A kind of preparation method of flurellana solution

Abstract

The invention relates to the technical field of insecticide preparation, and discloses a method for preparing a flurana solution, comprising the following steps: S1, pipetting an organic solvent into a clean glass three-necked bottle, then weighing a cosolvent and a stabilizer into the glass three-necked bottle in sequence, heating in an oil bath at 50°C, stirring and dissolving by magnetic force; S2, weighing a flurana raw material drug into the glass three-necked bottle, heating in an oil bath at 50°C, stirring and dissolving by magnetic force; S3, pipetting an organic solvent into a clean glass single-necked bottle, then weighing an antioxidant and a flavor corrector into the glass single-necked bottle in sequence, and dissolving by ultrasonic force. Flurana is well dissolved with the aid of an organic solvent cosolvent, and good solution stability is formed with a stabilizer, an antioxidant and an acidity regulator, thereby increasing the shelf life of the product; a suitable flavor corrector can promote the acceptance of animals and reduce the rejection of medication by medication animals; the invention has simple and safe ingredients, and is widely used.

Description

Technical Field

[0001] The invention relates to the technical field of insecticide preparation, and more specifically discloses a method for preparing a flurellan solution. Background Art

[0002] The new veterinary drug Fluralaner is a new type of isoxazoline broad-spectrum insecticide that interferes with the parasite's γ-aminobutyric acid (GABA)-gated chloride ion channels, causing overexcitement of the nervous system and death. Fluralaner has good insecticidal activity against pests such as Acarina, Siphonaptera, and Licea. It is mainly used to treat internal and external parasites of cats, dogs and other animals, as well as Dermanyssus gallinae and northern feather mites infections in chicks, breeders and laying hens. It is a highly effective insecticide. Compared with traditional insecticides, isoxazoline insecticides have fewer side effects on cats, dogs and chickens. The marketed varieties of Fluralaner include Fluralaner chewable tablets and Fluralaner topical solution, etc., with huge market prospects.

[0003] Patent application number: 202111631718.7, invention name: A flurella insecticide and its preparation method and application. The flurella insecticide provided by the present invention can ensure that flurella is not destroyed by water containing hypochlorous acid. Its composition is 2.5g flurella, 25g Tween-80, 25ml dimethylacetamide, 10g soybean oil, and anhydrous ethanol is made up to 100ml. This inventive preparation contains vegetable oil and is easily emulsified when diluted with water.

[0004] Patent application number: 202311565322.6, invention name: A fluororana insecticide and deodorant spray and its preparation method and application, the present invention can realize fluororana to make a deodorant spray, which is composed of 1g fluororana, 0.03g antioxidant, 7.5g transdermal agent and 7.5g deodorant, and the organic solvent is filled to 100ml. However, the spray is uneven and the absorption rate is low due to the blocking of animal hair, which affects the efficacy.

[0005] Patent application number: 202110943959.9, invention name: A kind of flurellana chewable tablet for pets and its preparation method. The present invention can realize the production of flurellana chewable tablets with stable properties and wide clinical application. Its composition is 50% flurellana, 20% fish meal, 10% sucrose, 10% corn starch, 3% sodium lauryl sulfate, 0.3% magnesium stearate, 5% soybean oil, and 6.7% polyethylene glycol, but it cannot meet the palatability of all animals, the ingredients are complex, the cost is high, and it can also cause vomiting, loss of appetite, gastrointestinal bloating and other adverse reactions.

[0006] The dosage form of the currently approved flurellan products is mainly chewable tablets, which have a series of problems such as high price and inconvenient feeding. Summary of the invention

[0007] The invention mainly provides a method for preparing a flurellan solution, which can solve the problems of high price and inconvenience in feeding of existing flurellan products.

[0008] To solve the above technical problems, according to one aspect of the present invention, more specifically, a flurella solution comprises the following components: flurella, a cosolvent, an acidity regulator, a stabilizer, an antioxidant, a flavor corrector and an organic solvent;

[0009] The cosolvent includes one or more of isooctyl alcohol, urethane, and 3-oxidinecarboxamide;

[0010] The acidity regulator includes one or more of citric acid, malic acid, and fumaric acid;

[0011] The stabilizer is β-cyclodextrin;

[0012] The antioxidant includes one or more of 2,3,5,6-tetrahydroxy-2-hexene-4-lactone, 2,6-di-tert-butyl-p-cresol, and cysteine;

[0013] The flavor corrective is 2-ethyl-3-hydroxy-4H-pyrone.

[0014] Furthermore, the volume of the flurellana solution is 1L, wherein: flurellana is 5.0-30.0g / L, the cosolvent is 1.0-5.0g / L, the acidity regulator is 0.5-2.0g / L, the stabilizer is 1.0-2.0g / L, the antioxidant is 2.0-5.0g / L, the flavor corrector is 1.0-5.0g / L, and the organic solvent is made up to 1L.

[0015] Furthermore, the volume of the flurellana solution is 100 ml, wherein: flurellana is specifically 1.0 g, the cosolvent is specifically 0.3 g, the acidity regulator is specifically 0.1 g, the stabilizer is specifically 0.1 g, the antioxidant is specifically 0.3 g, the flavor corrector is specifically 0.3 g, and the organic solvent is made up to 100 ml.

[0016] Furthermore, the organic solvent includes at least one of ethanol, hexadecanol, N,N-dimethylformamide and triacetin.

[0017] According to another aspect of the present invention, there is provided a method for preparing a flurellan solution, comprising the following steps:

[0018] S1. Pipette the organic solvent into a clean three-necked glass bottle, then weigh the co-solvent and stabilizer into the three-necked glass bottle in turn, heat in a 50°C oil bath and stir with magnetic stirring to dissolve;

[0019] S2, weigh the flurellanine API and add it into a three-necked glass bottle, heat it in an oil bath at 50°C and stir it with magnetic stirring to dissolve;

[0020] S3, transfer the organic solvent to a clean glass single-mouth bottle, then weigh the antioxidant and flavor corrective agent into the glass single-mouth bottle in sequence, and dissolve them by ultrasonication;

[0021] S4, adding the solution obtained in step S3 dropwise to the solution obtained in step S2, and stirring rapidly to mix evenly;

[0022] S5. Add the acidity regulator into a three-necked glass bottle, heat in a 50°C oil bath and stir with a magnetic stirrer to dissolve, and adjust the acidity of the system;

[0023] S6, cooling to room temperature, then transferring the organic solvent to a fixed volume, and dissolving by ultrasonication to obtain the flurellan solution.

[0024] The beneficial effects of the preparation method of a flurella solution of the present invention are as follows: flurella is well dissolved with the assistance of an organic solvent cosolvent, and good solution stability is formed with a stabilizer, an antioxidant and an acidity regulator, thereby increasing the shelf life of the product; a suitable flavor corrective can promote and improve the acceptance of animals and reduce the drug rejection of the animals taking the drug; the ingredients of the present invention are simple and safe, and are promoted to be widely used. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] The present invention is further described in detail below with reference to the accompanying drawings and specific implementation methods.

[0026] Figure 1 This is the liquid phase comparison spectrum of the long-term experiment in Example 1;

[0027] Figure 2 This is the liquid phase comparison spectrum of the long-term experiment in Example 2;

[0028] Figure 3 This is the liquid phase comparison spectrum of the long-term experiment in Example 3;

[0029] Figure 4 This is the liquid phase comparison spectrum of the long-term experiment in Example 4;

[0030] Figure 5 This is the liquid phase comparison spectrum of the long-term experiment in Example 5;

[0031] Figure 6 This is the liquid phase comparison spectrum of the long-term experiment in Example 6;

[0032] Figure 7 This is the liquid phase comparison spectrum of the long-term experiment in Example 7;

[0033] Figure 8 This is the liquid phase comparison spectrum of the long-term experiment in Example 8.

[0034] Fig. 9This is the liquid phase comparison spectrum of the high temperature illumination experiment in Example 1;

[0035] Fig.10 This is the liquid phase comparison spectrum of the high temperature illumination experiment in Example 2;

[0036] Fig.11 This is the liquid phase comparison spectrum of the high temperature illumination experiment in Example 3;

[0037] Fig.12 This is the liquid phase comparison spectrum of the high temperature illumination experiment in Example 4;

[0038] Fig.13 This is the liquid phase comparison spectrum of the high temperature illumination experiment in Example 5;

[0039] Fig.14 This is the liquid phase comparison spectrum of the high temperature illumination experiment in Example 6;

[0040] Fig.15 This is the liquid phase comparison spectrum of the high temperature illumination experiment in Example 7;

[0041] Fig.16 This is the liquid phase comparison spectrum of the high temperature illumination experiment in Example 8. DETAILED DESCRIPTION

[0042] The present invention will be described in detail below with reference to the accompanying drawings and in combination with embodiments. It should be noted that the embodiments and features in the embodiments of the present application can be combined with each other without conflict.

[0043] The flurella solution comprises flurella, a cosolvent, an acidity regulator, a stabilizer, an antioxidant, a flavor corrector and an organic solvent. The cosolvent comprises one or more of isooctyl alcohol, urethane and 3-oxazine carboxamide. The acidity regulator comprises one or more of citric acid, malic acid and fumaric acid. The stabilizer is β-cyclodextrin. The antioxidant comprises one or more of 2,3,5,6-tetrahydroxy-2-hexene-4-lactone, 2,6-di-tert-butyl-p-cresol and cysteine. The flavor corrector is 2-ethyl-3-hydroxy-4H-pyrone.

[0044] Example 1

[0045] A flurellane solution is prepared from 1.0 g of flurellane, 0.3 g of urethane, 0.1 g of citric acid, 0.1 g of beta-cyclodextrin, 0.3 g of 2,3,5,6-tetrahydroxy-2-hexene-4-lactone, 0.1 g of 2-ethyl-3-hydroxy-4H-pyrone, and the mixture is made up to 100 ml with a ratio of ethanol to n-butyl acetate of 2:1 (V / V).

[0046] (1) Pipette 10 ml of n-butyl acetate into a clean three-necked glass flask, weigh 0.3 g of urethane and 0.1 g of β-cyclodextrin, add them into the three-necked glass flask, heat in an oil bath at 50°C with magnetic stirring to dissolve;

[0047] (2) Weigh 1.0 g of flurellanine API and add it to (1), heat in a 50°C oil bath with magnetic stirring to dissolve;

[0048] (3) Pipette 20 ml of ethanol into a clean glass bottle, weigh 0.3 g of 2,3,5,6-tetrahydroxy-2-hexene-4-lactone and 0.1 g of 2-ethyl-3-hydroxy-4H-pyrone, and dissolve them by ultrasonication;

[0049] (4) Add the solution obtained in (3) dropwise to the solution obtained in (2), and stir rapidly under heating in a 50°C oil bath to mix evenly;

[0050] (5) Weigh 0.1 g of citric acid and add it to the solution obtained in (4), heat it in an oil bath at 50°C with magnetic stirring to dissolve it, and adjust the acidity of the system;

[0051] (6) Cooling to room temperature, and then using ethanol: n-butyl acetate = 2:1 (V / V) system to make the volume to 100 ml, and ultrasonically dissolving to obtain the flurellan solution.

[0052] Example 2

[0053] A flurellane solution is prepared from 1.0 g of flurellane, 0.3 g of urethane, 0.1 g of citric acid, 0.1 g of beta-cyclodextrin, 0.3 g of 2,3,5,6-tetrahydroxy-2-hexene-4-lactone, 0.2 g of 2-ethyl-3-hydroxy-4H-pyrone, and the mixture is made up to 100 ml with a ratio of ethanol to n-butyl acetate of 2:1 (V / V).

[0054] (1) Pipette 10 ml of n-butyl acetate into a clean three-necked glass flask, weigh 0.3 g of urethane and 0.1 g of β-cyclodextrin, add them into the three-necked glass flask, heat in an oil bath at 50°C with magnetic stirring to dissolve;

[0055] (2) Weigh 1.0 g of flurellanine API and add it to (1), heat in a 50°C oil bath with magnetic stirring to dissolve;

[0056] (3) Pipette 20 ml of ethanol into a clean glass bottle, weigh 0.3 g of 2,3,5,6-tetrahydroxy-2-hexene-4-lactone and 0.2 g of 2-ethyl-3-hydroxy-4H-pyrone, and dissolve them by ultrasonication;

[0057] (4) adding the solution obtained in (3) dropwise to the solution obtained in (2), and stirring rapidly under heating in a 50°C oil bath to mix evenly;

[0058] (5) Weigh 0.1 g of citric acid and add it to the solution obtained in (4), heat it in an oil bath at 50°C with magnetic stirring to dissolve it, and adjust the acidity of the system;

[0059] (6) Cooling to room temperature, and then using ethanol: n-butyl acetate = 2:1 (V / V) system to make the volume to 100 ml, and ultrasonically dissolving to obtain the flurellan solution.

[0060] Example 3

[0061] A flurellane solution comprises 1.0 g of flurellane, 0.3 g of urethane, 0.1 g of citric acid, 0.1 g of beta-cyclodextrin, 0.3 g of 2,3,5,6-tetrahydroxy-2-hexene-4-lactone, 0.3 g of 2-ethyl-3-hydroxy-4H-pyrone, and the mixture is made up to 100 ml with a ratio of ethanol to n-butyl acetate of 2:1 (V / V).

[0062] (1) Pipette 10 ml of n-butyl acetate into a clean three-necked glass flask, weigh 0.3 g of urethane and 0.1 g of β-cyclodextrin, add them into the three-necked glass flask, heat in an oil bath at 50°C with magnetic stirring to dissolve;

[0063] (2) Weigh 1.0 g of flurellanine API and add it to (1), heat in a 50°C oil bath with magnetic stirring to dissolve;

[0064] (3) Pipette 20 ml of ethanol into a clean glass bottle, weigh 0.3 g of 2,3,5,6-tetrahydroxy-2-hexene-4-lactone and 0.3 g of 2-ethyl-3-hydroxy-4H-pyrone, and dissolve them by ultrasonication;

[0065] (4) adding the solution obtained in (3) dropwise to the solution obtained in (2), and stirring rapidly under heating in a 50°C oil bath to mix evenly;

[0066] (5) Weigh 0.1 g of citric acid and add it to the solution obtained in (4), heat it in an oil bath at 50°C with magnetic stirring to dissolve it, and adjust the acidity of the system;

[0067] (6) Cooling to room temperature, and then using ethanol: n-butyl acetate = 2:1 (V / V) system to make the volume to 100 ml, and ultrasonically dissolving to obtain the flurellan solution.

[0068] Example 4

[0069] A flurellane solution comprises 1.0 g of flurellane, 0.3 g of urethane, 0.1 g of citric acid, 0.1 g of beta-cyclodextrin, 0.3 g of 2,3,5,6-tetrahydroxy-2-hexene-4-lactone, 0.4 g of 2-ethyl-3-hydroxy-4H-pyrone, and the mixture is made up to 100 ml with ethanol: n-butyl acetate = 2:1 (V / V).

[0070] (1) Pipette 10 ml of n-butyl acetate into a clean three-necked glass flask, weigh 0.3 g of urethane and 0.1 g of β-cyclodextrin, add them into the three-necked glass flask, heat in an oil bath at 50°C with magnetic stirring to dissolve;

[0071] (2) Weigh 1.0 g of flurellanine API and add it to (1), heat in a 50°C oil bath with magnetic stirring to dissolve;

[0072] (3) Pipette 20 ml of ethanol into a clean glass bottle, weigh 0.3 g of 2,3,5,6-tetrahydroxy-2-hexene-4-lactone and 0.4 g of 2-ethyl-3-hydroxy-4H-pyrone, and dissolve them by ultrasonication;

[0073] (4) adding the solution obtained in (3) dropwise to the solution obtained in (2), and stirring rapidly under heating in a 50°C oil bath to mix evenly;

[0074] (5) Weigh 0.1 g of citric acid and add it to the solution obtained in (4), heat it in an oil bath at 50°C with magnetic stirring to dissolve it, and adjust the acidity of the system;

[0075] (6) Cooling to room temperature, and then using ethanol: n-butyl acetate = 2:1 (V / V) system to make the volume to 100 ml, and ultrasonically dissolving to obtain the flurellan solution.

[0076] Example 5

[0077] A flurellane solution is prepared from 1.0 g of flurellane, 0.3 g of urethane, 0.1 g of citric acid, 0.1 g of beta-cyclodextrin, 0.3 g of 2,3,5,6-tetrahydroxy-2-hexene-4-lactone, 0.5 g of 2-ethyl-3-hydroxy-4H-pyrone, and the mixture is made up to 100 ml with a ratio of ethanol to n-butyl acetate of 2:1 (V / V).

[0078] (1) Pipette 10 ml of n-butyl acetate into a clean three-necked glass flask, weigh 0.3 g of urethane and 0.1 g of β-cyclodextrin, add them into the three-necked glass flask, heat in an oil bath at 50°C with magnetic stirring to dissolve;

[0079] (2) Weigh 1.0 g of flurellanine API and add it to (1), heat in a 50°C oil bath with magnetic stirring to dissolve;

[0080] (3) Pipette 20 ml of ethanol into a clean glass bottle, weigh 0.3 g of 2,3,5,6-tetrahydroxy-2-hexene-4-lactone and 0.5 g of 2-ethyl-3-hydroxy-4H-pyrone, and dissolve them by ultrasonication;

[0081] (4) adding the solution obtained in (3) dropwise to the solution obtained in (2), and stirring rapidly under heating in a 50°C oil bath to mix evenly;

[0082] (5) Weigh 0.1 g of citric acid and add it to the solution obtained in (4), heat it in an oil bath at 50°C with magnetic stirring to dissolve it, and adjust the acidity of the system;

[0083] (6) Cooling to room temperature, and then using ethanol: n-butyl acetate = 2:1 (V / V) system to make the volume to 100 ml, and ultrasonically dissolving to obtain the flurellan solution.

[0084] Example 6

[0085] A flurellane solution is prepared from 0.5 g of flurellane, 0.1 g of urethane, 0.05 g of citric acid, 0.1 g of beta-cyclodextrin, 0.2 g of 2,3,5,6-tetrahydroxy-2-hexene-4-lactone, 0.1 g of 2-ethyl-3-hydroxy-4H-pyrone, and the mixture is made up to 100 ml with a ratio of ethanol to n-butyl acetate of 2:1 (V / V).

[0086] (1) Pipette 10 ml of n-butyl acetate into a clean three-necked glass flask, weigh 0.1 g of urethane and 0.1 g of β-cyclodextrin, add them into the three-necked glass flask, heat in an oil bath at 50°C with magnetic stirring to dissolve;

[0087] (2) Weigh 0.5 g of flurellanine API and add it to (1), heat in a 50°C oil bath and stir with a magnetic stirrer to dissolve;

[0088] (3) Pipette 20 ml of ethanol into a clean glass bottle, weigh 0.2 g of 2,3,5,6-tetrahydroxy-2-hexene-4-lactone and 0.1 g of 2-ethyl-3-hydroxy-4H-pyrone, and dissolve them by ultrasonication;

[0089] (4) adding the solution obtained in (3) dropwise to the solution obtained in (2), and stirring rapidly under heating in a 50°C oil bath to mix evenly;

[0090] (5) Weigh 0.05 g of citric acid and add it to the solution obtained in (4), heat it in an oil bath at 50°C with magnetic stirring to dissolve it, and adjust the acidity of the system;

[0091] (6) Cooling to room temperature, and then using ethanol: n-butyl acetate = 2:1 (V / V) system to make the volume to 100 ml, and ultrasonically dissolving to obtain the flurellan solution.

[0092] Example 7

[0093] A flurellane solution is composed of 2.0 g of flurellane, 0.25 g of urethane, 0.15 g of citric acid, 0.15 g of beta-cyclodextrin, 0.25 g of 2,3,5,6-tetrahydroxy-2-hexene-4-lactone, 0.25 g of 2-ethyl-3-hydroxy-4H-pyrone, and ethanol: n-butyl acetate = 2:1 (V / V) to make up to 100 ml.

[0094] (1) Pipette 10 ml of n-butyl acetate into a clean three-necked glass flask, weigh 0.25 g of urethane and 0.15 g of β-cyclodextrin, add them into the three-necked glass flask, heat in an oil bath at 50°C with magnetic stirring to dissolve;

[0095] (2) Weigh 2.0 g of flurellanine API and add it to (1), heat in a 50°C oil bath with magnetic stirring to dissolve;

[0096] (3) Pipette 20 ml of ethanol into a clean glass bottle, weigh 0.25 g of 2,3,5,6-tetrahydroxy-2-hexene-4-lactone and 0.25 g of 2-ethyl-3-hydroxy-4H-pyrone, and dissolve them by ultrasonication;

[0097] (4) adding the solution obtained in (3) dropwise to the solution obtained in (2), and stirring rapidly under heating in a 50°C oil bath to mix evenly;

[0098] (5) Weigh 0.15 g of citric acid and add it to the solution obtained in (4), heat it in an oil bath at 50°C with magnetic stirring to dissolve it, and adjust the acidity of the system;

[0099] (6) Cooling to room temperature, and then using ethanol: n-butyl acetate = 2:1 (V / V) system to make the volume to 100 ml, and ultrasonically dissolving to obtain the flurellan solution.

[0100] Example 8

[0101] A flurellane solution is prepared from 3.0 g of flurellane, 0.3 g of urethane, 0.2 g of citric acid, 0.2 g of beta-cyclodextrin, 0.5 g of 2,3,5,6-tetrahydroxy-2-hexene-4-lactone, 0.5 g of 2-ethyl-3-hydroxy-4H-pyrone, and the mixture is made up to 100 ml with a ratio of ethanol to n-butyl acetate of 2:1 (V / V).

[0102] (1) Pipette 10 ml of n-butyl acetate into a clean three-necked glass flask, weigh 0.5 g of urethane and 0.2 g of β-cyclodextrin, add them into the three-necked glass flask, heat in an oil bath at 50°C with magnetic stirring to dissolve;

[0103] (2) Weigh 3.0 g of flurellanine API and add it to (1), heat in a 50°C oil bath and stir with a magnetic stirrer to dissolve;

[0104] (3) Pipette 20 ml of ethanol into a clean glass bottle, weigh 0.5 g of 2,3,5,6-tetrahydroxy-2-hexene-4-lactone and 0.5 g of 2-ethyl-3-hydroxy-4H-pyrone, and dissolve them by ultrasonication;

[0105] (4) adding the solution obtained in (3) dropwise to the solution obtained in (2), and stirring rapidly under heating in a 50°C oil bath to mix evenly;

[0106] (5) Weigh 0.2 g of citric acid and add it to the solution obtained in (4), heat it in an oil bath at 50°C with magnetic stirring to dissolve it, and adjust the acidity of the system;

[0107] (6) Cooling to room temperature, and then using ethanol: n-butyl acetate = 2:1 (V / V) system to make the volume to 100 ml, and ultrasonically dissolving to obtain the flurellan solution.

[0108] Stability test

[0109] 1. Long-term test

[0110] The solutions prepared in Examples 1-8 of the present invention were placed at room temperature for 12 months, and samples were taken to determine the main content and impurities, and observe the properties.

[0111]

[0112] 2. High temperature light test

[0113] The solutions prepared in Examples 1-8 of the present invention were placed under ultraviolet irradiation at 50° C. for 30 days, and samples were taken on the same day and 30 days to detect relevant indicators.

[0114]

[0115]

[0116] 3. Drug acceptance evaluation

[0117] At a laying hen breeding base, 32 naturally infected cases (4 in each example) were selected for drug acceptance evaluation. The drug acceptance evaluation of the present invention was based on the addition of flurella solution to the feed and the absence of flurella solution as a control. The difference in the feeding of each laying hen on the feed was checked in turn, and the phenomenon of not eating and anorexia was observed. The drug acceptance of each group was judged according to the following principles:

[0118] Good acceptance: After adding the drug to chicken feed, the food intake of the test laying hens was not affected, or the food intake was reduced to less than 20% of the normal food intake.

[0119] Poor acceptance: After adding the drug to chicken feed, the food intake of the test laying hens was significantly affected, or the food intake was reduced by more than 20% of the normal food intake.

[0120]

[0121]

[0122] 4. Efficacy evaluation

[0123] At a laying hen breeding base, 48 naturally infected cases (6 in each example) were selected for drug efficacy evaluation. The drug efficacy evaluation of the present invention uses the change index of the number of external parasitic mites and eggs in the skin and the clinical symptoms as the judgment criteria. The number of external parasitic mites and eggs of each laying hen was checked in turn, and the reduction rate of parasitic mites and eggs was calculated. The drug efficacy of each group was judged according to the following principles:

[0124] Effective: After the medication, the average reduction rate of the number of parasitic mites and eggs on the skin surface was >90%, there was no inflammation or rash in the affected area, and the test animals had no feather damage.

[0125] Ineffective: After the medication, the average reduction rate of the number of parasitic mites and eggs on the skin surface was <90%, the affected area still had inflammation, rashes, etc., and the test animals had feather damage.

[0126]

[0127]

[0128] It can be seen from the above embodiments 1-8 that the effect of embodiment 3 is the most obvious, and it is the most effective in treating infections of Dermatophytes gallinae and Northern Feather Mites on the surface of laying hens and in assisting the treatment of allergic dermatitis caused by mites. The product is more stable and has the least impurities.

[0129] Of course, the above description is not a limitation of the present invention, and the present invention is not limited to the above examples. Changes, modifications, additions or substitutions made by ordinary technicians in this technical field within the essential scope of the present invention also fall within the protection scope of the present invention.

Claims

1. A flurella solution, characterized in that: The invention comprises the following components: flurella, a cosolvent, an acidity regulator, a stabilizer, an antioxidant, a flavor corrector and an organic solvent; The cosolvent is urethane; The acidity regulator is citric acid; The stabilizer is β-cyclodextrin; The antioxidant is 2,3,5,6-tetrahydroxy-2-hexene-4-lactone; The flavor corrective is 2-ethyl-3-hydroxy-4H-pyrone.

2. A fluororana solution according to claim 1, characterized in that: The volume of the flurellana solution is 1L, wherein: flurellana is 5.0-30.0g / L, the cosolvent is 1.0-5.0g / L, the acidity regulator is 0.5-2.0g / L, the stabilizer is 1.0-2.0g / L, the antioxidant is 2.0-5.0g / L, the flavor corrector is 1.0-5.0g / L, and the organic solvent is made up to 1L.

3. A fluororana solution according to claim 1, characterized in that: The organic solvent includes at least one of ethanol, hexadecanol, N,N-dimethylformamide and triacetin.

4. A fluororana solution according to claim 2, characterized in that: The volume of the flurellana solution is 100 ml, wherein: flurellana is 1.0 g, the cosolvent is 0.3 g, the acidity regulator is 0.1 g, the stabilizer is 0.1 g, the antioxidant is 0.3 g, the flavor corrector is 0.3 g, and the organic solvent is used to make up to 100 ml.

5. The method for preparing a fluororana solution according to any one of claims 1 to 4, characterized in that: The following steps are involved: S1. Pipette the organic solvent into a clean three-necked glass flask, then weigh the co-solvent and stabilizer into the three-necked glass flask in turn, and heat and magnetically stir to dissolve; S2, weighing the flurellan API, adding it into a three-necked glass bottle, heating and magnetic stirring to dissolve; S3, transfer the organic solvent to a clean glass single-mouth bottle, then weigh the antioxidant and flavor corrective agent into the glass single-mouth bottle in sequence, and dissolve them by ultrasonication; S4, adding the solution obtained in step S3 dropwise to the solution obtained in step S2, and stirring rapidly to mix evenly; S5. Add the acidity regulator into the glass three-necked flask, heat and magnetically stir to dissolve, and adjust the acidity of the system; S6, cooling to room temperature, then transferring the organic solvent to a fixed volume, and dissolving by ultrasonication to obtain the flurellan solution.

6. The method for preparing a fluororana solution according to claim 5, characterized in that: In the steps S1, S2 and S3, the heating and magnetic stirring are all carried out in a 50°C oil bath.

7. A fluororana solution according to any one of claims 1 to 4, characterized in that: The solution can be used to treat and control Dermanyscus gallinae and Northern feather mite infestations in chicks, breeders and laying hens.

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