Special medicine for treating adolescent acne and its preparation process

Abstract

This application relates to the field of traditional Chinese medicine compositions, specifically disclosing a special medicine for treating adolescent acne and its preparation process. The preparation process comprises the following steps: Step 1: pulverize turmeric and extract with steam distillation to obtain a turmeric single extract and turmeric residue; pulverize coral ginger and extract with steam distillation to obtain a coral ginger single extract and coral ginger residue; pulverize Gynostemma pentaphyllum, Solidago asiatica, and Paris polyphylla into fine powders, mix them with the turmeric residue and coral ginger residue, and macerate with ethanol to collect the percolate; decoct Radix Angelicae Dahuricae and Schizonepeta tenuifolia with water, filter, and concentrate the filtrate to obtain a clear paste, add ethanol, let stand, and filter to obtain an alcohol precipitate; the alcohol precipitate and the percolate are mixed to obtain a mixed solution; Step 2: emulsify the turmeric single extract and coral ginger single extract, respectively, and mix them with the mixed solution to obtain the special medicine. This medicine has the advantages of improving the therapeutic effect on acne while further reducing pigmentation.

Description

Technical Field

[0001] The present application relates to the field of Chinese medicine compositions, and more specifically, to a special medicine for treating adolescent acne and its preparation process. Background Art

[0002] Acne is the most common chronic inflammatory disease of the hair follicles and sebaceous glands in dermatology. It occurs most commonly on the cheeks, forehead, and jawline, but can also affect the trunk, such as the chest, back, and scapula. Characterized by comedones, papules, pustules, nodules, cysts, and scars, it is often accompanied by seborrhea. It is common in adolescent boys and girls and is commonly referred to as "acne." Acne is a common inflammatory disease. The inflammatory process stimulates excessive melanin production, leading to abnormal melanin deposition. This inflammatory-induced skin pigmentation disorder causes hyperpigmentation in acne sufferers. Post-acne hyperpigmentation seriously impacts patients' quality of life.

[0003] In the treatment of acne, Western medicine often uses drugs such as antibiotics, isotretinoin, estrogen, corticosteroids, antibiotics, retinoic acid, benzoyl peroxide, estrogen, corticosteroids, and zinc salts. While these drugs can achieve some efficacy, they can also be irritating. Traditional Chinese medicine compositions have a long history of proven safety and can be tailored to the patient's specific needs and symptoms, making them more tailored to their specific needs. Different Chinese herbal medicines have synergistic effects and work together to exert therapeutic effects, so they are more effective than single drugs. Currently, the Chinese herbal components commonly used in the treatment of acne include: Gardenia, mint, rhubarb, honeysuckle, coptis, phellodendron, scutellaria, platycodon, licorice, turmeric, parsley, angelica sinensis, goldenseal, etc. The synergistic therapeutic effect is achieved by combining multiple Chinese medicines; when some of the above Chinese herbal components are combined to treat acne, the improvement of pigmentation caused by inflammation is not very good, so improving pigmentation while ensuring a good therapeutic effect on acne is an important direction worthy of research. Summary of the Invention

[0004] In order to improve the therapeutic effect of acne and further reduce pigmentation, the present application provides a special medicine for treating adolescent acne and its preparation process.

[0005] In a first aspect, the present application provides a preparation process for a special medicine for treating adolescent acne, which adopts the following technical solution:

[0006] A preparation process of a special medicine for treating adolescent acne, wherein the special medicine raw materials include: turmeric, Paris polyphylla, Radix Angelicae Dahuricae, Schizonepeta tenuifolia, Solidago asiatica, Gynostemma pentaphyllum, and Ginger coral.

[0007] The preparation process includes the following steps:

[0008] Step 1: Grind turmeric into coarse powder, and extract by steam distillation to obtain turmeric single extract and turmeric residue;

[0009] Grinding the coral ginger into coarse powder, and extracting by steam distillation to obtain the coral ginger single extract and coral ginger residue;

[0010] Gynostemma pentaphyllum, Solidago asiatica and Paris polyphylla were ground into fine powder and then mixed with the above-mentioned turmeric residue and coral ginger residue, soaked in ethanol and then percolated, and the percolated liquid was collected;

[0011] Decoction of Radix Angelicae Dahuricae and Herba Schizonepetae with water for three times, combining the decoctions and filtering to obtain a filtrate, concentrating the filtrate to obtain a clear paste, adding ethanol, allowing to stand, and filtering to obtain an alcohol precipitate; mixing the alcohol precipitate with the percolate to obtain a mixed solution;

[0012] Step 2: Polysorbate is added to the turmeric single extract and the coral ginger single extract respectively for emulsification, and then mixed with the mixed solution to obtain a special medicine for treating adolescent acne.

[0013] By adopting the above technical solution, the raw materials are prepared and compounded using the above process. Among them, turmeric: clears heat and dampness, dispels wind and relieves itching, and can treat acne caused by damp-heat stagnation, and can also be used to treat seborrheic dermatitis. Paris polyphylla: has multiple benefits, including antibacterial, anti-inflammatory, antiviral, analgesic, antipruritic, and eczema treatment. Paris polyphylla has significant therapeutic effects on acne. Because it is a topical medication, it has minimal side effects. Transdermal administration allows the drug to reach the lesion directly, making it safe, efficient, and easy to absorb. Rhizoma Coptidis: can treat skin diseases such as eczema, acne, and boils, and can effectively reduce inflammation and relieve pain. Herba Schizonepetae: has anti-inflammatory and bactericidal effects, killing bacteria on the skin surface while improving blood circulation and lowering blood lipids. Goldenrod: has the effects of clearing heat and detoxifying, dispelling wind and relieving itching, and can be used to treat skin inflammation and itching. Gynostemma pentaphyllum: has the effects of clearing heat and detoxifying, replenishing qi and nourishing blood, and resolving phlegm and relieving cough, and can be used to treat acne caused by qi and blood deficiency. Coral ginger: It has the effects of promoting blood circulation, removing blood stasis, reducing swelling and relieving pain, and can be used to treat acne caused by blood stasis. Curcumin, the active ingredient in turmeric, has antioxidant, anti-inflammatory, and antibacterial properties.

[0014] Curcumin can inhibit the growth of Propionibacterium acnes, thereby reducing inflammation and infection. It can also promote the regeneration and repair of skin cells, helping to alleviate the inflammation and scars caused by acne. Curcumin and ligustrine can inhibit the catalytic action of enzymes, thereby reducing the activity of tyrosinase, inhibiting the gene expression of tyrosinase, and further reducing the synthesis of tyrosinase, thereby inhibiting the production of melanin and reducing pigmentation. The above preparation method can effectively improve the extraction rate of curcumin, further improve the therapeutic effect on acne, and reduce pigmentation.

[0015] By adopting the above preparation process, volatile oils from turmeric and coral ginger are extracted by steam distillation, so that the volatile components of these medicinal materials can be retained and their medicinal effects can be exerted in the preparation; after the gynostemma pentaphyllum, solidago asiatica and parsley are crushed into fine powder, the powder is combined with the medicinal residue after oil extraction, and then soaked in 80% ethanol and slowly percolated, so that the effective components in the medicinal materials can be further extracted, thereby improving the efficacy of the preparation; the volatile oil is mixed with polysorbate 80 and then added to the medicinal solution, and mixed evenly, so as to prepare a stable emulsified system and make the preparation more stable; the obtained special medicine has the characteristics of complete extraction, stability and long-lasting medicinal effect, further improving the therapeutic effect on acne and reducing pigmentation.

[0016] Preferably, the weight parts of the special medicine raw materials are as follows: the weight parts of the special medicine raw materials are as follows: 37-63 parts of turmeric, 37-63 parts of Paris polyphylla, 37-63 parts of Radix Angelicae Pubescentis, 19-32 parts of Schizonepeta tenuifolia, 19-32 parts of Solidago asiatica, 19-32 parts of Gynostemma pentaphyllum, and 37-63 parts of Ginger coral.

[0017] By adopting the above technical solution and compounding the above raw materials in the above ratios, the therapeutic effect on adolescent acne can be further improved.

[0018] Preferably, the turmeric single extract in step 2 needs to undergo the following pretreatment before emulsification:

[0019] S1: dissolving glucomannan in water to obtain an aqueous solution; dissolving polyacrylic acid and stearic acid in ethanol, then adding sodium lauryl sulfate and stirring evenly to obtain an alcohol solution; mixing the aqueous solution and the alcohol solution and stirring to react to obtain a reaction solution; S2: mixing a turmeric single extract with the reaction solution and stirring evenly, heating the reaction and then cooling to obtain a modified turmeric single extract; adding polysorbate to the modified turmeric single extract and the coral ginger single extract respectively to emulsify them, and then mixing with the mixed solution to obtain a special medicine for treating adolescent acne.

[0020] While curcumin can inhibit the growth of Propionibacterium acnes, thereby reducing inflammation and infection, promoting skin cell regeneration and repair, and helping to alleviate acne-related inflammation and scarring, and can inhibit enzyme catalysis, thereby reducing tyrosinase activity and reducing pigmentation, it is also known to have poor stability and is easily oxidized, resulting in reduced biological activity. Furthermore, curcumin has poor water solubility, which can affect its penetration and absorption into the skin.

[0021] Glucomannan is a natural high molecular weight polysaccharide polymer with a large number of free hydroxyl groups in the molecule and good water solubility. Glucomannan molecules and curcumin molecules are close to each other and form a preliminary bond through intermolecular interaction. Due to the large molecular weight and entangled structure of glucomannan polysaccharide chains, it can form a dense coating layer to wrap the curcumin molecules, thereby enhancing the stability and binding force of the coating layer and protecting curcumin from environmental factors such as oxygen and light, thereby improving the stability and storage period of curcumin and enabling it to better exert its tyrosinase inhibitory effect. The hydroxyl groups and other functional groups of glucomannan may react chemically with the groups on the curcumin molecules, which can increase The polarity and hydrophilicity and lipophilicity of curcumin can improve its solubility. The free hydroxyl groups can interact with groups such as phosphate on the cell membrane, promote the entry of curcumin molecules into the cell, improve its efficiency of absorption and utilization by the cell, and facilitate the absorption and distribution of curcumin, making it easier to pass through the cell membrane and more easily interact with tyrosinase containing hydrophobic active sites, thereby improving curcumin's inhibitory ability on tyrosinase and further reducing pigmentation. By complexing glucomannan with polyacrylic acid and stearic acid, the surface modification of glucomannan can improve the coating ability of curcumin, increase the wettability and adhesion of the drug to the skin surface, improve the permeability and absorbability of curcumin, and further improve the therapeutic effect.

[0022] Preferably, the mass ratio of the polyacrylic acid, stearic acid and glucomannan is 1:2:(5-10).

[0023] By adopting the above technical solution, stearic acid and polyacrylic acid are compounded with glucomannan in the above ratio. The glucomannan surface-modified with stearic acid and polyacrylic acid can provide more active sites, which helps to more effectively coat curcumin on its surface, further improve its stability, and further reduce the instability and degradation of curcumin. In addition, the coating of curcumin by glucomannan surface modification can achieve control of drug release behavior, further improve drug durability, increase drug utilization, and further enhance therapeutic effects.

[0024] Preferably, the step S2 is: mixing the turmeric single extract and the reaction solution and stirring them uniformly, heating them for reaction and then cooling them, then adding hydroxybenzoic acid and heating and stirring them uniformly to obtain the modified turmeric single extract.

[0025] By adopting the above technical solution and introducing hydroxybenzoic acid, hydroxybenzoic acid can have a good compounding effect with stearic acid, and hydroxybenzoic acid can further increase the stability and solubility permeability of the inclusion complex, making it easier for it to pass through the cell membrane and easier to interact with tyrosinase containing a hydrophobic active site; thus, curcumin is more likely to interact with the tyrosinase active site, and this interaction can enhance the binding ability of curcumin to tyrosinase, further inhibit the activity of the enzyme, and thereby improve the therapeutic effect on acne.

[0026] Preferably, the mass ratio of stearic acid to hydroxybenzoic acid is (4-5):1.

[0027] By adopting the above technical solution and limiting the addition ratio of stearic acid and hydroxybenzoic acid within the above range, the pharmacological effect of the special medicine is further improved, the therapeutic effect on acne is improved, and pigmentation is further reduced.

[0028] Preferably, after the aqueous solution and the alcohol solution are mixed and stirred uniformly in S1, ultrasonic treatment is performed with a treatment power of 180-200 W, a treatment time of 10-15 minutes, and an interval time of 5-10 seconds.

[0029] By adopting the above technical solution and the above reaction conditions, the dissolution efficiency can be further improved and the system can be formed into a uniform dispersion system, which is beneficial to the coating effect of the effective ingredients; intermittent stirring can increase the uniformity of the system, avoid local concentrations that are too high or too low, and is beneficial to improving the quality of the filtrate; and intermittent stirring can increase the mass transfer rate in the system, promote material diffusion and exchange, and is beneficial to improving the coating efficiency.

[0030] In a second aspect, the present application provides a special medicine for treating adolescent acne, which adopts the following technical solution: a special medicine for treating adolescent acne, which is prepared by the above-mentioned preparation process of a special medicine for treating adolescent acne.

[0031] By adopting the above technical solution, the special medicine for treating adolescent acne prepared by the above preparation process has a good therapeutic effect on acne and can effectively reduce pigmentation.

[0032] In summary, this application has the following beneficial effects:

[0033] 1. Since the present application uses steam distillation to extract volatile oils from turmeric and coral ginger, the volatile components of these medicinal materials can be retained, allowing them to exert their medicinal effects in the preparation; after Gynostemma pentaphyllum, Solidago asiatica, and Paris polyphylla are crushed into fine powder, the powder is combined with the medicinal residue after oil extraction, and then soaked in 80% ethanol and slowly percolated, the active ingredients in the medicinal materials can be further extracted, thereby improving the efficacy of the preparation; the volatile oil is mixed with polysorbate 80 and then added to the medicinal solution and mixed evenly to prepare a stable emulsified system, making the preparation more stable; the obtained special medicine has the characteristics of complete extraction, stability, and long-lasting medicinal effect, further improving the therapeutic effect on acne and reducing pigmentation.

[0034] 2. In this application, glucomannan is preferably used to encapsulate the curcumin molecules, thereby enhancing the stability and binding force of the coating layer, protecting the curcumin from environmental factors such as oxygen and light, thereby improving the stability and shelf life of the curcumin, enabling it to better exert its tyrosinase inhibitory effect, and surface modification of the glucomannan by a complex of polyacrylic acid and stearic acid, thereby increasing the wettability and adhesion of the drug to the skin surface, improving the permeability and absorbability of the curcumin, and further improving the therapeutic effect.

[0035] 3. In this application, by introducing hydroxybenzoic acid, hydroxybenzoic acid can further increase the stability and solubility permeability of the inclusion complex, making it easier to pass through the cell membrane and more easily interact with tyrosinase containing hydrophobic active sites, further inhibiting the activity of the enzyme, thereby improving the therapeutic effect on acne.

[0036] 4. The special medicine for treating adolescent acne of the present application is prepared by the above-mentioned preparation process, has a good therapeutic effect on acne and can effectively reduce pigmentation. DETAILED DESCRIPTION

[0037] The present application is further described in detail below with reference to the embodiments.

[0038] Example

[0039] Example 1

[0040] A special medicine for treating adolescent acne, comprising 40g of turmeric, 38g of Paris polyphylla, 55g of Radix Angelicae Pubescentis, 20g of Schizonepeta tenuifolia, 25g of Solidago asiatica, 30g of Gynostemma pentaphyllum, and 40g of Ginger coral.

[0041] The preparation process includes the following steps:

[0042] Step 1: Grind turmeric into coarse powder, add 25 ml of distilled water to the coarse turmeric powder, and perform steam distillation at 110°C for 6 hours to obtain a turmeric single extract and turmeric residue;

[0043] Grind the coral ginger into coarse powder, add 25 ml of distilled water to the coral ginger coarse powder, perform steam distillation at 110° C. under normal pressure for 6 hours, and extract by steam distillation to obtain a coral ginger single extract and coral ginger residue;

[0044] Gynostemma pentaphyllum, Solidago asiatica, and Paris polyphylla were ground into fine powder, mixed with the above-mentioned turmeric residue and coral ginger residue, soaked in 80% ethanol, and slowly percolated, and the percolated liquid was collected;

[0045] Decoction of Radix Angelicae Dahuricae and Radix Schizonepetae with water three times, the first time for 2 hours, the second time for 1.5 hours, and the third time for 1 hour. The decoctions are combined and filtered to obtain a filtrate. The filtrate is concentrated to obtain a clear paste with a relative density of 1.18-1.22 (60° C.). Ethanol is then added to make the alcohol content reach 50%, and the paste is allowed to stand for 24 hours and filtered to obtain an alcohol precipitate. The alcohol precipitate is mixed with the percolate to obtain a mixed solution.

[0046] Step 2: 10 ml of polysorbate 80 is added to the turmeric single extract and the coral ginger single extract respectively for emulsification, and then mixed evenly with the mixed solution and filtered to obtain a special medicine for treating adolescent acne.

[0047] In this embodiment, polysorbate 80 was purchased from Xi'an Jinxiang Pharmaceutical Excipients Co., Ltd., and other raw materials were obtained from common commercial sources.

[0048] Example 2

[0049] A special medicine for treating adolescent acne, comprising 40g of turmeric, 35g of Paris polyphylla, 55g of Radix Angelicae Pubescentis, 20g of Schizonepeta tenuifolia, 25g of Solidago asiatica, 30g of Gynostemma pentaphyllum, and 40g of Ginger coral.

[0050] The preparation process includes the following steps:

[0051] Step 1: Grind turmeric into coarse powder, add 25 ml of distilled water to the coarse turmeric powder, and perform steam distillation at 110°C for 6 hours to obtain a turmeric single extract and turmeric residue;

[0052] Grind the coral ginger into coarse powder, add 25 ml of distilled water to the coral ginger coarse powder, perform steam distillation at 110° C. under normal pressure for 6 hours, and extract by steam distillation to obtain a coral ginger single extract and coral ginger residue;

[0053] Gynostemma pentaphyllum, Solidago asiatica, and Paris polyphylla were ground into fine powder, mixed with the above-mentioned turmeric residue and coral ginger residue, soaked in 80% ethanol, and slowly percolated, and the percolated liquid was collected;

[0054] Decoction of Radix Angelicae Dahuricae and Radix Schizonepetae with water three times, the first time for 2 hours, the second time for 1.5 hours, and the third time for 1 hour. The decoctions are combined and filtered to obtain a filtrate. The filtrate is concentrated to obtain a clear paste with a relative density of 1.18-1.22 (60° C.). Ethanol is then added to make the alcohol content reach 50%, and the paste is allowed to stand for 24 hours and filtered to obtain an alcohol precipitate. The alcohol precipitate is mixed with the percolate to obtain a mixed solution.

[0055] Step 2: S1, dissolving 0.5 g of glucomannan in 10 ml of water, heating to 60° C., and stirring uniformly to obtain an aqueous solution; dissolving 0.05 g of polyacrylic acid and 0.1 g of stearic acid in 10 ml of ethanol, and adding 0.05 g of sodium lauryl sulfate and stirring uniformly to obtain an alcohol solution; slowly adding the alcohol solution dropwise to the aqueous solution, stirring uniformly, mixing the aqueous solution and the alcohol solution, heating to 80° C., stirring and reacting for 1 hour, and cooling to obtain a reaction solution;

[0056] S2, mixing the turmeric single extract and the reaction solution, heating to 60°C, stirring and reacting for 5 minutes, and then ultrasonically treating for 10 minutes to obtain a modified turmeric single extract;

[0057] 10 ml of polysorbate 80 is added to the modified turmeric single extract and the coral ginger single extract respectively, and emulsified for 15 minutes, and then evenly mixed with the mixed solution to obtain a special medicine for treating adolescent acne.

[0058] In this example, glucomannan was purchased from Fufeng Sinuote Biotechnology Co., Ltd., polyacrylic acid was purchased from Shaanxi Tangyao Biotechnology Co., Ltd., and stearic acid was purchased from Guangzhou Zhanfan Technology New Materials Co., Ltd.; other raw materials were obtained from ordinary commercial sources.

[0059] Example 3

[0060] A special medicine for treating adolescent acne, which is different from Example 2 in that the raw materials include 37g of turmeric, 63g of Paris polyphylla, 37g of Radix Angelicae Pubescentis, 32g of Schizonepeta tenuifolia, 19g of Solidago asiatica, 32g of Gynostemma pentaphyllum, and 63g of Ginger coral.

[0061] Example 4

[0062] A special medicine for treating adolescent acne, which is different from Example 2 in that the raw materials include 63g of turmeric, 37g of Paris polyphylla, 63g of Radix Angelicae Pubescentis, 19g of Schizonepeta tenuifolia, 32g of Solidago asiatica, 19g of Gynostemma pentaphyllum, and 37g of Zingiber officinale.

[0063] Example 5

[0064] A special medicine for treating adolescent acne, which is different from Example 2 in that the amounts of polyacrylic acid, stearic acid and glucomannan are 0.05g, 0.1g and 0.25g respectively.

[0065] Example 6

[0066] A special medicine for treating adolescent acne, which differs from Example 2 in that, in S2: a turmeric single extract and a reaction solution are mixed and stirred evenly, and after heating reaction, 0.02 g of hydroxybenzoic acid is added and heated to 60° C., stirring is continued for 5 minutes, and then ultrasonic treatment is performed for 10 minutes to obtain a modified turmeric single extract.

[0067] In this embodiment, hydroxybenzoic acid was purchased from Guangzhou Taili Chemical Co., Ltd.

[0068] Example 7

[0069] A special medicine for treating adolescent acne, which is different from Example 6 in that the amount of hydroxybenzoic acid used is 0.025g.

[0070] Example 8

[0071] A special medicine for treating adolescent acne, which differs from Example 6 in that in S1, the alcohol solution is slowly added dropwise to the aqueous solution, and after stirring to evenly mix the aqueous solution and the alcohol solution, ultrasonic treatment is performed with a treatment power of 180 W, a treatment time of 10 minutes, and an interval time of 5 seconds. The mixture is then heated to 80° C. and stirred for reaction for 1 hour, and cooled to obtain a reaction solution.

[0072] Example 9

[0073] A special medicine for treating adolescent acne, which is different from Example 8 in that the ultrasonic treatment conditions are: treatment power of 200 W, treatment time of 15 minutes, and interval time of 10 seconds.

[0074] Example 10

[0075] A special medicine for treating adolescent acne, which differs from Example 2 in that: S1, dissolving 0.5 g of glucomannan in 10 ml of water, heating to 60° C., and stirring uniformly to obtain an aqueous solution; adding 0.05 g of sodium lauryl sulfate to 10 ml of ethanol and stirring uniformly to obtain an alcoholic solution; slowly adding the alcoholic solution dropwise to the aqueous solution and stirring uniformly; mixing the aqueous solution and the alcoholic solution, heating to 80° C., stirring for 1 hour, and cooling to obtain a reaction solution;

[0076] S2, mixing the turmeric single extract and the reaction solution, heating to 60° C., stirring and reacting for 5 minutes, and then ultrasonically treating for 10 minutes to obtain a modified turmeric single extract.

[0077] Example 11

[0078] A special medicine for treating adolescent acne, which differs from Example 2 in that: S1, dissolving 0.5 g of glucomannan in 10 ml of water, heating to 60° C., and stirring uniformly to obtain a reaction solution;

[0079] S2, mixing the turmeric single extract and the reaction solution, heating to 60° C., stirring and reacting for 5 minutes, and then ultrasonically treating for 10 minutes to obtain a modified turmeric single extract.

[0080] Performance testing

[0081] 1. In vitro transdermal absorption performance test

[0082] A Franz diffusion cell was used, with isolated rat skin as the permeation barrier, and the drugs prepared in Examples 1-9 and Comparative Examples 1-2 were used as test samples to determine the cumulative permeation of curcumin in the drugs. The specific experimental protocol is as follows: a. Preparation of isolated rat skin: Male SD rats weighing 160-220 g were used. After anesthesia, the abdominal hair was shaved and the rats were killed. The abdominal skin was quickly peeled off and subcutaneous fat and adhesions were removed. The rats were repeatedly rinsed with saline and cut into 5×5 cm pieces. 2 Soak the square in physiological saline and store for later use;

[0083] b. The ex vivo rat skin was fixed between the donor and receiving chambers with the stratum corneum facing the donor chamber. Physiological saline (5 mL) was added to the receiving chamber and stirred with a magnetic stirrer at a speed of 600 rpm. The temperature was maintained at 37°C. The effective diffusion area of the diffusion cell was 3.14 cm. 2 ;

[0084] c. Perform a transdermal experiment by applying different test samples (0.5 g) to the skin surface in the donor chamber. Take out 0.5 mL of the receiving solution at 1 h, 2 h, 4 h, 6 h, 8 h, 10 h, and 24 h, respectively, and make up the volume with normal saline. This experiment is continued for 24 h. The curcumin content in the receiving solution is determined by HPLC, and the permeation amount of curcumin is calculated and recorded in Table 2.

[0085] d. Skin retention test: After the transdermal test, the skin was removed from the diffusion cell, the surface was washed with physiological saline, and the skin was cut into small pieces with scissors. The pieces were soaked in ethanol (10 mL) for 24 h. The curcumin content in ethanol was determined by HPLC, and the skin retention amount was calculated and recorded in Table 2.

[0086] The chromatographic conditions for the determination of curcumin content by HPLC were as follows: a C18 column (4.6×250 mm, 5 μm), acetonitrile-water (50:50, v / v) as the mobile phase, a flow rate of 1 mL / min, a detection wavelength of 425 nm, a column temperature of 25°C, and an injection volume of 20 μL.

[0087] 2. Tyrosinase Inhibition Ability Assay Prepare reagents: Weigh tyrosinase and dilute it with PBS to a concentration of 150 U / mL; PBS phosphate buffer solution is 0.2 M, pH = 6.8; the test solutions are the special drugs prepared in the examples and comparative examples respectively; weigh an appropriate amount of L-Dopa and dilute it with PBS to a concentration of 1.5 mg / mL.

[0088] Experimental procedure: Add different combinations of reagents into a test tube according to the ratio in Table 1. First, place it in a 35°C water bath for 10 minutes, then add tyrosinase solution, mix well, and incubate at 35°C for 30 minutes. Then, quickly transfer it to a cuvette and measure the absorbance at 475nm.

[0089] Calculation of inhibition rate: Based on the absorbance values of different groups, the inhibition rate of tyrosinase was calculated using the formula: Inhibition rate = [(A1-A2)-(B1-B2)] / (A1-A2)×100%, and recorded in Table 2; where B1 is the test solution group, A1 is the control group 1, A2 is the control group 2, and B2 is the absorbance value of the control group 3.

[0090] Table 1

[0091]

[0092] Test data:

[0093] Table 3

[0094]

[0095]

[0096] In combination with Examples 1-2 and Table 3, it can be seen that the cumulative permeation amount and skin retention amount of curcumin in the special medicine prepared in Example 2 are significantly better than the cumulative permeation amount and skin retention amount of curcumin in the special medicine prepared in Example 1, and the inhibition rate of tyrosinase is significantly improved, indicating that by pre-treating the turmeric single extract with glucomannan, polyacrylic acid and stearic acid, the transdermal absorption performance of curcumin can be effectively improved, thereby improving the therapeutic effect on acne, and effectively inhibiting the activity of tyrosinase, thereby reducing pigmentation.

[0097] In combination with Example 2 and Examples 10-11, and in combination with Table 3, it can be seen that the special medicine prepared in Example 2, the cumulative permeation amount and the skin retention amount of curcumin are significantly better than the cumulative permeation amount and the skin retention amount of curcumin prepared in Examples 10 and 11, and the special medicine prepared in Example 2 has a significantly improved inhibition rate on tyrosinase, indicating that when the turmeric single extract is pretreated, the surface modification of glucomannan by a complex of polyacrylic acid and stearic acid can further improve the transdermal absorption performance of curcumin.

[0098] In combination with Examples 2-9, and in combination with Table 3, it can be seen that when the turmeric single extract is pretreated, the optimization of the ratio of the pretreatment raw materials and the optimization of the treatment process can effectively improve the transdermal absorption performance of curcumin, further improve the therapeutic effect of the special medicine on acne, and further reduce pigmentation; In combination with Example 2 and Examples 6-7, it can be seen that the special medicine prepared in Example 6-7 has a cumulative permeation amount and a skin retention amount of curcumin that are significantly better than the cumulative permeation amount and the skin retention amount of the special medicine prepared in Example 2. This shows that after the turmeric single extract is pretreated with glucomannan and then modified with hydroxybenzoic acid, the inhibitory ability of curcumin on tyrosinase can be further improved, and the transdermal absorption performance of curcumin can be further improved.

[0099] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make non-creative modifications to the present embodiment as needed, but as long as they are within the scope of the claims of the present application, they are protected by the patent law.

Claims

1. A method for preparing a special medicine for treating adolescent acne, characterized in that: The Chinese medicinal materials are 40g of turmeric, 35g of Paris polyphylla, 55g of Radix Angelicae Pubescentis, 20g of Schizonepeta tenuifolia, 25g of Solidago asiatica, 30g of Gynostemma pentaphyllum, and 40g of Ginger coral. The preparation process includes the following steps: Step 1: Grind turmeric into coarse powder, add 25 ml of distilled water to the coarse turmeric powder, and perform steam distillation at 110°C for 6 hours to obtain a turmeric single extract and turmeric residue; Grind the coral ginger into coarse powder, add 25 ml of distilled water to the coral ginger coarse powder, perform steam distillation at 110° C. under normal pressure for 6 hours, and extract by steam distillation to obtain a coral ginger single extract and coral ginger residue; Gynostemma pentaphyllum, Solidago asiatica, and Paris polyphylla were ground into fine powder, mixed with the above-mentioned turmeric residue and coral ginger residue, soaked in 80% ethanol, and slowly percolated, and the percolate was collected; Decoction of Radix Angelicae Dahuricae and Herba Schizonepetae with water for three times, the first time for 2 hours, the second time for 1.5 hours, and the third time for 1 hour. The decoctions are combined and filtered to obtain a filtrate. The filtrate is concentrated to obtain a clear paste having a relative density of 1.18-1.22 at 60° C. Ethanol is then added to make the alcohol content reach 50%, and the mixture is allowed to stand for 24 hours and filtered to obtain an alcohol precipitate. The alcohol precipitate is mixed with the percolate to obtain a mixed solution. Step 2: S1, dissolving 0.5 g of glucomannan in 10 ml of water, heating to 60° C., and stirring uniformly to obtain an aqueous solution; dissolving 0.05 g of polyacrylic acid and 0.1 g of stearic acid in 10 ml of ethanol, and adding 0.05 g of sodium lauryl sulfate and stirring uniformly to obtain an alcohol solution; slowly adding the alcohol solution dropwise to the aqueous solution, stirring uniformly, mixing the aqueous solution and the alcohol solution, heating to 80° C., stirring and reacting for 1 hour, and cooling to obtain a reaction solution; S2, mixing the turmeric single extract and the reaction solution, heating to 60°C, stirring and reacting for 5 minutes, and then ultrasonically treating for 10 minutes to obtain a modified turmeric single extract; 10 ml of polysorbate 80 is added to the modified turmeric single extract and the coral ginger single extract respectively, and emulsified for 15 minutes, and then evenly mixed with the mixed solution to obtain a special medicine for treating adolescent acne.

2. The method for preparing a special medicine for treating adolescent acne according to claim 1, characterized in that: S2: mixing the turmeric single extract and the reaction solution and stirring them uniformly, heating for reaction and then cooling, then adding 0.02g or 0.025g of hydroxybenzoic acid and heating and stirring them uniformly to obtain a modified turmeric single extract.

3. The method for preparing a special medicine for treating adolescent acne according to claim 1, characterized in that: In S1, the aqueous solution and the alcohol solution are mixed and stirred uniformly, and then ultrasonic treatment is performed with a treatment power of 180-200 W, a treatment time of 10-15 minutes, and an interval time of 5-10 seconds.

4. A special medicine for treating adolescent acne prepared according to the method for preparing a special medicine for treating adolescent acne according to any one of claims 1 to 3.

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