A radiation protection composition containing natural fungus extract and its application

Abstract

The present invention discloses a radiation protection composition including a medicinal pore fungus extract, and relates to the technical field of compositions. The present invention provides a radiation protection composition, comprising the following components: an active substance and a polysaccharide, wherein the weight ratio of the active substance to the polysaccharide is (0.1-5): (3-10); the active substance comprises at least one of quercetin and oxidized resveratrol; the polysaccharide comprises a first polysaccharide and a second polysaccharide, wherein the first polysaccharide is a medicinal pore fungus extract, and the second polysaccharide comprises at least one of yeast glucan and schizophyllan; the weight ratio of the first polysaccharide to the second polysaccharide is (1-10): (0.01-2). The present invention achieves excellent radiation protection, whitening, oil control, and repairing effects through the composite use of specifically selected active substances and polysaccharides, and is mild and non-irritating.

Description

Technical Field

[0001] The invention relates to the technical field of compositions, in particular to a radiation protection composition containing a medicinal porphyra extract and application thereof. Background Art

[0002] Radioactive substances can spread in the air and harm the human body through skin contact. Nuclear radiation mainly includes alpha, beta and gamma radiation, but the damage to organisms is mainly caused by gamma rays. Alpha rays and beta rays have longer wavelengths and less penetrating power. Gamma rays are a type of high-energy electromagnetic wave with a very short wavelength and strong penetrating power, which is the most harmful to the human body. Gamma radiation can ionize water to produce a large number of highly active and unstable reactive oxygen free radicals, causing cells to produce oxidative stress reactions and leading to cell dysfunction. High-energy radiation can also directly destroy the DNA structure of organisms, causing DNA chain breaks and causing irreversible genetic damage.

[0003] Common nuclear radiation protection methods include using radiation shielding building materials to isolate radiation sources, using protective equipment such as radiation protection clothing and radiation masks, and using nuclear radiation prevention and treatment drugs such as aminothiol, vitamins, flavonoids and polysaccharides. The disadvantages of these two methods are: most nuclear radiation protection equipment is designed for high-radiation environments. Although it has strong protection capabilities, it is heavy and not portable, and is not convenient for daily use; taking nuclear radiation prevention and treatment drugs can repair radiation damage, but it does not have the effect of blocking nuclear radiation from the source.

[0004] There is no skin product with radiation resistance function in the market. Therefore, a skin product with radiation resistance function has broad application prospects. Summary of the invention

[0005] Based on this, the purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide a radiation protection composition containing a medicinal porphyra extract and its application.

[0006] To achieve the above object, the technical solution adopted by the present invention is as follows: the present invention provides a radiation protection composition comprising a medicinal porphyra extract.

[0007] The invention also provides application of the medicinal porphyra extract in preparing a radiation protection composition.

[0008] The present invention provides a radiation protection composition, comprising the following components: an active substance and a polysaccharide, wherein the weight ratio of the active substance to the polysaccharide is (0.1-5):(3-10); the active substance comprises at least one of quercetin and oxidized resveratrol; the polysaccharide comprises a first polysaccharide and a second polysaccharide, wherein the first polysaccharide is a medicinal porphyra extract, and the second polysaccharide comprises at least one of yeast glucan and schizophyllan; the weight ratio of the first polysaccharide to the second polysaccharide is (1-10):(0.01-2).

[0009] The inventors found that the medicinal pore fungus extract itself is a complex polysaccharide with a molecular weight of 80-90kDa and 190-210kDa. The medicinal pore fungus extract itself has two different ranges of molecular weight, which can absorb the energy of low-dose γ radiation and high-dose γ radiation, achieving a full range of radiation protection. Preferably, the medicinal pore fungus is rich in Alihong polysaccharide, which consists of a 199kDa macromolecular polysaccharide component and an 87kDa small molecule polysaccharide component, and can absorb different doses of γ radiation energy to achieve a full range of radiation protection. In addition, Alihong polysaccharide can also inhibit the migration of white blood cells from capillaries to inflammatory sites, reduce inflammatory reactions, and inhibit the growth of acne bacteria, playing an anti-acne and anti-allergic effect. It can also protect cells from damage by improving the skin barrier, fighting light radiation and oxidative free radicals.

[0010] The inventors of the present application found in the actual experimental process that the combination of specifically selected polyphenol actives and polysaccharides can achieve better radiation protection, whitening and oil control effects. Polyphenol actives have a stable structure of benzene rings and active phenolic hydroxyl groups, which have the function of blocking and absorbing gamma radiation. Polysaccharides can absorb the energy of gamma radiation and degrade into oligosaccharides or monosaccharides, thereby exposing more active sites and increasing radiation protection effects and other biological activities.

[0011] The radiation protection composition of the specifically selected polyphenol active substance and polysaccharide provided by the present invention is 60 Gamma ray irradiation produced by Co source, with irradiation dose of 1-9kGy.

[0012] Preferably, the weight ratio of the polyphenol active ingredient to the polysaccharide is (0.5-5):(4-10), and more preferably, the weight ratio of the polyphenol active ingredient to the polysaccharide is (2-3):(7-8).

[0013] Preferably, the weight ratio of the first polysaccharide to the second polysaccharide is (10-40):1.

[0014] Preferably, the polyphenol active ingredient is a mixture of quercetin and oxidized resveratrol, and the weight ratio of the quercetin to the oxidized resveratrol is (0.05-0.2):(0.5-5); preferably, the weight ratio of the quercetin to the oxidized resveratrol is (0.1-0.2):1.

[0015] Preferably, the polysaccharide comprises a first polysaccharide and a second polysaccharide, wherein the first polysaccharide is a medicinal porphyra extract, and the second polysaccharide is a mixture of yeast glucan and Schizophyllan, and the weight ratio of the yeast glucan to Schizophyllan is (1-2):1.

[0016] The inventors found in the actual experimental process that the weight ratio of the active substance to the polysaccharide, and the weight ratio of the first polysaccharide to the second polysaccharide in the polysaccharide will further affect the radiation protection, whitening, oil control, and repair effects. When the active substance is a mixture of quercetin and oxidized resveratrol, the weight ratio of quercetin and oxidized resveratrol will further affect the radiation protection, whitening, and oil control effects of the radiation protection composition.

[0017] Preferably, the present invention provides a radiation protection composition, comprising the following components: an active substance and a polysaccharide, the weight ratio of the active substance to the polysaccharide = (0.5-5): (4-10); the active substance is a mixture of quercetin and oxidized resveratrol, the weight ratio of quercetin to oxidized resveratrol = (0.05-0.2): (0.5-5); the polysaccharide comprises a first polysaccharide and a second polysaccharide, the first polysaccharide is a medicinal pore fungus extract, and the second polysaccharide is a mixture of yeast glucan and schizophyllan, the weight ratio of yeast glucan to schizophyllan = (1-2): 1; the weight ratio of the first polysaccharide to the second polysaccharide = (1-10): (0.01-2).

[0018] The inventors found in the actual experimental process that the selection of active ingredients and polysaccharides in specific components further affects the effects of the radiation protection composition in radiation protection, whitening, oil control, and repair. For the selection of polyphenol active ingredients, the more phenolic hydroxyl groups there are, the higher the activity, but at the same time, the more unstable it is in aqueous solution. After a large number of experimental investigations, the inventors found that when the active ingredient is a mixture of quercetin and oxidized resveratrol, the activity is significantly increased and it can maintain good stability in aqueous solution. Quercetin and oxidized resveratrol work together to significantly enhance the radiation protection, whitening, and oil control effects of the prepared radiation protection composition.

[0019] As for the selection of polysaccharides, when the polysaccharides include a first polysaccharide and a second polysaccharide, the first polysaccharide is a medicinal pore fungus extract, and the second polysaccharide is a mixture of yeast glucan and schizophyllan, the medicinal pore fungus extract itself has two different ranges of molecular weights, which can absorb both low-dose γ radiation and high-dose γ radiation energy. When combined with yeast glucan (molecular weight 65kDa) and schizophyllan (molecular weight 436kDa), it can cover a wider range of molecular weights, not only achieving a more comprehensive radiation protection effect, but also the prepared radiation protection composition has significantly enhanced radiation protection and repair effects, and effectively reduces skin irritation.

[0020] Preferably, the method for preparing the radiation protection composition comprises the following steps: dissolving the active substance in an alcohol solvent, dissolving the polysaccharide in deionized water, and then mixing the two uniformly to obtain the radiation protection composition.

[0021] Preferably, the alcohol solvent includes 1,3-propylene glycol.

[0022] In addition, the present invention provides the use of the radiation protection composition in the preparation of skin products. Preferably, the skin product is one of lotion, emulsion, eye cream, face cream, mask, essence, sunscreen, and neck cream, and the dosage form of the skin product is one of water, emulsion, gel, spray, cream, and essence.

[0023] Furthermore, the present invention provides a skin product comprising the radiation protection composition.

[0024] The invention provides an emulsion, comprising the following components in percentage by weight: 0.05-0.3 parts of a thickener, 0.5-1 parts of a moisturizer, 0.01-0.3 parts of a pH regulator, 0.5-5 parts of an emulsifier, 0.5-3 parts of a preservative, 1-20 parts of the radiation protection composition, and the balance of deionized water.

[0025] Preferably, the emulsion comprises at least one of the following (a)-(e):

[0026] (a) the thickener comprises at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol;

[0027] (b) the moisturizing agent comprises at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butylene glycol, glycerol, budding septin polysaccharide, and ceramide;

[0028] (c) the pH adjusting agent comprises at least one of arginine, tromethamine, and EDTA-disodium;

[0029] (d) the emulsifier comprises at least one of caprylic capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetrastearate, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate;

[0030] (e) The preservative includes at least one of 1,2-hexanediol, p-hydroxyacetophenone, and acrylates / C10-30 alkyl acrylate cross-polymer.

[0031] Compared with the prior art, the present invention has the following beneficial effects: when the present invention uses a combination of specifically selected active substances and polysaccharides, it can achieve better radiation protection, oil control, whitening and repairing effects. The specific active substances have the function of blocking and absorbing gamma radiation, and the specific polysaccharides degrade into oligosaccharides or monosaccharides by absorbing the energy of gamma radiation, thereby exposing more active sites, increasing the radiation protection effect and other biological activities. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 This is a flow chart for preparing the radiation protection composition of Example 13.

[0033] Figure 2 The electrophoresis diagram is the plasmid DNA added with the radiation protection composition of Example 1-21 and Comparative Example 1-2 after being irradiated with a dose of 1 kGy.

[0034] Figure 3 The electrophoresis diagram is the plasmid DNA added with the radiation protection composition of Examples 1, 11, 13 and Comparative Example 1 after being irradiated with a dose of 3 kGy.

[0035] Figure 4 This is an emulsion diagram to which 10% of the radiation protection composition of Examples 1-21 and Comparative Examples 1-2 is added.

[0036] Figure 5 The graphs show the color changes of the emulsions to which 10% of the radiation protection compositions of Examples 1-21 and Comparative Examples 1-2 were added under different doses of gamma radiation. DETAILED DESCRIPTION

[0037] To better illustrate the purpose, technical scheme and advantages of the present invention, the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments, the purpose of which is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by ordinary technicians in the field without making creative work are within the scope of protection of the present invention. The experimental reagents and instruments designed for the implementation and comparative examples of the present invention, unless otherwise specified, are all commonly used ordinary reagents and instruments, and can be obtained from commercial channels. In the implementation and comparative examples, the experimental methods used are conventional methods unless otherwise specified.

[0038] The raw materials used in the present invention, such as quercetin, oxidized resveratrol, medicinal porphyra extract, yeast glucan, schizophyllan, myricetin, resveratrol, kaempferol, etc., are all conventional commercially available products.

[0039] The molecular weight detection method of medicinal porphyra extract, yeast glucan and schizophyllan is as follows:

[0040] The molecular weight was determined by gel filtration. Using polysaccharide standards of different molecular weights, a standard curve was drawn with the elution volume as the horizontal axis and the logarithm of the molecular weight as the vertical axis, and then the elution volume of the sample was determined for conversion.

[0041] Preparation of standard curve Weigh 5 mg of standard dextran T5, T10, T40, T70 and T500 accurately, dissolve them in 1 mL of distilled water and pass them through Sephadex G-100 chromatography columns (1.5 cm × 40 cm) respectively. Distilled water is used as the elution solvent, the flow rate is 0.2 mL / min, and 2 mL of eluate is collected in each tube. Distilled water is used as the blank control, and the anthrone-concentrated sulfuric acid method is used for tracking and monitoring. The elution curve is drawn with the tube number as the horizontal axis and the absorbance as the vertical axis. The elution volume of each standard dextran is calculated as (V e ), blue dextran was used to measure the void volume (V0) of the gel column, and glucose was used to measure the total volume V of the gel column t . Based on the effective distribution coefficient (V e / V0) as the horizontal axis and the logarithm of the molecular weight lgMr as the vertical axis to draw a standard curve.

[0042] Molecular weight determination of polysaccharide solution: Prepare 5 mg / mL polysaccharide solution to be tested, centrifuge, take the supernatant, separate according to the above elution conditions, and determine the elution volume V e , calculate the relative molecular mass based on the standard curve.

[0043] Examples 1-21 and Comparative Examples 1-2

[0044] The present application provides an anti-radiation composition. The components and weight parts of the anti-radiation compositions in Examples 1-18 and Comparative Examples 1-2 are shown in Tables 1-3. The anti-radiation compositions of Examples 19-21 are listed below. The total weight parts of the anti-radiation compositions of the embodiments and comparative examples of the present invention are 20 parts.

[0045] The preparation method of the radiation protection composition is as follows: dissolving the active substance in 1,3-propylene glycol, dissolving the polysaccharide in deionized water, and then mixing the two uniformly to obtain the radiation protection composition.

[0046] Table 1

[0047]

[0048]

[0049] Table 2

[0050]

[0051] Table 3

[0052]

[0053] Embodiment 19

[0054] A single comparison was made with Example 1, the only difference being that oxidized resveratrol was replaced with resveratrol, and the other components and weight portions remained unchanged, and the preparation method remained unchanged.

[0055] Embodiment 20

[0056] A single comparison was made with Example 7, the only difference being that quercetin was replaced with myricetin, and the other components and weight portions remained unchanged, and the preparation method remained unchanged.

[0057] Embodiment 21

[0058] A single comparison was made with Example 7, the only difference being that quercetin was replaced with kaempferol, and the other components and weight portions remained unchanged, and the preparation method remained unchanged.

[0059] Application Examples

[0060] An emulsion comprises the following components in percentage by weight: 0.05-0.3 parts of a thickener, 0.5-1 parts of a moisturizer, 0.01-0.3 parts of a pH regulator, 0.5-5 parts of an emulsifier, 0.5-3 parts of a preservative, 1-20 parts of a radiation protection composition, and the balance of deionized water.

[0061] Specifically, the present invention provides a radiation protection emulsion formula, as shown in Table 4.

[0062] Table 4 Radiation protection emulsion formula

[0063]

[0064]

[0065] The present invention provides a method for preparing a radiation-proof emulsion, which comprises the following steps:

[0066] (1) Mixing a humectant, a thickener and part of a pH adjuster with water, stirring, heating to 85° C., homogenizing at a speed of 1300 rpm for 4 minutes, and keeping the mixture warm for later use to obtain a prefabricated component A;

[0067] (2) mixing the emulsifier, heating to 75° C., homogenizing at 1300 rpm for 4 min, and keeping warm for later use after homogenization to obtain prefabricated component B;

[0068] (3) mixing the preservatives and heating to 60° C. to melt to obtain a prefabricated C component;

[0069] (4) The preformed component A is heated to 80°C, and the preformed component B is added at a speed of 300 rpm, and stirred and mixed. Then, the temperature is lowered to 60°C, and the preformed component C is added at a speed of 300 rpm, and stirred and mixed. Then, the temperature is lowered to below 45°C, and the radiation protection composition is added and stirred for 8 minutes. Finally, the remaining pH adjuster is added to adjust the pH to 5.5-7.0, and the stirring can be stopped. The material is discharged to obtain the cosmetic.

[0070] Performance Testing

[0071] 1. DNA protection experiment under gamma radiation.

[0072] Experimental method: The plasmid DNA carrying the human gene (pcDNA3.1-STAT5A, Guangzhou Huijun Biotechnology Co., Ltd.) was dissolved in the radiation protection composition provided in Example 1-21 and Comparative Example 1-2, and the final concentration of DNA was 50 ng / μL. 60 Gamma ray irradiation was performed using a Co source (Guangzhou HuaDa Biotechnology Co., Ltd.) at a dose of 1 kGy and 3 kGy. During the irradiation process, the samples were placed on ice. DNA gel electrophoresis was performed on the irradiated DNA samples to obtain electrophoretic patterns corresponding to three components: supercoiled (SC), linear (L), and open-circular (OC) DNA.

[0073] Complete plasmid DNA should be in supercoil form. The higher the proportion of SC, the more complete the plasmid DNA is. When one of the chains of the plasmid DNA is broken, an open loop state OC is formed. When both chains of the plasmid DNA are broken, it is in linear form L. If the DNA is completely degraded into small molecular fragments, the electrophoresis graph will show a tailing shape. DNA integrity is calculated using the following formula:

[0074] DNA integrity = (f SC +1 / 2*f OC ) / (f SC +f OC +f L ).

[0075] f in the formula SC 、f OC 、f L They represent the proportion of the corresponding fragments respectively.

[0076] Experimental results: as shown in Table 5. Figure 2 , Figure 3 shown.

[0077] Table 5 DNA integrity at irradiation doses of 1 kGy and 3 kGy

[0078]

[0079]

[0080] As can be seen from the table above, the results of the DNA protection experiment under irradiation doses of 1kGy and 3kGy show that when no measures are taken, the plasmid DNA under 1kGy radiation has no supercoiled DNA at all, only open-circular and linear DNA, and the DNA integrity is only 10%, while the plasmid DNA under 3kGy radiation has been completely degraded, and both chains are 100% broken. After protection with the radiation protection composition prepared by the present invention, when the irradiation dose is 1kGy, the DNA integrity is more than 45%, and when the irradiation dose is 3kGy, the DNA integrity is more than 20%, and the integrity of the plasmid DNA is significantly improved. The present invention can achieve a good radiation protection effect when the specifically selected active substance and polysaccharide are used in combination.

[0081] 2. Stability experiment under gamma radiation.

[0082] According to the radiation protection emulsion formula of Table 4, an emulsion containing 10% w / w radiation protection composition was prepared and placed in a 10 mL transparent plastic bottle. Figure 4 The emulsion containing the radiation protection composition is 60 γ-ray irradiation produced by Co source (Guangzhou Huada Biotechnology Co., Ltd.), the irradiation dose was 3kGy, 6kGy, and 9kGy. The color change of the emulsion was observed before and after irradiation.

[0083] The test results are as follows Figure 5 As shown, after testing, the color of the emulsion containing the radiation protection composition provided by the present invention remains stable under γ radiation with a dose of 0-9 kGy, indicating that the formula system can remain stable under γ radiation.

[0084] 3. Human skin patch test.

[0085] 30 volunteers were recruited, 15 males and 15 females, aged 20-50 years old, and the closed patch test method was used. An equal amount (0.020mL-0.025mL) of the test sample (emulsion containing 10% w / w composition) was placed in a specific patch tester, which was applied to the volunteer's arm with a low-allergenic tape, and gently pressed to evenly apply it on the skin for 24 hours; the blank control group was distilled water, and the blank application example was an emulsion without the radiation protection composition. After 24 hours, the patch tester was removed, and the skin reaction was observed after 0.5 hours, 24 hours, and 48 hours, and the results were recorded. The level of adverse reactions to the skin is shown in Table 6 below.

[0086] The test results are shown in Table 7 below.

[0087] Table 6

[0088]

[0089] Table 7

[0090]

[0091]

[0092]

[0093]

[0094] The test results show that there were no cases of withdrawal and no cases of allergic reactions to the adhesive tape in the technical solution provided by the present invention. Among them, no adverse reactions occurred in Examples 1-21 and Comparative Example 1; and 5 cases of Grade 1 reactions occurred in Comparative Example 2 within 0.5h, indicating that when the amount of polysaccharide added was too low, the obtained emulsion containing the radiation protection composition had a certain irritation.

[0095] 4. Experiment on the efficacy of instant oil control on human body.

[0096] In the composition, the medicinal pore fungus extract has a good effect of inhibiting the activity of 5α-reductase, thereby inhibiting the sebaceous glands from secreting oil, solving the problem of excessive skin oil secretion from the source; quercetin is a flavonoid compound of natural origin, which has the effect of inhibiting lipid peroxidation, thereby unclogging hair follicles, tightening pores, and effectively reducing oil secretion. This experiment found that the combination of medicinal pore fungus extract and quercetin has a stronger oil control effect, and oxidized resveratrol, yeast glucan and schizophyllan can work together with medicinal pore fungus extract and quercetin within the specific dosage range of the present invention to achieve good oil control effect.

[0097] Experimental method: According to the "Technical Specifications for Safety of Cosmetics" (2015), 69 Asian adult testers aged 18-60 with oily skin were selected and randomly divided into 23 groups, 3 people in each group. On the day of the volunteer's visit, the volunteers sat in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10% for 10 minutes and then washed their faces with a soap-based facial cleanser. A skin oil test probe (Sebumeter SM815) was used to collect the forehead oil content data at 0h to ensure that the volunteers' facial oil content was within 5μg / cm 2 The volunteers applied the sample (the sample was an emulsion containing 10% w / w of the radiation protection composition according to the radiation protection emulsion formula in Table 4) on half of their faces, and a placebo on the other half of their faces, and the forehead oil content data were collected at 2h and 4h.

[0098] Skin oil secretion A = T Xh Oil content-T 0h Oil content.

[0099] Skin oil secretion inhibition rate = (A placebo - A sample) / A placebo * 100%.

[0100] Experimental results: as shown in Table 8.

[0101] Table 8

[0102]

[0103]

[0104] Note: * indicates that the difference is statistically significant compared with placebo (P<0.05).

[0105] As can be seen from the above table, the oil secretion inhibition rate of Comparative Examples 1-2 is extremely poor compared with Examples 1-21, while the oil secretion inhibition rate of Example 4 is relatively poor compared with all other examples, indicating that the amount of the medicinal pore fungus extract in the radiation protection composition provided by the present invention has a great influence on the oil control effect.

[0106] From the comparison of Example 1, Examples 7-9, and Examples 13-15, it can be seen that for the radiation protection composition provided by the present invention, the synergistic use of the medicinal porphyra extract and quercetin, combined with the specific oxidized resveratrol, yeast glucan and schizophyllan of the present invention, can greatly increase the oil control effect of the radiation protection composition, and when the weight ratio of quercetin to oxidized resveratrol is (0.1-0.2):1, the oil control effect is significantly improved.

[0107] From the comparison of Example 1, Example 7, and Examples 20-21, it can be seen that when the radiation protection composition contains quercetin, the synergy of the components can be better exerted to enhance the oil control effect.

[0108] 5. Experiment on the efficacy of whitening and lightening effect on human body.

[0109] Quercetin, oxidized resveratrol and medicinal pore fungus extract synergistically whiten and lighten spots. Quercetin inhibits post-inflammatory pigmentation, oxidized resveratrol inhibits tyrosinase activity, and medicinal pore fungus extract reduces oxidative dullness caused by excessive facial oil and brightens skin tone. Within the specific dosage range of the present invention, the second polysaccharide can work synergistically with quercetin, oxidized resveratrol and medicinal pore fungus extract to achieve good whitening and lightening effects.

[0110] Experimental method: According to the "Technical Specifications for Safety of Cosmetics" (2015), 72 Asian adult testers aged 18-60 years old, with facial skin chroma of 28 or above and facial pigmentation area >3mm were selected and randomly divided into 24 groups, 3 people in each group. The volunteers used the samples on their entire faces for 28 days to evaluate the whitening and spot-lightening effects of the samples in different groups (the samples were prepared according to the radiation-proof emulsion formula in Table 4, containing 10% w / w radiation-proof composition). The specific steps are as follows:

[0111] Every morning and evening, after cleansing and moisturizing, volunteers should apply an appropriate amount of lotion on their face. This should be used on the entire face once in the morning and once in the evening, with follow-up visits on the 14th and 28th days. On the day of the volunteer's visit, the test area should wash their face with clean water without applying any products, and sit quietly for 20 minutes in an air-conditioned room with a temperature of 21±1°C and a humidity of 50±10%. The CK skin color test probe CL400 was used to measure the ITA value of the non-spot area, and the MX18 was used to measure the melanin content MI value of the spot area. The change in the ITA value can represent the improvement in skin color. The smaller the value, the whiter the skin color. The change in the MI value can represent the degree of improvement in spots. The smaller the value, the more obvious the fading of the spots. In this experiment, the improvement in spots is represented by the improvement rate of each value, and the calculation method is as follows:

[0112] Mean improvement rate relative to blank value = (X before use - X after use) / X before use × 100%

[0113] Experimental results: as shown in Table 9.

[0114] Table 9

[0115]

[0116]

[0117] Note: * indicates that the difference is statistically significant compared with the blank application example (P<0.05).

[0118] As can be seen from the above table, compared with Example 1, Example 13 and Example 17, the improvement rate of the ITA value in the non-spot area and the MI value in the spot area of ​​Comparative Examples 1-2 is significantly reduced, and the medicinal porphyra extract has a great influence on the whitening and spot-removing effect in the radiation protection composition provided by the present invention.

[0119] From the comparison of Example 1, Examples 7-9, and Examples 13-15, it can be seen that for the radiation protection composition provided by the present invention, the synergistic use of oxidized resveratrol and the medicinal porphyra extract, combined with the specific quercetin, yeast glucan and schizophyllan of the present invention, can greatly increase the whitening and spot-removing effect of the radiation protection composition, and when the weight ratio of quercetin to oxidized resveratrol is (0.1-0.2):1, the whitening and spot-removing effect is significantly improved.

[0120] 6. Experiment on the effect of repairing human body.

[0121] Polysaccharides form a protective film on the surface of the skin, which can reduce water loss and the invasion of external irritants, and help repair and strengthen the skin barrier function. Yeast glucan can activate the immune cells of the skin, enhance immune function, promote collagen production, and thus promote skin damage repair. Schizophyllum polysaccharide has excellent moisturizing ability. It can absorb and lock moisture, increase the water content of the skin, and improve problems such as dry and rough skin. The medicinal porphyra extract not only has a moisturizing effect, but also has an anti-inflammatory effect, which can reduce skin inflammatory reactions, relieve redness and tingling, and promote skin repair and recovery. Within the specific dosage range of the present invention, the active ingredients can synergistically repair the skin barrier with the first polysaccharide and the second polysaccharide, and play a good stratum corneum barrier repair effect.

[0122] Experimental method: 40 Asian adult test subjects were selected and randomly divided into 8 groups, with 5 people in each group. The volunteers were subjected to the arm tape tearing experiment. The specific steps are as follows:

[0123] On the day of the visit, the volunteers sat quietly in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10% for 20 minutes. Two 2.5cm×4cm (10cm) areas were selected on each forearm to establish four test areas. Before the test, a skin moisture loss test probe ( TM Hex) was used to measure the initial transepidermal water loss value TEWL (T0) of the forearm, and then the tape was stripped 30 times, and TEWL (T0') was measured 10 minutes later. The numerical value between T0' and T0 was calculated to determine whether the modeling was established. Then, the test sample (the sample was an emulsion containing 10% w / w of the radiation protection composition prepared according to the radiation protection emulsion formula in Table 4) and the placebo were randomly applied to a test area, and the TEWL value (T2, T4) of the test area was detected at 2h and 4h. Three samples and one placebo control were tested for each group of volunteers.

[0124] Skin transepidermal water loss change rate V = (T0'-TX) / (T0'-T0)*100%.

[0125] Improvement rate of transepidermal water loss = V sample - V placebo.

[0126] The transepidermal water loss (TEWL) value is a commonly used indicator to reflect the barrier function of the stratum corneum. The lower the TEWL value, the better the skin barrier function. After the tape was peeled off on the arm, the stratum corneum of the skin was damaged and the TEWL value increased. Then the skin recovered spontaneously and the TEWL value decreased over time. Samples with repair ability can accelerate this process.

[0127] Experimental results: as shown in Table 10.

[0128] Table 10

[0129]

[0130]

[0131] Note: * indicates that the difference is statistically significant compared with placebo (P<0.05).

[0132] It can be seen from the above table that, by comparing Comparative Example 2 and Examples 1-4, the total amount of the first polysaccharide and the second polysaccharide in the radiation protection composition provided by the present invention has a great influence on the repair effect.

[0133] From the comparison of Example 1, Examples 5-6, and Examples 16-18, it can be seen that when the weight ratio of the first polysaccharide to the second polysaccharide = (10-40):1, the improvement rate of transepidermal water loss is higher and the effect of repairing the skin barrier is better.

[0134] From the comparison of Example 1 and Examples 10-15, it can be seen that for the radiation protection composition provided by the present invention, the synergistic use of the first polysaccharide and the second polysaccharide, combined with the specific active ingredient of the present invention, can greatly improve the skin barrier repair effect, and when the weight ratio of yeast glucan to schizophyllan is (1-2):1, the skin transepidermal water loss improvement rate is higher, and the effect of repairing the skin barrier is better.

[0135] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A radiation protection composition, characterized in that: The invention is composed of the following components: an active substance and a polysaccharide, wherein the weight ratio of the active substance to the polysaccharide is (0.1-5): (3-10); The active substance is a mixture of quercetin and oxidized resveratrol, or oxidized resveratrol; The polysaccharide comprises a first polysaccharide and a second polysaccharide, wherein the first polysaccharide is an extract of medicinal porphyra, and the second polysaccharide is a mixture of schizophyllan and yeast glucan, or yeast glucan; The weight ratio of the first polysaccharide to the second polysaccharide is (1-10): (0.01-2).

2. The radiation protection composition according to claim 1, characterized in that The weight ratio of the active ingredient to the polysaccharide is (2-3): (7-8).

3. The radiation protection composition according to claim 1, characterized in that The weight ratio of the first polysaccharide to the second polysaccharide is (10-40):

1.

4. The radiation protection composition according to claim 1, characterized in that The active substance is a mixture of quercetin and oxidized resveratrol, and the weight ratio of the quercetin to the oxidized resveratrol is (0.05-0.2): (0.5-5).

5. The radiation protection composition according to claim 4, characterized in that The weight ratio of quercetin to oxidized resveratrol = (0.1-0.2):

1.

6. The radiation protection composition according to claim 3, characterized in that: The polysaccharide comprises a first polysaccharide and a second polysaccharide, wherein the first polysaccharide is an extract of medicinal porphyra, and the second polysaccharide is a mixture of yeast glucan and schizophyllan, and the weight ratio of the yeast glucan to schizophyllan is (1-2):

1.

7. Use of the radiation protection composition according to any one of claims 1 to 6 in the preparation of skin products.

8. A skin care product, characterized in that: Made from the radiation protection composition according to any one of claims 1 to 6; the skin product is one of lotion, emulsion, eye cream, face cream, facial mask, essence, sunscreen and neck cream.

9. An emulsion, characterized in that The invention comprises the following components in percentage by weight: 0.05-0.3 parts of thickener, 0.5-1 parts of moisturizer, 0.01-0.3 parts of pH regulator, 0.5-5 parts of emulsifier, 0.5-3 parts of preservative, 1-20 parts of the radiation protection composition according to any one of claims 1-6, and the balance of deionized water.

10. The emulsion according to claim 9, characterized in that Contains at least one of the following (a)-(e): (a) the thickener comprises at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol; (b) the moisturizing agent comprises at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butylene glycol, glycerol, budding septin polysaccharide, and ceramide; (c) the pH adjusting agent comprises at least one of arginine, tromethamine, and EDTA-disodium; (d) the emulsifier comprises at least one of caprylic capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetrastearate, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate; (e) The preservative includes at least one of 1,2-hexanediol, p-hydroxyacetophenone, and acrylates / C10-30 alkyl acrylate crosspolymer.

Images