High-throughput, high-speed cooling and transfer system

Abstract

The present invention relates to a high-throughput, high-speed cooling and transport system, comprising a sealed experimental chamber, wherein the experimental chamber is provided with an exhaust port connected to the outside, wherein a low-temperature transport box and a tubular jet generator are provided in the experimental chamber, wherein the low-temperature transport box is driven by an automatic lifting device to move up and down in the experimental chamber to achieve connection or disconnection between the low-temperature transport box and the tubular jet generator; wherein the low-temperature transport box comprises a square box body and a cover body matched with the box body, wherein a card-type sample carrier is provided in the box body. As can be seen from the above technical solution, the present invention can load more samples for high-speed jet impact cooling by optimizing and changing the overall structure of the jet impact method, and can continuously maintain a low-temperature environment for biological materials and conveniently transport them, thereby effectively improving the problems of the traditional jet impact cooling method, such as a small amount of sample carriers, easy temperature rise during the transport process causing cell damage, and possible biological contamination.

Description

Technical Field

[0001] The present invention relates to a high-throughput, high-speed cooling and transport system, which is used for high-throughput low-temperature storage and transfer of biological materials. Background Art

[0002] Vitrification is an important method in biological cryopreservation and has been applied to the long-term preservation of germ cells, stem cells, rare plant seeds, etc. Its technical difficulty lies in the high-speed cooling and reheating of the preserved object. Jet impingement heat exchange refers to the high-speed impact of a working fluid (such as liquid nitrogen, etc.) on the surface of the cooled object for heat exchange. Since the jet boundary layer is extremely thin, jet impingement has a very high heat exchange efficiency and is a key technology for achieving high-speed cooling and reheating of large-volume samples. Due to problems with the sample loading structure and the clamping structure, the current ultra-high-speed jet impingement cooling method can only perform jet impingement cooling on one sample carrier at a time, and the cooling throughput is extremely limited. At the same time, the existing jet impingement cooling system lacks the necessary insulation conditions. After the jet impingement is completed, the sample carrier is directly exposed to the air. During the process of transporting the sample carrier to the liquid nitrogen storage tank, the internal sample temperature rises rapidly, and the sample is prone to recrystallization, devitrification, and other phenomena, resulting in failure of vitrification preservation. Summary of the Invention

[0003] The purpose of the present invention is to provide a high-throughput and high-speed cooling and transport system, which can overcome the problems of the existing jet impact cooling method such as low processing throughput and difficulty in transport after cooling.

[0004] To achieve the above-mentioned object, the present invention adopts the following technical solution: comprising a sealed experimental chamber, the experimental chamber being provided with an exhaust port communicating with the outside, a low-temperature transport box and a tubular jet generator being arranged in the experimental chamber, the low-temperature transport box being driven up and down in the experimental chamber by an automatic lifting device to achieve connection or disconnection between the low-temperature transport box and the tubular jet generator;

[0005] The low-temperature transport box includes a square box body and a cover body that matches the box body. A card-type sample carrier is provided in the box body. The card-type sample carrier is arranged along the width direction of the box body and has a gap with the bottom of the box body. The card-type sample carrier is fixed in the box body by a slot on the inner wall of the box body. The cover body is composed of an exhaust top cover arranged above the card-type sample carrier and a first top cover and a second top cover symmetrically arranged on both sides of the card-type sample carrier. The exhaust top cover includes a first exhaust cover body and a second exhaust cover body that are symmetrically arranged and respectively connected to the box body by a torsion spring hinge. The first top cover and the second top cover are respectively provided with a working medium action hole.

[0006] The tubular jet generator includes a working fluid inlet connector, a diverter row connected to the working fluid inlet connector, and a first jet tube and a second jet tube connected to the diverter row. The diverter row is fixed to the top surface of the experimental chamber and is provided with a hole for the working fluid inlet connector to pass through. The working fluid inlet connector is connected to a liquid nitrogen tank outside the experimental chamber via a pipe. The first jet tube and the second jet tube are provided with jet holes arranged in an array. The first jet tube and the second jet tube are respectively inserted into the working fluid action holes on the first and second top covers, and the jet holes of the first jet tube and the second jet tube are aligned with the two surfaces of the card-type sample carrier.

[0007] The automatic lifting device includes a screw transmission mechanism placed outside the experimental chamber and a workbench placed inside the experimental chamber. The workbench is used to place the low-temperature transfer box. The bottom of the workbench is provided with a transmission sheet metal connected to the slider in the screw transmission mechanism. The screw transmission mechanism is driven by a stepper motor.

[0008] The card-type sample carrier is a hollow structure, including a symmetrically arranged first thermal conductive sheet, a second thermal conductive sheet, and an intermediate layer arranged between the first thermal conductive sheet and the second thermal conductive sheet. The intermediate layer uses a flexible thermal conductive material to etch a space or a flow channel frame. The four corners of the outer surfaces of the first thermal conductive sheet and the second thermal conductive sheet are respectively embedded with attraction magnets. The attraction magnets fix the first thermal conductive sheet, the second thermal conductive sheet and the intermediate layer through strong magnetic action to form a hollow structure. The hollow structure is used to carry hydrogel-encapsulated biological samples.

[0009] The first heat conducting plate and the second heat conducting plate are made of high heat conducting material, and the outer surfaces of the first heat conducting plate and the second heat conducting plate are etched with bionic micro-channels for enhancing the heat exchange effect.

[0010] The card slots are a first slot and a second slot symmetrically arranged on the inner wall of the box body. The card slots can fix one or more card-type sample carriers along their height direction.

[0011] The first jet tube and the second jet tube are rigid tubes, and the ends of the first jet tube and the second jet tube are sealed. The aperture of the jet hole is 0.2 to 2 mm. After the liquid nitrogen enters the diverter row through the working medium inlet joint, it flows evenly into the first jet tube and the second jet tube, and then evenly impacts the outer surface of the cassette sample carrier through the jet hole.

[0012] The workbench is provided with a positioning frame that cooperates with the low-temperature transfer box, and the cavity wall of the experimental cavity is provided with a limit groove that cooperates with the transmission sheet metal. The limit groove is opened in the vertical direction, and the stepper motor drives the transmission sheet metal to move up and down along the direction specified by the limit groove through the slider, thereby realizing the lifting of the workbench.

[0013] A sealing isolation device is also provided in the experimental chamber. The sealing isolation device is arranged at the corresponding position of the limit groove and is used to seal and isolate the inside and outside of the experimental chamber. The sealing isolation device includes an isolation frame fixed on the inner wall of the experimental chamber and an accordion sealing strip arranged in the isolation frame. The accordion sealing strip can expand and contract up and down as the transmission sheet metal moves up and down to form a seal.

[0014] The transmission sheet metal, the limiting groove and the sealing isolation device are each provided with two groups, and the screw in the screw transmission mechanism is a double screw.

[0015] The experimental chamber is also provided with a first pressing block and a second pressing block, the positions of the first pressing block and the second pressing block respectively coincide with the positions of the first top cover and the second top cover, and the first pressing block and the second pressing block are respectively connected to the top surface of the experimental chamber through spring pins.

[0016] The low-temperature transport box is made of heat-insulating material.

[0017] The beneficial effects of the patent of the present invention are: 1) The jet tube in the tubular jet generator of the present invention can be quickly and conveniently aligned with the card-type sample carrier fixed in the low-temperature transport box without the need for other clamping structures; the structural design of the low-temperature transport box makes it possible to no longer limit the number of card-type sample carriers that can be cooled at ultra-high speed each time, and the sample throughput of a single high-speed jet impact cooling can be increased by inserting multiple card-type sample carriers or increasing the number of card slots and the number of jet tubes.

[0018] 2) The exhaust device and the torsion spring hinge of the low-temperature transfer box of the present invention can cooperate to discharge excess gas during the high-speed jet impact cooling period, and after the jet impact is completed, the exhaust top cover is closed to use the remaining liquid nitrogen to continuously evaporate the low-temperature gas. The heated gas will escape through smaller pores such as the working fluid action hole, thereby continuously ensuring the low-temperature environment inside the box, facilitating overall transportation and delivery, avoiding damage to cells caused by devitrification due to sudden temperature changes during transportation, and pollution problems caused by multiple transportation operations. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a schematic diagram of the structure of the present invention after removing the experimental chamber door;

[0020] Figure 2 This is a schematic diagram of the structure of the experimental chamber of the present invention after removing the hatch;

[0021] Figure 3 This is a schematic diagram of the structure of the low-temperature transport box of the present invention Figure 1 ;

[0022] Figure 4 This is a schematic diagram of the structure of the low-temperature transport box of the present invention Figure 2 ;

[0023] Figure 5 1 is a schematic structural diagram of a tubular jet generator according to the present invention;

[0024] Figure 6 This is a schematic diagram of the structure of the card-type sample carrier of the present invention. Figure 1 ;

[0025] Figure 7 yes Figure 6 Schematic diagram of the decomposition structure;

[0026] Figure 8 This is a schematic diagram of the structure of the card-type sample carrier of the present invention. Figure 2 ;

[0027] Figure 9 This is a schematic diagram of the structure of the automatic lifting device of the present invention Figure 1 ;

[0028] Figure 10 This is a schematic diagram of the structure of the automatic lifting device of the present invention Figure 2 ;

[0029] Figure 11 This is a schematic diagram of the structure of the low-temperature transport box, the tubular jet generator and the automatic lifting device of the present invention;

[0030] Figure 12 This is a schematic diagram of the structure of the cooperation between the experimental chamber and the automatic lifting device of the present invention;

[0031] Figure 13 This is the working state of the present invention when jetting Figure 1 ;

[0032] Figure 14 This is the working state of the present invention when jetting Figure 2 ;

[0033] Figure 15 This is a schematic diagram of the present invention when the exhaust cover is opened during jetting;

[0034] Figure 16 Schematic diagram of the structure of the low-temperature transport box according to the second embodiment of the present invention;

[0035] Figure 17 Schematic diagram of a tubular jet generator according to a second embodiment of the present invention;

[0036] Figure 18 This is a line graph of cooling test data according to the first embodiment of the present invention.

[0037] 1 , a first pressure block 12 , a second pressure block 13 , a spring pin 14 , an exhaust port 2 , a cryogenic transfer box 3 , a box body 31 , a first channel 311 , a second channel 312 , a first top cover 321 , a second top cover 322 , a first exhaust cover 323 , a second exhaust cover 324 , a torsion spring hinge 33 , a working fluid action hole 34 , a tubular jet generator 4 , a working fluid inlet joint 41 , a diverter row 42 , a first jet tube 43 , a second jet tube 44 , a jet hole 45 , a card-type sample carrier 5 , a first heat conducting plate 51 , a second heat conducting plate 52 , an intermediate layer 53 , an attraction magnet 54 , a bionic microfluidic channel 55 , an automatic lifting device 6 , a screw transmission mechanism 61 , a workbench 62 , a positioning frame 621 , a transmission sheet metal 63 , a stepping motor 64 , an isolation frame 71 , an accordion sealing strip 72 , and a liquid nitrogen tank 10 . DETAILED DESCRIPTION

[0038] The present invention will be further described below with reference to the accompanying drawings:

[0039] like Figure 1 、 Figure 11 The high-throughput, high-speed cooling and transfer system shown includes a sealed experimental chamber 1, which is provided with an exhaust port 2 connected to the outside, and a low-temperature transfer box 3 and a tubular jet generator 4 are provided in the experimental chamber 1. The low-temperature transfer box 3 is driven by an automatic lifting device 6 to move up and down in the experimental chamber 1 to achieve connection or disconnection between the low-temperature transfer box 3 and the tubular jet generator 4.

[0040] Further, such as Figure 3 、 Figure 4 As shown, the cryogenic transport box 3 is made of a thermally insulating material. In this embodiment, the cryogenic transport box 3 is made of EPS foam. The cryogenic transport box 3 includes a square box body 31 and a cover that cooperates with the box body 31. A card-type sample carrier 5 is disposed within the box body 31. The card-type sample carrier 5 is arranged along the width of the box body and has a gap with the bottom of the box body 31. The card-type sample carrier 5 is fixed within the box body 31 via a slot on the inner wall of the box body 31. Specifically, the slots are a first groove 311 and a second groove 312 symmetrically arranged on the inner wall of the box body. The slots can hold one or more card-type sample carriers 5 along their height. Similarly, multiple sets of slots can also be provided within the box body 31.

[0041] Furthermore, the cover body is composed of an exhaust top cover disposed above the card-type sample carrier 5, and a first top cover 321 and a second top cover 322 symmetrically disposed on either side of the card-type sample carrier 5. The first top cover 321 and the second top cover 322 are each provided with a fluid application hole 34, which can interface with the first jet tube 43 and the second jet tube 44 in the tubular jet generator 4. The exhaust top cover includes a first exhaust cover 323 and a second exhaust cover 324 symmetrically disposed and respectively connected to the box body 31 via torsion spring hinges 33. The exhaust top cover acts as an exhaust device. The first exhaust cover 323 and the second exhaust cover 324 can be flipped open by the liquid nitrogen vapor during the jet impact to discharge the liquid nitrogen vapor, and can be closed after the jet impact ends and during transport to maintain the internal temperature of the low-temperature transport box 3.

[0042] Further, such as Figure 5 As shown, the tubular jet generator 4 includes a working fluid inlet connector 41, a diverter row 42 connected to the working fluid inlet connector 41, a first jet tube 43 and a second jet tube 44 connected to the diverter row 42, the diverter row 42 is fixed to the top surface of the experimental chamber 1 and the top surface is provided with a hole for the working fluid inlet connector 41 to pass through, the working fluid inlet connector 41 is connected to the liquid nitrogen tank 10 outside the experimental chamber 1 through a pipeline, the liquid nitrogen tank 10 is provided with an air pump, the first jet tube 43 and the second jet tube 44 are provided with jet holes 45 arranged in an array, the first jet tube 43 and the second jet tube 44 are respectively inserted into the working fluid action holes 34 on the first top cover 321 and the second top cover 322, and the jet holes 45 of the first jet tube 43 and the second jet tube 44 are aligned with the two surfaces of the card-type sample carrier 5. That is, after the first jet tube 43 and the second jet tube 44 are inserted into the low-temperature transport box 3, the card-type sample carrier 5 is located in the middle position between the first jet tube 43 and the second jet tube 44, and the jet holes 45 on the first jet tube 43 and the second jet tube 44 are facing the outer surface of the card-type sample carrier 5.

[0043] Furthermore, the first and second jet tubes 43, 44 are rigid tubes. In this embodiment, they are made of stainless steel. The ends of the first and second jet tubes 43, 44 are sealed, and the diameter of the jet holes 45 is 0.2-2 mm. Liquid nitrogen enters the diverter 42 through the working fluid inlet connector 41 and evenly flows into the first and second jet tubes 43, 44. It then flows through the jet holes 45 to evenly impact the outer surface of the card-type sample carrier 5. The microfluidic column of liquid nitrogen ejected through the jet holes 45 should completely cover the surface of the card-type sample carrier 5.

[0044] Further, such as Figure 6 、 Figure 7As shown, the card-type sample carrier 5 has a hollow structure, including a symmetrically arranged first thermal conductive sheet 51, a second thermal conductive sheet 52, and an intermediate layer 53 arranged between the first thermal conductive sheet 51 and the second thermal conductive sheet 52. The intermediate layer 53 is etched with a space or flow channel frame using a flexible thermal conductive material. The four corners of the outer surfaces of the first thermal conductive sheet 51 and the second thermal conductive sheet 52 are respectively embedded with attraction magnets 54. The attraction magnets 54 fix the first thermal conductive sheet 51, the second thermal conductive sheet 52 and the intermediate layer 53 through strong magnetic action to form a hollow structure. The hollow structure is used to carry biological samples such as hydrogel-encapsulated cells, microtissues or tissue / organ slices. A card-type sample carrier 5 can carry 0.483 ml of biological sample.

[0045] Furthermore, the first heat conducting sheet 51 and the second heat conducting sheet 52 are made of a high thermal conductivity material, and the outer surfaces of the first heat conducting sheet 51 and the second heat conducting sheet 52 are etched with bionic microchannels 55 to enhance the heat exchange effect. In this embodiment, the first heat conducting sheet 51 and the second heat conducting sheet 52 are made of aluminum nitride ceramic heat conducting sheets, and the bionic microchannels 55 are fork-shaped bionic microchannels. In actual use, the bionic microchannels 55 can also adopt other shapes such as Fibonacci circle channels, such as Figure 8 shown.

[0046] Further, such as Figure 9 、 Figure 10 As shown, the automatic lifting device 6 includes a screw transmission mechanism 61 placed outside the experimental chamber 1 and a workbench 62 placed inside the experimental chamber 1. The workbench 62 is used to place the low-temperature transfer box 3. The bottom of the workbench 62 is provided with a transmission sheet metal 63 connected to the slider in the screw transmission mechanism 61. The screw transmission mechanism 61 is driven by a stepper motor 64.

[0047] Furthermore, the workbench 62 is equipped with a positioning frame 621 that mates with the cryogenic transport box 3. This positioning frame 621 is used to secure the cryogenic transport box 3 and align the working medium application holes 34 thereon with the positions of the first and second fluidic tubes 43, 44. The walls of the experimental chamber 1 are provided with a limiting groove 11 that mates with the transmission sheet metal 63. This limiting groove 11 is vertically defined, and a stepper motor 64, via a slider, drives the transmission sheet metal 63 up and down along the direction defined by the limiting groove 11, thereby achieving the raising and lowering of the workbench 62.

[0048] Furthermore, if Figure 12As shown, a sealing isolation device is also provided in the experimental chamber 1. The sealing isolation device is arranged at a position corresponding to the limiting groove 11 and is used to seal and isolate the inside and outside of the experimental chamber 1. The sealing isolation device includes an isolation frame 71 fixed to the inner wall of the experimental chamber 1 and an accordion sealing strip 72 arranged in the isolation frame 71. The accordion sealing strip 72 can move up and down to form a seal as the transmission sheet metal 63 moves up and down. That is, the isolation frame 71 and the accordion sealing strip 72 form a pair of sealing isolation devices, isolating the workbench 62 from the stepper motor 64 and the screw transmission mechanism 61. They are only connected through the transmission sheet metal 63. While not affecting the transmission of the mechanism's motion, it can prevent liquid nitrogen vapor from entering the position of the screw transmission mechanism 61 and the stepper motor 64, causing the components to freeze and become stuck or affecting the operation of the stepper motor 64. Preferably, in this embodiment, the transmission sheet metal 63, the limiting groove 11, and the sealing isolation device are each provided in two groups, and the screw in the screw transmission mechanism 61 is a double screw.

[0049] Further, such as Figure 2 As shown, the experimental chamber 1 is further provided with a first pressing block 12 and a second pressing block 13. The positions of the first pressing block 12 and the second pressing block 13 coincide with the positions of the first top cover 321 and the second top cover 322, respectively. The first pressing block 12 and the second pressing block 13 are connected to the top surface of the experimental chamber 1 via spring pins 14. The first pressing block 12 and the second pressing block 13 are used to press down the low-temperature transport box 3 to prevent the low-temperature transport box 3 from moving during the jet impact.

[0050] The working principle of the present invention is as follows:

[0051] (1) Constant low temperature transport. The present invention uses a low temperature transport box to load the card-type sample carrier, and drives liquid nitrogen in the low temperature transport box to perform high-speed jet impact cooling on the card-type sample carrier. The unvaporized liquid nitrogen remains in the low temperature transport box to maintain the low temperature environment. During transportation, the low temperature transport box is directly transported as a whole to the storage station, which can avoid the card-type sample carrier being exposed to the air after the jet impact is completed and during the transportation process, causing temperature rise, recrystallization, and devitrification.

[0052] (2) High-throughput cooling structure. The present invention uses a tubular jet generator and a method for jet impact cooling in a low-temperature transport box, so that more card-type sample carriers can be loaded during each cooling. The throughput of biological materials during each jet impact cooling can be further increased by changing the number of tubular jet generators, the size of the card-type sample carriers, and the size of the low-temperature transport box.

[0053] The present invention can realize control and operation based on a working fluid drive and control system. The specific structure of the working fluid drive and control system can be referred to the prior art and will not be described in detail here.

[0054] The working process of the present invention is as follows:

[0055] First, the prepared cell fluid and liquid hydrogel are mixed and then evenly coated into the hollow structure of the card-type sample carrier 5. Excess cell fluid is gently scraped off and a solid hydrogel is allowed to form. The card-type sample carrier 5 is then fixed with the magnet 54 to form a package.

[0056] Second, open the exhaust cover on the low-temperature transport box 3, insert 1 to 3 card-type sample carriers 5 loaded with cell fluid into the card slot of the low-temperature transport box as needed, install the first cover 321 and the second cover 322 on both sides of the low-temperature transport box 3 and reset the exhaust cover.

[0057] Third, open the door of the experimental chamber 1 and insert the low-temperature transport box 3 containing the card-type sample carrier 5 into the positioning frame 621 on the workbench 62. After the installation is stable, close the door of the experimental chamber 1.

[0058] Fourth, start the working fluid drive and control system, turn on the air pump connected to the liquid nitrogen tank, increase the air pressure to 0.7MPa and then stop it for standby; open the liquid nitrogen tank cover, fill it with about 1L of liquid nitrogen and close the liquid nitrogen cover for standby.

[0059] Fifth, turn on the automatic lifting device 6, and first the stepping motor 64 drives the screw transmission mechanism 61 to lift the workbench. Since the positioning frame 621 limits the position of the low-temperature transport box 3, the jet tube on the tubular jet generator 4 can be aligned with the working medium action hole 34 on the low-temperature transport box 3 during the lifting process. Insert, when the first top cover 321 and the second top cover 322 of the low-temperature transport box 3 are in contact with the first pressure block 12 and the second pressure block 13 in the experimental chamber and push the first pressure block 12 and the second pressure block 13 upward and move 10 to 15 mm, the automatic lifting device 6 stops working. At this time, the top cover of the low-temperature transport box 3 is subjected to a certain extrusion force, which can prevent it from being opened when the liquid nitrogen vaporizes, and at the same time make the bottom of the jet tube on the tubular jet generator 4 almost flush with the inner bottom surface of the low-temperature transport box 3. At this time, the array jet hole position on the jet tube is facing the card-type sample carrier 5. Figure 13 shown.

[0060] Sixth, the working fluid drive and control system controls the air pump valve to open, and the high-pressure gas enters the liquid nitrogen tank to squeeze out the liquid nitrogen, and enters the working fluid inlet connector 41 of the tubular jet generator 4 through the pipeline. Then the liquid nitrogen flows to each jet tube through the diversion row 42, and is ejected at high speed through the array of jet holes on the jet tube, evenly covering and impacting the surface of the card-type sample carrier 5 at high speed, so that the thin film of cell fluid can be 10 4℃ / min high-speed cooling to below -150℃. The liquid nitrogen that hits the surface of the card-type sample carrier 5 at high speed will partially vaporize to form liquid nitrogen vapor. The large amount of liquid nitrogen that enters will form more liquid nitrogen vapor, causing the pressure inside the low-temperature transport box 3 to increase. At this time, the exhaust cover on the top of the low-temperature transport box 3 will be pushed open by this pressure, discharging the excess liquid nitrogen vapor. These gases will be discharged through the exhaust port 2 on the experimental chamber 1, as shown in the figure. Figure 14 、 Figure 15 shown.

[0061] Seventh, the working fluid drive and control system controls the air pump valve to open for 1.5 to 2 seconds and then close. At this time, the liquid nitrogen in the liquid nitrogen tank has been completely squeezed out, and the liquid nitrogen in the cryogenic transfer box 3 tends to be stable. The exhaust top cover is reclosed under the action of the torsion spring hinge. The unvaporized liquid nitrogen remains in the cryogenic transfer box and evaporates in small amounts, making the ambient temperature in the cryogenic transfer box lower than -150°C.

[0062] Eighth, after the jet is completed, the automatic lifting device 6 is started and the workbench 62 is gradually lowered. It stops after the jet tube is out of the working fluid action hole 34. At this time, the low-temperature transport box 3 is taken out from the workbench 62 and transported to the frozen cell bank, and then the card-type sample carrier 5 is quickly taken out and placed in the cryopreservation bank for freezing; since a certain amount of liquid nitrogen remains in the low-temperature transport box 3, the liquid nitrogen evaporates in a small amount to form liquid nitrogen vapor, and the excess and gradually heated gas is gradually discharged through the working fluid action hole 34 at the top, which can continuously keep the ambient temperature in the low-temperature transport box 3 below -150°C, avoiding recrystallization or devitrification due to the increase in ambient temperature during transportation.

[0063] Example 1:

[0064] In this embodiment, the box body 31 of the low-temperature transport box 3 is made of EPS foam and is a rectangular box body with an overall outer dimension of 185×100×115 mm and an inner dimension of 160×75×85 mm. A set of card slots is provided in the box body 31, and the height of the card slot is 60 mm. A card-type sample carrier 5 is placed in the card slot. The overall dimension of the card-type sample carrier 5 is 50×25×0.9 mm. A single card-type sample carrier 5 can load 0.483 ml of hydrogel-encapsulated biological material. The apertures of the working medium action holes 34 on the first top cover 321 and the second top cover 322 are both 12.5 mm, and the distance between the two holes is 80 mm. The dimensions of the workbench 62 are 185×2 50mm, the dimensions of the positioning frame 621 thereon are 185×100×115mm; the screw drive mechanism 61 can be raised and lowered from 0 to 220mm; the first jet tube 43 and the second jet tube 44 have a diameter of 12mm, and have jet holes 45 with a diameter of 1mm. The spacing between the first jet tube 43 and the second jet tube 44 is 80mm, the height of the array jet holes is 60mm, and the maximum angle between the left and right ends is 80°. The microfluidic column of liquid nitrogen spray can cover an area of ​​approximately 67×65mm at the card-type sample carrier 5, which can completely cover the surface of the card-type sample carrier 5. The biological sample port injected into the card-type sample carrier 5 contains diluted bone marrow mesenchymal stem cell liquid.

[0065] The structure of the above embodiment is used to perform ultra-high-speed jet impact cooling, and T-type thermocouples are used to record temperature data at three different points in the card-type sample carrier 5. After sorting, the test data are as follows: Figure 18 As shown, it can be seen that the cooling rate of the biological sample can reach 10 4 ℃ / min or above, and after the jet impact is completed and the low-temperature transport box 3 is taken out, the temperature of the biological sample is always maintained below -150℃.

[0066] Example 2:

[0067] like Figure 16 、 Figure 17 As shown, in order to increase the biomaterial flux of each jet impact cooling, two groups of card slots can be set inside the low-temperature transport box of the present invention, and the card slot height is 70mm. Two card-type sample carriers can be inserted into each group of card slots; correspondingly, the cover of the low-temperature transport box is provided with three working fluid action holes, and a group of exhaust top covers are provided between two adjacent working fluid action holes, and the first top cover and the second top cover are respectively provided on both sides; correspondingly, the tubular jet generator is provided with three jet tubes, and the jet tubes on both sides have 0.8mm array jet holes on the side facing the card-type sample carrier, and the middle jet tube has array jet holes symmetrically opened on both sides, so that the four card-type sample carriers in the two groups of card slots can be impact cooled.

[0068] The embodiments described above are merely descriptions of preferred implementations of the present invention and are not intended to limit the scope of the present invention. Without departing from the spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should fall within the scope of protection determined by the claims of the present invention.

Claims

1. A high-throughput, high-speed cooling and transport system, characterized by: The invention comprises a sealed experimental chamber (1), wherein the experimental chamber (1) is provided with an exhaust port (2) communicating with the outside, wherein a low-temperature transport box (3) and a tubular jet generator (4) are provided in the experimental chamber (1), and wherein the low-temperature transport box (3) is driven by an automatic lifting device (6) to move up and down in the experimental chamber (1) to achieve connection or disconnection between the low-temperature transport box (3) and the tubular jet generator (4); The low-temperature transport box (3) comprises a square box body (31) and a cover body matched with the box body (31); a card-type sample carrier (5) is provided in the box body (31), the card-type sample carrier (5) is arranged along the width direction of the box body and has a gap with the bottom of the box body (31); the card-type sample carrier (5) is fixed in the box body (31) through a slot on the inner wall of the box body (31); the cover body is composed of an exhaust top cover arranged above the card-type sample carrier (5) and a first top cover (321) and a second top cover (322) symmetrically arranged on both sides of the card-type sample carrier (5); the exhaust top cover comprises a first exhaust cover (323) and a second exhaust cover (324) symmetrically arranged and connected to the box body (31) through a torsion spring hinge (33); the first top cover (321) and the second top cover (322) are respectively provided with a working medium action hole (34); The tubular jet generator (4) includes a working fluid inlet joint (41), a diverter row (42) connected to the working fluid inlet joint (41), a first jet tube (43) and a second jet tube (44) connected to the diverter row (42), the diverter row (42) is fixed on the top surface of the experimental chamber (1) and the top surface is provided with a hole for the working fluid inlet joint (41) to pass through, the working fluid inlet joint (41) is connected to the liquid nitrogen tank outside the experimental chamber (1) through a pipeline, the first jet tube (43) and the second jet tube (44) are provided with jet holes (45) arranged in an array, the first jet tube (43) and the second jet tube (44) are respectively inserted into the working fluid action holes (34) on the first top cover (321) and the second top cover (322), and the jet holes (45) of the first jet tube (43) and the second jet tube (44) are aligned with the two surfaces of the card-type sample carrier (5); The automatic lifting device (6) includes a screw transmission mechanism (61) placed outside the experimental chamber (1) and a workbench (62) placed inside the experimental chamber (1), wherein the workbench (62) is used to place the low-temperature transfer box (3), and the bottom of the workbench (62) is provided with a transmission sheet metal (63) connected to a slider in the screw transmission mechanism (61), and the screw transmission mechanism (61) is driven by a stepping motor (64); The card-type sample carrier (5) is a hollow structure, comprising a symmetrically arranged first thermal conductive sheet (51), a second thermal conductive sheet (52), and an intermediate layer (53) arranged between the first thermal conductive sheet (51) and the second thermal conductive sheet (52). The intermediate layer (53) is etched with a space or a flow channel frame using a flexible thermal conductive material. The four corners of the outer surfaces of the first thermal conductive sheet (51) and the second thermal conductive sheet (52) are respectively embedded with attractive magnets (54). The attractive magnets (54) fix the first thermal conductive sheet (51), the second thermal conductive sheet (52) and the intermediate layer (53) through a strong magnetic effect to form a hollow structure. The hollow structure is used to carry the biological sample encapsulated in the hydrogel.

2. The high-throughput, high-speed cooling and transport system according to claim 1, characterized in that: The first heat conducting plate (51) and the second heat conducting plate (52) are made of a high heat conducting material, and the outer surfaces of the first heat conducting plate (51) and the second heat conducting plate (52) are etched with bionic microchannels (55) that enhance the heat exchange effect.

3. The high-throughput, high-speed cooling and transport system according to claim 1, characterized in that: The card slots are a first slot (311) and a second slot (312) symmetrically arranged on the inner wall of the box body, and the card slots can fix one or more card-type sample carriers (5) along the height direction.

4. The high-throughput, high-speed cooling and transport system according to claim 1, characterized in that: The first jet tube (43) and the second jet tube (44) are rigid tubes, and the ends of the first jet tube (43) and the second jet tube (44) are sealed. The aperture of the jet hole (45) is 0.2 to 2 mm. Liquid nitrogen enters the diversion row (42) through the working medium inlet joint (41) and evenly flows into the first jet tube (43) and the second jet tube (44), and then evenly impacts the outer surface of the card-type sample carrier (5) through the jet hole (45).

5. The high-throughput, high-speed cooling and transport system according to claim 1, characterized in that: The workbench (62) is provided with a positioning frame (621) that matches the low-temperature transfer box (3), and the cavity wall of the experimental cavity (1) is provided with a limiting groove (11) that matches the transmission sheet metal (63). The limiting groove (11) is opened in the vertical direction. The stepping motor (64) drives the transmission sheet metal (63) to move up and down along the direction defined by the limiting groove (11) through the slider, thereby realizing the lifting and lowering of the workbench (62).

6. The high-throughput, high-speed cooling and transport system according to claim 5, characterized in that: The experimental chamber (1) is further provided with a sealing isolation device, which is arranged at a corresponding position of the limiting groove (11) and is used to seal and isolate the inside and outside of the experimental chamber (1). The sealing isolation device includes an isolation frame (71) fixed on the inner wall of the experimental chamber (1) and an accordion sealing strip (72) arranged in the isolation frame (71). The accordion sealing strip (72) can be extended and retracted up and down to form a seal as the transmission sheet metal (63) moves up and down.

7. The high-throughput, high-speed cooling and transport system according to claim 6, characterized in that: The transmission sheet metal (63), the limiting groove (11), and the sealing isolation device are respectively provided in two groups, and the screw in the screw transmission mechanism (61) is a double screw.

8. The high-throughput, high-speed cooling and transport system according to claim 1, characterized in that: The experimental chamber (1) is further provided with a first pressing block (12) and a second pressing block (13), the positions of the first pressing block (12) and the second pressing block (13) respectively correspond to the positions of the first top cover (321) and the second top cover (322), and the first pressing block (12) and the second pressing block (13) are respectively connected to the top surface of the experimental chamber (1) via spring pins (14).

9. The high-throughput, high-speed cooling and transport system according to claim 1, characterized in that: The low-temperature transport box (3) is made of heat-insulating material.

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