An antibacterial and angiogenic nylon 6 material and a preparation method thereof

Abstract

The present invention belongs to the field of biofunctional materials, and discloses an antibacterial and angiogenic nylon 6 material and a preparation method thereof, and the specific steps are as follows: (1) 3D printing nylon 6 powder to obtain a 3D printed nylon 6 scaffold; (2) soaking the nylon 6 scaffold obtained in step (1) in a dopa compound and an angiogenic protein aqueous solution, adjusting the pH, heating and stirring the reaction, and obtaining an angiogenic nylon 6 scaffold; (3) soaking the angiogenic nylon 6 scaffold obtained in step (2) in an amino-containing antibacterial drug and a phosphate group-containing compound aqueous solution, heating and cooling to obtain an antibacterial and angiogenic nylon 6 scaffold. The present invention modifies the surface of the nylon 6 scaffold layer by layer, and the obtained nylon 6 scaffold material has a uniform surface, good antibacterial and angiogenic activity, and has a comprehensive performance far superior to that of the unmodified nylon 6 material, and has a wide range of application prospects in the field of bone repair and bone regeneration in medical treatment.

Description

Technical Field

[0001] The invention belongs to the technical field of biofunctional materials, and in particular relates to an antibacterial and angiogenesis-promoting nylon 6 material and a preparation method thereof. Background Art

[0002] Nylon 6 material has excellent mechanical properties, chemical stability and controllable biocompatibility, which make it a potential biomedical material and is widely used in wound dressings, bone repair materials and other fields.

[0003] On the one hand, nylon 6 can be modified by introducing bioactive compounds, adjusting the surface properties of the material, and optimizing the degradation performance. The modification can improve its mechanical properties, making it more tough and wear-resistant, and can enhance the mechanical properties of biomaterials for use in load and support tissue engineering, bone repair, etc. On the other hand, nylon 6 can improve its reaction accessibility and surface activity through chemical modification, which can combine the advantages and characteristics of nylon 6 and provide new possibilities for innovative biomedical applications. Nylon 6 materials can be given antibacterial properties, cell adhesion and drug release capabilities by introducing different functional groups, and controllable biocompatibility can be obtained, which not only ensures the safety of the material, but also meets the needs of different applications, helping to improve the application performance of biomaterials in the medical field and overcome the limitations of traditional biomaterials.

[0004] However, traditional biomaterials do not have multiple functionalities, and have poor antibacterial properties and insufficient angiogenesis-inducing ability, which greatly limits their application in the medical field. Therefore, in order to give full play to the excellent performance of nylon 6 materials, it is necessary to comprehensively improve nylon 6 materials so that they have biocompatibility, antibacterial and angiogenesis-promoting properties. The present invention can expand the application scope of Nylon 6 materials to fields including wound dressings, bone repair materials, etc., providing a new solution for biomedical materials. Summary of the invention

[0005] In order to overcome the shortcomings of the prior art, one of the objects of the present invention is to provide a method for preparing an antibacterial and angiogenic nylon 6 material, which has a simple preparation process and can solve the problem of poor stability of nylon 6 material in the fields of wound dressings and bone repair due to insufficient antibacterial ability and angiogenesis ability.

[0006] The second object of the present invention is to provide an antibacterial and angiogenic nylon 6 material obtained according to the above preparation method, wherein the prepared nylon 6 material has both more excellent high-efficiency antibacterial and angiogenic activities.

[0007] One of the purposes of the present invention is achieved by the following technical solution:

[0008] A method for preparing an antibacterial and angiogenic nylon 6 material comprises the following steps:

[0009] (1) 3D printing nylon 6 powder to obtain a 3D printed nylon 6 bracket;

[0010] (2) preparing a dopa compound and angiogenesis-promoting protein into an aqueous solution, then immersing a nylon 6 scaffold material therein, adjusting the pH, heating and stirring, and preparing angiogenesis-promoting nylon 6 scaffold;

[0011] (3) An amino group-containing antibacterial drug and a phosphate group-containing compound are prepared into an aqueous solution, and the nylon 6 stent obtained in step (2) is immersed in the aqueous solution, heated, and then cooled to obtain an antibacterial and angiogenic nylon 6 stent.

[0012] Furthermore, in step (2), the dopa-like compound is at least one of dopamine, epinephrine and norepinephrine.

[0013] Furthermore, the angiogenesis-promoting protein in step (2) is at least one of basic fibroblast growth factor, vascular endothelial growth factor, transforming growth factor β, and platelet-derived growth factor bb.

[0014] Furthermore, in step (3), the amino group-containing antibiotic is at least one of tobramycin, gentamicin, ampicillin, and floxacin, and the phosphate group-containing compound is at least one of guanosine-5'-disodium triphosphate and guanosine-5'-sodium diphosphate.

[0015] Furthermore, in step (2), the concentration of the dopa compound is 1-5 mg / mL, the concentration of the angiogenesis-promoting protein is 10-100 μg / mL, the reaction temperature is 20-30° C., and the reaction time is 12-36 h.

[0016] Furthermore, in step (3), the concentration of the amino-containing antibacterial drug is 5-50 mg / mL, the concentration of the compound containing a phosphate group is 20-100 mg / mL, the heating temperature is 40-60° C., and the time is 1-3 h.

[0017] Furthermore, the preparation method of the 3D printed nylon 6 bracket in step (1) is as follows:

[0018] The caprolactam and the catalyst are heated and stirred, and then anhydrous ethanol is added to continue the reaction under heating and pressurization conditions. The powder is washed and dried to obtain nylon 6 powder, which is loaded into a 3D printer and printed using selective laser sintering technology to obtain a 3D printed nylon 6 bracket.

[0019] Furthermore, the catalyst is at least one of concentrated sulfuric acid, sodium hydroxide and hydrogen peroxide, the mass ratio of the catalyst to caprolactam is 0.5-3:100, the reaction temperature is 240-260° C., and the reaction time is 8-16 hours.

[0020] Furthermore, the mass ratio of caprolactam to anhydrous ethanol is 1:5-1:10, the reaction pressure is 0.5-1MPa, the temperature is 100-120°C, and the time is 4-8h.

[0021] The second object of the present invention is achieved by adopting the following technical solution:

[0022] An antibacterial and angiogenesis-promoting nylon 6 material is prepared by the preparation method.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] 1. The present invention provides a method for preparing an antibacterial and angiogenic nylon 6 material. The surface of the nylon 6 material is modified by a dopa compound to give the nylon 6 material antibacterial properties and surface grafting sites. The dopa compound coats the protein during the polymerization process. The dopa compound has multiple amino groups. The compound containing the phosphate group forms hydrogen bonds with the amino group, and then the antibacterial drug is coated on the surface of the stent, so that the nylon 6 obtains a uniform antibacterial gel coating and angiogenic surface.

[0025] 2. The present invention also provides an antibacterial and angiogenic nylon 6 material prepared by the above-mentioned preparation method. The antibacterial and angiogenic nylon 6 material prepared by the present invention is modified layer by layer to finally make it work as a whole, and synergistically improve the comprehensive performance of the nylon 6 material. The surface of the nylon 6 material obtained by the present invention is uniform, and it has good antibacterial and angiogenic activities. On the one hand, it can be highly effective in antibacterial, help the infection in the early stage of wound healing, and accelerate the recovery of the lesion site; on the other hand, the prepared nylon 6 material has good angiogenic ability, and its biological functionality is far superior to that of the unmodified nylon 6 scaffold, and it has broad application prospects in the field of bone repair and bone regeneration in medicine. DETAILED DESCRIPTION

[0026] The present invention is further described below in conjunction with specific implementation methods. It should be noted that, under the premise of no conflict, the various embodiments or technical features described below can be arbitrarily combined to form a new embodiment.

[0027] Example 1

[0028] A method for preparing an antibacterial and angiogenic nylon 6 material comprises the following steps:

[0029] (1) 100 g of caprolactam powder and 0.5 g of concentrated sulfuric acid were stirred and reacted at 240° C. for 16 h to obtain nylon 6, and then 500 g of anhydrous ethanol was added and the reaction was continued at 100° C. and 0.8 MPa for 8 h. The mixture was washed, filtered, and dried to obtain nylon 6 powder.

[0030] (2) Nylon 6 powder was loaded into a 3D printer and printed using selective laser sintering technology. The printing parameters were: layer height 50 μm, scanning speed 500 mm / s, laser power 2 W, pore size 100 μm. After printing according to the above printing parameters, a porous 3D printed nylon 6 bracket was obtained.

[0031] (3) 100 mg of dopamine and 1 mg of basic fibroblast growth factor (bFGF) were taken respectively and added into 100 mL of deionized water to prepare an aqueous solution of the two. Then, the nylon 6 scaffold material was immersed in the aqueous solution, the pH was adjusted to 9, and the reaction was carried out at 20° C. for 36 h to prepare a nylon 6 scaffold that promotes angiogenesis.

[0032] (4) Weigh 500 mg of tobramycin and 2 g of guanosine-5'-triphosphate disodium salt respectively and add them into 100 mL of deionized water, then immerse the nylon 6 stent with pro-angiogenic properties obtained in step (3) therein, heat at 40° C. for 3 h, and then cool to form an antibacterial gel coating, and then rinse with deionized water to remove the gel not deposited on the stent surface, thereby obtaining an antibacterial and pro-angiogenic nylon 6 stent.

[0033] Example 2

[0034] A method for preparing an antibacterial and angiogenic nylon 6 material comprises the following steps:

[0035] (1) 100 g of caprolactam powder and 3 g of sodium hydroxide were stirred and reacted at 260° C. for 8 h to obtain nylon 6, and then 1000 g of anhydrous ethanol was added and the reaction was continued at 120° C. and 1.0 MPa for 4 h. The mixture was washed, filtered, and dried to obtain nylon 6 powder.

[0036] (2) Nylon 6 powder was loaded into a 3D printer and printed using selective laser sintering technology. The printing parameters were: layer height 100 μm, scanning speed 2000 mm / s, laser power 10 W, and pore size 600 μm. After printing according to the above printing parameters, a porous 3D printed nylon 6 bracket was obtained.

[0037] (3) Take 500 mg of adrenaline and 10 mg of vascular endothelial growth factor (VEGF) respectively, add them to 100 mL of deionized water to prepare an aqueous solution of the two, then soak the nylon 6 scaffold material in it, adjust the pH to 11, and react at 30°C for 12 hours to prepare a nylon 6 scaffold that promotes angiogenesis.

[0038] (4) Weigh 5 g of gentamicin and 10 g of guanosine-5'-diphosphate sodium respectively and add them into 100 mL of deionized water, then immerse the nylon 6 stent with pro-angiogenic properties obtained in step (3) therein, heat at 60° C. for 1 h, and then cool to form an antibacterial gel coating, and rinse with deionized water to remove the gel not deposited on the stent surface, thereby obtaining an antibacterial and pro-angiogenic nylon 6 stent.

[0039] Example 3

[0040] A method for preparing an antibacterial and angiogenic nylon 6 material comprises the following steps:

[0041] (1) 100 g of caprolactam powder and 2 g of hydrogen peroxide were stirred and reacted at 250° C. for 12 h to obtain nylon 6, and then 750 g of anhydrous ethanol was added and the reaction was continued at 110° C. and 0.9 MPa for 6 h. The mixture was washed, filtered, and dried to obtain nylon 6 powder.

[0042] (2) Nylon 6 powder was loaded into a 3D printer and printed using selective laser sintering technology. The printing parameters were: layer height 75 μm, scanning speed 1000 mm / s, laser power 6 W, and pore size 400 μm. After printing according to the above printing parameters, a porous 3D printed nylon 6 bracket was obtained.

[0043] (3) Take 250 mg of norepinephrine and 5 mg of transforming growth factor β (TGF-β) respectively, add them to 100 mL of deionized water to prepare an aqueous solution of the two, then soak the nylon 6 scaffold material in it, adjust the pH to 10, and react at 25°C for 24 hours to prepare a nylon 6 scaffold that promotes angiogenesis.

[0044] (4) Weigh 2.5 g of ampicillin and 6 g of guanosine-5'-diphosphate sodium respectively and add them into 100 mL of deionized water, then immerse the nylon 6 stent with pro-angiogenic properties obtained in step (3) therein, heat at 60° C. for 1 h, and then cool to form an antibacterial gel coating, and rinse with deionized water to remove the gel not deposited on the stent surface, thereby obtaining an antibacterial and pro-angiogenic nylon 6 stent.

[0045] Example 4

[0046] A method for preparing an antibacterial and angiogenic nylon 6 material comprises the following steps:

[0047] (1) 100 g of caprolactam powder, 1.5 g of hydrogen peroxide and 1.5 g of concentrated sulfuric acid were stirred and reacted at 240° C. for 16 h to obtain nylon 6, and then 500 g of anhydrous ethanol was added and the reaction was continued at 100° C. and 0.8 MPa for 16 h. The mixture was washed, filtered and dried to obtain nylon 6 powder.

[0048] (2) Nylon 6 powder was loaded into a 3D printer and printed using selective laser sintering technology. The printing parameters were: layer height 50 μm, scanning speed 2000 mm / s, laser power 10 W, and pore size 600 μm. After printing according to the above printing parameters, a porous 3D printed nylon 6 bracket was obtained.

[0049] (3) 150 mg of dopamine, 150 mg of norepinephrine, and 1 mg of platelet-derived growth factor bb (PDGF-bb) were taken respectively and added to 100 mL of deionized water to prepare an aqueous solution, and then the nylon 6 scaffold material was immersed in the aqueous solution, the pH was adjusted to 9, and the solution was reacted at 25°C for 36 hours to prepare a nylon 6 scaffold that promotes angiogenesis.

[0050] (4) Weigh 2 g of floxacin and 2 g of guanosine-5'-triphosphate disodium respectively and add them into 100 mL of deionized water, then immerse the nylon 6 stent with pro-angiogenic properties obtained in step (3) therein, heat at 50° C. for 2 h, and then cool to form an antibacterial gel coating, and rinse with deionized water to remove the gel not deposited on the stent surface, thereby obtaining an antibacterial and pro-angiogenic nylon 6 stent.

[0051] Example 5

[0052] A method for preparing an antibacterial and angiogenic nylon 6 material comprises the following steps:

[0053] (1) 100 g of caprolactam powder, 1.5 g of hydrogen peroxide and 1.5 g of sodium hydroxide were stirred and reacted at 260° C. for 8 h to obtain nylon 6, and then 1000 g of anhydrous ethanol was added and the reaction was continued at 100° C. and 0.8 MPa for 16 h. The mixture was washed, filtered and dried to obtain nylon 6 powder.

[0054] (2) Nylon 6 powder was loaded into a 3D printer and printed using selective laser sintering technology. The printing parameters were: layer height 100 μm, scanning speed 5000 mm / s, laser power 2 W, pore size 300 μm. After printing according to the above printing parameters, a porous 3D printed nylon 6 bracket was obtained.

[0055] (3) 100 mg of dopamine, 100 mg of norepinephrine, 2.5 mg of basic fibroblast growth factor (bFGF), and 2.5 mg of platelet-derived growth factor bb (PDGF-bb) were taken respectively and added to 100 mL of deionized water to prepare an aqueous solution, and then the nylon 6 scaffold material was immersed in it, the pH was adjusted to 10, and the reaction was carried out at 24°C for 36 hours to prepare a nylon 6 scaffold that promotes angiogenesis.

[0056] (4) Weigh 1 g of tobramycin, 1 g of floxacin, 2 g of guanosine-5'-diphosphate sodium and 2 g of guanosine-5'-triphosphate disodium respectively and add them into 100 mL of deionized water, then immerse the nylon 6 stent with pro-angiogenic properties obtained in step (3) therein, heat at 60° C. for 1 h, and then cool to form an antibacterial gel coating, and rinse with deionized water to remove the gel not deposited on the stent surface, thereby obtaining an antibacterial and pro-angiogenic nylon 6 stent.

[0057] Comparative Example 1

[0058] Comparative Example 1 provides a method for preparing an antibacterial and angiogenic nylon 6 material. The difference from Example 1 is that the operation in step (4) is omitted, and the rest is the same as Example 1.

[0059] Comparative Example 2

[0060] Comparative Example 2 provides a method for preparing an antibacterial and angiogenic nylon 6 material, which differs from Example 1 in that the operation in step (3) is omitted, and the rest is the same as Example 1.

[0061] Comparative Example 3

[0062] Comparative Example 3 provides a method for preparing an antibacterial and pro-angiogenic nylon 6 material, which differs from Example 1 in that the operations in steps (3) and (4) are omitted, and the rest is the same as Example 1.

[0063] Comparative Example 4

[0064] Comparative Example 4 provides a method for preparing an antibacterial and pro-angiogenic nylon 6 material. The difference from Example 1 is that the nylon 6 stent obtained in the above step (2) is first subjected to the operation of step (4) to obtain a nylon 6 stent material with an antibacterial coating, and then the operation of step (3) is performed.

[0065] Comparative Example 5

[0066] Comparative Example 5 provides a method for preparing an antibacterial and angiogenic nylon 6 material, which differs from Example 1 in that tobramycin is replaced with silver chloride, and the rest is the same as Example 1.

[0067] Comparative Example 6

[0068] Comparative Example 6 provides a method for preparing an antibacterial and angiogenic nylon 6 material, which differs from Example 1 in that step (3) is as follows: dopamine is omitted, 1 mg of basic fibroblast growth factor (bFGF) is taken, added to 100 mL of deionized water to prepare a bFGF aqueous solution, and then the nylon 6 scaffold material prepared in step (2) is immersed therein, the pH is adjusted to 9, and the reaction is carried out at 20° C. for 36 hours to obtain a nylon 6 scaffold material that promotes angiogenesis. The subsequent operations are the same as those in Example 1.

[0069] Comparative Example 7

[0070] Comparative Example 7 provides a method for preparing an antibacterial and pro-angiogenic nylon 6 material, which differs from Example 1 in that step (4) is as follows: omitting guanosine-5'-triphosphate disodium, directly adding 500 mg of tobramycin to 100 mL of deionized water, and then immersing the pro-angiogenic nylon 6 scaffold obtained in step (3) therein, heating at 40°C for 3 h and cooling to form an antibacterial gel coating, and then rinsing with deionized water to remove the gel not deposited on the surface of the scaffold.

[0071] In order to verify whether the nylon 6 stent material with antibacterial and angiogenesis-promoting functions of the present invention achieves the expected effect, antibacterial experiments and in vitro scratch healing experiments were performed on the stent materials prepared in Examples 1-5 and Comparative Examples 1-7.

[0072] Test Example 1

[0073] 1. Antibacterial test

[0074] 1 g of nylon 6 scaffolds prepared in different examples and comparative examples were placed in sterile conical flasks, and 50 mL of 0.03 mol / L phosphate buffer and 5 mL of 10 8 cfu / mL of Staphylococcus aureus or Escherichia coli liquid was incubated at 37°C for 12h and 24h after the liquid had infiltrated the culture medium, and the colonies were counted.

[0075] The antibacterial rate calculation formula is: X = (AB) / A × 100%, where X is the antibacterial rate, A is the average colony count before the test sample is shaken, and B is the average colony count after the test sample is shaken. The antibacterial rate results of Staphylococcus aureus and Escherichia coli of nylon 6 scaffold materials prepared in different embodiments and comparative examples are shown in Tables 1 and 2.

[0076] Table 1

[0077]

[0078]

[0079] Table 2

[0080] 12h / ％ 24h / ％ Example 1 81.29 92.54 Example 2 84.78 93.99 Example 3 84.29 95.74 Example 4 83.13 93.61 Example 5 83.71 94.58 Comparative Example 1 12.22 22.21 Comparative Example 2 80.61 91.37 Comparative Example 3 25.90 30.75 Comparative Example 4 23.65 31.67 Comparative Example 5 0 0 Comparative Example 6 20.19 24.37 Comparative Example 7 0 0

[0081] From the results in Table 1 and Table 2, it can be seen that the nylon 6 stent material with antibacterial and angiogenesis-promoting functions prepared by the present invention has extremely strong antibacterial properties. Comparative Examples 1 and 3 are not modified with antibacterial gel coatings on their surfaces, and the antibacterial rates are much lower than those of the modified Examples 1 to 5. Therefore, the modification of the antibacterial gel coating makes the prepared nylon 6 stent have high antibacterial properties.

[0082] Comparative Example 4 is to first modify the nylon 6 stent with an antibacterial coating, and then modify it with dopamine and surface factor bFGF. After 24 hours, its antibacterial rates against Staphylococcus aureus and Escherichia coli are lower than those in Example 1. Comparative Example 5 replaces tobramycin with silver chloride, and Comparative Example 6 replaces tobramycin with silver chloride without soaking treatment with dopamine and bFGF. Comparative Example 7 omits disodium guanosine-5'-triphosphate, and prepares nylon 6 stents respectively. It can be seen from the experimental results of Table 1 and Table 2 that the antibacterial rates of the three after 24 hours of culture are not as good as those of the example group, which further illustrates that the amino-containing antibacterial drug and the phosphate-containing compound form a more stable antibacterial coating on the surface of nylon 6, which has a high antibacterial effect.

[0083] Compared with Comparative Examples 1-7, the antibacterial rate of the nylon 6 stent prepared in Example 1-5 was maintained above 90% after 24 hours, which has a more excellent antibacterial rate. This shows that the introduction of the above-mentioned dopa-like compounds and angiogenesis-promoting proteins, as well as the dual effects of the antibacterial gel coating, help to effectively improve the antibacterial effect of the nylon 6 material.

[0084] 2. In vitro scratch healing assay

[0085] (1) HUVECs cells were cultured at 1×10 4 The cells were seeded into 6-well plates at a density of 10 cells / well and incubated overnight until the cells reached 90% confluence;

[0086] (2) Use a sterile 200 μL pipette tip to scratch the bottom of the well plate to form a cell-free strip in the center of the HUVECs monolayer;

[0087] (3) Discard the original culture medium and wash with PBS solution to remove non-adherent cells and cell debris. Place the sterilized antibacterial and angiogenic nylon 6 scaffold on the Transwell, and place the Transwell on the culture plate. After incubation in the cell culture incubator for 12 hours and 24 hours, take pictures of the scratches under a microscope;

[0088] (4) Then, the scratch area was quantitatively analyzed using Image J software, and the scratch healing rate was calculated using formula (2-1):

[0089]

[0090] Wherein: X is the scratch area; At is the scratch area at different time points; A0 is the scratch area at 0h. The scratch healing rates of nylon 6 stent materials of different embodiments are shown in Table 3.

[0091] Table 3

[0092] 12h / ％ 24h / ％ Example 1 65.42 21.54 Example 2 66.37 23.19 Example 3 59.64 25.38 Example 4 62.13 23.67 Example 5 58.37 24.79 Comparative Example 1 62.67 26.47 Comparative Example 2 85.46 54.87 Comparative Example 3 87.63 55.67 Comparative Example 4 77.39 38.73 Comparative Example 5 67.34 33.76 Comparative Example 6 81.37 53.34 Comparative Example 7 62.37 29.21

[0093] As can be seen from the above table, the nylon 6 scaffold material with antibacterial and angiogenic functions prepared by the present invention has extremely strong angiogenic performance. Comparative Examples 2 and 3 are nylon 6 scaffolds prepared without modification with dopamine compounds and angiogenic factors, and their scratch healing rates are much higher than those of Examples 1-5 and Comparative Example 1, and their angiogenic ability is extremely weak, indicating that the treatment with dopamine and bFGF makes the nylon 6 scaffold have good angiogenic performance.

[0094] In Examples 1-5 and Comparative Example 1, scratches can heal quickly due to the loading of proteins that promote angiogenesis. After 24 hours of experiment, it was found that the scratch healing rate of Experimental Example 1 was lower than that of Comparative Example 1, which was 21.54%, indicating that its ability to promote vascular healing was better than that of Comparative Example 1, and the antibacterial coating on the surface of the antibacterial nylon 6 stent could effectively promote its angiogenesis ability. This shows that, under the premise that the nylon 6 stent has the ability to promote angiogenesis, the superimposed modification of the antibacterial gel coating on its surface can not only make the prepared nylon 6 stent material highly antibacterial, but also accelerate the growth of blood vessels and shorten the wound healing time.

[0095] In summary, the antibacterial and pro-angiogenic nylon 6 stent provided by the present application improves the surface activity of the nylon 6 material by modifying the surface of the nylon 6 stent, fixes the protein with pro-angiogenic function on its surface, and then modifies the antibacterial gel coating on its surface. Specifically, the antibacterial drug containing amino group forms hydrogen bond interaction with the compound containing phosphate group to form a uniform antibacterial gel coating on the surface of the pro-angiogenic nylon 6 stent, and can synergistically act with dopa compounds and pro-angiogenic proteins to comprehensively improve the biocompatibility of the nylon 6 stent, so that it has more excellent antibacterial and pro-angiogenic activity.

[0096] The above-mentioned embodiments are only preferred embodiments of the present invention and cannot be used to limit the scope of protection of the present invention. Any non-substantial changes and substitutions made by technicians in this field on the basis of the present invention shall fall within the scope of protection required by the present invention.

Claims

1. A method for preparing an antibacterial and angiogenic nylon 6 material, characterized in that: The steps include: (1) 3D printing nylon 6 powder to obtain a 3D printed nylon 6 bracket; (2) preparing a dopa compound and angiogenesis-promoting protein into an aqueous solution, then immersing a nylon 6 scaffold material therein, adjusting the pH, heating and stirring, and preparing angiogenesis-promoting nylon 6 scaffold; (3) preparing an aqueous solution of an antibacterial drug containing an amino group and a compound containing a phosphate group, and then immersing the angiogenesis-promoting nylon 6 stent obtained in step (2) therein, heating, and then cooling, to obtain an antibacterial and angiogenesis-promoting nylon 6 stent; The compound containing a phosphate group is at least one of guanosine-5'-disodium triphosphate and guanosine-5'-sodium diphosphate.

2. The method for preparing the antibacterial and angiogenic nylon 6 material according to claim 1, characterized in that: In step (2), the dopa compound is at least one of dopamine, epinephrine and norepinephrine.

3. The method for preparing the antibacterial and angiogenic nylon 6 material according to claim 1, characterized in that: The angiogenesis-promoting protein in step (2) is at least one of basic fibroblast growth factor, vascular endothelial growth factor, transforming growth factor β, and platelet-derived growth factor bb.

4. The method for preparing the antibacterial and angiogenic nylon 6 material according to claim 1, characterized in that: In step (3), the amino-containing antibacterial drug is at least one of tobramycin, gentamicin and ampicillin.

5. The method for preparing the antibacterial and angiogenic nylon 6 material according to claim 1, characterized in that: In step (2), the concentration of the dopa compound is 1-5 mg / mL, the concentration of the angiogenesis-promoting protein is 10-100 μg / mL, the reaction temperature is 20-30° C., and the reaction time is 12-36 h.

6. The method for preparing the antibacterial and angiogenic nylon 6 material according to claim 1, characterized in that: In step (3), the concentration of the amino-containing antibacterial drug is 5-50 mg / mL, the concentration of the compound containing a phosphate group is 20-100 mg / mL, the heating temperature is 40-60° C., and the time is 1-3 h.

7. The method for preparing the antibacterial and angiogenic nylon 6 material according to claim 1, characterized in that: The preparation method of the 3D printed nylon 6 bracket in step (1) is as follows: The caprolactam and the catalyst are heated and stirred, and then anhydrous ethanol is added to continue the reaction under heating and pressurization conditions. The powder is washed and dried to obtain nylon 6 powder, which is loaded into a 3D printer and printed using selective laser sintering technology to obtain a 3D printed nylon 6 bracket.

8. The method for preparing the antibacterial and angiogenic nylon 6 material according to claim 7, characterized in that: The catalyst is at least one of concentrated sulfuric acid, sodium hydroxide and hydrogen peroxide, the mass ratio of the catalyst to caprolactam is 0.5-3:100, the reaction temperature is 240-260° C., and the reaction time is 8-16 hours.

9. The method for preparing the antibacterial and angiogenic nylon 6 material according to claim 7, characterized in that: The mass ratio of caprolactam to anhydrous ethanol is 1:5-1:10, the reaction pressure is 0.5-1MPa, the temperature is 100-120°C, and the time is 4-8h.

10. An antibacterial and angiogenic nylon 6 material, characterized in that: Prepared by the method according to any one of claims 1 to 9.

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