Composite diatom powder live food water purifier for purifying water quality, preparation method and application

Abstract

This application discloses a composite diatom powder live food water purifier for purifying water quality, a preparation method, and applications. The composite diatom powder live food water purifier comprises, by weight, 90-95 parts of a dormant diatom strain and 5-10 parts of a support matrix. The dormant diatom strains include dormant marine strains and dormant freshwater strains. The dormant marine strains include Isochrysis, Thalassiosira wissenii, Thalassiosira pseudodwarf, Nitzschia closterium, Cyclotella cryptica, Cyclotella meni, and Phaeodactylum triangularis. The dormant freshwater strains include Achillea millefolium, Navicula, and Cylindrospermum tenuis. The support matrix comprises filter soil. The composite diatom powder live food water purifier provided in this application has the ability to withstand harsh aquatic environmental conditions, remove ammonia, and produce high oxygen, and plays an important role in improving water quality.

Description

Technical Field

[0001] The present application relates to the technical field of aquaculture preparations, and in particular to a composite diatom powder live food water purifier for purifying water quality, a preparation method and an application thereof. Background Art

[0002] Aquaculture is a vital food system, crucial for meeting the food security and nutritional needs of the world's population. Phytoplankton and algae play a crucial role in the aquatic food web, serving as the primary food source for many aquatic animals. Phytoplankton diversity is crucial for aquaculture, as it is a key element in producing high-quality, safe, and healthy aquatic products. If phytoplankton diversity is threatened, the aquaculture industry could face various challenges. Unstable water quality is a pressing challenge to the sustainable development of aquaculture. Causes of unstable water quality include excessive ammonia and other nitrogen forms in the water, low dissolved oxygen levels, anaerobic conditions caused by the sedimentation and decomposition of organic matter, and the input of external pollutants. These problems can lead to imbalances in aquatic ecosystems and compromise the profitability and productivity of aquaculture. Numerous products are currently available to address water quality issues, but each has its own advantages and disadvantages. For example, many chemical agents carry potential environmental risks and can pose potential risks to the quality and food safety of aquatic products. Although diatom products have the advantage of being safe and harmless, the liquid diatom products currently on the market have low penetration ability in deep water, short shelf life, high transportation costs, and low cell concentration, and cannot solve the problems in aquaculture.

[0003] Furthermore, many diatom products currently on the market have relatively low viability when reintroduced into water or culture medium. Although their cell shapes can be observed, they cannot survive harsh aquatic environmental conditions and wither in the water within a short period of time. This makes them unable to effectively cope with some of the problems existing in aquaculture, such as high ammonia content, low dissolved oxygen content, and the removal of nutrients (such as nitrogen, phosphorus, potassium, etc.). Summary of the Invention

[0004] In order to solve at least one of the above technical problems, a diatom strain product that is safe, efficient, highly environmentally adaptable, and has the dual functions of living food and water purification is developed. This application provides a composite diatom powder living food water purifier for purifying water quality, a preparation method and application.

[0005] In a first aspect, the present application provides a composite diatom powder live food water purifier for purifying water quality, wherein the composite diatom powder live food water purifier comprises, by weight, 90 to 95 parts of a dormant diatom strain and 5 to 10 parts of a support matrix;

[0006] The dormant diatom strains include dormant seawater strains and dormant freshwater strains;

[0007] The dormant seawater strains include Isochrysis chrysotrichum, Thalassiosira wiseri, Thalassiosira pseudodwarf, Nitzschia closterium, Cyclotella cryptica, Cyclotella meni, and Phaeodactylum triangularis.

[0008] The dormant freshwater strains include needle algae, boat algae and columnar algae;

[0009] The weight percentage of Isochrysis chrysotrichum, Thalassiosira wiseri, Thalassiosira pseudodwarf, Nitzschia crescentica, Phaeochromis triangularis, Cyclotella cryptica, Cyclotella meni, Pinella serrata, Navicula and Cylindrotheca tenuis is 1:1:1:1:1:1:1:1:1:1:1;

[0010] The support matrix comprises filter soil.

[0011] By adopting the above-mentioned technical solution, the composite diatom powder live food water purifier provided by this application has the ability to withstand harsh aquatic environmental conditions, remove ammonia, and produce high oxygen, which plays a significant role in improving water quality. Furthermore, while purifying water, the composite diatom powder live food water purifier provided by this application avoids the use of chemical reagents, reducing the impact on the environment and posing fewer concerns about food safety and product quality.

[0012] Among them, dormant diatom strains have the functions of adsorption and filtration, which can remove suspended matter, particulate matter and organic matter in the water. At the same time, they also provide a suitable living environment for phytoplankton and microorganisms in the water body, promoting their growth and reproduction. Dormant diatom cells can adapt to adverse environments such as low oxygen, high salt, and high temperature, and maintain their activity. Dormant diatom cells can adsorb ammonia ions in the water to the cell surface through adsorption and absorption, thereby removing ammonia. This has a certain repair effect on ammonia pollution in water bodies. In addition, dormant diatom cells produce oxygen during photosynthesis, which leads to an increase in the dissolved oxygen concentration in the water body. High dissolved oxygen helps maintain the ecological balance of the water body and promotes the growth and reproduction of aquatic organisms.

[0013] The supporting matrix provides stability and nutritional supplements, allowing the compound diatom powder live food water purifier to continue to play a purifying role in the water body.

[0014] Therefore, the composite diatom powder live food water purifier provided in this application is an innovative diatom strain product with the dual functions of live food and water purification. The composite diatom powder live food water purifier selects diatom strains with strong adaptability and optimizes their cultivation to ensure their stable growth and reproduction in various water bodies. It not only provides rich and nutritious live food for the aquatic food web, but also efficiently purifies the water quality. The live food function of the diatom powder water purifier makes it an ideal choice for aquatic food webs. Diatom strains are rich in nutrients such as protein, vitamins and minerals, which can meet the needs of aquatic organisms and promote their healthy growth. At the same time, diatom strains have a high degree of environmental adaptability and can adapt to the different temperatures, salinity and pH values ​​of various water bodies, giving them wide application potential in aquaculture or aquatic ecosystems. In addition to its live food function, the diatom powder water purifier also plays an important role in purifying water quality. Through photosynthesis and adsorption, diatom strains can effectively remove pollutants such as organic matter, suspended matter and heavy metals in water, thereby improving the cleanliness and transparency of the water. They can regulate the nutrient content in the water, reduce the occurrence of algae red tides, and promote the growth of other beneficial microorganisms in the water, forming a virtuous cycle of the ecosystem. The safety and efficiency of the compound diatom powder live food water purifier are due to the strict screening and cultivation of diatom strains to ensure that they do not contain toxic substances and pathogenic microorganisms. It does not use any chemical agents, has no pollution to the water quality and ecological environment, meets environmental protection requirements, and provides a reliable option for sustainable aquaculture. In short, as a diatom strain product with the dual functions of living food and water purification, diatom powder water purifier will bring innovative solutions to the aquatic food web and water purification field, and achieve the sustainable development of aquaculture and the maintenance of a healthy water environment.

[0015] Optionally, the average particle size of the composite diatom powder live food water purifier is 2.36 mm.

[0016] By adopting the technical solution, the composite diatom powder live food water purifier with a particle size of 2.36 mm provided by this application can solve the live food and water purification needs of the aquatic food web. The product has a composite diatom powder with an average particle size of 2.36 mm, which can be stably dispersed in water bodies and provide rich nutrition for aquatic organisms. The composite diatom powder live food water purifier with a particle size of 2.36 mm has dual functions. First, as a live food, the composite diatom powder is rich in nutrients such as protein, vitamins and minerals, which can meet the growth needs of aquatic organisms and promote their healthy development. Its particle size is adapted to the mouth structure and feeding habits of different aquatic organisms, and is easy to be swallowed and digested. Secondly, the composite diatom powder live food water purifier with a particle size of 2.36 mm has the ability to purify water quality. Through photosynthesis and adsorption, the composite diatom powder can efficiently remove pollutants such as organic matter, suspended matter and heavy metals in water, thereby improving the clarity and transparency of the water quality. They also regulate the nutrient content in the water, reduce the occurrence of algae red tides, and promote the reproduction of other beneficial microorganisms to maintain the balance of the aquatic ecosystem. The technical solution of the composite diatom powder live food water purifier with a particle size of 2.36mm ensures its safety, high efficiency, and environmental adaptability. The product is free of toxic substances and pathogenic microorganisms, does not use chemical agents, and meets environmental protection requirements. By using this product, the sustainable development of aquaculture can be achieved and the health of the water environment can be maintained. In summary, the 2.36mm composite diatom powder live food water purifier is a product with the dual functions of live food and water purification, and the needs of the aquatic food network are solved through the said technical solution.

[0017] Preferably, the diatom powder water purifier comprises 95 parts of dormant diatom strains and 5 parts of supporting matrix by weight.

[0018] Optionally, the diatom powder water purifier further comprises water, and the percentage of water is not higher than 12%.

[0019] In a second aspect, the present application provides an application of the composite diatom powder live food water purifier for purifying water quality described in the present application in the field of aquaculture.

[0020] By adopting the technical solution, the composite diatom powder live food water purifier solution provided in this application can be used in the field of aquaculture to purify water quality and ensure the cleanliness and health of water bodies. This water purifier has the ability to efficiently remove pollutants such as organic matter, suspended matter and heavy metals, and improves the transparency and cleanliness of water quality through photosynthesis and adsorption. In addition, the composite diatom powder live food water purifier can also regulate the nutrient content in water, reduce the occurrence of algal red tides, and promote the growth of other beneficial microorganisms, maintain the balance of the water ecosystem, and improve the stability and sustainability of the aquaculture environment. In addition, the composite diatom powder live food water purifier is a safe, efficient and environmentally friendly product. It does not contain toxic substances and pathogenic microorganisms and does not require the use of any chemical agents. Therefore, the application of this water purifier in aquaculture can effectively improve water quality, protect the health of aquatic organisms and increase aquaculture benefits. In summary, the composite diatom powder live food water purifier has the advantages of being used in the field of aquaculture for water purification, can ensure the cleanliness and health of water bodies, improve aquaculture benefits, and meet environmental protection requirements.

[0021] Optional, 666.67m 2 200g of the composite diatom powder live food water purifier for purifying water quality described in this application is used for paddy fields.

[0022] By adopting the above-mentioned technical solution, the present application can address the high cost issue of traditional water purifiers. Compared to traditional water purifiers, the composite diatom powder live food water purifier technical solution provided by this application is more cost-effective. First, this solution utilizes diatom powder, a natural, renewable purification material, which is less expensive than synthetic chemicals. Furthermore, diatom powder can cultivate dormant beneficial algae to achieve autonomous reproduction and water purification, eliminating the need for additional preparation and processing, further reducing the cost of the water purifier. In addition to its cost advantage, the technical solution provided by this application is also environmentally friendly. Using the composite diatom powder live food water purifier for water purification eliminates the need for any chemical agents, avoiding environmental pollution and harm to aquatic life. Compared to traditional water purifiers, the purification process of this solution is more natural and bio-friendly. In summary, the composite diatom powder live food water purifier technical solution provided by this application can address the high cost issue of traditional water purifiers. Its cost-effectiveness and environmental friendliness give it certain advantages in the field of water purification.

[0023] In a third aspect, the present application provides a method for preparing the composite diatom powder live food water purifier for purifying water quality, the preparation method comprising a cultivation process, a harvesting process, a freeze-drying process, and a final product processing process;

[0024] S1. Cultivation process

[0025] The culturing process includes a culturing process for seed and pilot-scale diatom strains and a culturing process for large-scale diatom strains. The culturing process for the seed and pilot-scale diatom strains is as follows: a single seawater strain is cultured in an F / 2+Si medium for activation culture, a single freshwater strain is cultured in a CSI medium for activation culture, and then incubated in a photobioreactor. The incubation conditions are as follows: a light intensity of 2000 to 2500 lux, a light incubator temperature of 25±2° C., and a light / dark cycle ratio of 14:10 hours.

[0026] The large-scale cultivation process of the diatom strain is as follows: the diatom strain is added to the F / 2+Si culture medium according to the weight ratio to form a symbiotic diatom, the culture is activated, and then incubated in a photobioreactor. The incubation conditions are as follows: the light intensity is 3000 to 6000 lux, the temperature of the light incubator is 25±2°C, the continuous lighting time is not less than 3 days, air is injected, and the pH is controlled at 7.0±2. At the end of the culture, the air supply is stopped;

[0027] S2. The harvesting process is as follows:

[0028] After the incubation, the diatom strain was settled to solid-liquid separation, the supernatant was removed, and the precipitated diatom material was collected;

[0029] S3, freeze drying process:

[0030] Mixing the diatom material collected in step S2 with 0.1% by weight of cactus mucus and 3% by weight of filtered black soil to prepare a pre-diatom product, and then freeze-drying the pre-diatom product to obtain a freeze-dried pre-diatom product;

[0031] S4. Final product processing:

[0032] The revival rate, moisture content, and heavy metal content of the freeze-dried pre-diatom product obtained in step S3 are analyzed to prepare a composite diatom powder live food water purifier for purifying water quality.

[0033] By adopting the technical solution, the preparation method of the composite diatom powder live food water purifier provided by the present application has the advantages of high efficiency, simplicity, strong controllability and good comprehensive purification effect, and is expected to be widely used in the field of water purification. Specifically embodied in the following aspects, firstly, the preparation method of the composite diatom powder live food water purifier provided by the present application, by using composite diatom powder as a water purifier, has high adsorption capacity and activity, can efficiently remove pollutants such as suspended matter, particulate matter, organic matter, heavy metal ions in water, effectively purify water quality, and improve water quality. Secondly, the preparation method includes a cultivation process, a harvesting process, a freeze-drying process and a final product processing process, and each step is relatively simple and easy to operate and control. Thirdly, the raw materials required for the preparation method include diatom strains, cactus mucus and black soil, which are relatively common and easy to obtain, which is conducive to large-scale production and application. Fourthly, during the cultivation process, the growth and activity of the diatom strain can be regulated by regulating the cultivation conditions, such as light intensity, temperature, light / dark cycle ratio and other parameters, thereby achieving the regulation and optimization of the performance of the final product. Fifthly, the composite diatom powder live food water purifier has a comprehensive effect in purifying water quality. It can remove a variety of pollutants such as suspended matter, particulate matter, organic matter, heavy metals, etc., improve the cleanliness and taste of water, and thus provide people with safer and healthier aquatic products. In addition, the composite diatom powder live food water purifier can also provide food for aquatic organisms, including algae, zooplankton, etc., thereby promoting their growth and reproduction and maintaining a good balance in the aquatic ecosystem. The nutrients in the composite diatom powder can provide the nutrients needed for the reproduction of aquatic organisms, increase the population size and diversity, and maintain ecological balance and species diversity. By providing food and nutritional supplements, the composite diatom powder live food water purifier can also improve the ecological environment and improve the biological performance and ecological sustainability of water quality.

[0034] Preferably, the F / 2+Si medium used in the large-scale cultivation of diatom strains includes the following components by weight concentration: ammonium sulfate is 100 mg / L, urea is 75 mg / L, NPK is 15 mg / L, sterilized NaNO3 is 15 mg / L, (NH4)(H2PO4) is 15 mg / L, sterilized Na2HPO4·2H2O is 0.0057 g / L, Na2SiO3.9H2O is 0.03 g / L, vitamins are 1 ml / L, vitamin B 12 is 0.0005g / L, vitamin B6 is 0.1g / L, biotin is 0.0005g / L, Na2EDTA·2H2O is 4.36g / L, FeCl3·6H2O is 3.15g / L, MnCl2·4H2O is 0.18g / L, ZnSo4.7H2O is 0.022g / L, CoCl2·6H2O is 0.01g / L, and Na2MoO4·2H2O is 0.006g / L.

[0035] This application realizes the large-scale production of composite diatom powder live food water purifier by optimizing the ingredients of F / 2+Si culture medium. The optimized culture medium contains a variety of organic nutrients, inorganic salts and trace elements to meet the growth requirements of diatom strains. Optimizing the ratio helps to increase the growth rate and biomass yield of diatom strains. The F / 2+Si culture medium with optimized ingredients is suitable for the cultivation of a variety of diatom strains, improving the adaptability and purification effect of the product. The ingredients are relatively simple and clear, easy to obtain, and meet the needs of large-scale production. By optimizing the ratio of ingredients, the concentration of nutrients in the culture medium can be further adjusted, and the growth conditions and yield can be optimized. This helps to meet the needs of large-scale and efficient production in the field of water purification, and improves the feasibility and market competitiveness of the product.

[0036] Optionally, the freeze-drying process in step S3 includes, in order, an automatic freeze-drying process and a manual freeze-drying process;

[0037] The freeze-drying automatic processing parameters are as follows: the computer program is set to freeze-drying automatic mode and freeze-drying is started; the freeze-drying parameters are as follows: starting temperature is -40°C, pressure is 0.01 MPa, duration is 30 minutes, and sublimation freeze-drying is performed at a temperature increase of 5°C every 30 minutes until the temperature reaches 20°C;

[0038] The freeze-drying manual processing parameters are as follows: when the temperature rises to 20°C, adjust the computer program to freeze-drying manual, turn on the air dehumidifier, set the temperature to 25°C and the humidity to 30%, and wait until the freeze-drying box has no negative pressure, and then prepare to receive the material.

[0039] In a fourth aspect, the present application provides a method for resuscitating the composite diatom powder live food water purifier prepared by the above preparation method, the resuscitation method comprising the following steps:

[0040] The composite diatom powder live food water purifier can be put into a water body or a culture medium to realize the resuscitation of the composite diatom powder live food water purifier.

[0041] By adopting the above technical solution, the composite diatom powder live food water purifier obtained by the preparation method provided in this application is easy to use and can be revived by simply placing it in water or culture medium. The revived composite diatom powder water purifier can exert the effects of the diatom strains to purify and improve water quality.

[0042] In summary, the present invention includes at least one of the following beneficial technical effects:

[0043] 1. The composite diatom powder live food water purifier provided in this application is an innovative diatom strain product with the dual functions of live food and water purification. The composite diatom powder live food water purifier selects diatom strains with strong adaptability and optimizes their cultivation to ensure that they can grow and reproduce stably in various water bodies. It not only provides rich and nutritious live food for the aquatic food web, but also efficiently purifies the water quality. The live food function of the diatom powder water purifier makes it an ideal choice for aquatic food webs. Diatom strains are rich in nutrients such as protein, vitamins and minerals, which can meet the needs of aquatic organisms and promote their healthy growth. At the same time, diatom strains have a high degree of environmental adaptability and can adapt to the different temperatures, salinity and pH values ​​of various water bodies, giving them wide application potential in aquaculture or aquatic ecosystems. In addition to the live food function, the diatom powder water purifier also plays an important role in purifying water quality. Through photosynthesis and adsorption, diatom strains can effectively remove pollutants such as organic matter, suspended matter and heavy metals in water, thereby improving the cleanliness and transparency of the water. They can regulate nutrient levels in water, reduce the occurrence of algal blooms, and promote the growth of other beneficial microorganisms in the water, forming a virtuous cycle of ecosystems. The safety and effectiveness of the compound diatom powder live food water purifier are due to the rigorous screening and cultivation of diatom strains to ensure they are free of toxic substances and pathogenic microorganisms. It does not use any chemical agents, is harmless to water quality and the ecological environment, and meets environmental protection requirements, providing a reliable option for sustainable aquaculture.

[0044] 2. The composite diatom powder live food water purifier provided in this application has the advantage of being used for water purification in the field of aquaculture, which can ensure the cleanliness and health of water bodies, improve aquaculture efficiency, and meet environmental protection requirements.

[0045] 3. The preparation method of the composite diatom powder live food water purifier provided in this application has the advantages of high efficiency, simplicity, strong controllability and good comprehensive purification effect, and is expected to be widely used in the field of water purification.

[0046] 4. The composite diatom powder live food water purifier obtained by the preparation method provided herein is easy to use and can be revived by simply placing it in water or culture medium. The revived composite diatom powder water purifier can utilize the diatom strains to purify and improve water quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] Figure 1 The water purifier obtained in Example 1;

[0048] Figure 2 The resurrection of the water purifier obtained from Example 1 in water after 60 months. DETAILED DESCRIPTION

[0049] The present application is further described in detail below with reference to the accompanying drawings and examples.

[0050] In a first aspect, the present application provides a composite diatom powder live food water purifier for purifying water quality, wherein the composite diatom powder live food water purifier comprises 90-95 parts of dormant diatom strains and 5-10 parts of a support matrix in parts by weight;

[0051] The dormant diatom strains include dormant seawater strains and dormant freshwater strains;

[0052] The dormant seawater strains include Isochrysis chrysotrichum, Thalassiosira wiseri, Thalassiosira pseudodwarf, Nitzschia closterium, Cyclotella cryptica, Cyclotella meni, and Phaeodactylum triangularis.

[0053] The dormant freshwater strains include needle algae, boat algae and columnar algae;

[0054] The weight percentage of Isochrysis chrysotrichum, Thalassiosira wiseri, Thalassiosira pseudodwarf, Nitzschia crescentica, Phaeochromis triangularis, Cyclotella cryptica, Cyclotella meni, Pinella serrata, Navicula and Cylindrotheca tenuis is 1:1:1:1:1:1:1:1:1:1:1;

[0055] The support matrix comprises filter soil.

[0056] In a second aspect, the present application provides an application of the composite diatom powder live food water purifier for purifying water quality described in the present application in the field of aquaculture.

[0057] In a third aspect, the present application provides a method for preparing the composite diatom powder live food water purifier for purifying water quality, the preparation method comprising a cultivation process, a harvesting process, a freeze-drying process, and a final product processing process;

[0058] S1. Cultivation process

[0059] The culturing process includes a culturing process for seed and pilot-scale diatom strains and a culturing process for large-scale diatom strains. The culturing process for the seed and pilot-scale diatom strains is as follows: a single seawater strain is cultured in an F / 2+Si medium for activation culture, a single freshwater strain is cultured in a CSI medium for activation culture, and then incubated in a photobioreactor. The incubation conditions are as follows: a light intensity of 2000 to 2500 lux, a light incubator temperature of 25±2° C., and a light / dark cycle ratio of 14:10 hours.

[0060] The large-scale cultivation process of the diatom strain is as follows: the diatom strain is added to the F / 2+Si culture medium according to the weight ratio to form a symbiotic diatom, the culture is activated, and then incubated in a photobioreactor. The incubation conditions are as follows: the light intensity is 3000 to 6000 lux, the temperature of the light incubator is 25±2°C, the continuous lighting time is not less than 3 days, air is injected, and the pH is controlled at 7.0±2. At the end of the culture, the air supply is stopped;

[0061] S2. The harvesting process is as follows:

[0062] After the incubation, the diatom strain was settled to solid-liquid separation, the supernatant was removed, and the precipitated diatom material was collected;

[0063] S3, freeze drying process:

[0064] Mixing the diatom material collected in step S2 with 0.1% by weight of cactus mucus and 3% by weight of filtered black soil to prepare a pre-diatom product, and then freeze-drying the pre-diatom product to obtain a freeze-dried pre-diatom product;

[0065] S4. Final product processing:

[0066] The revival rate, moisture content, and heavy metal content of the freeze-dried pre-diatom product obtained in step S3 are analyzed to prepare a composite diatom powder live food water purifier for purifying water quality.

[0067] While researching water purifiers, the inventors discovered that currently used chemical water purifiers are unsafe, inefficient, and costly. For example, chemical water purifiers may contain various chemicals, such as chlorine, bromine, and fluorine. The disinfection byproducts formed by these substances in water may have a negative impact on human health. In addition, some ingredients in chemical water purifiers may cause secondary pollution to the water quality. For example, added agents may affect the taste, odor, and color of the water, making it unpalatable. The long-term use of chemical water purifiers may cause the accumulation of harmful substances in the water quality, with potential long-term side effects on human health. In addition, the use of chemical water purifiers may lead to the discharge of harmful substances in wastewater, polluting the environment.

[0068] In comparison, diatom water purifiers are safe, but current diatom products have poor weather resistance, and liquid diatom products cannot fully sink to the bottom of the water, resulting in lower water purification effects.

[0069] To address the high costs, unsafe ingredients, and low water purification efficiency issues of existing technologies, the inventors have conducted research and, in this application, provide a safe, efficient, environmentally adaptable composite diatom powder live food water purifier for purifying water. The composite diatom powder live food water purifier comprises, by weight, 90-95 parts of a dormant diatom strain and 5-10 parts of a support matrix.

[0070] The dormant diatom strains include dormant seawater strains and dormant freshwater strains;

[0071] The dormant seawater strains include Isochrysis chrysotrichum, Thalassiosira wiseri, Thalassiosira pseudodwarf, Nitzschia closterium, Cyclotella cryptica, Cyclotella meni, and Phaeodactylum triangularis.

[0072] The dormant freshwater strains include needle algae, boat algae and columnar algae;

[0073] The weight percentage of Isochrysis chrysotrichum, Thalassiosira wiseri, Thalassiosira pseudodwarf, Nitzschia crescentica, Phaeochromis triangularis, Cyclotella cryptica, Cyclotella meni, Pinella serrata, Navicula and Cylindrotheca tenuis is 1:1:1:1:1:1:1:1:1:1:1;

[0074] The support matrix comprises filter soil.

[0075] By adopting the above-mentioned technical solution, the composite diatom powder live food water purifier provided by this application has the ability to withstand harsh aquatic environmental conditions, remove ammonia, and produce high oxygen, which plays a significant role in improving water quality. Furthermore, while purifying water, the composite diatom powder live food water purifier provided by this application avoids the use of chemical reagents, reducing the impact on the environment and posing fewer concerns about food safety and product quality. Specific embodiments

[0077] The raw materials used are commercially available products unless otherwise specified.

[0078]

[0079] Examples 1-5: Cultivation of Seed and Pilot-Scale Diatom Strains

[0080] Examples 1 to 5 provide a composite diatom powder live food water purifier for purifying water quality and a preparation method.

[0081] Example 1

[0082] This embodiment provides a composite diatom powder live food water purifier for purifying water with a particle size of 2.36 mm, which comprises 90 parts of dormant diatom strains and 10 parts of filtered black soil by weight;

[0083] The dormant diatom strains include dormant seawater strains and dormant freshwater strains;

[0084] The dormant seawater strains include Isochrysis chrysotrichum, Thalassiosira wiseri, Thalassiosira pseudodwarf, Nitzschia closterium, Cyclotella cryptica, Cyclotella meni, and Phaeodactylum triangularis.

[0085] The dormant freshwater strains include needle algae, boat algae and columnar algae;

[0086] The weight percentage of Isochrysis chrysotrichum, Thalassiosira wiseri, Thalassiosira pseudodwarf, Nitzschia crescentica, Pseudo-triangular finger algae, Cyclotella cryptica, Cyclotella meni, Needle algae, Navicula, and Cylindrotheca tenuis is 1:1:1:1:1:1:1:1:1:1:1;

[0087] The support matrix comprises filter soil.

[0088] The preparation method of a composite diatom powder live food water purifier for purifying water provided in this embodiment includes the following steps: S1, culturing process: activating a single seawater strain in F / 2+Si medium and activating a single freshwater strain in CSI medium, and then incubating them in a photobioreactor under the following incubation conditions: light intensity of 2000 lux, light incubator temperature of 25±2°C, and light / dark cycle ratio of 14:10 hours;

[0089] S2. The harvesting process is as follows:

[0090] After the incubation, the diatom strain was settled until solid-liquid separation occurred, the supernatant was removed, and the precipitated diatom material was collected;

[0091] S3, freeze drying process:

[0092] The 10 different diatom materials collected in step S2 are mixed in equal amounts with 0.1% by weight of cactus mucus and filtered black soil to prepare a pre-diatom product, and then the pre-diatom product is freeze-dried to obtain a freeze-dried pre-diatom product;

[0093] The parameters for the automatic freeze-drying process are as follows: the computer program is set to automatic freeze-drying and freeze-drying is started; the minimum temperature is -40°C, the pressure is 0.01 MPa, the duration is 30 minutes, and sublimation freeze-drying is performed at a rate of 5°C every 30 minutes until the temperature reaches 20°C; the parameters for the manual freeze-drying process are as follows: after the temperature rises to 20°C, the computer program is set to manual freeze-drying, the air dehumidifier is turned on, the temperature is set to 25°C, and the humidity is set to 30%, and when the freeze-drying chamber has no negative pressure, it is ready to receive the material.

[0094] S4. Final product processing:

[0095] The freeze-dried pre-diatom product obtained in step S3 was analyzed for its resurrection rate, moisture content, and heavy metal content to produce a composite diatom powder live food water purifier for purifying water. The differences between Examples 2 and 3 and Example 1 are shown in Table 1.

[0096] Table 1 - The differences between Examples 2 to 3 and Example 1 are shown in Table

[0097]

[0098]

[0099] Example 4

[0100] The difference between this embodiment and embodiment 3 is that some parameters in step S1 of this embodiment are different from those in embodiment 3.

[0101] S1. Cultivation process

[0102] Individual seawater strains were activated in F / 2+Si medium, and individual freshwater strains were activated in CSI medium, and then incubated in a photobioreactor. The incubation conditions were as follows: light intensity of 2200 lux, light incubator temperature of 25±2℃, and light / dark cycle ratio of 14:10 hours.

[0103] Example 5

[0104] The difference between this embodiment and embodiment 3 is that some parameters in step S1 of this embodiment are different from those in embodiment 3.

[0105] S1. Cultivation process

[0106] Individual seawater strains were activated in F / 2+Si medium, and individual freshwater strains were activated in CSI medium, and then incubated in a photobioreactor. The incubation conditions were as follows: light intensity of 2500 lux, light incubator temperature of 25±2℃, and light / dark cycle ratio of 14:10 h.

[0107] Through experiments in Examples 1 to 5, the inventors found that the culture method described in step S1 provided in Examples 1 to 5 is suitable for the cultivation of seed and pilot-scale diatom strains. Therefore, based on the culture process in Example 3, the inventors provide a culture process suitable for large-scale diatom strain cultivation in Example 6.

[0108] Examples 6-9: Large-scale cultivation of diatom strains

[0109] Example 6

[0110] The difference between this embodiment and embodiment 3 is that the culture process of step S1 in this embodiment is as follows:

[0111] S1. Cultivation process

[0112] The cultivation process of large-scale diatom strains is as follows:

[0113] The diatom strain was added to the F / 2+Si culture medium in a weight ratio to form a symbiotic diatom, the culture was activated, and then the culture was incubated in a photobioreactor. The incubation conditions were as follows: light intensity of 3000 lux, light incubator temperature of 25±2°C, continuous lighting time of 3 days, air was injected, and the pH was controlled at 7.0±2. At the end of the culture, the air supply was stopped.

[0114] Example 7

[0115] The difference between this embodiment and embodiment 6 is that the light intensity in step S1 is 5000 lux.

[0116] Example 8

[0117] The difference between this embodiment and embodiment 6 is that the light intensity in step S1 is 6000 lux.

[0118] Example 9

[0119] The difference between this embodiment and embodiment 8 is that the inventors further optimized the components of the F / 2+Si medium in this embodiment. The F / 2+Si medium includes the following components by weight concentration: ammonium sulfate is 100 mg / L, urea is 75 mg / L, NPK is 15 mg / L, sterilized NaNO3 is 15 mg / L, (NH4)(H2PO4) is 15 mg / L, sterilized Na2HPO4·2H2O is 0.0057 g / L, and Na2SiO3.9H2O is 0.03 g / L, vitamin 1ml / L, vitamin B12 is 0.0005g / L, vitamin B6 is 0.1g / L, biotin is 0.0005g / L, Na2EDTA·2H2O is 4.36g / L, FeCl3·6H2O is 3.15g / L, MnCl2·4H2O is 0.18g / L, ZnSo4.7H2O is 0.022g / L, CoCl2·6H2O is 0.01g / L, and Na2MoO4·2H2O is 0.006g / L.

[0120] Comparative Examples 1 to 12

[0121] Comparative Example 1

[0122] The difference between this comparative example and Example 1 is that an equal amount of Distichus spp. is used in this comparative example to replace the Navicula algae strain.

[0123] Comparative Example 2

[0124] The difference between this comparative example and Example 1 is that this comparative example uses an equal amount of Discoidea to replace the Isochrysis sp. strain.

[0125] Comparative Example 3

[0126] The difference between this comparative example and Example 1 is that an equal amount of Discoideum spp. is used in this comparative example to replace the Thalassiosira wiseri strain.

[0127] Comparative Example 4

[0128] The difference between this comparative example and Example 1 is that an equal amount of Discoideum spp. is used in this comparative example to replace the Thalassiosira pseudonana strain.

[0129] Comparative Example 5

[0130] The difference between this comparative example and Example 1 is that an equal amount of the Dictyophora discoides strain is used in this comparative example to replace the Nitzschia closterium strain.

[0131] Comparative Example 6

[0132] The difference between this comparative example and Example 1 is that an equal amount of the disc-shaped algae is used to replace the triangular algae strain.

[0133] Comparative Example 7

[0134] The difference between this comparative example and Example 1 is that an equal amount of the Cyclotella cryptica strain is used in this comparative example.

[0135] Comparative Example 8

[0136] The difference between this comparative example and Example 1 is that an equal amount of the Cyclotella menifolia strain is used in this comparative example.

[0137] Comparative Example 9

[0138] The difference between this comparative example and Example 1 is that an equal amount of Discoidea algae is used in this comparative example to replace the Acanthopanax strain.

[0139] Comparative Example 10

[0140] The difference between this comparative example and Example 1 is that an equal amount of Discoidas algae is used in this comparative example to replace the Cylindrotheca tenuissima strain.

[0141] Comparative Example 11

[0142] The difference between this comparative example and Example 1 is that this example uses a liquid water purifier purchased from a powerful supplier of Guangzhou Zhengge Agriculture and Animal Husbandry, model number: Shuiyinmei.

[0143] Comparative Example 12

[0144] The difference between this comparative example and Example 1 is that this comparative example uses a commercially available water purifier purchased from Liaocheng Youyu Aquarium Products Co., Ltd., a powerful supplier, with a product number of 650ml.

[0145] Performance test experiment 1,

[0146] Resurrection rate, moisture content, and heavy metal content detection

[0147] In this experiment, 1000 g of the water purifier obtained in Examples 1 to 9 was taken to measure the water content and heavy metal content of the water purifier. The test results are shown in Table 2.

[0148] The moisture content is determined according to the method for determination of moisture in feeds in GB / T 6435.

[0149] Heavy metal content testing includes the determination of lead, cadmium, mercury and arsenic.

[0150] The determination of lead metal shall refer to the determination method of GB / T 10380.

[0151] The determination of cadmium metal shall refer to the determination method of GB / T 10382.

[0152] The determination of mercury metal shall refer to the determination method of GB / T 10381.

[0153] The determination of arsenic metal shall refer to the determination method of GB / T 10379.

[0154] The lead metal content in the composite diatom powder live food water purifier is not higher than 5.0 mg / Kg, the cadmium metal content is not higher than 0.5 mg / Kg, the mercury content is not higher than 0.1 mg / Kg, and the arsenic content is not higher than 2.0 mg / Kg.

[0155] Table 2 - Test results of Experiment 1

[0156]

[0157] Performance test experiment 2

[0158] In this experiment, 21 groups of 100L fish tanks were prepared, and an equal amount of water from a fish and shrimp pond in Xiamen was placed in each fish tank. The water was turbid, light gray, with floating objects on the water surface and a fishy smell. 100 yellow croaker fry with a length of about 10 cm were also placed in each fish tank. 20g of the water purifier prepared by Examples 1 to 9 and Comparative Examples 1 to 12 was added to each fish tank every day. In addition, 20g of fish feed was added to the fish tank of Comparative Examples 11 to 12 every day. Adequate oxygen supply was maintained throughout the test. The growth of the fry was observed and recorded at the end of Week 0 and Week 4, with a total observation period of four weeks. See Tables 3 to 4 for the test results.

[0159] In this experiment, in addition to detecting the growth of fry, the inventors also measured the conditions of the above water bodies at week 0 and at the end of the experiment in accordance with the sub-class water body detection standards in the "National Standard of the People's Republic of China" GB3838-2002. The experimental results are shown in Tables 3 and 4.

[0160] Table 3 - Summary of the detection results of Examples 1 to 9 in Week 0

[0161]

[0162] Table 4 - Summary of the test results of Examples 1 to 9 in the 4th week

[0163]

[0164]

[0165] Table 5 - Summary of the test results of comparative examples 1 to 12 in week 0

[0166]

[0167]

[0168] Table 6 - Summary of the test results of comparative examples 1 to 12 in the 4th week

[0169]

[0170] Result analysis:

[0171] As shown in the test results of Tables 3 to 6, the composite diatom powder live food water purifier for purifying water prepared by the present application has obvious advantages over the water purifier obtained in the comparative example in terms of both water purification and increasing fish weight.

[0172] The difference between Examples 2 to 3 and Example 1 is that in Examples 2 to 3, the weight proportions of the dormant diatom strains and the filtered black soil are different. From the test results provided in Tables 3 to 6, it can be seen that the composite diatom powder live food water purifier obtained by Example 3 has a better effect in purifying water quality.

[0173] The difference between Examples 4 to 5 and Example 3 is that the preparation parameters are different.

[0174] Example 6 is a method for cultivating large-scale diatom strains. Compared with the preparation method of Example 5, the preparation method of Example 6 has the advantage of simple operation, but the water purification effect and the increase in fish weight are slightly lower than Example 5. The reason may be that the preparation parameters and culture medium of Example 6 need to be further optimized. Therefore, the inventor further optimized Example 6 based on Example 6. The difference between Examples 7 and 8 and Example 6 is that the preparation parameters are different. Obviously, the preparation method of Example 8 is more effective. Therefore, the inventor further optimized the composition of the culture medium based on Example 8. The test results show that the composite diatom powder live food water purifier for purifying water quality prepared in Example 9 has the advantage of better water purification effect.

[0175] Comparative Examples 1-10 differ from Example 1 in that the composition of one of the diatom strains is different. The test results indicate that the composite diatom powder live food water purifier prepared by the present application is more effective in purifying water. This may be because the water purifier prepared by the present application can quickly adapt to various polluted water environments.

[0176] The difference between Comparative Example 11 and Example 1 is that the water purification effect of the liquid water purifier obtained in Comparative Example 11 is not good. The reason may be that the liquid water purifier may float on the water surface, thereby making it impossible to purify the bottom of the water pollutants.

[0177] The difference between Comparative Example 12 and Example 1 is that Comparative Example 12 is a commercially available water purifier. The water purifier obtained in Comparative Example 12 not only contains chemical components that pollute the water body, but also has a poor water purification effect.

[0178] Performance test 3, shelf life test

[0179] The water purifiers obtained in Examples 1 to 9 of the present application exist in the form of powder in the dormant state. The shape of the composite diatom powder live food water purifier for purifying water obtained in Example 1 is shown in FIG. Figure 1 .

[0180] The water purifiers obtained in Examples 1 to 9 were placed in a room temperature environment and tested for resuscitation in water after 12 months, 24 months, 36 months, 48 ​​months, 60 months, and 72 months. The test results showed that the water purifiers obtained in Examples 1 to 9 of the present application had an effective period of 60 months and a resuscitation rate of over 90%.

[0181] The resuscitation of the water purifier obtained from Example 1 in water at 60 months is shown in FIG. Figure 2 .

[0182] The above are all preferred embodiments of the present application, and are not intended to limit the scope of protection of the present application. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be included in the scope of protection of the present application.

Claims

1. A composite diatom powder live food water purifier for purifying water quality, characterized in that: The composite diatom powder live food water purifier comprises 90-95 parts of dormant diatom strains and 5-10 parts of support matrix in parts by weight; The dormant diatom strains include dormant seawater strains and dormant freshwater strains; The dormant seawater strains include Isochrysis chrysotrichum, Thalassiosira wiseri, Thalassiosira pseudodwarf, Nitzschia closterium, Cyclotella cryptica, Cyclotella meni, and Phaeodactylum triangularis. The dormant freshwater strains include needle algae, boat algae and columnar algae; The weight percentage of Isochrysis chrysotrichum, Thalassiosira wiseri, Thalassiosira pseudodwarf, Nitzschia crescentica, Phaeochromis triangularis, Cyclotella cryptica, Cyclotella meni, Pinella serrata, Navicula and Cylindrotheca tenuis is 1:1:1:1:1:1:1:1:1:1:1; The support matrix comprises filter soil.

2. The composite diatom powder live food water purifier for purifying water according to claim 1, characterized in that: The average particle size of the composite diatom powder live food water purifier is 2.36 mm.

3. The composite diatom powder live food water purifier for purifying water according to claim 1, characterized in that: The composite diatom powder live food water purifier comprises 95 parts of dormant diatom strains and 5 parts of supporting matrix by weight.

4. The composite diatom powder live food water purifier for purifying water according to claim 1, characterized in that: The composite diatom powder live food water purifier also includes water, and the percentage of water is not higher than 12%.

5. Use of the composite diatom powder live food water purifier for purifying water quality according to claim 1 in the field of aquaculture.

6. The use of the composite diatom powder live food water purifier for purifying water quality according to claim 5 in the field of aquaculture, characterized in that: 666.67m 2 200g of the composite diatom powder live food water purifier for purifying water quality in paddy fields.

7. A method for preparing a composite diatom powder live food water purifier for purifying water according to claim 1, characterized in that: The preparation method includes a cultivation process, a harvesting process, a freeze-drying process, and a final product processing process; S1. Cultivation process The culture process includes a culture process for seeds and pilot-scale diatom strains and a culture process for large-scale diatom strains; The seed and pilot-scale diatom strain cultivation process is as follows: a single marine strain is cultured in F / 2+Si medium for activation, and a single freshwater strain is cultured in CSI medium for activation, and then incubated in a photobioreactor under the following incubation conditions: light intensity of 2000-2500 lux, light incubator temperature of 25±2°C, and light / dark cycle ratio of 14:10 hours; The large-scale cultivation process of diatom strains is as follows: the diatom strains are added to the F / 2+Si culture medium according to the weight ratio to form symbiotic diatoms, the culture is activated, and then incubated in a photobioreactor. The incubation conditions are as follows: light intensity of 3000-6000 lux, light incubator temperature of 25±2ºC, continuous lighting time of not less than 3 days, air is injected, and the pH is controlled at 7.0±2. At the end of the culture, the air supply is stopped; S2. The harvesting process is as follows: After the incubation, the diatom strain was settled to solid-liquid separation, the supernatant was removed, and the precipitated diatom material was collected; S3, freeze drying process: Mixing the diatom material collected in step S2 with 0.1% by weight of cactus mucus and filtered black soil to prepare a pre-diatom product, and freeze-drying the pre-diatom product to obtain a freeze-dried pre-diatom product; S4. Final product processing: The revival rate, moisture content, and heavy metal content of the freeze-dried pre-diatom product obtained in step S3 are analyzed to prepare a composite diatom powder live food water purifier for purifying water quality.

8. The method for preparing a composite diatom powder live food water purifier for purifying water according to claim 7, characterized in that: The F / 2+Si medium used in the cultivation process of large-scale diatom strains includes the following components by weight concentration: ammonium sulfate is 100 mg / L, urea is 75 mg / L, NPK is 15 mg / L, sterilized NaNO3 is 15 mg / L, (NH4)(H2PO4) is 15 mg / L, sterilized Na2HPO4·2H2O is 0.0057g / L, Na2SiO3.9H2O is 0.03g / L, vitamins are 1ml / L, vitamin B 12 is 0.0005g / L, vitamin B6 is 0.1g / L, biotin is 0.0005g / L, Na2EDTA·2H2O is 4.36g / L, FeCl3·6H2O is 3.15g / L, MnCl2·4H2O is 0.18g / L, ZnSo4.7H2O is 0.022g / L, CoCl2·6H2O is 0.01g / L, and Na2MoO4·2H2O is 0.006g / L.

9. The method for preparing a composite diatom powder live food water purifier for purifying water according to claim 7, characterized in that: The freeze-drying process in step S3 includes, in order, an automatic freeze-drying process and a manual freeze-drying process; The freeze-drying automatic processing parameters are as follows: the computer program is set to freeze-drying automatic mode and freeze-drying is started; the freeze-drying parameters are as follows: starting temperature is -40°C, pressure is 0.01 MPa, duration is 30 minutes, and sublimation freeze-drying is performed at a temperature increase of 5°C every 30 minutes until the temperature reaches 20°C; The freeze-drying manual processing parameters are as follows: when the temperature rises to 20°C, adjust the computer program to freeze-drying manual, turn on the air dehumidifier, set the temperature to 25°C and the humidity to 30%, and wait until the freeze-drying box has no negative pressure, and prepare to receive the material.

10. A method for resuscitating a composite diatom powder live food water purifier for purifying water prepared according to any one of claims 1 to 9, characterized in that: The resuscitation method comprises the following steps: The composite diatom powder live food water purifier can be put into a water body or a culture medium to realize the resuscitation of the composite diatom powder live food water purifier.

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