Microfluidic chip and detector

Abstract

Embodiments of the present application provide a microfluidic chip and a detector, and relate to the technical field of microfluidics. The microfluidic chip comprises a chip main body and a sealing film. The chip main body is provided with a liquid storage chamber, a first quantitative chamber, a first reaction chamber, a plurality of second quantitative chambers and a plurality of second reaction chambers. The liquid storage chamber, the first quantitative chamber and the first reaction chamber are sequentially connected. The first quantitative chamber is provided with a plurality of second quantitative chambers on both sides, and the first quantitative chamber is connected with the plurality of second quantitative chambers. The plurality of second quantitative chambers are one-to-one corresponding and connected with the plurality of second reaction chambers. The plurality of second quantitative chambers are provided with at least one first pressure relief hole, and the first quantitative chamber is not provided with a first pressure relief hole. The sealing film is used to seal the first pressure relief hole, the liquid storage chamber, the first quantitative chamber, the first reaction chamber, the plurality of second quantitative chambers and the plurality of second reaction chambers. The microfluidic chip can reduce the overflow of the mixed liquid and improve the detection accuracy.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of microfluidic technology, in particular to a microfluidic chip and a detector. BACKGROUND

[0002] Microfluidic chip technology is a technology that integrates sample preparation, reaction, separation, detection and other basic operation units of biological, chemical and medical analysis processes on a micron-scale chip to automatically complete the whole analysis process. Microfluidic chip has the advantages of small size, high sensitivity and excellent analysis performance. The reaction chamber of the multi-channel and array design can save the consumption of experimental reagents and improve the reaction speed by using the significant laminar flow effect and diffusion effect of micron-scale fluid. It is gradually popularized in many fields such as chemical synthesis, protein enrichment, drug delivery, virus nucleic acid detection, periodontal cell culture, white blood cell behavior analysis, electrochemistry, soil environment monitoring, etc.

[0003] The commonly used microfluidic chip has a liquid storage chamber, a plurality of quantitative chambers and a plurality of reaction chambers. Each quantitative chamber is provided with a pressure relief hole. When DNA extraction and elution are performed, the DNA of the sample is first extracted to the liquid storage chamber for elution by using an extraction device. Then, the sealing film of the pressure relief hole is torn off, so that the external air enters the quantitative chamber through the pressure relief hole. Under the action of air pressure, the mixed liquid can flow into the quantitative chamber from the liquid storage chamber, and then flow into the reaction chamber.

[0004] The inventor found that when the mixed liquid flows from the liquid storage chamber into the quantitative chamber, a part of the mixed liquid is easy to overflow from the pressure relief hole, which causes the mixed liquid entering the reaction chamber to deviate, affecting the detection accuracy. SUMMARY

[0005] The purpose of the present application is to provide a microfluidic chip and a detector which can reduce the overflow of mixed liquid and improve the detection accuracy.

[0006] The embodiments of the present application can be implemented as follows:

[0007] In a first aspect, the present application provides a microfluidic chip, comprising:

[0008] a chip main body, wherein the chip main body is provided with a liquid storage chamber, a first quantitative chamber, a first reaction chamber, a plurality of second quantitative chambers and a plurality of second reaction chambers, the liquid storage chamber, the first quantitative chamber and the first reaction chamber are sequentially communicated; the first quantitative chamber is provided with the plurality of second quantitative chambers on both sides, and the first quantitative chamber and the plurality of second quantitative chambers are communicated, the plurality of second quantitative chambers and the plurality of second reaction chambers are one-to-one correspondingly arranged and communicated; the plurality of second quantitative chambers are provided with at least one first pressure relief hole, and the first quantitative chamber is not provided with the first pressure relief hole; and

[0009] a sealing film for sealing the first pressure relief hole, the liquid storage chamber, the first metering chamber, the first reaction chamber, the plurality of second metering chambers, and the plurality of second reaction chambers.

[0010] In an optional embodiment, the chip body is further provided with a pressure relief channel, the pressure relief channel is located on one side of the plurality of second metering chambers, and the pressure relief channel is in communication with the plurality of second metering chambers, the pressure relief channel is provided with a second pressure relief hole, and the sealing film is further used for sealing the second pressure relief hole.

[0011] In an optional embodiment, the chip body is further provided with a buffer chamber, and two ends of the buffer chamber are in communication with the liquid storage chamber and the first metering chamber, respectively.

[0012] In an optional embodiment, the chip body is further provided with a first cross-flow channel and a plurality of second cross-flow channels, the first metering chamber is in communication with the first reaction chamber through the first cross-flow channel, and each second metering chamber is in communication with each second reaction chamber through one second cross-flow channel.

[0013] In an optional embodiment, each second metering chamber is provided with the first pressure relief hole, and each first pressure relief hole is located at one end of the second metering chamber close to the second cross-flow channel.

[0014] In an optional embodiment, the number of the first metering chambers is two, and the two first metering chambers are in communication through a first flow channel, each first metering chamber is in communication with the plurality of second metering chambers, the liquid storage chamber is in communication with the first flow channel, and the two first metering chambers are not provided with the first pressure relief hole.

[0015] In a second aspect, the present application provides a detection instrument, comprising a heating device, a fluorescence detection device, a shell, and the microfluidic chip according to any one of the preceding embodiments, the heating device and the fluorescence detection device are arranged in the shell, and the microfluidic chip is arranged in the heating device.

[0016] In an optional embodiment, the heating device comprises:

[0017] an upper heating module, the upper heating module comprises an upper heating cover, a first resistor, and a first heating sheet, the upper heating cover is provided with a first heating groove, and the first resistor and the first heating sheet are arranged in the first heating groove; and

[0018] A lower heating module, the lower heating module comprises a lower heating cover, a second resistance and a second heating sheet, the lower heating cover is connected with the upper heating cover, the lower heating cover is provided with a second heating groove, the second resistance and the second heating sheet are arranged in the second heating groove;

[0019] Part of the microfluidic chip is arranged in the first heating groove, and another part of the microfluidic chip is arranged in the second heating groove.

[0020] In an optional embodiment, the upper heating module further comprises a first heating groove fixing plate and a second heating groove fixing plate, the first heating groove fixing plate and the second heating groove fixing plate are arranged in the first heating groove; the first resistance and the first heating sheet are located between the first heating groove fixing plate and the second heating groove fixing plate, and the first resistance is fixed to the first heating groove fixing plate, and the first heating sheet is fixed to the second heating groove fixing plate.

[0021] In an optional embodiment, the lower heating module further comprises a third heating groove fixing plate and a fourth heating groove fixing plate, the third heating groove fixing plate and the fourth heating groove fixing plate are arranged in the second heating groove; the second resistance and the second heating sheet are located between the third heating groove fixing plate and the fourth heating groove fixing plate, and the second resistance is fixed to the third heating groove fixing plate, and the second heating sheet is fixed to the fourth heating groove fixing plate.

[0022] In an optional embodiment, the lower heating module further comprises a spring sheet, a side of the third heating groove fixing plate away from the fourth heating groove fixing plate is provided with a first installation groove, the spring sheet is arranged in the first installation groove, part of the microfluidic chip is arranged in the first installation groove, and the spring sheet is used for fixing the microfluidic chip.

[0023] In an optional embodiment, the lower heating cover is provided with a first detection hole and a plurality of second detection holes, the first detection hole is arranged corresponding to the first reaction chamber, and the plurality of second detection holes are arranged one by one corresponding to the plurality of second reaction chambers.

[0024] In an optional embodiment, the fluorescence detection device comprises:

[0025] A fluorescence excitation module, the fluorescence excitation module is used for emitting fluorescence to the first reaction chamber and the plurality of second reaction chambers, and collecting signals; and

[0026] A driving module, comprising a slider, a motor and a motor support, the slider is connected with the fluorescence excitation module, the slider is slidably connected to the motor support, the motor is arranged on the motor support, and the motor is used to drive the slider to slide relative to the motor support and drive the fluorescence excitation module to emit fluorescence to the first reaction chamber and each second reaction chamber in turn.

[0027] In an optional embodiment, the driving module further comprises a positioning member and a photoelectric gate, the positioning member is arranged on the slider, and the photoelectric gate is arranged on the motor support.

[0028] The beneficial effects of the embodiments of the present application include:

[0029] The microfluidic chip comprises a chip body and a sealing film, the chip body is provided with a liquid storage chamber, a first metering chamber, a first reaction chamber, a plurality of second metering chambers and a plurality of second reaction chambers, the liquid storage chamber, the first metering chamber and the first reaction chamber are sequentially communicated; the first metering chamber is provided with a plurality of second metering chambers on both sides, and the first metering chamber and the plurality of second metering chambers are communicated, the plurality of second metering chambers and the plurality of second reaction chambers are one-to-one correspondingly arranged and communicated; the plurality of second metering chambers are provided with at least one first pressure relief hole, and the first metering chamber is not provided with a first pressure relief hole; the sealing film is used to seal the first pressure relief hole, the liquid storage chamber, the first metering chamber, the first reaction chamber, the plurality of second metering chambers and the plurality of second reaction chambers; in this way, since the first metering chamber and the plurality of second metering chambers are communicated, after the sealing film at the first pressure relief hole is torn off, external air can enter the second metering chamber, the first metering chamber and the liquid storage chamber in turn through the first pressure relief hole, and under the action of air pressure, the mixed liquid first flows into the first metering chamber and then flows into the second metering chamber; since the first metering chamber is not provided with a first pressure relief hole, the mixed liquid will not overflow when flowing into the first metering chamber. Therefore, the microfluidic chip can reduce the overflow of the mixed liquid and improve the detection accuracy.

[0030] The detector comprises the microfluidic chip, and has all the beneficial effects of the microfluidic chip. BRIEF DESCRIPTION OF DRAWINGS

[0031] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the embodiments. It should be understood that the following drawings only show some embodiments of the present application, and therefore should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can also be obtained without creative labor on the basis of these drawings.

[0032] Figure 1 The structural schematic diagram of the microfluidic chip provided by the embodiments of the present application;

[0033] Figure 2A structural schematic diagram of a detector provided for an embodiment of the present application is shown in the figure;

[0034] Figure 3 An exploded view of the detector provided for an embodiment of the present application is shown in the figure;

[0035] Figure 4 An exploded view of the heating device provided for an embodiment of the present application is shown in the figure;

[0036] Figure 5 A structural schematic diagram of a first heating groove fixing plate provided for an embodiment of the present application is shown in the figure;

[0037] Figure 6 A structural schematic diagram of a third heating groove fixing plate provided for an embodiment of the present application is shown in the figure;

[0038] Figure 7 A structural schematic diagram of a first view angle of a fluorescence detection device provided for an embodiment of the present application is shown in the figure;

[0039] Figure 8 A structural schematic diagram of a second view angle of a fluorescence detection device provided for an embodiment of the present application is shown in the figure;

[0040] Figure 9 A structural schematic diagram of a shell provided for an embodiment of the present application is shown in the figure;

[0041] Figure 10 A structural schematic diagram of a wire fixing member provided for an embodiment of the present application is shown in the figure.

[0042] Icon: 1000-detection instrument; 100-microfluidic chip; 110-chip main body; 111-liquid storage chamber; 112-first quantitative chamber; 113-second quantitative chamber; 1131-first pressure relief hole; 114-first reaction chamber; 115-second reaction chamber; 116-buffer chamber; 117-pressure relief channel; 1171-second pressure relief hole; 118-first cross flow channel; 119-second cross flow channel; 120-sealing film; 200-heating device; 210-upper heating module; 211-upper heating cover; 2111-first heating groove; 2112-upper heating cover body; 2113-upper heating cover plate; 212-first resistor; 213-first heating sheet; 214-first heating groove fixing plate; 2141-first fixing groove; 215-second heating groove fixing plate; 2151-second fixing groove; 220-lower heating module; 221-lower heating cover; 2211-second heating groove; 2212-lower heating cover body; 2213-lower heating cover plate; 2214-first detection hole; 2215-second detection hole; 222-second resistor; 223-second heating sheet; 224-third heating groove fixing plate; 2241-third fixing groove; 2242-first mounting groove; 225-fourth heating groove fixing plate; 2251-fourth fixing groove; 226-spring piece; 300-fluorescence detection device; 310-fluorescence excitation module; 311-fluorescence emission port; 320-driving module; 321-sliding block; 322-motor; 323-motor support; 324-positioning piece; 325-photoelectric door; 326-guiding shaft; 327-fan; 330-adaptor plate; 400-housing; 410-second mounting groove; 420-vent; 430-instrument switch; 440-power line interface; 450-battery back plate; 500-supporting device; 510-base; 520-bottom plate; 530-bottom plate supporting piece; 540-wire fixing piece; 600-circuit board; 700-mobile power supply. DETAILED DESCRIPTION

[0043] In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the following will be combined with the accompanying drawings to make a clear and complete description of the technical solutions in the embodiments of the present application. Obviously, the described embodiments are some but not all of the embodiments of the present application. The components of the embodiments of the present application described and shown in the accompanying drawings can be arranged and designed in various different configurations.

[0044] Therefore, the following detailed description of the embodiments of the present application provided in the accompanying drawings is not intended to limit the scope of the claimed application, but only represents selected embodiments of the present application. All other embodiments obtained by those of ordinary skill in the art based on the embodiments in the present application without creative labor are within the scope of protection of the present application.

[0045] It should be noted that similar reference numerals and letters refer to like items in the accompanying drawings, and once an item is defined in one drawing, it need not be further defined and explained in subsequent drawings.

[0046] In the description of the present application, it should be noted that if the terms "upper", "lower", "inner", "outer" and the like indicate the orientation or positional relationship shown in the drawings, or the orientation or positional relationship in which the product of the present application is usually placed, which is only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the device or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application.

[0047] In addition, if the terms "first", "second" and the like appear, they are only used to distinguish the description and cannot be understood as indicating or implying relative importance.

[0048] It should be noted that the features in the embodiments of the present application can be combined with each other without conflict.

[0049] As described in the background art, the commonly used microfluidic chip at present is provided with a liquid storage chamber, a plurality of quantitative chambers and a plurality of reaction chambers, each quantitative chamber is provided with a pressure relief hole, when DNA extraction and elution are carried out, first, the DNA of the sample is extracted to the liquid storage chamber by the extraction device for elution, then the sealing film of the pressure relief hole is torn off, so that the outside air enters the quantitative chamber through the pressure relief hole, under the action of air pressure, the mixed liquid can flow into the quantitative chamber from the liquid storage chamber, and then flow into the reaction chamber. However, this way of setting a pressure relief hole in each quantitative chamber, when the mixed liquid flows from the liquid storage chamber into the quantitative chamber, part of the mixed liquid is easy to overflow from the pressure relief hole, resulting in deviation of the mixed liquid entering the reaction chamber, affecting the detection accuracy.

[0050] Based on this, please refer to Figures 1-10 The embodiments of the present application provide a microfluidic chip 100 and a detector 1000, which can effectively improve the above-mentioned technical problems, that is, they can reduce the overflow of the mixed liquid and improve the detection accuracy. The microfluidic chip 100 and the detector 1000 will be described in detail below.

[0051] Please refer to Figure 1 , Figure 1 The structural schematic diagram of the microfluidic chip 100 provided in the present embodiment, combined with Figure 1, the microfluidic chip 100 comprises a chip body 110 and a sealing film 120, the chip body 110 is provided with a liquid storage chamber 111, a first quantitative chamber 112, a first reaction chamber 114, a plurality of second quantitative chambers 113 and a plurality of second reaction chambers 115, the liquid storage chamber 111, the first quantitative chamber 112 and the first reaction chamber 114 are sequentially communicated; a plurality of second quantitative chambers 113 are arranged on both sides of the first quantitative chamber 112, and the first quantitative chamber 112 is communicated with the plurality of second quantitative chambers 113, the plurality of second quantitative chambers 113 are one-to-one corresponding to and communicated with the plurality of second reaction chambers 115; the plurality of second quantitative chambers 113 are provided with at least one first pressure relief hole 1131, and the first quantitative chamber 112 is not provided with the first pressure relief hole 1131; the sealing film 120 is used for sealing the first pressure relief hole 1131, the liquid storage chamber 111, the first quantitative chamber 112, the first reaction chamber 114, the plurality of second quantitative chambers 113 and the plurality of second reaction chambers 115; in this way, since the first quantitative chamber 112 is communicated with the plurality of second quantitative chambers 113, after the sealing film 120 at the first pressure relief hole 1131 is torn off, external air can enter the second quantitative chamber 113, the first quantitative chamber 112 and the liquid storage chamber 111 in sequence through the first pressure relief hole 1131, under the action of air pressure, the mixed liquid first flows into the first quantitative chamber 112, and then flows into the second quantitative chamber 113; since the first quantitative chamber 112 is not provided with the first pressure relief hole 1131, the mixed liquid will not overflow when flowing into the first quantitative chamber 112. Therefore, the microfluidic chip 100 can reduce the overflow of the mixed liquid and improve the detection accuracy.

[0052] In addition, since the first reaction chamber 114 is not provided with the first pressure relief hole 1131, the number of the first pressure relief holes 1131 on the chip body 110 is reduced, the manufacturing process of the microfluidic chip 100 is reduced, the production efficiency is improved, and the production cost is reduced.

[0053] For those skilled in the art, it is easy to know that before DNA extraction and elution, the storage chamber is pre-buried with a reaction liquid, the first reaction chamber 114 and the plurality of second reaction chambers 115 are pre-buried with target primers, and then the liquid storage chamber 111, the first quantitative chamber 112, the plurality of second quantitative chambers 113, the first reaction chamber 114, the plurality of second reaction chambers 115 and the first pressure relief hole 1131 are sealed by the sealing film 120. In this embodiment, since the first reaction chamber 114 and the plurality of second reaction chambers 115 are arranged, different target primers can be pre-buried in each reaction chamber to realize multiplex reaction and rapid detection of a plurality of different target species DNA.

[0054] When the DNA extraction device is used to extract target DNA in the liquid storage chamber 111 and pre-buried liquid is eluted, in order to make the eluted DNA and the pre-buried liquid mix uniformly, the chip body 110 needs to be shaken left and right. Therefore, in order to provide a buffer space for the mixed liquid, combinedFigure 1 The chip body 110 is further provided with a buffer chamber 116, two ends of the buffer chamber 116 being respectively communicated with the liquid storage chamber 111 and the first metering chamber 112.

[0055] It should be noted that in the embodiment, the number of the first metering chambers 112 is 2, the two first metering chambers 112 are communicated through a first flow channel (not marked in the figure), each of the first metering chambers 112 is respectively communicated with a plurality of second metering chambers 113, the buffer chamber 116 is communicated with the first flow channel, and the two first metering chambers 112 are not provided with the first pressure relief hole 1131; therefore, the number of the first reaction chambers 114 is also 2. Of course, in other embodiments, the number of the first metering chambers 112 and the first reaction chambers 114 is not limited, which can be 1 or more. Similarly, in the embodiment, the number of the second metering chambers 113, the second reaction chambers 115 and the first pressure relief hole 1131 is 6, three second metering chambers 113 are respectively arranged on the two sides of the first metering chamber 112, and the second metering chambers 113 are also communicated through a second flow channel (not marked in the figure), and the first metering chamber 112 is communicated with the second metering chamber 113 through the second flow channel. Of course, in other embodiments, the number of the second metering chambers 113, the second reaction chambers 115 and the second pressure relief hole 1171 is not limited, as long as the respective metering flow and pressure relief functions can be realized.

[0056] Please continue to combine Figure 1 In order to further improve the pressure relief effect and make the mixed liquid flow into the first metering chamber 112 and the second metering chamber 113 better, the chip body 110 is further provided with a pressure relief channel 117, the pressure relief channel 117 being located on one side of the plurality of second metering chambers 113, and the pressure relief channel 117 being communicated with the plurality of second metering chambers 113, the pressure relief channel 117 being provided with a second pressure relief hole 1171, and the sealing film 120 being further used for sealing the second pressure relief hole 1171. That is, the pressure relief channel 117 is communicated with the second metering chambers 113 located at both ends, and the second pressure relief hole 1171 is located at one end of the pressure relief channel 117.

[0057] Since the microfluidic chip 100 needs to be inserted into the detector 1000, and the mixed liquid in the first reaction chamber 114 and the second reaction chamber 115 needs to be heated for detection, that is, the mixed liquid in the reaction chamber and the target primer perform LAMP (Loop-mediated isothermal amplification) reaction heating, in order to reduce the upward evaporation of the liquid in the heating process, the chip body 110 is further provided with a first cross flow channel 118 and a plurality of second cross flow channels 119. The first quantitative chamber 112 is in communication with the first reaction chamber 114 through the first cross flow channel 118, and each second quantitative chamber 113 is in communication with each second reaction chamber 115 through a second cross flow channel 119. The first cross flow channel 118 and the second cross flow channel 119 have such a cross channel structure, which can block the upward evaporation of the liquid in the LAMP reaction heating process to a certain extent.

[0058] It should be noted that since the width of the first cross flow channel 118 and the second cross flow channel 119 is small and less than the width of the first quantitative chamber 112 and the second quantitative chamber 113, and the first quantitative chamber 112 and the second quantitative chamber 113 are arc-shaped in structure, the mixed liquid has tension at this position. Therefore, when the mixed liquid flows into the first quantitative chamber 112 and the second quantitative chamber 113, it will not further flow into the first cross flow channel 118 and the second cross flow channel 119.

[0059] Further, in the present embodiment, each second quantitative chamber 113 is provided with a first pressure relief hole 1131, and each first pressure relief hole 1131 is located at one end of the second quantitative chamber 113 close to the second cross flow channel 119. Such a setting can make the mixed liquid more conveniently flow into the bottom of the second quantitative chamber 113 and flow to the adjacent second quantitative chamber 113 by the action of air pressure, thereby better realizing the equal division of the mixed liquid.

[0060] Please refer to Figures 2-10 , which will be described in detail below.

[0061] For those skilled in the art, it is easy to know that the detector 1000 is used for LAMP amplification of the microfluidic chip 100, and real-time detection and result interpretation.

[0062] Please refer to Figure 2 and Figure 3 , Figure 2 the structural schematic diagram of the detector 1000 provided in the present embodiment, Figure 3 the exploded view of the detector 1000 provided in the present embodiment, combined with Figure 2 and Figure 3The detector 1000 comprises a microfluidic chip 100, a heating device 200, a fluorescence detection device 300, and a shell 400. The heating device 200 and the fluorescence detection device 300 are arranged in the shell 400, and the microfluidic chip 100 is arranged on the heating device 200. The heating device 200 is used for heating the microfluidic chip 100, and the fluorescence detection device 300 is used for emitting fluorescence to the first reaction chamber 114 and the second reaction chamber 115 and collecting signals.

[0063] Further, the detector 1000 further comprises a supporting device 500, a circuit board 600, and a mobile power supply 700. The supporting device 500 is connected with the shell 400, the circuit board 600 is arranged in the shell 400, and the mobile power supply 700 is arranged on the shell 400. The circuit board 600 is electrically connected with the heating device 200, the fluorescence detection device 300, and the mobile power supply 700, and the mobile power supply 700 is further electrically connected with the heating device 200 and the fluorescence detection device 300. The detector 1000 has a small volume and is convenient to use, and can be used for on-site sampling detection in fields, large-scale agricultural markets, and the like.

[0064] Please refer to Figure 4 , Figure 4 The exploded view of the heating device 200 provided in the embodiment is shown in FIG. 2, and the heating device 200 comprises an upper heating module 210 and a lower heating module 220. Figure 4 The upper heating module 210 comprises an upper heating cover 211, a first resistor 212, and a first heating sheet 213. The upper heating cover 211 is provided with a first heating groove 2111, and the first resistor 212 and the first heating sheet 213 are arranged in the first heating groove 2111. The lower heating module 220 comprises a lower heating cover 221, a second resistor 222, and a second heating sheet 223. The lower heating cover 221 is connected with the upper heating cover 211, and the lower heating cover 221 is provided with a second heating groove 2211. The second resistor 222 and the second heating sheet 223 are arranged in the second heating groove 2211. A part of the microfluidic chip 100 is arranged in the first heating groove 2111, and another part of the microfluidic chip 100 is arranged in the second heating groove 2211.

[0065] Please refer to Figure 2 and Figure 4In the embodiment, one end of the upper heating cover 211 is rotatably connected to one end of the lower heating cover 221, so that the second heating groove 2211 can be conveniently opened, the microfluidic chip 100 is inserted into the second heating groove 2211, and then the upper heating cover 211 is buckled with the lower heating cover 221, so that the first heating groove 2111 can also fix the upper part of the microfluidic chip 100, thereby simultaneously heating the microfluidic chip 100 through the first resistance 212, the first heating sheet 213, the second resistance 222 and the second heating sheet 223. Of course, in other embodiments, the upper heating cover 211 and the lower heating cover 221 can also be detachably connected by a buckle, which can also facilitate the installation of the microfluidic chip 100.

[0066] It should be noted that in the embodiment, the first heating sheet 213 and the second heating sheet 223 are both ceramic heating sheet materials made of alumina. The first resistance 212 and the second resistance 222 are both platinum resistors, and the resistance of the platinum resistor increases significantly with the increase of temperature, so that a temperature measuring circuit can be built to convert the change of the resistance into the change of the voltage signal, thereby measuring the temperature; the resistance of the platinum resistor will increase by 0.385% when the temperature increases by 1℃, and the resistance will increase by 3.85Ω. For example, in the embodiment, the set value is set to be the resistance of the platinum resistor, which is 1000Ω, when the measured data is less than 1000Ω, the ceramic heating sheet heats according to a certain power (the lower the data, the greater the power), when the measured data reaches 1000Ω or is greater than 1000Ω, the heating is turned off until the measured data stabilizes at 1000Ω, at this time the temperature also stabilizes at 65.0℃, then the set value is directly set to 1000Ω to reach 65℃.

[0067] Please continue to combine Figure 4 Further, the upper heating module 210 further comprises a first heating groove fixing plate 214 and a second heating groove fixing plate 215, both of which are arranged in the first heating groove 2111; the first resistance 212 and the first heating sheet 213 are both located between the first heating groove fixing plate 214 and the second heating groove fixing plate 215, and the first resistance 212 is fixed to the first heating groove fixing plate 214 and the first heating sheet 213 is fixed to the second heating groove fixing plate 215. Similarly, the lower heating module 220 further comprises a third heating groove fixing plate 224 and a fourth heating groove fixing plate 225, both of which are arranged in the second heating groove 2211; the second resistance 222 and the second heating sheet 223 are both located between the third heating groove fixing plate 224 and the fourth heating groove fixing plate 225, and the second resistance 222 is fixed to the third heating groove fixing plate 224 and the second heating sheet 223 is fixed to the fourth heating groove fixing plate 225.

[0068] Specifically, the upper heating cover 211 comprises an upper heating cover body 2112 and an upper heating cover plate 2113, the first heating groove 2111 is arranged on the upper heating cover body 2112, the lower heating cover 221 comprises a lower heating cover body 2212 and a lower heating cover plate 2213, and the second heating groove 2211 is arranged on the lower heating cover body 2212. In order to facilitate the installation of the first heating groove fixing plate 214 and the second heating groove fixing plate 215, the upper heating cover plate 2113 body and the upper heating cover plate 2113 are detachably connected, and in order to facilitate the installation of the third heating groove fixing plate 224 and the fourth heating groove fixing plate 225, the lower heating cover plate 2213 body and the lower heating cover plate 2213 are detachably connected.

[0069] Please refer to Figure 5 and Figure 6 , Figure 5 the structure diagram of the first heating groove fixing plate 214 provided in the embodiment, Figure 6 the structure diagram of the third heating groove fixing plate 224 provided in the embodiment, in combination with Figures 4-6 In order to better fix the first resistor 212, the first heating sheet 213, the second resistor 222 and the second heating sheet 223, the first heating groove fixing plate 214 is provided with a first fixing groove 2141, and the first resistor 212 is arranged in the first fixing groove 2141; the second heating groove fixing plate 215 is provided with a second fixing groove 2151, and the first heating sheet 213 is arranged in the second fixing groove 2151; the third heating groove fixing plate 224 is provided with a third fixing groove 2241, and the second resistor 222 is arranged in the third fixing groove 2241; and the fourth heating groove fixing plate 225 is provided with a fourth fixing groove 2251, and the second heating sheet 223 is arranged in the fourth fixing groove 2251.

[0070] In addition, in the embodiment, the materials of the first heating groove fixing plate 214, the second heating groove fixing plate 215, the third heating groove fixing plate 224 and the fourth heating groove fixing plate 225 are all aluminum alloy, which can make the temperature of the first heating sheet 213 and the second heating sheet 223 be more uniformly transmitted to the heating area of the microfluidic chip 100, that is, the first reaction chamber 114 and the plurality of second reaction chambers 115 can be uniformly heated. The materials of the upper heating cover body 2112, the upper heating cover plate 2113, the lower heating cover body 2212 and the lower heating cover plate 2213 are polyether ether ketone, which is a special engineering plastic with excellent performance and has good heat preservation performance.

[0071] Please refer to 4 and Figure 6In order to better fix the microfluidic chip 100, the lower heating module 220 further comprises an elastic sheet 226, the third heating groove fixing plate 224 is provided with a first mounting groove 2242 on the side away from the fourth heating groove fixing plate 225, the elastic sheet 226 is arranged in the first mounting groove 2242, part of the microfluidic chip 100 is arranged in the first mounting groove 2242, and the elastic sheet 226 is used for fixing the microfluidic chip 100. It should be noted that in the embodiment, the number of the elastic sheet 226 is 2, of course, in other embodiments, the number of the elastic sheet 226 is not limited, as long as the fixing function of the microfluidic chip 100 can be realized.

[0072] Further, the lower heating cover plate 2213 is provided with a first detection hole 2214 and a plurality of second detection holes 2215, the first detection hole 2214 is arranged corresponding to the first reaction chamber 114, and the plurality of second detection holes 2215 are arranged corresponding to the plurality of second reaction chambers 115. In this way, the fluorescence detection device 300 can emit fluorescence to the first reaction chamber 114 and the plurality of second reaction chambers 115 through the first detection hole 2214 and the plurality of second detection holes 2215.

[0073] Please refer to Figure 7 and Figure 8 , Figure 7 The structure schematic diagram of the first perspective of the fluorescence detection device 300 provided in the embodiment is shown in Figure 8 The structure schematic diagram of the second perspective of the fluorescence detection device 300 provided in the embodiment is shown in Figure 7 and Figure 8 The fluorescence detection device 300 comprises a fluorescence excitation module 310 and a driving module 320, the fluorescence excitation module 310 is electrically connected with the circuit board 600, the fluorescence excitation module 310 is used for emitting fluorescence to the first reaction chamber 114 and the plurality of second reaction chambers 115, and collecting signals and transmitting the signals to the circuit board 600; the driving module 320 comprises a sliding block 321, a motor 322 and a motor support 323, the sliding block 321 is connected with the fluorescence excitation module 310, the sliding block 321 is slidably connected with the motor support 323, the motor 322 is arranged on the motor support 323, the motor 322 is electrically connected with the circuit board 600 and the mobile power supply 700 respectively, and the motor 322 is used for driving the sliding block 321 to slide relative to the motor support 323, and driving the fluorescence excitation module 310 to emit fluorescence to the first reaction chamber 114 and each second reaction chamber 115 in turn.

[0074] In detail, the fluorescence excitation module 310 is provided with a fluorescence emission port 311, it is easy to understand that the fluorescence emission port 311 emits fluorescence to the first reaction chamber 114 and the second reaction chamber 115 of the microfluidic chip 100 through the first detection hole 2214 and the second detection hole 2215, so as to complete the fluorescence excitation and signal collection process.

[0075] Further, the driving module 320 further comprises a positioning member 324 and a photoelectric door 325, the positioning member 324 is arranged on the sliding block 321, the photoelectric door 325 is arranged on the motor support 323, and the photoelectric door 325 is electrically connected with the circuit board 600. When the motor 322 drives the sliding block 321 to slide and drives the fluorescence excitation module 310 to perform the fluorescence excitation process of different reaction chambers, the sliding block 321 will drive the positioning member 324 to slide, and the photoelectric door 325 will cause a signal change of the photoelectric door 325. Specifically, when the positioning member 324 scans the photoelectric door 325, the electrical signal will change, at this time, the position of the motor 322 can be positioned; then the motor 322 is controlled to drive the sliding block 321 to move to different reaction chamber positions, and fluorescence scanning is performed, one end of the photoelectric door 325 sends light, and the other end receives light, the receiving end can convert the presence or absence of the light signal into the on-off of the circuit, so that it becomes an electrical signal; when the positioning member 324 blocks the photoelectric door 325, the photoelectric door 325 cannot receive the light signal, the circuit is disconnected, and the electrical signal disappears. That is, the photoelectric door 325 can receive light when the positioning member 324 does not block the photoelectric door 325, so that the circuit is closed and an electrical signal is generated, the electrical signal is connected with the single-chip microcomputer (not shown in the figure) in the shell 400, so that the single-chip microcomputer knows whether the photoelectric door 325 is blocked, and further judges whether the position of the motor 322 is zero. Therefore, according to the change of the signal, it can be determined in real time that the fluorescence emission port 311 is facing which detection hole and is exciting the fluorescence signal of which reaction chamber, and at the same time, the error that may be generated after the motor 322 is positioned multiple times can be eliminated.

[0076] Please continue to combine Figure 7 and Figure 8 The driving module 320 further comprises a guide shaft 326 and a fan 327, both of which are arranged on the motor support 323, and the sliding block 321 is slidably connected to the guide shaft 326. Of course, in other embodiments, a sliding rail can also be arranged on the motor support 323, and the sliding block 321 is slidably connected to the sliding rail to achieve sliding connection and drive the fluorescence excitation module 310 to move. It can be understood that the fan 327 is used for heat dissipation of the motor 322 to improve the working stability.

[0077] In order to better fix the movement of the fluorescence excitation module 310, further, the fluorescence detection device 300 further comprises an adapter plate 330, which is fixedly connected with the fluorescence excitation module 310 and the sliding block 321 respectively.

[0078] Please refer to Figure 9 , Figure 9 The structure schematic diagram of the shell 400 provided in the embodiment, combined with Figure 9The shell 400 is provided with a second mounting groove 410, a ventilation port 420 and a power line interface 440. The second mounting groove 410 is used for inserting the microfluidic chip 100, and the heating device 200 is arranged in the second mounting groove 410. The ventilation port 420 can dissipate heat from the inside of the detector 1000, so that the components inside the detector 1000 work stably. Further, the shell 400 is also provided with an instrument switch 430 and a battery backboard 450. As can be easily understood, the instrument switch 430 is electrically connected to the components inside the detector 1000, and the battery backboard 450 is used to fix the mobile power supply 700, which can improve the portability of the detector 1000.

[0079] Please continue to combine Figure 3 The support device 500 includes a base 510, a bottom plate 520, a bottom plate support 530 and a wire fixing piece 540. The base 510 is connected to the shell 400. The bottom plate 520 is located between the base 510 and the bottom plate support 530, and the bottom plate 520 is connected to the base 510 and the bottom plate support 530, respectively. The wire fixing piece 540 is arranged on the base 510. Please refer to Figure 10 , Figure 10 The structure diagram of the wire fixing piece 540 provided in the embodiment is shown in the figure. Please refer to Figure 3 and Figure 10 As can be easily understood, the wire fixing piece 540 is used to regulate and fix the wires connected to the circuit board 600.

[0080] Of course, the detector 1000 also includes some wiring terminals such as communication interfaces, USB interfaces and other communication interfaces, as well as modular chips such as Bluetooth chips and USB-to-serial chip, and a mobile phone APP software is arranged, which can transmit the data detected by the fluorescence detection device 300 to the APP through Bluetooth, so as to conveniently and real-timely obtain the fluorescence LAMP reaction amplification curve.

[0081] In summary, the working principle of the microfluidic chip 100 and the detector 1000 is as follows:

[0082] First, the operation before DNA extraction: bury the reaction liquid in the storage chamber in advance, and bury the target primer in the first reaction chamber 114 and the plurality of second reaction chambers 115. In order to perform a control test, some reaction chambers can be selected without burying the target primer. Then, the sealing film 120 is used to seal the storage chamber 111, the first quantitative chamber 112, the plurality of second quantitative chambers 113, the first reaction chamber 114, the plurality of second reaction chambers 115 and the first pressure relief hole 1131. At this time, the microfluidic chip 100 can be directly used for DNA extraction and elution. If it needs to be transported to other places, it can also be frozen for storage, which is convenient for transportation. After arriving at the destination, it can be taken out and thawed for use.

[0083] Next, the extraction and elution operation of DNA: lay the microfluidic chip 100 flat, use the DNA extraction device to extract the material on the surface of the sample to be tested, quickly transfer it to the microfluidic chip 100, and pierce the sealing film 120 to allow the sample to enter the storage chamber and mix with the reaction solution for elution for 1-3 seconds. Then shake the microfluidic chip 100 left and right to mix the eluted DNA mixture evenly. Then stand the microfluidic chip 100 upright, tear off the sealing film 120 at the first pressure relief hole 1131 and the second pressure relief hole 1171, allowing external air to enter the second quantitative chamber 113, the first quantitative chamber 112, the buffer chamber 116, and the storage chamber in turn. Under the action of air pressure, the mixture flows into the first quantitative chamber 112 through the buffer chamber 116 and then flows into each second quantitative chamber 113 in turn along the second flow channel. Then replace the pierced sealing film 120 above the storage chamber with a new sealing film 120 to seal the storage chamber, the first pressure relief hole 1131, and the second pressure relief hole 1171. Then shake the microfluidic chip 100 vigorously to allow the mixture to flow into the first reaction chamber 114 and the second reaction chamber 115 from the first cross-flow channel 118 and the second cross-flow channel 119.

[0084] Finally, the amplification operation of DNA: open the upper heating cover 211 of the heating device 200, vertically insert the end of the microfluidic chip 100 where the reaction chambers are located into the second heating groove 2211 of the lower heating cover 221, and buckle the upper heating cover 211. Turn on the switch, set the heating and fluorescence detection time, and the heating device 200 heats the first reaction chamber 114 and the second reaction chamber 115 in the microfluidic chip 100. The fluorescence detection device 300 emits fluorescence to the first reaction chamber 114 and the second reaction chamber 115 through the first detection hole 2214 and the second detection hole 2215, collects the signal, transmits the data to the APP software through Bluetooth, and observes the curve amplification result in real time. After the detection is completed, remove the microfluidic chip 100, observe the color of the mixture in the first reaction chamber 114 and the second reaction chamber 115, and compare it with the standard color card. According to the corresponding color information, determine whether it is negative or positive. Negative indicates that it does not contain the species corresponding to the primer; positive indicates that it contains the species corresponding to the primer.

[0085] Therefore, the microfluidic chip 100 can reduce the overflow of the mixture, improve the detection accuracy, and reduce the number of first pressure relief holes 1131, which can reduce the manufacturing process of the microfluidic chip 100, improve efficiency, and reduce costs. The detector 1000 includes the microfluidic chip 100, which has all the functions of the microfluidic chip 100, and the detector 1000 is small in size, convenient to use, and suitable for on-site sampling detection in fields, large-scale farmers' markets, and other situations.

[0086] The above merely illustrates the specific embodiments of the present application, but the protection scope of the present application is not limited thereto, any person skilled in the art can easily think of the changes or replacements within the technical range disclosed by the present application, which should be covered in the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A microfluidic chip, characterized by, It comprises: a chip body (110), which is provided with a liquid storage chamber (111), a first metering chamber (112), a first reaction chamber (114), a plurality of second metering chambers (113) and a plurality of second reaction chambers (115), the liquid storage chamber (111), the first metering chamber (112) and the first reaction chamber (114) are sequentially communicated; the first metering chamber (112) is provided with the plurality of second metering chambers (113) on both sides, and the first metering chamber (112) and the plurality of second metering chambers (113) are communicated, the plurality of second metering chambers (113) and the plurality of second reaction chambers (115) are one-to-one corresponding and communicated; each second metering chamber (113) is provided with a first pressure relief hole (1131), and the first metering chamber (112) is not provided with the first pressure relief hole (1131); and a sealing film (120) for sealing the first pressure relief hole (1131), the liquid storage chamber (111), the first metering chamber (112), the first reaction chamber (114), the plurality of second metering chambers (113) and the plurality of second reaction chambers (115).

2. The microfluidic chip of claim 1, wherein, The chip body (110) is further provided with a first cross-flow channel (118) and a plurality of second cross-flow channels (119), the first metering chamber (112) is communicated with the first reaction chamber (114) through the first cross-flow channel (118), and each second metering chamber (113) is communicated with each second reaction chamber (115) through one second cross-flow channel (119).

3. The microfluidic chip of claim 2, wherein, Each first pressure relief hole (1131) is located at one end of the second metering chamber (113) close to the second cross-flow channel (119).

4. The microfluidic chip of claim 1, wherein, The number of the first metering chambers (112) is two, and the two first metering chambers (112) are communicated through a first flow channel, each first metering chamber (112) is communicated with the plurality of second metering chambers (113), the liquid storage chamber (111) is communicated with the first flow channel, and the two first metering chambers (112) are not provided with the first pressure relief hole (1131).

5. The microfluidic chip of claim 1, wherein, The chip body (110) is further provided with a pressure relief channel (117), the pressure relief channel (117) is located on one side of the plurality of second metering chambers (113), and the pressure relief channel (117) is communicated with the plurality of second metering chambers (113), the pressure relief channel (117) is provided with a second pressure relief hole (1171), and the sealing film (120) is further used for sealing the second pressure relief hole (1171).

6. A detector characterized by, It comprises a heating device (200), a fluorescence detection device (300), a shell (400) and the microfluidic chip (100) of any one of claims 1-5, the heating device (200) and the fluorescence detection device (300) are arranged in the shell (400), and the microfluidic chip (100) is arranged in the heating device (200).

7. The detector of claim 6, wherein The heating device (200) comprises: An upper heating module (210) comprising an upper heating cover (211) having a first heating groove (2111) formed therein, a first resistance (212), and a first heating sheet (213), wherein the first resistance (212) and the first heating sheet (213) are arranged in the first heating groove (2111); and A lower heating module (220) comprising a lower heating cover (221) connected to the upper heating cover (211), a second resistance (222), and a second heating sheet (223), wherein the lower heating cover (221) has a second heating groove (2211) formed therein, and the second resistance (222) and the second heating sheet (223) are arranged in the second heating groove (2211). Part of the microfluidic chip (100) is arranged in the first heating groove (2111), and another part of the microfluidic chip (100) is arranged in the second heating groove (2211).

8. The detector of claim 7, wherein, The upper heating module (210) further comprises a first heating groove fixing plate (214) and a second heating groove fixing plate (215), wherein the first heating groove fixing plate (214) and the second heating groove fixing plate (215) are arranged in the first heating groove (2111); the first resistance (212) and the first heating sheet (213) are located between the first heating groove fixing plate (214) and the second heating groove fixing plate (215), and the first resistance (212) is fixed to the first heating groove fixing plate (214), and the first heating sheet (213) is fixed to the second heating groove fixing plate (215).

9. The detector of claim 7, wherein, The lower heating module (220) further comprises a third heating groove fixing plate (224) and a fourth heating groove fixing plate (225), wherein the third heating groove fixing plate (224) and the fourth heating groove fixing plate (225) are arranged in the second heating groove (2211); the second resistance (222) and the second heating sheet (223) are located between the third heating groove fixing plate (224) and the fourth heating groove fixing plate (225), and the second resistance (222) is fixed to the third heating groove fixing plate (224), and the second heating sheet (223) is fixed to the fourth heating groove fixing plate (225).

10. The detector of claim 6, wherein The fluorescence detection device (300) comprises: A fluorescence excitation module (310) configured to emit fluorescence to the first reaction chamber (114) and the plurality of second reaction chambers (115) and collect signals; and A fluorescence detection module (320) configured to detect fluorescence emitted by the first reaction chamber (114) and the plurality of second reaction chambers (115). A driving module (320) comprising a sliding block (321), a motor (322) and a motor support (323), the sliding block (321) is connected with the fluorescence excitation module (310), the sliding block (321) is slidably connected to the motor support (323), the motor (322) is arranged on the motor support (323), the motor (322) is used for driving the sliding block (321) to slide relative to the motor support (323), and the fluorescence excitation module (310) is driven to emit fluorescence to the first reaction chamber (114) and each second reaction chamber (115) in turn.

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