Method for biosynthesizing type VIII collagen, a structural material for the human body

Humanized VIII collagen is produced through genetic engineering technology, and C8a and C8c polypeptides with cell adhesion activity and triple helical structure are expressed and purified by polypeptide recombination technology, solving the problem of insufficient water solubility and processability of animal-derived collagen. , achieving efficient collagen biosynthesis.

CN118388629BActive Publication Date: 2025-02-11SHANXI JINBO BIO PHARMACEUTICAL CO LTD
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Patent Information

Application Number
CN202410467063.1
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-07-18
Publication Date
2025-02-11
Estimated Expiration
2043-07-18

AI Technical Summary

Technical Problem

In the prior art, collagen mainly comes from animal tissues, resulting in poor water solubility and weak processability, limiting its application in medicine and industry.

Method used

Humanized VIII collagen was produced by genetic engineering technology, and C8a and C8c polypeptides with cell adhesion activity and triple helical structure were expressed and purified using polypeptide recombination technology.

Benefits of technology

The efficient expression and purification of collagen is achieved, the problem of insufficient water solubility and processability of animal-derived collagen is overcome, and biosynthetic materials are more suitable for medical and industrial applications.

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Abstract

A method for biosynthesizing type VIII collagen, a structural material for the human body, is provided. The present disclosure provides a polypeptide comprising one or more repeating units, wherein the repeating units are directly or connected via a linker, and the repeating units comprise an amino acid sequence or a variant thereof selected from the group consisting of SEQ ID NO: 1, 4, 7, 10, 13, 16, 19, 22, 25 or 28. The polypeptide can be used for biological dressings, human bionic materials or plastic surgery materials. The method herein utilizes genetic engineering technology to produce type VIII collagen, overcoming the defects of the prior art.
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Citation Information

Patent Citations

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