A moisturizing and whitening cream and preparation method thereof

Abstract

The invention discloses a moisturizing and whitening cream and a preparation method thereof, aiming to provide a moisturizing and whitening cream which is mild and non-irritating, can inhibit or reduce melanin in all directions, accelerate skin metabolism, promote melanin shedding, reduce ultraviolet radiation, replenish moisture required by the skin, and can effectively improve microcirculation; the technical scheme thereof is: 1-10% oil, 2-8% polyol, 0.5-2% protease glucan, 0.2-2% mannan, 3-7% tocopherol, 2-5% cetearyl alcohol, 1-5% rice fermentation product filtrate, 0.5-1.2% black mulberry leaf extract, 1‑3% camellia leaf extract, 0.5‑1% plantain fruit extract, 0.1‑0.3% liquorice root extract, 0.2‑0.8% purslane extract, 0.2‑1% disodium cocoyl ethylenediamine PEG‑15 disulfate, 1‑2.5% soy lecithin, 0.5‑1.2% poloxamer, 0.1‑0.5% tetrasodium glutamate diacetate, 0.1‑0.3% allantoin, 0.1‑0.5% carbomer, 0.2‑1% dibutyl lauroyl glutamine, 0.5‑1% preservative, and appropriate amount of water; belonging to the technical field of cosmetics.

Description

Technical Field

[0001] The invention relates to a whitening cream, in particular to a moisturizing whitening cream. The invention also relates to a preparation method of the moisturizing whitening cream and belongs to the technical field of cosmetics. Background Art

[0002] Skin color is determined by the content and distribution of melanin. Melanocytes are glandular cells located in the basal cell layer of the epidermis and the hair follicles of the skin. They can synthesize and secrete melanin. Melanin is formed by the reaction of tyrosine in immature melanin and tyrosinase. This enzyme can convert tyrosine into dopachrome, which then goes through a series of steps to become melanin. Excessive melanin formation leads to dull skin and the formation of spots. Therefore, reducing the production of melanin has a better effect on skin whitening.

[0003] Its whitening mechanism can be mainly divided into the following categories:

[0004] 1. Controlling or inhibiting the enzymes required in each link of the melanin production process is the main way to reduce the formation of melanin. Therefore, inhibiting the enzymes required in each link of the melanin production process in cells can hinder the production of melanin and effectively reduce the amount of melanin.

[0005] 2. Reduce the synthesized melanin or inhibit the oxidation of DOPA itself. The reducing agent can reduce the oxidation products in the melanin production process, and block the production of melanin by continuously reducing the oxidation products. At the same time, the reducing agent can also dilute melanin and inhibit the production of DOPA oxidized product dopaquinone. In addition, the reducing agent can also inhibit the free radical activity caused by ultraviolet rays, haze, etc., thereby slowing down the oxidation of skin collagen and other factors that cause aging and dull skin color.

[0006] 3. Inhibit the transport of melanin

[0007] For the melanin that has been produced, if it cannot be smoothly transported to the stratum corneum, it will not cause visual "darkening". Therefore, by inhibiting the transport of melanin, it cannot reach the surface of the skin and will not be seen by the naked eye.

[0008] 4. Accelerate skin metabolism and stratum corneum shedding

[0009] Even if the melanin that has been produced successfully reaches the surface of the skin, it will speed up the skin's metabolism and accelerate the shedding of the stratum corneum, thereby improving skin color.

[0010] In addition, reducing the negative impact of exogenous factors such as ultraviolet rays on the physiological process of melanin formation can also inhibit skin darkening, thereby achieving a skin whitening effect.

[0011] Therefore, the development of whitening products mainly revolves around controlling or inhibiting the enzymes required in each link of the melanin production process; reducing the synthesized melanin or inhibiting the self-oxidation of DOPA; inhibiting the transport of melanin, accelerating skin metabolism and stratum corneum shedding, and reducing exogenous factors. However, most of the products claiming to be whitening are not actually effective. The possible reason is that the inhibition principle is single, and thus cannot solve the spots caused by various reasons. Some products even add some illegal ingredients to achieve the purpose of rapid whitening, causing irreversible damage to the skin. Summary of the invention

[0012] In view of the above problems, the purpose of the present invention is to provide a moisturizing and whitening cream that is mild and non-irritating, can inhibit or reduce melanin in all directions, accelerate skin metabolism, promote melanin shedding, reduce ultraviolet radiation, replenish the moisture needed by the skin, and effectively improve microcirculation.

[0013] Another object of the present invention is to provide a method for preparing the moisturizing and whitening cream.

[0014] In order to achieve the above object, the first technical solution provided by the present invention is as follows:

[0015] A moisturizing and whitening cream comprises the following components in percentage by weight: 1-10% of oil, 2-8% of polyol, 0.5-2% of protease dextran, 0.2-2% of mannan, 3-7% of tocopherol, 2-5% of cetearyl alcohol, 1-5% of rice fermentation product filtrate, 0.5-1.2% of black mulberry leaf extract, 1-3% of camellia sinensis leaf extract, 0.5-1% of plantain fruit extract, 0.1-0.3% of glycyrrhiza inflata root extract, 0.2-0.8% of purslane extract, 0.2-1% of disodium dicocoyl ethylenediamine PEG-15 disulfate, 1-2.5% of soybean lecithin, 0.5-1.2% of poloxamer, 0.1-0.5% of tetrasodium glutamate diacetate, 0.1-0.3% of allantoin, 0.1-0.5% of carbomer, 0.2-1% of dibutyl lauroyl glutamine, 0.5-1% of preservative and an appropriate amount of water.

[0016] Furthermore, the above-mentioned moisturizing and whitening cream further comprises 0.5-1.0% of Sanguisorba officinalis root extract.

[0017] Furthermore, the above-mentioned moisturizing and whitening cream also includes 0.05-0.12% of Rhizoma Corydalis leaf extract and 0.1-1.0% of cabbage leaf extract.

[0018] Furthermore, the poloxamer in the above-mentioned moisturizing and whitening cream is poloxamer 124 or poloxamer 184 or poloxamer 188.

[0019] Furthermore, the oil described in the above-mentioned moisturizing and whitening cream is a compound of tea seed oil, rose essential oil and tomato seed oil in a mass ratio of 2:2:1.

[0020] Furthermore, the preservative in the above-mentioned moisturizing and whitening cream is a complex of phenoxyethanol and ethylhexylglycerin.

[0021] Furthermore, the polyol described in the above-mentioned moisturizing and whitening cream includes at least one of 1,3-propylene glycol, glycerol, 1,2-pentanediol, 1,2-hexanediol, and ethylhexylglycerol.

[0022] Furthermore, the black mulberry leaf extract in the above-mentioned moisturizing and whitening cream is prepared by the following method:

[0023] 1) ultrafinely grinding black mulberry leaves to 80-100 μm, adding 2-4 times the mass of water, soaking at room temperature for 12-36 hours, centrifuging and separating to obtain a black mulberry leaf soaking extract;

[0024] 2) primary fermentation: adding sodium dihydrogen phosphate-citric acid buffer solution to the black mulberry leaf soaking extract of step 1) to adjust the pH value to neutral, then adding 1.0-2.0% composite extraction enzyme to the black mulberry leaf soaking extract, fermenting and stirring at 35-40° C. for 6-10 hours to obtain black mulberry leaf enzymatic hydrolyzate, then inactivating the enzymatic hydrolyzate, sieving and setting aside;

[0025] 3) Secondary fermentation: preparing a fermentation medium with enzymolyzate, glucose and peptone in a mass ratio of 10:3:1, inoculating the fermentation medium with a plant lactobacillus solution of 5% of the volume of the fermentation medium, and fermenting at room temperature for 10-20 days to obtain a secondary fermentation liquid, filtering the obtained secondary fermentation liquid, concentrating the obtained supernatant to obtain a black mulberry leaf fermentation extract, and then freeze-drying it to prepare a powdered black mulberry leaf extract.

[0026] Furthermore, in the above-mentioned moisturizing and whitening cream, the composite extraction enzyme is composed of cellulase and polyphenol oxidase in a mass ratio of 1:2; the concentration of the lactobacillus liquid is 10 7 CFU / ml.

[0027] The last technical solution provided by the present invention is the above-mentioned moisturizing and whitening cream, which comprises the following steps in sequence:

[0028] (1) weighing each raw material according to the weight percentage described in the first technical solution for use;

[0029] (2) Add cetearyl alcohol, soy lecithin, poloxamer, dibutyl lauroyl glutamine, and oil to the oil phase pot in sequence, raise the temperature to 80-90° C. in a water bath under stirring, and keep warm for 15-20 minutes;

[0030] (3) Add carbomer, disodium cocoyl ethylenediamine PEG-15 disulfate, tetrasodium glutamate diacetate, polyol, and deionized water into a water phase pot, raise the temperature of the water bath to 80-90° C. under stirring, and keep warm for 15-20 minutes;

[0031] (4) Add the materials in the oil phase pot and the water phase pot into a homogenizer and homogenize at 8000-12000 rpm for 5-30 min at a temperature of 50-60° C. until a uniform emulsion is formed and the temperature drops below 40° C.;

[0032] (5) Stir the remaining components at a temperature of 25-40°C until dissolved and then add them to the system of step (4). Continue homogenizing for 10-15 minutes. After observing that the material is uniform, transfer it to a mixer and stir for 10-15 minutes. Cool it to room temperature and then discharge it.

[0033] Compared with the prior art, the technical solution provided by the present invention has the following technical advantages:

[0034] 1. The moisturizing and whitening cream provided by the present invention adopts the synergistic formulation of components such as oil, protease glucan, mannan, tocopherol, cetearyl alcohol, rice fermentation product, black mulberry leaf extract, purslane extract, etc., which can effectively promote the growth of new cells and the detachment of old keratin, inhibit the growth of melanin, and also inhibit the activity of tyrosinase, reduce the synthesized melanin or inhibit the self-oxidation of dopa, remove hydroxyl radicals and superoxide radicals, enhance the self-repair ability of the skin, and lighten the antioxidant properties of spots; at the same time, it replenishes the moisture required by the skin in time, and effectively reduces the amount of lipid peroxides in the human body, has a strong activating effect on luciferase, and is effective in preventing skin inflammation; it can change the composition of secreted sebum, help the skin absorb moisture, reduce the evaporation of epidermal moisture, repair the barrier function, have the effect of moisturizing and softening the skin, and at the same time enhance the skin permeability and promote the skin to absorb active ingredients.

[0035] 2. The oil provided by the present invention is compounded with tea seed oil, rose essential oil and tomato seed oil, and is rich in oleic acid, vitamins, tea polyphenols, squalene, terpenoids, lycopene, vitamin E and vitamin A. It is easily absorbed by the skin, promotes the decomposition of melanin, improves dry skin, restores skin elasticity, effectively promotes skin metabolism, and prevents skin wrinkles and roughness. It restores the skin's natural elasticity, makes it shiny and tender, thereby achieving the purpose of fading spots; it protects moisture and nourishes the skin, achieving the effect of locking water and moisturizing. In addition, it can effectively resist ultraviolet damage and has the effects of anti-ultraviolet and anti-radiation.

[0036] 3. The technical solution provided by the present invention also includes Sanguisorba officinalis root extract, Rhizoma Coptidis leaf extract, and Cabbage leaf extract, which further enhance free radical scavenging ability, can resist oxidative damage to the skin caused by harmful substances, can promote the synthesis of skin collagen and the renewal of skin cells, thereby promoting skin regeneration and repair; repair the skin, absorb and lock the moisture on the skin surface, form a protective layer, prevent the evaporation of moisture, and thus keep the skin hydrated and soft.

[0037] 4. The black mulberry leaf extract provided in the technical solution provided by the present invention is obtained by ultrafine grinding, soaking, and multiple fermentation extraction. After being formulated with other components of the present application, it is mild and non-irritating, inhibits or reduces melanin in multiple directions, accelerates skin metabolism, promotes melanin shedding, reduces ultraviolet radiation, replenishes the moisture needed by the skin, and can effectively improve microcirculation. DETAILED DESCRIPTION

[0038] The technical scheme of the present invention is further described in detail below in conjunction with specific embodiments, but the present invention is not limited to the following embodiments. It is worth noting that, unless otherwise specified, the various components used in the present invention are existing materials in the art and can be purchased through commercial channels or prepared by existing methods.

[0039] The rice fermentation product filtrate was purchased from Wuhan Haishan Technology Co., Ltd.;

[0040] Camellia sinensis leaf extract was purchased from Hubei Yuancheng Saichuang Technology Co., Ltd.;

[0041] The extract of golden bamboo leaves and plantain fruit extract were purchased from Fufeng Snot Biotechnology Co., Ltd.;

[0042] Purslane extract and Glycyrrhiza inflata root extract were purchased from Xi'an Tiankang Biotechnology Co., Ltd.

[0043] Sanguisorba officinalis root extract was purchased from Xi'an Anao Biotechnology Co., Ltd.;

[0044] Cabbage leaf extract was purchased from Baoji Fangsheng Biological Development Co., Ltd.

[0045] The black mulberry leaf extract was prepared by ourselves, and the preparation method thereof is as follows:

[0046] 1) ultrafinely grinding black mulberry leaves to 100 μm, adding 3 times the mass of water, soaking at room temperature for 24 hours, and centrifuging to obtain a black mulberry leaf soaking extract;

[0047] 2) primary fermentation: adding sodium dihydrogen phosphate-citric acid buffer solution to the black mulberry leaf soaking extract of step 1) to adjust the pH value to neutral, then adding 1.5wt% of the composite extraction enzyme to the black mulberry leaf soaking extract, fermenting and stirring at 35-40° C. for 8 hours to obtain a black mulberry leaf enzymatic hydrolyzate, then inactivating the enzymatic hydrolyzate, sieving and setting aside;

[0048] 3) Secondary fermentation: preparing a fermentation medium with enzymolyzate, glucose and peptone in a mass ratio of 10:3:1, inoculating the fermentation medium with a plant lactobacillus solution of 5% of the volume of the fermentation medium, and fermenting at room temperature for 15 days to obtain a secondary fermentation liquid, filtering the obtained secondary fermentation liquid, concentrating the obtained supernatant to obtain a black mulberry leaf fermentation extract, and then freeze-drying it to prepare a powdered black mulberry leaf extract.

[0049] Example 1

[0050] The present embodiment provides a moisturizing and whitening cream, comprising the following components in percentage by weight: 3% oil, 2% 1,3-propylene glycol, 6% glycerol, 0.5% protease dextran, 2% mannan, 5% tocopherol, 2% cetearyl alcohol, 2% rice fermentation product filtrate, 0.5% black mulberry leaf extract, 1% camellia leaf extract, 0.8% plantain fruit extract, 0.3% glycyrrhiza inflata root extract, 0.5% purslane extract, 0.4% disodium dicocoyl ethylenediamine PEG-15 disulfate, 1.25% soy lecithin, 0.5% poloxamer 124, 0.4% tetrasodium glutamate diacetate, 0.2% allantoin, 0.3% carbomer, 0.2% dibutyl lauroyl glutamine, 1% preservative, and an appropriate amount of water.

[0051] Example 2

[0052] The present embodiment provides a moisturizing and whitening cream, comprising the following components in percentage by weight: 10% oil, 2% 1,3-propylene glycol, 2% protease glucan, 0.2% mannan, 3% tocopherol, 5% cetearyl alcohol, 1% rice fermentation product filtrate, 0.7% black mulberry leaf extract, 2% camellia leaf extract, 1% plantain fruit extract, 0.2% licorice root extract, 0.8% purslane extract, 0.2% disodium dicocoyl ethylenediamine PEG-15 disulfate, 2.5% soy lecithin, 1.2% poloxamer 184, 0.5% tetrasodium glutamate diacetate, 0.3% allantoin, 0.1% carbomer, 0.8% dibutyl lauroyl glutamine, 0.8% preservative, and an appropriate amount of water.

[0053] Example 3

[0054] The present embodiment provides a moisturizing and whitening cream, comprising the following components in percentage by weight: 1% oil, 6% polyol, 1.2% protease glucan, 0.5% mannan, 7% tocopherol, 4% cetearyl alcohol, 4% rice fermentation product filtrate, 1.2% black mulberry leaf extract, 3% camellia leaf extract, 0.7% plantain fruit extract, 0.3% glycyrrhiza inflata root extract, 0.2% purslane extract, 0.4% disodium dicocoyl ethylenediamine PEG-15 disulfate, 1% soy lecithin, 0.8% poloxamer 188, 0.1% tetrasodium glutamate diacetate, 0.1% allantoin, 0.3% carbomer, 1% dibutyl lauroyl glutamine, 0.5% preservative, and an appropriate amount of water.

[0055] Example 4

[0056] The present embodiment provides a moisturizing and whitening cream, comprising the following components in percentage by weight: 5% oil, 2% 1,2-pentanediol, 3% glycerol, 1% protease glucan, 1% mannan, 5% tocopherol, 3% cetearyl alcohol, 3% rice fermentation product filtrate, 0.8% black mulberry leaf extract, 2% camellia leaf extract, 0.6% plantain fruit extract, 0.3% glycyrrhiza inflata root extract, 0.5% purslane extract, 0.5% disodium dicocoyl ethylenediamine PEG-15 disulfate, 1.5% soy lecithin, 0.9% poloxamer 188, 0.3% tetrasodium glutamate diacetate, 0.2% allantoin, 0.5% carbomer, 0.5% dibutyl lauroyl glutamine, 1% preservative, and an appropriate amount of water.

[0057] Example 5

[0058] The present embodiment provides a moisturizing and whitening cream, comprising the following components in percentage by weight: 5% oil, 2% 1,2-pentanediol, 3% glycerol, 1% protease glucan, 1% mannan, 5% tocopherol, 3% cetearyl alcohol, 3% rice fermentation product filtrate, 0.8% black mulberry leaf extract, 2% camellia leaf extract, 0.6% plantain fruit extract, 0.3% glycyrrhiza inflata root extract, 0.5% purslane extract, 0.5% disodium dicocoyl ethylenediamine PEG-15 disulfate, 1.5% soy lecithin, 0.9% poloxamer 188, 0.3% tetrasodium glutamate diacetate, 0.2% allantoin, 0.5% carbomer, 0.5% dibutyl lauroyl glutamine, 0.5% sanguisorba officinalis root extract, 1% preservative, and an appropriate amount of water.

[0059] Example 6

[0060] The present embodiment provides a moisturizing and whitening cream, comprising the following components in percentage by weight: 5% oil, 2% 1,2-pentanediol, 3% glycerol, 1% protease glucan, 1% mannan, 5% tocopherol, 3% cetearyl alcohol, 3% rice fermentation product filtrate, 0.8% black mulberry leaf extract, 2% camellia leaf extract, 0.6% plantain fruit extract, 0.3% glycyrrhiza inflata root extract, 0.5% purslane extract, 0.5% disodium dicocoyl ethylenediamine PEG-15 disulfate, 1.5% soy lecithin, 0.9% poloxamer 188, 0.3% tetrasodium glutamate diacetate, 0.2% allantoin, 0.5% carbomer, 0.5% dibutyl lauroyl glutamine, 1.0% sanguisorba officinalis root extract, 1% preservative, and an appropriate amount of water.

[0061] Example 7

[0062] The present embodiment provides a moisturizing and whitening cream, comprising the following components in percentage by weight: 5% oil, 2% 1,2-pentanediol, 3% glycerol, 1% protease glucan, 1% mannan, 5% tocopherol, 3% cetearyl alcohol, 3% rice fermentation product filtrate, 0.8% black mulberry leaf extract, 2% camellia leaf extract, 0.6% plantain fruit extract, 0.3% glycyrrhiza inflata root extract, 0.5% purslane extract, 0.5% disodium dicocoyl ethylenediamine PEG-15 disulfate, 1.5% soy lecithin, 0.9% poloxamer 188, 0.3% tetrasodium glutamate diacetate, 0.2% allantoin, 0.5% carbomer, 0.5% dibutyl lauroyl glutamine, 0.8% sanguisorba officinalis root extract, 1% preservative, and an appropriate amount of water.

[0063] Example 8

[0064] The present embodiment provides a moisturizing and whitening cream, comprising the following components in percentage by weight: 5% oil, 2% 1,2-pentanediol, 3% glycerol, 1% protease glucan, 1% mannan, 5% tocopherol, 3% cetearyl alcohol, 3% rice fermentation product filtrate, 0.8% black mulberry leaf extract, 2% camellia leaf extract, 0.6% plantain fruit extract, 0.3% glycyrrhiza inflata root extract, 0.5% purslane extract, 0.5% disodium dicocoyl ethylenediamine PEG-15 disulfate, 1.5% soy lecithin, 0.9% poloxamer 188, 0.3% tetrasodium glutamate diacetate, 0.2% allantoin, 0.5% carbomer, 0.5% dibutyl lauroyl glutamine, 0.8% sanguisorba officinalis root extract, 0.4% cabbage leaf extract, 0.05% petal leaf extract, 1% preservative, and an appropriate amount of water.

[0065] Example 9

[0066] The present embodiment provides a moisturizing and whitening cream, comprising the following components in percentage by weight: 5% oil, 2% 1,2-pentanediol, 3% glycerol, 1% protease glucan, 1% mannan, 5% tocopherol, 3% cetearyl alcohol, 3% rice fermentation product filtrate, 0.8% black mulberry leaf extract, 2% camellia leaf extract, 0.6% plantain fruit extract, 0.3% glycyrrhiza inflata root extract, 0.5% purslane extract, 0.5% disodium dicocoyl ethylenediamine PEG-15 disulfate, 1.5% soy lecithin, 0.9% poloxamer 188, 0.3% tetrasodium glutamate diacetate, 0.2% allantoin, 0.5% carbomer, 0.5% dibutyl lauroyl glutamine, 0.8% sanguisorba officinalis root extract, 0.4% cabbage leaf extract, 0.12% petal leaf extract, 1% preservative, and an appropriate amount of water.

[0067] Example 10

[0068] The present embodiment provides a moisturizing and whitening cream, comprising the following components in percentage by weight: 5% oil, 2% 1,2-pentanediol, 3% glycerol, 1% protease glucan, 1% mannan, 5% tocopherol, 3% cetearyl alcohol, 3% rice fermentation product filtrate, 0.8% black mulberry leaf extract, 2% camellia leaf extract, 0.6% plantain fruit extract, 0.3% glycyrrhiza inflata root extract, 0.5% purslane extract, 0.5% disodium dicocoyl ethylenediamine PEG-15 disulfate, 1.5% soy lecithin, 0.9% poloxamer 188, 0.3% tetrasodium glutamate diacetate, 0.2% allantoin, 0.5% carbomer, 0.5% dibutyl lauroyl glutamine, 0.8% sanguisorba officinalis root extract, 0.08% valeriana leaf extract, 0.4% cabbage leaf extract, 1% preservative, and an appropriate amount of water.

[0069] The oils and fats described in Examples 1 to 10 are prepared by compounding tea seed oil, rose essential oil and tomato seed oil in a mass ratio of 2:2:1.

[0070] The preservative consists of phenoxyethanol and ethylhexylglycerin in a mass ratio of 1:1.

[0071] The preparation method of the moisturizing and whitening cream in the above-mentioned embodiments 1-10 comprises the following steps:

[0072] 1) Weigh the raw materials according to the weight percentages described in Examples 1-10 for later use;

[0073] (2) adding cetearyl alcohol, soy lecithin, poloxamer, dibutyl lauroyl glutamine and oil to the oil phase pot in sequence, raising the temperature in a water bath to 80-90° C. (preferably 85° C.) under stirring, and keeping warm for 15-20 min (preferably 20 min);

[0074] (3) Add carbomer, disodium cocoyl ethylenediamine PEG-15 disulfate, tetrasodium glutamate diacetate, polyol, and deionized water into a water phase pot, raise the temperature of the water bath to 80-90° C. (preferably 85° C.) under stirring, and keep warm for 15-20 min; (preferably 20 min);

[0075] (4) Add the materials in the oil phase pot and the water phase pot into a homogenizer and homogenize at 50-60° C. (preferably 55° C.) and 8000-12000 rpm for 5-30 min (preferably 20 min) until a uniform emulsion is formed and the temperature drops below 40° C.;

[0076] (5) Stir the remaining components until dissolved at a temperature of 25-40°C (preferably 30°C) and add them to the system of step (4). Continue homogenizing for 10-15 minutes (preferably 15 minutes). After observing that the material is uniform, transfer it to a mixer and stir for 10-15 minutes (preferably 15 minutes). Cool it to room temperature and then discharge it.

[0077] In order to verify the synergistic effect of the technical solution provided by this application, some other cases in the research and development process of this application are given below as comparative examples.

[0078] Comparative Example 1

[0079] The present embodiment provides a moisturizing and whitening cream, comprising the following components in percentage by weight: 5% oil, 2% 1,2-pentanediol, 3% glycerol, 1% mannan, 6% tocopherol, 3% cetearyl alcohol, 3% rice fermentation product filtrate, 0.8% black mulberry leaf extract, 2% camellia leaf extract, 0.6% plantain fruit extract, 0.3% licorice root extract, 0.5% purslane extract, 0.5% disodium dicocoyl ethylenediamine PEG-15 disulfate, 1.5% soy lecithin, 0.9% poloxamer 188, 0.3% tetrasodium glutamate diacetate, 0.2% allantoin, 0.5% carbomer, 0.5% dibutyl lauroyl glutamine, 0.8% sanguisorba officinalis root extract, 0.08% valeriana leaf extract, 0.4% cabbage leaf extract, 1% preservative, and an appropriate amount of water.

[0080] Comparative Example 2

[0081] The present embodiment provides a moisturizing and whitening cream, comprising the following components in percentage by weight: 5% oil, 2% 1,2-pentanediol, 3% glycerol, 1% protease glucan, 1% mannan, 5% tocopherol, 3% cetearyl alcohol, 3% rice fermentation product filtrate, 0.8% black mulberry leaf extract, 2.3% camellia leaf extract, 0.6% plantain fruit extract, 0.5% purslane extract, 0.5% disodium dicocoyl ethylenediamine PEG-15 disulfate, 1.5% soy lecithin, 0.9% poloxamer 188, 0.3% tetrasodium glutamate diacetate, 0.2% allantoin, 0.5% carbomer, 0.5% dibutyl lauroyl glutamine, 0.8% sanguisorba officinalis root extract, 0.08% valeriana leaf extract, 0.4% cabbage leaf extract, 1% preservative, and an appropriate amount of water.

[0082] Comparative Example 3

[0083] The present embodiment provides a moisturizing and whitening cream, comprising the following components in percentage by weight: 5% oil, 2% 1,2-pentanediol, 3% glycerol, 1% protease glucan, 1% mannan, 5% tocopherol, 3% cetearyl alcohol, 4.1% rice fermentation product filtrate, 0.8% black mulberry leaf extract, 2% camellia leaf extract, 0.3% glycyrrhiza inflata root extract, 0.5% disodium dicocoyl ethylenediamine PEG-15 disulfate, 1.5% soy lecithin, 0.9% poloxamer 188, 0.3% tetrasodium glutamate diacetate, 0.2% allantoin, 0.5% carbomer, 0.5% dibutyl lauroyl glutamine, 0.8% sanguisorba officinalis root extract, 0.08% valeriana leaf extract, 0.4% cabbage leaf extract, 1% preservative, and an appropriate amount of water.

[0084] Comparative Example 4

[0085] The present embodiment provides a moisturizing and whitening cream, comprising the following components in percentage by weight: 5% oil, 2% 1,2-pentanediol, 3% glycerol, 1% protease glucan, 1% mannan, 5% tocopherol, 3% cetearyl alcohol, 3% rice fermentation product filtrate, 2.8% black mulberry leaf extract, 0.6% plantain fruit extract, 0.3% licorice inflata root extract, 0.5% purslane extract, 0.5% disodium dicocoyl ethylenediamine PEG-15 disulfate, 1.5% soy lecithin, 0.9% poloxamer 188, 0.3% tetrasodium glutamate diacetate, 0.2% allantoin, 0.5% carbomer, 0.5% dibutyl lauroyl glutamine, 0.8% sanguisorba officinalis root extract, 0.08% valeriana leaf extract, 0.4% cabbage leaf extract, 1% preservative, and an appropriate amount of water.

[0086] Comparative Example 5

[0087] The present embodiment provides a moisturizing and whitening cream, comprising the following components in percentage by weight: 5% rose essential oil, 2% 1,2-pentanediol, 3% glycerol, 1% protease glucan, 1% mannan, 5% tocopherol, 3% cetearyl alcohol, 3% rice fermentation product filtrate, 0.8% black mulberry leaf extract, 2% camellia leaf extract, 0.6% plantain fruit extract, 0.3% glycyrrhiza inflata root extract, 0.5% purslane extract, 0.5% disodium dicocoyl ethylenediamine PEG-15 disulfate, 1.5% soy lecithin, 0.9% poloxamer 188, 0.3% tetrasodium glutamate diacetate, 0.2% allantoin, 0.5% carbomer, 0.5% dibutyl lauroyl glutamine, 0.8% sanguisorba officinalis root extract, 0.08% valeriana leaf extract, 0.4% cabbage leaf extract, 1% preservative, and an appropriate amount of water.

[0088] The oils and fats described in Comparative Examples 1 to 5 are compounded with tea seed oil, rose essential oil and tomato seed oil in a mass ratio of 2:2:1.

[0089] Comparative Example 6

[0090] The present embodiment provides a moisturizing and whitening cream, comprising the following components in percentage by weight: 5% camellia oil, 2% 1,2-pentanediol, 3% glycerol, 1% protease glucan, 1% mannan, 5% tocopherol, 3% cetearyl alcohol, 3% rice fermentation product filtrate, 0.8% black mulberry leaf extract, 2% camellia leaf extract, 0.6% plantain fruit extract, 0.3% glycyrrhiza inflata root extract, 0.5% purslane extract, 0.5% disodium dicocoyl ethylenediamine PEG-15 disulfate, 1.5% soy lecithin, 0.9% poloxamer 188, 0.3% tetrasodium glutamate diacetate, 0.2% allantoin, 0.5% carbomer, 0.5% dibutyl lauroyl glutamine, 0.8% sanguisorba officinalis root extract, 0.08% valeriana leaf extract, 0.4% cabbage leaf extract, 1% preservative, and an appropriate amount of water.

[0091] Comparative Example 7

[0092] The present embodiment provides a moisturizing and whitening cream, comprising the following components in percentage by weight: 5% tomato seed oil, 2% 1,2-pentanediol, 3% glycerol, 1% protease glucan, 1% mannan, 5% tocopherol, 3% cetearyl alcohol, 3% rice fermentation product filtrate, 0.8% black mulberry leaf extract, 2% camellia leaf extract, 0.6% plantain fruit extract, 0.3% glycyrrhiza inflata root extract, 0.5% purslane extract, 0.5% disodium dicocoyl ethylenediamine PEG-15 disulfate, 1.5% soy lecithin, 0.9% poloxamer 188, 0.3% tetrasodium glutamate diacetate, 0.2% allantoin, 0.5% carbomer, 0.5% dibutyl lauroyl glutamine, 0.8% sanguisorba officinalis root extract, 0.08% valeriana leaf extract, 0.4% cabbage leaf extract, 1% preservative, and an appropriate amount of water.

[0093] The preparation method of the black mulberry leaf extract described in Comparative Examples 1-7 is the same as the preparation method of the black mulberry leaf extract described in the Examples.

[0094] Comparative Example 8

[0095] The present embodiment provides a moisturizing and whitening cream, comprising the following components in percentage by weight: 5% oil, 2% 1,2-pentanediol, 3% glycerol, 1% protease glucan, 1% mannan, 5% tocopherol, 3% cetearyl alcohol, 3% rice fermentation product filtrate, 0.8% black mulberry leaf extract, 2% camellia leaf extract, 0.6% plantain fruit extract, 0.3% glycyrrhiza inflata root extract, 0.5% purslane extract, 0.5% disodium dicocoyl ethylenediamine PEG-15 disulfate, 1.5% soy lecithin, 0.9% poloxamer 188, 0.3% tetrasodium glutamate diacetate, 0.2% allantoin, 0.5% carbomer, 0.5% dibutyl lauroyl glutamine, 0.8% sanguisorba officinalis root extract, 0.08% valeriana leaf extract, 0.4% cabbage leaf extract, 1% preservative, and an appropriate amount of water.

[0096] The black mulberry leaf extract in Comparative Example 8 was prepared by the following method:

[0097] Prepare an appropriate amount of fresh mulberry leaves, wash them with water and chop them into pieces; then add 10 times the amount of drinking water to soak for 1 hour, heat and boil to extract the distillate, and keep it slightly boiling for 2 hours; discharge the material, repeat the extraction once, and combine the two extracts to obtain the original solution; filter the original solution with a 100-mesh sieve; take the supernatant and concentrate it under reduced pressure at a temperature of 55°C to a relative density of 1.15 to obtain a concentrated solution, and freeze-dry it to obtain the finished product.

[0098] The types of preservatives described in Comparative Examples 1-8 are the same as those in the Examples, and unless otherwise specified, the sources of the raw materials are the same as those in the Examples.

[0099] The preparation methods of the moisturizing and whitening creams in the above comparative examples 1-8 are the same as the preparation methods of the moisturizing and whitening creams in the embodiments of the present invention, and the missing components are directly omitted.

[0100] In order to better illustrate the advantages of the present invention, the test experimental data of the present invention are given below:

[0101] 1. Whitening effect test

[0102] 1. Test products: products provided in Examples 1, 4, 5, 7, 8, 10, and products provided in Comparative Examples 1-8.

[0103] 2. Experimental population and methods: 70 healthy women aged 25-40 with dull skin and easy tanning were selected and randomly divided into 14 groups, with 5 people in each group. After cleaning the skin with warm water, the subjects in each group took 2 ml of the corresponding test product and applied it on the face once in the morning and evening every day for 4 weeks. During the experiment, the subjects were not allowed to use any other skin care products on the test area.

[0104] 3. Test instrument: Skin melanin and hemoglobin tester MX18

[0105] 4. Test method: Before use, use the skin melanin and hemoglobin tester MX18 to measure the highest point of each subject's right cheek on the 7th, 14th, 21st and 28th day, and take the average value of the three measurements.

[0106] 5. Test results:

[0107] Skin melanin content reduction rate = MI Tn -MI T0 / MI T0 ×100%

[0108] Where, MI Tn ——Tn value of the tested area

[0109] MIT0——Starting value of the tested area.

[0110] The results are shown in Table 1.

[0111] Table 1

[0112]

[0113]

[0114] Note: P<0.05.

[0115] It can be seen from Table 1 that the products provided in Examples 1, 4, 5, 7, 8, and 10 of the present application have significantly better inhibition rates on melanin and hemoglobin than the products provided in Examples 1-8.

[0116] 2. Inhibition of tyrosinase activity

[0117] 1. Test product: The products provided in Examples 1, 4, 5, 7, 8, and 10, and the products provided in Comparative Examples 1-8, are configured into 5 wt % test samples.

[0118] Positive control kojic acid, purity>98.5%: biochemical reagent BR;

[0119] Negative control: deionized water.

[0120] 2. Test method: T / GDCA 006-2021 Test method for inhibition of tyrosinase activity by cosmetic raw materials (in vitro method).

[0121] 3. The test result is the average value of three measurements; see Table 2 for specific results.

[0122] Inhibition rate of tyrosinase activity:

[0123]

[0124] Where: T-absorbance value of sample tube; T0-absorbance value of sample control tube; C-absorbance value of negative control tube; C0-absorbance value of blank control tube.

[0125] Table 2

[0126] Sample name Tyrosinase inhibition rate% SD P-value Example 1 73.25 8.01 ＜0.05 Example 4 77.92 7.22 ＜0.05 Example 5 78.93 6.17 ＜0.05 Example 7 81.2 6.24 ＜0.05 Example 8 82.81 7.50 ＜0.05 Example 10 83.72 4.61 ＜0.05 Comparative Example 1 60.21 5.16 ＜0.05 Comparative Example 2 63.78 6.25 ＜0.05 Comparative Example 3 54.56 5.63 ＜0.05 Comparative Example 4 49.61 6.22 ＜0.05 Comparative Example 5 45.86 5.85 ＜0.05 Comparative Example 6 39.09 8.78 ＜0.05 Comparative Example 7 43.92 9.78 ＜0.05 Comparative Example 8 52.07 6.61 ＜0.05 Positive control 84.23 4.51 ＜0.05 Negative control -0.86 2.14 /

[0127] It can be seen from the experimental results in Table 2 that the product provided in the embodiment has a stronger inhibitory effect on tyrosinase than the product provided in the comparative example, and can thus more effectively reduce the amount of melanin produced.

[0128] 3. Free radical scavenging rate

[0129] 1) Test samples: Test products: the products provided in Examples 1, 4, 5, 7, 8, 10, and the products provided in Comparative Examples 1-8, are respectively configured with pure water to form 2 wt % test samples.

[0130] 2) Test method: TSHRH006-2018 Cosmetics-Free Radical (DPPH) Scavenging Test Method

[0131] 3) Test results, take the average value of three measurements, as shown in Table 3.

[0132] DPPH free radical scavenging rate calculation formula:

[0133]

[0134] Where:

[0135] T-sample tube absorbance, that is, the absorbance of the solution after the sample reacts with DPPH;

[0136] T0-sample background absorbance;

[0137] The average value of the absorbance of the C-DPPH tube three times is the absorbance of the DPPH solution when no sample is added;

[0138] C0-solvent background absorbance.

[0139] Table 3

[0140] Serial number Sample name DPPH free radical scavenging rate (%) SD P-value 1 Example 1 75.92 6.10 ＜0.05 2 Example 4 80.18 6.04 ＜0.05 3 Example 5 81.10 4.91 ＜0.05 4 Example 7 83.17 4.86 ＜0.05 5 Example 8 84.64 5.94 ＜0.05 6 Example 10 86.38 7.65 <0.05 7 Comparative Example 1 64.03 6.75 ＜0.05 8 Comparative Example 2 58.17 4.97 ＜0.05 9 Comparative Example 3 49.76 4.48 ＜0.05 10 Comparative Example 4 45.24 4.94 ＜0.05 11 Comparative Example 5 41.82 4.65 ＜0.05 12 Comparative Example 6 35.65 6.93 ＜0.05 13 Comparative Example 7 40.06 7.78 ＜0.05 14 Comparative Example 8 47.49 5.26 ＜0.05 15 Positive control 86.17 3.37 ＜0.05 16 Negative control -0.93 3.57 /

[0141] It can be seen from the experimental results in Table 3 that the product provided by the embodiment has a stronger free radical scavenging rate than the product provided by the comparative example.

[0142] 4. Moisturizing effect test

[0143] 1. Test products: products provided in Examples 1, 4, 5, 7, 8, 10, and products provided in Comparative Examples 1-8.

[0144] 2. Experimental population and methods: 70 healthy women aged 25-40 with dull skin and easy tanning were selected and randomly divided into 14 groups, with 5 people in each group. After cleaning the skin with warm water, the subjects in each group took 2 ml of the corresponding test product and applied it on the face once in the morning and evening every day for 4 weeks. During the experiment, the subjects were not allowed to use any other skin care products on the test area.

[0145] 3. Test method: Before use, on the 7th, 14th, 21st and 28th day, the moisture content of the stratum corneum at the highest point of the right cheek of each subject was measured using the moisture tester Cormeometer CM825. The point-touch measurement was used and the average value was taken for 3 measurements. Before the test, the subject washed his face and rested quietly for 30 minutes.

[0146] 4. Test results:

[0147] Skin moisture content growth rate % = (MMV t -MMV0) / MMV0×100%;

[0148] MMV0: skin moisture content before use;

[0149] MMV t : Skin moisture content after t days of use.

[0150] The results are shown in Table 4.

[0151] Table 4

[0152] Grouping Growth rate on the 7th day (%) Growth rate on the 14th day (%) Growth rate on day 21 (%) Growth rate on the 28th day (%) Example 1 13.25 20.33 25.46 23.36 Example 4 14.79 22.13 24.75 22.65 Example 5 16.59 22.94 25.47 23.37 Example 7 18.36 25.46 26.06 23.96 Example 8 20.16 26.72 27.84 25.74 Example 10 20.85 28.53 28.45 26.35 Comparative Example 1 12.67 16.89 17.08 14.98 Comparative Example 2 13.95 14.61 15.06 12.96 Comparative Example 3 12.97 16.62 16.81 14.71 Comparative Example 4 13.79 14.78 17.20 15.10 Comparative Example 5 13.05 15.26 17.63 15.53 Comparative Example 6 12.96 17.38 19.48 17.38 Comparative Example 7 12.63 16.72 18.34 16.24 Comparative Example 8 12.02 16.15 18.05 15.95

[0153] Note: P<0.05.

[0154] It can be seen from the experimental results in Table 4 that the product provided in the embodiment has a better moisturizing effect than the product provided in the comparative example.

Claims

1. A moisturizing and whitening cream, characterized in that: The invention comprises the following components in weight percentage: 1-10% oil, 2-8% polyol, 0.5-2% protease dextran, 0.2-2% mannan, 3-7% tocopherol, 2-5% cetearyl alcohol, 1-5% rice fermentation product filtrate, 0.5-1.2% black mulberry leaf extract, 1-3% camellia leaf extract, 0.5-1% plantain fruit extract, 0.1-0.3% licorice root extract, 0.2-0.8% purslane extract, 0.2-1% disodium dicocoyl ethylenediamine PEG-15 disulfate, 1-2.5% soy lecithin, 0.5-1.2% poloxamer, 0.1-0.5% tetrasodium glutamate diacetate, 0.1-0.3% allantoin, 0.1-0.5% carbomer, 0.2-1% dibutyl lauroyl glutamine, 0.5-1% preservative, and appropriate amount of water; The oil is compounded by tea seed oil, rose essential oil and tomato seed oil in a mass ratio of 2:2:1; The black mulberry leaf extract is prepared by the following method: 1) ultrafinely grinding black mulberry leaves to 80-100 μm, adding 2-4 times the mass of water, soaking at room temperature for 12-36 hours, centrifuging and separating to obtain a black mulberry leaf soaking extract; 2) primary fermentation: adding sodium dihydrogen phosphate-citric acid buffer solution to the black mulberry leaf soaking extract of step 1) to adjust the pH value to neutral, then adding 1.0-2.0% composite extraction enzyme to the black mulberry leaf soaking extract, stirring and fermenting at 35-40° C. for 6-10 hours to obtain black mulberry leaf enzymatic hydrolyzate, then inactivating the enzymatic hydrolyzate, sieving and setting aside; 3) Secondary fermentation: preparing a fermentation medium with enzymolyzate, glucose and peptone in a mass ratio of 10:3:1, inoculating the fermentation medium with a plant lactobacillus solution of 5% of the volume of the fermentation medium, and fermenting at room temperature for 10-20 days to obtain a secondary fermentation liquid, filtering the obtained secondary fermentation liquid, concentrating the obtained supernatant to obtain a black mulberry leaf fermentation extract, and then freeze-drying it to prepare a powdered black mulberry leaf extract.

2. A moisturizing and whitening cream according to claim 1, characterized in that: Also includes Sanguisorba officinalis root extract 0.5-1.0%.

3. A moisturizing and whitening cream according to claim 2, characterized in that: It also includes 0.05-0.12% extract of golden bamboo leaves and 0.1-1.0% extract of cabbage leaves.

4. A moisturizing and whitening cream according to claim 1, characterized in that: The poloxamer is poloxamer 124, poloxamer 184 or poloxamer 188.

5. A moisturizing and whitening cream according to claim 1, characterized in that: The preservative is a complex of phenoxyethanol and ethylhexylglycerin.

6. A moisturizing and whitening cream according to claim 1, characterized in that: The polyol includes at least one of 1,3-propylene glycol, glycerol, 1,2-pentanediol, 1,2-hexanediol, and ethylhexylglycerol.

7. A moisturizing and whitening cream according to claim 1, characterized in that: The composite extraction enzyme is composed of cellulase and polyphenol oxidase in a mass ratio of 1:2; the concentration of the plant lactobacillus solution is 10 7 CFU / ml.

8. The method for preparing a moisturizing and whitening cream according to claim 3, characterized in that: The steps are as follows: (1) weighing each raw material according to the weight percentage for use; (2) Add cetearyl alcohol, soy lecithin, poloxamer, dibutyl lauroyl glutamine, and oil to the oil phase pot in sequence, raise the temperature to 80-90° C. in a water bath under stirring, and keep warm for 15-20 minutes; (3) Add carbomer, disodium cocoyl ethylenediamine PEG-15 disulfate, tetrasodium glutamate diacetate, polyol, and deionized water into a water phase pot, raise the temperature of the water bath to 80-90° C. under stirring, and keep warm for 15-20 minutes; (4) Add the materials in the oil phase pot and the water phase pot into a homogenizer and homogenize at 8000-12000 rpm for 5-30 min at a temperature of 50-60° C. until a uniform emulsion is formed and the temperature drops below 40° C.; (5) Stir the remaining components at a temperature of 25-40°C until dissolved and then add them to the system of step (4). Continue homogenizing for 10-15 minutes. After observing that the material is uniform, transfer it to a mixer and stir for 10-15 minutes. Cool it to room temperature and then discharge it.

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