Composition with skin brightening effect and application thereof

Abstract

The invention belongs to the technical field of skin care compositions, discloses a composition with skin complexion brightening effect and application thereof, the composition with skin complexion brightening effect, according to weight percentage, comprises the following components: acerola cherry extract 85-90%, ergothioneine 5-10%, carnosine 3-5%, lactobacillus fermentation lysate 2-5%; the composition with skin complexion brightening effect is applied to skin care products, and the content of the composition with skin complexion brightening effect in the skin care products is ≥1wt%. The present invention adopts acerola cherry extract, ergothioneine, carnosine and lactobacillus fermentation lysate compound, synergistically improves the skin's antioxidant capacity while protecting and promoting the synthesis and promotion of protein, effectively improves the skin complexion glossiness, and makes the skin more transparent and bright.

Description

Technical Field

[0001] The invention relates to the technical field of skin care compositions, and in particular to a composition having the effect of brightening skin complexion and an application thereof. Background Art

[0002] Skin is the largest multifunctional organ in the human body. It is affected by internal genetic factors and external environmental factors such as light, air pollution, and lifestyle, and faces skin conditions such as dull skin, darker skin, uneven skin tone, pigmentation, and precipitated spots. As people continue to pursue beauty and demand healthy skin color, the demand for skin care products that brighten the skin is increasing.

[0003] The skin color of a person is mainly determined by the content of melanin in the skin, the thickness of the epidermis and the scattering of optical fibers on the skin surface, that is, the glossiness of the skin. The function of brightening skin care products is not only to reduce the melanin in the skin, but more importantly to enhance the glossiness of the skin.

[0004] The existing brightening ingredients on the market are mainly synthetic chemical ingredients such as VC and its derivatives, kojic acid and its derivatives, arbutin, hydroquinone, etc., such as D1: Chinese patent application 202110412766.0 discloses a PHAR H Obsidian VC essence and its production process, consisting of group A water, disodium EDTA, group B composed of the following mass fractions: polyacrylate cross-linked polymer-6, sclerotium gum, sodium hyaluronate, diglycerin; group C composed of the following mass fractions: BlueCare ATO, glycerin; group D composed of the following mass fractions: VC fine powder, butylresorcinol, water; group E composed of the following mass fractions: water, anhydrous sodium sulfite; group F composed of the following mass fractions: water, dipotassium glycyrrhizinate; group G composed of the following mass fractions: amino acid moisturizer, yeast fermentation lysate filtrate, polyglutamic acid, gentian anti-irritation factor and E1003.

[0005] D1 uses VC, a commonly used brightening ingredient on the market, as the main ingredient of the essence, which can remove oxygen free radicals, enhance the skin's antioxidant capacity, reduce photoaging, and improve uneven skin tone caused by pigmentation, making the skin have better gloss; however, synthetic chemical ingredients such as VC and its derivatives, kojic acid and its derivatives, arbutin, hydroquinone, etc. may damage the epidermal barrier by exfoliating the skin, which is irritating, or act as strong tyrosinase inhibitors to damage or even kill melanocytes, thereby causing melanin imbalance. In addition, these ingredients reduce local melanin pigmentation in the skin by participating in the skin melanin pathway, but they cannot solve the problem of overall uneven skin tone. Once used, there is a risk of skin darkening again.

[0006] Based on this, D2: Chinese patent 202110813673.9 discloses a composition and a cosmetic composition with the effect of whitening and brightening skin color. The composition with the effect of whitening and brightening skin color is composed of the following components by weight: citrus peel extract: 0.1-5 parts; Cynanchum erythrorhizon fruit extract: 0.1-10 parts; niacinamide: 0.1-10 parts; D-panthenol: 0.1-5 parts; soluble proteoglycan: 0.1-3 parts.

[0007] The above patent uses a specific proportion of citrus peel extract, ophiopogon japonicus fruit extract, niacinamide, D-panthenol and soluble proteoglycan in combination, which can overcome the shortcomings of traditional whitening agents and achieve all-round whitening through safe, mild, effective and synergistic effects, thereby achieving the purpose of safe skin care and effective whitening. It can be used for daily care of people with all skin types.

[0008] The above patent uses citrus peel extract, ophiopogon japonicus fruit extract and other natural plant extracts as the main ingredients to achieve safe, gentle and effective skin whitening technology, which provides certain inspiration for the direction of technical research and development. However, in the existing technology, there are not many skin-lightening compositions based on natural plant extracts, which cannot meet the needs of different user groups. Therefore, it is the current market development demand to develop more compositions with skin-lightening effects based on different natural plant extracts. Summary of the invention

[0009] One of the purposes of the present invention is to provide a composition with the effect of brightening skin complexion, so as to solve the technical problem that there are few skin-lightening compositions based on natural plant extracts in the prior art and more skin-lightening compositions need to be further developed, and to develop a composition that synergistically improves the brightness of skin complexion and improves the skin's antioxidant capacity.

[0010] Another object of the present invention is to provide an application of a composition with skin brightening effect. The application of the composition with skin brightening effect in skin care products can effectively improve the glossiness of the skin, and tighten the skin, making the skin more transparent and bright.

[0011] To achieve the above object, the present invention provides a composition with skin brightening effect, which comprises the following components by weight percentage: 85-90% acerola cherry extract, 5-10% ergothioneine, 3-5% carnosine, and 2-5% lactobacillus fermentation lysate.

[0012] Preferably, the composition comprises the following components by weight percentage: 85% acerola cherry extract, 5-10% ergothioneine, 3-5% carnosine, and 2-5% lactobacillus fermentation lysate.

[0013] Further preferably, the composition comprises the following components: 85% acerola cherry extract, 7-10% ergothioneine, 3-5% carnosine, and 2-5% lactobacillus fermentation lysate.

[0014] The present invention also discloses an application of a composition having skin-lightening effect. The composition having skin-lightening effect is applied in skin care products. The content of the composition having skin-lightening effect in the skin care products is ≥1wt%.

[0015] Preferably, the content of the composition having skin lightening effect in the skin care product is 1-2 wt %.

[0016] Furthermore, the skin care products include lotions, creams, sprays, essences, essential oils, cleansers, lotions, facial masks, and gels.

[0017] Beneficial Effects

[0018] Compared with the prior art, the present invention has at least the following advantages:

[0019] (1) The present invention adopts acerola cherry extract, ergothioneine, carnosine and lactobacillus fermentation lysate to synergistically improve the antioxidant capacity of the skin while protecting and promoting the synthesis and improvement of protein, effectively improving the skin's complexion glossiness and making the skin more transparent and bright;

[0020] (2) The present invention mainly uses acerola cherry extract, a natural plant extract, supplemented with ergothioneine, carnosine and lactobacillus fermentation lysate and other components, which has a synergistic skin brightening effect while maintaining a high degree of mildness, thereby achieving a safe skin care brightening effect;

[0021] (3) The present invention discloses the use of a composition with skin lightening effect. When the composition with skin lightening effect is used in skin care products, when its content is ≥1, it has a significant skin lightening effect and mild performance. DETAILED DESCRIPTION

[0022] The present invention will be further described below in conjunction with the embodiments, but it does not constitute any limitation to the present invention. Any limited number of modifications made within the scope of the claims of the present invention are still within the scope of the claims of the present invention.

[0023] In order to explain the technical content of the present invention in detail, further description is given below in conjunction with the implementation modes.

[0024] In the following examples and comparative examples, acerola cherry extract was purchased from Guangzhou Jianeng Biotechnology Co., Ltd., ergothioneine was purchased from Huaxi Biotechnology Co., Ltd., carnosine was purchased from Shenzhen Redlin Biotechnology Co., Ltd., lactobacillus fermentation lysate was purchased from Bestmei Biotechnology (Zhejiang) Co., Ltd., citrus fruit extract was purchased from Guangzhou Jiaye Biotechnology Co., Ltd., ekdoin was purchased from Guangzhou Merck Biotechnology Co., Ltd., inositol was purchased from Guangzhou Hengtao Trading Co., Ltd., bifid yeast fermentation product lysate was purchased from Guangzhou Miaosen Biotechnology Co., Ltd., and micrococcus lysate was purchased from Zhejiang Moda Biotechnology Co., Ltd.

[0025] It should be noted that the technical solution of the present invention can be implemented through other commercially available products and is not limited to the extracts provided by the above-mentioned manufacturers.

[0026] In the following examples and comparative examples, unless otherwise specified, the parts are parts by weight and the % are percentages by weight.

[0027] Example 1

[0028] A composition with skin brightening effect is obtained by uniformly mixing 90 parts of acerola cherry extract, 5 parts of ergothioneine, 3 parts of carnosine and 2 parts of lactobacillus fermentation lysate.

[0029] Example 2

[0030] A composition with skin brightening effect is obtained by uniformly mixing 85 parts of acerola cherry extract, 10 parts of ergothioneine, 3 parts of carnosine and 2 parts of lactobacillus fermentation lysate.

[0031] Example 3

[0032] A composition with skin brightening effect is obtained by uniformly mixing 85 parts of acerola cherry extract, 5 parts of ergothioneine, 5 parts of carnosine and 5 parts of lactobacillus fermentation lysate.

[0033] Example 4

[0034] A composition with skin brightening effect is obtained by uniformly mixing 85 parts of acerola cherry extract, 7 parts of ergothioneine, 4 parts of carnosine and 4 parts of lactobacillus fermentation lysate.

[0035] Example 5

[0036] A composition with skin brightening effect is obtained by uniformly mixing 88 parts of acerola cherry extract, 7 parts of ergothioneine, 3 parts of carnosine and 2 parts of lactobacillus fermentation lysate.

[0037] Comparative Example 1

[0038] A composition with skin brightening effect is obtained by uniformly mixing 33.3 parts of ergothioneine, 33.3 parts of carnosine and 33.3 parts of lactobacillus fermentation lysate.

[0039] Comparative Example 2

[0040] A composition with skin brightening effect is obtained by uniformly mixing 89.5 parts of acerola cherry extract, 5.25 parts of carnosine and 5.25 parts of lactobacillus fermentation lysate.

[0041] Comparative Example 3

[0042] A composition with skin brightening effect is obtained by uniformly mixing 89.5 parts of acerola cherry extract, 5.25 parts of ergothioneine and 5.25 parts of lactobacillus fermentation lysate.

[0043] Comparative Example 4

[0044] A composition with skin brightening effect is obtained by uniformly mixing 89.5 parts of acerola cherry extract, 5.25 parts of ergothioneine and 5.25 parts of carnosine.

[0045] Comparative Example 5

[0046] A composition is obtained by uniformly mixing 85 parts of citrus fruit extract, 7 parts of ergothioneine, 4 parts of carnosine and 4 parts of lactobacillus fermentation lysate.

[0047] Comparative Example 6

[0048] A composition is obtained by uniformly mixing 85 parts of acerola cherry extract, 7 parts of ectoine, 4 parts of carnosine and 4 parts of lactobacillus fermentation lysate.

[0049] Comparative Example 7

[0050] A composition is obtained by uniformly mixing 85 parts of acerola cherry extract, 7 parts of ergothioneine, 4 parts of inositol and 4 parts of lactobacillus fermentation lysate.

[0051] Comparative Example 8

[0052] A composition is obtained by uniformly mixing 85 parts of acerola cherry extract, 7 parts of ergothioneine, 4 parts of carnosine and 4 parts of bifid yeast fermentation product lysate.

[0053] Comparative Example 9

[0054] A composition is obtained by uniformly mixing 85 parts of acerola cherry extract, 7 parts of ergothioneine, 4 parts of carnosine and 4 parts of micrococcal lysate.

[0055] Performance Testing

[0056] 1. Antioxidant performance test

[0057] 1.1 Determination of DPPH free radical scavenging ability:

[0058] Test method: add 3mL of phosphate buffer with pH value of 6.8, 4mL of 0.4mmol / LDPPH anhydrous ethanol solution, and 0.2mL of the sample to be tested to a 10mL centrifuge tube and mix thoroughly. After 30 minutes, centrifuge for 10 minutes (8000r / min), take the supernatant, measure the absorbance at 516nm, perform the operation three times in parallel and take the average value. The scavenging rate of the sample for DPPH free radicals = [1-(A1-A2) / A3]. Where A1 is the absorbance of the DPPH solution after adding the sample, A2 is the absorbance of the sample solution without adding DPPH (replaced with anhydrous ethanol), and A3 is the absorbance of the DPPH and aqueous solution without adding the sample. The higher the free radical scavenging rate of the sample to be tested, the stronger the antioxidant capacity.

[0059] 1.2 Determination of the ability to scavenge superoxide anion free radicals:

[0060] Test method: Prepare a buffer system (Tris-HCl-EDTA) with a pH value of 8.20. Add 0.6 mL of the sample to be tested to a 10 mL centrifuge tube, add 5.2 mL of buffer, use a pipette to add 0.2 mL of 0.045 mol / L pyrogallol solution, shake quickly, wait for the reaction for 5 minutes, and quickly measure the absorbance value at 325 nm in a dry cuvette, record it as A0, and measure it again after 10 minutes as A4. The absorbance values ​​measured by replacing the sample with distilled water are recorded as B0 and B4 respectively. Perform the operation in parallel for 3 times and take the average value. The inhibition rate of the sample on superoxide anions = [(B4-B0)-(A4-A0)] / (B4-B0). The higher the inhibition rate of the sample to be tested, the stronger the antioxidant capacity.

[0061] The free radical scavenging ability of Examples 1-5 and Comparative Examples 1-9 was tested according to the above test method, and the results are shown in Table 1;

[0062] Table 1 Antioxidant capacity test results of Examples 1-5 and Comparative Examples 1-9

[0063] Sample name DPPH free radical scavenging ability (%) Superoxide anion free radical scavenging ability (%) Example 1 96.7 93.6 Example 2 96.6 93.2 Example 3 96.2 94.5 Example 4 94.1 92.7 Example 5 95.3 93.7 Comparative Example 1 45.4 43.7 Comparative Example 2 60.2 58.7 Comparative Example 3 65.3 63.8 Comparative Example 4 63.8 61.4 Comparative Example 5 70.1 70.3 Comparative Example 6 68.2 66.7 Comparative Example 7 67.8 65.2 Comparative Example 8 74.5 72.8 Comparative Example 9 73.2 71.3

[0064] According to the results in Table 1, we can see that:

[0065] According to the comparison of the free radical scavenging ability data of Example 5 and Comparative Examples 1-4, the present invention adopts acerola cherry extract, ergothioneine, carnosine, and lactobacillus fermentation lysate to produce a synergistic effect of improving the skin's ability to scavenge DPPH free radicals and superoxide anion free radicals; wherein according to the data of Comparative Example 1, the influence of acerola cherry extract on free radical scavenging ability is relatively large, and in the case of losing acerola cherry extract, the skin's ability to scavenge DPPH free radicals and superoxide anion free radicals of the composition are greatly reduced; and according to the data of Comparative Examples 2-3, when the amount of acerola cherry extract is retained and increased, one of the components of ergothioneine, carnosine, and lactobacillus fermentation lysate is missing, and the ability to scavenge DPPH free radicals and superoxide anion free radicals that the composition can exert has significantly decreased to varying degrees; it is explained that the composition of the present invention is a synergistic effect of skin scavenging DPPH free radicals and superoxide anion free radicals obtained by compounding four components of acerola cherry extract, ergothioneine, carnosine, and lactobacillus fermentation lysate, and the four components synergize with each other and are indispensable.

[0066] According to the comparison of the free radical scavenging ability data of Example 4 and Comparative Examples 5-9, it can be seen that the effect of the technical solution of the present invention on synergistically improving the skin's ability to scavenge DPPH free radicals and superoxide anion free radicals is based on the compounding of four components: acerola cherry extract, ergothioneine, carnosine, and lactobacillus fermentation lysate; Comparative Example 5 uses citrus fruit extract with the same strong antioxidant effect to replace acerola cherry extract, Comparative Example 6 uses ectoine to replace ergothioneine, Comparative Example 7 uses inositol to replace carnosine, Comparative Examples 8 and Comparative Examples 9 respectively use fermentation lysates of other strains to replace lactobacillus fermentation lysate, all of which play a certain role in scavenging free radicals, but the overall effect is significantly weaker than that of Example 4, indicating that the present invention uses acerola cherry extract, ergothioneine, carnosine, and lactobacillus fermentation lysate to produce a synergistic effect of improving the skin's ability to scavenge DPPH free radicals and superoxide anion free radicals.

[0067] 2. Human skin gloss test

[0068] Test method: 33 subjects with dull, lack of luster, dry and lack of nourishment in the arm test area were selected. The test sample was applied to the inner arm for 14 consecutive days, and the gloss of the selected part of the inner arm skin was measured using the Glosssymeter GL200 skin gloss tester. The selected part was tested 3 times using the skin gloss probe and the average value was recorded. The larger the measured value, the higher the skin gloss.

[0069] Result determination: Before and after the trial of the product, the glossiness of the arm on the measured side was significantly improved compared with before the trial of the product (P < 0.05), indicating that the test substance has a brightening effect.

[0070] 2.1 Skin gloss test of essences with different contents of composition having skin brightening effect

[0071] Sample configuration:

[0072] According to the ratio in Table 2, an essence containing different amounts of the composition having skin brightening effect of Example 4 was prepared;

[0073] Table 2 Formula of essence with different contents

[0074]

[0075]

[0076] Samples 1-7 were tested according to the above-mentioned human skin glossiness test method, and the results are shown in Table 3;

[0077] Table 3 Skin gloss test results of essence containing different amounts of the composition with skin brightening effect of Example 4

[0078]

[0079] According to Table 3, we can see that:

[0080] Compared with sample 1 (negative control), samples 2-7 all showed a skin brightening effect; sample 2 had a certain brightening effect but no significance (P>0.05), samples 3-7 had a relatively obvious brightening effect (P<0.05), and the brightening effect increased with the increase of the content of the composition of Example 4; among them, the enhancement of the brightening effect of samples 6-7 tended to be gentle, and there was no obvious improvement compared with sample 5.

[0081] The above skin complexion glossiness experiment shows that the skin complexion brightening composition provided by the present invention is preferably used in an amount of 1-2wt% in skin care products. The product efficacy is not obvious if it is below 1wt%, and the product efficacy is not significantly improved if it is above 2wt%.

[0082] 2.2 Test on the glossiness improvement of human skin by essence containing 2 wt % of the composition of Examples 1-5 and Comparative Examples 1-9

[0083] According to the ratio of sample 5 in Table 2, an essence containing 2 wt % of the composition of Examples 1-5 and Comparative Examples 1-9 was prepared, and then tested according to the above-mentioned human skin glossiness test method. The results are shown in Table 4;

[0084] Table 4 Test results of the effect of 2 wt% of the essence of the compositions of Examples 1-5 and Comparative Examples 1-9 on the glossiness of human skin

[0085]

[0086]

[0087] According to the results in Table 4:

[0088] According to the comparison of the free radical scavenging ability data of Example 5 and Comparative Examples 1-4, the present invention adopts acerola cherry extract, ergothioneine, carnosine, and lactobacillus fermentation lysate to produce a synergistic effect of improving skin glossiness; wherein the data of Comparative Examples 1-4 also show that in the absence of any one of acerola cherry extract, ergothioneine, carnosine, and lactobacillus fermentation lysate in the technical scheme of the present invention, the other three substances can only perform a simple efficacy superposition, and in the simple efficacy superposition process, in the case where Comparative Example 1 and Comparative Example 2 are respectively missing acerola cherry and ergothioneine, the skin glossiness is improved. The skin glossiness improving effect is significantly decreased, indicating that acerola cherry and ergothioneine are better in improving the skin glossiness; however, in Comparative Examples 3 and 4, the skin glossiness improving effect shown by lacking one of carnosine and lactobacillus fermentation lysate when having acerola cherry and ergothioneine is also significantly decreased compared with Example 5, indicating that the composition of the present invention is a synergistic effect of improving skin glossiness obtained by compounding four components of acerola cherry extract, ergothioneine, carnosine, and lactobacillus fermentation lysate, and the four components synergize with each other and are indispensable.

[0089] According to the comparison of the free radical scavenging ability data of Example 4 and Comparative Examples 5-9, it can be seen that the synergistic improvement of the technical scheme of the present invention in improving skin gloss is based on the compounding of four components: acerola cherry extract, ergothioneine, carnosine, and lactobacillus fermentation lysate; Comparative Example 5 uses citrus fruit extract with the same strong antioxidant effect to replace acerola cherry extract, Comparative Example 6 uses ectoine to replace ergothioneine, Comparative Example 7 uses inositol to replace carnosine, Comparative Examples 8 and Comparative Examples 9 respectively use fermentation lysates of other strains to replace lactobacillus fermentation lysate, all of which have a certain effect of improving skin gloss. However, according to the data of Comparative Examples 5-9, it can be seen that the effect of improving skin gloss produced by the compounding of raw materials in Comparative Examples 5-9 is only a simple superposition of effects, and the various components do not produce a synergistic effect like the technical scheme of the present invention, and the improvement of skin gloss is significantly lower than the technical scheme of the present invention.

[0090] 3. Mildness test

[0091] Patch test

[0092] Test method: 33 volunteers aged 18-60 who meet the test requirements are selected as test subjects. Gently wipe the test area with a dry soft tissue, take 0.025g of the sample to be tested and add it to the patch tester. According to the preset sample position, stick the patch tester with the corresponding number on the corresponding test area. Quickly stick the patch tester from top to bottom to the corresponding position, gently press and smooth it, and apply it on the skin for 24 hours. After that, the tester will remove the patch tester and mark it with a skin marker. 0.5h after removing the patch (after the indentation disappears), record the results of naked eye observation.

[0093] Judgment criteria: Grading criteria for skin reactions in skin occlusive patch tests: Grade 0: negative reaction, no adverse reaction; Grade 1: suspicious reaction, only slight erythema; Grade 2: weak positive reaction (erythema reaction); erythema, infiltration, edema, and may have papules; Grade 3: strong positive reaction (herpes reaction); erythema, infiltration, edema, papules, and herpes; the reaction may extend beyond the test area; Grade 4: extremely strong positive reaction (confluent herpes reaction); obvious erythema, severe infiltration, edema, and confluent herpes; the reaction extends beyond the test area.

[0094] Examples 1-5 were subjected to skin patch tests according to the above test method, and the results are shown in Table 5;

[0095] Table 5 Mildness test results of the compositions of Examples 1-5

[0096]

[0097] According to Table 5, we can see that:

[0098] It can be seen that the compositions prepared by compounding cherry leaf extract, ergothioneine, carnosine and lactobacillus fermentation lysate of the present invention all showed adverse skin reactions, indicating that the compositions have good mildness.

[0099] The embodiments presented herein are only embodiments selected according to the combination of all possible embodiments. The appended claims should not be limited by the embodiments describing the present invention. Some numerical ranges used in the claims include sub-ranges therein, and the changes in these ranges should also be covered by the appended claims.

Claims

1. A composition having the effect of brightening skin complexion, characterized in that: The invention is prepared from the following components according to weight percentage: 85-90% of acerola cherry extract, 5-10% of ergothioneine, 3-5% of carnosine and 2-5% of lactobacillus fermentation lysate.

2. The composition having skin lightening effect according to claim 1, characterized in that: The invention is prepared from the following components according to weight percentage: 85% of acerola cherry extract, 5-10% of ergothioneine, 3-5% of carnosine and 2-5% of lactobacillus fermentation lysate.

3. The composition having skin lightening effect according to claim 1, characterized in that: The invention is prepared from the following components according to weight percentage: 85% of acerola cherry extract, 7-10% of ergothioneine, 3-5% of carnosine and 2-5% of lactobacillus fermentation lysate.

4. Use of the composition having skin lightening effect as claimed in any one of claims 1 to 3 in skin care products, characterized in that: The content of the composition with skin lightening effect in the skin care product is 1-2 wt %.

5. Use of the composition having skin lightening effect according to claim 4 in skin care products, characterized in that: The skin care products include lotions, creams, sprays, essences, essential oils, toners, facial masks and gels.

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