Microfluidic detection device

Abstract

The application provides a microfluidic detection device, which comprises a reagent box, a microfluidic chip located below the reagent box, wherein the microfluidic chip is provided with a detection cavity, a sample adding port and a detection adding port which are in communication with the detection cavity, a sealing tube comprising a sealing tube body and a blocking piece movably penetrating the sealing tube body, wherein the sealing tube body is arranged on the reagent box and is provided with a sealing liquid cavity and a liquid outlet which is in communication with the lower end of the sealing liquid cavity, the liquid outlet is located above the detection adding port and is in communication with the detection adding port, the upper end of the blocking piece penetrates the sealing liquid cavity upward, and the lower end of the blocking piece is located in the sealing liquid cavity and is provided with a blocking position for blocking the liquid outlet and an opening position for opening the liquid outlet. Through the technical scheme, the problems of complicated operation, time and labor consumption and easy errors in the related art caused by manually adding multiple reagents can be solved.

Description

Technical Field

[0001] This invention relates to the field of microfluidics, and more specifically, to a microfluidic detection device. Background Technology

[0002] Microfluidic slide technology employs an integrated detection method. First, different detection reagents are pre-loaded into sealed tubes. Then, the microfluidic slide is controlled by a matching instrument. The microfluidic slide enables complex operations such as reaction, separation, and detection of reagents between different sealed tubes. Finally, the detection process is completed sequentially according to the experimental order.

[0003] In related technologies, the use of microfluidic carriers generally requires the manual addition of multiple reagents. The reagents in the kit are drawn up with a pipette and then injected into the microfluidic carrier.

[0004] However, the manual addition of multiple reagents in related technologies is cumbersome, time-consuming, labor-intensive, and prone to errors. Summary of the Invention

[0005] This invention provides a microfluidic detection device to solve the problems of cumbersome operation, time-consuming and labor-intensive operation, and easy error in the manual addition of multiple reagents in related technologies.

[0006] This invention provides a microfluidic detection device, comprising: a reagent kit; a microfluidic carrier located below the reagent kit, the microfluidic carrier having a detection cavity and a sample inlet and a detection inlet connected to the detection cavity; and a sealing tube including a sealing tube body and a sealing element movably inserted through the sealing tube body. The sealing tube body is disposed on the reagent kit and has a sealing liquid cavity and an outlet connected to the lower end of the sealing liquid cavity. The outlet is located above and connected to the detection inlet. The upper end of the sealing element extends upward through the sealing liquid cavity, and the lower end of the sealing element is located within the sealing liquid cavity and has a sealing position for sealing the outlet and an opening position for opening the outlet.

[0007] Furthermore, the sealing tube body includes a tube body and a sealing cap covering the upper end of the tube body, a sealing liquid chamber is provided in the tube body, a liquid outlet is provided at the lower end of the tube body, and a sealing element is movably inserted through the sealing cap.

[0008] Furthermore, the sealing component includes a sealing rod and a sealing head disposed at the lower end of the sealing rod. The sealing rod is movably inserted into the sealing cover, and the sealing head has a sealing position for sealing the liquid outlet and an opening position for opening the liquid outlet.

[0009] Furthermore, the pipe body includes a first pipe section and a second pipe section connected to the lower end of the first pipe section. A sealing cap is placed on the upper end of the first pipe section, and the liquid outlet is located at the lower end of the second pipe section. The cross-sectional dimensions of the second pipe section gradually decrease from top to bottom.

[0010] Furthermore, the sealing component includes a sealing rod and a sealing head disposed at the lower end of the sealing rod. Both the sealing head and the second pipe section have a conical structure, and the taper of the sealing head is the same as that of the second pipe section.

[0011] Furthermore, an operating boss is provided on the side wall of the sealing rod, and the operating boss is located above the sealing cover.

[0012] Furthermore, the reagent kit includes a perimeter plate, a top plate, and a support plate. The upper end of the perimeter plate is connected to the outer edge of the top plate, the lower end of the perimeter plate is connected to the microfluidic carrier plate, the outer edge of the support plate is connected to the inner sidewall of the perimeter plate, and the main body of the sealing tube passes through the top plate and the support plate.

[0013] Furthermore, both the top plate and the support plate have a first clearance notch located above the sample inlet; and / or, the microfluidic carrier also has a waste outlet connected to the detection chamber, and both the top plate and the support plate have a second clearance notch located above the waste outlet.

[0014] Furthermore, the microfluidic detection device also includes a drive unit that is driven to connect with the plugging component, the drive unit being able to drive the plugging component to move between a plugging position and an open position.

[0015] Furthermore, the sealing tube includes a sealing liquid tube and multiple reagent liquid tubes; the microfluidic carrier has a pad located on the outside of the reagent kit and an electrode located in the detection chamber, the pad being electrically connected to the electrode; a first annular protrusion is provided above the detection liquid inlet, the first annular protrusion surrounding the outside of the sealing tube body; a second annular protrusion is provided above the sample liquid inlet, the second annular protrusion surrounding the outside of the sample liquid inlet.

[0016] According to the technical solution of this invention, the microfluidic detection device includes a reagent kit, a microfluidic carrier, and a sealing tube. The microfluidic carrier is positioned below the reagent kit, and the sealing tube is positioned on top of the reagent kit, such that the outlet of the sealing tube is connected to the detection inlet, facilitating the injection of reagent from the sealing tube into the detection chamber of the microfluidic carrier to complete the sample detection process. The sealing tube body is used to pre-fill the reagent, and the sealed liquid chamber within the sealing tube body provides sealed storage for the reagent. A plugging element further seals the outlet, preventing reagent leakage when no liquid is being added. When the plugging element is in the plugged position, its lower end seals the outlet, preventing reagent leakage and providing sealed storage. When reagent is needed to detect the sample, the plugging element moves from the plugged position to the open position, opening the outlet at the lower end of the sealing tube body. The reagent then flows into the detection chamber through the outlet, where the reagent droplets undergo subsequent manipulation, processing, and the corresponding biochemical reaction. By adopting the above structure and integrating the sealed tube onto the reagent kit, the detection steps can be simplified, and the reaction efficiency and accuracy of the biochemical reaction can be improved. Attached Figure Description

[0017] The accompanying drawings, which form part of this application, are used to provide a further understanding of the invention. The illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an undue limitation of the invention. In the drawings:

[0018] Figure 1 A schematic diagram of the structure of a microfluidic detection device provided according to an embodiment of the present invention is shown;

[0019] Figure 2 An exploded view of a microfluidic detection device provided according to an embodiment of the present invention is shown;

[0020] Figure 3 A side view of a microfluidic detection device provided according to an embodiment of the present invention is shown;

[0021] Figure 4 It shows Figure 3 Sectional view at point AA;

[0022] Figure 5 A schematic diagram of the structure of the sealing tube of the microfluidic detection device provided according to an embodiment of the present invention is shown.

[0023] The above figures include the following reference numerals:

[0024] 10. Reagent kit; 11. Enclosure; 12. Top plate; 13. Support plate; 14. First clearance notch; 15. Second clearance notch;

[0025] 20. Microfluidic carrier plate; 21. Detection chamber; 22. Sample inlet; 221. Second annular boss; 23. Detection inlet; 231. First annular boss; 24. Waste outlet; 25. Pad;

[0026] 30. Sealing tube; 31. Sealing tube body; 311. Sealing liquid chamber; 312. Liquid outlet; 313. Tube body; 3131. First tube section; 3132. Second tube section; 314. Sealing cap; 32. Sealing component; 321. Sealing rod; 3211. Operating boss; 322. Sealing head; 33. Sealing liquid tube; 34. Reagent liquid tube. Detailed Implementation

[0027] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. The following description of at least one exemplary embodiment is merely illustrative and is in no way intended to limit the present invention or its application or use. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0028] like Figures 1 to 5 As shown, this embodiment of the invention provides a microfluidic detection device, which includes a reagent kit 10, a microfluidic carrier 20, and a sealing tube 30. The microfluidic carrier 20 is located below the reagent kit 10 and has a detection chamber 21 and a sample inlet 22 and a detection inlet 23 connected to the detection chamber 21. The sealing tube 30 includes a sealing tube body 31 and a plugging member 32 movably inserted into the sealing tube body 31. The sealing tube body 31 is disposed on the reagent kit 10 and has a sealing liquid chamber 311 and an outlet 312 connected to the lower end of the sealing liquid chamber 311. The outlet 312 is located above the detection inlet 23 and is connected to the detection inlet 23. The upper end of the plugging member 32 extends upward through the sealing liquid chamber 311, and the lower end of the plugging member 32 is located inside the sealing liquid chamber 311 and has a blocking position for blocking the outlet 312 and an opening position for opening the outlet 312.

[0029] The microfluidic detection device provided in this embodiment includes a reagent kit 10, a microfluidic carrier 20, and a sealing tube 30. The microfluidic carrier 20 is positioned below the reagent kit 10, and the sealing tube 30 is positioned on top of the reagent kit 10, such that the outlet 312 of the sealing tube 30 is connected to the detection inlet 23, facilitating the injection of reagent from the sealing tube 30 into the detection chamber 21 of the microfluidic carrier 20 to complete the sample detection process. The sealing tube body 31 within the sealing tube 30 is used to pre-fill the reagent. The sealed liquid chamber 311 within the sealing tube body 31 provides a sealed storage for the reagent, and the sealing element 32 blocks the outlet 312 to prevent reagent leakage from the sealing tube 30 when no liquid is being added. When the sealing element 32 is in the sealed position, its lower end seals the outlet 312, preventing reagent from flowing out and thus providing a sealed storage for the reagent. When the reagent is needed to test the sample, the sealing element 32 moves, changing from the sealed position to the open position, opening the outlet 312 at the lower end of the sealed tube body 31. The reagent then flows into the detection chamber 21 through the outlet 312, where the reagent droplets undergo subsequent manipulation, processing, and the corresponding biochemical reaction. By integrating the sealed tube 30 onto the reagent kit 10, the detection steps are simplified, and the reaction efficiency and accuracy of the biochemical reaction are improved.

[0030] like Figure 2 , Figure 4 as well as Figure 5 As shown, the sealed tube body 31 includes a tube body 313 and a sealing cap 314 covering the upper end of the tube body 313. A sealing liquid chamber 311 is disposed in the tube body 313, and a liquid outlet 312 is disposed in the lower end of the tube body 313. A sealing element 32 is movably inserted through the sealing cap 314. With the above structure, the sealing liquid chamber 311 in the tube body 313 is used to seal the reagent, and the sealing cap 314 can be placed on the tube body 313. When the sealing cap 314 is opened, the reagent can be added into the sealed tube body 31. Furthermore, the sealing element 32 can be movably inserted through the sealing cap 314, allowing the sealing element 32 to switch between the sealing position and the open position. This allows the sealing tube body 31 to be controlled by manipulating the sealing element 32, facilitating operation control and avoiding the problem of frequent manual reagent addition.

[0031] like Figure 2 , Figure 4 as well as Figure 5As shown, the sealing element 32 includes a sealing rod 321 and a sealing head 322 disposed at the lower end of the sealing rod 321. The sealing rod 321 is movably inserted through the sealing cover 314, and the sealing head 322 has a sealing position for blocking the liquid outlet 312 and an opening position for opening the liquid outlet 312. With this structure, the sealing rod 321 is movably inserted through the sealing cover 314, facilitating its sliding on the cover. The sealing head 322, located at the lower end of the sealing rod 321, seals the liquid outlet 312, ensuring the reliability of the sealing structure of the sealing element 32.

[0032] like Figure 2 , Figure 4 as well as Figure 5 As shown, the tube body 313 includes a first tube segment 3131 and a second tube segment 3132 connected to the lower end of the first tube segment 3131. A sealing cap 314 is placed over the upper end of the first tube segment 3131, and an outlet 312 is located at the lower end of the second tube segment 3132. The cross-sectional dimensions of the second tube segment 3132 gradually decrease from top to bottom. By designing the tube body 313 as a first tube segment 3131 and a second tube segment 3132, and by having the cross-sectional dimensions of the second tube segment 3132 gradually decrease from top to bottom, the tube wall of the second tube segment 3132 has a certain slope, which facilitates reagent outflow. Furthermore, the connection between the lower end of the first tube segment 3131 and the second tube segment 3132 ensures reagent outflow while increasing the reagent storage space.

[0033] like Figure 2 and Figure 4 As shown, the sealing component 32 includes a sealing rod 321 and a sealing head 322 disposed at the lower end of the sealing rod 321. Both the sealing head 322 and the second pipe section 3132 have a conical structure, and the taper of the sealing head 322 is the same as that of the second pipe section 3132. With the above structure, the sealing head 322 can cooperate with the second pipe section 3132, so that the sealing head 322 can achieve the sealing function at the sealing position. Since the taper of the sealing head 322 is the same as that of the second pipe section 3132, it can not only facilitate the sealing of the reagent, but also facilitate the sealing head 322 to be sealed above the liquid outlet 312.

[0034] like Figure 4 and Figure 5 As shown, an operating boss 3211 is provided on the side wall of the sealing rod 321, and the operating boss 3211 is located above the sealing cover 314. By providing the operating boss 3211 on the sealing rod 321, it is convenient to grasp and move the sealing rod 321, and it can also play a limiting role for the grasping device, so as to avoid the sealing rod 321 being unable to be changed from the sealing position to the open position due to the low friction when grasping the sealing rod 321.

[0035] like Figure 1and Figure 2 As shown, the reagent kit 10 includes a surrounding plate 11, a top plate 12, and a support plate 13. The upper end of the surrounding plate 11 is connected to the outer edge of the top plate 12, and the lower end of the surrounding plate 11 is connected to the microfluidic carrier 20. The outer edge of the support plate 13 is connected to the inner wall of the surrounding plate 11. The sealing tube body 31 passes through the top plate 12 and the support plate 13. With the above structure, the surrounding plate 11, the top plate 12, and the support plate 13 together constitute the reagent kit 10. The surrounding plate 11 is connected to the microfluidic carrier 20, which allows the reagent kit 10 to protect the microfluidic carrier 20 and prevent the reagents in the microfluidic carrier 20 from being contaminated. The top plate 12 and the support plate 13 can improve the structural strength of the reagent kit 10. The sealing tube body 31 passing through the top plate 12 and the support plate 13 allows the sealing tube body 31 to be replaced, improving the reliability of the detection reagents in the reagent kit 10.

[0036] It should be noted that the lower end of the enclosure 11 is connected to the microfluidic carrier 20. The connection refers to the fixed connection between the enclosure 11 and the microfluidic carrier 20, which facilitates the fixation of the reagent kit 10 and makes detection convenient. Alternatively, it can be a detachable connection, which facilitates the disassembly and assembly of the reagent kit 10 and expands the applicability of the device.

[0037] like Figure 1 and Figure 2 As shown, both the top plate 12 and the support plate 13 have a first clearance notch 14, which is located above the sample filling port 22. By setting the first clearance notch 14, it is convenient to add the sample to be tested into the sample filling port 22, which facilitates operation.

[0038] like Figure 1 and Figure 2 As shown, the microfluidic carrier 20 also has a waste liquid outlet 24 connected to the detection chamber 21. Both the top plate 12 and the support plate 13 have second clearance notches 15, located above the waste liquid outlet 24. With this structure, the reagent flowing from the outlet 312 enters the detection chamber 21 and undergoes a biochemical reaction with the sample to be tested. The second clearance notches 15 on the top plate 12 and the support plate 13 facilitate the removal of the generated waste liquid from the waste liquid outlet 24, thereby improving the detection efficiency of the microfluidic detection device.

[0039] In this embodiment, the microfluidic detection device also includes a driving component connected to the sealing component 32. The driving component can move the sealing component 32 between a sealing position and an open position. By providing the driving component, the driving component can move the sealing rod 321, thereby controlling the sealing process of the sealing component 32 in sealing or opening the liquid outlet 312. This facilitates automated control and avoids operational errors caused by manual reagent addition. The driving component includes a robotic arm and other driving structures.

[0040] In this embodiment, the reagent kit 10 and the microfluidic carrier 20 are integrally molded. This structure reduces the connection steps between the reagent kit 10 and the microfluidic carrier 20, and improves the reliability of the microfluidic detection device, facilitating direct detection and making the operation process more stable.

[0041] It should be noted that the reagent kit 10 and the microfluidic carrier 20 can be separate structures, which facilitates the processing and production of the reagent kit 10 and makes it easy to replace the microfluidic carrier 20.

[0042] like Figure 1 As shown, the sealing tube 30 includes a sealing liquid tube 33 and multiple reagent liquid tubes 34. With this structure, after adding reagents, the sealing liquid is added to the sealing liquid tube 33. This facilitates the flow of reagents into the microfluidic carrier 20 while preventing the introduction of air bubbles that could affect the detection results. Furthermore, the multiple reagent liquid tubes 34 allow for the addition of different types of reagents from the kit 10, enabling various detection experiments and enhancing the practicality of the microfluidic detection device.

[0043] like Figure 1 , Figure 2 as well as Figure 4 As shown, the microfluidic carrier 20 has a pad 25 located on the outside of the reagent kit 10 and an electrode located within the detection chamber 21. The pad 25 is electrically connected to the electrode. With the above structure, the pad 25 is electrically connected to the electrode, so that the reagent flowing into the detection chamber 21 flows under the influence of the electrode being energized.

[0044] Specifically, the hydrophobicity of reagent droplets within the microfluidic carrier 20 can also be controlled by switching the voltage on and off. This refers to the electrowetting phenomenon on a dielectric. For example, when no voltage is applied to the electrodes, the surface remains hydrophobic, and the reagent droplets form spherical droplets with a larger contact angle. When an electric field is applied, a polarized hydrophilic surface is formed. The reagent droplets then flatten and the contact angle decreases. By controlling this switching of the voltage on and off, we can create an interfacial tension gradient, thereby allowing controlled displacement of the droplets on the microfluidic carrier 20. Due to the presence of the electrodes, an electric field can be applied to specific locations on the reagent droplets. The change in the contact angle of the reagent droplets causes an internal pressure imbalance, thereby driving the reagent droplets to move, thus achieving highly programmable droplet control.

[0045] Electrowetting (EW) refers to the phenomenon where applying voltage to the pads 25 and electrodes alters the wettability of a reagent droplet, i.e., changes the contact angle, causing the droplet to deform and shift. Wetting, in general, refers to the process by which one fluid replaces another on a solid surface. Wetting occurs when a liquid spreads on a solid surface, and the solid-liquid interface tends to expand, meaning the liquid's adhesion to the solid surface is greater than its cohesive force. Conversely, nonwetting occurs when a liquid cannot spread on a solid surface, and the interface tends to shrink into a spherical shape; nonwetting means the liquid's adhesion to the solid surface is less than its cohesive force.

[0046] It should be noted that, since the pad 25 is electrically connected to the electrode, supplying power to the pad 25 turns on the voltage of the electrode corresponding to the outlet 312. This reduces the hydrophobic angle of the reagent droplets on the microfluidic carrier 20, causing them to adhere to the surface. This prevents the reagent droplets from being moved when the sealing liquid is added later. Once all the reagents have flowed into the microfluidic carrier 20, the sealing component 32 of the sealing liquid tube 33 is lifted upwards using a driving device, allowing the sealing liquid to flow into the microfluidic carrier 20. Then, the sample to be tested is injected into the sample inlet 22, and the detection instrument is turned on to complete the sample processing or detection through a specific program script.

[0047] like Figure 1 and Figure 2 As shown, a first annular protrusion 231 is provided above the detection liquid inlet 23, and the first annular protrusion 231 surrounds the outside of the sealing tube body 31. By providing the first annular protrusion 231, the detection liquid inlet 23 is easily protected, preventing other reagents from entering through the detection liquid inlet 23 or reagents from overflowing, thereby contaminating the reagents and affecting the detection effect.

[0048] like Figure 1 and Figure 2 As shown, a second annular protrusion 221 is provided above the sample filling port 22, and the second annular protrusion 221 surrounds the outside of the sample filling port 22. By providing the second annular protrusion 221, the sample filling port 22 is easily protected, preventing other reagents from entering through the sample filling port 22 or the sample from overflowing, thereby contaminating the sample reagents and affecting the detection effect.

[0049] The apparatus provided by the embodiments has the following beneficial effects:

[0050] (1) By integrating the sealing tube 30 onto the kit 10, the detection steps can be simplified and the reaction efficiency of the biochemical reaction can be improved.

[0051] (2) Liquid is added to the sealing tube body 31 by manipulating the sealing component 32, which facilitates operation control and avoids the problem of frequent manual addition of reagents.

[0052] (3) The enclosure plate 11, the top plate 12 and the support plate 13 together constitute the reagent kit 10. The enclosure plate 11 is connected to the microfluidic carrier 20, so that the reagent kit 10 can protect the microfluidic carrier 20 and prevent the reagents in the microfluidic carrier 20 from being contaminated. The top plate 12 and the support plate 13 can improve the structural strength of the reagent kit 10.

[0053] (4) By setting a driving component, the driving component can drive the sealing rod 321 to move, thereby controlling the sealing component 32 to seal or open the liquid outlet 312, which facilitates automated control and avoids operational errors caused by manually adding reagents.

[0054] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to this application. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0055] Unless otherwise specifically stated, the relative arrangement, numerical expressions, and values ​​of the components and steps set forth in these embodiments do not limit the scope of the invention. It should also be understood that, for ease of description, the dimensions of the various parts shown in the drawings are not drawn to actual scale. Techniques, methods, and devices known to those skilled in the art may not be discussed in detail, but where appropriate, such techniques, methods, and devices should be considered part of the specification. In all examples shown and discussed herein, any specific values ​​should be interpreted as merely exemplary and not as limitations. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar reference numerals and letters in the following figures denote similar items; therefore, once an item is defined in one figure, it need not be further discussed in subsequent figures.

[0056] In the description of this invention, it should be understood that the orientation or positional relationship indicated by directional terms such as "front, back, up, down, left, right", "horizontal, vertical, horizontal" and "top, bottom" is generally based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing this invention and simplifying the description. Unless otherwise stated, these directional terms do not indicate or imply that the device or element referred to must have a specific orientation or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on the scope of protection of this invention; the directional terms "inner" and "outer" refer to the inner and outer contours relative to the outline of each component itself.

[0057] For ease of description, spatial relative terms such as "above," "on top of," "on the upper surface of," "above," etc., are used herein to describe the spatial positional relationship of a device or feature as shown in the figures to other devices or features. It should be understood that spatial relative terms are intended to encompass different orientations in use or operation beyond the orientation of the device as described in the figures. For example, if the device in the figures were inverted, a device described as "above" or "on top of" other devices or structures would subsequently be positioned as "below" or "under" other devices or structures. Thus, the exemplary term "above" can include both "above" and "below." The device may also be positioned in other different ways (rotated 90 degrees or in other orientations), and the spatial relative descriptions used herein will be interpreted accordingly.

[0058] Furthermore, it should be noted that the use of terms such as "first" and "second" to define components is merely for the purpose of distinguishing the corresponding components. Unless otherwise stated, the above terms have no special meaning and therefore should not be construed as limiting the scope of protection of this invention.

[0059] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A microfluidic detection device, characterized in that, The microfluidic detection device includes: Reagent kit (10); A microfluidic carrier plate (20) is located below the kit (10). The microfluidic carrier plate (20) has a detection chamber (21) and a sample dispensing port (22) and a detection dispensing port (23) connected to the detection chamber (21). The sealing tube (30) includes a sealing tube body (31) and a plug (32) movably inserted through the sealing tube body (31). The sealing tube body (31) is replaceably disposed on the reagent kit (10). The sealing tube body (31) has a sealing liquid chamber (311) and an outlet (312) connected to the lower end of the sealing liquid chamber (311). The outlet (312) is located above the detection liquid inlet (23) and is connected to the detection liquid inlet (23). The upper end of the plug (32) extends upward through the sealing liquid chamber (311), and the lower end of the plug (32) is located inside the sealing liquid chamber (311) and has a blocking position for blocking the outlet (312) and an opening position for opening the outlet (312).

2. The microfluidic detection device according to claim 1, characterized in that, The sealing tube body (31) includes a tube body (313) and a sealing cap (314) covering the upper end of the tube body (313). The sealing liquid chamber (311) is located in the tube body (313), the liquid outlet (312) is located at the lower end of the tube body (313), and the sealing member (32) is movably inserted through the sealing cap (314).

3. The microfluidic detection device according to claim 2, characterized in that, The sealing component (32) includes a sealing rod (321) and a sealing head (322) disposed at the lower end of the sealing rod (321). The sealing rod (321) is movably inserted through the sealing cover (314). The sealing head (322) has a sealing position for sealing the liquid outlet (312) and an opening position for opening the liquid outlet (312).

4. The microfluidic detection device according to claim 2, characterized in that, The tube body (313) includes a first tube segment (3131) and a second tube segment (3132) connected to the lower end of the first tube segment (3131). The sealing cap (314) is placed on the upper end of the first tube segment (3131), and the liquid outlet (312) is located at the lower end of the second tube segment (3132). The cross-sectional dimensions of the second tube segment (3132) gradually decrease from top to bottom.

5. The microfluidic detection device according to claim 4, characterized in that, The sealing component (32) includes a sealing rod (321) and a sealing head (322) disposed at the lower end of the sealing rod (321). The sealing head (322) and the second pipe section (3132) are both conical structures, and the taper of the sealing head (322) is the same as that of the second pipe section (3132).

6. The microfluidic detection device according to claim 3, characterized in that, An operating boss (3211) is provided on the side wall of the sealing rod (321), and the operating boss (3211) is located above the sealing cover (314).

7. The microfluidic detection device according to any one of claims 1 to 6, characterized in that, The kit (10) includes a surrounding plate (11), a top plate (12), and a support plate (13). The upper end of the surrounding plate (11) is connected to the outer edge of the top plate (12), the lower end of the surrounding plate (11) is connected to the microfluidic carrier (20), the outer edge of the support plate (13) is connected to the inner wall of the surrounding plate (11), and the sealing tube body (31) passes through the top plate (12) and the support plate (13).

8. The microfluidic detection device according to claim 7, characterized in that, Both the top plate (12) and the support plate (13) have a first clearance notch (14) located above the sample filling port (22); and / or, The microfluidic carrier plate (20) also has a waste liquid port (24) connected to the detection chamber (21), and both the top plate (12) and the support plate (13) have a second clearance notch (15), which is located above the waste liquid port (24).

9. The microfluidic detection device according to any one of claims 1 to 6, characterized in that, The microfluidic detection device also includes a driving component that is drivenly connected to the plugging component (32), the driving component being able to drive the plugging component (32) to move between the plugging position and the opening position.

10. The microfluidic detection device according to any one of claims 1 to 6, characterized in that, The sealing tube (30) includes a sealing liquid tube (33) and multiple reagent liquid tubes (34). The microfluidic carrier (20) has a pad (25) located on the outside of the kit (10) and an electrode located in the detection cavity (21), the pad (25) being electrically connected to the electrode; A first annular protrusion (231) is provided above the detection liquid inlet (23), and the first annular protrusion (231) surrounds the outside of the sealing tube body (31); A second annular protrusion (221) is provided above the sample inlet (22), and the second annular protrusion (221) surrounds the outside of the sample inlet (22).

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