Method and device for determining sample suction compensation parameters, and sample suction equipment

Abstract

The present application provides a method and apparatus for determining a sample aspiration compensation parameter, and a sample aspiration device, relating to the field of medical technology. The method of one embodiment includes: obtaining a preset gas volume and a pressure curve of the sample aspiration and exhalation process of the sample aspiration device; based on the pressure curve, obtaining an initial pressure value before sample aspiration, a pressure value at the end of sample aspiration, and a pressure value at the end of exhalation; according to the relationship between gas volume and pressure, obtaining a gas volume at the end of sample aspiration and a gas volume at the end of exhalation based on the preset gas volume, the initial pressure value before sample aspiration, the pressure value at the end of sample aspiration, and the pressure value at the end of exhalation; and obtaining a sample aspiration compensation parameter based on the gas volume at the end of sample aspiration and the gas volume at the end of exhalation. The sample aspiration compensation parameter obtained by the solution of this embodiment has high accuracy, and performing sample aspiration compensation based on the sample aspiration compensation parameter can improve the accuracy of the sample aspiration amount of the sample aspiration device.

Description

Technical Field

[0001] The present application relates to the field of medical technology, and in particular to a method for determining a sample aspiration compensation parameter, an apparatus for determining a sample aspiration compensation parameter, a sample aspiration device, a computer-readable storage medium, and a computer program product. Background Art

[0002] The operation of in vitro diagnostic equipment often involves the aspiration and injection process of a sample suction device. For example, the sample suction device is equipped with a sample suction head (such as a liquid suction needle) through which the liquid sample is aspirated. The accuracy of the liquid volume aspirated by the liquid suction needle directly affects the accuracy of the relevant test results of the in vitro diagnostic device. Considering the influence of factors such as air pressure in different altitudes, when the liquid suction device is located at different altitudes, the sample aspiration amount is usually compensated based on the altitude. However, the sampling process of existing sample suction devices still has the problem of low accuracy of the liquid volume aspirated. Summary of the Invention

[0003] Based on this, it is necessary to provide a method for determining sampling compensation parameters, a device for determining sampling compensation parameters, a sampling device, a computer-readable storage medium and a computer program product that can improve the accuracy of the sampling amount of the sampling device in order to address the above technical problems.

[0004] In a first aspect, the present application provides a method for determining a sample aspiration compensation parameter, wherein the method comprises:

[0005] Obtain the preset gas volume and the pressure curve of the sampling device during the sampling process;

[0006] Based on the pressure curve, an initial pressure value before sample aspiration, a pressure value at the end of sample aspiration, and a pressure value at the end of sample discharge are obtained;

[0007] According to the relationship between gas volume and pressure, based on the initial pressure value before sample aspiration, the pressure value at the end of sample aspiration, and the pressure value at the end of sample exhalation, the gas volume at the end of sample aspiration and the gas volume at the end of sample exhalation are obtained;

[0008] A sample aspiration compensation parameter is obtained based on the sample aspiration end gas volume and the sample exhalation end gas volume.

[0009] The method for determining the aspiration compensation parameter according to the embodiment of the present application described above can obtain the initial pressure value before aspiration, the pressure value at the end of aspiration, and the pressure value at the end of exhalation during the actual aspiration and exhalation process of the aspiration device based on the pressure curve during the aspiration and exhalation process of the aspiration device. Based on this, the gas volume at the end of aspiration and the gas volume at the end of exhalation can be obtained based on the relationship between gas volume and pressure, and the aspiration compensation parameter can be obtained accordingly. The aspiration compensation parameter obtained in this manner is determined based on the actual aspiration and exhalation process of the aspiration device. The gas volume at the end of aspiration and the gas volume at the end of exhalation determined thereby can reflect the liquid volume at the end of aspiration and the liquid volume after the exhalation. Therefore, based on the gas volume at the end of aspiration and the gas volume at the end of exhalation, the changes in the liquid volume, the liquid volume at the end of aspiration, and the liquid volume after the exhalation process of the aspiration device can be accurately reflected. The aspiration compensation parameter determined thereby can accurately reflect the changes in the liquid volume of the aspiration device during the aspiration and exhalation process. The obtained aspiration compensation parameter has high accuracy. Performing aspiration compensation based on the aspiration compensation parameter can improve the accuracy of the aspiration volume of the aspiration device.

[0010] In some embodiments, the obtaining of the gas volume at the end of sample aspiration and the gas volume at the end of sample exhalation based on the relationship between gas volume and pressure and the preset gas volume, the initial pressure value before sample aspiration, the pressure value at the end of sample aspiration, and the pressure value at the end of sample exhalation includes:

[0011] Substituting the preset gas volume, the initial pressure value before sample aspiration, the pressure value at the end of sample aspiration, and the pressure value at the end of sample discharge into the van der Waals equation respectively to obtain a van der Waals equation group;

[0012] The van der Waals equations are solved to obtain the gas volume at the end of the sample aspiration and the gas volume at the end of the sample exhalation.

[0013] Based on this embodiment, after obtaining the initial pressure value before sample aspiration, the pressure value at the end of sample aspiration, and the pressure value at the end of sample exhalation from the pressure curve, since the van der Waals equation takes into account the size of the gas molecules themselves and the interaction force between molecules on the basis of the gas state equation, it better reflects and describes the macroscopic physics of the gas. Therefore, the gas volume at the end of sample aspiration and the gas volume at the end of sample exhalation determined based on this are more accurate. Determining the sample aspiration compensation parameters on this basis can also improve the accuracy of the obtained sample aspiration compensation parameters, and can also further improve the accuracy of the sample aspiration amount of the sample aspiration device.

[0014] In some embodiments, obtaining the sample aspiration compensation parameter based on the sample aspiration end gas volume and the sample exhalation end gas volume includes:

[0015] Calculating the volume difference between the gas volume at the end of the sample aspiration and the gas volume at the end of the sample exhalation;

[0016] The sample aspiration compensation parameter is determined based on the volume difference.

[0017] Based on this embodiment, by calculating the volume difference between the gas volume at the end of sample aspiration and the gas volume at the end of sample exhalation, and determining the sample aspiration compensation parameter based on the volume difference, the accuracy of the obtained sample aspiration compensation parameter is improved. Performing sample aspiration compensation based on the sample aspiration compensation parameter can improve the accuracy of the sample aspiration amount of the sample aspiration device.

[0018] In some embodiments, determining the sample aspiration compensation parameter based on the volume difference includes:

[0019] The volume difference is used as the sample aspiration compensation parameter.

[0020] Based on this embodiment, by using the volume difference between the gas volume at the end of sample aspiration and the gas volume at the end of sample exhalation as the sample aspiration compensation parameter, it is simple and convenient, and can improve the efficiency of the process of determining the sample aspiration compensation parameter.

[0021] In some embodiments, determining the sample aspiration compensation parameter based on the volume difference includes:

[0022] determining a correction coefficient based on the gas volume at the end of the sample aspiration and the gas volume at the end of the sample exhalation;

[0023] The volume difference is corrected based on the correction coefficient to obtain the sample aspiration compensation parameter.

[0024] Based on this embodiment, a correction coefficient is determined by the volume of gas at the end of sample aspiration and the volume of gas at the end of sample exhalation, and the volume difference is corrected using the correction coefficient to obtain a final sample aspiration compensation parameter. The obtained sample aspiration compensation parameter can reduce the influence of the error between the gas volume at the end of sample aspiration and the gas volume at the end of sample exhalation, further improve the accuracy of the obtained sample aspiration compensation parameter, and sample aspiration compensation is performed on this basis, which can also further improve the accuracy of sample aspiration.

[0025] In some embodiments, determining the correction coefficient based on the gas volume at the end of the sample aspiration and the gas volume at the end of the sample exhalation includes:

[0026] The correction coefficient is determined based on the ratio of the sum of the volume difference and the preset gas volume to the basic gas volume, where the basic gas volume is determined based on the preset gas volume and the preset sample addition amount during the suction and exhalation process.

[0027] Based on this embodiment, when determining the correction coefficient, it is determined based on the preset gas mention and the preset sample addition amount, so that it can be determined based on the preset sample addition amount of the aspiration and exhalation process in combination with the obtained volume difference, so that the obtained correction coefficient is determined in combination with the actual aspiration and exhalation process, so that the obtained correction coefficient is higher. Determining the aspiration compensation parameter based on the correction coefficient can further improve the accuracy of the obtained aspiration compensation parameter. Performing aspiration compensation on this basis can also further improve the accuracy of aspiration.

[0028] In some embodiments, obtaining a pressure curve of the sample aspiration process of the sample aspiration device includes:

[0029] Acquiring multiple initial pressure curves of the sample suction device during multiple sample suction and exhalation processes;

[0030] Averaging is performed on the multiple initial pressure curves to obtain the pressure curve.

[0031] Based on this embodiment, when obtaining the pressure curve of the suction and exhalation process of the sampling device, after the sampling device performs multiple suction and exhalation processes, homogenization processing is performed based on the initial pressure curves of multiple suction and exhalation processes to obtain the final pressure curve for analysis, thereby reducing the influence of individual differences in the initial pressure curve of only sucking and exhaling once. The determination of the suction compensation parameter based on the pressure curve after the average processing of multiple initial pressure curves can also make the finally obtained suction compensation parameter more accurate. Suction compensation is performed on this basis, which can further improve the accuracy of suction.

[0032] In a second aspect, the present application further provides a device for determining a sample aspiration compensation parameter, wherein the device comprises:

[0033] An information acquisition module is used to obtain the preset gas volume and the pressure curve of the sampling device during the sampling process;

[0034] A pressure value acquisition module, configured to obtain an initial pressure value before sample aspiration, a pressure value at the end of sample aspiration, and a pressure value at the end of sample discharge based on the pressure curve;

[0035] a volume parameter determination module, configured to obtain the gas volume at the end of sample aspiration and the gas volume at the end of sample exhalation according to the relationship between gas volume and pressure, based on the preset gas volume, the initial pressure value before sample aspiration, the pressure value at the end of sample aspiration, and the pressure value at the end of sample exhalation;

[0036] The compensation parameter determination module is configured to obtain a sample aspiration compensation parameter based on the sample aspiration end gas volume and the sample exhalation end gas volume.

[0037] In the third aspect, the present application also provides a sample suction device, which includes a sample suction head, a pressure sensor arranged on the liquid suction pipeline of the sample suction head, and a processor connected to the pressure sensor, wherein the pressure sensor collects and outputs the pressure of the sample suction device during the sample suction and exhalation process, and the processor obtains a pressure curve based on the pressure output by the pressure sensor, and determines the sample suction compensation parameters of the sample suction device based on the method in any of the embodiments described above.

[0038] In some embodiments, the processor is further configured to obtain a preset sample aspiration volume, determine a target sample aspiration volume based on the preset sample aspiration volume and the sample aspiration compensation parameter, and control the sample aspiration head to aspirate based on the target sample aspiration volume.

[0039] In a fourth aspect, the present application further provides a computer-readable storage medium having a computer program stored thereon, which, when executed by a processor, implements the steps of the method in any of the above embodiments.

[0040] In a fifth aspect, the present application further provides a computer program product, which includes a computer program that, when executed by a processor, implements the steps of the method in any of the above embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 Schematic diagram of an application scenario of a method for determining a sample aspiration compensation parameter in an embodiment;

[0042] Figure 2 1 is a flow chart of a method for determining a sample aspiration compensation parameter in one embodiment;

[0043] Figure 3 A schematic diagram of a process for obtaining a pressure curve in one embodiment;

[0044] Figure 4 Schematic diagram of a process for obtaining gas volume in one embodiment;

[0045] Figure 5 A schematic diagram of a process for obtaining sample aspiration compensation parameters in one embodiment;

[0046] Figure 6 FIG1 is a flow chart of determining the sample aspiration compensation parameter based on the volume difference in one embodiment;

[0047] Figure 7 is a schematic diagram of a pressure curve in a specific example;

[0048] Figure 8 Schematic diagram of the liquid aspiration and expulsion state of the sample aspirating needle in a specific example;

[0049] Figure 9 FIG. 4 is a structural block diagram of a device for determining sample aspiration compensation parameters in an embodiment. DETAILED DESCRIPTION

[0050] In order to make the purpose, technical solutions and advantages of this application more clear, the following further describes this application in detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain this application and are not intended to limit this application.

[0051] The following embodiments of the technical solution of the present application will be described in detail with reference to the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of the present application and are therefore only examples and are not intended to limit the scope of protection of the present application.

[0052] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this application belongs; the terms used herein are only for the purpose of describing specific embodiments and are not intended to limit this application; the terms "including" and "having" and any variations thereof in the specification and claims of this application and the above-mentioned figure descriptions are intended to cover non-exclusive inclusions.

[0053] In the description of the embodiments of this application, the technical terms "first" and "second" are used only to distinguish different objects and should not be understood to indicate or imply relative importance or implicitly specify the quantity, specific order, or primary and secondary relationship of the indicated technical features. In the description of the embodiments of this application, the meaning of "plurality" is more than two, unless otherwise clearly and specifically defined.

[0054] References herein to "embodiments" mean that a particular feature, structure, or characteristic described in connection with the embodiments may be included in at least one embodiment of the present application. The appearance of this phrase in various places in the specification does not necessarily refer to the same embodiment, nor does it constitute an independent or alternative embodiment that is mutually exclusive of other embodiments. It is understood, both explicitly and implicitly, by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0055] In the description of the embodiments of the present application, the term "multiple" refers to more than two (including two). Similarly, "multiple groups" refers to more than two groups (including two groups), and "multiple pieces" refers to more than two pieces (including two pieces).

[0056] In the field of medical technology, when aspirating liquid using a sampling device, when the sampling device is located at different altitudes, due to the influence of altitude, it is necessary to perform sampling compensation processing on the sample volume to reduce the impact of altitude on the accuracy of sampling. Sampling compensation processing for the sample volume refers to compensating the sample volume for a required preset sample volume when aspirating the sample using the sampling device to obtain an adjusted sample volume, and controlling the sampling device to aspirate based on the adjusted sample volume. Due to the influence of altitude pressure, the sample volume actually aspirated by the sampling device after aspirating based on the adjusted sample volume is the same as the required preset sample volume, or in other words, meets the preset sample volume requirement.

[0057] Currently, sample aspiration compensation is typically performed by setting different compensation parameters based on different altitude ranges, or by using different aspiration control software for different altitude ranges. This approach, on the one hand, requires ensuring high consistency in the production and manufacturing processes of the sample aspiration equipment, ensuring that the instrument and piping are as consistent as possible so that they can share a common set of compensation parameters. However, in actual production and manufacturing, variations in production and manufacturing processes inevitably occur, often leading to significant inter-instrumental variation between different sample aspiration equipment. Using the same set of compensation parameters for these equipment can lead to significant variations in aspiration accuracy. Furthermore, due to varying altitudes, local atmospheric pressure also varies. Using the same set of compensation parameters for sample aspiration equipment in different locations can lead to unidirectional deviations in aspiration accuracy. Even if different compensation parameters are used for different altitude ranges, inter-instrumental variation due to the manufacturing process is still unavoidable.

[0058] This study found that the suction compensation parameters can be adaptively determined by the pressure during the suction and exhalation process of the suction device, so as to reduce the inter-device deviation between the suction devices caused by the production process, reduce the sensitivity to altitude differences, and improve the environmental adaptability of the suction device, thereby improving the accuracy of the suction and injection of the suction device.

[0059] Based on this, an embodiment of the present application provides a method for determining sample suction compensation parameters to reduce the inter-device deviation between sample suction devices caused by the production process, and can reduce the sensitivity to altitude differences, improve the environmental adaptability of the sample suction device, and thus improve the accuracy of the sample suction device's liquid suction and injection.

[0060] The method for determining the sample aspiration compensation parameter provided in the embodiment of the present application can be applied to Figure 1The sample aspirating device 100 is shown. The sample aspirating device 100 includes a sample aspirating head 101, a pressure sensor 102 disposed on a liquid aspirating line of the sample aspirating head, and a processor 103 connected to the pressure sensor 102. During the aspirating and dispensing process of the sample aspirating device 100 (i.e., aspirating and dispensing liquid), the gas pressure in the liquid aspirating line connected to the sample aspirating head 101 changes. The pressure sensor 102 collects and outputs the pressure during the aspirating and dispensing process. The processor 103 obtains a pressure curve based on the pressure output by the pressure sensor 102, determines a sample aspirating compensation parameter for the sample aspirating device 100 based on the pressure curve, and further controls the sample aspirating process of the sample aspirating device 100 based on the sample aspirating compensation parameter via an actuator (e.g., a plunger pump, a syringe, or other motion mechanism (not shown)). For example, a preset aspirated volume is adjusted based on the sample aspirating compensation parameter, and the sample aspirating head is controlled to aspirate based on the adjusted sample aspirated volume, so that the actual aspirated volume obtained after aspirating and dispensing matches the preset aspirated volume. The sampling head 101 can be any device that cooperates with the sampling device to perform liquid aspiration and liquid discharge operations, such as a tip head, and the pressure sensor 102 can be implemented by using any pressure device that collects pressure during the aspiration and discharge process.

[0061] refer to Figure 2 As shown, the embodiment of the present application provides a method for determining a sample suction compensation parameter, which is applied to Figure 1 The processor 103 shown in FIG is used as an example for description, wherein the method includes:

[0062] Step S201: obtaining a preset gas volume and a pressure curve of the sample suction and discharge process of the sample suction device.

[0063] The preset gas volume is a system preset value, which can be determined based on the size of the isolation gas column designed by the system and the preset sample addition amount. For example, in some embodiments, the preset gas volume can be the sum of the system preset isolation gas column volume and the preset sample addition amount.

[0064] The sample aspiration and sample discharge process of the sample aspirating device refers to the process of the sample aspirating device aspirating and discharging liquid.

[0065] There is no limit to the method of obtaining the pressure curve of the suction and exhalation process of the sampling device. In some embodiments, the signal collected by the pressure sensor 102 is an analog signal, and the analog signal can be converted into an analog-to-digital signal to obtain an ADC (Analog to Digital Converter) digital signal, and based on the correspondence between the ADC digital signal and the pressure value, the corresponding pressure value is obtained. Based on the multiple pressure values obtained in time sequence, a pressure curve of the suction and exhalation process of the sampling device can be formed.

[0066] Step S202: Based on the pressure curve, an initial pressure value before sample aspiration, a pressure value at the end of sample aspiration, and a pressure value at the end of sample discharge are obtained.

[0067] Because the pressure curve reflects the pressure changes during the aspiration and exhalation process of the sample aspirator, the initial pressure value before aspiration, the pressure value at the end of aspiration, and the pressure value at the end of exhalation can be obtained based on the pressure curve. The initial pressure value before aspiration reflects the local air pressure, that is, the initial state before aspiration; the pressure value at the end of aspiration reflects the pressure value after aspiration (i.e., the state where the sample liquid has been aspirated but not exhaled); and the pressure value at the end of exhalation reflects the pressure value after exhalation (i.e., the state where the sample liquid has been aspirated and exhaled).

[0068] Step S203: according to the relationship between gas volume and pressure, based on the preset gas volume, the initial pressure value before sample aspiration, the pressure value at the end of sample aspiration, and the pressure value at the end of sample discharge, the gas volume at the end of sample aspiration and the gas volume at the end of sample discharge are obtained.

[0069] Under different gas volumes, the gas pressure will also be different. Therefore, based on the relationship between gas volume and pressure, the gas volume at the end of sample aspiration and the gas volume at the end of sample exhalation can be obtained. Among them, the gas volume at the end of sample aspiration reflects the gas volume reflected by the collected gas pressure at the end of sample aspiration, and also reflects the actual volume of liquid in the pipette needle at the end of sample aspiration. The gas volume at the end of sample exhalation reflects the gas volume reflected by the collected gas pressure at the end of sample exhalation, and also reflects the volume of residual liquid in the pipette needle at the end of sample exhalation.

[0070] Step S204: obtaining a sample aspiration compensation parameter based on the sample aspiration end gas volume and the sample exhalation end gas volume.

[0071] The gas volume at the end of aspiration reflects the volume of liquid in the aspiration needle at the end of aspiration, and the gas volume at the end of exhalation reflects the volume of liquid remaining in the aspiration needle at the end of exhalation. Therefore, the aspiration compensation parameter can be determined based on the gas volume at the end of aspiration and the gas volume at the end of exhalation.

[0072] The method for determining the aspiration compensation parameter according to the embodiment of the present application described above can obtain the initial pressure value before aspiration, the pressure value at the end of aspiration, and the pressure value at the end of exhalation during the actual aspiration and exhalation process of the aspiration device based on the pressure curve during the aspiration and exhalation process of the aspiration device. Based on this, the gas volume at the end of aspiration and the gas volume at the end of exhalation can be obtained based on the relationship between gas volume and pressure, and the aspiration compensation parameter can be obtained accordingly. The aspiration compensation parameter obtained in this manner is determined based on the actual aspiration and exhalation process of the aspiration device. The gas volume at the end of aspiration and the gas volume at the end of exhalation determined thereby can reflect the liquid volume at the end of aspiration and the liquid volume after the exhalation. Therefore, based on the gas volume at the end of aspiration and the gas volume at the end of exhalation, the changes in the liquid volume, the liquid volume at the end of aspiration, and the liquid volume after the exhalation process of the aspiration device can be accurately reflected. The aspiration compensation parameter determined thereby can accurately reflect the changes in the liquid volume of the aspiration device during the aspiration and exhalation process. The obtained aspiration compensation parameter has high accuracy. Performing aspiration compensation based on the aspiration compensation parameter can improve the accuracy of the aspiration volume of the aspiration device.

[0073] When obtaining the pressure curve of the aspiration process of the aspirating device, the curve obtained during one aspiration process can be used as the pressure curve, or the pressure curve can be determined by combining multiple aspiration processes. Figure 3 As shown, obtaining the pressure curve of the sample suction device during the sample suction and exhalation process may include:

[0074] Step S2011: obtaining a plurality of initial pressure curves of the sample aspirating and exhaling process performed by the sample aspirating device multiple times.

[0075] The sampling device performs multiple sampling processes, so that each sampling process can obtain a pressure curve, which is called an initial pressure curve in the embodiment of the present application. Multiple sampling processes can obtain multiple initial pressure curves.

[0076] Among them, the specific number of multiple times is not limited and can be set based on the actual technical scenario requirements. For example, it can be a preset value of the default setting, or it can be a value manually set based on actual requirements in a specific technical scenario.

[0077] Step S2013: performing mean processing on the multiple initial pressure curves to obtain the pressure curve.

[0078] There is no limitation on the method of performing the averaging process on the multiple initial pressure curves, as long as the average curve of the multiple initial pressure curves can be calculated.

[0079] Based on this embodiment, when obtaining the pressure curve of the suction and exhalation process of the sampling device, after the sampling device performs multiple suction and exhalation processes, homogenization processing is performed based on the initial pressure curves of multiple suction and exhalation processes to obtain the final pressure curve for analysis, thereby reducing the influence of individual differences in the initial pressure curve of only sucking and exhaling once. The determination of the suction compensation parameter based on the pressure curve after the average processing of multiple initial pressure curves can also make the finally obtained suction compensation parameter more accurate. Suction compensation is performed on this basis, which can further improve the accuracy of suction.

[0080] In some embodiments, as Figure 3 As shown, between the above steps S2011 and S2013, the following steps are also included:

[0081] Step S2012: performing filtering processing on the multiple initial pressure curves.

[0082] There is no limit to the way of filtering the initial pressure curve. By filtering, interference signals in the initial pressure curve can be filtered out, which can improve the accuracy of the obtained pressure curve and help improve the accuracy of the sample suction compensation parameters obtained based on the pressure curve.

[0083] In some embodiments, as Figure 4 As shown, the above step S203, according to the relationship between gas volume and pressure, obtains the gas volume at the end of sample aspiration and the gas volume at the end of sample exhalation based on the preset gas volume, the initial pressure value before sample aspiration, the pressure value at the end of sample aspiration, and the pressure value at the end of sample exhalation, which may include steps S2031 and S2032:

[0084] Step S2031: Substitute the preset gas volume, the initial pressure value before sample aspiration, the pressure value at the end of sample aspiration, and the pressure value at the end of sample discharge into the van der Waals equation to obtain a van der Waals equation group.

[0085] The van der Waals equation, also known as the Van's equation, takes into account the size of gas molecules and the interaction forces between molecules on the basis of the ideal gas equation, thereby better reflecting the macroscopic physical properties of the gas. The van der Waals equation can be expressed as shown in the following equation (1).

[0086] (1)

[0087] In the above formula (1), For pressure, and is the van der Waals constant, is the amount of substance (characterizing the preset volume ,pressure ,temperature the number of gas molecules below), is the volume of gas, also known as the volume of the gas column, is the molar gas constant, is the Kelvin temperature.

[0088] The initial pressure value before aspiration, the pressure value at the end of aspiration, and the pressure value at the end of discharge are recorded as 、 ,and , respectively put them into the above formula (1), we can get formula (2)-(4):

[0089] (2)

[0090] (3)

[0091] (4).

[0092] Combining the above equations (2) to (4), we can obtain the van der Waals equations. is the preset gas volume.

[0093] Step S2032: Solve the van der Waals equations to obtain the gas volume at the end of the sample aspiration and the gas volume at the end of the sample exhalation.

[0094] Based on the above formulas (2) to (4), since the laboratory where the sample suction equipment is located usually has a constant temperature environment, such as 25℃±3℃, the temperature can be regarded as a constant, so in formula (2), except is unknown, the others are known quantities, among which, The preset gas volume is the system preset value, which can be determined based on the size of the isolation gas column designed by the system and the preset sample volume. For example, if the isolation gas column is designed to be 100μL and the test requires the sample to be added to be 50μL, then the V0 value is 150μL, and Determined based on the pressure curve. Therefore, the amount of substance can be calculated based on formula (2) Combining equations (2) and (3) we can calculate the gas volume at the end of the sampling. , combining equations (2) and (4) to calculate the volume of gas at the end of discharge: .

[0095] It should be understood that in some embodiments, Figure 1 As shown, the sample suction device 100 is also provided with a temperature sensor 104, which is in communication with the processor 103. The temperature sensor 104 can be used to collect the temperature in real time. At this time, the temperature The gas volume at the end of the sampling can also be obtained by combining equations (2)-(4) as known values. and the gas volume at the end of spitting .

[0096] Based on this embodiment, after obtaining the initial pressure value before sample aspiration, the pressure value at the end of sample aspiration, and the pressure value at the end of sample exhalation from the pressure curve, since the van der Waals equation takes into account the size of the gas molecules themselves and the interaction force between molecules on the basis of the gas state equation, it better reflects and describes the macroscopic physics of the gas. Therefore, the gas volume at the end of sample aspiration and the gas volume at the end of sample exhalation determined based on this are more accurate. Determining the sample aspiration compensation parameters on this basis can also improve the accuracy of the obtained sample aspiration compensation parameters, and can also further improve the accuracy of the sample aspiration amount of the sample aspiration device.

[0097] Based on the sample aspiration end gas volume and the sample exhalation end gas volume, the method of obtaining the sample aspiration compensation parameter is not limited. In some embodiments, for example, Figure 5 As shown, the above step S204 obtains the sample aspiration compensation parameter based on the sample aspiration end gas volume and the sample exhalation end gas volume, including steps S2041 and S2042:

[0098] Step S2041: Calculating the volume difference between the gas volume at the end of the sample aspiration and the gas volume at the end of the sample exhalation.

[0099] As mentioned above, the gas volume at the end of aspiration is recorded as , the gas volume at the end of spitting out is recorded as , then the volume difference is .

[0100] Step S2042: Determine the sample aspiration compensation parameter based on the volume difference.

[0101] Based on this embodiment, by calculating the volume difference between the gas volume at the end of sample aspiration and the gas volume at the end of sample exhalation, and determining the sample aspiration compensation parameter based on the volume difference, the accuracy of the obtained sample aspiration compensation parameter is improved. Performing sample aspiration compensation based on the sample aspiration compensation parameter can improve the accuracy of the sample aspiration amount of the sample aspiration device.

[0102] The manner of determining the sample aspiration compensation parameter based on the volume difference is not limited. In some embodiments, determining the sample aspiration compensation parameter based on the volume difference includes:

[0103] The volume difference is used as the sample aspiration compensation parameter.

[0104] Therefore, based on this embodiment, by using the volume difference between the gas volume at the end of sample aspiration and the gas volume at the end of sample exhalation as the sample aspiration compensation parameter, it is simple and convenient, and can improve the efficiency of the process of determining the sample aspiration compensation parameter.

[0105] In other embodiments, Figure 6 As shown, the above step S2042 of determining the sample aspiration compensation parameter based on the volume difference may include steps S601 and S602:

[0106] Step S601: determining a correction coefficient based on the gas volume at the end of the sample aspiration and the gas volume at the end of the sample exhalation.

[0107] In some embodiments, determining the correction coefficient based on the gas volume at the end of sample aspiration and the gas volume at the end of sample exhalation may include:

[0108] The correction coefficient is determined based on the sum of the volume difference and a preset gas volume, and based on the ratio of the sum to a base gas volume, where the base gas volume is determined based on the preset gas volume and a preset sample addition amount during the inhalation and exhalation process.

[0109] The volume difference as mentioned above is , the basic gas volume is the above preset gas volume , then the correction coefficient can be expressed as: .

[0110] Based on this embodiment, when determining the correction coefficient, it is determined based on the preset gas volume and the preset sample addition amount, so that it can be determined based on the preset sample addition amount of the aspiration and exhalation process in combination with the obtained volume difference, so that the obtained correction coefficient is determined in combination with the actual aspiration and exhalation process, so that the aspiration compensation parameter determined based on the obtained correction coefficient will be more accurate than directly using the volume difference as the aspiration compensation parameter, thereby further improving the accuracy of the obtained aspiration compensation parameter. Performing aspiration compensation on this basis can also further improve the accuracy of aspiration.

[0111] Step S602: Correcting the volume difference based on the correction coefficient to obtain the sample aspiration compensation parameter.

[0112] Based on this embodiment, a correction coefficient is determined by the gas volume at the end of sample aspiration and the gas volume at the end of sample exhalation, and the volume difference is corrected using the correction coefficient to obtain a final sample aspiration compensation parameter. The obtained sample aspiration compensation parameter can reduce the influence of the error between the obtained gas volume at the end of sample aspiration and the gas volume at the end of sample exhalation, correct the slight error introduced by the sample aspiration compensation parameter, and further improve the accuracy of the obtained sample aspiration compensation parameter. Sample aspiration compensation is performed on this basis, which can also further improve the accuracy of sample aspiration.

[0113] Based on the above embodiments, some specific application examples are given below for illustration.

[0114] After the sample loading device is deployed in a specific application environment, such as a laboratory department, before the specific use of the sample loading device, the sample loading device can be used to perform multiple sample aspiration and expulsion processes based on the method of the embodiment of the present application, and the pressure curve during the sample aspiration and expulsion process can be collected to determine the sample aspiration compensation parameter, and the sample aspiration compensation coefficient can be applied to subsequent sample aspiration and expulsion processes of the sample loading device. Specifically, after the sample loading device has been put into use for a period of time, the method of the embodiment of the present application can be used again at regular intervals, or when it is deemed necessary to re-determine the sample aspiration compensation parameter, to perform multiple sample aspiration and expulsion processes, collect the pressure curve during the sample aspiration and expulsion process, determine the new sample aspiration compensation parameter, and apply it to subsequent sample aspiration and expulsion processes of the sample loading device. Taking into account that in some application processes, the sampling equipment and in vitro diagnostic equipment usually perform many suction and exhalation processes in a day, that is, they are not shut down within a certain continuous working time, therefore, in some application scenarios, each time the sampling equipment is turned on, the method of the embodiment of the present application can be executed first, and multiple suction and exhalation processes are performed, and the pressure curve of the suction and exhalation process is collected to determine the suction compensation parameters during the working process after this startup, so that the obtained suction compensation parameters are more in line with the working conditions of this startup, and the accuracy of the obtained suction compensation parameters can be improved.

[0115] It is understandable that the process of determining the sample suction compensation parameter can be completed by the cooperation of software and the sample suction device. For example, a sample suction compensation parameter control is provided on the application interface of the software, and the user issues an instruction by clicking the control, thereby automatically executing the process of adding and discharging the sample and determining the sample suction compensation parameter. A physical button can also be provided on the sample suction device, and the user issues an instruction by operating the button, thereby automatically executing the process of adding and discharging the sample and determining the sample suction compensation parameter. It is understandable that instructions can also be issued through other operating methods, and the embodiments of the present application do not impose specific restrictions on this.

[0116] When it is necessary to determine the sampling compensation parameters of the sampling device, the sampling device performs a sampling and expelling process, and collects the ADC (analog-to-digital conversion) signal output by the pressure sensor 102 during the sampling and expelling process. Based on the relationship between the output signal of the pressure sensor and pressure, the signal is converted into a pressure signal. Then, based on the multiple pressure signals in a time series, an initial pressure curve C of the sampling and expelling process is formed. The initial pressure curve C is filtered to remove interference signals and obtain a pressure curve Ci.

[0117] The above-mentioned sample aspiration and sample discharge process is performed multiple times to obtain multiple pressure curves C{i} (=C1, ..., Cn). The n pressure curves C{i} obtained above are processed by the average algorithm to obtain the local standard pressure curve Co{i}. A schematic diagram of the pressure curve Co{i} in a specific example is shown as follows Figure 7 As shown, the initial pressure value before aspiration can be obtained (the pressure value at the end of sample addition is equivalent to the pressure value shown in the figure) , pressure value at the end of sampling , Sample discharge end pressure value , they reflect the state of the aspirated liquid of the aspirating needle as follows Figure 8 shown.

[0118] There is no limitation on the way to obtain the initial pressure value before aspirating, the pressure value at the end of aspirating, and the pressure value at the end of discharging. A pressure value in the stable area after the addition of the sample can be used as the initial pressure value before aspirating, such as Figure 3 As shown in The average of multiple pressure values in the stable area after the sampling is completed can also be used as the pressure value at the end of the sampling. A pressure value in the stable area after the sampling is completed can be used as the sampling end pressure value, such as Figure 3 As shown in The average of multiple pressure values in the stable area after the sampling is completed can also be used as the pressure value at the end of the sampling. A pressure value in the stable area after the sample is ejected can be used as the sample end pressure value, such as Figure 3 As shown in The average of multiple pressure values in the stable area after the sample is ejected can also be used as the end pressure value of the sample. .

[0119] Set the preset gas volume , preset gas volume initial pressure value before sampling , pressure value at the end of sampling , Sample discharge end pressure value Substituting into the van der Waals equation (1) respectively, we can obtain the equation system composed of equations (2), (3), and (4). The combined equations (2), (3), and (4) can be used to calculate the gas volume at the end of the sample aspiration. and the gas volume at the end of spitting .

[0120] The gas volume at the end of the aspiration is obtained and the gas volume at the end of spitting Then, the volume difference can be calculated .

[0121] Obtaining the volume difference Then, in some embodiments, the volume difference can be directly In other embodiments, the sample aspiration compensation parameter may be determined based on the correction coefficient after the correction coefficient is calculated.

[0122] Taking the calculation of the correction coefficient and the determination of the sample suction compensation parameter based on the correction coefficient as an example, by introducing the correction coefficient, the slight error introduced by the sample suction compensation parameter can be corrected to obtain a more accurate compensation amount and high-precision compensation. , then the pipetting error is , the spitting error is , then the difference between suction and injection is , where the correction factor Can be used Approximate solution, As a correction factor .

[0123] In some embodiments, the correction coefficient can also be obtained by iterative calculation Specifically, based on the sample compensation parameters determined this time After the sample is sucked and compensated, the preset gas volume Adjustment( ), so that the gas volume at the end of sampling and the gas volume at the end of spitting , changes to , , and then determine the correction coefficient and sampling compensation parameters again, and iterate continuously until the convergence accuracy meets the requirements. It should be understood that when Much smaller than When , it can be approximated ,at this time .

[0124] After determining the above sampling compensation parameters, when applied to a specific sampling process, sampling compensation can be performed based on the sampling compensation parameters. Assuming that the preset sampling volume input by the user is , then use the sampling compensation parameter After the sample aspiration compensation is performed, the target sample aspiration volume (i.e. the actual sample aspiration volume of the motion mechanism) is The actual amount of sample added is , thereby being able to adaptively compensate for the effect of ambient pressure on the sample volume.

[0125] Based on the method of the embodiment of the present application as described above, since the sample suction device is generally provided with a pressure sensor to realize the detection of blocked needle / empty needle, when performing pressure detection, it can be directly based on the existing pressure sensor, without the need to add any new hardware, and can be realized only by software processing, thus saving hardware costs. In the process of determining the sample suction compensation parameters, it is not necessary to consider the differences between different sample suction devices, which reduces the requirements for production and manufacturing, thereby reducing production costs, and there is no need to maintain multiple different versions of software programs, which also reduces the cost of software development and maintenance. And because the sample suction compensation parameters are determined based on the sample addition and sample discharging process of the sample adding device itself, the differences between different sample suction devices are reduced, the sample addition accuracy is improved, and the credibility of the detection results of the in vitro detection device is improved.

[0126] It should be understood that, although the various steps in the flowcharts involved in the various embodiments described above are displayed in sequence according to the instructions of the arrows, these steps are not necessarily executed in sequence in the order indicated by the arrows. Unless otherwise specified herein, there is no strict order restriction on the execution of these steps, and these steps can be executed in other orders. Moreover, at least a portion of the steps in the flowcharts involved in the various embodiments described above can include multiple steps or multiple stages, and these steps or stages are not necessarily executed and completed at the same time, but can be executed at different times, and the execution order of these steps or stages is not necessarily to be carried out in sequence, but can be executed in turn or alternately with other steps or at least a portion of steps or stages in other steps.

[0127] Based on the same inventive concept, embodiments of the present application also provide a device for determining a sample aspiration compensation parameter for implementing the aforementioned method for determining a sample aspiration compensation parameter. The solution provided by this device is similar to the solution described in the aforementioned method. Therefore, the specific limitations of one or more embodiments of the device for determining a sample aspiration compensation parameter provided below can be found in the limitations of the method for determining a sample aspiration compensation parameter described above and will not be further elaborated here.

[0128] In one embodiment, Figure 9 As shown, a device for determining a sample aspiration compensation parameter is provided, comprising: a curve acquisition module 901, a pressure value acquisition module 902, a volume parameter determination module 903, and a compensation parameter determination module 904, wherein:

[0129] The information acquisition module 901 is used to obtain the preset gas volume and the pressure curve of the sample suction and exhalation process of the sample suction device;

[0130] A pressure value acquisition module 902 is configured to obtain an initial pressure value before sample aspiration, a pressure value at the end of sample aspiration, and a pressure value at the end of sample discharge based on the pressure curve;

[0131] The volume parameter determination module 903 is configured to obtain the gas volume at the end of sample aspiration and the gas volume at the end of sample exhalation based on the relationship between gas volume and pressure, the preset gas volume, the initial pressure value before sample aspiration, the pressure value at the end of sample aspiration, and the pressure value at the end of sample exhalation;

[0132] The compensation parameter determination module 904 is configured to obtain a sample aspiration compensation parameter based on the sample aspiration end gas volume and the sample exhalation end gas volume.

[0133] In some embodiments, the volume parameter determination module 903 is used to respectively substitute the initial pressure value before sample aspiration, the pressure value at the end of sample aspiration, and the pressure value at the end of sample exhalation into the van der Waals equation to obtain a van der Waals equation group; and solve the van der Waals equation group to obtain the gas volume at the end of sample aspiration and the gas volume at the end of sample exhalation.

[0134] In some embodiments, the compensation parameter determination module 904 is configured to calculate a volume difference between the gas volume at the end of the sample aspiration and the gas volume at the end of the sample exhalation; and determine the sample aspiration compensation parameter based on the volume difference.

[0135] In some embodiments, the compensation parameter determination module 904 is further configured to use the volume difference as the sample aspiration compensation parameter.

[0136] In some embodiments, the compensation parameter determination module 904 is further configured to determine a correction coefficient based on the gas volume at the end of sample aspiration and the gas volume at the end of sample exhalation; and correct the volume difference based on the correction coefficient to obtain the sample aspiration compensation parameter.

[0137] In some embodiments, the compensation parameter determination module 904 is configured to determine the correction coefficient based on a ratio of the sum of the volume difference and a preset gas volume to a base gas volume, where the base gas volume is determined based on the preset gas volume and a preset sample addition amount during the aspiration and exhalation process.

[0138] In some embodiments, the curve acquisition module 901 is configured to acquire a plurality of initial pressure curves of the sample aspirating device during multiple aspiration and exhalation processes; and perform mean processing on the plurality of initial pressure curves to obtain the pressure curve.

[0139] Each module in the aforementioned apparatus for determining the sample aspiration compensation parameter may be implemented in whole or in part via software, hardware, or a combination thereof. Each module may be embedded in or independent of a processor within a computer device in the form of hardware, or may be stored in a memory within the computer device in the form of software, so that the processor can call and execute the corresponding operations of each module.

[0140] In one embodiment, a sample suction device is provided, such as Figure 1As shown, the sample suction device 100 includes a sample suction head 101, a pressure sensor 102 arranged on the liquid suction pipeline of the sample suction head 101, and a processor 103 communicatively connected to the pressure sensor 102, wherein the pressure sensor 102 collects and outputs the pressure of the sample suction device during the sample suction and exhalation process, the processor obtains a pressure curve based on the pressure output by the pressure sensor, and determines the sample suction compensation parameters of the sample suction device based on the method in any of the embodiments described above.

[0141] In some embodiments, based on practical needs, the sample aspiration device may further include a memory, a communication interface, a display screen, and an input device (none of which are shown in the figures). The processor is used to provide computing and control capabilities. The memory includes a non-volatile storage medium and internal memory. The non-volatile storage medium stores an operating system and computer programs, and the internal memory provides an environment for the operation of the operating system and computer programs in the non-volatile storage medium. The communication interface is used for wired or wireless communication with external devices. Wireless communication can be achieved through Wi-Fi, mobile cellular networks, NFC (near-field communication), or other technologies. The processor can execute the computer program stored in the memory to implement a method for determining sample aspiration compensation parameters. The display screen can be a liquid crystal display or an electronic ink display screen. The input device of the computer device can be a touch screen layer covering the display screen, keys, trackball, or touchpad provided on the computer device housing, or an external keyboard, touchpad, or mouse.

[0142] In one embodiment, the processor 103 is further configured to obtain a preset sample aspiration volume, determine a target sample aspiration volume based on the preset sample aspiration volume and the sample aspiration compensation parameter, and control the sample aspiration head to aspirate samples based on the target sample aspiration volume.

[0143] In one embodiment, a computer-readable storage medium is provided, on which a computer program is stored. When the computer program is executed by a processor, the steps of the method for determining the sample aspiration compensation parameter in any of the above embodiments are implemented.

[0144] In one embodiment, a computer program product is provided, comprising a computer program, which, when executed by a processor, implements the steps of the method for determining the sample aspiration compensation parameter in any of the above-described embodiments.

[0145] Those skilled in the art will appreciate that all or part of the processes in the above-mentioned embodiments can be implemented by instructing the relevant hardware through a computer program. The computer program can be stored in a non-volatile computer-readable storage medium. When the computer program is executed, it can include the processes of the above-mentioned embodiments. In particular, any reference to memory, database, or other media used in the embodiments provided in this application can include at least one of non-volatile and volatile memory. Non-volatile memory can include read-only memory (ROM), magnetic tape, floppy disk, flash memory, optical memory, high-density embedded non-volatile memory, resistive random access memory (ReRAM), magnetic random access memory (MRAM), ferroelectric random access memory (FRAM), phase change memory (PCM), graphene memory, etc. Volatile memory can include random access memory (RAM) or external cache memory, etc. By way of illustration and not limitation, RAM can take various forms, such as static random access memory (SRAM) or dynamic random access memory (DRAM). The databases involved in the various embodiments provided herein may include at least one of a relational database and a non-relational database. Non-relational databases may include, but are not limited to, distributed databases based on blockchains. The processors involved in the various embodiments provided herein may be, but are not limited to, general-purpose processors, central processing units (CPUs), graphics processing units (GPUs), digital signal processors (DSPs), programmable logic devices (PLDs), data processing logic devices based on quantum computing, and the like.

[0146] The technical features of the above embodiments can be combined arbitrarily. To make the description concise, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0147] The above-described embodiments merely represent several implementation methods of the present application. While the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the present application. It should be noted that a person of ordinary skill in the art may make various modifications and improvements without departing from the spirit of the present application, and these modifications and improvements fall within the scope of protection of the present application. Therefore, the scope of protection of the present application shall be determined by the appended claims.

Claims

1. A method for determining a sample aspiration compensation parameter, characterized in that: The method comprises: Obtain the preset gas volume and the pressure curve of the sampling device during the sampling process; Based on the pressure curve, an initial pressure value before sample aspiration, a pressure value at the end of sample aspiration, and a pressure value at the end of sample discharge are obtained; Substituting the preset gas volume, the initial pressure value before sample aspiration, the pressure value at the end of sample aspiration, and the pressure value at the end of sample discharge into the van der Waals equation respectively to obtain a van der Waals equation group; Solving the van der Waals equations to obtain the gas volume at the end of sample aspiration and the gas volume at the end of sample exhalation; Calculate the volume difference between the gas volume at the end of aspiration and the gas volume at the end of exhalation; The sample aspiration compensation parameter is determined based on the volume difference.

2. The method according to claim 1, characterized in that The determining of the sample aspiration compensation parameter based on the volume difference includes: The volume difference is used as the sample aspiration compensation parameter.

3. The method according to claim 1, characterized in that The step of obtaining a pressure curve of a sample suction and exhalation process of the sample suction device includes: Acquiring multiple initial pressure curves of the sample suction device during multiple sample suction and exhalation processes; Averaging is performed on the multiple initial pressure curves to obtain the pressure curve.

4. The method according to claim 1, wherein The determining of the sample aspiration compensation parameter based on the volume difference includes: determining a correction coefficient based on the gas volume at the end of the sample aspiration and the gas volume at the end of the sample exhalation; The volume difference is corrected based on the correction coefficient to obtain the sample aspiration compensation parameter.

5. The method according to claim 4, characterized in that The determining of the correction coefficient based on the gas volume at the end of the sample aspiration and the gas volume at the end of the sample exhalation includes: The correction coefficient is determined based on the ratio of the sum of the volume difference and the preset gas volume to the basic gas volume, where the basic gas volume is determined based on the preset gas volume and the preset sample addition amount during the suction and exhalation process.

6. A device for determining a sample aspiration compensation parameter, characterized in that: The device comprises: A curve acquisition module is used to obtain the preset gas volume and the pressure curve of the suction and exhalation process of the sampling device; A pressure value acquisition module, configured to obtain an initial pressure value before sample aspiration, a pressure value at the end of sample aspiration, and a pressure value at the end of sample discharge based on the pressure curve; a volume parameter determination module, configured to substitute the preset gas volume, the initial pressure value before sample aspiration, the pressure value at the end of sample aspiration, and the pressure value at the end of sample exhalation into a van der Waals equation to obtain a van der Waals equation system; and solve the van der Waals equation system to obtain a gas volume at the end of sample aspiration and a gas volume at the end of sample exhalation; The compensation parameter determination module is used to calculate the volume difference between the gas volume at the end of the sample aspiration and the gas volume at the end of the sample exhalation; and determine the sample aspiration compensation parameter based on the volume difference.

7. A sample suction device, comprising a sample suction head, a pressure sensor provided on a liquid suction line of the sample suction head, and a processor connected to the pressure sensor, characterized in that: The pressure sensor collects and outputs the pressure of the sample suction device during the sample suction and exhalation process, the processor obtains a pressure curve based on the pressure output by the pressure sensor, and determines the sample suction compensation parameter of the sample suction device based on the method described in any one of claims 1 to 5.

8. The sample suction device according to claim 7, characterized in that: The processor is further configured to obtain a preset sample aspiration volume, determine a target sample aspiration volume based on the preset sample aspiration volume and the sample aspiration compensation parameter, and control the sample aspiration head to aspirate samples based on the target sample aspiration volume.

9. A computer-readable storage medium storing a computer program, characterized in that: When the computer program is executed by a processor, the steps of the method according to any one of claims 1 to 5 are implemented.

10. A computer program product comprising a computer program, characterized in that When the computer program is executed by a processor, the steps of the method according to any one of claims 1 to 5 are implemented.

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