A transportation device and method for improving the survival rate of Lutjanus erythropterus fry

Abstract

The present invention belongs to the technical field of fish transportation, and discloses a transportation device and a transportation method for improving the survival rate of Lutjanus erythopterus fry. The transportation device includes a transportation water tank, as well as a shock-absorbing component, a light intensity mechanism, an oxygenation mechanism and a solar energy mechanism arranged in the transportation water tank; the shock-absorbing component is made of EVA foam and has a structure of two stacked triangular pyramids. The shock-absorbing component is a bidirectional conical structure, which can slow down the water flow impact and vibration from different directions of up and down, left and right, and front and back. The shock-absorbing component is designed to be slidable up and down, facilitating use under different transportation water depth conditions. At the same time, the shock-absorbing component can exist as an artificial fish reef structure in water, enriching the living environment of Lutjanus erythopterus during transportation while reducing shock, thereby improving the transportation survival rate.

Description

Technical Field

[0001] The present invention belongs to the technical field of fish transportation, and more specifically relates to a transportation device and a transportation method for improving the survival rate of Lutjanus erythropterus fry. Background Art

[0002] Lutjanus erythropterus belongs to Perciformes, Lutjanidae, Lutjanus, and is a warm-water demersal fish distributed in the western Pacific Ocean, the Indian Ocean and the South China Sea. It is an important marine economic fish with delicious meat and rich in protein and other nutritional values, and has become an important aquaculture object in the southern coastal areas of China and Southeast Asia. During the aquaculture process of Lutjanus erythropterus fry, long-distance transportation is often encountered. When transporting, the hatchery or fry farm is generally located far from fish ponds, cages and farms. It takes a long time to transport by car, from several hours to several days to reach the destination. The key to successful fry transportation is to do a good job in the preparatory work before transportation, which is the guarantee for improving the survival rate of fish during transportation.

[0003] The fry transportation methods include two types: closed transportation and open transportation. Closed transportation mainly includes plastic bag oxygen filling and sealing transportation and plastic barrel oxygen filling transportation. Open transportation mainly uses canvas box (barrel) transportation. During transportation, air (oxygen) needs to be continuously filled or the water surface needs to be struck with a water striking board to increase the dissolved oxygen in the water. Both transportation methods ensure the supply of oxygen. However, during transportation, in addition to ensuring the oxygen supply, affected by the water body surging caused by vehicle vibration, the fry are affected by external forces such as mutual collision and extrusion under the oscillation effect. Excessive external force will cause fish body damage and physiological function disorders, affect the fish meat quality, and even lead to death in severe cases. And Lutjanus erythropterus is a reef-dwelling fish, and the transportation process has not considered improving its transportation efficiency from the perspective of enriching the habitat environment. Therefore, it is urgent to take effective measures to reduce large-scale damage or death caused by mutual impact between fry during transportation. At the same time, when transporting Lutjanus erythropterus fry, the transportation carriage is generally in a long-term dark state, and the light difference between before and after transportation and during transportation is relatively large. This long-term darkness and inappropriate photoperiod will have an adverse impact on the activities, growth and development of the fry.

[0004] Therefore, how to provide a transportation device and a transportation method for improving the survival rate of Lutjanus erythropterus fry, reducing water body vibration and reducing the degree of fry damage is an urgent problem to be solved by those skilled in the art. Summary of the Invention

[0005] In order to overcome the shortcomings and deficiencies in the prior art, the present invention provides a transportation device and a transportation method for improving the survival rate of Lutjanus erythropterus fry.

[0006] In order to achieve the above purpose, the present invention adopts the following technical solutions:

[0007] A transportation device for improving the survival rate of Lutjanus erythopterus fry, comprising a transportation water tank, and a shock-absorbing component, a light intensity mechanism, an oxygenation mechanism and a solar energy mechanism arranged in the transportation water tank;

[0008] The transportation water tank is a square box, with a stainless steel frame support arranged inside the box body, and the water depth during transportation does not exceed 3 / 4 of the height of the water tank;

[0009] The shock-absorbing component is made of EVA foam and has a structure of two stacked triangular pyramids with their bottom surfaces coinciding; three connecting rings are fixed at the three intersection angles of the shock-absorbing component, and three slidable connecting rings are arranged on the stainless steel frame of the transportation water tank. The slidable connecting rings are connected to the connecting rings of the shock-absorbing component through connecting lines, and the fixed height of the shock-absorbing component can be adjusted through the slidable connecting rings.

[0010] The beneficial effects of the above technical solution are as follows: Each direction of the shock-absorbing component presents in the form of an angle, and the structure of the pyramid with each angle gradually increasing and then decreasing along the boundary is streamline-shaped, which reduces the flow velocity and impact force of the water flow in the water tank and protects the fish in the water tank. At the same time, since Lutjanus erythopterus is a reef-dwelling fish and prefers a habitat environment with reef bodies, the shock-absorbing component is placed inside the water tank and can exist as an artificial reef structure in the water. It can not only reduce shock but also enrich the living environment of Lutjanus erythopterus during transportation, thereby improving the transportation survival rate.

[0011] Preferably, the connecting line has a certain stretching strength to ensure that the shock-absorbing component can swing up, down, left and right under the action of water flow.

[0012] The beneficial effects of the above technical solution are as follows: The goal of slowing down the back-and-forth oscillation of the water body in the water tank is achieved.

[0013] Preferably, the total length of the connecting line and the connecting ring is controlled to avoid being too long and causing the shock-absorbing component to hit the side wall of the transportation water tank during the swinging process.

[0014] Preferably, the light intensity mechanism includes a white light strip and an adjustable switch; the white light strip is fixed around the top of the stainless steel frame, and the adjustable switch is fixed on the top edge of the transportation water tank.

[0015] Preferably, the oxygenation mechanism includes an air pump and a pipeline extending to the bottom of the transportation water tank; the air pump is fixed on the outer side wall of the transportation water tank and is connected to the pipeline.

[0016] Preferably, both the light intensity mechanism and the oxygenation mechanism are connected to the solar energy mechanism through wires, and the solar energy mechanism is fixed on the outside of the transportation water tank.

[0017] Preferably, circular fixing rings are arranged at the 8 top corners on the outside of the transportation water tank, which can fix the transportation water tank in the transportation vehicle.

[0018] A transportation method for improving the survival rate of Lutjanus erythopterus fry, using the above-mentioned transportation device, includes the following steps:

[0019] (1) Add filtered seawater into the transportation water tank, continuously aerate it with an air stone, and detect the water environment parameter indicators of the transportation water tank to ensure that the water environment of the transportation water tank is consistent with the aquaculture environment;

[0020] (2) Adjust the shock-absorbing component below the water surface, then fix it, turn on the light intensity mechanism, and set the light intensity;

[0021] (3) Place the Lutjanus erythopterus fry in the transportation water tank for transportation.

[0022] Preferably, the water environment parameter indicators in step (1) include pH, salinity, temperature, dissolved oxygen, and ammonium nitrogen concentration.

[0023] Preferably, the light intensity in step (2) is 100 lx.

[0024] Preferably, the transportation density in step (3) is 1 kg / m 3 .

[0025] Through the above technical solutions, compared with the prior art, the present invention provides a transportation device and a transportation method for improving the survival rate of Lutjanus erythopterus fry, having the following beneficial effects:

[0026] 1. According to the phenomenon of fish colliding with each other caused by water body oscillation during transportation, the present invention designs a transportation device with a shock-absorbing component that is simple to operate and has a low cost. The shock-absorbing material is easily obtained and will not have an adverse impact on fish, and it is not easily deformed. The shock-absorbing structure is designed as a double-cone structure to slow down the water flow impact and oscillation from different directions, such as up and down, left and right, and front and back. The shock-absorbing component is designed to be slidable up and down, facilitating its use under different transportation water depth conditions. The transportation device can be flexibly moved and installed in each transportation carriage. The setting of the solar panel can provide lasting power for the light intensity mechanism and the oxygenation mechanism to ensure their normal operation.

[0027] 2. The present invention proposes to use the shock-absorbing component as a way to enrich the transportation habitat environment of Lutjanus erythopterus, and at the same time, the view of supplementing the light intensity to the fry transportation process, evaluates the optimal light intensity under the transportation conditions of Lutjanus erythopterus, and proposes a method for reducing the transportation stress response of Lutjanus erythopterus and improving the transportation survival rate according to the optimal light intensity. The adjustable light intensity mechanism is added to the transportation device to provide an optimal light intensity screening device and a long-distance transportation facility for the transportation of different species and specifications of fish. Description of the Drawings

[0028] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the accompanying drawings required in the description of the embodiments or the prior art. Obviously, the accompanying drawings in the following description are only the embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other accompanying drawings can also be obtained based on the provided drawings.

[0029] Figure 1 Stereogram of the transportation device of the present invention;

[0030] Figure 2 Top view of the transportation device of the present invention;

[0031] Figure 3 Left view of the transportation device of the present invention;

[0032] Figure 4 Average values of CAT and POD under different light intensities;

[0033] Figure 5 Average values of T-AOC and ACP under different light intensities;

[0034] Figure 6 Average values of LDH and AMS under different light intensities;

[0035] Figure 7 Average values of LPS and ATP under different light intensities;

[0036] Wherein: 1 - transportation water tank, 2 - stainless steel frame, 3 - white light strip, 4 - adjustable switch, 5 - shock-absorbing component, 6 - connecting ring, 7 - connecting wire, 8 - slidable connecting ring, 9 - pipeline, 10 - air pump, 11 - circular fixing ring, 12 - solar mechanism. Detailed implementation manners

[0037] The following will clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of them. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0038] Embodiment 1

[0039] A transportation device for improving the survival rate of red snapper fry, including a transportation water tank, and a shock-absorbing component, a light intensity mechanism, an oxygen-increasing mechanism and a solar mechanism arranged in the transportation water tank;

[0040] The transportation water tank is a square box, and a stainless steel frame is arranged inside the box for support. During transportation, the water depth does not exceed 3 / 4 of the height of the water tank;

[0041] The shock-absorbing component is made of EVA foam and has a structure of two stacked triangular pyramids with their bases coinciding; three connecting rings are fixed at the three intersection angles of the shock-absorbing component, and three slidable connecting rings are provided on the stainless steel frame of the transportation water tank. The slidable connecting rings are connected to the connecting rings of the shock-absorbing component through connecting lines, and the fixed height of the shock-absorbing component can be adjusted through the slidable connecting rings.

[0042] The connecting line has a certain stretching strength to ensure that the shock-absorbing component can sway up, down, left, and right under the action of water flow.

[0043] Control the total length of the connecting line and the connecting ring to avoid being too long and causing the shock-absorbing component to hit the side wall of the transportation water tank during swaying.

[0044] The light intensity mechanism includes a white light strip and an adjustable switch; the white light strip is fixed around the top of the stainless steel frame, and the adjustable switch is fixed on the top edge of the transportation water tank.

[0045] The oxygenation mechanism includes an air pump and a pipeline extending to the bottom of the transportation water tank; the air pump is fixed on the outer side wall of the transportation water tank and is connected to the pipeline.

[0046] Both the light intensity mechanism and the oxygenation mechanism are connected to the solar energy mechanism through circuits, and the solar energy mechanism is fixed outside the transportation water tank.

[0047] Circular fixing rings are provided at the 8 top corners on the outer side of the transportation water tank, which can fix the transportation water tank in the transportation vehicle.

[0048] Example 2

[0049] Adopt the transportation device of Example 1, and preferably a transportation method for improving the survival rate of Lutjanus erythopterus fry:

[0050] Add filtered seawater into the transportation water tank, keep the water level at 0.75 m, continuously inflate with an air stone, and set the simulated transportation time to 8 hours. The Lutjanus erythopterus breeding environment is set indoors, and the breeding density is set to 1 kg / m 3 , and the water quality parameters during the breeding process are salinity (33.00 ± 0.80), temperature (27.00 ± 0.50) °C, ammonium nitrogen concentration less than 0.01 mg / L, nitrite mass concentration less than 0.04 mg / L, and dissolved oxygen concentration greater than 6.50 mg / L. Feed the fish twice a day during the breeding period, at 9:00 AM and 17:00 PM respectively, change 50% of the seawater every day, and stop feeding one day before the experiment.

[0051] The shock-absorbing component was adjusted below the water surface. When the water covered the shock-absorbing component completely, it was fixed. The light intensity mechanism was turned on and the light intensity was set to 0, 100, 500, 1000, and 5000 lx, respectively. The 0 lx group was used as the control group. Disease-free, healthy, and uniform redfin snapper seedlings were selected and placed in the transport water tank. Six redfin snappers that had not undergone the transport test were randomly selected for the experiment. The body length was (12.00 ± 0.60) cm / fish, the fish weight was (10 ± 0.50) g / fish, and the transport density was 1 kg / m 3 Three parallel groups were set up for each of the five light intensity groups, named groups 1-3. The experiment started at 8:00 AM and the simulated transport ended at 16:00 PM. At the beginning of the experiment, the salinity, dissolved oxygen, ammonia nitrogen, and pH values ​​of the water in the box were measured to be 30.2‰, 7.02 mg / L, 3.19 mg / L, and 8.32, respectively.

[0052] After the transport test of five light intensities, the pH, salinity, temperature, dissolved oxygen, ammonium nitrogen concentration and nitrite concentration of the transport water were measured and recorded immediately. At the same time, six fish were randomly selected as whole fish samples. After anesthesia with an appropriate amount of eugenol, they were dissected on crushed ice and liver, gill and intestinal tissue samples were removed. They were weighed with a micrometer balance, and 9 times 0.86% saline was added at a ratio of 1:9 (g / mL). After grinding with a grinder, they were placed in a refrigerated centrifuge and centrifuged at 2500r / min for 1 minute. The supernatant was collected and frozen in a -80℃ refrigerator. The liver, gill and intestinal tissue samples of the extracted redfin snapper were then tested and analyzed for acid phosphatase (ACP), catalase (CAT), peroxidase (POD), total antioxidant enzyme (T-AOC), lactate dehydrogenase (LDH), adenosine triphosphatase (ATP) in the gills, and lipase (LPS) and α-amylase (AMS) in the intestine. The data results are shown in Table 1. Figures 4-7 shown.

[0053] Table 1 Tissue enzyme activity data

[0054]

[0055]

[0056] The results showed that when the light intensity was 100 lx, the dissolved oxygen and liquid ammonia concentration were significantly higher than those in other light intensity groups. The activities of catalase, peroxidase and total antioxidant enzyme were the lowest under the condition of 100 lx, and those in the dark and other light intensity groups were higher than this condition, indicating that when in the dark and under very high light intensity, the stress of fry was severe. The activities of acid phosphatase in the 1000 lx and 5000 lx light intensity groups were significantly higher than those under the conditions of 100 lx and 500 lx, indicating that the stress degree of the organism was higher under high-intensity light conditions, while the activity of acid phosphatase was the highest under dark conditions, indicating that the stress situation of Lutjanus erythropterus was the most severe under this condition. The activities of lactate dehydrogenase under the light intensities of 500 lx, 1000 lx, 5000 lx and in the dark were all significantly higher than those in the 100 lx group, indicating that the liver of fry was indeed damaged during transportation, and it would only remain at a lower level under the light intensity of 100 lx. The activity of α-amylase was the highest under dark conditions, indicating that the energy consumption was the fastest under this condition. The activities in the 100 lx and 500 lx light intensity groups were significantly lower than those in other groups, indicating that not much energy was consumed under this condition and it was more suitable for transportation. However, there were no significant differences in the lipase activities among the five groups, indicating that the organism did not consume too much fat and did not cause an increase in lipase activity. The activities of adenosine triphosphatase under dark conditions were all significantly lower than those in other groups, indicating that under this condition, fish were in a state of high-intensity stress, more energy was consumed in the body, and functions such as fish respiration, ammonia nitrogen excretion and osmotic pressure regulation were intensified. While the activities of adenosine triphosphatase were significantly higher than those in other groups when the light intensity was 100 lx, indicating that under this condition, the stress on fry was smaller and it was more suitable for transportation.

[0057] The research results indicate that Lutjanus erythropterus fry are more suitable for transportation under the condition of a light intensity of 100 lx.

[0058] In this specification, each embodiment is described in a progressive manner. The key point of each embodiment is to illustrate the differences from other embodiments. For the same or similar parts among the embodiments, reference can be made to each other. For the solutions disclosed in the embodiments, since they correspond to the methods disclosed in the embodiments, the description is relatively simple, and reference can be made to the method part for the relevant parts.

[0059] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be obvious to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to these embodiments shown herein, but will be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A transportation device for improving the survival rate of Lutjanus erythropterus fry, characterized in that, It includes a transportation water tank, as well as a shock-absorbing component, a light intensity mechanism, an aeration mechanism, and a solar energy mechanism arranged inside the transportation water tank; The transportation water tank is a square box, with a stainless-steel frame supported inside the box. During transportation, the water depth does not exceed 3 / 4 of the height of the water tank; The shock-absorbing component is made of EVA foam and has a structure of two stacked triangular pyramids with their bottom surfaces coinciding. Three connecting rings are fixed at the three intersection angles of the shock-absorbing component. Three slidable connecting rings are provided on the stainless-steel frame of the transportation water tank. The slidable connecting rings are connected to the connecting rings of the shock-absorbing component through connecting lines, and the fixed height of the shock-absorbing component can be adjusted through the slidable connecting rings; The connecting line has a certain stretching strength to ensure that the shock-absorbing component can swing up, down, left, and right under the action of water flow.

2. The transportation device for improving the survival rate of Lutjanus erythopterus fry according to claim 1, characterized in that, The light intensity mechanism includes a white light strip and an adjustable switch; the white light strip is fixed around the top of the stainless-steel frame, and the adjustable switch is fixed on the top edge of the transportation water tank.

3. The transportation device for improving the survival rate of Lutjanus erythropterus fry according to claim 1, characterized in that, The aeration mechanism includes an air pump and a pipeline extending to the bottom of the transportation water tank; the air pump is fixed on the outer side wall of the transportation water tank and is connected to the pipeline.

4. The transportation device for improving the survival rate of Lutjanus erythopterus fry according to claim 1, characterized in that, Both the light intensity mechanism and the aeration mechanism are connected to the solar energy mechanism through circuits, and the solar energy mechanism is fixed on the outside of the transportation water tank.

5. The transportation device for improving the survival rate of red snapper fry according to claim 1, characterized in that, Circular fixing rings are provided at the 8 top corners on the outside of the transportation water tank, which can fix the transportation water tank inside the transportation vehicle.

6. A transportation method for improving the survival rate of juvenile Lutjanus erythopterus, characterized in that, Using the transportation device according to any one of claims 1-5, it includes the following steps: (1) Add filtered seawater into the transportation water tank, continuously aerate with an air stone, and detect the water environment parameter indicators of the transportation water tank to ensure that the water environment of the transportation water tank is consistent with the aquaculture environment; (2) Adjust the shock-absorbing component below the water surface, then fix it, turn on the light intensity mechanism, and set the light intensity; (3) Place the larvae of Lutjanus erythopterus in the transportation water tank for transportation.

7. The transportation method for improving the survival rate of red snapper fry according to claim 6, characterized in that, The water environment parameter indicators described in step (1) include pH, salinity, temperature, dissolved oxygen, and ammonium nitrogen concentration.

8. A transportation method for improving the survival rate of red snapper fry according to claim 6, characterized in that The light intensity described in step (2) is 100 lx.

9. The transportation method for improving the survival rate of red snapper fry according to claim 6, characterized in that, In step (3), the transportation density is 1 kg / m 3 .

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