Intelligent control fermentation device and fermentation method

Abstract

The present invention relates to the technical field of fermentation equipment, and discloses an intelligent control fermentation device and a fermentation method. The present invention detects the liquid level height inside the fermentation tank body through a liquid level sensor, detects the liquid temperature inside the fermentation tank body through a temperature sensor, and detects the liquid sugar content inside the fermentation tank body through a sugar content detection mechanism. The control system can calculate the detection data according to the detection data returned by the detection system, obtain the current fermentation process inside the fermentation tank body, and accurately divide the fermentation inside the fermentation tank body. Then, according to the changes in the fermentation process, the control system controls the opening and closing state of the feed port, the opening and closing state of the discharge port, the stirring power of the stirring mechanism, and the temperature inside the fermentation tank body, so as to accurately control the progress of each process inside the fermentation tank.

Description

Technical Field

[0001] The present invention relates to the technical field of fermentation equipment, and in particular to an intelligent control fermentation device and a fermentation method. Background Art

[0002] Fermentation refers to the process in which people use the life activities of microorganisms under aerobic or anaerobic conditions to prepare microbial cells themselves, or direct metabolites or secondary metabolites. Fermentation usually refers to a certain decomposition process of organic matter by organisms. Fermentation is a biochemical reaction that humans have been exposed to for a long time, and it is now widely used in the food industry, biological and chemical industries. The modern definition of fermentation is: through large-scale growth and cultivation of microorganisms (or animal and plant cells), chemical and physiological changes occur in them, thereby producing and accumulating a large number of metabolites needed for fermentation.

[0003] Fermentation is a chemical reaction and physiological change carried out by living organisms. It is a process in which various biochemical reactions continuously decompose and synthesize according to the genetic information of the living organisms themselves to obtain energy to maintain life activities. Fermentation products refer to substances that can regulate metabolism to achieve balance during the reaction process or when the reaction reaches the end point. In fact, fermentation is also a kind of respiration, but the usual respiration refers to aerobic respiration, and the final result is the production of carbon dioxide and water, while the fermentation process is an anaerobic respiration process, and the final result is the production of alcohol, carbon dioxide and other metabolic products.

[0004] In the fermentation industry, the fermentation process is divided into batch fermentation, continuous fermentation and fed-batch fermentation. Batch fermentation: the feed liquid is put into the fermentation tank at one time. Except for the introduction of sterile air and the discharge of waste gas, there is no material exchange with the outside world during the fermentation process until the fermentation is completed and the tank is released. Continuous fermentation: It is carried out in a specific fermentation equipment. Fresh sterile feed liquid is continuously input while the fermentation liquid is continuously discharged. It can be divided into tank continuous fermentation and pipeline continuous fermentation. Fed-batch fermentation: A fermentation operation method between batch fermentation and continuous fermentation. It refers to the culture method in which fresh feed liquid of one or more components is intermittently or continuously added to the fermentation tank during the culture process. It is also called semi-continuous culture.

[0005] In actual fed-batch fermentation, the judgment of each fermentation process is usually based on experience or temporary sampling and testing, which leads to inaccurate judgment process and delayed judgment results, which in turn leads to prolonged fermentation process and imprecise control of the fermentation process, which can easily lead to the problem of over-fermentation.

[0006] Currently, no effective solution has been proposed for the problems in the related technologies. Summary of the invention

[0007] In view of the deficiencies in the prior art, the present invention provides an intelligent controlled fermentation device and a fermentation method.

[0008] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0009] An intelligent controlled fermentation device comprises a fermentation tank body, a sealed tank cover and a stirring mechanism, wherein the fermentation tank body and the sealed tank cover are sealed, a feed port is arranged at the top of the sealed tank cover, a liquid discharge port is arranged at the bottom of the fermentation tank body, the stirring mechanism can stir the inside of the fermentation tank body, a heat preservation jacket is arranged outside the fermentation tank body, the heat preservation jacket can exchange temperature and keep the inside of the fermentation tank body warm, and the temperature inside the fermentation tank body can be adjusted by adjusting the power of the heat preservation jacket; a detection system is arranged inside the fermentation tank body, the detection system comprises a liquid level sensor, a temperature sensor and a sugar content detection mechanism, the liquid level sensor is used to detect the liquid level height inside the fermentation tank body, and the temperature sensor is used to detect the sugar content inside the fermentation tank body The sugar content detection mechanism is used to detect the sugar content of the liquid inside the fermentation tank body; a control system is also provided on the outside of the fermentation tank body, and the control system can receive the detection data sent back by the detection system, and the detection data includes the liquid level height data, temperature and liquid sugar content inside the fermentation tank body, and the control system can calculate the detection data to obtain the current fermentation process inside the fermentation tank body; solenoid valves are provided on the outsides of the feed port and the drain port, and the opening and closing of the corresponding feed port and the drain port can be controlled by controlling the opening and closing of the solenoid valves; the control system can control the opening and closing state of the feed port, the opening and closing state of the drain port, the stirring power of the stirring mechanism and the temperature inside the fermentation tank body according to the changes in the fermentation process.

[0010] Preferably, the sugar content detection mechanism includes an auxiliary detection cavity, which is arranged on one side of the bottom end of the fermentation tank body, and is communicated with the interior of the fermentation tank body; a solid filter plate is arranged between the auxiliary detection cavity and the fermentation tank body, and the liquid inside the fermentation tank body can pass through the solid filter plate and enter the auxiliary detection cavity; a refractive index detection mechanism is arranged inside the auxiliary detection cavity, and the refractive index detection mechanism can detect the refractive index of the liquid inside the auxiliary detection cavity, and the sugar content of the liquid inside the auxiliary detection cavity can be obtained according to the refractive index of the liquid.

[0011] Preferably, the refractive index detection mechanism includes a laser transmitter, a laser receiver and an ultrasonic defoamer; there is liquid between the laser transmitter and the laser receiver that enters the auxiliary detection chamber from the interior of the fermentation tank body, and the ultrasonic defoamer can emit ultrasound to break the bubbles in the liquid between the laser transmitter and the laser receiver; the laser transmitter can emit laser to the laser receiver, and the laser receiver can receive laser, obtain the refractive index of the liquid between the laser transmitter and the laser receiver, and convert the sugar content of the liquid according to the refractive index of the liquid.

[0012] Preferably, the fermentation process includes a sterilization stage, an initial feeding stage, n cyclic fermentation stages and a final discharge stage; each of the cyclic fermentation stages includes a fermentation stage, a discharge stage and a feeding stage;

[0013] The real-time sugar content of the liquid obtained by the detection system is P i , the real-time height of the liquid level is H i , the real-time temperature of the liquid is T i ; In the initial feeding stage, the sugar content of the mixed liquid of the fermentation liquid and the bacterial liquid entering is P0, and the total amount is H0. In the subsequent feeding stage, the sugar content of the fermentation liquid added is kP0;

[0014] At any fermentation stage, P i From P0 to gP0;

[0015] At any discharge stage, P i Remain unchanged, H i Reduce to fH0;

[0016] In any feeding stage, fermentation liquid with sugar content kP0 is fed. i Return to H0, P i Return to P0;

[0017] In the final discharge stage, H i Reduce to 0 and completely empty the fermenter.

[0018] Preferably, in any of the cyclic fermentation stages in a fermentation process, n, P0, H0, g, f, and k are all constants, and n≤10, k≥1.2, 0.3≥g≥0.15, 0.3≥f≥0.15; g, f, and k satisfy the relationship: k(1-f)+gf=1.

[0019] Preferably, during the sterilization stage, high-temperature steam is introduced into the sealed fermentation tank body and the sealed tank cover to maintain the internal temperature of the fermentation tank body not lower than 120° C. After 20-30 minutes of treatment, the introduction of high-temperature steam is stopped, and the internal temperature of the fermentation tank body is allowed to drop to room temperature;

[0020] In the initial feeding stage, sterile fermentation liquid with a sugar content of kP0 is introduced into the fermentation tank, and then the bacterial liquid is introduced, and the stirring mechanism is started to mix the fermentation liquid and the bacterial liquid at 75 rad / min until the total amount of the fermentation liquid and the bacterial liquid mixture is H0 and the sugar content is P0;

[0021] Any fermentation stage includes a bacterial growth stage and a bacterial fermentation stage. In the bacterial growth stage, the temperature inside the fermentation tank is maintained at 16-18°C by the heat preservation jacket. In the bacterial fermentation stage, the temperature inside the fermentation tank is maintained at 19-22°C by the heat preservation jacket. In the bacterial growth stage and the bacterial fermentation stage, the stirring speed of the stirring mechanism is 30rad / min.

[0022] At any discharging stage, the stirring speed of the stirring mechanism is 75 rad / min;

[0023] In any feeding stage, the stirring speed of the stirring mechanism is 75 rad / min;

[0024] In the last discharging stage, the stirring speed of the stirring mechanism is 75 rad / min.

[0025] Preferably, a ventilation interface is also provided on the top of the sealed tank cover, and a dissolved oxygen monitor is also provided inside the fermentation tank body. The ventilation interface is externally connected to a ventilation filter and a ventilation pump. The ventilation interface is also externally connected to a pressure relief valve, and the pressure relief valve is used to release the internal pressure of the fermentation tank body. The dissolved oxygen monitor can detect the dissolved oxygen content inside the fermentation tank body, and the ventilation pump can exchange gas between external air and the internal gas of the fermentation tank body through the ventilation filter according to the change of the dissolved oxygen content inside the fermentation tank body.

[0026] Preferably, the stirring mechanism includes a stirring motor, a stirring connecting rod and a stirring piece, the stirring motor is fixed at the top end of the sealed tank cover, and the stirring motor drives the stirring piece to rotate through the stirring connecting rod; the stirring piece includes a stirring outer frame and a plurality of stirring fan blades, the stirring outer frame is close to the inner wall of the fermentation tank body, when the stirring piece is driven to rotate, the stirring outer frame can stir the liquid inside the fermentation tank body to flip in the horizontal direction, and the stirring fan blades can stir the liquid inside the fermentation tank body to flip in the vertical direction.

[0027] Preferably, a discharge port is further provided at the bottom end of the fermentation tank body, and the front end of the stirring outer frame along the forward direction is inclined toward the rear end in the forward direction. When the stirring outer frame rotates, it can scrape off the attachments on the inner wall of the fermentation tank body and discharge them from the discharge port.

[0028] A fermentation method uses the above-mentioned intelligent control fermentation device.

[0029] Compared with the prior art, the present invention provides an intelligent control fermentation device and a fermentation method, which have the following beneficial effects:

[0030] 1. This intelligent control fermentation device performs a batch fermentation process inside a sealed fermentation tank body. When in use, the fermentation tank body can be stirred by a stirring mechanism, and the temperature inside the fermentation tank body can be adjusted by adjusting the power of the insulation jacket. During the use of this intelligent control fermentation device, the liquid level inside the fermentation tank body can be detected by a liquid level sensor, the liquid temperature inside the fermentation tank body can be detected by a temperature sensor, and the sugar content detection mechanism can detect the sugar content of the liquid inside the fermentation tank body. Then, the above-mentioned real-time detection data is sent to the control system. The control system can calculate the detection data based on the detection data returned by the detection system, including the liquid level data, temperature and liquid sugar content inside the fermentation tank body. The control system can calculate the detection data to obtain the current fermentation process inside the fermentation tank body, so as to accurately divide the fermentation inside the fermentation tank body. Then, according to the changes in the fermentation process, the control system controls the opening and closing state of the feed port, the opening and closing state of the discharge port, the stirring power of the stirring mechanism and the temperature inside the fermentation tank body, so as to accurately control the progress of each process inside the fermentation tank, thereby improving the fermentation effect, improving the fermentation efficiency, saving the fermentation time of the entire batch, and improving the benefits of fermentation production.

[0031] 2. This intelligent controlled fermentation device, through the setting of a solid filter plate, allows the liquid inside the fermentation tank body to pass through the solid filter plate and enter the auxiliary detection chamber, blocking the solids such as mycelium and large-particle fermentation raw materials inside the fermentation tank body outside the solid filter plate, which can prevent the solids such as mycelium and large-particle fermentation raw materials from interfering with the refractive index detection of the refractive index detection mechanism inside the auxiliary detection chamber. At the same time, the ultrasonic defoamer emits ultrasound to break the bubbles in the liquid between the laser transmitter and the laser receiver, preventing the bubbles generated by the fermentation from interfering with the refractive index, thereby effectively ensuring the detection accuracy of the refractive index detection mechanism, and then ensuring the accuracy and effectiveness of the measured sugar content, and can provide accurate data support for the judgment of the fermentation process of the control system, thereby effectively controlling the changes in the fermentation process and improving the fermentation efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 This is one of the three-dimensional structural schematic diagrams of an intelligent controlled fermentation device of the present invention;

[0033] Figure 2 This is the second schematic diagram of the three-dimensional structure of an intelligent control fermentation device of the present invention;

[0034] Figure 3 This is a schematic diagram of the internal structure of an intelligent control fermentation device of the present invention;

[0035] Figure 4 It is a three-dimensional structural schematic diagram of the sugar content detection mechanism of the present invention;

[0036] Figure 5 It is a schematic diagram of the internal structure of the sugar content detection mechanism of the present invention;

[0037] Figure 6 It is a cross-sectional schematic diagram of the sugar content detection mechanism of the present invention;

[0038] Figure 7 It is a schematic diagram of the three-dimensional structure of the stirring element of the present invention.

[0039] In the figure: 1. fermentation tank body; 11. drain port; 12. discharge port; 2. sealed tank cover; 21. feed port; 22. ventilation interface; 3. stirring mechanism; 31. stirring motor; 32. stirring connecting rod; 33. stirring element; 331. stirring frame; 332. stirring fan blade; 4. insulation jacket; 5. detection system; 51. liquid level sensor; 52. temperature sensor; 53. sugar content detection mechanism; 531. auxiliary detection chamber; 532. solid filter plate; 533. refractive index detection mechanism; 5331. laser transmitter; 5332. laser receiver; 5333. ultrasonic defoamer. DETAILED DESCRIPTION

[0040] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0041] As introduced in the background technology, there are deficiencies in the prior art. In order to solve the above technical problems, the present application proposes an intelligent control fermentation device and a fermentation method.

[0042] Embodiment 1:

[0043] Please refer to Figure 1-Figure 7, an intelligent controlled fermentation device, comprising a fermentation tank body 1, a sealed tank cover 2 and a stirring mechanism 3, the fermentation tank body 1 and the sealed tank cover 2 are sealed, a feed port 21 is arranged at the top of the sealed tank cover 2, a liquid discharge port 11 is arranged at the bottom of the fermentation tank body 1, the stirring mechanism 3 can stir the inside of the fermentation tank body 1, a heat preservation jacket 4 is arranged outside the fermentation tank body 1, the heat preservation jacket 4 can exchange and keep the temperature inside the fermentation tank body 1, and the temperature inside the fermentation tank body 1 can be adjusted by adjusting the power of the heat preservation jacket 4; a detection system 5 is arranged inside the fermentation tank body 1, the detection system 5 comprises a liquid level sensor 51, a temperature sensor 52 and a sugar content detection mechanism 53, the liquid level sensor 51 is used to detect the liquid level height inside the fermentation tank body 1, and the temperature sensor 52 is used to detect The liquid temperature inside the fermentation tank body 1 is measured, and the sugar content detection mechanism 53 is used to detect the sugar content of the liquid inside the fermentation tank body 1; a control system is also provided on the outside of the fermentation tank body 1, and the control system can receive the detection data sent back by the detection system 5, and the detection data includes the liquid level height data, temperature and liquid sugar content inside the fermentation tank body 1. The control system can calculate the detection data to obtain the current fermentation process inside the fermentation tank body 1; solenoid valves are provided on the outside of the feed port 21 and the discharge port 11, and the opening and closing of the corresponding feed port 21 and the discharge port 11 can be controlled by controlling the opening and closing of the solenoid valve; the control system can control the opening and closing state of the feed port 21, the opening and closing state of the discharge port 11, the stirring power of the stirring mechanism 3 and the temperature inside the fermentation tank body 1 according to the changes in the fermentation process.

[0044] Among them, the liquid level sensor 51 is a common liquid level sensor (static pressure level gauge / liquid level transmitter / liquid level sensor / water level sensor) in the prior art, and the type and working principle of the specific liquid level sensor 51 used are not described in detail here; the temperature sensor 52 is a common thermocouple temperature sensor in the prior art, and its specific working principle and specific structure are not described in detail here;

[0045] When in use, the fermentation tank body 1 and the sealed tank cover 2 are first sealed, and then a batch of fermentation processes are carried out inside the sealed fermentation tank body 1 (the raw material used in this intelligent control fermentation device is fermentation liquid, which can be a solid-liquid mixed fermentation liquid). When in use, the fermentation tank body 1 can be stirred by the stirring mechanism 3, and the temperature inside the fermentation tank body 1 can be adjusted by adjusting the power of the insulation jacket 4. During the use of this intelligent control fermentation device, the liquid level inside the fermentation tank body 1 can be detected by the liquid level sensor 51, the temperature sensor 52 detects the liquid temperature inside the fermentation tank body 1, and the sugar content detection mechanism 53 detects the sugar content of the liquid inside the fermentation tank body 1. Then the above-mentioned real-time detection data , sent to the control system. The control system can calculate the detection data based on the detection data sent back by the detection system 5, and the detection data include the liquid level height data, temperature and liquid sugar content inside the fermentation tank body 1. The control system can obtain the current fermentation process inside the fermentation tank body 1 to accurately divide the fermentation inside the fermentation tank body 1. Then, the control system controls the opening and closing state of the feed port 21, the opening and closing state of the discharge port 11, the stirring power of the stirring mechanism 3 and the temperature inside the fermentation tank body 1 according to the changes in the fermentation process, so as to accurately control the progress of each process flow inside the fermentation tank 1, improve the fermentation effect, improve the fermentation efficiency, save the fermentation time of the entire batch, and improve the efficiency of fermentation production.

[0046] For further information, please refer to Figure 1-Figure 6 The sugar content detection mechanism 53 includes an auxiliary detection chamber 531, which is arranged on one side of the bottom end of the fermentation tank body 1, and the auxiliary detection chamber 531 is communicated with the interior of the fermentation tank body 1. A solid filter plate 532 is arranged between the auxiliary detection chamber 531 and the fermentation tank body 1, and the liquid inside the fermentation tank body 1 can pass through the solid filter plate 532 to enter the auxiliary detection chamber 531; a refractive index detection mechanism 533 is arranged inside the auxiliary detection chamber 531, and the refractive index detection mechanism 533 can detect the refractive index of the liquid inside the auxiliary detection chamber 531, and the sugar content of the liquid inside the auxiliary detection chamber 531 can be obtained according to the refractive index of the liquid.

[0047] The refractive index detection mechanism 533 includes a laser transmitter 5331, a laser receiver 5332 and an ultrasonic defoamer 5333; there is liquid between the laser transmitter 5331 and the laser receiver 5332 that enters the auxiliary detection chamber 531 from the inside of the fermentation tank body 1, and the ultrasonic defoamer 5333 can emit ultrasound to break the bubbles in the liquid between the laser transmitter 5331 and the laser receiver 5332; the laser transmitter 5331 can emit laser to the laser receiver 5332, and the laser receiver 5332 can receive the laser, obtain the refractive index of the liquid between the laser transmitter 5331 and the laser receiver 5332, and convert the sugar content of the liquid according to the refractive index of the liquid.

[0048] When in use, the laser receiver 5332 of the refractive index detection mechanism 533 can receive laser light and obtain the refractive index of the liquid between the laser transmitter 5331 and the laser receiver 5332. The measuring principle is to determine the refractive index of the material through the relationship between the propagation speed of light in the material and the refractive index of the material, thereby obtaining the refractive index of the sample. In actual operation, the refractive index of the sample is first measured with a refractometer (generally called Brix value), and then converted into a sugar content value through a conversion formula. In this embodiment, the sugar content is the percentage of sugar content, and the percentage of sugar content = 136.4 / (refractive index value-130)-0.1;

[0049] In actual use, the solid filter plate 532 can be set so that the liquid inside the fermentation tank body 1 can pass through the solid filter plate 532 and enter the auxiliary detection chamber 531, and the solids such as mycelium and large-particle fermentation raw materials inside the fermentation tank body 1 are blocked outside the solid filter plate 532, so as to avoid the solids such as mycelium and large-particle fermentation raw materials from interfering with the refractive index detection of the refractive index detection mechanism 533 inside the auxiliary detection chamber 531. At the same time, the ultrasonic defoamer 5333 emits ultrasound to break the bubbles in the liquid between the laser transmitter 5331 and the laser receiver 5332, so as to prevent the bubbles generated by fermentation from interfering with the refractive index, thereby effectively ensuring the detection accuracy of the refractive index detection mechanism 533, and then ensuring the accuracy and effectiveness of the measured sugar content, and providing accurate data support for the judgment of the fermentation process of the control system, so as to effectively control the changes in the fermentation process and improve the efficiency of the fermentation.

[0050] Furthermore, the fermentation process includes a sterilization stage, an initial feeding stage, n cyclic fermentation stages and a final discharge stage; each cyclic fermentation stage includes a fermentation stage, a discharge stage and a feeding stage;

[0051] The real-time sugar content of the liquid obtained by the detection system 5 is P i , the real-time height of the liquid level is H i , the real-time temperature of the liquid is T i ; In the initial feeding stage, the sugar content of the mixed liquid of the fermentation liquid and the bacterial liquid entering is P0, and the total amount is H0. In the subsequent feeding stage, the sugar content of the fermentation liquid added is kP0;

[0052] At any fermentation stage, P i From P0 to gP0;

[0053] At any discharge stage, P i Remain unchanged, H i Reduce to fH0;

[0054] In any feeding stage, fermentation liquid with sugar content kP0 is fed.i Return to H0, P i Return to P0;

[0055] In the final discharge stage, H i Lower to 0 and completely empty the fermenter 1.

[0056] In any cyclic fermentation stage of a fermentation process, n, P0, H0, g, f, and k are all constants, and n≤10, k≥1.2, 0.3≥g≥0.15, 0.3≥f≥0.15; g, f, and k satisfy the relationship: k(1-f)+gf=1.

[0057] During the sterilization stage, high-temperature steam is introduced into the sealed fermentation tank body 1 and the sealed tank cover 2 to maintain the internal temperature of the fermentation tank body 1 not lower than 120°C. After 20-30 minutes of treatment, the introduction of high-temperature steam is stopped and the internal temperature of the fermentation tank body 1 is allowed to drop to room temperature.

[0058] In the initial feeding stage, sterile fermentation liquid with a sugar content of kP0 is introduced into the fermentation tank body 1, and then the bacterial liquid is introduced, and the stirring mechanism 3 is started at 75 rad / min to mix the fermentation liquid and the bacterial liquid until the total amount of the fermentation liquid and the bacterial liquid mixture is H0 and the sugar content is P0;

[0059] Any fermentation stage includes a bacterial growth stage and a bacterial fermentation stage. In the bacterial growth stage, the heat preservation jacket 4 maintains the temperature inside the fermentation tank 1 at 16-18°C. In the bacterial fermentation stage, the heat preservation jacket 4 maintains the temperature inside the fermentation tank 1 at 19-22°C. In the bacterial growth stage and the bacterial fermentation stage, the stirring speed of the stirring mechanism 3 is 30rad / min.

[0060] In any discharging stage, the stirring speed of the stirring mechanism 3 is 75 rad / min;

[0061] In any feeding stage, the stirring speed of the stirring mechanism 3 is 75 rad / min;

[0062] In the last discharging stage, the stirring speed of the stirring mechanism 3 is 75 rad / min.

[0063] Thus, when in use, the entire fermentation process of the entire intelligent control fermentation device includes a sterilization stage, an initial feeding stage, a first fermentation stage, a first discharge stage, a first feeding stage, ..., an nth fermentation stage, an nth discharge stage, an nth feeding stage, an n+1th fermentation stage and a final discharge stage;

[0064] The real-time sugar content P of the liquid obtained by the detection system 5 i , real-time height of liquid level H i , liquid real-time temperature Ti , distinguish all fermentations and accurately control the opening and closing states of the feed port 21, the opening and closing states of the discharge port 11, the stirring power of the stirring mechanism 3 and the temperature inside the fermentation tank body 1, so as to accurately control the progress of each process flow inside the fermentation tank 1, improve the fermentation effect, improve the fermentation efficiency, save the fermentation time of the entire batch, and improve the benefits of fermentation production.

[0065] For further information, please refer to Figure 1-Figure 3 A ventilation interface 22 is also provided on the top of the sealed tank cover 2, and a dissolved oxygen monitor is also provided inside the fermentation tank body 1. The ventilation interface 22 is externally connected to a ventilation filter and a ventilation pump. The ventilation interface 22 is also externally connected to a pressure relief valve, and the pressure relief valve is used to release the internal pressure of the fermentation tank body 1. The dissolved oxygen monitor can detect the dissolved oxygen content inside the fermentation tank body 1. The ventilation pump can exchange gas between the external air and the internal gas of the fermentation tank body 1 through the ventilation filter according to the change of the dissolved oxygen content inside the fermentation tank body 1.

[0066] Therefore, when in use, the internal pressure of the fermentation tank body 1 can be released through the pressure relief valve, and the ventilation pump can exchange the external air and the internal gas of the fermentation tank body 1 through the ventilation filter according to the change of the dissolved oxygen content inside the fermentation tank body 1. In actual use, the dissolved oxygen content inside the fermentation tank body 1 is maintained according to the optimal living conditions of the actual fermentation bacteria.

[0067] For further information, please refer to Figure 1-Figure 3 , Figure 7 The stirring mechanism 3 includes a stirring motor 31, a stirring connecting rod 32 and a stirring piece 33. The stirring motor 31 is fixed on the top of the sealed tank cover 2. The stirring motor 31 drives the stirring piece 33 to rotate through the stirring connecting rod 32. The stirring piece 33 includes a stirring outer frame 331 and a plurality of stirring blades 332. The stirring outer frame 331 is close to the inner wall of the fermentation tank body 1. When the stirring piece 33 is driven to rotate, the stirring outer frame 331 can stir the liquid inside the fermentation tank body 1 to flip in the horizontal direction, and the stirring blades 332 can stir the liquid inside the fermentation tank body 1 to flip in the vertical direction.

[0068] A discharge port 12 is also provided at the bottom of the fermentation tank body 1 , and the front end of the stirring outer frame 331 along the forward direction is inclined toward the rear end in the forward direction. When the stirring outer frame 331 rotates, it can scrape off the attachments on the inner wall of the fermentation tank body 1 and discharge them from the discharge port 12 .

[0069] Therefore, during use, through the setting of the stirring member 33, the stirring outer frame 331 can stir the liquid inside the fermentation tank body 1 to flip in the horizontal direction, and the stirring fan blades 332 can stir the liquid inside the fermentation tank body 1 to flip in the vertical direction, so as to fully mix the liquid inside the fermentation tank body 1; and in the last discharge stage, the discharge port 12 is opened, and the attachments on the inner wall of the fermentation tank body 1 are scraped off and discharged from the discharge port 12.

[0070] Embodiment 2:

[0071] A method for using an intelligent control fermentation device, using the intelligent control fermentation device as described in Example 1, the method comprising the following steps:

[0072] S1, sterilization stage, high-temperature steam is introduced into the sealed fermentation tank body 1 and the sealed tank cover 2 to maintain the internal temperature of the fermentation tank body 1 not lower than 120°C. After 20-30 minutes of treatment, the introduction of high-temperature steam is stopped, and the internal temperature of the fermentation tank body 1 is waited to drop to room temperature;

[0073] S2, the initial feeding stage, the sterile fermentation liquid with a sugar content of kP0 is introduced into the fermentation tank body 1, and then the bacterial liquid is introduced, and the stirring mechanism 3 is started at 75rad / min to mix the fermentation liquid and the bacterial liquid until the total amount of the fermentation liquid and the bacterial liquid mixture is H0 and the sugar content is P0;

[0074] S3, fermentation stage, including bacterial reproduction stage and bacterial fermentation stage;

[0075] S3.1, during the bacterial growth stage, the temperature inside the fermentation tank 1 is maintained at 16-18°C by the heat-insulating jacket 4, and the stirring speed of the stirring mechanism 3 is 30 rad / min until the sugar content P i Reduced from P0 to 1.5gP0;

[0076] S3.2, during the bacterial fermentation stage, the temperature inside the fermentation tank 1 is maintained at 19-22°C by the heat-insulating jacket 4, and the stirring speed of the stirring mechanism 3 is 30 rad / min until the sugar content P i From 1.5gP0 to gP0;

[0077] S4, discharge stage, the stirring speed of the stirring mechanism 3 is 75rad / min, the sugar content P i The liquid level H remains unchanged. i Reduce to fH0;

[0078] S5, feeding stage, the stirring speed of the stirring mechanism 3 is 75rad / min, and the fermentation liquid with a sugar content of kP0 is added, and the liquid level height H i Return to H0, sugar content P i Return to P0;

[0079] S6, looping through steps S3-S5 n times;

[0080] S7, the n+1th fermentation stage, which is the same as the fermentation stage of S3;

[0081] S8, the last discharging stage, the stirring speed of the stirring mechanism 3 is 75rad / min, and H iReduce to 0, and completely empty the fermentation tank 1;

[0082] S9, completely cleaning the fermentation tank body 1;

[0083] In the above steps, n, P0, H0, g, f, and k are all fixed values, and n≤10, k≥1.2, 0.3≥g≥0.15, 0.3≥f≥0.15;

[0084] g, f, and k satisfy the relationship: k(1-f)+gf=1.

[0085] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An intelligent controlled fermentation device, comprising a fermentation tank body (1), a sealed tank cover (2) and a stirring mechanism (3), wherein the fermentation tank body (1) and the sealed tank cover (2) are sealed, a feed port (21) is arranged at the top end of the sealed tank cover (2), and a liquid discharge port (11) is arranged at the bottom end of the fermentation tank body (1), and the stirring mechanism (3) can stir the inside of the fermentation tank body (1), characterized in that: The fermentation tank body (1) is provided with a heat-insulating jacket (4) outside, and the heat-insulating jacket (4) can perform temperature exchange and heat preservation on the inside of the fermentation tank body (1), and the temperature inside the fermentation tank body (1) can be adjusted by adjusting the power of the heat-insulating jacket (4); A detection system (5) is arranged inside the fermentation tank body (1), and the detection system (5) comprises a liquid level sensor (51), a temperature sensor (52) and a sugar content detection mechanism (53), wherein the liquid level sensor (51) is used to detect the height of the liquid level inside the fermentation tank body (1), the temperature sensor (52) is used to detect the temperature of the liquid inside the fermentation tank body (1), and the sugar content detection mechanism (53) is used to detect the sugar content of the liquid inside the fermentation tank body (1); A control system is also provided on the outside of the fermentation tank body (1), and the control system is capable of receiving detection data transmitted back by the detection system (5), wherein the detection data includes liquid level height data, temperature and liquid sugar content inside the fermentation tank body (1), and the control system is capable of performing calculations on the detection data to obtain the current fermentation progress inside the fermentation tank body (1); The feed port (21) and the liquid discharge port (11) are both provided with electromagnetic valves on their outer sides, and the opening and closing of the corresponding feed port (21) and the liquid discharge port (11) can be controlled by controlling the opening and closing of the electromagnetic valves; The control system can control the opening and closing state of the feed port (21), the opening and closing state of the liquid discharge port (11), the stirring power of the stirring mechanism (3) and the temperature inside the fermentation tank (1) according to changes in the fermentation process; The fermentation process includes a sterilization stage, an initial feeding stage, n cyclic fermentation stages and a final discharge stage; Each of the cyclic fermentation stages includes a fermentation stage, a discharge stage and a feeding stage; The real-time sugar content of the liquid obtained by the detection system (5) is P i , the real-time height of the liquid level is H i , the real-time temperature of the liquid is T i ; In the initial feeding stage, the sugar content of the mixed liquid of the fermentation liquid and the bacterial liquid entering is P0, and the total amount is H0. In the subsequent feeding stage, the sugar content of the fermentation liquid added is kP0; At any fermentation stage, P i From P0 to gP0; At any discharge stage, P i Remain unchanged, H i Reduce to fH0; In any feeding stage, fermentation liquid with sugar content kP0 is fed. i Return to H0, P i Return to P0; In the final discharge stage, H i Lowering to 0, completely emptying the fermentation tank (1); During the sterilization stage, high-temperature steam is introduced into the sealed fermentation tank body (1) and the sealed tank cover (2) to maintain the internal temperature of the fermentation tank body (1) not lower than 120° C. After 20-30 minutes of treatment, the introduction of high-temperature steam is stopped and the internal temperature of the fermentation tank body (1) is allowed to drop to room temperature. In the initial feeding stage, sterile fermentation liquid with a sugar content of kP0 is introduced into the fermentation tank body (1), and then bacterial liquid is introduced, and the stirring mechanism (3) is started to mix the fermentation liquid and bacterial liquid at 75 rad / min until the total amount of the fermentation liquid and bacterial liquid mixture is H0 and the sugar content is P0; Any fermentation stage includes a bacterial growth stage and a bacterial fermentation stage. In the bacterial growth stage, the heat preservation jacket (4) maintains the temperature inside the fermentation tank (1) at 16-18°C. In the bacterial fermentation stage, the heat preservation jacket (4) maintains the temperature inside the fermentation tank (1) at 19-22°C. In the bacterial growth stage and the bacterial fermentation stage, the stirring speed of the stirring mechanism (3) is 30 rad / min. At any discharging stage, the stirring speed of the stirring mechanism (3) is 75 rad / min; In any feeding stage, the stirring speed of the stirring mechanism (3) is 75 rad / min; In the last discharging stage, the stirring speed of the stirring mechanism (3) is 75 rad / min; The stirring mechanism (3) comprises a stirring motor (31), a stirring connecting rod (32) and a stirring member (33); the stirring motor (31) is fixed to the top end of the sealed can cover (2); the stirring motor (31) drives the stirring member (33) to rotate via the stirring connecting rod (32); The stirring member (33) comprises a stirring outer frame (331) and a plurality of stirring blades (332); the stirring outer frame (331) is in close contact with the inner wall of the fermentation tank body (1); when the stirring member (33) is driven to rotate, the stirring outer frame (331) can stir the liquid inside the fermentation tank body (1) to flip in the horizontal direction, and the stirring blades (332) can stir the liquid inside the fermentation tank body (1) to flip in the vertical direction.

2. The intelligent controlled fermentation device according to claim 1, characterized in that: The sugar content detection mechanism (53) comprises an auxiliary detection chamber (531), the auxiliary detection chamber (531) is arranged at one side of the bottom end of the fermentation tank body (1), and the auxiliary detection chamber (531) is communicated with the interior of the fermentation tank body (1), a solid filter plate (532) is arranged between the auxiliary detection chamber (531) and the fermentation tank body (1), and the liquid in the fermentation tank body (1) can pass through the solid filter plate (532) and enter the interior of the auxiliary detection chamber (531); A refractive index detection mechanism (533) is arranged inside the auxiliary detection cavity (531), and the refractive index detection mechanism (533) is capable of detecting the refractive index of the liquid inside the auxiliary detection cavity (531), and the sugar content of the liquid inside the auxiliary detection cavity (531) can be obtained based on the refractive index of the liquid.

3. The intelligent controlled fermentation device according to claim 2, characterized in that: The refractive index detection mechanism (533) includes a laser transmitter (5331), a laser receiver (5332) and an ultrasonic debubbler (5333); There is liquid between the laser transmitter (5331) and the laser receiver (5332) entering the auxiliary detection chamber (531) from the inside of the fermentation tank (1), and the ultrasonic defoamer (5333) is capable of emitting ultrasound to break bubbles in the liquid between the laser transmitter (5331) and the laser receiver (5332); The laser transmitter (5331) is capable of transmitting laser light to the laser receiver (5332), and the laser receiver (5332) is capable of receiving laser light, acquiring the refractive index of the liquid between the laser transmitter (5331) and the laser receiver (5332), and converting the sugar content of the liquid according to the refractive index of the liquid.

4. The intelligent controlled fermentation device according to claim 1, characterized in that: In any of the cyclic fermentation stages of a fermentation process, n, P0, H0, g, f, and k are all fixed values, and n≤10, k≥1.2, 0.3≥g≥0.15, and 0.3≥f≥0.15; g, f, and k satisfy the relationship: k(1-f)+gf=1.

5. The intelligent controlled fermentation device according to claim 1, characterized in that: The top of the sealed tank cover (2) is also provided with a ventilation interface (22), and the fermentation tank body (1) is also provided with a dissolved oxygen monitor. The ventilation interface (22) is externally connected to a ventilation filter and a ventilation pump. The ventilation interface (22) is also externally connected to a pressure relief valve, and the pressure relief valve is used to release the internal pressure of the fermentation tank body (1). The dissolved oxygen monitor can detect the dissolved oxygen content inside the fermentation tank body (1), and the ventilation pump can exchange gas between external air and the internal gas of the fermentation tank body (1) through the ventilation filter according to the change of the dissolved oxygen content inside the fermentation tank body (1).

6. The intelligent controlled fermentation device according to claim 1, characterized in that: The bottom end of the fermentation tank body (1) is also provided with a discharge port (12), and the front end of the stirring outer frame (331) is inclined toward the rear end in the forward direction. When the stirring outer frame (331) rotates, it can scrape off the attachments on the inner wall of the fermentation tank body (1) and discharge them from the discharge port (12).

7. A fermentation method, characterized in that: An intelligent controlled fermentation device as described in any one of claims 1 to 6 is used.

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