An enzyme-linked immunosorbent plate washer

Abstract

The application discloses a novel enzyme-linked immunosorbent assay plate washing device in the technical field of enzyme-linked immunosorbent assay plate washing, which comprises a plate washing box, a water distribution cylinder is fixedly connected to the bottom of the plate washing box, a water outlet plug is arranged at the bottom of the water distribution cylinder, a quantitative plug is arranged in the water distribution cylinder, a sliding cylinder is arranged between the quantitative plug and the water outlet plug, a bottom plug and a top plug are slidably connected to the outside of the sliding cylinder, a water discharge rod is slidably connected to the middle of the sliding cylinder, an upper ejector rod is fixedly connected to the bottom of the water discharge rod, a compression ring is elastically connected to the top of the quantitative plug, a plurality of compression rods are fixedly connected to the bottom of the compression ring, a quantitative rod is fixedly connected to the top of the compression ring, an upper lifting plate is fixedly connected to the top of the water discharge rod, a lower pressing plate is fixedly connected to the top of the quantitative rod, a connecting cylinder is fixedly connected to the top of the quantitative plug, a sealing plate is fixedly connected to the top of the connecting cylinder, the application realizes quantitative water injection and cleaning at the same time, avoids pollution, saves time, improves cleaning efficiency, greatly facilitates operation and simplifies control, has a simple overall structure and low cost.

Description

Technical Field

[0001] This invention relates to the field of enzyme-linked immunosorbent assay (ELISA) plate washing technology, specifically to an ELISA plate washer. Background Technology

[0002] Enzyme-linked immunosorbent assay (ELISA), one of many immunoassay techniques, is now widely used in clinical medicine, animal quarantine, food science, plant viruses, drug residues, and pest and disease control. This method boasts high specificity and sensitivity, capable of detecting almost all soluble antigen-antibody systems, with a minimum detection limit reaching ng or even pg. Compared to radioimmunoassay, ELISA offers advantages such as stable labeling and no radioactive hazard; compared to immunofluorescence assay, ELISA provides more objective result interpretation.

[0003] In enzyme-linked immunosorbent assay (ELISA), a complete ELISA kit should include a coated solid-phase carrier, enzyme conjugate, chromogenic substrate, reaction stop solution, various diluents and buffers, as well as standard solutions, calibration solutions, positive control or control serum, and negative control or control serum used in the assay. Since ELISA requires the use of multiple reagents, which are added sequentially according to the needs of the reaction, cleaning the ELISA plate, the reaction vessel, becomes an essential step. In ELISA experiments and assays, the ELISA plate is often used as the reaction vessel and enzyme carrier. During the experiment, the reaction reagents and the sample are added to each well, and the reaction is carried out in an environment simulating human body temperature. After the reaction, the unreacted reactants in the wells are washed away, leaving the reacted material bound to the well walls. This process is called plate washing. After washing, other reaction substrates can be added for the next experimental step.

[0004] Currently, the plate washing process in most laboratories is generally completed in two steps. The first step is to repeatedly inject washing solution into the microwells for cleaning; the second step is to remove the residual washing solution from the microwells. In the step of repeatedly injecting washing solution, laboratory technicians often use a squeeze bottle connected to a curved nozzle. Each time they squeeze the bottle, the water inside is squeezed out and injected into the microwell through the curved nozzle. Only one microwell can be injected at a time. This method of water injection is usually inefficient, and the amount of water injected cannot be controlled. Excessive water volume can also cause cross-contamination between microwells. Fully automated plate washing is relatively expensive and has limited applicability.

[0005] Based on this, the present invention designs an enzyme-linked immunosorbent assay (ELISA) plate washer to solve the above problems. Summary of the Invention

[0006] The purpose of this invention is to provide an enzyme-linked immunosorbent assay (ELISA) plate washer to solve the problems mentioned in the background art, such as the low efficiency of existing plate washing methods and water injection methods, the inability to control the water volume, the cross-contamination between micropores caused by excessive water volume, and the high cost of automatic plate washing.

[0007] To achieve the above objectives, the present invention provides the following technical solution:

[0008] An enzyme-linked immunosorbent assay (ELISA) plate washer includes a washing chamber with multiple flow guide nozzles connected to the bottom. Each flow guide nozzle has a water distribution cylinder fixedly connected to its top. The water distribution cylinder is fixedly connected to the bottom of the washing chamber. An outlet plug is located at the bottom of the water distribution cylinder. A metering plug is installed inside the water distribution cylinder. A through hole is formed between the metering plug and the outlet plug. A sliding cylinder is provided between the metering plug and the outlet plug. A bottom plug and a top plug are slidably connected outside the sliding cylinder. Both the top plug and the outlet plug are frustoconical, with the tip of the top plug facing upwards at the bottom of the metering plug, and the tip of the bottom plug facing downwards at the top of the outlet plug. A sealing spring is provided between the bottom plug and the top plug. A water discharge rod is slidably connected to the middle of the slide cylinder. An upper push rod is fixedly connected to the bottom of the water discharge rod. A pressure ring is elastically connected above the metering plug. Multiple pressure rods are fixedly connected to the bottom of the pressure ring. A metering rod is fixedly connected to the top of the pressure ring. An upper lifting plate is fixedly connected to the top of the water discharge rod. A lower pressure plate is fixedly connected to the top of the metering rod. A connecting cylinder is fixedly connected to the top of the metering plug. A sealing plate is fixedly connected to the top of the connecting cylinder. Water is stored between the sealing plate and the washing tank. A water inlet pipe is connected to the top of the washing tank.

[0009] Furthermore, the lower pressure plate is located below the upper lifting plate, and an operating shaft is rotatably connected inside the washing box. A cam is fixedly connected to the outer wall of the operating shaft, and the cam is located between the lower pressure plate and the upper lifting plate and is in contact with both.

[0010] Furthermore, a separation plate is fixedly connected inside the washing box, and a rotating gear is fixedly connected to the outer wall of the operating shaft. The sealing plate and the rotating gear are located on both sides of the separation plate. A sliding window is provided on the outer wall of the washing box, and a connecting plate is slidably connected inside the sliding window. A rack is fixedly connected to the inner end of the connecting plate, and a push plate is fixedly connected to the outer end. The rack is located below the rotating gear and meshes with it.

[0011] Furthermore, the width of the connecting plate is less than the length of the sliding window, and in its original state, the side of the connecting plate is close to one side of the sliding window. A floating plate is elastically connected inside the sliding window, and the connecting plate is slidably connected to the top of the floating plate.

[0012] Furthermore, a positioning ring is fixedly connected to the inner wall of the water distribution cylinder, the bottom plug is located below the positioning ring, the metering plug is slidably connected inside the water distribution cylinder, the sealing plate is slidably connected inside the washing tank, a through hole is opened at the top of the washing tank, an adjusting column is fixedly connected to the top of the sealing plate, the adjusting column passes through the through hole and slides with it, and the metering rod is a telescopic rod with one end fixedly connected to the top of the pressure ring and the other end fixedly connected to the bottom of the lower pressure plate.

[0013] Furthermore, the metering rod includes an outer cylinder and an inner rod. The outer cylinder is fixedly connected with multiple linkage rings. The inner rod is located inside the outer cylinder and its end is rotatably connected to a forked rod. A linkage rod is slidably connected to the middle of the inner rod. The bottom end of the linkage rod is located above the inner end of the forked rod. The top end of the linkage rod extends out of the top of the lower pressure plate and is fixedly connected to a docking plate. A synchronous spring is connected between the pressure ring and the sealing plate.

[0014] Furthermore, mounting grooves are opened on both sides of the bottom end of the inner rod, the forked rod is boomerang-shaped and rotatably connected in the mounting groove through a torsion spring shaft, a conical head is fixedly connected to the bottom of the linkage rod, a retaining spring is connected between the lower pressure plate and the sealing plate, a return spring is connected between the docking plate and the lower pressure plate, and the elastic force of the synchronization spring, retaining spring and return spring decreases in sequence.

[0015] Furthermore, a positioning leak plate and a sealing ring are fixedly connected inside the water inlet pipe. The positioning leak plate is located at the bottom of the sealing ring, and a sealing sheet is provided between the two. The diameter of the sealing sheet is larger than the inner diameter of the sealing ring and smaller than the inner diameter of the water inlet pipe.

[0016] Furthermore, the bottom of the washing tank is rotatably connected to multiple protective rods, and the bottom end of each protective rod is fixedly connected to a flat bottom plate. A water-absorbing pad is installed on the top of the flat bottom plate, and the flat bottom plate is used to block the guide nozzle.

[0017] Compared with the prior art, the beneficial effects of the present invention are:

[0018] This device is used by connecting an external water source via a flexible hose to the inlet pipe. Water enters the space between the sealing plate and the washing tank through the inlet pipe. First, press down the lower pressure plate; the pressure rod moves down, pressing down the top plug, thus opening the central hole of the metering plug. Since the space between the outlet plug and the metering plug is constant, the amount of water entering is also constant, thus achieving the function of metered water dispensing. After dispensing, release the lower pressure plate to return it to its original position, then lift the upper plate, causing the upper push rod to move upwards, pushing the bottom plug upwards, thereby opening the... The central through-hole of the water outlet plug allows water to flow out between the outlet plug and the metering plug, exiting through the bottom guide nozzle to simultaneously and quantitatively clean a row of holes on the plate. Because water is drawn and released quantitatively, there is no risk of overflow or contamination between holes due to poor water volume control, nor is there a problem of insufficient water resulting in poor cleaning. This ensures that water can be drawn and injected quantitatively and quickly into the holes in the same row, saving time, improving cleaning efficiency, greatly facilitating operation and simplifying control. The overall structure is simple and the cost is low. Attached Figure Description

[0019] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0020] Figure 1 This is a schematic diagram of the front view structure of the present invention;

[0021] Figure 2 This is a schematic diagram of the front half-section structure of the present invention;

[0022] Figure 3 This is an enlarged structural diagram of part A of the present invention;

[0023] Figure 4 This is a half-section schematic diagram of the water distribution cylinder from a side view of the present invention;

[0024] Figure 5 This is an enlarged structural diagram of part B of the present invention;

[0025] Figure 6 This is an enlarged structural diagram of part C of the present invention;

[0026] Figure 7 This is a schematic diagram of the rear view structure of the present invention;

[0027] Figure 8 This is a schematic diagram of the stepped cross-section structure from the rear view of the present invention;

[0028] Figure 9 This is a schematic diagram of the half-section structure of the rack from the rear view of the present invention.

[0029] The attached diagram lists the components represented by each number as follows:

[0030] 1. Washing tank; 2. Drain nozzle; 3. Water separator; 4. Outlet plug; 5. Metering plug; 6. Central through hole; 7. Slide cylinder; 8. Bottom plug; 9. Top plug; 10. Sealing spring; 11. Water discharge rod; 12. Top push rod; 13. Pressure ring; 14. Pressure rod; 15. Metering rod; 16. Upper lifting plate; 17. Lower pressure plate; 18. Connecting cylinder; 19. Sealing plate; 20. Water inlet pipe; 21. Operating shaft; 22. Cam; 23. Separating plate; 24. Rotating gear 25. Wheel; 26. Sliding window; 27. Connecting plate; 28. Rack; 29. ​​Push plate; 30. Floating plate; 31. Positioning ring; 32. Adjusting column; 33. Outer cylinder; 34. Inner rod; 35. Linkage ring; 36. Forked rod; 37. Linkage rod; 38. Connecting plate; 39. Synchronous spring; 40. Conical head; 41. Holding spring; 42. Return spring; 43. Positioning disc; 44. Sealing ring; 45. Sealing sheet; 46. Protective rod; 47. Flat bottom plate. Detailed Implementation

[0031] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0032] Please see Figure 1-9 The present invention provides a technical solution:

[0033] An enzyme-linked immunosorbent assay (ELISA) plate washer includes a washing chamber 1. Multiple flow nozzles 2 are connected to the bottom of the washing chamber 1. A water distribution cylinder 3 is fixedly connected to the top of each flow nozzle 2. The water distribution cylinder 3 is fixedly connected to the bottom of the washing chamber 1. An outlet plug 4 is located at the bottom of the water distribution cylinder 3. A metering plug 5 is installed inside the water distribution cylinder 3. A through hole 6 is formed between the metering plug 5 and the outlet plug 4. A sliding cylinder 7 is provided between the metering plug 5 and the outlet plug 4. A bottom plug 8 and a top plug 9 are slidably connected to the outside of the sliding cylinder 7. Both the top plug 9 and the bottom plug 8 are frustoconical, with the tip of the top plug 9 pointing upwards at the bottom of the metering plug 5, and the tip of the bottom plug 8 pointing downwards above the outlet plug 4. A sealing spring 10 is provided between the top plugs 9. A water discharge rod 11 is slidably connected to the middle of the slide cylinder 7. An upper push rod 12 is fixedly connected to the bottom of the water discharge rod 11. A pressure ring 13 is elastically connected above the metering plug 5. Multiple pressure rods 14 are fixedly connected to the bottom of the pressure ring 13. A metering rod 15 is fixedly connected to the top of the pressure ring 13. An upper lifting plate 16 is fixedly connected to the top of the water discharge rod 11. A lower pressure plate 17 is fixedly connected to the top of the metering rod 15. A connecting cylinder 18 is fixedly connected to the top of the metering plug 5. A sealing plate 19 is fixedly connected to the top of the connecting cylinder 18. The sealing plate 19 and the washing tank 1 are used to store water. A water inlet pipe 20 is connected to the top of the washing tank 1.

[0034] Please see Figure 1-3 When using this device, an external water source is connected to the inlet pipe 20 via a flexible hose. Specifically, a mineral water bottle with a water pump can be used as the water source. With the water pump turned on, water flows through the inlet pipe 20 into the space between the sealing plate 19 and the washing tank 1. At this time, the cylinder between the top of the metering stopper 5 and the connecting cylinder 18 is also filled with water. First, the lower pressure plate 17 is pressed down, causing the metering rod 15 to move downwards. This causes the pressure ring 13 and the pressure rod 14 to move downwards together. The downward movement of the pressure rod 14 presses down the top stopper 9, thereby opening the central through-hole 6 of the metering stopper 5. Water above the metering stopper 5 enters the space between the outlet stopper 4 and the metering stopper 5 through the central through-hole 6 until it is full. Since the space between the outlet stopper 4 and the metering stopper 5 is constant, water enters its interior. The water volume is also constant, thus achieving the function of quantitative water intake. After water intake is completed, the lower pressure plate 17 is released to return to its original position, and then the upper lifting plate 16 is lifted, which drives the water discharge rod 11 to lift up, thereby causing the upper push rod 12 to move up and push the bottom plug 8 upward, thereby opening the central through hole of the water outlet plug 4, allowing the water between the water outlet plug 4 and the quantitative plug 5 to flow out. It flows out through the bottom guide nozzle 2, thereby simultaneously quantitatively injecting water to clean a row of holes on the plate surface. Since the water is quantitatively taken out and then released, there will be no situation where the water volume is difficult to control, and there will be no overflow and interconnection between holes due to excessive water, nor will there be a situation where there is insufficient water and the cleaning effect is poor. This allows for quantitative and rapid water intake and injection of holes in the same row, saving time, improving cleaning efficiency, and greatly facilitating operation and simplifying control.

[0035] Please see Figure 2 ,8 -9, wherein the lower pressure plate 17 is located below the upper lifting plate 16, and an operating shaft 21 is rotatably connected inside the washing box 1. A cam 22 is fixedly connected to the outer wall of the operating shaft 21. The cam 22 is located between the lower pressure plate 17 and the upper lifting plate 16 and is in contact with both of them.

[0036] Specifically, the cam 22 can be rotated by rotating the operating shaft 21. The protrusion of the cam 22 first rotates downward to press down the lower pressure plate 17 and take a quantitative amount of water. After rotating, it continues to pull up the upper lifting plate 16. Thus, the top plug 9 can be opened first to take water and the bottom plug 8 can be opened to release water in a preset sequence. The operation is simple and fast. The operating shaft 21 is evenly distributed and can evenly press down the lower pressure plate 17 and the upper lifting plate 16, which is convenient for operation and use.

[0037] Please see Figure 8-9 The washing box 1 has a separation plate 23 fixedly connected inside, and a rotating gear 24 fixedly connected to the outer wall of the operating shaft 21. The sealing plate 19 and the rotating gear 24 are located on both sides of the separation plate 23. The outer wall of the washing box 1 has a sliding window 25. A connecting plate 26 is slidably connected inside the sliding window 25. A rack 27 is fixedly connected to the inner end of the connecting plate 26, and a push plate 28 is fixedly connected to the outer end. The rack 27 is located below the rotating gear 24 and meshes with it.

[0038] The rotation of the specific operating shaft 21 can be achieved by pushing the push plate 28 to move the rack 27, which in turn drives the rotating gear 24 that meshes with it to rotate, thereby causing the operating shaft 21 to drive the cam 22 to rotate. By pushing the push plate 28, multiple operating shafts 21 can be driven to rotate, and the rotation is converted into a push, which is more in line with user habits and more comfortable to use. The separation plate 23 separates the water storage space and the rotating gear 24 and other components, allowing a window to be opened on one side of the washing box 1 so that the connecting plate 26 can pass through, allowing the transmission components to be located inside, simplifying the appearance.

[0039] The width of the connecting plate 26 is less than the length of the sliding window 25, and in its original state, the side of the connecting plate 26 is close to one side of the sliding window 25. A floating plate 29 is elastically connected inside the sliding window 25, and the connecting plate 26 is slidably connected to the top of the floating plate 29.

[0040] The push plate 28 is pushed in two stages. When the push plate 28 is pushed, the rotating gear 24 rotates and the cam 22 rotates until the cam is pressing down on the pressure plate 17 directly downwards. You will feel a corresponding sensation on your hand. When the pressure plate 17 is pressed down to the bottom, the push plate 28 is the most difficult to push and the push is sticky. At this time, the cam 22 has rotated to the bottom. It can stay here for a short time so that the space between the metering plug 5 and the water outlet plug 4 can be filled to reduce the metering error. Then, the push plate 28 is pushed again, so that the rotating gear 24 continues to rotate. When the protrusion of the cam 22 leaves the pressure plate 17, there will be a slight jumping sensation. At this time, you can feel the cam 22 leaving the pressure plate 17 and rotating upwards. The protrusion gradually pushes the lifting plate 16 upwards, gradually reaching the sticky feeling. When the cam 22 rotates to the top, the lifting plate 16 lifts the bottom plug 8 to realize water injection. The push plate 28 is pushed to the outermost end. The cam 22 completes one revolution, realizing one cycle.

[0041] When returning to the original position, the push plate 28 is pressed down, causing the floating plate 29 to move down. At this time, the rack 27 disengages from the rotating gear 24, and can directly push the push plate 28 back to its original position. Then, the push plate 28 is released, and the floating plate 29 pushes the push plate 28 back to its original position, so that the rack 27 and the rotating gear 24 re-mesh, which facilitates the next cycle. Therefore, the exposed push plate 28 only needs to be pulled outward and pushed back to achieve the cycle of first metering, then discharging water, and returning to the original position. This greatly simplifies the operation, reduces costs, and makes it easy to control.

[0042] Please see Figure 2-6 The water separator 3 has a positioning ring 30 fixedly connected to its inner wall, a bottom plug 8 located below the positioning ring 30, a metering plug 5 slidably connected inside the water separator 3, a sealing plate 19 slidably connected inside the washing box 1, a perforation opening at the top of the washing box 1, an adjusting column 31 fixedly connected to the top of the sealing plate 19, the adjusting column 31 passing through the perforation and slidingly engaging with it, and a metering rod 15 being a telescopic rod with one end fixedly connected to the top of the pressure ring 13 and the other end fixedly connected to the bottom of the lower pressure plate 17.

[0043] The metering rod 15 includes an outer cylinder 32 and an inner rod 33. The outer cylinder 32 is fixedly connected to multiple linkage rings 34. The inner rod 33 is located inside the outer cylinder 32 and its end is rotatably connected to a forked rod 35. A linkage rod 36 is slidably connected to the middle of the inner rod 33. The bottom end of the linkage rod 36 is located above the inner end of the forked rod 35. The top end of the linkage rod 36 extends out of the top of the lower pressure plate 17 and is fixedly connected to a docking plate 37. A synchronous spring 38 is connected between the pressure ring 13 and the sealing plate 19.

[0044] The inner rod 33 has mounting grooves on both sides at its bottom end. The forked rod 35 is boomerang-shaped and is rotatably connected to the mounting groove via a torsion spring shaft. The bottom of the linkage rod 36 is fixedly connected to a conical head 39. A retaining spring 40 is connected between the lower pressure plate 17 and the sealing plate 19. A return spring 41 is connected between the docking plate 37 and the lower pressure plate 17. The elastic forces of the synchronizing spring 38, the retaining spring 40, and the return spring 41 decrease sequentially. This allows the return spring 41 to contract and press down the docking plate 37 first, then the retaining spring 40 to press down the lower pressure plate 17, and finally the synchronizing spring 38 to press down the pressure ring 13, causing it to move down. The pressure ring 13 returns first, followed by the lower pressure plate 17 and the docking plate 37, thus closing the top plug 9 first. After returning, the synchronizing spring 38 and the retaining spring 40 respectively keep the pressure ring 13 and the lower pressure plate 17 in their original positions and prevent them from falling back.

[0045] To adapt to different plate surfaces, the metered water volume needs to be adjustable. Taking increasing the water volume as an example, by lifting the adjusting column 31 upwards, the sealing plate 19 and the metering plug 5 can be moved upwards, thereby increasing the space between the metering plug 5 and the outlet plug 4, and increasing the metered water volume. At the same time, the pressure ring 13 and the outer cylinder 32 move upwards together with the metering plug 5, and the relative distance between the three remains unchanged. Instead, the inner rod 33 gradually enters the outer cylinder 32. Therefore, even if the metered volume is adjusted and the position of the metering plug 5 is moved, the rotation of the cam 22 can still open the top plug 9 at the original downward pressure distance, thus enabling water to enter.

[0046] Please see Figure 5-6 Specifically, during adjustment, the forked rod 35 at the bottom of the inner rod 33 is in a constricted state. At this time, the outer cylinder 32 and the inner rod 33 can slide relative to each other. When the metering plug 5 and the outer cylinder 32 move upward, the inner rod 33 remains in its original position, while the portion gradually entering the outer cylinder 32 increases. When water needs to be added after adjustment, the rotation of the cam 22 is still used. The cam 22 rotates downward first and contacts the docking plate 37, which then presses down the docking plate 37, thereby driving the linkage rod 36 to move downward. This, in turn, presses down the inner end of the forked rod 35, causing its outer end to open. The bottom end of the opened forked rod 35 will then press against the linkage ring 34 or tighten and squeeze the linkage ring 34. At this time, the connection between the inner rod 33 and the outer cylinder 32 is achieved. Under the action of the opened forked rod 35, the inner rod 33 moves downward, which can drive the outer cylinder 32 and the bottom pressure ring 13 to move downward.

[0047] After the inner rod 33 and the outer cylinder 32 are connected, the cam 22 continues to rotate, which can press down the lower pressure plate 17 together with the docking plate 37. Thus, by pressing down the lower pressure plate 17, the upper push rod 12 is driven to open the top plug 9, so as to realize quantitative water intake. Based on this structure, the quantitative water intake can be achieved without changing the internal structure. The adjustment is convenient and simple, and it can adapt to the washing needs of various board surfaces, thereby expanding the scope of application and enhancing its versatility.

[0048] Please see Figure 2 The inlet pipe 20 is fixedly connected to a positioning leak plate 42 and a sealing ring 43. The positioning leak plate 42 is located at the bottom of the sealing ring 43 and a sealing plate 44 is provided between the two. The diameter of the sealing plate 44 is larger than the inner diameter of the sealing ring 43 and smaller than the inner diameter of the inlet pipe 20.

[0049] In order to quickly and evenly fill the space between each metering plug 5 and the water outlet plug 4 in a short time, when water is introduced, as long as the water inside the washing tank 1 is not full, the sealing plate 44 will fall back to the bottom, and then the water inlet pipe 20 will be opened to let water in and fill the washing tank 1. This ensures that the space inside the washing tank 1 is always full. In this way, even if the tank is tilted at any angle during use, as long as it is not inverted, the water will be able to fill the space between each metering plug 5 and the water outlet plug 4 evenly and quickly, thereby reducing water injection errors. The operation will not affect the metering results when the water is metered by gravity, which is more in line with the actual application requirements and reduces the difficulty of operation.

[0050] Please see Figure 1 , 7 The bottom of the washing tank 1 is rotatably connected to multiple protective rods 45. The bottom of the protective rods 45 is fixedly connected to a flat bottom plate 46. A water-absorbing pad is installed on the top of the flat bottom plate 46. The flat bottom plate 46 is used to block the guide nozzle 2.

[0051] When not in use or after each row of water has been filled, the washing box 1 can be tilted so that the protective rod 45 rotates under gravity, causing the flat bottom plate 46 to rotate to the bottom of the guide nozzle 2, thereby absorbing the residual water droplets through the water-absorbing pad above it. This prevents water from dripping during use and protects the guide nozzle 2 from external contamination when not in use.

[0052] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0053] The preferred embodiments of the present invention disclosed above are merely illustrative of the invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the invention to any specific implementation. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of the invention, thereby enabling those skilled in the art to better understand and utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. An enzyme-linked immunosorbent assay (ELISA) plate washer, characterized in that: The system includes a washing tank (1), with multiple guide nozzles (2) connected to the bottom of the washing tank (1). Each guide nozzle (2) is fixedly connected to a water distribution cylinder (3) at its top. The water distribution cylinder (3) is fixedly connected to the bottom of the washing tank (1). The bottom of the water distribution cylinder (3) is provided with a water outlet plug (4). A metering plug (5) is installed inside the water distribution cylinder (3). A through hole (6) is opened between the metering plug (5) and the water outlet plug (4). A sliding cylinder (7) is provided between the metering plug (5) and the water outlet plug (4). A bottom plug (8) and a top plug (9) are slidably connected to the outside of the sliding cylinder (7). The top plug (9) and the bottom plug (8) are both frustoconical. The tip of the top plug (9) is facing upwards and located at the bottom of the metering plug (5). The tip of the bottom plug (8) is facing downwards and located above the water outlet plug (4). (9) A sealing spring (10) is provided between them. A water discharge rod (11) is slidably connected in the middle of the slide cylinder (7). An upper top rod (12) is fixedly connected to the bottom of the water discharge rod (11). A pressure ring (13) is elastically connected above the metering plug (5). Multiple pressure rods (14) are fixedly connected to the bottom of the pressure ring (13). A metering rod (15) is fixedly connected to the top of the pressure ring (13). An upper lifting plate (16) is fixedly connected to the top of the water discharge rod (11). A lower pressure plate (17) is fixedly connected to the top of the metering rod (15). A connecting cylinder (18) is fixedly connected to the top of the connecting cylinder (18). A sealing plate (19) is fixedly connected to the top of the connecting cylinder (18). The sealing plate (19) is used to store water between itself and the washing box (1). A water inlet pipe (20) is connected to the top of the washing box (1).

2. The enzyme-linked immunosorbent assay (ELISA) plate washer according to claim 1, characterized in that: The lower pressure plate (17) is located below the upper lifting plate (16). An operating shaft (21) is rotatably connected inside the washing box (1). A cam (22) is fixedly connected to the outer wall of the operating shaft (21). The cam (22) is located between the lower pressure plate (17) and the upper lifting plate (16) and is in contact with both.

3. The enzyme-linked immunosorbent assay (ELISA) plate washer according to claim 2, characterized in that: A separation plate (23) is fixedly connected inside the washing box (1). A rotating gear (24) is fixedly connected to the outer wall of the operating shaft (21). The sealing plate (19) and the rotating gear (24) are located on both sides of the separation plate (23). A sliding window (25) is opened on the outer wall of the washing box (1). A connecting plate (26) is slidably connected inside the sliding window (25). A rack (27) is fixedly connected to the inner end of the connecting plate (26) and a push plate (28) is fixedly connected to the outer end. The rack (27) is located below the rotating gear (24) and meshes with it.

4. The enzyme-linked immunosorbent assay (ELISA) plate washer according to claim 3, characterized in that: The width of the connecting plate (26) is less than the length of the sliding window (25), and in its original state, the side of the connecting plate (26) is close to one side of the sliding window (25). A floating plate (29) is elastically connected inside the sliding window (25), and the connecting plate (26) is slidably connected to the top of the floating plate (29).

5. The enzyme-linked immunosorbent assay (ELISA) plate washer according to claim 1, characterized in that: The inner wall of the water distribution cylinder (3) is fixedly connected to a positioning ring (30), the bottom plug (8) is located below the positioning ring (30), the metering plug (5) is slidably connected inside the water distribution cylinder (3), the sealing plate (19) is slidably connected inside the washing box (1), the washing box (1) has a perforation at the top, the sealing plate (19) is fixedly connected to an adjusting column (31), the adjusting column (31) passes through the perforation and slides with it, the metering rod (15) is a telescopic rod and one end is fixedly connected to the top of the pressure ring (13) and the other end is fixedly connected to the bottom of the lower pressure plate (17).

6. The enzyme-linked immunosorbent assay (ELISA) plate washer according to claim 5, characterized in that: The metering rod (15) includes an outer cylinder (32) and an inner rod (33). The outer cylinder (32) is fixedly connected with multiple linkage rings (34). The inner rod (33) is located inside the outer cylinder (32) and its end is rotatably connected with a forked rod (35). The middle part of the inner rod (33) is slidably connected with a linkage rod (36). The bottom end of the linkage rod (36) is located above the inner end of the forked rod (35). The top end of the linkage rod (36) extends out of the top of the lower pressure plate (17) and is fixedly connected with a docking plate (37). A synchronous spring (38) is connected between the pressure ring (13) and the sealing plate (19).

7. The enzyme-linked immunosorbent assay (ELISA) plate washer according to claim 6, characterized in that: The inner rod (33) has mounting grooves on both sides of its bottom end. The forked rod (35) is boomerang-shaped and is rotatably connected to the mounting groove via a torsion spring shaft. The bottom of the linkage rod (36) is fixedly connected to a conical head (39). A retaining spring (40) is connected between the lower pressure plate (17) and the sealing plate (19). A return spring (41) is connected between the docking plate (37) and the lower pressure plate (17). The elasticity of the synchronization spring (38), the retaining spring (40), and the return spring (41) decreases sequentially.

8. The enzyme-linked immunosorbent assay (ELISA) plate washer according to claim 1, characterized in that: The water inlet pipe (20) is fixedly connected to a positioning leak plate (42) and a sealing ring (43). The positioning leak plate (42) is located at the bottom of the sealing ring (43) and a sealing plate (44) is provided between them. The diameter of the sealing plate (44) is larger than the inner diameter of the sealing ring (43) and smaller than the inner diameter of the water inlet pipe (20).

9. The enzyme-linked immunosorbent assay (ELISA) plate washer according to claim 1, characterized in that: The bottom of the washing box (1) is rotatably connected to multiple protective rods (45), and the bottom of the protective rods (45) is fixedly connected to a flat bottom plate (46). The top of the flat bottom plate (46) is equipped with a water-absorbing pad, and the flat bottom plate (46) is used to block the guide nozzle (2).

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