A microfluidic chip

Abstract

The present invention belongs to the field of microfluidic chip detection technology and provides a microfluidic chip having a first chamber and a second chamber interconnected with each other, as well as a detection chamber; the detection chamber is connected to both the first chamber and the second chamber via a first flow channel; the first chamber and the second chamber are both connected to an external supply system, the external supply system can selectively and automatically supply liquid to the first chamber and / or the second chamber; and the external supply system can selectively supply gas to the first chamber or the second chamber to drive the liquid to flow back and forth between the first chamber and the second chamber for mixing, and can also drive the liquid to flow to the detection chamber. The microfluidic chip can be placed in a spectrophotometer for measurement, and the amount of reagents required for measurement is greatly reduced.

Description

Technical Field

[0001] The present invention belongs to the technical field of microfluidic chip detection, and in particular relates to a microfluidic chip. Background Art

[0002] UV-Vis spectrophotometers can be used to perform highly sensitive quantitative and qualitative analysis of substances within cuvettes. Cuvettes of varying widths can also be used to accommodate different types and concentrations of substances.

[0003] Traditional UV-visible spectrophotometers use quartz cuvettes with frosted glass on both sides and high-transmittance glass at both ends of the optical path for light measurement. The cuvette itself, as a container, can only be used as a tool for holding liquids, and pipetting into the cuvette needs to be done manually. At the same time, if multiple liquids are mixed, they need to be manually mixed outside the cuvette and then pipetted into the cuvette. In addition, more reagents need to be added to the cuvette to reach the level that the spectrophotometer can measure. For example, about 6 ml of reagent sample needs to be added to a 20 ml cuvette. In addition, the waste liquid generated after the measurement is completed will also cause certain harm to the environment. Summary of the Invention

[0004] In view of the shortcomings of the prior art, the present invention aims to provide a microfluidic chip that can be placed in a spectrophotometer for measurement and greatly reduces the amount of reagents required for measurement.

[0005] To achieve this object, the present invention adopts the following technical solutions:

[0006] A microfluidic chip is provided with a first chamber and a second chamber that are interconnected, and a detection chamber;

[0007] The detection cavity is connected to both the first chamber and the second chamber via a first flow channel;

[0008] The first chamber and the second chamber are both connected to an external supply system, and the external supply system can selectively and automatically supply liquid into the first chamber and / or the second chamber;

[0009] Furthermore, the external supply system can selectively supply gas to the first chamber or the second chamber to drive the liquid to flow back and forth between the first chamber and the second chamber to mix, and can drive the liquid to flow to the detection chamber.

[0010] Preferably, the first chamber and the second chamber are arranged side by side and in parallel.

[0011] Preferably, the microfluidic chip comprises a chip body, a blocking block, and an optically transparent tape provided on the surfaces of the chip body and the blocking block;

[0012] The chip body and the blocking block are both made of black material;

[0013] The chip body is provided with a first groove, a second groove, a first flow channel groove and a detection groove. The first groove, the second groove and the first flow channel groove are covered with the optically transparent tape. The optically transparent tape is sealed with the chip body to form the first cavity, the second cavity and the first flow channel.

[0014] The blocking block is embedded in the detection groove, and the detection cavity is formed between the blocking block and the detection groove.

[0015] Preferably, a liquid channel and a gas channel communicating with the first chamber are provided on the chip body; and a communication port channel communicating with the second chamber is provided on the chip body.

[0016] The liquid channel and the gas channel are connected to an external supply system, and the communication port channel can communicate with the outside world.

[0017] Preferably, the liquid channel and the gas channel are arranged in parallel and in a flat shape at the top of the first chamber, and the communication port channel and the second outlet are arranged in parallel and in a flat shape at the top of the second chamber.

[0018] Preferably, the first flow channel includes a first horizontal section, a first vertical section, a second horizontal section and a second vertical section which are connected in sequence, the first horizontal section connects the first chamber and the second chamber, the second horizontal section is located above the first horizontal section, the first vertical section and the second vertical section are both located between the first horizontal section and the second horizontal section, and the second vertical section is connected to the detection chamber.

[0019] Preferably, the chip body and the blocking block are connected by any one of solvent bonding, gluing, hot pressing bonding and laser welding.

[0020] Preferably, the upper boundary surface of the detection groove includes a first inclined surface and a second inclined surface connected to each other, the lower end of the first inclined surface is connected to the lower end of the second inclined surface, and the angle between the first inclined surface and the second inclined surface ranges from 0 to 70°.

[0021] Preferably, a buffer cavity is provided on one side surface of the chip body, and the buffer cavity is connected to the detection cavity.

[0022] Preferably, a waste liquid flow channel is provided on the other side surface of the chip body, and the waste liquid flow channel is connected to the detection cavity.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] In the present invention, the external supply system can choose to automatically supply liquid to the first chamber and / or the second chamber. The liquid can be the same or different liquids, such as delivering a reagent to the first chamber and a sample to the second chamber, or delivering a sample to the first chamber and a reagent to the second chamber, or delivering a sample and a reagent to the first chamber at the same time, and the two flow back and forth between the first chamber and the second chamber to achieve mixing. The external supply system can also supply gas to the first chamber or the second chamber to drive the liquid to flow back and forth between the first chamber and the second chamber to achieve mixing. At the same time, the gas can also be used to drive the mixed liquid to flow through the first flow channel to the detection chamber.

[0025] The external supply system enables automatic liquid supply and mixing, reducing manual operations and improving work efficiency. Furthermore, liquid is automatically introduced and mixed between the first and second chambers, providing more precise control. This reduces the volume of liquid required for testing, further reducing the volume of the testing chamber and, consequently, the amount of sample used. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 Schematic diagram of the structure of the microfluidic chip of the present invention from a first angle;

[0027] Figure 2 2 is a schematic structural diagram of the microfluidic chip according to the present invention from a second angle;

[0028] Figure 3 This is a schematic structural diagram of the chip body in the present invention from a first angle;

[0029] Figure 4 A schematic structural diagram of the chip body according to the present invention from a second angle;

[0030] Figure 5 It is a front view of the chip body in the present invention.

[0031] Among them, 1, first chamber; 2, second chamber; 3, detection chamber; 31, first inclined surface; 32, second inclined surface; 33, inclined step; 331, first inclined section; 332, second inclined section; 4, first flow channel; 41, first horizontal section; 42, first vertical section; 43, second horizontal section; 44, second vertical section; 45, first arc section; 46, second arc section; 47, third arc section; 50, first mounting surface; 501, first inclined surface; 502, second inclined section; 503, first inclined section; 504, first inclined section; 505, first inclined section; 506, first inclined section; 507, first inclined section; 508, first inclined section; 509, first inclined section; 510, first inclined section; 511, first inclined section; 512, first inclined section; 513, first inclined section; 514, first inclined section; 515, first inclined section; 516, first inclined section; 517, second inclined section; 518, first inclined section; 519, first inclined section; 520, first inclined section; 521, first inclined section; 522, first inclined section; 523, first inclined section; 524, first inclined section; 525, first inclined section; 526, first inclined section; 527, second inclined section; 528, first inclined section; 529, first inclined section; 530, first inclined section; 531, first inclined section; 532, second inclined section; 02. Second inclined surface; 51. Second mounting surface; 52. First side surface; 53. Second side surface; 5. Chip body; 6. Sealing block; 7. Liquid channel; 8. Gas channel; 9. Connecting channel; 11. Buffer chamber; 12. Second flow channel; 13. Third flow channel; 14. Mixed liquid channel outlet; 15. Waste liquid flow channel; 151. First waste liquid vertical channel; 152. First waste liquid horizontal channel; 153. Second waste liquid vertical channel; 16. Waste liquid outlet channel. DETAILED DESCRIPTION

[0032] To make the objectives, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Generally, the components of the embodiments of the present invention described and shown in the drawings herein can be arranged and designed in various different configurations.

[0033] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the invention as claimed, but rather merely represents selected embodiments of the present invention. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without creative effort shall fall within the scope of protection of the present invention.

[0034] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, it does not need to be further defined or explained in subsequent drawings.

[0035] In the description of the present invention, it should be noted that the terms "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, or are the orientation or position relationship in which the product of the invention is usually placed when in use. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", "third", etc. are only used to distinguish the description and cannot be understood as indicating or implying relative importance. In the description of the present invention, unless otherwise specified, "multiple" means two or more.

[0036] In the description of the present invention, it should be noted that, unless otherwise specified or limited, the terms "disposed" and "connected" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integral connections; they can refer to mechanical connections or electrical connections. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on the specific circumstances.

[0037] In the present invention, unless otherwise expressly specified or limited, a first feature being "above" or "below" a second feature may include the first and second features being in direct contact, or may include the first and second features being in contact not directly but through another feature between them. Furthermore, a first feature being "above," "above," and "above" a second feature may include the first feature being directly above or obliquely above the second feature, or may simply mean that the first feature is higher in level than the second feature. A first feature being "below," "below," and "below" a second feature may include the first feature being directly below or obliquely below the second feature, or may simply mean that the first feature is lower in level than the second feature.

[0038] The following describes embodiments of the present invention in detail. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended only to explain the present invention and are not to be construed as limiting the present invention.

[0039] like Figure 1-Figure 5 As shown, this embodiment provides a microfluidic chip, which is used for measurement by an ultraviolet-visible spectrophotometer.

[0040] Specifically, the microfluidic chip is provided with a first chamber 1 and a second chamber 2, which are interconnected, as well as a detection chamber 3. The detection chamber 3 is connected to both the first chamber 1 and the second chamber 2 via a first flow channel 4. The first chamber 1 and the second chamber 2 are both connected to an external supply system, which can selectively and automatically supply liquid to the first chamber 1 and / or the second chamber 2.

[0041] Furthermore, the external supply system can selectively supply gas to the first chamber 1 or the second chamber 2 to drive the liquid to flow back and forth between the first chamber 1 and the second chamber 2 to mix, and can drive the liquid to flow to the detection chamber 3 .

[0042] The external supply system can automatically supply liquid to the first chamber 1 and / or the second chamber 2. The liquid can be the same or different liquids, such as delivering a reagent to the first chamber 1 and a sample to the second chamber 2, or delivering a sample to the first chamber 1 and a reagent to the second chamber 2, or delivering a sample and a reagent to the first chamber 1 at the same time, and the two flow back and forth between the first chamber 1 and the second chamber 2 to achieve mixing. The external supply system can also supply gas to the first chamber 1 or the second chamber 2 to drive the liquid to flow back and forth between the first chamber 1 and the second chamber 2 to achieve mixing. At the same time, the gas can also be used to drive the mixed liquid to flow through the first flow channel 4 to the detection chamber 3.

[0043] The external supply system enables automatic liquid supply and mixing, reducing manual operations and improving work efficiency. Furthermore, liquid is automatically introduced and mixed within the first and second chambers 1 and 2, providing more precise control. This reduces the volume of liquid required for testing, further reducing the capacity of the detection chamber 3 and, consequently, the amount of sample used.

[0044] Preferably, one of the first and second chambers 1, 2 is used to hold a sample, while the other is used to hold a reagent. Alternatively, the reagent and sample are delivered to the first chamber 1, and the mixed liquid flows back and forth between the first and second chambers 1, 2. The mixed liquid flows through the first flow channel 4 to the detection chamber 3, where the detection area of ​​the spectrophotometer is located. By reducing the capacity of the detection chamber 3, the amount of sample used can be reduced. This design can reduce the detection reagent volume to less than 500 μl, thereby reducing testing costs and minimizing environmental pollution.

[0045] Preferably, the first chamber 1 and the second chamber 2 are arranged in parallel. The first chamber 1 and the second chamber 2 have the same shape and structure. Figure 1 As shown, the first chamber 1 is located on the right side of the second chamber 2 as shown in the position in the figure.

[0046] In this embodiment, the sample and the reagent enter the first chamber 1 , and the second chamber 2 serves as a mixing chamber. The sample and the reagent flow back and forth between the first chamber 1 and the second chamber 2 to achieve mixing.

[0047] Preferably, the microfluidic chip includes a chip body 5 , a blocking block 6 , and an optically transparent tape provided on the surfaces of the chip body 5 and the blocking block 6 .

[0048] The chip body 5 is provided with a first groove, a second groove, a first flow channel groove, and a detection groove. The first groove, the second groove, and the first flow channel groove are covered with optically transparent tape, which seals the chip body 5 to form a first chamber 1, a second chamber 2, and a first flow channel 4. The chip body 5 and the blocking block 6 are both made of black material.

[0049] The blocking block 6 is embedded in the detection groove, and a detection cavity 3 is formed between the blocking block 6 and the detection groove.

[0050] In this embodiment, the chip body 5 and blocking block 6 of the microfluidic chip are both made of black plastic to reduce the impact of non-detection areas on light transmission. Preferably, the microfluidic chip is made of any one of PMMA, PC, PEEK, and PEI. These materials are highly plastic molecular materials and are resistant to acids and alkalis. More specifically, the microfluidic chip is made of any one of PMMA and PC. Commonly used microfluidic chips are made of PMMA.

[0051] The optically transparent tape covers the first groove, the second groove, and the first flow channel, forming the first chamber 1, the second chamber 2, and the first flow channel 4. It also seals these three structures to prevent leakage. Specifically, the optically transparent tape has high light transmittance, is non-sticky, compatible with biological reagents, chemically inert, and has low fluorescence. This high light transmittance refers to a light transmittance of 90% or more.

[0052] Preferably, the optically transparent tape covering the first groove, the second groove and the first flow channel groove is an integrated structure.

[0053] Preferably, the chip body 5 is provided with a liquid channel 7 and a gas channel 8 communicating with the first chamber 1 ; and the chip body 5 is provided with a communication port channel 9 communicating with the second chamber 2 .

[0054] Both the liquid channel 7 and the gas channel 8 are connected to the external supply system, and the connecting port channel 9 can be connected to the outside atmosphere. The external supply system can automatically transport samples and reagents to the first chamber 1 through the liquid channel 7. At this time, the liquid channel 7 is open, the gas channel 8 is open, and the connecting port channel 9 is closed, or the liquid channel 7 is open, the gas channel 8 is closed, and the connecting port 9 is open. The external supply system can also introduce gas into the first chamber 1 and the second chamber 2 through the gas channel 8. At this time, the liquid channel 7 is closed, the connecting port channel 9 is open, and the external supply system transports gas to the first chamber 1 through the gas channel 8 to drive the mixed liquid to move back and forth between the first chamber 1 and the second chamber 2. After repeated pumping, the mixed liquid flows back and forth in the first chamber 1 and the second chamber 2.

[0055] Specifically, the drive structure can include a first and second drive pipes, with a liquid channel 7 connected to the drive via the first pipe, and a gas channel 8 connected to the drive via the second pipe. The drive can contain liquid and deliver a predetermined amount of liquid into the first chamber 1 via the pipe and liquid channel 7. Simultaneously, it can also supply gas to the first chamber 1 and the second chamber 2 via the second pipe and gas channel 8.

[0056] Further preferably, the liquid channel 7 and the gas channel 8 are arranged in parallel and in a flat shape at the top of the first chamber 1 , and the communication port channel 9 is arranged at the top of the second chamber 2 .

[0057] Preferably, the liquid channel 7, the gas channel 8, and the connecting port channel 9 are all circular channels. Specifically, the liquid channel 7 includes a first section and a second section that are interconnected. The first section and the second section are both circular channels. The second section is connected to the first chamber, and the diameter of the second section is smaller than that of the first section. Reducing the amount of sample passing through the second section facilitates controlling the injection volume of the sample. Generally, the sample enters the liquid channel 7 drop by drop, thereby improving the accuracy of liquid supply to the first chamber 1. If the diameter of the second section of the liquid channel 7 is too large, the volume of each drop of sample will also increase. Preferably, the diameter of the gas channel 8 is larger than the diameter of the first section of the liquid channel 7 in order to facilitate the processing of the gas channel 8 and to facilitate air circulation to reduce the risk of blockage. During mixing, the liquid channel 7 is closed, the connecting port 9 is opened to connect to the air, and the gas channel 8 is connected to the syringe. The reagent and sample are drawn back and forth, and the back and forth movement between the first chamber 1 and the second chamber 2 achieves mixing.

[0058] Since the cost of samples is relatively high, the amount of samples used is minimized during the detection process. Preferably, a second channel communicating with the second chamber 2 is provided at the bottom of the first chamber 1 so that the sample liquid in the first chamber 1 can be fully mixed with the reagent in the second chamber 2.

[0059] Preferably, the first flow channel 4 includes a first horizontal section 41, a first vertical section 42, a second horizontal section 43, and a second vertical section 44, which are connected in sequence. The first horizontal section 41 connects the first chamber 1 and the second chamber 2. The second horizontal section 43 is located above the first horizontal section 41. The first vertical section 42 and the second vertical section 44 are both located between the first horizontal section 41 and the second horizontal section 43. The second vertical section 44 connects to the detection chamber 3. The curved channel formed by the first vertical section 42, the second horizontal section 43, and the second vertical section 44 in the first chamber 1 and the second chamber 2 prevents liquid from entering the chamber, causing a large impact and generating bubbles, which may affect the detection results.

[0060] Further preferably, the first horizontal section 41 and the first vertical section 42 are connected via a first arc section 45, the first vertical section 42 and the second horizontal section 43 are connected via a second arc section 46, and the second horizontal section 43 and the second vertical section 44 are connected via a third arc section 47. The purpose of providing the above-mentioned arc sections is to facilitate the mixed liquid to smoothly pass through the first flow channel 4 into the detection chamber 3, thereby reducing resistance.

[0061] There is no specific limitation on the height of the first vertical section 42 and the second vertical section 44 , as long as the mixed liquid can pass through the channel smoothly without generating bubbles.

[0062] Preferably, the width of the first flow channel 4 is in the range of 0.1 mm to 0.3 mm, and the width refers to the dimension in the horizontal direction.

[0063] Preferably, the first horizontal section 41 and the first vertical section 42 are connected via a first arc section 45, and the minimum distance between the edge of the first arc section 45 and the edge of the detection groove is 1 mm to 3 mm, so as to achieve complete sealing and prevent leakage.

[0064] Preferably, the chip body 5 and the blocking block 6 are connected by any one of solvent bonding, adhesive bonding, thermal compression bonding and laser welding.

[0065] Solvent bonding is a commonly used method for bonding thermoplastics. "Applying a solvent to a thermoplastic softens the polymer, and the pressure causes the polymer chains to diffuse into each other at the bond line. When the solvent evaporates, a fully bonded bond line is left behind. An advantage of solvent bonding over other polymer bonding methods is that bonding typically occurs below the glass transition temperature of the polymer. Solvent bonding differs from adhesive bonding in that no solvent is permanently added to the substrates being bonded. Solvent bonding differs from other plastic welding processes in that heat energy is generated by a chemical reaction between the solvent and the thermoplastic, which cools as the solvent evaporates." The solvent used varies depending on the specific thermoplastic, such as chloroform for PMMA or cyclohexane for COC, or ultrasonic bonding can be used to achieve a leak-proof bond.

[0066] Thermocompression bonding is a process used to seal microfluidic chips by heating a thermoplastic material to near or above its glass transition temperature (Tg) while simultaneously applying pressure. When heated near or above its Tg, the thermoplastic becomes rubbery and can deform under applied pressure. Following heating and pressure, the polymer chains "fuse" together through interdiffusion at the bonding interface.

[0067] Laser welding is another localized welding method used to bond thermoplastic substrates. Laser transmission welding (LTW) involves localized heating at the interface of the two thermoplastic materials to be bonded. Therefore, one of the plastics must be optically transparent to the laser radiation, while the other must be absorptive. The laser energy absorbed by this material induces vibrations in the electron bonds, which then transfer heat to the surroundings through convection and radiation. When heated to a temperature above the transition temperature (Tg) to reach the melting point, a weld is formed while applying pressure to increase the mating contact force.

[0068] Preferably, optically transparent tape is applied to the surface of the sealing member to seal the gap between the sealing member 6 and the chip body 5, further enhancing the seal and preventing leakage. Preferably, the optically transparent tape is integrated with the optically transparent tapes covering the first chamber 1, the second chamber 2, and the first flow channel groove to ensure overall sealing.

[0069] Preferably, the detection groove, the first groove, the second groove, and the first flow channel groove are provided on the same surface of the chip body 5 so as to be covered and sealed with an integrated optically transparent tape.

[0070] Preferably, the upper boundary surface of the detection tank includes a first inclined surface 31 and a second inclined surface 32 connected to each other. The lower end of the first inclined surface 31 is connected to the lower end of the second inclined surface 32, and the connecting line between the two is the lowest point of the upper boundary surface of the detection tank. The angle between the first inclined surface 31 and the second inclined surface 32 ranges from 0° to 70°.

[0071] The reason for setting an angle between the first inclined surface 31 and the second inclined surface 32 is that the mixed liquid flows into the detection chamber 3 through the first flow channel 4. In the process of entering the detection chamber 3, the bubbles generated will move upward, and the mixed liquid will preferentially fill the lower part of the detection chamber 3. Because of the provision of the first inclined surface 31, the bubbles will remain outside the detection area and will not interfere with the detection.

[0072] Preferably, the horizontal dimension of the first inclined surface 31 is 1 / 3-2 / 3 of the horizontal dimension of the chip body 5, so that there is enough space to accommodate bubbles. Further preferably, the horizontal dimension of the first inclined surface 31 is 1 / 3 of the horizontal dimension of the chip body 5. Bubbles passing through the first flow channel 4 can quickly enter the space between the first inclined surface 31 and the horizontal plane as soon as they enter the detection chamber 3, and are blocked by the turning point between the first inclined surface 31 and the second inclined surface 32. They will not continue to move forward to the second inclined surface 32, so that they can be quickly detected and work efficiency is improved.

[0073] In other embodiments, the horizontal dimension of the first inclined surface 31 is 2 / 3 of the horizontal dimension of the chip body 5. If the space between the first inclined surface 31 and the horizontal surface is too large, bubbles cannot accumulate stably in the space and will move back and forth, affecting the detection effect.

[0074] The bottom of the detection chamber 3 is provided with an inclined step 33, which gradually slopes upward from the inlet end where the liquid enters the detection chamber to the end opposite the inlet end. The inclined step 33 is provided so that after the test is completed, the liquid in the detection chamber 3 is gradually drawn out and flows back to the inlet end of the detection chamber along the inclined step 33.

[0075] In other embodiments, the inclined step 33 includes a first inclined section 331 and a second inclined section 332 , and the inclination angle between the first inclined section 331 and the horizontal plane is greater than the inclination angle between the second inclined section 332 and the horizontal plane.

[0076] Preferably, a first mounting surface 50 is provided on the upper end surface of the detection slot. The first mounting surface 50 is located above the first inclined surface 31 and the second inclined surface 32. The first mounting surface 50 is connected to the first side surface 52 between the first inclined surface 31 and the second side surface 53 between the second inclined surface 32. A first step is formed between the first mounting surface 50, the first inclined surface 31, the second inclined surface 32, the first side surface 52, and the second side surface 53. Specifically, the top surface of the blocking block 6 contacts the first mounting surface 50, and the inner side surface of the upper end of the blocking block 6 contacts the first side surface 52 and the second side surface 53.

[0077] The first mounting surface 50 includes a first inclined surface 501 and a second inclined surface 502. The first inclined surface 501 is parallel to the first inclined surface 31, and the second inclined surface 502 is parallel to the second inclined surface 32. In other embodiments, the first mounting surface 50 can also be a horizontal plane.

[0078] A second mounting surface 51 is provided at the lower end of the detection slot. This second mounting surface 51 is located below the inclined surface of the inclined step 33. The second mounting surface 51 and the side surface of the inclined step 33 form a second step. The inner side surface of the lower end of the blocking block 6 contacts the side surface of the inclined step 33, and the bottom surface of the lower end of the blocking block 6 contacts the second mounting surface 51. The space between the blocking block 6 and the detection slot forms a detection cavity. Preferably, the second mounting surface 51 is a horizontally disposed plane.

[0079] Preferably, a buffer cavity 11 is provided on one side surface of the chip body 5, and the buffer cavity 11 is connected to the detection cavity 3. When the mixed liquid in the detection cavity 3 is filled, part of the reagent will be stored in the buffer cavity 11 to prevent the reagent from overflowing.

[0080] Preferably, a second flow channel 12 is provided on the chip body 5, and the detection chamber 3 is connected to the buffer chamber 11 through the second flow channel 12. The mixed liquid flows into the buffer chamber 11 through the second flow channel 12. The second flow channel 12 is provided to prevent the mixed liquid in the buffer chamber 11 from quickly flowing back to the detection chamber 3, causing impact on the liquid in the detection chamber 3 and affecting the detection result.

[0081] Preferably, the chip body 5 defines a third flow channel 13 communicating with the buffer chamber 11, and a mixed liquid channel opening 14 communicating with the third flow channel 13. The third flow channel 13 is positioned above the buffer chamber 11, and the mixed liquid channel opening 14 is connected to an external valve. After the mixed liquid in the buffer chamber 11 is filled, it flows through the third flow channel 13 to the mixed liquid channel opening 14, where it is released to the outside through the external valve.

[0082] Further preferably, the third flow channel 13 and the mixed liquid channel port 14 are both connected through a channel provided on the chip body 5 .

[0083] Preferably, the second flow channel 12 , the buffer chamber 11 and the third flow channel 13 are all arranged on the side of the chip body 5 close to the first chamber 1 , so that the mixed liquid passing through the detection chamber 3 can fill the detection chamber 3 .

[0084] Preferably, the chip body 5 is provided with a second flow channel groove, a buffer cavity groove, and a third flow channel groove. The entire side where the second flow channel 12, the buffer cavity 11, and the third flow channel 13 are located is covered with a high-transmittance microfluidic tape to seal the second flow channel groove, the buffer cavity groove, and the third flow channel groove on the side to form the second flow channel 12, the buffer cavity 11, and the third flow channel 13. At the same time, the opening at the right end of the detection cavity is sealed.

[0085] Preferably, a waste liquid flow channel 15 is provided on the other side of the chip body 5, and the waste liquid flow channel 15 is connected to the detection chamber 3. When the detection is completed, the mixed liquid is extracted from the waste liquid flow channel 15 to clean the microfluidic chip, and then the process of mixing reagents and samples is used to clean the microfluidic chip.

[0086] Preferably, the waste liquid flow channel 15 includes a first waste liquid vertical channel 151 , a first waste liquid horizontal channel 152 , a second waste liquid vertical channel 153 , and a waste liquid outlet channel 16 connected to the outside, which are connected in sequence.

[0087] The first waste liquid vertical channel 151 and the second waste liquid vertical channel 153 are parallel and perpendicular to the first waste liquid horizontal channel 152. The first waste liquid vertical channel 151 is connected to the detection chamber 3 and is located below the detection chamber 3. The waste liquid outlet channel 16 is located above the first chamber 1 and the second chamber 2. The waste liquid outlet channel 16 is connected to the outside, and the waste liquid after detection in the detection chamber 3 can be extracted through the external suction structure to clean the inside of the microfluidic chip.

[0088] The first waste liquid horizontal channel 152 is provided to prevent the waste liquid from flowing back into the detection chamber 3 and will not affect the detection of the mixed liquid in the detection chamber 3.

[0089] Similarly, the side surface of the chip body 1 is provided with a first vertical waste liquid channel groove, a first horizontal waste liquid channel groove, and a second vertical waste liquid channel groove. High-permeability microfluidic tape is applied to the entire surface of the first waste liquid channel 15 to seal the first vertical waste liquid channel groove, the first horizontal waste liquid channel groove, and the second vertical waste liquid channel groove, forming a first vertical waste liquid channel 151, a first horizontal waste liquid channel 152, and a second vertical waste liquid channel 153. At the same time, the opening at the left end of the detection cavity is sealed.

[0090] Obviously, the above embodiments of the present invention are merely examples for the purpose of clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Those skilled in the art will appreciate that other variations or modifications can be made based on the above description. It is not necessary and impossible to enumerate all embodiments here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the claims of the present invention.

Claims

1. A microfluidic chip, characterized in that: The microfluidic chip is provided with a first chamber (1) and a second chamber (2) that are interconnected, as well as a detection chamber (3); The detection cavity (3) is in communication with both the first chamber (1) and the second chamber (2) via a first flow channel (4); The first chamber (1) and the second chamber (2) are both connected to an external supply system, and the external supply system can automatically supply liquid into the first chamber (1) and / or the second chamber (2); Furthermore, the external supply system is capable of supplying gas to the first chamber (1) or the second chamber (2) to drive the liquid to flow back and forth between the first chamber (1) and the second chamber (2) to mix, and is capable of driving the liquid to flow to the detection chamber (3); The microfluidic chip comprises a chip body (5), a detection groove is provided on the chip body (5), and the detection cavity (3) is formed in the detection groove; The detection slot comprises a first inclined surface (31) and a second inclined surface (32) connected to each other, wherein the lower end of the first inclined surface (31) is connected to the lower end of the second inclined surface (32); The bubbles passing through the first flow channel (4) enter the detection chamber (3) and enter the space between the first inclined surface (31) and the horizontal surface, and the turning point between the first inclined surface (31) and the second inclined surface (32) prevents the bubbles from moving toward the second inclined surface (32); The first flow channel (4) comprises a first horizontal section (41), a first vertical section (42), a second horizontal section (43) and a second vertical section (44) which are connected in sequence, wherein the first horizontal section (41) is connected to the first chamber (1) and the second chamber (2), the second horizontal section (43) is located above the first horizontal section (41), the first vertical section (42) and the second vertical section (44) are both located between the first horizontal section (41) and the second horizontal section (43), and the second vertical section (44) is connected to the detection chamber (3).

2. The microfluidic chip according to claim 1, characterized in that The first chamber (1) and the second chamber (2) are arranged in parallel.

3. The microfluidic chip according to claim 1 or 2, characterized in that: The microfluidic chip comprises a blocking block (6), and an optically transparent adhesive tape arranged on the surface of the chip body (5) and the blocking block (6); The chip body (5) and the blocking block (6) are both made of black material; The chip body (5) is further provided with a first groove, a second groove, and a first flow channel groove; the first groove, the second groove, and the first flow channel groove are covered with the optically transparent adhesive tape; the optically transparent adhesive tape and the chip body (5) are sealed to form the first cavity (1), the second cavity (2), and the first flow channel (4); The blocking block (6) is embedded in the detection groove, and the detection cavity (3) is formed between the blocking block (6) and the detection groove.

4. The microfluidic chip according to claim 3, characterized in that The chip body (5) is provided with a liquid channel (7) and a gas channel (8) communicating with the first chamber (1); and the chip body (5) is provided with a communication port channel (9) communicating with the second chamber (2); The liquid channel (7) and the gas channel (8) are connected to an external supply system, and the communication port channel (9) can communicate with the outside world.

5. The microfluidic chip according to claim 4, characterized in that: The liquid channel (7) and the gas channel (8) are arranged in parallel and in a flat shape at the top of the first chamber (1), and the communication port channel (9) is arranged at the top of the second chamber (2).

6. The microfluidic chip according to claim 3, characterized in that The chip body (5) and the blocking block (6) are connected by any one of solvent bonding, gluing, hot pressing bonding and laser welding.

7. The microfluidic chip according to claim 1, characterized in that The included angle between the first inclined surface (31) and the second inclined surface (32) ranges from 0° to 70°.

8. The microfluidic chip according to claim 3, characterized in that: A buffer cavity (11) is provided on one side surface of the chip body (5), and the buffer cavity (11) is connected to the detection cavity (3).

9. The microfluidic chip according to claim 3, characterized in that: A waste liquid flow channel (15) is provided on the other side of the chip body (5), and the waste liquid flow channel (15) is connected to the detection cavity (3).

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