A miticidal composition containing ethyl mepiperphenidol and flubenzimine

Abstract

The present invention provides a miticidal composition containing ethyl mepiperphenidol and pyridaben. Its first active ingredient is ethyl mepiperphenidol; the second active ingredient is pyridaben. The weight ratio of the first active ingredient to the second active ingredient is 8:1 - 1:2. The composition of the present invention exhibits obvious synergistic effects within a certain ratio, reduces the amount of pesticide used, cuts costs, and delays the generation of pest resistance.

Description

Technical Field

[0001] The present invention belongs to the field of acaricides, and particularly relates to an acaricidal composition containing ethyl methoprene and fluacrypyrim. Background Art

[0002] Ethyl methoprene is a spirotetronic acid insecticide developed by Syngenta. Its mechanism of action is a lipid biosynthesis inhibitor. By inhibiting the activity of ACCase in the process of fat synthesis in pests, it disrupts lipid synthesis, thereby blocking the normal energy metabolism of pests and ultimately causing the death of pests. It can control pests such as aphids, whiteflies, psyllids, armored scales, soft scales, spider mites, rust mites, red spider mites, peach aphids, tobacco whiteflies, cotton aphids, etc.

[0003] Fluacrypyrim is a new acaricide developed by Shenyang Research Institute of Chemical Industry. Fluacrypyrim belongs to an endosystemic acaricide, mainly acting by contact, and is effective against egg mites, young and nymph mites, and adult mites. It can control various common agricultural acarids such as citrus red spider mites, apple red spider mites, two-spotted spider mites, and carmine spider mites.

[0004] Agricultural acarids are one of the important pests in agricultural production. They mainly damage the leaves, tender stems, leaf sheaths, flower buds, fruits, tubers, and rhizomes of crops, sucking the plant sap, making the damaged parts lose green color, wither, or deform. At present, for controlling acarids, chemical control is still the most common and effective means. However, due to the long-term continuous application of a single chemical insecticide, many acarids have developed resistance to acaricides, and the development speed of new compound agents is far slower than the speed of the emergence of pest resistance. Reasonable compounding of agents can improve the efficacy of the agents and reduce the generation of acarid resistance. In the prior art, the technical solutions of compositions containing ethyl methoprene and fluacrypyrim have not been specifically disclosed. Summary of the Invention

[0005] The purpose of the present invention is to provide an acaricidal composition with good acaricidal effect and low application cost, wherein the active ingredients of the composition include ethyl methoprene and fluacrypyrim.

[0006] To achieve the above purpose, the technical solution of the present invention is solved as follows:

[0007] An acaricidal composition containing ethyl methoprene and fluacrypyrim provided by the present invention, its first active ingredient: ethyl methoprene; second active ingredient: fluacrypyrim. The weight ratio of the first active ingredient to the second active ingredient is 8:1 - 1:2, preferably 2:1.

[0008] An acaricidal composition containing ethyl methoprene and fluacrypyrim provided by the present invention, calculated by weight percentage, the composition accounts for 4% - 60% of the total content of the preparation.

[0009] A miticide composition containing ethyl methoxypiperidine carboxylate and flubenzimine provided by the present invention can be formulated into suspension concentrates, dispersible oil suspension concentrates and water dispersible granules, preferably suspension concentrates.

[0010] A miticide composition containing ethyl methoxypiperidine carboxylate and flubenzimine provided by the present invention is suitable for controlling agricultural mites, including Panonychus citri, Panonychus ulmi, Tetranychus urticae, Tetranychus cinnabarinus, Tetranychus viennensis, Tetranychus turkestani, etc.

[0011] The application method of the composition is spraying.

[0012] The dosage of the active ingredients of the composition is 75-135 g / ha.

[0013] The amount of water added to the composition is 1200-1800 kg / ha.

[0014] The composition is sprayed once at the early peak stage of mite infestation, and is used at most once per crop season.

[0015] The beneficial effects are as follows:

[0016] The composition of the present invention shows obvious synergistic effects within a certain ratio, reduces the amount of pesticides used, reduces costs, and delays the generation of pest resistance. Specific embodiments

[0017] Indoor bioassay

[0018] The determination method refers to "NY / T 1154.12-2008 Guidelines for Pesticide Bioassay in the Laboratory - Part 12: Slide Immersion Method for Tetranychidae".

[0019] Test object: Panonychus citri

[0020] Test agents: ethyl methoxypiperidine carboxylate technical, flubenzimine technical

[0021] First, cut double-sided tape into pieces about 2 cm long and stick it to one end of a glass slide. Use a fine brush to select female adult mites that are active and of the same size from indoor breeding, and stick their backs to the double-sided

[0022] tape. Pay attention not to stick the mite legs, antennae and mouthparts. Place 30 mites on each slide in 3 rows, and then put them in a porcelain dish lined with wet filter paper and culture them under the condition of 25±1°C. After 2 h, examine them under a microscope, remove dead and injured individuals, and make up 30 mites on each slide.

[0023] Dissolve ethyl methoxypiperidine carboxylate technical and flubenzimine technical with acetone to prepare a mother liquor, and then dilute it into 5 series of liquid medicines with different mass concentrations with 0.1% Tween 80 aqueous solution according to a certain ratio.

[0024] Place the glass slide in the prepared liquid medicine, gently shake it for 5 s, then take it out, blot the excess liquid medicine with absorbent paper, place it in a porcelain dish padded with wet filter paper, cover it with a plastic film with good light transmittance, repeat each treatment 4 times, and set the treatment without the medicine as the blank control. Place the porcelain dish with the treated test insects in an environment with a temperature of 25 ± 1 °C and a photoperiod of L:D = (16, 8) h for breeding and observation. Check the death situation of the test insects 48 h after the treatment, and record the total number of insects and the number of dead insects respectively.

[0025] Perform regression analysis on the logarithmic values of the concentrations of each medicine and the corresponding mortality probability values, and calculate the LC of each medicine treatment 50 . Evaluate the interaction of the mixed agents according to the Wadley method. Among them, if SR = 0.5 - 1.5, the two agents have an additive effect when compounded; if SR ≤ 0.5, it is an antagonistic effect; if SR ≥ 1.5, it is a synergistic effect.

[0026] LC 50 (theoretical value) = (a + b) / (a / LC 50 a + b / LC 50 b)

[0027] SR = LC 50 (theoretical value) / LC 50 (actual value)

[0028] The test results are shown in Table 1.

[0029] Table 1 Indoor combined toxicity determination results of the acaricidal composition against Panonychus citri

[0030]

[0031]

[0032] As can be seen from Table 1, after compounding ethyl methoprene and flubenzolate according to the test ratio, they show two different combined effects of addition and synergism against Panonychus citri. When the mass ratio of ethyl methoprene to flubenzolate is 8:1 - 1:2, the compounded medicine shows a synergistic effect against Panonychus citri. Especially when the mass ratio of ethyl methoprene to flubenzolate is 2:1, the SR value is 4.71, showing a significant synergistic effect.

[0033] Formulation Example 24% Ethyl Methoprene · Flubenzolate Suspension Concentrate

[0034] Weigh 160 g of ethyl methopiperidine carboxylate, 80 g of flufenerim, 60 g of lignosulfonate, 50 g of sodium dodecyl sulfate, 4 g of silicone, 20 g of magnesium aluminum silicate, 40 g of ethylene glycol, 5 g of sodium benzoate, and add deionized water to 1000 g by weight. The above raw materials are mixed, sheared and dispersed at high speed for 30 min, and then ground with a sand mill until the particle size D90 is less than 5 μm to obtain a 24% ethyl methopiperidine carboxylate·flufenerim suspension concentrate.

[0035] Comparative Example 1: 20% ethyl methopiperidine carboxylate suspension concentrate

[0036] Weigh 200 g of ethyl methopiperidine carboxylate, 60 g of lignosulfonate, 50 g of sodium dodecyl sulfate, 4 g of silicone, 20 g of magnesium aluminum silicate, 40 g of ethylene glycol, 5 g of sodium benzoate, and add deionized water to 1000 g by weight. The above raw materials are mixed, sheared and dispersed at high speed for 30 min, and then ground with a sand mill until the particle size D90 is less than 5 μm to obtain a 20% ethyl methopiperidine carboxylate suspension concentrate.

[0037] Comparative Example 2: 15% flufenerim suspension concentrate

[0038] Weigh 150 g of flufenerim, 60 g of lignosulfonate, 50 g of sodium dodecyl sulfate, 4 g of silicone, 20 g of magnesium aluminum silicate, 40 g of ethylene glycol, 5 g of sodium benzoate, and add deionized water to 1000 g by weight. The above raw materials are mixed, sheared and dispersed at high speed for 30 min, and then ground with a sand mill until the particle size D90 is less than 5 μm to obtain a 15% flufenerim suspension concentrate.

[0039] Field efficacy test

[0040] The applicant conducted a field efficacy test on the control of Panonychus citri by the formulation example in Ledong Li Autonomous County, Hainan Province in 2023.

[0041] Test crops and targets: citrus, Panonychus citri.

[0042] Test method:

[0043] Drug setting: The test drug is Formulation Example 1, with 3 treatment doses set, a single-agent control drug and a water control set, each treatment has 4 replicates, arranged in a randomized block design, and each plot has 2 citrus trees.

[0044] Application time, frequency, dosage and water consumption: Apply the drug at the beginning of the peak period of Panonychus citri, spray once, the dosage of the active ingredient of the test drug is 75, 105, 135 g / ha, and the water consumption is 1500 kg / ha.

[0045] Adopt the fixed-point investigation method: Investigate 2 citrus trees in each plot. Mark five points, namely east, south, west, north, and middle, on each tree. Select 1 shoot at each point and investigate 5 leaves on each shoot. A total of 30 leaves are investigated in each plot. Conduct investigations once before applying the drug and on the 3rd, 7th, and 15th days after applying the drug. Check and record the number of active mites on the leaves, calculate the mite population reduction rate and the control effect of the treated agent. The test results are shown in Table 2 below.

[0046] Table 2 Field efficacy test results of the compound preparation against Panonychus citri

[0047]

[0048] It can be seen from Table 2 that the compound preparation of ethyl methoprene and flubenzimine has good quick-acting and long-lasting effects against Panonychus citri when investigated on the 3rd, 7th, and 15th days after application. When the dosage of the active ingredients of the compound preparation is 75 g / ha, 105 g / ha, and 135 g / ha, the control effects against Panonychus citri after 15 days are 83.0%, 87.2%, and 92.1% respectively, all higher than the control effects of 75.4% and 79.3% of the two single agents at the dosage of 105 g / ha of the comparative active ingredient. This further verifies that the compounding of ethyl methoprene and flubenzimine has a certain synergistic effect.

[0049] In summary, through indoor toxicity determination and field control efficacy tests, the acaricidal composition prepared by compounding ethyl methoprene and flubenzimine described in the present invention shows good control effects against harmful mites in agriculture, is superior to single agents in delaying the generation of drug resistance and prolonging the drug persistence, and no phytotoxicity to crops is found in the test, indicating that the compound preparation has significant effects and is worthy of promotion in agricultural production practice.

Claims

1. A miticidal composition containing ethyl mepiperphenidol and flubenzimine, characterized in that , The first active ingredient: ethyl mepiperphenidol; the second active ingredient: flubenzimine; the mass ratio of the first active ingredient to the second active ingredient is 8:1 to 1:

2.

2. The acaricidal composition according to claim 1, characterized in that: The mass ratio of the first active ingredient to the second active ingredient is 2:

1.

3. The acaricidal composition according to any one of claims 1-2, characterized in that: The acaricidal composition accounts for 4% - 60% of the total content of the preparation.

4. The acaricidal composition according to any one of claims 1-2, characterized in that: The dosage form of the composition is suspension concentrate, dispersible oil suspension concentrate and water dispersible granule.

5. The acaricidal composition according to claim 4, characterized in that: The dosage form of the composition is suspension concentrate.

6. Application of a preparation for controlling agricultural pest mites, characterized in that: The preparation is made from the acaricidal composition according to any one of claims 1 - 5.

7. The application according to claim 6, wherein: It is used for controlling Panonychus citri, Panonychus ulmi, Tetranychus urticae, Tetranychus cinnabarinus, Tetranychus viennensis and Tetranychus turkestani.

8. The application according to claim 6, characterized in that: The application method is spraying.

9. The application according to claim 6, wherein: The dosage of the active ingredient is 75 - 135 g / ha.

10. The application according to claim 6, wherein: The amount of water added during use is 1200 - 1800 kg / ha.

11. The application according to claim 6, wherein: Spray the medicine once at the initial peak stage of harmful mites, and use it at most once per crop season.

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