A multi-purpose bioreactor

Abstract

The application provides a multipurpose bioreactor, which comprises a reactor body, the reactor body comprises a tank body and a top cover detachably connected with the tank body, the tank body is provided with a plurality of interface holes penetrating the wall of the tank body, and the outer wall of the tank body is provided with an interface flange at the interface holes; the interface holes at least comprise a feeding interface hole, an air inlet interface hole, an electrode interface hole and a stirring interface hole; a disposable reaction bag can be arranged in the tank body and attached to the inner wall, the disposable reaction bag has a plurality of connecting interfaces; the connecting interfaces at least comprise a feeding interface, an air inlet interface, an electrode interface and a stirring interface; the feeding interface hole, the air inlet interface hole, the electrode interface hole and the stirring interface hole are arranged one by one corresponding to the feeding interface, the air inlet interface, the electrode interface and the stirring interface; and the top cover is detachably provided with a fixing clamp for fixing the disposable reaction bag. The application meets the requirements of various culture processes and reduces the cost.

Description

TECHNICAL FIELD

[0001] The present application relates to cell culture bioreactor equipment, in particular to a multipurpose bioreactor. BACKGROUND

[0002] Bioreactor is a device for vaccine production to provide host cell culture, accelerate cell propagation, metabolism and express the final product, the performance of the reactor directly affects the quality of the final product, and further determines the yield of vaccine.

[0003] At present, there are two types of cell culture bioreactors: one is a traditional stainless steel fixed bioreactor, which includes a reactor body, a temperature control system, an air inlet system, an exhaust system, an electrode system, an electrical automatic control system, etc.; the other is a disposable bioreactor, which is composed of a disposable bioreaction bag, a disposable air inlet system, a disposable exhaust system, a disposable electrode system, a stainless steel shell support system with temperature control capability, and an electrical automatic control system.

[0004] During cell culture, different reactor culture systems need to be selected according to different culture processes. Some need to choose traditional stainless steel fixed bioreactor, and some need to choose disposable reactor bag reactor as production culture equipment. In order to meet the long-term development and adapt to the production process demand, vaccine production enterprises need to purchase two different devices for use, which not only increases the cost of equipment procurement, but also increases the cost of plant construction and personnel use.

[0005] Therefore, it is necessary to provide a multipurpose bioreactor, which can meet the needs of various culture processes with one device to reduce the cost. SUMMARY

[0006] The present application provides a multipurpose bioreactor, which can meet the needs of various culture processes and reduce the cost.

[0007] To achieve the above purpose, the present application provides the following technical scheme.

[0008] A multi-purpose bioreactor comprises a reactor body, the reactor body comprises a tank body and a top cover detachably connected with the tank body, the tank body is provided with a plurality of interface holes penetrating the wall of the tank body, the outer wall of the tank body is provided with an interface flange at the interface hole; the interface hole comprises at least a feeding interface hole, an air inlet interface hole, an electrode interface hole and a stirring interface hole; a disposable reaction bag can be fitted in the tank body, the disposable reaction bag has a plurality of connection interfaces; the connection interface comprises at least a feeding interface, an air inlet interface, an electrode interface and a stirring interface; the feeding interface hole, the air inlet interface hole, the electrode interface hole and the stirring interface hole are arranged one by one with the feeding interface, the air inlet interface, the electrode interface and the stirring interface; the top cover is detachably provided with a fixing clamp for fixing the disposable reaction bag.

[0009] Optionally, it further comprises a feeding system, an air inlet system and a detection electrode, the feeding system is connected with the interface flange at the feeding interface hole, and communicates with the feeding interface hole or the feeding interface through the feeding interface hole; the air inlet system is connected with the interface flange at the air inlet interface hole, and communicates with the air inlet interface hole or the air inlet interface through the air inlet interface hole; the detection electrode penetrates the electrode interface hole or the electrode interface through the electrode interface hole, and is fixed through the interface flange at the electrode interface hole.

[0010] Optionally, the detection electrode comprises at least a DO detection electrode, a PH value detection electrode and a temperature detection electrode.

[0011] Optionally, it further comprises a stirring device, the stirring device comprises a reactor stirring device and a disposable reaction bag stirring device, when the tank body is not provided with a disposable reaction bag, the reactor stirring device is used, the reactor stirring device penetrates the stirring interface hole, and is fixed through the interface flange at the stirring interface hole; when the tank body is provided with a disposable reaction bag, the disposable reaction bag stirring device is used, the disposable reaction bag stirring device penetrates the stirring interface through the stirring interface hole, and is fixed through the interface flange at the stirring interface hole.

[0012] Optionally, the stirring interface hole is arranged at the bottom of the tank body, the reactor stirring device comprises a first stirring motor, a first stirring shaft and first stirring paddles, the first stirring motor is arranged outside the tank body, the first stirring shaft extends into the tank body through the stirring interface hole, and the first stirring paddles are two first stirring paddles arranged at intervals along the axial direction of the first stirring shaft; the disposable reaction bag stirring device comprises a second stirring motor, a second stirring shaft and a second stirring paddle, the second stirring motor is arranged outside the tank body, the second stirring shaft extends into the disposable reaction bag through the stirring interface hole and the stirring interface, and the second stirring paddle is one second stirring paddle arranged on the second stirring shaft.

[0013] Optionally, the temperature control structure comprises a temperature control jacket and a heat preservation layer arranged outside the temperature control jacket, the temperature control jacket is arranged on the outer wall of the tank body, and the temperature control structure is provided with a avoiding groove at the interface flange.

[0014] Optionally, the temperature control system is electrically connected with the temperature control jacket through the temperature control interface.

[0015] Optionally, the top cover lifting device is connected with the top cover and can drive the top cover to move, the top cover can be closed on the top of the tank body to seal the tank body, the top cover lifting device comprises a lifting lead screw, a lifting motor, a lifting sliding block and a lifting support, the lifting sliding block is arranged on the lifting lead screw, the lifting support is fixed to the lifting sliding block and located above the top cover, and the lifting motor drives the lifting lead screw to rotate and drives the lifting sliding block and the lifting support to move in the vertical direction.

[0016] Optionally, the exhaust system is connected with the top cover.

[0017] Optionally, the tank body support structure comprises a tank body support, the tank body support is provided with height-adjustable supporting legs, and the temperature control structure is arranged on the tank body support.

[0018] Optionally, the materials of the tank body and the top cover are stainless steel.

[0019] Compared with the prior art, the technical scheme of the embodiment of the present application has the beneficial effects.

[0020] For example, the application rationally designs the reactor body structure, sets the interface hole matched with the connection interface of the disposable reaction bag on the tank body, realizes the connection with the feeding system, the air inlet system, the detection electrode, the stirring device and the like through the interface hole, meets the use requirements of the disposable reaction bag and the fixed reactor at the same time, integrates the functions of the two types of reactors, meets the requirements of various culture processes, and reduces the equipment cost.

[0021] For another example, the application sets the detachable top cover, cooperates the top cover lifting device, realizes the automatic lifting of the top cover, sets the detachable disposable reaction bag fixing clamp on the top cover, realizes the bearing of the disposable reaction bag by the tank body, realizes the fixation of the top of the disposable reaction bag through the fixing clamp, and is more convenient to use.

[0022] For another example, the application sets two groups of reactor stirring devices and disposable reaction bag stirring devices matched with different use requirements, can adjust the stirring device according to the use requirement, and improves the production efficiency. BRIEF DESCRIPTION OF DRAWINGS

[0023] Fig. 1 is the structure schematic view of the multipurpose bioreactor in the embodiment of the application;

[0024] Fig. 2 is the structure schematic view of the multipurpose bioreactor cooperating with the disposable reaction bag in the embodiment of the application;

[0025] Fig. 3 is the use schematic view of the multipurpose bioreactor in the embodiment of the application.

[0026] BRIEF DESCRIPTION OF DRAWINGS

[0027] 1, reactor body; 11, tank body; 111, feeding interface hole; 112, air inlet interface hole; 113, electrode interface hole; 114, stirring interface hole;

[0028] 2, disposable reaction bag;

[0029] 31, feeding system; 32, air inlet system; 33, temperature control system; 34, exhaust system;

[0030] 4, top cover lifting device; 41, lifting screw rod; 42, lifting motor; 43, lifting sliding block; 44, lifting support;

[0031] 5, detection electrode; 51, DO detection electrode; 52, PH value detection electrode; 53, temperature detection electrode;

[0032] 6, stirring device; 61, reactor stirring device; 611, first stirring motor; 612, first stirring shaft; 613, first stirring paddle; 62, disposable reaction bag stirring device; 621, second stirring motor; 622, second stirring shaft; 623, second stirring paddle;

[0033] 7, temperature control structure; 71, temperature control jacket; 72, avoiding groove; 73, temperature control interface;

[0034] 8, tank body support structure; 81, tank body support; 811, supporting leg. DETAILED DESCRIPTION

[0035] In order to make the objectives, characteristics and beneficial effects of the present application more obvious and easy to understand, the specific embodiments of the present application are described in detail below in combination with the drawings. It can be understood that the specific embodiments described below are only used to explain the present application, but not to limit the present application. In addition, the same or similar reference signs can be used to refer to the same or similar elements in different embodiments in the drawings, and the description of the same or similar elements in different embodiments and the description of prior art elements, characteristics, effects, etc. can be omitted. It should be noted that the axial direction, radial direction and circumferential direction described in the embodiments of the present application respectively represent the axial direction, radial direction and circumferential direction of the tank body 1.

[0036] Reference Figs. 1 to 3 The embodiments of the present application provide a multipurpose bioreactor.

[0037] Specifically, the embodiments of the present application provide a multipurpose bioreactor, which comprises a reactor body 1, the reactor body 1 comprises a tank body 11 and a top cover 12 detachably connected with the tank body 11, the tank body 11 is provided with a plurality of interface holes penetrating the wall of the tank body 11, and the outer wall of the tank body 11 is provided with an interface flange at the interface hole; a disposable reaction bag 2 can be arranged in the tank body 11 and attached to the inner wall, the disposable reaction bag 2 has a plurality of connection interfaces, and the connection interfaces are arranged one by one corresponding to the interface holes of the tank body 11; the top cover 12 is detachably provided with a fixing clamp for fixing the disposable reaction bag 2.

[0038] In a specific implementation, the interface holes at least include a feeding interface hole 111, an air inlet interface hole 112, an electrode interface hole 113 and a stirring interface hole 114; the connection interfaces at least include a feeding interface, an air inlet interface, an electrode interface and a stirring interface (not shown in the figure); the feeding interface hole 111, the air inlet interface hole 112, the electrode interface hole 113 and the stirring interface hole 114 are arranged one by one corresponding to the feeding interface, the air inlet interface, the electrode interface and the stirring interface.

[0039] In some embodiments, the feeding system 31 is connected with the interface flange at the feeding interface hole 111, and communicates with the feeding interface hole 111 or the feeding interface through the feeding interface hole 111; the gas inlet system 32 is connected with the interface flange at the gas inlet interface hole 112, and communicates with the gas inlet interface hole 112 or the gas inlet interface through the gas inlet interface hole 112; the detection electrode 5 penetrates into the electrode interface through the electrode interface hole 113 or the electrode interface hole 113, and is fixed through the interface flange at the electrode interface hole 113. In order to facilitate the feeding system 31, the gas inlet system 32 and the detection electrode 5 of the disposable reaction bag 2 to pass through the feeding interface, the gas inlet interface and the electrode interface, the feeding interface hole 111, the gas inlet interface hole 112 and the electrode interface hole 113 are usually designed to have a large diameter, and the feeding interface, the gas inlet interface and the electrode interface are designed to have a size matched with the feeding interface hole 111, the gas inlet interface hole 112 and the electrode interface hole 113 and are convenient to disassemble; in order to realize the airtight and sterile use of the gas inlet, the feeding and the installation of the detection electrode of the stainless steel reactor, the interface flange is designed to be a sterile NA flange structure, a TC chuck is used for sealing connection, the inside of the interface flange is sterile and has no dead angle, and the inner wall is smooth; the locking bolt is used in a cap nut mode to satisfy the function of locking and sealing the TC chuck and is convenient to disassemble and replace. In some embodiments, the detection electrode 5 at least includes a DO (Dissolved Oxygen, dissolved oxygen, which refers to the molecular state of oxygen dissolved in water, that is, the oxygen content in water) detection electrode 51, a PH value detection electrode 52 and a temperature detection electrode 53, and each detection electrode 5 is correspondingly provided with an electrode interface hole 113 and an interface flange.

[0040] In some embodiments, the stirring device 6 is further included, which comprises a reactor stirring device 61 and a disposable reaction bag stirring device 62. The reactor stirring device 61 is used when the disposable reaction bag 2 is not arranged in the tank body 11, penetrates the stirring interface hole 114, and is fixed through the interface flange at the stirring interface hole 114. The disposable reaction bag stirring device 62 is used when the inner wall of the tank body 11 is arranged with the disposable reaction bag 2, penetrates the stirring interface through the stirring interface hole 114, and is fixed through the interface flange at the stirring interface hole 114. The interface flange at the stirring interface hole 114 needs to install both the reactor stirring device 61 of the stainless steel reactor and the disposable reaction bag stirring device 62 of the disposable reaction bag 2. Both sealing modes and connection structures of the two stirring devices are considered in the design. An O-shaped sealing ring is arranged at the interface flange to realize the sealing of the stainless steel reactor and the reactor stirring device 61. The interface flange end face is provided with a mounting bolt hole to install the reactor stirring device 61, and a side suspension bolt is arranged to quickly install the disposable reaction bag stirring device 62. The connection between the interface flange and the tank body 11 and the bottom of the tank body 11 are flush and smooth, which ensures that the bottom of the disposable reaction bag 2 can be closely attached to the bottom of the tank body 11, realizes good temperature transfer effect and firm stirring torque conduction. In specific implementation, the stirring interface hole 114 is arranged at the bottom of the tank body 11. The reactor stirring device 61 comprises a first stirring motor 611, a first stirring shaft 612 and a first stirring paddle 613. The first stirring motor 611 is arranged outside the tank body 11. The first stirring shaft 612 penetrates the stirring interface hole 114 and extends into the tank body 11. The first stirring paddle 613 is two, and the two first stirring paddles 613 are arranged along the axial direction of the first stirring shaft 612. The disposable reaction bag stirring device 62 comprises a second stirring motor 621, a second stirring shaft 622 and a second stirring paddle 623. The second stirring motor 621 is arranged outside the tank body 11. The second stirring shaft 622 penetrates the stirring interface hole 114 and the stirring interface and extends into the disposable reaction bag 2. The second stirring paddle 623 is one, and the second stirring paddle 623 is arranged on the second stirring shaft 622.

[0041] In some embodiments, the temperature control structure 7 is further included, which comprises a temperature control jacket 71 and a heat preservation layer arranged outside the temperature control jacket 71. The temperature control jacket 71 is arranged on the outer wall of the tank body 11. The temperature control jacket 71 heats the tank body 11 by closely attaching to the outer wall of the tank body 11. The temperature control structure 7 is provided with a avoiding groove 72 at the interface flange.

[0042] In some embodiments, the temperature control system 33 is further included. The temperature control structure 7 is provided with a temperature control interface 73. The temperature control system 33 is electrically connected with the temperature control jacket 71 through the temperature control interface 73.

[0043] In some embodiments, the top cover lifting device 4 is further included, the top cover lifting device 4 is connected with the top cover 12 and can drive the top cover 12 to move, the top cover 12 can be closed on the top of the tank body 11 to close the tank body 11, the top cover lifting device 4 comprises a lifting screw 41, a lifting motor 42, a lifting sliding block 42 and a lifting support 44, the lifting sliding block 42 is arranged on the lifting screw 41, the lifting support 44 is fixed on the lifting sliding block 42 and located above the top cover 12, the lifting support 44 is connected with the top cover 12, the lifting motor 42 drives the lifting screw 41 to rotate to drive the lifting sliding block 42 and the lifting support 44 to move in the vertical direction, and then drives the top cover 12 to move.

[0044] In some embodiments, the exhaust system 34 is further included, the exhaust system 34 is connected with the top cover 12, and then communicates with the tank body 11.

[0045] In some embodiments, the tank body support structure 8 is further included, the tank body support structure 8 comprises a tank body support 81, the tank body support 81 is provided with height-adjustable supporting legs 811, and the temperature control structure 7 is arranged on the tank body support 81.

[0046] In some embodiments, the materials of the tank body 11 and the top cover 12 are stainless steel.

[0047] In the specific implementation, the electric control system is further included, the electric control system is connected with and controls the material supplementing system 31, the air inlet system 32, the temperature control system 33, the exhaust system 34, the stirring device 6 and the top cover lifting device 4, and the detection electrode 5 is connected to the electric control system 3; the electric control system 3, the material supplementing system 31, the air inlet system 32, the temperature control system 33 and the exhaust system 34 adopt the existing electric control system 3, material supplementing system 31, air inlet system 32, temperature control system 33 and exhaust system 34, and details are not repeated here.

[0048] In summary, the structure of the reactor body 1 is reasonably designed, the interface hole matched with the connection interface of the disposable reaction bag 2 is arranged on the tank body 11, the connection with the material supplementing system 31, the air inlet system 32, the detection electrode 5 and the stirring device 6 is realized through the interface hole, the use requirements of the disposable reaction bag 2 and the fixed reactor are simultaneously met, the functions of the two types of reactors are integrated, the requirements of various culture processes are met, and the equipment cost is reduced.

[0049] Further, the detachable top cover 12 is arranged, the automatic lifting of the top cover 12 is realized in cooperation with the top cover lifting device 4, the detachable fixing clamp 121 of the disposable reaction bag 2 is arranged on the top cover 12, the tank body 11 realizes the bearing of the disposable reaction bag 2, and the fixing of the top of the disposable reaction bag 2 is realized through the fixing clamp 121, and the use is more convenient.

[0050] Further, the present application can adjust the stirring device according to the use requirement by setting two groups of reactor stirring devices 61 and disposable reaction bag stirring devices 62 cooperating with different use requirements, thereby improving the production efficiency.

[0051] Although specific embodiments have been described above, these embodiments are not intended to limit the scope of the present disclosure, even if a single embodiment is described with respect to a particular feature. The feature examples provided in the present disclosure are intended to be illustrative rather than limiting, unless otherwise specified. In the implementation, one or more technical features of the dependent claims can be combined with the technical features of the independent claims in a technically feasible manner according to the actual needs, and the technical features from the corresponding independent claims can be combined by any appropriate manner rather than only by the specific combinations listed in the claims.

Claims

1. A multipurpose bioreactor characterized in that, The reactor comprises a reactor body, the reactor body comprises a tank body and a top cover detachably connected with the tank body, the tank body is provided with a plurality of interface holes penetrating the wall of the tank body, the outer wall of the tank body is provided with an interface flange at the interface hole; the interface hole comprises at least a feeding interface hole, a gas inlet interface hole, an electrode interface hole and a stirring interface hole; a disposable reaction bag can be attached to the inner wall of the tank body, the disposable reaction bag has a plurality of connection interfaces; the connection interface comprises at least a feeding interface, a gas inlet interface, an electrode interface and a stirring interface; the feeding interface hole, the gas inlet interface hole, the electrode interface hole and the stirring interface hole are arranged one by one with the feeding interface, the gas inlet interface, the electrode interface and the stirring interface; the top cover is detachably provided with a fixing clamp for fixing the disposable reaction bag; further comprising a stirring device, the stirring device comprises a reactor stirring device and a disposable reaction bag stirring device, when the tank body is not provided with a disposable reaction bag, the reactor stirring device is used, the reactor stirring device penetrates the stirring interface hole and is fixed through the interface flange at the stirring interface hole; when the tank body is provided with a disposable reaction bag attached to the inner wall, the disposable reaction bag stirring device is used, the disposable reaction bag stirring device penetrates the stirring interface through the stirring interface hole and is fixed through the interface flange at the stirring interface hole; the end surface of the interface flange at the stirring interface hole is provided with a mounting bolt hole for mounting the reactor stirring device, and the side suspension bolt is used for quickly mounting the disposable reaction bag stirring device.

2. The multipurpose bioreactor of claim 1, wherein, Further comprising a feeding system, a gas inlet system and a detection electrode, the feeding system is connected with the interface flange at the feeding interface hole, and is communicated with the feeding interface hole or the feeding interface through the feeding interface hole; the gas inlet system is connected with the interface flange at the gas inlet interface hole, and is communicated with the gas inlet interface hole or the gas inlet interface through the gas inlet interface hole; the detection electrode penetrates the electrode interface hole or the electrode interface through the electrode interface hole, and is fixed through the interface flange at the electrode interface hole.

3. The multipurpose bioreactor of claim 2, wherein, The detection electrode comprises at least a DO detection electrode, a PH value detection electrode and a temperature detection electrode.

4. The multipurpose bioreactor of claim 1, wherein, The stirring interface hole is arranged at the bottom of the tank body, the reactor stirring device comprises a first stirring motor, a first stirring shaft and a first stirring paddle, the first stirring motor is arranged outside the tank body, the first stirring shaft penetrates the stirring interface hole and extends into the tank body, the first stirring paddle is two, and the two first stirring paddles are arranged in the axial direction of the first stirring shaft; the disposable reaction bag stirring device comprises a second stirring motor, a second stirring shaft and a second stirring paddle, the second stirring motor is arranged outside the tank body, the second stirring shaft penetrates the stirring interface hole and the stirring interface and extends into the disposable reaction bag, the second stirring paddle is one, and the second stirring paddle is arranged on the second stirring shaft.

5. The multipurpose bioreactor of claim 1, wherein, The temperature control structure comprises a temperature control jacket and a heat preservation layer arranged outside the temperature control jacket, the temperature control jacket is arranged on the outer wall of the tank body, and the temperature control structure is provided with a relief groove at the interface flange.

6. The multipurpose bioreactor of claim 5, wherein, The temperature control system is electrically connected with the temperature control jacket through the temperature control interface.

7. The multipurpose bioreactor of claim 1, wherein, The top cover lifting device is connected with the top cover and can drive the top cover to move, the top cover can be closed on the top of the tank body to seal the tank body, the top cover lifting device comprises a lifting screw, a lifting motor, a lifting sliding block and a lifting support, the lifting sliding block is arranged on the lifting screw, the lifting support is fixed on the lifting sliding block and located above the top cover, and the lifting motor drives the lifting screw to rotate to drive the lifting sliding block and the lifting support to move in the vertical direction.

8. The multipurpose bioreactor of claim 1, wherein, The exhaust system is connected with the top cover.

9. The multipurpose bioreactor of claim 5, wherein, The tank body support structure comprises a tank body support, the tank body support is provided with height-adjustable supporting legs, and the temperature control structure is arranged on the tank body support.

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