A rapid detection device and method for bioluminescence intensity of traditional Chinese medicine decoction

Abstract

The invention discloses a device and method for rapid detection of bioluminescence intensity of traditional Chinese medicine decoction liquid, which relates to the technical field of traditional Chinese medicine quality detection, and comprises a main body mechanism, wherein the main body mechanism comprises a darkroom, a sample table is fixedly installed on the inner wall of the darkroom, a photomultiplier tube is fixedly installed on the top of the darkroom, a high-voltage power supply is fixedly installed on one side of the darkroom, the output end of the high-voltage power supply is electrically connected to the input end of the photomultiplier tube, a counting component is fixedly installed on the top of the darkroom, and the input end of the counting component is electrically connected to the output end of the photomultiplier tube. The device and method for rapid detection of bioluminescence intensity of traditional Chinese medicine decoction liquid utilize photomultiplier tubes, and photomultiplier tube detection can make up for some disadvantages of fluorescence sensor detection in terms of light source stability, interference signals, complexity of calibration and operation, and detection range and sensitivity, so as to rapidly and accurately evaluate the content of effective components in traditional Chinese medicine decoction liquid.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicine quality detection, and in particular to a device and method for rapid detection of bioluminescence intensity of traditional Chinese medicine decoction. Background Art

[0002] Chinese herbal medicine decoction, also known as Chinese herbal medicine decoction, is the liquid obtained by decocting Chinese herbal medicines in water. In Chinese medicine preparations, decoction is an important dosage form because it can extract the active ingredients in Chinese herbal medicines, making the drugs easier to be absorbed and utilized by the human body. With the advancement of science and technology, the quality inspection of Chinese herbal medicine decoction has become more and more important. By using bioluminescence technology, the quality of the decoction is evaluated by measuring the fluorescence intensity of the decoction, and the content of active ingredients in the Chinese herbal medicine decoction can be quickly and accurately detected.

[0003] The commonly used bioluminescence detection equipment for Chinese herbal medicine decoction is the Chinese herbal medicine bioluminescence analyzer, which is based on bioluminescence sensing technology. By detecting the luminescence intensity of bioluminescent substances in Chinese herbal medicine decoction under specific excitation light, the content of effective ingredients in the Chinese herbal medicine decoction can be quickly and accurately evaluated. Fluorescence sensing technology usually requires an external light source to excite the sample, which means that the stability of the light source will directly affect the accuracy of the test results. In order to obtain accurate quantitative results, fluorescence sensing technology needs to be calibrated and calibrated, which increases the complexity of the operation. To this end, we propose a rapid detection device and method for the bioluminescence intensity of Chinese herbal medicine decoction. Summary of the invention

[0004] The purpose of the present invention is to provide a device and method for rapid detection of bioluminescence intensity of traditional Chinese medicine decoction, so as to solve the problems raised in the above background technology.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a device and method for rapid detection of bioluminescence intensity of traditional Chinese medicine decoction, comprising a main body mechanism, the main body mechanism comprising a darkroom, a sample stage is fixedly installed on the inner wall of the darkroom, a photomultiplier tube is fixedly installed on the top of the darkroom, a high-voltage power supply is fixedly installed on one side of the darkroom, the output end of the high-voltage power supply is electrically connected to the input end of the photomultiplier tube, a counting component is fixedly installed on the top of the darkroom, the input end of the counting component is electrically connected to the output end of the photomultiplier tube, connecting holes are opened on both sides of the darkroom, a partition is rotatably connected to the inner wall of the connecting hole, and a pushing mechanism is arranged on one side of the darkroom;

[0006] The pushing mechanism includes a feeding unit, which is arranged at one side of the darkroom and is used to place the sample dish;

[0007] The pushing mechanism includes a pushing unit, which is arranged at one side of the darkroom and is used to push the sample dish to the sample stage;

[0008] A moving-out mechanism is arranged on the other side of the darkroom, and the moving-out mechanism is used to move the sample dish out of the sample stage.

[0009] Preferably, the feeding unit includes a connecting plate, which is fixedly installed on one side of the darkroom, a sliding groove is opened on the top of the connecting plate, a sliding block is slidably connected to the inner cavity of the sliding groove, a placing plate is fixedly installed on the top of the sliding block, a first motor is fixedly installed on the top of the connecting plate, a collecting wheel is fixedly installed on the output end of the first motor, a pull rope is fixedly installed on the surface of the collecting wheel, the surface of the pull rope is wound around the surface of the collecting wheel, one end of the pull rope is fixedly connected to the top of the sliding block, and a guide rod is rotatably connected to the inner wall of the sliding groove, and the top of the guide rod is in contact with the surface of the pull rope.

[0010] Preferably, the pushing unit includes a support plate, the support plate is fixedly mounted on the top of the connecting plate, a placement hole is opened on the inner side of the support plate, a first electric telescopic rod is fixedly mounted on the inner side of the support plate, and an electric clamp is fixedly mounted on one end of the first electric telescopic rod.

[0011] Preferably, the moving-out mechanism comprises a fixing plate, which is fixedly mounted on the other side of the darkroom, a second electric telescopic rod is fixedly mounted on the inner side of the fixing plate, a fixing frame is fixedly mounted on the telescopic end of the second electric telescopic rod, a rotating block is fixedly mounted on the bottom of the fixing frame, a buckle is rotatably connected to the inner wall of the rotating block, a rotating hole is provided on the inner side of the fixing plate, a second motor is fixedly mounted on the bottom of the fixing plate, a rotating wheel is fixedly mounted on the output end of the second motor, the surface of the rotating wheel is rotatably connected to the inner cavity of the rotating hole, a moving outlet is provided on one side of the fixing plate, and an inclined plate is fixedly mounted on the inner wall of the moving outlet.

[0012] Preferably, a guide groove is provided on the inner wall of the sliding groove, a guide block is fixedly mounted on the surface of the sliding block, and an inner cavity of the guide groove is slidably connected to the surface of the guide block.

[0013] Preferably, a limiting groove is provided on the inner side of the rotating block, a limiting rod is fixedly mounted on one side of the buckle, and the surface of the limiting rod is slidably connected to the inner cavity of the limiting groove.

[0014] Preferably, a friction pad is fixedly mounted on the surface of the rotating wheel, and the friction pad is made of rubber.

[0015] Preferably, a connecting block is fixedly installed on the bottom of the fixing frame, a spring is provided on the surface of the connecting block, one end of the spring is fixedly connected to the surface of the connecting block, and the other end of the spring is fixedly connected to the surface of the buckle.

[0016] Preferably, a guide plate is fixedly mounted on the top of the fixing plate, and the inner side of the guide plate is in contact with the surface of the sample stage.

[0017] Preferably, the method for using the device for rapid detection of bioluminescence intensity of traditional Chinese medicine decoction comprises the following steps:

[0018] S1: stack the sample dishes with Chinese medicine decoction on the top of the placement plate in sequence, start the first motor to drive the collecting wheel to reel in the pull rope, so that the sliding block moves in the inner cavity of the sliding groove, and the placement plate starts to move upwards through the sliding block, so that the sample dishes pass through the placement holes in sequence, and the sample dishes are pushed;

[0019] S2: Through the cooperation of the first electric telescopic rod and the electric clamp, the electric clamp clamps the sample dish, pushes the sample dish into the inner cavity of the darkroom through the first electric telescopic rod, and places the sample dish on the sample table, thereby completing the placement of the sample dish;

[0020] S3: After the sample dish is placed on the sample table, the darkroom can shield the surrounding light sources, and the shutter of the photomultiplier tube is opened at the same time by controlling the computer, and the photomultiplier tube is powered by a high-voltage power supply. The photomultiplier tube can detect the light source of the bioluminescent substance in the Chinese medicine decoction, and convert the detected light signal into an electrical signal, and transmit the converted electrical signal to the counting component for counting, and process the received signal, and transmit the collected information to the computer monitor for display, thereby completing the detection;

[0021] S4: After the detection is completed, the buckle passes through the top of the sample dish and contacts one side of the sample dish. The second electric telescopic rod is retracted to move the sample dish out of the inner cavity of the darkroom. The rotating wheel rotates to drive the sample dish to be moved out.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] 1. The present invention utilizes a photomultiplier tube, which can make up for some of the drawbacks of fluorescence sensing detection in terms of light source stability, interference signals, complexity of calibration and operation, and detection range and sensitivity, and can provide a rapid and accurate assessment of the content of active ingredients in Chinese herbal medicine decoctions.

[0024] 2. The present invention utilizes a pushing mechanism to realize automatic and accurate placement of sample dishes on the sample table, thereby reducing interference from human operation and improving detection accuracy and efficiency. The placed sample dishes are blocked by a connecting plate, so that the sample dishes can be kept stacked vertically. By moving the pull rope, the sample dishes are driven to enter the inner cavity of the support plate in turn, and by the cooperation of the first electric telescopic rod and the electric clamp, they are driven to enter the inner cavity of the darkroom and accurately placed on the sample table, thereby reducing interference from human operation and improving detection accuracy and efficiency.

[0025] 3. The present invention utilizes a removal mechanism to start the second electric telescopic rod to push the fixed frame forward. During the movement, the buckle first contacts the sample dish, and the buckle rotates and passes through the sample dish. The buckle can be smoothly reset to clamp one side of the sample dish. Then the second electric telescopic rod begins to retract, so that the sample dish is moved out of the inner cavity of the darkroom. By rotating the rotating wheel, the sample dish is automatically removed, and the sample dish slides out along the inclined plate, thereby completing the entire removal process, ensuring the stability and accuracy of the removal process. At the same time, the entire removal process does not require manual intervention, which greatly improves the detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a structural schematic diagram of the present invention;

[0027] Figure 2 It is a structural schematic diagram of the pushing unit of the present invention;

[0028] Figure 3 It is a structural schematic diagram of the feeding unit of the present invention;

[0029] Figure 4 It is a structural schematic diagram of the removal mechanism of the present invention;

[0030] Figure 5 It is a schematic diagram of the structure of the rotating block and the buckle of the present invention;

[0031] Figure 6 It is a schematic diagram of the structure of the connection hole and the partition plate of the present invention;

[0032] Figure 7 This is a structural exploded view of the rotating block and the buckle of the present invention;

[0033] Figure 8 The graph is a linear graph showing that the bioluminescence intensity of the Chinese medicine decoction of the present invention increases with the increase of the added medicine volume;

[0034] Fig. 9 The UPE strength change curve of the Panax notoginseng decoction according to the present invention as the storage time increases;

[0035] Fig.10The UPE strength change curve of the tangerine peel of the present invention as the storage time increases;

[0036] Fig.11 This is a curve diagram showing the total amount of ginsenosides Rb, Rg, and R in Panax notoginseng as the storage time increases;

[0037] Fig.12 The figure is a curve diagram showing the change of naringin content in the tangerine peel according to the present invention as the storage time increases.

[0038] In the figure: 1. main body; 11. darkroom; 12. sample stage; 13. photomultiplier tube; 14. high voltage power supply; 15. counting assembly; 16. connecting hole; 17. partition; 2. pushing mechanism; 21. feeding unit; 2101. connecting plate; 2102. sliding groove; 2103. sliding block; 2104. placing plate; 2105. first motor; 2106. collecting wheel; 2107. pull rope; 2108. guide rod; 2109. guide groove; 2110. guide block; 22. pushing mechanism Unit; 2201, support plate; 2202, placement hole; 2203, first electric telescopic rod; 2204, electric clamp; 3, removal mechanism; 31, fixed plate; 32, second electric telescopic rod; 33, fixed frame; 34, rotating block; 35, buckle; 36, rotating hole; 37, second motor; 38, rotating wheel; 39, removal outlet; 310, inclined plate; 311, limiting groove; 312, limiting rod; 313, friction pad; 314, connecting block; 315, spring; 316, guide plate. DETAILED DESCRIPTION

[0039] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] Embodiment 1

[0041] See also Figure 1-7The present invention provides a technical solution: a device and method for rapid detection of bioluminescence intensity of traditional Chinese medicine decoction liquid. The present invention makes corresponding improvements to the technical problems mentioned in the background technology, including a main body mechanism 1, the main body mechanism 1 includes a darkroom 11, a sample table 12 is fixedly installed on the inner wall of the darkroom 11, a photomultiplier tube 13 is fixedly installed on the top of the darkroom 11, a high-voltage power supply 14 is fixedly installed on one side of the darkroom 11, the output end of the high-voltage power supply 14 is electrically connected to the input end of the photomultiplier tube 13, a counting component 15 is fixedly installed on the top of the darkroom 11, the input end of the counting component 15 is electrically connected to the output end of the photomultiplier tube 13, connecting holes 16 are opened on both sides of the darkroom 11, a partition 17 is rotatably connected to the inner wall of the connecting hole 16, and a pushing mechanism 2 is arranged on one side of the darkroom 11;

[0042] The pushing mechanism 2 includes a feeding unit 21, which is disposed at one side of the darkroom 11 and is used to place the sample dishes;

[0043] The pushing mechanism 2 includes a pushing unit 22, which is disposed at one side of the darkroom 11 and is used to push the sample dish to the sample stage 12;

[0044] A removal mechanism 3 is provided on the other side of the darkroom 11, and the removal mechanism 3 is used to move the sample dish out of the sample stage 12;

[0045] The feeding unit 21 includes a connecting plate 2101, which is fixedly installed on one side of the darkroom 11. A sliding groove 2102 is provided on the top of the connecting plate 2101. A sliding block 2103 is slidably connected to the inner cavity of the sliding groove 2102. A placing plate 2104 is fixedly installed on the top of the sliding block 2103. A first motor 2105 is fixedly installed on the top of the connecting plate 2101. A collecting wheel 2106 is fixedly installed on the output end of the first motor 2105. A pull rope 2107 is fixedly installed on the surface of the collecting wheel 2106. The surface of the pull rope 2107 is wound around the surface of the collecting wheel. One end of the pull rope 2107 is fixedly connected to the top of the sliding block 2103. A guide rod 2108 is rotatably connected to the inner wall of the sliding groove 2102. The top of the guide rod 2108 contacts the surface of the pull rope 2107.

[0046] A guide groove 2109 is formed on the inner wall of the sliding groove 2102 , a guide block 2110 is fixedly mounted on the surface of the sliding block 2103 , and the inner cavity of the guide groove 2109 is slidably connected to the surface of the guide block 2110 .

[0047] The specific implementation of this embodiment is as follows: when in use, the sample dish is placed on the top of the placement plate 2104, and the moving position of the sample dish is shielded by the side plate of the connecting plate 2101. The side plate of the connecting plate 2101 covers half of the sample dish, so that the sample dishes can be kept stacked vertically together. The first motor 2105 is started by an external power supply, and the first motor 2105 drives the collecting wheel 2106 to rotate, so that the pull rope 2107 can be rolled up, and the guide rod 2108 is moved in the inner part of the sliding groove 2102. The rotation of the wall can change the moving angle of the pull rope 2107, which can drive the sliding block 2103 to move upward in the inner cavity of the sliding groove 2102. At the same time, through the sliding connection between the guide groove 2109 and the guide block 2110, the sliding block 2103 can be driven to keep moving vertically upward in the inner cavity of the sliding groove 2102, and can drive the sample dish above the placement plate 2104 to keep moving vertically to above the connecting plate 2101, so that multiple sample dishes can be loaded according to the placement order, thereby realizing automatic feeding of the sample dishes.

[0048] Embodiment 2

[0049] See also Figure 1-7 The present invention provides a technical solution: a device and method for rapid detection of bioluminescence intensity of traditional Chinese medicine decoction. The present invention makes corresponding improvements to the technical problems mentioned in the background technology. The pushing unit 22 includes a support plate 2201, which is fixedly installed on the top of the connecting plate 2101. A placement hole 2202 is provided on the inner side of the support plate 2201. A first electric telescopic rod 2203 is fixedly installed on the inner side of the support plate 2201, and an electric clamp 2204 is fixedly installed on one end of the first electric telescopic rod 2203.

[0050] The specific implementation of this embodiment is as follows: when the placement plate 2104 drives the sample dish to move upward, the uppermost sample dish passes through the placement hole 2202, and the first electric telescopic rod 2203 is started by an external power supply, and the electric clamping claw 2204 is pushed to approach the uppermost sample dish through the telescopic end of the first electric telescopic rod 2203, and the electric clamping claw 2204 clamps the sample dish. After clamping, the first electric telescopic rod 2203 is driven to move, pass through the connecting hole 16, and collide with the partition 17, so that the partition 17 is driven to rotate, so that the connecting hole 16 is opened, and the electric clamping claw 2204 carries the sample dish into the inner cavity of the darkroom 11, so that the sample dish can be placed at a slightly higher position directly above the sample stage 12. By loosening the driving clamping claw, the sample dish can be placed directly above the sample stage 12, and the first electric telescopic rod 2203 is moved out of the inner cavity of the darkroom 11. The partition 17 blocks the connecting hole 16 to ensure that the darkroom 11 The sealing performance of the sample dish is good. After the sample dish is placed on the sample table 12, the darkroom 11 can block the surrounding light sources. The shutter of the photomultiplier tube 13 is opened at the same time by computer control. The photomultiplier tube 13 is powered by the high-voltage power supply 14. The photomultiplier tube 13 can detect the light source of the bioluminescent substance in the Chinese medicine decoction, can detect weak light signals, and its amplification factor. The photomultiplier tube 13 does not rely on a specific excitation light source during the detection process, so it is not limited by the stability of the light source, and can provide more stable detection results. The detected light signal is converted into an electrical signal, and the converted electrical signal is transmitted to the counting component 15 for counting. The counting component 15 includes a photon counter and a counter card. The photon counter is used to identify the received electrical signal. The counter card processes the received signal and transmits the collected information to the computer display for display, thereby completing the detection of the bioluminescence intensity in the Chinese medicine decoction;

[0051] Configuration of photomultiplier tube 13: ET Enterprises, 9235QA (weak light sensitivity reaches 10-17W / cm2, background noise can reach 6-7 photons / second at 20℃)

[0052] Configuration of high voltage power supply 14: Sens-Tech, PM20SP;

[0053] Configuration of photon counter: HAMAMATSU, C9744;

[0054] Configuration of counter card: National Instruments, NIPCIe-6320.

[0055] Embodiment 3

[0056] See also Figure 1-7The present invention provides a technical solution: a device and method for rapid detection of bioluminescence intensity of Chinese herbal medicine decoction liquid. The present invention makes corresponding improvements to the technical problems mentioned in the background technology. The removal mechanism 3 includes a fixed plate 31, which is fixedly installed on the other side of the darkroom 11. A second electric telescopic rod 32 is fixedly installed on the inner side of the fixed plate 31. A fixed frame 33 is fixedly installed on the telescopic end of the second electric telescopic rod 32. A rotating block 34 is fixedly installed on the bottom of the fixed frame 33. A buckle 35 is rotatably connected to the inner wall of the rotating block 34. A rotating hole 36 is opened on the inner side of the fixed plate. A second motor 37 is fixedly installed on the bottom of the fixed plate 31. A rotating wheel 38 is fixedly installed on the output end of the second motor 37. The surface of the rotating wheel 38 is rotatably connected to the inner cavity of the rotating hole 36. A removal port 39 is opened on one side of the fixed plate 31. An inclined plate 310 is fixedly installed on the inner wall of the removal port 39.

[0057] A limiting groove 311 is formed on the inner side of the rotating block 34, and a limiting rod 312 is fixedly mounted on one side of the buckle 35, and the surface of the limiting rod 312 is slidably connected with the inner cavity of the limiting groove 311;

[0058] A friction pad 313 is fixedly mounted on the surface of the rotating wheel 38, and the friction pad 313 is made of rubber;

[0059] A connecting block 314 is fixedly installed at the bottom of the fixing frame 33, and a spring 315 is arranged on the surface of the connecting block 314, one end of the spring 315 is fixedly connected to the surface of the connecting block 314, and the other end of the spring 315 is fixedly connected to the surface of the buckle 35;

[0060] A guide plate 316 is fixedly mounted on the top of the fixing plate 31 , and the inner side of the guide plate 316 is in contact with the surface of the sample stage 12 .

[0061] The specific implementation of this embodiment is as follows: after the detection is completed, the second electric telescopic rod 32 is started by an external power supply, and the fixing frame 33 is driven by the second electric telescopic rod 32 to enter the connecting hole 16 on the other side, and enter the inner cavity of the darkroom 11. During the movement, the back of the buckle 35 will contact the surface of the sample dish in advance, and the sample dish is partially blocked by the sample stage 12, so that the buckle 35 can pass through the sample dish, so that the buckle 35 starts to rotate on the inner wall of the rotating block 34 and contacts with the top of the sample dish, and at the same time drives the spring 315 to stretch. After the buckle 35 passes through the sample dish, the spring 315 is supported by the connecting block 314, and the buckle 35 is rotated under the reaction force of the spring 315, and cooperates with the limiting rod 312 to slide on the inner wall of the limiting groove 311, so that the position of the buckle 35 is reset. The buckle 35 is made to contact one side of the sample dish, and the second electric telescopic rod 32 is started again to drive the fixing frame 33 to re-enter the inner side of the fixing plate 31. In this process, the two sides of the sample dish are shielded by the guide plate 316, so that the sample dish can be moved along the inner side of the fixing plate 31 and moved out of the inner cavity of the darkroom 11. When the sample dish moves to the outside of the darkroom 11, the second motor 37 is started by an external power supply, and the rotating wheel 38 is driven to rotate by the second motor 37. The friction force after the bottom of the sample dish contacts the rotating wheel 38 is increased by the friction pad 313, and the sample dish is driven to move out of the removal outlet 39 and to the inclined plate 310. The sample dish can be moved along the inclined plate 310 by its own gravity, so that the sample dish moves out of the inner side of the fixing plate 31, and the removal of the sample dish is completed.

[0062] Embodiment 4

[0063] (1) Preparation of Chinese herbal medicine decoction

[0064] Weigh 40 g of Chinese medicinal materials, put them in a casserole, add 800 mL of tap water and soak for 30 min, heat with an electric ceramic stove, adjust the fire to 2000, adjust the fire to 800 after boiling, decoct for 20 min, filter the medicinal liquid with 6 layers of gauze, repeat the above decoction process with the residue, combine and concentrate the medicinal liquids from the two decoctions, cool to room temperature, and finally obtain 200 mL of 0.2 g / mL Chinese medicinal decoction, and divide the Chinese medicinal decoction into 20 equal portions into sterile centrifuge tubes, and cool to room temperature away from light.

[0065] (2) Experimental conditions

[0066] Turn on the laboratory air conditioner 2 hours in advance, adjust the temperature to 20°C, and control the ambient humidity between 40% and 50%;

[0067] Device parameter settings:

[0068] Run the test software PC-device-DH, the test system starts self-checking, and after the system self-checking is completed, complete the setting of corresponding parameters in the operation interface:

[0069] Photomultiplier tube high voltage: 906V

[0070] Measurement mode: SE measurement

[0071] Measurement time interval: 1s

[0072] Measurement time: 10 minutes

[0073] The initial state of the filter: No filter.

[0074] (3) Experimental operation

[0075] A. After the power-on self-test process is completed and before the sample is tested, the system background noise is tested, recorded as background.

[0076] B. Turn off all light sources in the room where the test system is located, take 1 5mL portion of the Chinese medicine decoction and place it in a quartz dish, and place it on the sample table in the dark room. The whole process must be operated in a dark environment to prevent the Chinese medicine decoction from being affected by external light again and ensure that the test obtains the photon intensity of the Chinese medicine decoction itself;

[0077] C. Adjust the height of the sample stage so that its upper edge is close to the lower edge of the photomultiplier tube. The purpose is to shorten the distance between the sample and the photomultiplier tube probe as much as possible and collect the photons radiated by the sample itself to the greatest extent;

[0078] D. Confirm that the measurement parameters are correct, click "Measure" on the operating software to start counting;

[0079] E. After 10 minutes, the measurement is completed. Click the built-in data processing software to analyze the data and export the results.

[0080] The Chinese medicine liquid decocted on the same day was recorded as No. 1, and the remaining No. 2-8 were tested on the 3rd, 5th, 7th, 11th, 14th, 21st and 30th days after the Chinese medicine was decocted.

[0081] On the day when the bioluminescence intensity of the above-mentioned Chinese medicine decoction was measured, the content of its active ingredients was detected by HPLC, with the aim of conducting a correlation analysis between the luminescence intensity of the Chinese medicine decoction and the content of the active ingredients, using Pearson correlation analysis.

[0082] (4) Experimental results

[0083] The bioluminescence intensity of the Chinese medicine decoction solution increased linearly with the increase of the drug volume, indicating that the bioluminescence intensity was linearly related to the drug volume or drug concentration in the sample pool. Figure 8 As shown;

[0084] As the storage time increases, the UPE intensity of Panax notoginseng decoction and Citrus aurantium becomes lower. Therefore, as time goes by, the content of chemiluminescent groups in the Chinese medicine decoction decreases, and the number and type of organic molecules inside the Chinese medicine change. This change in molecular structure is consistent with the change in efficacy, such as Fig. 9 and Fig.10 As shown;

[0085] The changing trend of the effective ingredient contents of ginsenosides and naringin in the decoctions was the same as that of the decoctions of Panax notoginseng and Citrus aurantium, which further proved that the number and types of organic molecules in traditional Chinese medicines changed over time, and the efficacy of the medicines was destroyed. Fig.11 and Fig.12 shown.

[0086] (5) Pearson correlation analysis between UPE intensity and material content

[0087] Pearson correlation analysis was performed on the UPE intensity of Panax notoginseng decoction after different storage days and its corresponding total amount of ginsenosides Rb, Rg, and R, and the UPE intensity of Citrus aurantium wilfordii after different storage days and its corresponding naringin content. The results showed that with the extension of the storage time, the change in UPE intensity of the Chinese medicine decoction was significantly positively correlated with the content of its effective ingredients. The results are shown in the following table.

[0088] Pearson correlation analysis results of each indicator

[0089]

[0090]

[0091] Note: * indicates significant correlation at the 0.05 level, ** indicates significant correlation at the 0.001 level.

[0092] Through the correlation analysis of the UPE change trend of different Chinese herbal medicine decoctions and the change trend of the content of effective chemical components, it can be seen that UPE is significantly correlated with the effective drug components. UPE analysis can accurately identify that as time goes by, the effective drug components inside the Chinese herbal medicine decoction gradually change, and the concentration of the effective drug components also gradually decreases. This change in the effective components is significantly feasible through UPE detection.

[0093] Working principle: When in use, place the sample dish on the top of the placement plate 2104, and use the side plate of the connecting plate 2101 to block the moving position of the sample dish. The side plate of the connecting plate 2101 covers half of the sample dish, so that the sample dishes can be kept stacked vertically. Start the first motor 2105 through an external power supply, and drive the collecting wheel 2106 to rotate through the first motor 2105, so that the pull rope 2107 can be reeled in. The guide rod is rotated on the inner wall of the sliding groove 2102 to change the moving angle of the pull rope 2107, so that the sliding block 2103 can be driven to move upward in the inner cavity of the sliding groove 2102. At the same time, the guide groove 2109 is connected to the guide block 2110 through sliding, so that the sliding block 2103 is driven to move upward in the inner cavity of the sliding groove 2102. The inner cavity of the sliding groove 2102 keeps moving vertically upward, and can drive the sample dish above the placement plate 2104 to keep moving vertically to the top of the connecting plate 2101, so that multiple sample dishes can be loaded according to the placement order, and the automatic feeding of the sample dishes is realized. When the placement plate 2104 drives the sample dishes to move upward, the top sample dish passes through the placement hole 2202, and the first electric telescopic rod 2203 is started by an external power supply. The telescopic end of the first electric telescopic rod 2203 pushes the electric clamp 2204 to approach the top sample dish, and the electric clamp 2204 clamps the sample dish. After clamping, the first electric telescopic rod 2203 is driven to move, pass through the connecting hole 16, and engage with the partition 17. A collision occurs between them, driving the partition 17 to rotate, so that the connecting hole 16 is opened, and the electric clamp 2204 carries the sample dish into the inner cavity of the darkroom 11, so that the sample dish can be placed at a slightly higher position directly above the sample stage 12. By loosening the driving clamp, the sample dish can be placed directly above the sample stage 12, so that the first electric telescopic rod 2203 moves out of the inner cavity of the darkroom 11, and the partition 17 blocks the connecting hole 16 to ensure the sealing of the darkroom 11. After the sample dish is placed on the sample stage 12, the darkroom 11 can block the surrounding light sources, and the shutter of the photomultiplier tube 13 is opened at the same time by controlling the computer, and the photomultiplier tube 13 is powered by the high-voltage power supply 14, so that the photomultiplier tube 13 can detect the bioluminescent substances in the Chinese medicine decoction. The light source of quality is used, and the detected light signal is converted into an electrical signal, and the converted electrical signal is transmitted to the counting component 15 for counting, the received signal is processed, and the collected information is transmitted to the computer display for display, so as to complete the detection of the bioluminescence intensity in the Chinese medicine decoction. After the detection, the second electric telescopic rod 32 is started by an external power supply, and the fixing frame 33 is driven by the second electric telescopic rod 32 to enter the connecting hole 16 on the other side, and enter the inner cavity of the darkroom 11. During the movement, the back of the buckle 35 will contact the surface of the sample dish in advance, and the sample dish is partially blocked by the sample stage 12, so that the buckle 35 can pass through the sample dish, so that the buckle 35 starts to rotate on the inner wall of the rotating block 34 and contacts with the top of the sample dish.At the same time, the spring 315 is stretched. When the buckle 35 passes through the sample dish, the spring 315 is supported by the connecting block 314. Under the reaction force of the spring 315, the buckle 35 rotates and cooperates with the limiting rod 312 to slide on the inner wall of the limiting groove 311, so that the position of the buckle 35 is reset, so that the buckle 35 contacts one side of the sample dish, and the second electric telescopic rod 32 is started again to drive the fixing frame 33 to re-enter the inner side of the fixing plate 31. In this process, the two sides of the sample dish are blocked by the guide plate 316, so that the sample dish can be moved along The sample dish is moved along the inner side of the fixed plate 31, and the sample dish is moved out of the inner cavity of the darkroom 11. When the sample dish moves to the outside of the darkroom 11, the second motor 37 is started by an external power supply, and the second motor 37 drives the rotating wheel 38 to rotate. The friction pad 313 increases the friction between the bottom of the sample dish and the rotating wheel 38, and drives the sample dish to move out of the removal port 39 and move to the inclined plate 310. The sample dish can move along the inclined plate 310 by its own gravity, so that the sample dish moves out of the inner side of the fixed plate 31, and the removal of the sample dish is completed.

[0094] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0095] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A rapid detection device for bioluminescence intensity of Chinese herbal medicine decoction, comprising a main body (1), characterized in that: The main body mechanism (1) comprises a darkroom (11), a sample stage (12) is fixedly mounted on the inner wall of the darkroom (11), a photomultiplier tube (13) is fixedly mounted on the top of the darkroom (11), a high-voltage power supply (14) is fixedly mounted on one side of the darkroom (11), the output end of the high-voltage power supply (14) is electrically connected to the input end of the photomultiplier tube (13), a counting assembly (15) is fixedly mounted on the top of the darkroom (11), the input end of the counting assembly (15) is electrically connected to the output end of the photomultiplier tube (13), both sides of the darkroom (11) are provided with connection holes (16), the inner wall of the connection hole (16) is rotatably connected to a partition plate (17), and a pushing mechanism (2) is arranged on one side of the darkroom (11); The pushing mechanism (2) comprises a feeding unit (21), wherein the feeding unit (21) is arranged at one side of the darkroom (11), and the feeding unit (21) is used to place the sample dish; The pushing mechanism (2) comprises a pushing unit (22), wherein the pushing unit (22) is arranged at one side of the darkroom (11), and the pushing unit (22) is used to push the sample dish to the sample stage (12); A removal mechanism (3) is provided on the other side of the darkroom (11), and the removal mechanism (3) is used to remove the sample dish from the sample stage (12); The feeding unit (21) comprises a connecting plate (2101), the connecting plate (2101) is fixedly mounted on one side of the darkroom (11), a sliding groove (2102) is provided on the top of the connecting plate (2101), a sliding block (2103) is slidably connected to the inner cavity of the sliding groove (2102), a placing plate (2104) is fixedly mounted on the top of the sliding block (2103), a first motor (2105) is fixedly mounted on the top of the connecting plate (2101), and the first motor ( A collecting wheel (2106) is fixedly installed at the output end of the collecting wheel (2105), a pull rope (2107) is fixedly installed on the surface of the collecting wheel (2106), the surface of the pull rope (2107) is wound around the surface of the collecting wheel (2106), one end of the pull rope (2107) is fixedly connected to the top of the sliding block (2103), the inner wall of the sliding groove (2102) is rotatably connected to a guide rod (2108), and the top of the guide rod (2108) is in contact with the surface of the pull rope (2107); The pushing unit (22) comprises a support plate (2201), the support plate (2201) is fixedly mounted on the top of the connecting plate (2101), a placement hole (2202) is provided on the inner side of the support plate (2201), a first electric telescopic rod (2203) is fixedly mounted on the inner side of the support plate (2201), and an electric clamp (2204) is fixedly mounted on one end of the first electric telescopic rod (2203); The removal mechanism (3) comprises a fixing plate (31), the fixing plate (31) is fixedly mounted on the other side of the darkroom (11), a second electric telescopic rod (32) is fixedly mounted on the inner side of the fixing plate (31), a fixing frame (33) is fixedly mounted on the telescopic end of the second electric telescopic rod (32), a rotating block (34) is fixedly mounted on the bottom of the fixing frame (33), a buckle (35) is rotatably connected to the inner wall of the rotating block (34), a rotating hole (36) is provided on the inner side of the fixing plate (31), a second motor (37) is fixedly mounted on the bottom of the fixing plate (31), a rotating wheel (38) is fixedly mounted on the output end of the second motor (37), the surface of the rotating wheel (38) is rotatably connected to the inner cavity of the rotating hole (36), a removal outlet (39) is provided on one side of the fixing plate (31), an inclined plate (310) is fixedly mounted on the inner wall of the removal outlet (39); The inner wall of the sliding groove (2102) is provided with a guide groove (2109), the surface of the sliding block (2103) is fixedly mounted with a guide block (2110), and the inner cavity of the guide groove (2109) is slidably connected to the surface of the guide block (2110); A limiting groove (311) is provided on the inner side of the rotating block (34); a limiting rod (312) is fixedly mounted on one side of the buckle (35); and a surface of the limiting rod (312) is slidably connected to an inner cavity of the limiting groove (311); A friction pad (313) is fixedly mounted on the surface of the rotating wheel (38), and the friction pad (313) is made of rubber; A connecting block (314) is fixedly mounted on the bottom of the fixing frame (33); a spring (315) is arranged on the surface of the connecting block (314); one end of the spring (315) is fixedly connected to the surface of the connecting block (314); and the other end of the spring (315) is fixedly connected to the surface of the buckle (35); A guide plate (316) is fixedly mounted on the top of the fixed plate (31), and the inner side of the guide plate (316) is in contact with the surface of the sample stage (12).

2. The method for using the rapid detection device for bioluminescence intensity of Chinese herbal decoction according to claim 1, characterized in that: The following steps are involved: S1: stacking the sample dishes with Chinese medicine decoction on the top of the placement plate (2104) in sequence, starting the first motor (2105) to drive the collecting wheel (2106) to reel in the pull rope (2107), so that the sliding block (2103) moves in the inner cavity of the sliding groove (2102), and the placement plate (2104) starts to move upwards through the sliding block (2103), so that the sample dishes pass through the placement holes (2202) in sequence, and the sample dishes are pushed; S2: Through the cooperation between the first electric telescopic rod (2203) and the electric clamp (2204), the electric clamp (2204) clamps the sample dish, and the first electric telescopic rod (2203) pushes the sample dish into the inner cavity of the darkroom (11), and the sample dish is placed on the sample table (12), thereby completing the placement of the sample dish; S3: After the sample dish is placed on the sample table (12), the darkroom (11) can shield the surrounding light sources, and the shutter of the photomultiplier tube (13) is opened at the same time by controlling the computer, and the photomultiplier tube (13) is powered by the high-voltage power supply (14). The photomultiplier tube (13) can detect the light source of the bioluminescent substance in the Chinese medicine decoction, and convert the detected light signal into an electrical signal, and transmit the converted electrical signal to the counting component (15) for counting, and process the received signal, and transmit the collected information to the computer display for display, thereby completing the detection; S4: After the detection is completed, the buckle (35) passes through the top of the sample dish and contacts one side of the sample dish. The second electric telescopic rod (32) is retracted to move the sample dish out of the inner cavity of the darkroom (11). The rotating wheel (38) is rotated to drive the sample dish to be moved out.

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