccharide detection experiment of the present application;

[0033] Figure 2 Gene expression results of TRPV1 in skin barrier efficacy detection of the present application;

[0034] Figure 3 Gene expression results of TRPV3 in skin barrier efficacy detection of the present application. DETAILED DESCRIPTION

[0035] To make the objectives, technical solutions, and advantages of the embodiments of the present application more clear, the technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Generally, the components of the embodiments of the present application described and shown in the drawings herein can be arranged and designed in various different configurations.

[0036] Therefore, the following detailed description of the embodiments of the present application provided in the accompanying drawings is not intended to limit the scope of the present application for protection, but merely represents selected embodiments of the present application. All other embodiments obtained by persons of ordinary skill in the art based on the embodiments in the present application without creative work are within the scope of protection of the present application.

[0037] The present application provides a method for preparing a soapberry fermentation filtrate, the method comprising:

[0038] Provide the residue after water extraction of soapberry;

[0039] The Schizophyllum fungus liquid is treated with a pulsed electric field, and then cultured with the residue of the soapberry extract in a culture medium, and sodium chloride is added in the middle and late stages of the culture to continue the culture.

[0040] Then add chitosan hydrochloride, stir evenly, centrifuge, filter and take the filtrate.

[0041] In the above technical solution of the present application, the residue after water extraction of soapberry is cultured with the bacterial liquid of Schizophyllum, which can realize the decomposition and dissolution of the components not dissolved in water through biological fermentation by utilizing bio-fermentation technology. The soapberry fermentation filtrate can be used to develop cosmetics with the effects of improving skin barrier, removing yellowness and brightening, thereby greatly improving the utilization rate of soapberry and facilitating the recycling of resources.

[0042] Furthermore, the inventors discovered that fermenting the residue of soapberry extract with Schizophyllum mukorossi significantly increased the yield of active substances, while other strains had only moderate effects.

[0043] The preparation method of the sapindus fermenting filtrate provided in the application greatly improves the yield of the fermentation of the schizophyllum commune by treating the schizophyllum commune liquid with a pulsed electric field and culturing the treated schizophyllum commune liquid and the residue after water extraction of the sapindus in a culture medium; adding sodium chloride in the middle and later stages of the culturing can promote the fermentation efficiency of the schizophyllum commune in the middle and later stages, improve the output of the active substances, improve the activity of the schizophyllum commune, prevent the schizophyllum commune from aging due to excessive reproduction, and effectively ensure the fermentation efficiency; simultaneously adding chitosan hydrochloride can speed up the subsequent centrifugal filtration, because the liquid is viscous, it is difficult to handle in the production process, the addition of chitosan hydrochloride can quickly separate the bacteria and the fermentation liquid, and the filtration after the addition of chitosan hydrochloride is more clear. The preparation method provided in the application has various steps that cooperate with each other, and greatly improves the content of the active substances in the residue after water extraction of the sapindus; and the obtained active substances have excellent effects of improving the skin barrier, removing yellow and brightening.

[0044] The preparation method of the sapindus fermenting filtrate provided in the application greatly improves the yield of the fermentation of the schizophyllum commune by treating the schizophyllum commune liquid with a pulsed electric field and culturing the treated schizophyllum commune liquid and the residue after water extraction of the sapindus in a culture medium; adding sodium chloride in the middle and later stages of the culturing can promote the fermentation efficiency of the schizophyllum commune in the middle and later stages, improve the output of the active substances, improve the activity of the schizophyllum commune, prevent the schizophyllum commune from aging due to excessive reproduction, and effectively ensure the fermentation efficiency; simultaneously adding chitosan hydrochloride can speed up the subsequent centrifugal filtration, because the liquid is viscous, it is difficult to handle in the production process, the addition of chitosan hydrochloride can quickly separate the bacteria and the fermentation liquid, and the filtration after the addition of chitosan hydrochloride is more clear. The preparation method provided in the application has various steps that cooperate with each other, and greatly improves the content of the active substances in the residue after water extraction of the sapindus; and the obtained active substances have excellent effects of improving the skin barrier, removing yellow and brightening.

[0045] The preparation method of the sapindus fermenting filtrate provided in the application greatly improves the yield of the fermentation of the schizophyllum commune by treating the schizophyllum commune liquid with a pulsed electric field and culturing the treated schizophyllum commune liquid and the residue after water extraction of the sapindus in a culture medium; adding sodium chloride in the middle and later stages of the culturing can promote the fermentation efficiency of the schizophyllum commune in the middle and later stages, improve the output of the active substances, improve the activity of the schizophyllum commune, prevent the schizophyllum commune from aging due to excessive reproduction, and effectively ensure the fermentation efficiency; simultaneously adding chitosan hydrochloride can speed up the subsequent centrifugal filtration, because the liquid is viscous, it is difficult to handle in the production process, the addition of chitosan hydrochloride can quickly separate the bacteria and the fermentation liquid, and the filtration after the addition of chitosan hydrochloride is more clear. The preparation method provided in the application has various steps that cooperate with each other, and greatly improves the content of the active substances in the residue after water extraction of the sapindus; and the obtained active substances have excellent effects of improving the skin barrier, removing yellow and brightening.

[0046] At present, the sapindus medicinal material is generally extracted by water extraction, and the active ingredients in the sapindus medicinal material are extracted for use. The residue after water extraction of the sapindus medicinal material is usually directly treated as waste.

[0047] However, the inventors have found through research that the residue after water extraction of the sapindus medicinal material also contains active substances, and the current conventional method has the problem of resource waste. The utilization rate of the sapindus is low.

[0048] In the above technical solution of the application, the residue after water extraction of the sapindus and the schizophyllum commune liquid are cultured, the biological fermentation technology can be used to decompose and dissolve the components that are not dissolved in water, the sapindus fermenting filtrate can be used to develop cosmetics with the effects of improving the skin barrier, removing yellow and brightening, thereby greatly improving the utilization rate of the sapindus and being beneficial to improving the resource recycling.

[0049] Further, in some embodiments of the application, the preparation method of the sapindus fermenting filtrate comprises the following steps:

[0050] Step S1, preparing the residue after water extraction of the sapindus.

[0051] In some embodiments of the application, the preparation of the residue after water extraction of the sapindus comprises:

[0052] Mixing the sapindus medicinal material with water, extracting at 60-90°C for 0.5-1.0 h, and taking the residue after centrifugation.

[0053] Further optionally, in some embodiments of the application, the preparation of the residue after water extraction of the sapindus comprises:

[0054] Mixing the Sapindus officinalis medicinal material with water, extracting at 61℃-89℃ for 0.6h-0.9h, and taking the residue after centrifugation.

[0055] Exemplarily, in some embodiments of the present application, the residue after water extraction of the Sapindus officinalis is prepared, comprising:

[0056] Mixing the Sapindus officinalis medicinal material with water, extracting at 62℃, 65℃, 68℃, 70℃, 75℃, 80℃, 85℃ for 0.6h, 0.7h, 0.8h or 0.9h, and taking the residue after centrifugation.

[0057] Further, in some embodiments of the present application, the mixing of the Sapindus officinalis medicinal material with water comprises:

[0058] Mixing the Sapindus officinalis medicinal material with water according to a mass ratio of 1:(5-10).

[0059] Further optionally, in some embodiments of the present application, the mixing of the Sapindus officinalis medicinal material with water comprises:

[0060] Mixing the Sapindus officinalis medicinal material with water according to a mass ratio of 1:(5.1-9.9).

[0061] Exemplarily, in some embodiments of the present application, the mixing of the Sapindus officinalis medicinal material with water comprises:

[0062] Mixing the Sapindus officinalis medicinal material with water according to a mass ratio of 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9 or 1:9.5.

[0063] Further optionally, in some embodiments of the present application, before the above mixing of the Sapindus officinalis medicinal material with water and extraction, the Sapindus officinalis medicinal material is crushed.

[0064] Step S2, preparing Schizophyllum commune liquid.

[0065] In some embodiments of the present application, the above Schizophyllum commune is Schizophyllum commune with the number GDMCC 5.43 from Guangdong Microbial Culture Collection Center.

[0066] Further, in some embodiments of the present application, the above Schizophyllum commune is expanded to prepare the liquid. Further optionally, in some embodiments of the present application, the above expansion of the Schizophyllum commune can be performed by a method commonly used in the art, which will not be described here.

[0067] Further, in some embodiments of the present application, the concentration of the Schizophyllum commune liquid in the liquid is 50%-80% by weight of the mycelium.

[0068] Further optionally, in some embodiments of the present application, the concentration of the Schizophyllum liquid in the Schizophyllum liquid is 51% to 79% by weight of the mycelium wet weight / liquid weight.

[0069] Illustratively, in some embodiments of the present application, the concentration of the Schizophyllum liquid in the Schizophyllum liquid is 52%, 55%, 58%, 60%, 65%, 70%, 72%, 75% or 78% by weight of the mycelium wet weight / liquid weight.

[0070] Further, in some embodiments of the present application, the Schizophyllum liquid described above is also treated by pulsed electric field.

[0071] After the Schizophyllum is treated by pulsed electric field, the activity of the Schizophyllum can be improved, more active substances can be secreted, the culture period of the Schizophyllum can be shortened, the production efficiency can be improved, the energy consumption and use time of the equipment can be reduced, the production batches can be increased, and the yield of the Schizophyllum fermentation can be improved.

[0072] Further, in some embodiments of the present application, the Schizophyllum liquid is treated by pulsed electric field, which comprises:

[0073] The pulsed electric field strength is 1KV / cm to 4KV / cm, and the cycle treatment is 20 to 60 times.

[0074] Further optionally, in some embodiments of the present application, the Schizophyllum liquid is treated by pulsed electric field, which comprises:

[0075] The pulsed electric field strength is 1.1KV / cm to 3.9KV / cm, and the cycle treatment is 21 to 59 times.

[0076] Illustratively, in some embodiments of the present application, the Schizophyllum liquid is treated by pulsed electric field, which comprises:

[0077] The pulsed electric field strength is 1.2KV / cm, 1.5KV / cm, 1.8KV / cm, 2.0KV / cm, 2.2KV / cm, 2.5KV / cm, 2.8KV / cm, 3.0KV / cm, 3.2KV / cm, 3.5KV / cm or 3.8KV / cm, and the cycle treatment is 22, 25, 28, 30, 35 or 38 times.

[0078] Step S3, culturing and fermenting in the culture medium.

[0079] Further, in some embodiments of the present application, the Schizophyllum liquid prepared in the foregoing step S2 is treated by pulsed electric field.

[0080] Furthermore, in some embodiments of the present application, the amount of the medicinal residue added is 2% to 8% of the volume of the culture medium, and the amount of the Schizophyllum bacterial liquid added is 5% to 20% of the volume of the culture medium, calculated by volume percentage.

[0081] Further optionally, in some embodiments of the present application, the residues after water extraction of soapberry and the schizophyllum liquid are cultured and fermented in a culture medium, and the amount of the residues added is 2.1% to 7.9% of the volume of the culture medium, calculated by volume percentage; illustratively, in some embodiments of the present application, the residues after water extraction of soapberry and the schizophyllum liquid are cultured and fermented in a culture medium, and the amount of the residues added is 2.5%, 2.8%, 3.0%, 3.2%, 3.5%, 3.8%, 4.0%, 4.5%, 5.0%, 5.5%, 6.0%, 6.5%, 7.0% or 7.5% of the volume of the culture medium, calculated by volume percentage.

[0082] Further optionally, in some embodiments of the present application, the residues after water extraction of soapberry and Schizophyllum schizophyllum liquid are cultured and fermented in a culture medium, and the amount of Schizophyllum schizophyllum liquid added is 5.1% to 19% of the volume of the culture medium, calculated by volume percentage. Exemplarily, the residues after water extraction of soapberry and Schizophyllum schizophyllum liquid are cultured and fermented in a culture medium, and the amount of Schizophyllum schizophyllum liquid added is 5.5%, 6%, 8%, 10%, 15% or 18% of the volume of the culture medium, calculated by volume percentage.

[0083] Furthermore, in some embodiments of the present application, the culture and fermentation comprises:

[0084] Culture in the culture medium at 27°C to 30°C for 1 to 6 days.

[0085] Further optionally, in some embodiments of the present application, the culturing and fermenting comprises:

[0086] The cells are cultured in a culture medium at 27°C, 28°C, 29°C or 30°C for 1 day, 2 days, 3 days, 4 days, 5 days or 6 days.

[0087] Furthermore, in some embodiments of the present application, the culture and fermentation comprises:

[0088] After culturing in a culture medium at 27°C to 30°C for 1 to 2 days, sodium chloride is added and the culture is continued for 2 to 4 days.

[0089] Adding sodium chloride in the middle and late stages of culture can promote the fermentation efficiency of Schizophyllum in the middle and late stages, increase the output of active substances, improve the activity of Schizophyllum, prevent Schizophyllum from aging due to excessive reproduction, and effectively ensure fermentation efficiency.

[0090] Furthermore, in some embodiments of the present application, the amount of sodium chloride added is 5% to 20% of the volume of the culture medium, calculated as a percentage by volume.

[0091] Further optionally, in some embodiments of the present application, the sodium chloride is added in an amount of 6% to 19% of the volume of the culture medium.

[0092] Illustratively, in some embodiments of the present application, the sodium chloride is added in an amount of 7%, 8%, 10%, 12%, 15% or 18% of the volume of the culture medium.

[0093] The culture medium described above can be a culture medium commonly used in the art that can be suitable for Schizophyllum culture.

[0094] Step S4, taking the filtrate.

[0095] Further, in some embodiments of the present application, the fermentation product obtained in the aforementioned step S3 is taken as a filtrate.

[0096] The fermentation filtrate described above can be used as an efficacy material for cosmetics, and can be used to develop cosmetics with the efficacy of improving skin barrier, lightening and the like.

[0097] Further, in some embodiments of the present application, taking the filtrate includes:

[0098] The chitosan hydrochloride is added to the fermentation product, mixed uniformly, centrifuged, filtered, and a filtrate is obtained.

[0099] In the technical solution described above, by adding chitosan hydrochloride, the subsequent centrifugation and filtration can be accelerated. Since the bacterial solution is viscous, it is difficult to handle during production. The addition of chitosan hydrochloride can quickly separate the Schizophyllum from the fermentation filtrate, and the subsequent filtration is more clear. Thus, it is beneficial to obtain a more pure filtrate. The application of this filtrate to cosmetics can serve as an efficacy material for cosmetics, thereby facilitating the improvement of the efficacy of the cosmetics. This filtrate can be applied to cosmetics to improve the skin barrier efficacy and lightening efficacy of the cosmetics.

[0100] Further, in some optional embodiments of the present application, the chitosan hydrochloride is added in an amount of 2.0% to 5.0% of the mass of the fermentation product.

[0101] Further optionally, in some embodiments of the present application, the chitosan hydrochloride is added in an amount of 2.1% to 4.9% of the mass of the fermentation product.

[0102] Illustratively, in some embodiments of the present application, the chitosan hydrochloride is added in an amount of 2.2%, 2.5%, 2.8%, 3.0%, 3.2%, 3.5%, 3.8%, 4.0%, 4.2%, 4.5% or 4.8% of the mass of the fermentation product.

[0103] Some embodiments of the present application provide a soapberry fermentation filtrate prepared by the method for preparing a soapberry fermentation filtrate according to any one of the preceding embodiments.

[0104] Some embodiments of the present application provide a cosmetic comprising a soapberry fermentation filtrate prepared by the method for preparing a soapberry fermentation filtrate according to any one of the preceding embodiments; or comprising a soapberry fermentation filtrate according to any one of the preceding embodiments.

[0105] Some specific examples are listed below to better illustrate the present application.

[0106] Example 1

[0107] A soapberry fermentation filtrate is provided, which is prepared according to the following steps:

[0108] (1) Take soapberry medicinal materials and crush them. Add 1:7 (soapberry medicinal materials: water) by mass ratio, stir at 80°C for 1.0 h, and take the residue after centrifugation.

[0109] (2) Schizophyllum commune liquid preparation: Schizophyllum commune (GDMCC 5.43, Guangdong Microbial Culture Collection Center) is used to expand the culture to obtain a liquid with a concentration of 70% (mycelium wet weight / liquid weight). Then the liquid is subjected to a pulsed electric field with an electric field strength of 2KV / cm and a cycle number of 40 times to obtain the Schizophyllum commune liquid.

[0110] (3) Take a certain volume of culture medium, the soapberry residue prepared in step (1), and the Schizophyllum commune liquid prepared in step (2) and culture at 28°C. After 2 days of culture, 0.6% of sodium chloride is added and the culture is continued for another 2 days. The amount of soapberry residue added is 4% of the volume of the culture medium, and the amount of Schizophyllum commune liquid added is 10% of the volume of the culture medium.

[0111] (4) Add chitosan hydrochloride to the fermentation product obtained in step (3), stir until uniform, and then stand for 2.0 h, centrifuge, filter, and obtain the filtrate. The amount of chitosan hydrochloride added is 4% of the mass of the fermentation product.

[0112] Example 2

[0113] A soapberry fermentation filtrate is provided, which is prepared according to the following steps:

[0114] (1) Take soapberry medicinal materials and crush them. Add 1:5 (soapberry medicinal materials: water) by mass ratio, stir at 70°C for 0.5 h, and take the residue after centrifugation.

[0115] (2) Schizophyllum liquid preparation: Schizophyllum (GDMCC 5.43, Guangdong Microbial Culture Collection Center) was used to obtain a liquid with a concentration of 60% (wet weight of mycelium / weight of liquid) by expansion culture. Then the liquid was subjected to a pulsed electric field with an electric field strength of 3KV / cm and a cycle number of 50 times to obtain the Schizophyllum liquid.

[0116] (3) A certain volume of culture medium, the soapberry residue prepared in step (1), and the Schizophyllum liquid prepared in step (2) were cultured at 28°C. After 2 days of culture, 1.0% of sodium chloride was added and the culture was continued for 3 days. The soapberry residue was added in an amount of 6% of the volume of the culture medium, and the Schizophyllum liquid was added in an amount of 5% of the volume of the culture medium.

[0117] (4) Chitosan hydrochloride was added to the fermentation product obtained in step (3), stirred uniformly, and then allowed to stand for 2.0h. After centrifugation and filtration, a filtrate was obtained. The amount of chitosan hydrochloride added was 4% of the mass of the fermentation product.

[0118] Example 3

[0119] A soapberry fermentation filtrate is provided, which is prepared according to the following steps:

[0120] (1) Soapberry medicinal materials were crushed. In terms of mass ratio, 1:10 (soapberry medicinal materials: water) was added, stirred at 90°C for 0.5h, and then centrifuged to obtain the residue.

[0121] (2) Schizophyllum liquid preparation: Schizophyllum (GDMCC 5.43, Guangdong Microbial Culture Collection Center) was used to obtain a liquid with a concentration of 80% (wet weight of mycelium / weight of liquid) by expansion culture. Then the liquid was subjected to a pulsed electric field with an electric field strength of 4KV / cm and a cycle number of 60 times to obtain the Schizophyllum liquid.

[0122] (3) A certain volume of culture medium, the soapberry residue prepared in step (1), and the Schizophyllum liquid prepared in step (2) were cultured at 28°C. After 1 day of culture, 0.8% of sodium chloride was added and the culture was continued for 4 days. The soapberry residue was added in an amount of 8% of the volume of the culture medium, and the Schizophyllum liquid was added in an amount of 20% of the volume of the culture medium.

[0123] (4) Chitosan hydrochloride was added to the fermentation product obtained in step (3), stirred uniformly, and then allowed to stand for 2.0h. After centrifugation and filtration, a filtrate was obtained. The amount of chitosan hydrochloride added was 5.0% of the mass of the fermentation product.

[0124] Example 4

[0125] A soapberry fermentation filtrate is provided, which is prepared according to the following steps:

[0126] (1) Take the soapberry medicinal material, crush. Add 1:7 (soapberry medicinal material: water) by mass ratio, stir at 80°C for 1.0 h, and take the residue after centrifugation.

[0127] (2) Schizophyllum liquid preparation: Schizophyllum (GDMCC 5.43, Guangdong Microbial Culture Collection Center) is used to obtain a liquid with a concentration of 70% (mycelium wet weight / liquid weight).

[0128] (3) A certain volume of culture medium, soapberry residue prepared in step (1), and Schizophyllum liquid prepared in step (2) are cultured at 28°C. After 2 days of culture, 0.6% of sodium chloride is added and the culture is continued for another 2 days. The soapberry residue is added in an amount of 4% of the volume of the culture medium, and the Schizophyllum liquid is added in an amount of 10% of the volume of the culture medium.

[0129] (4) Chitosan hydrochloride is added to the fermentation product obtained in step (3), stirred uniformly, and then left to stand for 2.0 h. After centrifugation and filtration, the filtrate is obtained. The amount of chitosan hydrochloride added is 4.0% of the mass of the fermentation product.

[0130] Example 5

[0131] A soapberry fermentation filtrate is provided, which is prepared according to the following steps:

[0132] (1) Take the soapberry medicinal material, crush. Add 1:5 (soapberry medicinal material: water) by mass ratio, stir at 70°C for 0.5 h, and take the residue after centrifugation.

[0133] (2) Schizophyllum liquid preparation: Schizophyllum (GDMCC 5.43, Guangdong Microbial Culture Collection Center) is used to obtain a liquid with a concentration of 60% (mycelium wet weight / liquid weight). Then the liquid is subjected to a pulsed electric field with an electric field strength of 3KV / cm and a cycle number of 50 times to obtain the Schizophyllum liquid.

[0134] (3) A certain volume of culture medium, soapberry residue prepared in step (1), and Schizophyllum liquid prepared in step (2) are cultured at 28°C for 5 days. The soapberry residue is added in an amount of 6% of the volume of the culture medium, and the Schizophyllum liquid is added in an amount of 5% of the volume of the culture medium.

[0135] (4) Chitosan hydrochloride is added to the fermentation product obtained in step (3), stirred uniformly, and then left to stand for 2.0 h. After centrifugation and filtration, the filtrate is obtained. The amount of chitosan hydrochloride added is 4% of the mass of the fermentation product.

[0136] Example 6

[0137] A soapberry fermentation filtrate is provided, which is prepared according to the following steps:

[0138] (1) Take the medicinal material of Sapindus, and crush it. Add 1:10 (Sapindus medicinal material: water) by mass ratio, stir at 90°C for 0.5 h, and take the residue after centrifugation.

[0139] (2) Schizophyllum liquid preparation: Schizophyllum (GDMCC 5.43, Guangdong Microbial Culture Collection Center) is used to obtain a liquid with a concentration of 80% (mycelium wet weight / liquid weight) by expansion culture. Then the liquid is put into a pulsed electric field, and an electric field strength of 4KV / cm is used for 60 cycles to obtain the Schizophyllum liquid.

[0140] (3) A certain volume of culture medium, the residue of Sapindus prepared in step (1), and the Schizophyllum liquid prepared in step (2) are cultured at 28°C. After 1 day of culture, 0.8% of sodium chloride is added, and the culture is continued for 4 days. The addition amount of the residue of Sapindus is 8% of the volume of the culture medium, and the addition amount of the Schizophyllum liquid is 20% of the volume of the culture medium.

[0141] (4) The fermentation product obtained in step (3) is allowed to stand for 2.0 h, centrifuged, filtered, and the filtrate is obtained.

[0142] Example 7

[0143] A Sapindus fermentation filtrate is provided, which is prepared according to the following steps:

[0144] (1) Take the medicinal material of Sapindus, and crush it. Add 1:10 (Sapindus medicinal material: water) by mass ratio, stir at 90°C for 0.5 h, and take the residue after centrifugation.

[0145] (2) Schizophyllum liquid preparation: Schizophyllum (GDMCC 5.43, Guangdong Microbial Culture Collection Center) is used to obtain a liquid with a concentration of 80% (mycelium wet weight / liquid weight) by expansion culture. Then the liquid is put into a pulsed electric field, and an electric field strength of 4KV / cm is used for 60 cycles to obtain the Schizophyllum liquid.

[0146] (3) A certain volume of culture medium, the residue of Sapindus prepared in step (1), and the Schizophyllum liquid prepared in step (2) are cultured at 28°C. After 1 day of culture, 0.8% of sodium chloride is added, and the culture is continued for 4 days. The addition amount of the residue of Sapindus is 8% of the volume of the culture medium, and the addition amount of the Schizophyllum liquid is 20% of the volume of the culture medium.

[0147] (4) The fermentation product obtained in step (3) is allowed to stand for 2.0 h, centrifuged, filtered, and the filtrate is obtained.

[0148] Example 8

[0149] A Sapindus fermentation filtrate is provided, which is prepared according to the following steps:

[0150] (1) Take soapberry medicinal material, crush. Add 1:10 (soapberry medicinal material: water) by mass ratio, stir at 90°C for 0.5h, after centrifugation, take the residue.

[0151] (2) Schizophyllum liquid preparation: adopt Schizophyllum (GDMCC 5.43, Guangdong Province Microbial Strain Preservation Center), expand culture to obtain a liquid with a concentration of 60% (mycelium wet weight / liquid weight).

[0152] (3) Take a certain volume of culture medium, the soapberry residue prepared in step (1), and the Schizophyllum liquid prepared in step (2) are cultured at 28°C, and cultured for 6 days. The soapberry residue is added in an amount of 5% of the volume of the culture medium; the Schizophyllum liquid is added in an amount of 10% of the volume of the culture medium.

[0153] (4) The fermentation product obtained in step (3) is allowed to stand for 2.0h, centrifuged, filtered, and the filtrate is obtained.

[0154] Experimental Example 1

[0155] The polysaccharide content in the soapberry fermentation filtrate prepared in Examples 1-8 is detected.

[0156] The phenol-sulfuric acid method is used for detection.

[0157] Anhydrous glucose reference solution is prepared by accurately weighing an appropriate amount of anhydrous glucose reference material and adding water to make a solution containing 0.1mg per 1mL.

[0158] Each sample is placed in a 10ml test tube with a stopper, and each is added with water to 1.0ml. 1mL of 5% phenol solution is accurately added quickly, and immediately after shaking, 5mL of concentrated sulfuric acid is added immediately, and placed at room temperature for 30 minutes. The corresponding reagent is used as a blank, and the absorbance is measured at a wavelength of 490nm. The standard curve is drawn with absorbance as the vertical coordinate and concentration as the horizontal coordinate. See the attached Figure 1 .

[0159] The test solution is prepared by taking Examples 1-8, adding 7 times the volume of anhydrous ethanol, placing at 4°C for 12 hours, centrifuging, discarding the supernatant, dissolving the precipitate with hot water, cooling, diluting, and shaking to obtain the test solution. The same method is used to measure the absorbance, and the content of anhydrous glucose in the test solution is read from the standard curve, and calculated.

[0160] The detection results of each example are shown in Table 1.

[0161] Table 1

[0162] Sample Content (mg / mL) Example 1 57.8 Example 2 49.6 Example 3 42.5 Example 4 30.7 Example 5 35.9 Example 6 34.1 Example 7 29.7 Example 8 18.7

[0163] From the detection results of Table 1 above, it can be seen that the polysaccharide content is detected in the sapindus fermenting filtrate of each embodiment of the present application; among them, the polysaccharide content of Example 1 reaches 57.8; it can be seen that the method of the present application can effectively utilize sapindus medicinal material residues, improve the utilization degree of residues, and save resources. The sapindus fermenting filtrate containing polysaccharides can be used for cosmetic efficacy, and is expected to develop cosmetics with the effects of improving skin barrier and removing yellow and brightening.

[0164] The polysaccharide content of Examples 1-3 of the present application reaches 42.5-57.8, with very excellent activity substance acquisition rate.

[0165] Experimental Example 2

[0166] The skin barrier effect of the sapindus fermenting filtrate prepared in Examples 1-8 was detected.

[0167] Gene expression method was used for detection.

[0168] (1) Transient receptor potential vanilloid subfamily 1 (TRPV1), also known as capsaicin receptor (VR1), is a class of ligand-gated non-selective cation channels that can be activated by capsaicin, heat (>43℃), and acid (p H<6.0). It has high permeability. Reducing the secretion and release of TRPV1 improves the skin barrier function, thereby playing an anti-itching role in dry skin, and at the same time, through up-regulating the activation of the excitatory p NR1-p Ca MKII pathway to mediate the transmission of acupuncture signals to the central nervous system, it plays an acupuncture effect, and is one of the key molecules for sensing pain. By reducing the gene expression of TRPV1, the skin barrier is improved. 2+ has high permeability. Reducing the secretion and release of TRPV1 improves the skin barrier function, thereby playing an anti-itching role in dry skin, and at the same time, through up-regulating the activation of the excitatory p NR1-p Ca MKII pathway to mediate the transmission of acupuncture signals to the central nervous system, it plays an acupuncture effect, and is one of the key molecules for sensing pain. By reducing the gene expression of TRPV1, the skin barrier is improved.

[0169] The detection results of each embodiment are shown in the specification Figure 2 P<0.05 compared with NC.

[0170] (2) Transient receptor potential vanilloid 3 (TRPV3) is a non-selective cation channel protein located on the cell membrane, widely expressed in skin, brain, dorsal root ganglion, heart and colon, etc. TRPV3 is involved in sensory transmission, skin barrier formation, hair growth and vasodilation, etc. It is proved to be closely related to pathological processes such as itching, skin inflammatory diseases and cancer. TRPV3 is involved in physiological processes such as sensory transmission, skin barrier formation and vasodilation, and knocking out TRPV3 or using TRPV3 inhibitors can relieve itching, skin inflammation and improve skin barrier.

[0171] The detection results of each embodiment are shown in the specification Figure 3 P<0.05 compared with NC.

[0172] From the above fund expression results, it can be seen that the sapindus fermentation filtrates provided by various embodiments of the application all have good skin barrier improvement effects. Examples 1-3 exhibit excellent skin barrier improvement effects.

[0173] Experimental Example 3

[0174] The yellow-removing and lightening effects of the sapindus fermentation filtrates prepared in Examples 1-8 were detected.

[0175] The non-enzyme glycosylation (NEG) reaction in vivo refers to the process in which the aldehyde group or ketone group of a reducing sugar reacts with a free amino group in a macromolecule such as a protein to form a reversible or irreversible conjugate without enzyme catalysis. The accumulation of a large amount of non-enzyme glycosylation end products is the main cause of yellowish and dull skin, and if the non-enzyme glycosylation reaction can be inhibited, the accumulation of fluorescent substances in the skin can be effectively prevented, thereby improving the skin color and lightening the skin.

[0176] Experimental procedure:

[0177] The NEG reaction of bovine serum albumin and glyoxal under certain conditions generates fluorescent products, which can simulate the NEG reaction in the human body.

[0178] In 100 mL of PBS (100 mmol·L-1phosphate buffer, pH = 7.4), 1.7 g of glyoxal and 1.0 g of BSA were sequentially added and dissolved to form a reaction solution. 1 mL of aminoguanidine (100 mmol·L-1) solution or each sample of the examples was mixed with 1 mL of the reaction solution, and incubated at 37°C for 72 h. The fluorescence detection conditions were excitation wavelength 440 nm, slit 10 nm, emission wavelength 480 nm, slit 10 nm, and detection range 480 nm.

[0179] Inhibition rate % = [1-(A1-A2) / A0] x 100%

[0180] In the formula, A1 is the fluorescence intensity of each sample group at 480 nm; A2 is the fluorescence intensity of each sample itself at 480 nm; and A0 is the fluorescence intensity of the blank control group at 480 nm.

[0181] The detection results are shown in Table 2. The higher the inhibition rate, the better the yellow-removing and lightening effect.

[0182] Table 2

[0183]

[0184]

[0185] From the experimental results of Table 2 above, it can be seen that the sapindus fermentation filtrates provided by various embodiments of the present application all have good yellow-removing and lightening effects; and the yellow-removing and lightening effects of embodiments 1-3 are as high as 91.6% to 98.2%, showing very excellent yellow-removing and lightening effects.

[0186] The above-described embodiments are merely some, but not all, of the embodiments of the present application. The detailed description of the embodiments of the present application is not intended to limit the scope of the claimed present application, but merely represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work, fall within the scope of protection of the present application.

Claims

1. A method for preparing a Ricinus communis ferment filtrate, characterized by, The method comprises: providing a water-extracted sapindus drug residue; treating the schizophyllum liquid with a pulsed electric field, and then culturing the schizophyllum liquid and the water-extracted sapindus drug residue in a culture medium; adding sodium chloride in the late culture stage and continuing the culture; then adding chitosan hydrochloride, stirring uniformly, centrifuging, filtering, and taking the filtrate; the intensity of the pulsed electric field is 1 KV / cm~4KV / cm, the number of times of the pulsed electric field cycle treatment is 20~60 times; the addition amount of the drug residue is 2%~8% of the volume of the culture medium; the addition amount of the schizophyllum liquid is 5%~20% of the volume of the culture medium; the concentration of the schizophyllum liquid in the schizophyllum liquid is 50%~80% in terms of mycelium wet weight / liquid weight; and the addition amount of the chitosan hydrochloride is 2.0%~5.0% of the mass of the fermentation product; the culture comprises: after culturing at 27℃~30℃ for 1 day~2 days in the culture medium, adding sodium chloride and continuing the culture for 2 days~4 days; and the addition amount of the sodium chloride is 5%~20% of the volume of the culture medium.

2. The sapindus fermentation filtrate preparation method according to claim 1, characterized in that, the water-extracted sapindus drug residue is prepared by: mixing sapindus medicinal materials with water, extracting at 60℃~90℃ for 0.5 h~1.0 h, centrifuging, and taking the drug residue.

3. The method for preparing soapberry fermentation filtrate according to claim 2, wherein the mixing of the sapindus medicinal materials with water comprises: mixing the sapindus medicinal materials with water at a mass ratio of 1:(5~10).

4. A Ricinus communis ferment filtrate, characterized in that, The sapindus fermentation filtrate is prepared by the sapindus fermentation filtrate preparation method according to any one of claims 1~3.

5. A cosmetic product, characterized by, The sapindus fermentation filtrate prepared by the sapindus fermentation filtrate preparation method according to any one of claims 1~3; or the sapindus fermentation filtrate according to claim 4.

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