Iron-carbon particles, methods of making, and shewanella-containing iron-carbon particles

Abstract

The application discloses an iron-carbon particle, a preparation method and an iron-carbon particle containing shewanella, and relates to municipal sludge preliminary processing to form a precursor of the iron-carbon particle, acid pickling modification of the precursor of the iron-carbon particle to obtain a porous iron-carbon particle, and soaking of the iron-carbon particle in a shewanella solution to obtain the iron-carbon particle containing shewanella. Micro-electrolysis of the iron-carbon particle can promote the degradation capacity of shewanella to pollutants, and the microbial degradation of shewanella and the adsorption and degradation effect of the iron-carbon particle can further improve the removal effect of antibiotics and heavy metals. The production cost is low, the production efficiency is high, and batch production is facilitated.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of aquaculture, and particularly relates to an iron-carbon particle, a preparation method and an iron-carbon particle containing shewanella. BACKGROUND

[0002] Currently, antibiotics commonly used in the aquaculture industry mainly include amoxicillin, enrofloxacin, erythromycin, florfenicol, sulfadiazine and sulfamethoxazole, etc. Enrofloxacin is the first animal-specific antibiotic among quinolone antibacterial drugs. Due to its wide antibacterial spectrum, strong antibacterial activity, no cross-resistance with commonly used antibacterial drugs and other characteristics, it has become the most widely used drug in the process of disease prevention and treatment in aquaculture. The excessive use of these antibiotic substances for a long time causes excessive deposition of antibiotics and heavy metals in the tail water and sediment of aquaculture, which will cause secondary pollution to the aquatic organisms and the environment for a long time. The abuse of antibiotics will induce biological drug resistance, thus producing super bacteria and antibiotic resistance genes, making the tail water treatment of aquaculture difficult. On the one hand, the antibiotics re-enter the water body and are absorbed by aquatic organisms, thereby affecting human health, on the other hand, the antibiotics can directly kill or inhibit the growth of beneficial microorganisms that degrade the sediment in aquaculture, thereby destroying the structure and function of the microbial community, and increasing the harm to the environment and human health; therefore, it is necessary to treat the antibiotics and heavy metals in the tail water of aquaculture.

[0003] Among them, the "three-pool two-dam" tail water treatment system has been widely applied in the field of aquaculture, but the traditional "three-pool two-dam" treatment process has good treatment effect on suspended solids, particulate matter, nitrogen, phosphorus and other organic substances in the tail water of aquaculture, but the overall treatment effect on antibiotics and heavy metals and other substances is not good. At present, the research on the removal of antibiotics and other refractory pollutants by the "three-pool two-dam" process focuses on the adsorption removal of traditional fillers, and there are few reports on the combined action of iron-carbon materials and microorganisms as ecological dam fillers to remove antibiotics in tail water. The existing iron-carbon materials have low catalytic activity on antibiotics and heavy metals, and the activation time of the refractory macromolecular organic matter is too long, thereby reducing the removal effect of antibiotics and heavy metals.

[0004] At present, there are methods for removing antibiotics and heavy metals by using microorganisms, but the effect of using microorganisms alone to remove antibiotics and heavy metals in water is poor, and the expected effect cannot be achieved. At the same time, the survival conditions of microorganisms in water are poor, the death rate is fast, the removal efficiency of heavy metals and antibiotics in water is low, and the water quality cannot be effectively improved. SUMMARY

[0005] The present application aims to provide an iron-carbon particle, a preparation method and a shewanella-containing iron-carbon particle, which can prepare the iron-carbon particle from municipal sludge, and make the iron-carbon micro-electrolysis and shewanella play a combined effect by combining the shewanella with the iron-carbon particle, so as to effectively remove heavy metals and antibiotics in water.

[0006] The present application is realized by the following technical solutions:

[0007] A preparation method of an iron-carbon particle, comprising the following steps:

[0008] S1: collecting municipal sludge;

[0009] S2: dewatering the municipal sludge so that the internal moisture content is between 65% and 75%, and then airing the municipal sludge;

[0010] S3: placing the air-dried municipal sludge into an oven for drying, and then placing it into a pulverizer for pulverization and sieving;

[0011] S4: placing the municipal sludge obtained after the pulverization and sieving into a hydrothermal kettle for 180℃ hydrothermal treatment for 6 h, filtering and drying to constant weight after cooling, to obtain a precursor of the iron-carbon particle;

[0012] S5: modifying the precursor of the iron-carbon particle to obtain the iron-carbon particle.

[0013] Further, the specific operation method for modifying the precursor of the iron-carbon particle to obtain the iron-carbon particle in the step S5 is that: the precursor of the iron-carbon particle is mixed with one or more of sugar, clay and manganese iron ore, and then placed into a tube furnace for re-firing, the heating rate is controlled at 10℃ / min and the temperature is raised to 800℃, nitrogen is added for pyrolysis for 2h, the surface dust is washed with deionized water after cooling, dried to constant weight and sieved, and finally granulated and dried using a granulator to obtain the iron-carbon particle.

[0014] Further, the temperature of the oven in the step S3 is between 200℃ and 220℃, and the drying time is 2h.

[0015] An iron-carbon particle, which is prepared by the preparation method of the iron-carbon particle according to any one of the above.

[0016] A shewanella-containing iron-carbon particle, which is obtained by combining the above-mentioned iron-carbon particle with shewanella, and specifically comprising the following steps:

[0017] D1: preparing the iron-carbon particle for standby;

[0018] D2: preparing a shewanella solution;

[0019] D3: Soaking the iron-carbon particles in the shewanella solution;

[0020] D4: Drying the iron-carbon particles from the shewanella solution.

[0021] Further, the specific method for preparing the shewanella solution in step D2 is: stirring the shewanella biological inoculant with sterile water at a ratio of 1:5-10 until dissolution, and adding a certain proportion of a survival agent to the shewanella solution to increase the survival rate of shewanella.

[0022] Further, the composition of the survival agent includes one or more of peptone, yeast powder, glucose, inorganic salt, vitamin, nitrate, chloride, and bromide.

[0023] Further, when preparing the shewanella solution, the temperature of the sterile water is controlled at 20-25°C, and the pH value is controlled at 7.4-7.8.

[0024] Further, when preparing the shewanella solution, the culture time of the shewanella solution is 24-48h, so that the culture concentration reaches 10 9 mg / L, and then a magnetic stirrer is used for continuous stirring for 2-3h.

[0025] Compared with the prior art, the present application has the following advantages and beneficial effects:

[0026] 1) In the present application, municipal sludge can be used as the raw material of the iron-carbon particles. After collecting, drying, and airing the municipal sludge, an iron-carbon particle precursor is obtained. After adding auxiliary materials to the iron-carbon particle precursor for modification, drying, crushing, and sieving again, and finally granulating by a granulator, the iron-carbon particles are obtained. Then, the iron-carbon particles are soaked in the shewanella solution to obtain the finished iron-carbon particles. The production cost is low, the production efficiency is high, and batch production is convenient.

[0027] 2) In the present application, the ecological filter dam is added with the iron-carbon particles to filter the aquaculture tail water. Through the combination of the iron-carbon particles and shewanella, the iron-carbon particles play the role of iron-carbon micro-electrolysis, and the shewanella plays the role of decomposition, so as to decompose and remove the antibiotics and heavy metals in the aquaculture tail water. The iron-carbon micro-electrolysis can promote the degradation ability of shewanella to pollutants, and the microbial degradation of shewanella and the adsorption and degradation of the iron-carbon particles can further improve the removal effect of antibiotics and heavy metals. Thus, the decomposition efficiency of heavy metals and antibiotics in water can be increased, and the water purification effect can be enhanced. BRIEF DESCRIPTION OF DRAWINGS

[0028] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings in the embodiments will be briefly introduced as follows. It should be understood that the following drawings only show some of the embodiments of the present application, and therefore should not be regarded as a limitation on the scope. Other related drawings can also be obtained by those of ordinary skill in the art without creative labor on the basis of these drawings.

[0029] Figure 1 Figure for the removal effect of antibiotics and heavy metals by the combination of iron-carbon particles and shewanella in the present application.

[0030] Figure 2 Figure for the removal effect of antibiotics of different concentrations by the iron-carbon particles prepared from municipal sludge in the present application.

[0031] Figure 3 Figure for the removal effect of antibiotics and heavy metals by shewanella in the present application.

[0032] Figure 4 Figure for the comparison of the precursor of iron-carbon particles before and after modification in the present application. DETAILED DESCRIPTION

[0033] In order to make the purpose, technical solutions and advantages of the embodiments of the present application more clear, the technical solutions in the embodiments of the present application will be described clearly and completely in combination with the drawings in the embodiments of the present application. Obviously, the described embodiments are some of the embodiments of the present application, but not all the embodiments.

[0034] As shown in Figure 1 , Figure 2 and Figure 3 , iron-carbon micro-electrolysis is a proven efficient and low-cost technology for treating various pollutant-containing wastewater, including heavy metals, nitrate, antibiotics and other pollutants, etc. It mainly uses iron as anode and carbon as cathode, many micro-current cells are spontaneously formed, and a series of physical and chemical reactions are generated, and the main mechanisms for removing pollutants include adsorption, oxidation and reduction, active oxygen degradation, and co-precipitation, etc.

[0035] Shewanella is a kind of dissimilatory iron-reducing bacteria, which is a facultative anaerobic bacteria. It can use a variety of substances as electron acceptors. At the beginning, it was widely used in the remediation of chromium, iron and other heavy metals. However, with the further development of research, it was found that shewanella can also use organic pollutants such as o-xylene and pyridine as electron acceptors, so it can also have good removal effect on antibiotics and other organic substances.

[0036] As can be seen from Figure 1 , the combination of iron-carbon particles and shewanella can effectively and quickly remove heavy metals and antibiotics in tail water, and the efficiency of removing heavy metals is higher than that of removing antibiotics.

[0037] from Figure 2 The results show that the iron-carbon particles prepared from municipal sludge effectively removed antibiotics at concentrations of 5 Mg / L, 3 Mg / L, and 1 Mg / L from the effluent.

[0038] from Figure 3 The results show that Shewanella is effective in removing antibiotics and heavy metals.

[0039] Example 1:

[0040] This embodiment uses municipal sludge to prepare iron-carbon particles, such as Figure 4 As shown, the specific method for collecting raw materials and performing preliminary processing to form iron-carbon particles includes:

[0041] S1: Collect municipal sludge;

[0042] S2: Dewater the municipal sludge to a moisture content of 65%-75%, and then dry the municipal sludge.

[0043] S3: After drying, the municipal sludge is placed in an oven to dry, and then put into a pulverizer for crushing and sieving;

[0044] S4: The municipal sludge obtained after crushing and sieving is placed in a hydrothermal reactor and subjected to hydrothermal treatment at 180℃ for 6 hours. After cooling, it is filtered and dried to constant weight to obtain the precursor of iron-carbon particles.

[0045] S5: Modify the precursor of iron-carbon particles to obtain iron-carbon particles.

[0046] The specific operation method for processing the precursor of iron-carbon particles in step S5 to obtain iron-carbon particles is as follows: the precursor of iron-carbon particles is thoroughly mixed with one or more of white sugar, clay, and manganese iron ore, and then placed in a tube-type resistance furnace for firing again. The heating rate is controlled at 10 ℃ / min and the temperature is raised to 800 ℃. Nitrogen is added to pyrolyze it for 2 hours. After cooling, the surface dust is washed off with deionized water, dried to constant weight, and sieved. Finally, granulation is performed using a granulator and then dried to obtain iron-carbon particles.

[0047] In step S13, the temperature of the drying oven is between 200℃ and 220℃, and the drying time is 2 hours.

[0048] When municipal sludge is used as the raw material of the iron-carbon particles, the municipal sludge is the sludge after dewatering treatment in a sewage treatment plant, and the water content is about 70%. The municipal sludge is dried to further remove the water content, and then the dried municipal sludge is placed in an oven for drying treatment. The temperature is set to 210°C for 2h. The dried municipal sludge is ground by a grinder and passed through a 100 mesh screen. The ground and screened municipal sludge is placed in an autoclave for hydrothermal treatment at 180°C for 6h. After cooling, the mixture is collected by filtration and dried to constant weight to obtain the precursor of the iron-carbon particles.

[0049] The precursor of the iron-carbon particles is modified. The precursor of the iron-carbon particles is mixed with white sugar, clay, and manganese ore, and then placed in a tube furnace for sintering again. The temperature is raised to 800°C at a rate of 10°C / min, and pyrolysis is performed in a nitrogen atmosphere for 2h. After cooling to room temperature, the surface dust is washed with deionized water, dried to constant weight, and sieved through a 100 mesh screen for dry storage.

[0050] The municipal sludge contains many metal substances, and the precursor of the iron-carbon particles contains a large amount of iron oxide. The content of iron oxide in the municipal sludge is relatively high and the price is low. The addition of white sugar and clay is to enhance the adhesion between the raw materials during granulation. White sugar can increase the porosity of the iron-carbon particles and increase their surface area, thereby increasing the loading rate of Shewanella and enhancing the adsorption performance of the iron-carbon particles.

[0051] The sintered precursor of the iron-carbon particles is placed in a granulator for granulation treatment to prepare ceramsite with a particle size of 25-40mm. The prepared ceramsite is dried to constant weight in an oven at 105°C. Then the ceramsite is taken out to form iron-carbon particles, which are stored in a vacuum drying box for standby use.

[0052] Example 2:

[0053] The precursor of the iron-carbon particles can also use existing iron-containing ceramsite. The iron-containing ceramsite is modified to form iron-carbon particles.

[0054] The modified iron-carbon particles using iron-carbon-containing ceramsite only need to be acid washed to obtain iron-carbon particles. After modification, Shewanella can be loaded, which is convenient and fast.

[0055] Hydrochloric acid and nitric acid solution is added to water in a certain proportion, the volume ratio of the prepared is: strengthening agent hydrochloric acid: strengthening agent nitric acid: water = 1:1:8; a certain volume of ceramsite is put into a conical flask, a certain volume of acid solution is added, the ratio between ceramsite and acid solution is 1:3, then mix and shake well, place in a shaking water tank, take out after 24h reaction. The modified ceramsite is washed with clean water, and finally placed in a 105℃ oven to dry to constant weight, forming iron-carbon particles, and finally the iron-carbon particles are placed in a vacuum drying oven for storage.

[0056] Iron-carbon particles can be obtained by modifying the above two raw materials, the cost of preparing iron-carbon particles using municipal sludge is relatively low, and the speed of preparing iron-carbon particles using existing iron-containing ceramsite is faster and the process is simple.

[0057] Example 3:

[0058] An iron-carbon particle containing shewanella, the iron-carbon particle is combined with shewanella to obtain an iron-carbon particle containing shewanella, which specifically includes the following steps:

[0059] D1: Prepare iron-carbon particles for standby;

[0060] D2: Prepare shewanella solution;

[0061] D3: Soak the iron-carbon particles in the shewanella solution;

[0062] D4: Dry the iron-carbon particles from the shewanella solution.

[0063] The specific method for preparing the shewanella solution in step D2 is: stirring the shewanella biological inoculant with sterile water in a ratio of 1:5-10 until dissolved, and adding a certain proportion of a survival agent to the shewanella solution to increase the survival rate of shewanella;

[0064] The composition of the survival agent includes one or more of peptone, yeast powder, glucose, inorganic salt, vitamin, nitrate, chloride, bromide.

[0065] When preparing the shewanella solution, the temperature of the sterile water is controlled at 20-25℃, the pH value is controlled at 7.4-7.8, the culture time of the shewanella solution is 24-48h, and after the culture concentration reaches 109mg / L, the magnetic stirrer is used for continuous stirring for 2-3h.

[0066] The shewanella is a shewanella biological inoculant purchased, which needs to be cultured before inoculation and use.

[0067] The purchased Shewanella bio-inoculant is stirred with sterile water in a ratio of 1:5-10 until dissolved, 100-200g of bio-inoculant is used per 1kg of ceramsite, and some nutrients required for growth need to be added to the culture medium, the main components of which include peptone 5.0g / L, yeast powder 1.0g / L, glucose, and various inorganic salts and vitamins, and some nitrate, chloride, bromide and other trace elements can also be added. To ensure the survival rate of Shewanella, a higher concentration solution is used as much as possible, and the culture conditions need to be strictly controlled during the culture, the culture temperature is controlled at 20°C, the pH value is controlled between 7.6±0.2, the culture time is 24-48h, and when the culture concentration reaches 109mg / L, the glucose solution is added after stirring with sterile water for 30min, and the magnetic stirrer is continuously stirred for 2-3h, and the Shewanella bio-inoculant solution is obtained after stirring.

[0068] The prepared iron-carbon particles are added to the prepared Shewanella solution, and the iron-carbon particles should be kept in a submerged state during addition, and then fully stirred to ensure that the iron-carbon particles and the Shewanella solution are fully contacted. Then the iron-carbon particles are soaked in the Shewanella solution for 24 hours, and stirring is performed in time to ensure the survival rate and loading rate of Shewanella.

[0069] During the soaking and solution preparation, the pH, temperature, dissolved oxygen, trace elements, salinity and concentration of toxic substances of the solution should be maintained to prevent other conditions from causing Shewanella to die and affect the survival rate and loading rate of Shewanella.

[0070] After 24 hours, the submerged iron-carbon particles are taken out and air-dried to obtain iron-carbon particles containing Shewanella. During the period, the temperature is strictly controlled and checked in time. The other parts of this embodiment are the same as the above-mentioned embodiments, which will not be repeated here.

[0071] The iron-carbon particles can purify the sewage by iron-carbon micro-electrolysis. The iron-carbon micro-electrolysis is to use iron filings and carbon to form a primary cell. The metal material and activated carbon are put into the electrolyte solution at the same time, and a large number of micro primary cells are formed. Therefore, the iron-carbon micro-electrolysis method is a treatment method integrating oxidation-reduction, adsorption, flocculation, electrodeposition and co-precipitation. It can remove part of the refractory organic matter, and at the same time, it can change the structure and form of the refractory organic matter, so as to remove it. In the process of treating the tail water of aquaculture, when the water flows through the dam body, the iron-carbon particles intercept and adsorb the antibiotics and heavy metals through the pores on the surface layer, and then the ceramsite in the dam body acts. The trivalent iron ions in the iron-carbon material will rapidly hydrolyze to generate iron-containing hydroxyl oxide. Under the condition of acid and rich oxygen, the oxygen will produce a stronger electrode potential, thereby increasing the potential difference of micro-electrolysis and accelerating the oxidation effect of iron molecules in the iron-carbon particles, so that the antibiotics in the water body are fully degraded under the action of oxidation-reduction, electric coagulation, flocculation and adsorption.

[0072] There are two aspects of the degradation of organic pollutants by Shewanella. The first is the degradation of organic pollutants by single Shewanella, which mainly uses organic pollutants such as azo dyes and nitroaromatic compounds as electron acceptors, and uses extracellular enzymes and intracellular oxidoreductases to diffuse and degrade the pollutants. The second is the degradation of organic pollutants by Shewanella driven Fenton reaction. Shewanella can use Fe(III) and O2 as electron acceptors in anaerobic and micro-aerobic environments, respectively, to produce Fe(II) and H2O2 to drive Fenton reaction, and to produce reactive oxygen and hydroxyl radicals to realize the degradation of various organic pollutants. In the tail water treatment system of aquaculture, the continuous flow of water and the interception effect of the filter dam can provide good and anaerobic treatment conditions for the water during the treatment process, and the continuous water body provides a rich carbon source for the growth of these microorganisms, so that a complete Fenton oxidation system can be formed in the filter dam, thereby promoting the degradation of antibiotics and heavy metals and other substances in the water body.

[0073] The iron-carbon particles provide good attachment space for the growth of shewanella, and the iron-carbon particles can also have good adsorption and degradation effect on pollutants, and shewanella can form a biological battery by utilizing heavy metals and other pollutants in the water, and the iron-carbon particles also rely on the principle of iron-carbon micro-electrolysis to realize the removal of antibiotics, and the interaction between shewanella and iron-carbon particles enhances the effect of the original battery, thereby increasing the electrode potential of O2 in the water body and the potential difference required for micro-electrolysis, and shewanella can also drive the Fenton oxidation reaction to produce more hydroxyl radicals to remove antibiotic substances. The effect of the interaction and mutual influence of the iron-carbon particles and shewanella makes them achieve better removal effect in the process of removing aquaculture tail water, and the removal rate of antibiotics is increased by about 20% compared with the effect of using shewanella and iron-carbon particles alone to degrade antibiotic tail water.

[0074] In the description of the present application, it should be pointed out that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship when the product of the present application is usually placed, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.

[0075] In addition, the terms "horizontal", "vertical" and the like in the description of the present application do not mean that the components must be absolutely horizontal or vertical, but can be slightly inclined. For example, "horizontal" only means that its direction is more horizontal relative to "vertical", and does not mean that the structure must be completely horizontal, but can be slightly inclined.

[0076] In the description of the present application, it should be pointed out that, unless otherwise explicitly specified and limited, if the terms "arrangement", "installation", "connection", "connection" appear, they should be understood in a broad sense, for example, they can be fixedly connected, or detachably connected, or integrally connected; can be mechanically connected, or electrically connected; can be directly connected, or indirectly connected through an intermediate medium; can be connected inside two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0077] The above is only the preferred embodiment of the present application, and does not limit the present application in any form. Any simple modification or equivalent change made according to the technical essence of the present application to the above embodiment falls within the protection scope of the present application.

Claims

1. A method of producing a Shewanella ferric carbonate particle, comprising the steps of, The iron-carbon particles are combined with Shewanella bacteria to obtain iron-carbon particles containing Shewanella bacteria, specifically including the following steps: D1: Prepare iron-carbon granules for later use; D2: Preparation of Shewanella solution; D3: Soak the iron-carbon particles in Shewanella solution for 24 hours, stirring regularly during the soaking period to ensure the survival rate and colonization rate of Shewanella. D4: Remove the iron-carbon particles from the Shewanella solution and dry them; The preparation process of iron-carbon particles includes the following steps: S1: Collect municipal sludge; S2: Dewater the municipal sludge to a moisture content of 65%-75%, and then dry the municipal sludge. S3: After drying, the municipal sludge is placed in an oven to dry, and then put into a pulverizer for crushing and sieving; S4: The municipal sludge obtained after crushing and sieving is placed in a hydrothermal reactor and subjected to hydrothermal treatment at 180℃ for 6 hours. After cooling, it is filtered and dried to constant weight to obtain the precursor of iron-carbon particles. S5: Modify the precursor of iron-carbon particles to obtain iron-carbon particles; during the modification process, the precursor of iron-carbon particles is fully mixed with white sugar, clay and manganese iron ore and then placed in a tube resistance furnace for firing, followed by pyrolysis and granulation; the fired precursor of iron-carbon particles is placed in a granulator for granulation to prepare ceramsite with a particle size between 25mm and 40mm, the prepared ceramsite is dried in an oven at 105℃ until constant weight, and then the ceramsite is taken out to form iron-carbon particles.

2. The preparation method of Shewanella iron-carbon particles as described in claim 1, characterized in that, In step S5, the specific operation method for modifying the precursor of iron-carbon particles to obtain iron-carbon particles is as follows: the precursor of iron-carbon particles is thoroughly mixed with white sugar, clay and manganese iron ore and then put into a tubular resistance furnace. The heating rate of the tubular resistance furnace is controlled at 10℃ / min and the temperature is raised to 800℃. Nitrogen gas is added to pyrolyze it for 2 hours. After cooling, the surface dust is washed off with deionized water, dried to constant weight and sieved. Finally, granulation is performed using a granulator and then dried to obtain iron-carbon particles.

3. The preparation method of Shewanella iron-carbon particles as described in claim 1, characterized in that, In step S3, the temperature of the drying oven is between 200℃ and 220℃, and the drying time is 2 hours.

4. The preparation method of Shewanella iron-carbon particles as described in claim 1, characterized in that, The specific method for preparing Shewanella solution in step D2 is as follows: Shewanella biological agent and sterile water are stirred in a ratio of 1:5-10 until dissolved, and a certain proportion of viability agent is added to the Shewanella solution to increase the survival rate of Shewanella.

5. The preparation method of Shewanella iron-carbon particles as described in claim 4, characterized in that, The active ingredient comprises one or more of the following: peptone, yeast powder, glucose, inorganic salts, vitamins, nitrates, chlorides, and bromides.

6. The preparation method of Shewanella iron-carbon particles as described in claim 1, characterized in that, When preparing the Shewanella solution, the temperature of the sterile water is controlled at 20℃-25℃, and the pH value is controlled between 7.4 and 7.

8.

7. The method for preparing Shewanella iron-carbon particles as described in claim 1, characterized in that, The Shewanella solution is prepared by culturing the Shewanella for 24-48 hours to reach a concentration of 10 9 mg / L, and then continuously stirring the solution for 2-3 hours using a magnetic stirrer.

8. An iron-carbon granule containing Shewanella bacteria, characterized in that, Iron-carbon particles prepared by the method for preparing Shewanella iron-carbon particles according to any one of claims 1-7.

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