A hair care and growth composition and its preparation method and application

Abstract

The present invention discloses a hair care and growth composition, and a preparation method and application thereof. The hair care and growth composition is made of the following components in percentage by mass: 10-20% of lactobacillus / turmeric root extract fermentation lysate filtrate, 5-10% of rhamnosilanol, 1-5% of Torreya grandis oil, 1-3% of surfactant, and the balance of deionized water. The hair care and growth composition of the present invention can provide sufficient nutrition for hair follicle cells, activate various metabolic activities in hair follicles through various pathways, promote the regrowth of hair follicles that have entered a dormant period, grow new hair, increase the thickness, volume and strength of hair, and make hair healthier and denser. The three active ingredients play a synergistic role in hair care and growth, and are suitable for people with hair loss caused by various reasons, and have high safety and comfort in use.

Description

Technical Field

[0001] The invention belongs to the technical field of hair care agents, and in particular relates to a hair care and growth composition and a preparation method and application thereof. Background Art

[0002] Hair loss refers to the phenomenon of hair loss. Normally lost hair is in the catagen and telogen phases. Since the hair entering the catagen and the hair entering the new growth phase are constantly in a dynamic balance, the normal amount of hair can be maintained. Pathological hair loss refers to abnormal or excessive hair loss. With the increasing social pressure and the accelerated pace of life, the continuous deterioration of the environment, and the poor eating habits, our unhealthy and sub-healthy conditions are also increasing day by day, and the number of hair loss patients in China is increasing.

[0003] Relevant studies have shown that hair loss is mainly caused by the degeneration and atrophy of hair follicle cells in the scalp, which leads to insufficient nutrition for the hair. Therefore, to effectively solve the problem of hair loss, providing sufficient nutrition for hair follicle cells is the key; at the same time, with appropriate hair follicle growth factors, it can not only effectively prevent hair loss, but also promote the regrowth of dormant hair follicles and grow new hair, thus completely solving the problem of hair loss. At present, there are many products on the market that claim to prevent hair loss and promote hair growth. However, the effects of hair loss prevention and hair growth are not very ideal, and the scope of applicable population is narrow, and the effects of use by different populations are obviously different. Therefore, it is of great practical significance and broad market application prospects to develop a hair care and growth product that is quick in preventing hair loss and promoting hair growth, suitable for most people at this stage, and has high safety and comfort. Summary of the invention

[0004] The first object of the present invention is to provide a hair care and growth composition, which can penetrate into the scalp to nourish hair follicles, promote hair growth, and also nourish hair, repair hair strands, etc., so as to make hair healthier. Specifically, the hair care and growth composition is made of the following components in percentage by mass:

[0005]

[0006] The lactobacillus / turmeric root extract fermentation lysate filtrate of the present invention is prepared by the following steps:

[0007] S1, mixing turmeric root with distilled water and crushing it to obtain a slurry, then adding cellulase and amylase to hydrolyze it, centrifuging it, and collecting the supernatant; extracting the precipitate with ethanol aqueous solution, filtering it, and obtaining an extract;

[0008] S2, mixing the supernatant of S1 with glucose, adjusting the pH, and sterilizing to obtain a composite culture medium;

[0009] S3, inoculating lactobacillus into a composite culture medium for fermentation to obtain a fermentation culture solution; crushing the fermentation culture solution with a cell crusher, centrifuging, and taking the supernatant; filtering the supernatant with a microporous filter membrane to obtain a filtrate; mixing the filtrate with the extract of S1 to obtain a lactobacillus / turmeric root extract fermentation lysate filtrate.

[0010] Preferably, in step S1, the amount of cellulase and amylase added is 1-2wt% of the slurry; the weight ratio of cellulase to amylase is 1:1; the hydrolysis conditions are 45-55°C, 15-30min; the ethanol-water solution is mixed in a volume ratio of ethanol:water = 75:25, and the extraction time is 1-2h.

[0011] Preferably, in step S2, the supernatant and glucose are mixed in a ratio of 3-5 g / 100 mL; and the pH is adjusted to 6-7.

[0012] Preferably, in step S3, the fermentation condition is anaerobic fermentation at 35-45° C. for 24-48 hours.

[0013] Preferably, the surfactant is alkyl glucoside or hydrogenated castor oil.

[0014] The second object of the present invention is to provide a method for preparing the above-mentioned hair care and growth composition, comprising the following steps: adding a surfactant to deionized water, stirring to dissolve, then sequentially adding a lactobacillus / turmeric root extract fermentation lysate filtrate, rhamnosyl silanol, and Torreya grandis oil, and continuing to stir and mix to obtain the hair care and growth composition.

[0015] The third object of the present invention is to provide the use of the above-mentioned hair care and growth composition in the preparation of products with hair care and hair growth effects.

[0016] Preferably, the product comprises a cosmetic.

[0017] Preferably, the amount of the hair care and growth composition added to the cosmetics is 2-10 wt%.

[0018] The hair care and growth composition provided by the present invention is a lactobacillus / turmeric root extract fermentation lysate filtrate, rhamnosilanol, and Torreya grandis oil as active ingredients, and the four are used in combination to exert significant hair care and hair growth effects. Among them, the lactobacillus / turmeric root extract fermentation lysate filtrate, compared with the ordinary lactobacillus fermentation lysate, contains ingredients such as curcumin, has multiple effects such as antioxidant, anti-inflammatory, antibacterial, blood circulation promotion, and oil secretion inhibition, and promotes hair growth and prevents hair loss by activating the vitamin D receptor gene in the hair follicles and inhibiting 5α-reductase; it contains bacteria and all active ingredients synthesized during the fermentation process, which can nourish hair follicle cells, promote hair follicles to revitalize, improve blood circulation, help nourish and oxygenate the roots, increase the thickness and strength of hair, and make hair healthier and thicker. Therefore, compared with lactobacillus fermentation lysate, the lactobacillus / turmeric root extract fermentation lysate filtrate has more outstanding effects in hair care and hair growth. The present invention uses turmeric root enzymatic hydrolysis supernatant as culture medium to perform anaerobic fermentation on lactobacillus to obtain lactobacillus / turmeric root extract fermentation lysate filtrate, which can significantly enrich nutrients that nourish the growth of hair follicles, help activate various metabolic activities in the hair follicles, and reduce irritation.

[0019] Rhamnose silanol (SIRHAMNOSE) is a combination of rhamnose and silicone, which has good dermal affinity, making rhamnose have better permeability, bioavailability and activity. Rhamnose can increase the expression of laminin, type IV collagen, type VII collagen and basement membrane proteoglycan, stimulate fibroblasts, provide elasticity for hair fibers, strengthen hair roots, and make hair healthier. The silicon element in silanol can stimulate the synthesis of collagen and elastin, promote hair growth and reduce fragility and hair loss.

[0020] Torreya oil has antioxidant, bactericidal and anti-inflammatory effects. It promotes the strength of hair and improves hair loss by scavenging free radicals, promoting cell metabolism and maintaining homeostasis of the intracellular environment. The plant fatty acids it contains can penetrate into the surface of the scalp, improve the quality of the scalp by acting on the sebum layer, and maintain its barrier function. It is rich in vitamins, fatty acids and other nutrients, and can be used as a regulator to slow down the natural aging process, improve the hair growth environment, and promote hair growth.

[0021] The present invention can provide sufficient nutrition for hair follicle cells by compounding the above three active ingredients, activate various metabolic activities in hair follicles through various pathways, promote the regrowth of hair follicles that have entered a dormant period, grow new hair, increase the thickness, volume and strength of hair, and make hair healthier and thicker. The three active ingredients play a synergistic role in hair care and growth, and are suitable for people with hair loss caused by various reasons, and have high safety and comfort in use. DETAILED DESCRIPTION

[0022] The following examples are provided to further illustrate the present invention, but are not intended to limit the present invention. The components of the formulations in the following examples are all conventional commercially available products unless otherwise specified.

[0023] Example 1

[0024] The preparation of the lactobacillus / turmeric root extract fermentation lysate filtrate comprises the following steps:

[0025] S1. Wash the turmeric root and mix it with distilled water, then crush it to obtain a slurry, then add 1% of the slurry weight of mixed enzyme (cellulase: amylase = 1:1, weight ratio) at 50°C for 30 minutes, centrifuge, and collect the supernatant; extract the precipitate with ethanol aqueous solution (ethanol: water = 75:25, volume ratio) at room temperature for 60 minutes, filter, and obtain an extract;

[0026] S2, mixing the supernatant of S1 with glucose at a ratio of 4 g / 100 mL, adjusting the pH to 6, and sterilizing to obtain a composite culture medium;

[0027] S3. Inoculate lactobacillus into the composite culture medium and perform anaerobically fermentation at 45°C for 48 hours to obtain a fermentation culture medium; crush the fermentation culture medium with a cell crusher, centrifuge and take the supernatant; filter the supernatant with a 0.45 μm microporous filter membrane to obtain a filtrate; mix the filtrate with the extract of S1 at a volume ratio of 5:1, and add the lactobacillus / turmeric root extract fermentation lysate filtrate.

[0028] The lactobacillus / turmeric root extract fermentation lysate filtrate prepared in Example 1 was used to prepare the hair care and growth compositions of the following examples.

[0029] Example 2

[0030] A hair care and growth composition is prepared from the following components in percentage by mass: 18% of lactobacillus / turmeric root extract fermentation lysate filtrate, 7% of rhamnosyl silanol, 4% of torreya oil, 2% of octyl / decyl glucoside, and the balance of deionized water.

[0031] Preparation steps: add octyl / decyl glucoside to deionized water, stir to dissolve, then add lactobacillus / turmeric root extract fermentation lysate filtrate, rhamnosyl silanol, and Torreya grandis oil in sequence, continue to stir and mix, and obtain a hair care and growth composition.

[0032] Example 3

[0033] A hair care and growth composition is prepared from the following components in percentage by mass: 10% of lactobacillus / turmeric root extract fermentation lysate filtrate, 10% of rhamnosyl silanol, 5% of torreya oil, 3% of octyl / decyl glucoside, and the balance of deionized water.

[0034] The preparation steps are as in Example 2.

[0035] Example 4

[0036] A hair care and growth composition is prepared from the following components in percentage by mass: 15% of lactobacillus / turmeric root extract fermentation lysate filtrate, 8% of rhamnosyl silanol, 3% of torreya oil, 1% of octyl / decyl glucoside, and the balance of deionized water.

[0037] The preparation steps are as in Example 2.

[0038] Example 5

[0039] A hair care and growth composition is prepared from the following components in percentage by mass: 20% of lactobacillus / turmeric root extract fermentation lysate filtrate, 5% of rhamnosyl silanol, 2% of torreya oil, 1% of hydrogenated castor oil, and the balance of deionized water.

[0040] The preparation steps are as in Example 2.

[0041] Example 6

[0042] A hair care and growth composition is prepared from the following components in percentage by mass: 16% of lactobacillus / turmeric root extract fermentation lysate filtrate, 6% of rhamnosylsilanol, 1% of torreya oil, 2% of hydrogenated castor oil, and the balance of deionized water.

[0043] The preparation steps are as in Example 2.

[0044] Comparative Example 1

[0045] The preparation of a hair care and growth composition is basically the same as that of Example 2, except that it does not contain Torreya grandis oil.

[0046] Comparative Example 2

[0047] The preparation of a hair care and growth composition is substantially the same as that of Example 2, except that it does not contain rhamnose silanol.

[0048] Comparative Example 3

[0049] The preparation of a hair care and growth composition is substantially the same as that of Example 2, except that the composition does not contain the lactobacillus / turmeric root extract fermentation lysate filtrate.

[0050] Comparative Example 4

[0051] The preparation of a hair care and growth composition is substantially the same as that of Example 2, except that it does not contain rhamnosylsilanol and Torreya grandis oil.

[0052] Comparative Example 5

[0053] The preparation of a hair care and growth composition is substantially the same as that of Example 2, except that the composition does not contain the lactobacillus / turmeric root extract fermentation lysate filtrate and Torreya grandis oil.

[0054] Comparative Example 6

[0055] The preparation of a hair care and growth composition is substantially the same as that of Example 2, except that the composition does not contain the lactobacillus / turmeric root extract fermentation lysate filtrate and rhamnosylsilanol.

[0056] Comparative Example 7

[0057] A hair care and growth composition is prepared from the following components in percentage by mass: 18% of lactobacillus fermentation lysate, 7% of rhamnosyl silanol, 4% of torreya oil, 2% of octyl / decyl glucoside, and the balance of deionized water.

[0058] 1. Safety evaluation

[0059] The hair care and growth compositions prepared in Examples 2-6 of the present invention were tested with reference to the relevant requirements and determination methods of the "Human Skin Patch Test" in the 2015 edition of the "Hygiene Standards for Cosmetics" issued by the Ministry of Health, and the skin reactions were observed at 0h, 6h, 12h, 24h, and 48h, respectively. The experimental results of all volunteer subjects were negative, and no adverse skin reactions occurred. This indicates that the hair care and growth compositions provided by the present invention will not cause allergic reactions, have low irritation, and have no side effects.

[0060] 2. Performance Test

[0061] Healthy female volunteers who are troubled by excessive hair loss and mild hair thinning are selected. The inclusion criteria of the subjects are: 1) healthy women aged 18-60 years old, with hair length between 10-40 cm; 2) troubled by excessive hair loss and mild hair thinning, and the hair loss count according to the 60-combing method is greater than 10 hairs, and still greater than 10 hairs after a 2-week washing period; 3) no special hairdressing treatments such as hair dyeing, perming, and styling have been performed in the past month. The subjects are randomly divided into 12 groups, 7 people in each group, and the hair care and growth compositions prepared in Examples 2-6 and Comparative Examples 1-7 are used respectively. The method of use is: after the hair is wet, take an appropriate amount of the hair care and growth composition and apply it evenly on the hair and scalp, gently massage for 10 minutes, use it once every 1 day, and use it continuously for 2 months.

[0062] Each group of subjects was evaluated for hair base values ​​before using the product, including hair loss count, hair follicle density, and hair root toughness. The same evaluation and test were performed again after 2 months of product use. Among them, 1) Hair loss count: 60 times combing method was used to count the number of hairs lost in 60 combings; 2) Hair follicle density: a test area of ​​2cm x 2cm was selected at the hair loss area on the subject's head, and positioning marks were made. The hair follicle analyzer was used to measure the changes in hair follicle density in the test area; 3) The hair root toughness test method was: randomly select 1 hair from the forehead area, left temple area, and occipital area of ​​the subject, and tie the pull ring of the tensioner about 2cm away from the hair root to test the pulling force required to pull out each hair. All data were averaged. The results are shown in Table 1-2.

[0063] Table 1. Evaluation results of anti-hair loss and hair growth effects

[0064]

[0065] The results in Table 1 show that after using the hair care and growth composition of the present invention for 2 months, the amount of hair loss can be significantly reduced compared to before use, and the hair loss rate is reduced by about 50% to 60%, indicating that the hair care and growth composition of the present invention has the effect of preventing hair loss. In addition, compared to before use, after using the hair care and growth composition of the present invention for 2 months, the hair follicle density increased by about 40% to 50%, indicating that the hair care and growth composition of the present invention has a hair growth effect. As can be seen from Comparative Examples 1-6, the lactobacillus / turmeric root extract fermentation lysate filtrate, rhamnosyl silanol, and Torreya grandis oil are used as active ingredients, and the three are used in combination to produce a synergistic effect of preventing hair loss and growing hair, which is better than the single use of the above components or the combined use of two or more. As can be seen from Comparative Example 7, compared with the lactobacillus fermentation lysate, the lactobacillus / turmeric root extract fermentation lysate filtrate has a more outstanding effect in preventing hair loss and growing hair, and has better synergy with rhamnosyl silanol and Torreya grandis oil.

[0066] Table 2. Evaluation results of enhancing hair root strength

[0067] Group Pull force before use N Tension force after use N Improvement rate% Example 2 0.72 1.11 54.2 Example 3 0.65 0.96 47.7 Example 4 0.68 1.02 50.0 Example 5 0.76 1.16 52.6 Example 6 0.80 1.17 46.2 Comparative Example 1 0.74 1.02 37.8 Comparative Example 2 0.62 0.84 35.5 Comparative Example 3 0.83 1.09 31.3 Comparative Example 4 0.70 0.84 20.0 Comparative Example 5 0.82 0.95 15.9 Comparative Example 6 0.67 0.76 13.4 Comparative Example 7 0.78 1.13 44.9

[0068] The results in Table 2 show that after using the hair care and growth composition of the present invention for 2 months, the pulling force of the hair being pulled out of the hair follicles increased by about 45-55% compared to before use, indicating that the hair care and growth composition of the present invention can improve the adhesion of the scalp to the hair follicles, make the hair roots strong, and have a good effect of strengthening the hair roots. As can be seen from Comparative Examples 1-6, the lactobacillus / turmeric root extract fermentation lysate filtrate, rhamnosyl silanol, and Torreya grandis oil are used as active ingredients, and the three are used in combination to produce a synergistic effect of strengthening the hair roots, which is better than the effect of using the above components alone or in combination of two or more. As can be seen from Comparative Example 7, compared with the lactobacillus fermentation lysate, the lactobacillus / turmeric root extract fermentation lysate filtrate has a more outstanding effect in strengthening the hair roots, and has better synergy with rhamnosyl silanol and Torreya grandis oil.

[0069] The above are only preferred embodiments of the present invention. It should be noted that the above preferred embodiments should not be regarded as limiting the present invention, and the protection scope of the present invention should be based on the scope defined by the claims. For ordinary technicians in this technical field, several improvements and modifications can be made without departing from the spirit and scope of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A hair care and growth composition, characterized in that: Made of the following components in percentage by mass: Lactobacillus / Turmeric root extract fermentation lysate filtrate 10~20% Rhamnosylsilanol 5~10% Torreya grandis oil 1~5% Surfactant 1~3% Deionized water balance; The lactobacillus / turmeric root extract fermentation lysate filtrate is prepared by the following steps: S1. Mix turmeric root with distilled water and crush it to obtain a slurry, then add cellulase and amylase to hydrolyze at 45-55°C for 15-30 min, the amount of cellulase and amylase added is 1-2wt% of the slurry, the weight ratio of cellulase to amylase is 1:1, centrifuge, and collect the supernatant; The precipitate is extracted with an ethanol aqueous solution and filtered to obtain an extract; S2, mix the supernatant of S1 with glucose at a ratio of 3-5 g / 100 mL, adjust the pH to 6-7, sterilize, and obtain a composite culture medium; S3. Inoculate lactobacillus into the composite culture medium and perform anaerobically fermentation at 35-45°C for 24-48 hours to obtain a fermentation culture solution; crush the fermentation culture solution with a cell crusher, centrifuge and take the supernatant; filter the supernatant with a microporous filter membrane to obtain a filtrate; mix the filtrate with the extract of S1 to obtain a lactobacillus / turmeric root extract fermentation lysate filtrate.

2. The hair care and growth composition according to claim 1, characterized in that: The surfactant is alkyl glucoside or hydrogenated castor oil.

3. A method for preparing the hair care and growth composition according to claim 1 or 2, characterized in that: The method comprises the following steps: adding a surfactant into deionized water, stirring and dissolving, then sequentially adding lactobacillus / turmeric root extract fermentation lysate filtrate, rhamnosyl silanol and torreya grandis oil, and continuing to stir and mix to obtain a hair care and growth composition.

4. Use of the hair care and growth composition according to claim 1 or 2 in preparing products having hair care and hair growth effects.

5. The use according to claim 4, characterized in that: The products include cosmetics.

6. The use according to claim 5, characterized in that: The amount of the hair care and growth composition added to the cosmetics is 2-10 wt%.

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