A povidone iodine solution for disinfecting chickens and a method for preparing the same

Abstract

The present application relates to the technical field of veterinary disinfectant, and proposes a povidone iodine solution for chicken disinfection and a preparation method thereof.The povidone iodine solution for chicken disinfection is composed of the following components in mass percentage: 1-10% of povidone iodine, 0.1-0.5% of potassium iodate, 5-20% of polyvinylpyrrolidone, 1-5% of patchouli extract, 2-10% of emollient, 0.1-0.5% of pH buffer, and the balance of water.The above technical solution solves the problem of poor disinfection effect and stability of povidone iodine in the prior art.The povidone iodine solution prepared by the present application is mild and non-irritating, has long-lasting effect and is less likely to produce drug resistance, and is suitable for chicken disinfection.

Description

Technical Field

[0001] The present invention relates to the technical field of veterinary disinfectants, and in particular to a povidone-iodine solution for disinfecting chickens and a preparation method thereof. Background Art

[0002] Spray disinfection of live chickens is a crucial step in chicken farms. It can directly kill pathogens lurking in the air, as well as pathogens lingering on the chicken's body and in the superficial respiratory tract, achieving comprehensive and thorough disinfection. Povidone-iodine, using povidone as a carrier, slowly releases free iodine to disrupt bacterial metabolism, inactivating microorganisms and achieving a killing effect. Povidone-iodine has varying degrees of bactericidal activity against most bacteria, fungi, and viruses. It is a highly effective, broad-spectrum iodine-containing disinfectant with low toxicity and non-corrosive properties, making it a common disinfectant for live chickens. However, povidone-iodine solutions are relatively unstable and prone to decomposition and sublimation during storage, reducing the effective iodine content and compromising their effectiveness. Furthermore, povidone-iodine is a topical medication. For chickens with or at risk of respiratory disease, inhalation of povidone-iodine solutions can induce respiratory mucosal edema, bleeding, and even pulmonary edema. Therefore, how to produce a disinfectant with significant disinfection effect, good stability and low irritation, especially for disinfecting sick chickens with or potentially suffering from respiratory diseases, is a problem that needs to be solved. Summary of the Invention

[0003] The invention provides a povidone-iodine solution for disinfecting live chickens and a preparation method thereof. The povidone-iodine solution has significant bactericidal and disinfecting effects, a long-lasting effect, is not prone to drug resistance, has strong stability, is mild and non-irritating, and is suitable for disinfecting live chickens.

[0004] The technical solutions of the present invention are as follows:

[0005] The present invention provides a povidone-iodine solution for disinfecting chickens, which is composed of the following components in percentage by mass:

[0006] Povidone-iodine 1%~10%, potassium iodate 0.1%~0.5%, polyvinylpyrrolidone 5%~20%, patchouli extract 1%~5%, emollient 2%~10%, pH buffer 0.1%~0.5%, and the balance is water.

[0007] As a further technical solution, the mass ratio of the patchouli extract to polyvinyl pyrrolidone is 1:4-6.

[0008] As a further technical solution, the mass ratio of the patchouli extract to polyvinyl pyrrolidone is 1:5.

[0009] In the present invention, by adjusting the mass ratio of patchouli extract and polyvinyl pyrrolidone, the steric hindrance of patchouli extract on povidone iodine is controlled, so that the patchouli extract and povidone iodine have a synergistic effect, and the disinfection effect and stability of the povidone iodine solution on chickens are further improved.

[0010] As a further technical solution, the polyvinyl pyrrolidone is one or more of PVPK15, PVPK30, and PVPK70.

[0011] As a further technical solution, when the polyvinyl pyrrolidone is PVP-K30 and PVP-K70, the mass ratio of PVP-K30 to PVP-K70 is 3:2~4:1.

[0012] In the present invention, when the polyvinyl pyrrolidone is PVPK30 and PVPK70, the mass ratio of PVPK30 to PVPK70 is 3:2-4:1, and the stability of the patchouli extract is improved by adjusting the system viscosity of the povidone-iodine solution, so that the patchouli extract and povidone-iodine have a synergistic effect, further improving the disinfection effect and stability of the povidone-iodine solution on chickens.

[0013] As a further technical solution, the pH buffer is at least one of sodium citrate, sodium acetate, and disodium hydrogen phosphate.

[0014] As a further technical solution, the emollient is propylene glycol and / or glycerin.

[0015] As a further technical solution, the preparation method of the patchouli extract comprises the following steps: distilling and extracting patchouli, collecting the extract, and obtaining the patchouli extract.

[0016] The present invention also provides a method for preparing the povidone-iodine solution for chicken disinfection, comprising the following steps:

[0017] S1, mixing the povidone iodine, potassium iodate and patchouli extract to obtain a mixture;

[0018] S2. The mixture is sequentially mixed with polyvinyl pyrrolidone and the remaining components to obtain a povidone-iodine solution.

[0019] The present invention also proposes the use of the povidone-iodine solution for disinfecting live chickens or the povidone-iodine solution prepared by the preparation method in disinfecting live chickens.

[0020] The preparation method of the povidone-iodine solution provided by the present invention can not only significantly improve the quality of the final product, but also has the advantages of a simple preparation method, strong quality controllability, product safety and high efficiency, and low production cost, and is suitable for industrial production.

[0021] The working principle and beneficial effects of the present application are:

[0022] In the present application, the povidone-iodine solution is added with the pachouli extract, the steric hindrance effect of the pachouli extract povidone-iodine is utilized, so that the release time of the effective iodine is prolonged, the stability of the povidone-iodine solution is enhanced, the drug efficacy of the povidone-iodine solution is more persistent, meanwhile, the polyvinylpyrrolidone serves as the carrier of the pachouli extract, and further improves the stability of the pachouli extract, the pachouli extract and the povidone-iodine synergistically improve the disinfection effect and the stability of the povidone-iodine solution on the chickens; the pachouli extract is a pure traditional Chinese medicine extract, has the effects of antibacterial, anti-inflammatory and antioxidant, can relieve the dysfunction of the mucosal barrier induced by uracil, inhibit the expression amount of occludin, reduce the mucosal permeability, promote the repair of the mucosa, synergistically improves the povidone-iodine solution, is not prone to drug resistance, is mild to the skin and does not irritate, has a high degree of bubbles when used, has a stronger affinity to the contacted objects, and has a more significant curative effect; the addition of the pachouli extract has a fragrance, so that the povidone-iodine solution prepared by the present application can enhance the comfort of the human and the animals when disinfecting the chickens. DETAILED DESCRIPTION

[0023] The technical solutions in the embodiments of the present application will be clearly and completely described below in combination with the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all the other embodiments obtained by those skilled in the art without creative labor also fall within the scope of protection of the present application.

[0024] In the following examples and comparative examples, the weight average molecular weights of PVP-K15, PVP-K30 and PVP-K70 are 8000, 38000 and 370000 respectively; the effective iodine content in the povidone-iodine is 9.0wt%-12.0wt%, and in the remaining examples and comparative examples, the operation methods used are all conventional operation methods, the equipment used is all conventional equipment, and the raw materials used are all commercially available, unless otherwise specified.

[0025] Example 1

[0026] The povidone-iodine solution for disinfecting the chickens is composed of the following components in mass percentage:

[0027] 1% of povidone-iodine, 0.1% of potassium iodate, 5% of PVP-K30, 5% of pachouli extract, 2% of propylene glycol, 0.3% of sodium citrate, and the balance of water;

[0028] The preparation method of the pachouli extract comprises the following steps: distillation extraction is performed on pachouli to collect the extract to obtain the pachouli extract;

[0029] The preparation method of povidone-iodine solution for chicken disinfection comprises the following steps:

[0030] S1. mixing povidone iodine, potassium iodate and patchouli extract to obtain a mixture;

[0031] S2. After mixing the mixture and PVP-K30, add water, propylene glycol, and sodium citrate and adjust the pH to 4 to obtain a povidone-iodine solution.

[0032] Example 2

[0033] Povidone-iodine solution for chicken disinfection is composed of the following components in percentage by mass:

[0034] Povidone iodine 5%, potassium iodate 0.3%, PVP-K30 15%, patchouli extract 5%, propylene glycol 7%, sodium citrate 0.3%, balance water;

[0035] The preparation method of patchouli extract comprises the following steps: distilling and extracting patchouli, collecting the extract, and obtaining the patchouli extract;

[0036] The preparation method of povidone-iodine solution for chicken disinfection comprises the following steps:

[0037] S1. mixing povidone iodine, potassium iodate and patchouli extract to obtain a mixture;

[0038] S2. After mixing the mixture and PVP-K30, add water, propylene glycol, and sodium citrate and adjust the pH to 5 to obtain a povidone-iodine solution.

[0039] Example 3

[0040] Povidone-iodine solution for chicken disinfection is composed of the following components in percentage by mass:

[0041] Povidone iodine 10%, potassium iodate 0.5%, PVP-K30 20%, patchouli extract 1%, glycerin 10%, sodium acetate 0.3%, balance water;

[0042] The preparation method of patchouli extract comprises the following steps: distilling and extracting patchouli, collecting the extract, and obtaining the patchouli extract;

[0043] The preparation method of povidone-iodine solution for chicken disinfection comprises the following steps:

[0044] S1. mixing povidone iodine, potassium iodate and patchouli extract to obtain a mixture;

[0045] S2. After mixing the mixture and PVP-K30, add water, glycerol, and sodium acetate, and adjust the pH to 4.5 to obtain a povidone-iodine solution.

[0046] Example 4

[0047] The difference between this embodiment and embodiment 2 is that the povidone-iodine solution for chicken disinfection is composed of the following components in percentage by mass:

[0048] Povidone iodine 5%, potassium iodate 0.3%, PVP-K15 15%, patchouli extract 5%, propylene glycol 7%, sodium citrate 0.3%, and the balance is water.

[0049] Example 5

[0050] The difference between this embodiment and embodiment 2 is that the povidone-iodine solution for chicken disinfection is composed of the following components in percentage by mass:

[0051] Povidone iodine 5%, potassium iodate 0.3%, PVP-K70 15%, patchouli extract 5%, propylene glycol 7%, sodium citrate 0.3%, and the balance is water.

[0052] Example 6

[0053] The difference between this embodiment and embodiment 2 is that the povidone-iodine solution for chicken disinfection is composed of the following components in percentage by mass:

[0054] Povidone iodine 5%, potassium iodate 0.3%, PVP-K30 6%, PVP-K70 9%, patchouli extract 5%, propylene glycol 7%, sodium citrate 0.3%, and the balance is water.

[0055] Example 7

[0056] The difference between this embodiment and embodiment 2 is that the povidone-iodine solution for chicken disinfection is composed of the following components in percentage by mass:

[0057] Povidone iodine 5%, potassium iodate 0.3%, PVP-K30 13.5%, PVP-K70 1.5%, patchouli extract 5%, propylene glycol 7%, sodium citrate 0.3%, and the balance is water.

[0058] Example 8

[0059] The difference between this embodiment and embodiment 2 is that the povidone-iodine solution for chicken disinfection is composed of the following components in percentage by mass:

[0060] Povidone iodine 5%, potassium iodate 0.3%, PVP-K30 9%, PVP-K70 6%, patchouli extract 5%, propylene glycol 7%, sodium citrate 0.3%, and the balance is water.

[0061] Example 9

[0062] The difference between this embodiment and embodiment 2 is that the povidone-iodine solution for chicken disinfection is composed of the following components in percentage by mass:

[0063] Povidone iodine 5%, potassium iodate 0.3%, PVP-K30 12%, PVP-K70 3%, patchouli extract 5%, propylene glycol 7%, sodium citrate 0.3%, and the balance is water.

[0064] Example 10

[0065] The difference between this embodiment and embodiment 2 is that the povidone-iodine solution for chicken disinfection is composed of the following components in percentage by mass:

[0066] Povidone iodine 5%, potassium iodate 0.3%, PVP-K30 12%, PVP-K15 3%, patchouli extract 5%, propylene glycol 7%, sodium citrate 0.3%, and the balance is water.

[0067] Example 11

[0068] The difference between this embodiment and embodiment 8 is that the povidone-iodine solution for chicken disinfection is composed of the following components in percentage by mass:

[0069] Povidone iodine 5%, potassium iodate 0.3%, PVP-K30 10.5%, PVP-K70 7%, patchouli extract 2.5%, emollient 7%, sodium citrate 0.3%, and the balance is water.

[0070] Example 12

[0071] The difference between this embodiment and embodiment 8 is that the povidone-iodine solution for chicken disinfection is composed of the following components in percentage by mass:

[0072] Povidone iodine 5%, potassium iodate 0.3%, PVP-K30 9.6%, PVP-K70 6.4%, patchouli extract 4%, emollient 7%, sodium citrate 0.3%, and the balance is water.

[0073] Example 13

[0074] The difference between this embodiment and embodiment 8 is that the povidone-iodine solution for chicken disinfection is composed of the following components in percentage by mass:

[0075] Povidone iodine 5%, potassium iodate 0.3%, PVP-K30 10.02%, PVP-K70 6.68%, patchouli extract 3.3%, emollient 7%, sodium citrate 0.3%, and the balance is water.

[0076] Example 14

[0077] The difference between this embodiment and embodiment 8 is that the povidone-iodine solution for chicken disinfection is composed of the following components in percentage by mass:

[0078] Povidone iodine 5%, potassium iodate 0.3%, PVP-K30 10.26%, PVP-K70 6.84%, patchouli extract 2.9%, emollient 7%, sodium citrate 0.3%, and the balance is water.

[0079] Comparative Example 1

[0080] The difference between this comparative example and Example 2 is that the povidone-iodine solution for chicken disinfection is composed of the following components in percentage by mass:

[0081] Povidone iodine 5%, potassium iodate 0.3%, PVP-K30 20%, emollient 7%, sodium citrate 0.3%, and the balance is water.

[0082] Set up control group 1 and control group 2 respectively:

[0083] Control group 1 used commercially available povidone-iodine solution as a reference substance;

[0084] Control group 2 used patchouli extract (prepared by the preparation method of patchouli extract in Example 2) as a reference substance.

[0085] 1. Detection of available iodine and free iodine:

[0086] According to the 2002 edition of the Disinfection Technical Specifications, a rapid assay was used to determine the retention rate of available iodine. 100 mL of povidone-iodine solution from Examples 1-14, Comparative Example 1, and Control 1 was placed in a brown glass bottle and stored in a dark, 80°C water bath for 15 hours. The available iodine and free iodine contents were then determined. The effective iodine retention rate was calculated as (available iodine content after 15 hours / available iodine content at day 0) × 100%. The test results are shown in Table 1 below:

[0087] Table 1 Test results of available iodine and free iodine

[0088]

[0089] It can be seen that in Examples 2 and 4 to 10, Examples 8 to 9 have a higher effective iodine retention rate and a lower free iodine content, indicating that the stability of PVP-K30 and PVP-K70 is increased by compounding, wherein the optimal mass ratio is 3:2 to 4:1. It can be seen from Comparative Example 1 and Control Group 1 that only the povidone-iodine solution is contained without the addition of patchouli extract, and the effective iodine retention rate is low, the free iodine content is high, and the stability is poor. It can be seen from Examples 11 to 14 that when the mass ratio of patchouli extract to polyvinyl pyrrolidone is within the range of 1:4 to 6, the effective iodine retention rate is the highest and the free iodine content is the lowest, wherein the optimal mass ratio is 1:5.

[0090] 2. Stability test:

[0091] The povidone-iodine solutions prepared in Examples 1 to 14, Comparative Example 1, and the control substance in Control Group 1 were separately packaged in glass bottles, tightly wrapped with black paper, and sealed. The bottles were then stored in a stability test chamber at 25°C and a relative humidity of 40°C for 18 months. The bottles were observed at the end of the 0th, 6th, 12th, and 18th months to check for caking and delamination, and the changes in the content were measured. The results are shown in Table 2.

[0092] Table 2 Available iodine loss rate

[0093]

[0094] In the long-term stability test, compared with the comparative example and the control group, the povidone-iodine solutions of Examples 1 to 14 were stable. By the end of 18 months, they all appeared as brownish-red uniform liquids, and the available iodine loss rate was less than 10%. However, comparative example 1 showed stratification after 12 months and precipitation by the end of 18 months, with an available iodine loss rate of 20.0%. Control group 1 showed stratification and precipitation after 12 months, and the available iodine loss rate was 24.2% by the end of 18 months.

[0095] 3. In vitro antibacterial test:

[0096] The KD method was used to conduct an in vitro antibacterial test to determine the antibacterial properties of the disinfectant. The specific method is as follows: Staphylococcus aureus, Escherichia coli, and Salmonella were evenly spread on the surface of a nutrient agar plate. The povidone-iodine solutions prepared in Examples 1 to 14 and Comparative Example 1, as well as the control substances in Control Groups 1 and 2, were diluted 5-fold with water. A 6mm diameter circular paper disc was then sterilized and dried under high pressure and then immersed in the dilution solution. After the disc was completely soaked, it was removed and placed on a nutrient agar plate. The plate was incubated at 37°C for 24 hours. The size of the inhibition zone was observed and recorded. The results are shown in Table 3:

[0097] Table 3 In vitro antibacterial test results

[0098]

[0099] In vitro antibacterial tests show that due to the synergistic effect of patchouli extract and povidone-iodine, Examples 1-14 achieved superior inhibition zones against Staphylococcus aureus, Escherichia coli, and Salmonella compared to Comparative Example 1 and Controls 1-2. Furthermore, in Examples 1-3, the antibacterial effect gradually increased with increasing povidone-iodine content. Example 13 exhibited the best antibacterial effect due to the addition of patchouli extract and the optimal povidone-iodine ratio.

[0100] 4. Bacterial resistance detection:

[0101] Escherichia coli was isolated from the chicken house and subjected to an in vitro antibacterial test. The chicken house was then disinfected in different areas. The povidone-iodine solutions prepared in Example 2 and Comparative Example 1 and the control substances of Control Groups 1 and 2 were diluted 500 times, and the chickens were disinfected once every two weeks. After 6 months, the in vitro antibacterial test was performed again. The results are shown in Table 4:

[0102] Table 4 Bacterial resistance

[0103]

[0104] The in vitro antibacterial test after 6 months showed that the diameter of the antibacterial zone of Example 2 of the present invention was larger and less likely to produce drug resistance, indicating that the patchouli extract and the povidone-iodine solution synergistically enhance the effect, are less likely to produce drug resistance, and are mild and non-irritating.

[0105] 5. Infectious disease virus killing experiment:

[0106] The povidone-iodine solutions prepared in Example 2 and Comparative Example 1 and the reference substances in Control Groups 1 and 2 were subjected to fowlpox virus killing experiments.

[0107] 5.1 IBV-M41 was diluted 1:10, 1:100, 1:200, 1:500, 1:1000, and 1:10000, respectively, and mixed with equal volumes of the 10-fold dilution from Example 2. The mixture was inoculated subcutaneously into 10-day-old rats, 20 per group, for a total of 120 rats. The rats were observed for 7 days after inoculation, and the number of rat deaths was recorded. The results are shown in Table 5.

[0108] Table 5 Results of the Infectious Disease Killing Experiment

[0109]

[0110] 5.2 IBV-M41 was diluted 1:500 and mixed with equal volumes of 10-fold dilutions of Example 2, Comparative Example 1, and Control Examples 2-3. The mixture was incubated at room temperature for 10 min and tested according to the method described in 5.1. The test results are recorded and shown in Table 6.

[0111] Table 6 Results of the Infectious Disease Killing Experiment

[0112]

[0113] As can be seen from Table 6, when comparative example 1 and control groups 1-2 were used, the number of deaths of rats was greater than that when example 2 was used, indicating that the povidone iodine and patchouli extract in the example coordinated and enhanced each other and had a better killing effect on bronchitis virus.

[0114] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A povidone-iodine solution for chicken disinfection, characterized in that: It is composed of the following components in percentage by mass: Povidone-iodine 1%-10%, potassium iodate 0.1%-0.5%, polyvinylpyrrolidone 5%-20%, patchouli extract 1%-5%, emollient 2%-10%, pH buffer 0.1%-0.5%, balance water; The polyvinyl pyrrolidone is one or more of PVP-K15, PVP-K30, and PVP-K70; The pH buffer is at least one of sodium citrate and sodium acetate; The emollient is propylene glycol or glycerin.

2. The povidone-iodine solution for chicken disinfection according to claim 1, wherein: The mass ratio of the patchouli extract to polyvinyl pyrrolidone is 1:4-6.

3. The povidone-iodine solution for chicken disinfection according to claim 2, wherein: The mass ratio of the patchouli extract to polyvinyl pyrrolidone is 1:

5.

4. The povidone-iodine solution for chicken disinfection according to claim 1, wherein: When the polyvinyl pyrrolidone is PVP-K30 and PVP-K70, the mass ratio of PVP-K30 to PVP-K70 is 3:2-4:

1.

5. The povidone-iodine solution for chicken disinfection according to claim 1, characterized in that: The preparation method of the patchouli extract comprises the following steps: taking patchouli for distillation extraction, collecting the extract, and obtaining the patchouli extract.

6. The method for preparing a povidone-iodine solution for chicken disinfection according to any one of claims 1 to 5, wherein: The following steps are involved: S1, mixing the povidone iodine, potassium iodate and patchouli extract to obtain a mixture; S2. The mixture is sequentially mixed with polyvinyl pyrrolidone and the remaining components to obtain a povidone-iodine solution.

7. Use of the povidone-iodine solution for disinfecting chickens according to any one of claims 1 to 5 or the povidone-iodine solution prepared by the preparation method according to claim 6 in preparing a drug for disinfecting chickens.

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