A split control device, split device and split method

Abstract

The present application relates to the technical field of cell sub-packaging, and discloses a sub-packaging control device, a sub-packaging equipment and a sub-packaging method. The sub-packaging control device comprises a conveying component, a sub-packaging component, a flow detection component and a controller. The conveying component comprises a first pump body, a first control valve, a second control valve and an air inlet pipeline. The sub-packaging component comprises a plurality of sub-packaging control valves, a branch pipeline in communication with a main pipeline and a sub-packaging pipeline in communication with the plurality of branch pipelines. The flow detection component comprises a second pump body, a flow detection element and the main pipeline. The second pump body is arranged in the main pipeline. The flow detection element is connected in series to the main pipeline and located between the second pump body and the branch pipeline. The controller is electrically connected to the first pump body, the first control valve, the second control valve, the sub-packaging control valves, the second pump body and the flow detection element, respectively. The sub-packaging control device can effectively improve the sub-packaging precision and the sub-packaging efficiency of cell sub-packaging, thereby improving the survival rate of cells and avoiding the adverse effects of cell functions in the sub-packaging process.

Description

Technical Field

[0001] The present invention relates to the technical field of cell packaging, and in particular to a packaging control device, packaging equipment and a packaging method. Background Art

[0002] Aliquoting is a crucial step in the cell preparation process, crucial for volume and density uniformity, as well as cell recovery, viability, and function prior to cryopreservation. However, current cell aliquoting equipment suffers from low aliquoting accuracy, negatively impacting cell viability and function. Summary of the Invention

[0003] The present invention provides a packaging control device, which can effectively improve the packaging accuracy and packaging efficiency of cell packaging, thereby increasing the survival rate of cells and preventing cell functions from being adversely affected during the packaging process.

[0004] The invention also provides a subpackaging device.

[0005] The invention also provides a subpackaging method.

[0006] According to an embodiment of the first aspect of the present invention, a subpackaging control device is provided, comprising:

[0007] The flow detection component includes a second pump body, a flow detection element and a main line, wherein the second pump body is arranged in the main line; the flow detection element is connected in series with the main line and is located in the downstream direction of the second pump body;

[0008] The delivery component includes a first pump body, a first control valve, a second control valve, and a pipeline assembly, wherein the pipeline assembly includes a first pipeline, a second pipeline, and an air intake pipeline, wherein the first pipeline is respectively connected to the sample bag and the mixing bag, and the first pump body is arranged in the first pipeline; the second pipeline is respectively connected to the first pipeline and the air intake pipeline, the first control valve is arranged in the second pipeline, and the second control valve is arranged in the air intake pipeline;

[0009] The sub-packaging component includes a plurality of sub-packaging control valves, branch lines connected to the main line, and a plurality of sub-packaging pipes connected to the branch lines, wherein the plurality of sub-packaging control valves are provided in a one-to-one correspondence with the plurality of sub-packaging pipes, and the plurality of sub-packaging pipes are connected to the preparation bags in a one-to-one correspondence;

[0010] The controller is electrically connected to the first pump body, the first control valve, the second control valve, the sub-packaging control valve, the second pump body and the flow detection element respectively.

[0011] According to a subpackaging control device provided by an embodiment of the present invention, the flow detection component further includes:

[0012] a drip bucket, connected in series to the main pipe and located between the second pump body and the first control valve;

[0013] The drip bucket rotating member is connected to the drip bucket, the drip bucket rotating member is electrically connected to the controller, and the drip bucket rotating member is used to drive the drip bucket to rotate.

[0014] According to a packaging control device provided by an embodiment of the present invention, the flow detection component also includes a first bubble sensor and a second bubble sensor, the first bubble sensor is arranged in the first pipeline and is located between the first pump body and the mixing bag; the second bubble sensor is arranged in the main pipeline and is located between the flow detection component and the second pump body; the first bubble sensor and the second bubble sensor are both electrically connected to the controller.

[0015] According to a subpackaging control device provided by an embodiment of the present invention, the flow detection component further includes:

[0016] a third bubble sensor electrically connected to the controller, the third bubble sensor being disposed in the main pipe and located between the flow detection element and the branch pipe;

[0017] The flow detection component is a quantitative tube, and the flow detection component further includes:

[0018] The quantitative tube rotating member is connected to the quantitative tube, the quantitative tube rotating member is electrically connected to the controller, and the quantitative tube rotating member is used to drive the quantitative tube to rotate.

[0019] According to an embodiment of the present invention, a subpackaging control device is provided, wherein the subpackaging component further includes:

[0020] a fourth bubble sensor, electrically connected to the controller, and disposed at one end of the branch pipe close to the main pipe;

[0021] An air pressure sensor is electrically connected to the controller, and the air pressure sensor is arranged at one end of the branch pipe away from the main pipe.

[0022] According to an embodiment of the present invention, a subpackaging control device is provided, which includes a first subpackaging component and a second subpackaging component. The first subpackaging component includes a first branch pipeline, multiple first subpackaging control valves and multiple first subpackaging pipes connected to the first branch pipeline, and the multiple first subpackaging control valves are arranged in a one-to-one correspondence with the multiple first subpackaging pipes; the second subpackaging component includes a second branch pipeline, multiple second subpackaging control valves and multiple second subpackaging pipes connected to the second branch pipeline, and the multiple second subpackaging control valves are arranged in a one-to-one correspondence with the multiple second subpackaging pipes.

[0023] According to an embodiment of the second aspect of the present invention, a subpackaging device is provided, comprising a body and any one of the above-mentioned subpackaging control devices, wherein the subpackaging control device is arranged in the body.

[0024] According to a third aspect of the present invention, an embodiment provides a subpackaging method, which is based on any one of the above-mentioned subpackaging control devices, and includes:

[0025] Step S10, controlling the first pump body and the second pump body to operate so that the cell fluid fills the main pipe and the branch pipe;

[0026] Step S20, sequentially controlling the multiple filling control valves of each filling component to open in a predetermined order for a first predetermined time, so that the remaining volume of the preparation bag corresponding to each filling component is equal to the cell fluid volume in the corresponding pipeline;

[0027] Step S30, controlling the first control valve to close and the second control valve to open;

[0028] Step S40 , sequentially controlling the sub-packaging control valves corresponding to each group of preparation bags that are not filled with cell fluid to open for a second predetermined time, so that the cell fluid in the corresponding pipeline is filled into the corresponding preparation bag that is not filled with cell fluid.

[0029] According to an embodiment of the present invention, a subpackaging method further includes the following steps after executing step S30:

[0030] Step S43: After the second control valve is opened for a third predetermined time, the drip bucket rotating member is controlled to drive the drip bucket to rotate at a first predetermined speed, so that all the cell fluid in the drip bucket flows to the second pump body.

[0031] According to an embodiment of the present invention, a subpackaging method further includes the following steps after executing step S43:

[0032] When it is determined that no cell fluid begins to flow through the second bubble sensor and cell fluid begins to flow through the third bubble sensor, or after a fourth predetermined time has passed after it is determined that no cell fluid begins to flow through the second bubble sensor, the quantitative tube rotating member is controlled to drive the quantitative tube to rotate at a second predetermined speed so that all the cell fluid in the quantitative tube enters the branch line.

[0033] The above one or more technical solutions in the embodiments of the present invention have at least one of the following technical effects:

[0034] According to the packaging control device provided by an embodiment of the present invention, the controller controls the operation of the first pump body to drive the sample liquid in the sample bag to enter the first pipeline, and the sample liquid is mixed with the liquid in the mixing bag in the second pipeline to obtain cell fluid, and the second pump body drives the cell fluid to fill the main pipeline and the branch pipeline; the controller sequentially controls the multiple packaging control valves of each packaging component to open in sequence for a first predetermined time in a predetermined order, so that the remaining capacity of the preparation bag corresponding to each packaging component is equal to the cell fluid capacity in the corresponding pipeline; the controller sequentially controls the packaging control valves corresponding to each group of preparation bags that are not filled with cell fluid to open in sequence for a second predetermined time, so that the cell fluid in the corresponding pipeline is loaded into the corresponding preparation bag that is not filled with cell fluid, ensuring that the cell fluid remaining in the pipeline completely enters the corresponding preparation bag, avoiding cell fluid waste, improving the packaging accuracy and packaging efficiency of cell packaging, thereby improving the survival rate of cells, and avoiding adverse effects on cell function during the packaging process.

[0035] Additional aspects and advantages of the present invention will be set forth in part in the description which follows and, in part, will be obvious from the description which follows, or may be learned by practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] In order to more clearly illustrate the technical solutions in the present invention or the prior art, a brief introduction will be given below to the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0037] Figure 1 Schematic diagram of the structure of the packaging control device provided by an embodiment of the present invention;

[0038] Figure 2 This is a structural diagram of the assembly relationship between the first pump body, the second pump body and the third sub-assembly panel provided in an embodiment of the present invention;

[0039] Figure 3 This is a structural diagram of the assembly relationship between the quantitative tube rotating member, the second bubble sensor, the third bubble sensor and the second filling panel provided by an embodiment of the present invention;

[0040] Figure 4 1 is a schematic structural diagram of the assembly relationship between the first sub-assembly component and the first sub-assembly panel provided in an embodiment of the present invention;

[0041] Figure 5 It is a flow chart of the subpackaging control method provided by an embodiment of the present invention.

[0042] Reference numerals:

[0043] 110. First pump body; 120. First control valve; 130. Second control valve; 140. Sample bag; 150. Mixing bag; 160. First pipeline; 170. Second pipeline; 180. Air inlet pipeline; 210. Second pump body; 220. Flow detection component; 221. Quantitative tube rotating component; 230. Drip bucket; 231. Drip bucket rotating component; 240. First bubble sensor; 250. Second bubble sensor; 260. Third bubble sensor; 270. Main pipeline; 310. Air pressure sensor; 320. Fourth bubble sensor; 341. First branch pipeline; 342. First sub-packaging control valve; 351. Second branch pipeline; 352. Second sub-packaging control valve; 410. First sub-packaging panel; 420. Second sub-packaging panel; 430. Third sub-packaging panel; 500. Preparation bag. DETAILED DESCRIPTION

[0044] The following embodiments of the present invention are described in further detail with reference to the accompanying drawings and examples. The following examples are used to illustrate the present invention but are not intended to limit the scope of the present invention.

[0045] In the description of the embodiments of the present invention, it should be noted that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing the embodiments of the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore should not be understood as limiting the embodiments of the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only and should not be understood as indicating or implying relative importance.

[0046] In the description of the embodiments of the present invention, it should be noted that, unless otherwise specified or limited, the terms "connected" and "connection" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integral connections; mechanical connections, electrical connections; and direct connections or indirect connections through an intermediary. Those skilled in the art will understand the specific meanings of the above terms in the embodiments of the present invention based on the specific circumstances.

[0047] In the embodiments of the present invention, unless otherwise expressly specified or limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediate medium. Furthermore, a first feature being "above," "above," or "above" a second feature may mean that the first feature is directly above or diagonally above the second feature, or simply means that the first feature is at a higher level than the second feature. A first feature being "below," "below," or "below" a second feature may mean that the first feature is directly below or diagonally below the second feature, or simply means that the first feature is at a lower level than the second feature.

[0048] In the description of this specification, the reference terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" mean that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the embodiment of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art can combine and combine different embodiments or examples described in this specification and features of different embodiments or examples without contradiction.

[0049] The following combination Figures 1 to 4 The following describes a packaging control device provided by an embodiment of the present invention.

[0050] Figure 1 The structural diagram of the packaging control device provided by the embodiment of the present invention is illustrated as follows. Figure 1 As shown, the packaging control device includes a conveying component, a packaging component, a flow detection component and a controller.

[0051] The flow detection component includes a second pump body 210, a flow detection element 220 and a main line 270. The second pump body 210 is disposed on the main line 270. Specifically, the second pump body 210 is clamped on the main line 270. The flow detection element 220 is connected in series with the main line 270 and is located downstream of the second pump body 210.

[0052] The delivery component includes a first pump body 110, a first control valve 120, a second control valve 130, and a pipeline assembly. The pipeline assembly includes a first pipeline 160, a second pipeline 170, and an air intake pipeline 180. The first pump body 110 is disposed in the first pipeline 160. Specifically, the first pump body 110 is clamped to the first pipeline 160. The first pipeline 160 is connected to the sample bag 140 and the mixing bag 150 respectively. Driven by the first pump body 110, the sample liquid in the sample bag 140 enters the first pipeline 160, and the liquid in the mixing bag 150 enters the first pipeline 160 under the action of its own gravity. The second pipeline 170 is connected to the first pipeline 160 and the air intake pipeline 180 respectively. The first control valve 120 is disposed in the second pipeline 170. The sample liquid in the sample bag 140 and the liquid in the mixing bag 150 are mixed in the second pipeline 170 to obtain cell fluid. The second control valve 130 is disposed in the air intake pipeline 180. In this embodiment, the first control valve 120 is clamped to the second pipeline 170, and the second control valve 130 is clamped to the air intake pipeline 180.

[0053] The subpackaging components include multiple subpackaging control valves, branch lines connected to the main line 270, and subpackaging tubes connected to the multiple branch lines. The multiple subpackaging control valves are arranged on the multiple subpackaging tubes in a one-to-one correspondence. Specifically, the multiple subpackaging control valves are clamped on the multiple subpackaging tubes in a one-to-one correspondence, and the multiple subpackaging tubes are connected to the preparation bags 500 in a one-to-one correspondence.

[0054] The controller is electrically connected to the first pump body 110 , the first control valve 120 , the second control valve 130 , the sub-packaging control valve, the second pump body 210 and the flow detection element 220 , respectively.

[0055] According to the packaging control device provided by an embodiment of the present invention, the controller controls the operation of the first pump body 110 to drive the sample liquid in the sample bag 140 into the first pipeline 160, and the sample liquid is mixed with the liquid in the mixing bag 150 in the second pipeline 170 to obtain cell fluid, and the second pump body 210 drives the cell fluid to fill the main pipeline 270 and the branch pipeline; the controller sequentially controls the multiple packaging control valves of each packaging component to open in sequence for a first predetermined time in a predetermined order, so that the remaining capacity of the preparation bag 500 corresponding to each packaging component is equal to the cell fluid capacity in the corresponding pipeline; the controller sequentially controls the packaging control valves corresponding to each group of preparation bags 500 that are not filled with cell fluid to open in sequence for a second predetermined time, so that the cell fluid in the corresponding pipeline is loaded into the corresponding preparation bag 500 that is not filled with cell fluid, ensuring that the cell fluid remaining in the pipeline completely enters the corresponding preparation bag 500, avoiding cell fluid waste, improving the packaging accuracy and packaging efficiency of cell packaging, thereby improving the survival rate of cells, and avoiding adverse effects on cell function during the packaging process.

[0056] In an embodiment of the present invention, the air intake line 180 is connected to the main line 270, and the end of the air intake line 180 away from the second line 170 is connected to the outside atmosphere. When the second control valve 130 in the air intake line 180 is in an open state, the outside air can be pumped into the air intake line 180 and the main line 270 under the action of the second pump body 210 to push the cell fluid inside the main line 270 to flow toward the preparation bag 500.

[0057] In an embodiment of the present invention, Figure 4 The schematic diagram of the assembly relationship between the first sub-assembly component and the first sub-assembly panel provided in the embodiment of the present invention is illustrated as follows: Figure 1 and Figure 4 As shown, the sub-packaging control device includes a first sub-packaging component and a second sub-packaging component. The first sub-packaging component includes a first branch pipeline 341, a plurality of first sub-packaging control valves 342 and a plurality of first sub-packaging pipes connected to the first branch pipeline 341. The plurality of first sub-packaging control valves 342 are arranged in a one-to-one correspondence with the plurality of first sub-packaging pipes. When the cell fluid fills the main pipeline 270 and the branch pipeline, that is, when the cell fluid fills the main pipeline 270 and the first branch pipeline 341, the cell fluid is filled in a predetermined order (the predetermined order in this embodiment is Figure 1 Each first filling control valve 342 is controlled to open for a first predetermined time in sequence (from left to right in the figure), so that the cell fluid is filled into the preparation bag 500 corresponding to each first filling control valve 342 in a predetermined order, and the first filling control valve 342 is closed after the first predetermined time.

[0058] It should be noted that when the preparation bags 500 in the first branch line 341 are being sub-packed, one or more first sub-packaging control valves 342 located at the end of the first branch line 341 away from the main line 270 are in a closed state. At this time, the preparation bags 500 corresponding to the one or more first sub-packaging control valves 342 located at the end of the first branch line 341 away from the main line 270 are not involved in the sub-packaging or the above-mentioned preparation bags 500 are only partially filled. The predetermined order is not limited to Figure 1 The order from left to right can also be Figure 1 In right-to-left order.

[0059] The second sub-packaging component includes a second branch pipeline 351, a plurality of second sub-packaging control valves 352 and a plurality of second sub-packaging pipes connected to the second branch pipeline 351. The plurality of second sub-packaging control valves 352 are respectively provided on the plurality of second sub-packaging pipes. After the sub-packaging of the preparation bag 500 corresponding to the first branch pipeline 341 is completed, the preparation bag 500 is packed in a predetermined order (the predetermined order in this embodiment is Figure 1 Each second packaging control valve 352 is controlled to open for a first predetermined time in sequence (from left to right in the figure), so that the cell fluid is packaged into the preparation bag 500 corresponding to the second packaging control valve 352 in a predetermined order, and the second packaging control valve 352 is closed after the first predetermined time.

[0060] It should be noted here that when the preparation bags 500 of the second branch pipeline 351 are subpacked, one or more second subpackaging control valves 352 located at the end of the second branch pipeline 351 away from the main pipeline 270 are in a closed state. At this time, the preparation bags 500 corresponding to the one or more second subpackaging control valves 352 located at the end of the second branch pipeline 351 away from the main pipeline 270 do not participate in the subpackaging.

[0061] It should also be noted here that the first predetermined time corresponding to the first packaging control valve 342 or the second packaging control valve 352 can be the same or different, and is specifically determined by the capacity of the corresponding preparation bag 500 to be packaged and the flow rate of the cell fluid in the main line 270.

[0062] After the second branch line 351 is filled with the preparation bags 500, the first control valve 120 is controlled to close, while the second control valve 130 is controlled to open. The second pump body 210 is activated by the controller. Driven by the second pump body 210, air enters the main line 270 from the air inlet line 180 and pushes the residual cell fluid in the main line 270 into the branch line, thereby emptying the main line 270. After the main line 270 is emptied, the one or more first filling control valves 342 at the end of the first branch line 341 away from the main line 270 are controlled to open in sequence for a second predetermined time (after which the first filling control valves 342 are closed), so that the residual cell fluid in the main line 270 and the first branch line 341 is filled into the preparation bags 500 corresponding to the one or more first filling control valves 342 at the end of the first branch line 341 away from the main line 270.

[0063] After the cell sap remaining in the main line 270 and the first branch line 341 is drained, the one or more second dispensing control valves 352 at the end of the second branch line 351 remote from the main line 270 are sequentially opened for a second predetermined time, so that the cell sap remaining in the second branch line 351 is dispensed into the preparation bags 500 corresponding to the one or more second dispensing control valves 352 at the end of the second branch line 351 remote from the main line 270. By draining the cell sap remaining in the main line 270 and the branch lines, the waste of cell sap in the main line 270 and the branch lines is effectively reduced.

[0064] It should be noted that the volume of undispensed cell sap in the main line 270 and the first branch line 341 is equal to the remaining volume of the preparation bag 500 not filled with cell sap corresponding to the first branch line 341, and the volume of undispensed cell sap in the second branch line 351 is equal to the volume of the preparation bag 500 not filled with cell sap corresponding to the second branch line 351. By sequentially controlling the opening of the dispensing control valve corresponding to each group of preparation bags 500 not filled with cell sap for a second predetermined time, the cell sap in the corresponding line is dispensed into the corresponding preparation bag 500 not filled with cell sap, ensuring that the cell sap remaining in the line completely enters the corresponding preparation bag 500, thereby avoiding cell sap waste and improving the dispensing accuracy and efficiency of cell dispensing.

[0065] It should also be noted here that the second predetermined time corresponding to the first filling control valve 342 or the second filling control valve 352 can be the same or different, and is specifically determined by the capacity of the corresponding preparation bag 500 that is not filled with cell fluid and the flow rate of the cell fluid in the main line 270 when emptying.

[0066] In an embodiment of the present invention, Figure 2 The schematic diagram of the assembly relationship structure of the first pump body, the second pump body and the third sub-assembly panel provided in the embodiment of the present invention is illustrated as follows: Figure 1 and Figure 2 As shown, the flow detection component includes a drip bucket 230 , which is connected in series to the main line 270 and is located between the second pump body 210 and the first control valve 120 .

[0067] The flow detection component also includes a drip bucket rotating member 231. The drip bucket rotating member 231 is a motor or a combination of a motor and a reducer. Preferably, the drip bucket rotating member 231 is a servo motor to precisely control the motor's rotation angle. The drip bucket rotating member 231 is connected to the drip bucket 230. Specifically, the motor's rotating shaft is provided with a fixing member, and the drip bucket 230 is fixed to the fixing member. The drip bucket rotating member 231 is connected to a controller and is used to drive the drip bucket 230 to rotate.

[0068] When second control valve 130 opens, main line 270 begins to drain, and the controller simultaneously begins timing. When second control valve 130 opens for a third predetermined time, the controller controls the rotation of drip bucket rotator 231, which drives drip bucket 230 to rotate 180° at a first predetermined speed. The rotation speed of drip bucket 230 is determined by the controller. By controlling the rotation of drip bucket 230, all cell fluid remaining in drip bucket 230 can flow toward second pump body 210, facilitating the emptying of cell fluid remaining in drip bucket 230 and thereby reducing cell fluid waste in main line 270. In this embodiment, the third predetermined time is the time it takes for air to empty the cell fluid volume in main line 270 between the liquid inlet of drip bucket 230 and the second connection port of first control valve 120, as well as one-third of the cell fluid volume of drip bucket 230.

[0069] It should be noted that since the flow rate of the cell fluid in the pipeline is constant and the volume of the cell fluid in the main pipeline 270 is also known, the third predetermined time can be calculated based on the flow rate and the volume when the drip bucket 230 is emptied of one-third of the cell fluid. The third predetermined time can be set according to actual usage requirements. The first predetermined speed is specifically determined based on the type of cell fluid and is preferably slow to reduce shear force on the cells and avoid damaging cell activity.

[0070] In an embodiment of the present invention, Figure 3 The schematic diagram of the assembly relationship between the quantitative tube rotating member, the second bubble sensor, the third bubble sensor and the second filling panel provided in the embodiment of the present invention is illustrated as follows: Figures 1 to 3 As shown, the flow detection component also includes a first bubble sensor 240 and a second bubble sensor 250. The first bubble sensor 240 is disposed in the first pipeline 160 and is located between the first pump body 110 and the mixing bag 150. In this embodiment, the first bubble sensor 240 is clamped to the first pipeline 160. After entering the first pipeline 160, the liquid in the mixing bag 150 passes through the first bubble sensor 240 and enters the second pipeline 170. The first bubble sensor 240 is used to detect in real time whether cell fluid is flowing through the first pipeline 160. If the first bubble sensor 240 detects that no cell fluid is flowing through the first pipeline 160, the liquid in the mixing bag 150 has been completely transferred to the second pipeline 170.

[0071] The second bubble sensor 250 is disposed in the main line 270 and is located between the flow detection element 220 and the second pump body 210. In this embodiment, the second bubble sensor 250 is clamped to the main line 270 and is used to detect in real time whether cell fluid is flowing through the main line 270 between the second pump body 210 and the liquid inlet of the flow detection element 220. If the second bubble sensor 250 detects that no cell fluid is flowing through the main line 270, the cell fluid in the main line 270 between the second bubble sensor 250 and the first control valve 120 has been drained. The first bubble sensor 240 and the second bubble sensor 250 are both electrically connected to the controller, enabling the controller to obtain detection information from the first bubble sensor 240 and the second bubble sensor 250 and record the time when the first bubble sensor 240 and the second bubble sensor 250 begin to detect that no cell fluid is flowing.

[0072] In an embodiment of the present invention, the flow detection component 220 is a dosing tube, which is used to detect the flow rate of the cell fluid within the main line 270. The flow detection component also includes a dosing tube rotating member 221, which is connected to the dosing tube. The dosing tube rotating member 221 is a motor or a combination of a motor and a reducer. Preferably, the dosing tube rotating member 221 is a servo motor. The dosing tube is connected to the rotating shaft of the motor, and the dosing tube rotating member 221 is electrically connected to a controller and is used to drive the dosing tube to rotate.

[0073] When the second bubble sensor 250 detects that no cell fluid flows through the main line 270, that is, most of the cell fluid in the main line 270 has been transferred to the branch line, the quantitative tube rotating member 221 drives the quantitative tube to rotate, so that all the cell fluid remaining in the quantitative tube can flow to the branch line, making it easier to empty the cell fluid remaining in the quantitative tube, thereby further reducing the waste of cell fluid in the main line 270.

[0074] In an embodiment of the present invention, the flow detection component also includes a third bubble sensor 260, which is electrically connected to the controller. The third bubble sensor 260 is arranged in the main line 270 and is located between the flow detection component 220 and the branch line. In this embodiment, the third bubble sensor 260 is clamped in the main line 270. The third bubble sensor 260 is used to detect in real time whether there is cell fluid flowing in the main line 270 between the liquid outlet of the flow detection component 220 and the branch line.

[0075] When the second bubble sensor 250 detects that no cell fluid begins to flow through the main line 270, and cell fluid still flows through the third bubble sensor 260, at this time, air has not yet entered the quantitative tube, or a small amount of air has entered the quantitative tube. The quantitative tube rotating member 221 drives the quantitative tube to rotate, so that the packaging control device can empty the cell fluid remaining in the quantitative tube, further reducing the waste of cell fluid in the main line 270.

[0076] In another embodiment of the present invention, when the drip bucket rotating member 231 drives the drip bucket 230 to rotate at a first predetermined speed, the controller simultaneously controls the quantitative tube rotating member 221 to drive the quantitative tube to rotate at a second predetermined speed, so that the cell fluid remaining in the drip bucket 230 will not remain in the quantitative tube again, thereby shortening the time for air to empty the main line 270 and improving the emptying efficiency of the packaging control device.

[0077] In an embodiment of the present invention, the dispensing assembly further includes a fourth bubble sensor 320, which is electrically connected to the controller and disposed at one end of the branch line proximate to the main line 270. In this embodiment, the fourth bubble sensor 320 is clamped to the branch line proximate to the main line 270. The fourth bubble sensor 320 is configured to detect in real time whether cell fluid is flowing through the branch line. When the fourth bubble sensor 320 detects that no cell fluid is flowing through the branch line, it indicates that the main line 270 in front of the fourth bubble sensor 320 has been completely drained of cell fluid.

[0078] In an embodiment of the present invention, the dispensing component further includes an air pressure sensor 310, which is electrically connected to the controller and disposed at the end of the branch line away from the main line 270. The air pressure sensor 310 is used to monitor the liquid pressure in the branch line in real time as the cell fluid is dispensed into the preparation bag 500. If the pressure detected by the air pressure sensor 310 exceeds a preset pressure value, the air pressure sensor 310 generates a feedback signal, causing the controller to reverse the rotation of the second pump body 210, thereby reducing the flow rate of the main line 270 and the liquid pressure in the main line 270 and the branch line.

[0079] The present invention further provides a subpackaging device, comprising a body and the subpackaging control device described in any one of the above embodiments, wherein the subpackaging control device is arranged in the body.

[0080] The body is provided with a first filling panel 410, a second filling panel 420, and a third filling panel 430. The first filling panel 410 is provided on the upper portion of the second filling panel 420, and the second filling panel 420 is provided on the upper portion of the third filling panel 430. The lower edges of the first filling panel 410 and the second filling panel 420 are both provided with a collecting groove. The bottom of the body is provided with a drainage groove, which is connected to the collecting groove. The first pump body 110, the second pump body 210, the first bubble sensor 240, the first control valve 120 and the second control valve 130 are provided on the third filling panel 430. The second filling component, the quantitative tube rotating member 221, the quantitative tube, the second bubble sensor 250 and the third bubble sensor 260 are provided on the second filling panel 420. The first filling component is provided on the first filling panel 410.

[0081] The following combination Figure 5 The present invention describes a packaging method. Figure 5 The flowchart of the packaging control method provided by the embodiment of the present invention is illustrated as follows. Figure 5 As shown, the packaging method provided by the present invention is based on the packaging control device described in any one of the above embodiments, and the packaging method includes:

[0082] Step S10 , controlling the first pump body 110 and the second pump body 210 to operate so that the cell fluid fills the main line 270 and the branch line.

[0083] The first pump body 110 is controlled by the controller to operate, driving the liquid in the sample bag 140 into the first pipeline 160. The liquid in the mixing bag 150 enters the first pipeline 160 under the action of its own gravity. The liquid in the sample bag 140 and the liquid in the mixing bag 150 are mixed in the second pipeline 170 to obtain cell fluid. The second pump body 210 is controlled by the controller to operate, driving the cell fluid to fill the main pipeline 270 and the branch pipeline.

[0084] Step S20 , sequentially controlling the multiple sub-packaging control valves of each sub-packaging component to open in a predetermined order for a first predetermined time, so that the remaining volume of the preparation bag 500 corresponding to each sub-packaging component is equal to the cell fluid volume in the corresponding pipeline.

[0085] When the cell fluid fills the main line 270 and the branch lines, the controller controls the multiple sub-packaging control valves of each sub-packaging component to open in sequence for a first predetermined time in a predetermined order, so that the cell fluid is dispensed into the preparation bag 500 corresponding to each sub-packaging control valve in the predetermined order. After the sub-packaging is completed, each sub-packaging component has at least one preparation bag 500 that has not been dispensed with cell fluid or has been dispensed with a small amount of cell fluid. The capacity of the preparation bag 500 that has not been dispensed with cell fluid is referred to as the residual capacity. The residual capacity of the preparation bag 500 corresponding to each sub-packaging component is equal to the cell fluid capacity in the corresponding pipeline. In this embodiment, the cell fluid capacity is the amount of cell fluid stored in the corresponding pipeline.

[0086] In step S30 , the first control valve 120 is controlled to be closed and the second control valve 130 is controlled to be open.

[0087] After completing the packaging of the preparation bags 500 corresponding to some of the packaging control valves, the controller controls the second pump body 210 to start by closing the first control valve 120 and opening the second control valve 130. Driven by the second pump body 210, air enters the main line 270 from the air inlet line 180 and pushes the residual cell fluid in the main line 270 to flow into the branch line, and the main line 270 begins to be emptied.

[0088] Step S40 , sequentially controlling the sub-packaging control valves corresponding to each group of preparation bags 500 that are not filled with cell fluid to open for a second predetermined time, so that the cell fluid in the corresponding pipeline is loaded into the corresponding preparation bag 500 that is not filled with cell fluid.

[0089] In one embodiment of the present invention, step S20 includes the following steps:

[0090] In step S21, the multiple first sub-packaging control valves 342 of the first sub-packaging component are controlled to open in sequence for a first predetermined time in a predetermined order so that the remaining volume of the preparation bag 500 corresponding to the first sub-packaging component is equal to the sum of the cell fluid volume in the main line 270 and the first branch line 341.

[0091] When the cell sap fills the main line 270 and the first branch line 341, the controller controls the multiple first sub-packaging control valves 342 of the first sub-packaging component to open sequentially for a first predetermined time in a predetermined order, and the cell sap is dispensed into the preparation bag 500 corresponding to each first sub-packaging control valve 342 in the predetermined order. The one or more first sub-packaging control valves 342 located at the end of the first branch line 341 away from the main line 270 are closed, and the preparation bags 500 corresponding to these first sub-packaging control valves 342 are not involved in the sub-packaging. The remaining capacity of the unfilled preparation bags 500 in the first sub-packaging component is equal to the sum of the undispensed cell sap volumes in the main line 270 and the first branch line 341. When a first sub-packaging control valve 342 on the first branch pipeline 341 is closed, the remaining capacity of the preparation bag 500 is the remaining capacity of the preparation bag 500 corresponding to the first sub-packaging component; when multiple first sub-packaging control valves 342 on the first branch pipeline 341 are closed, the remaining capacity of the preparation bag 500 is the remaining capacity of multiple preparation bags 500 corresponding to the first sub-packaging component.

[0092] In step S22 , the second dispensing control valves 352 of the second dispensing component are controlled to open in sequence for a first predetermined time in a predetermined order so that the remaining volume of the preparation bag 500 corresponding to the second dispensing component is equal to the cell fluid volume in the second branch line 351 .

[0093] After the first branch line 341 completes the filling of the preparation bag 500 corresponding to the first branch line 341, the controller controls the multiple second filling control valves 352 of the second filling component to open sequentially for a first predetermined time in a predetermined order, and the cell fluid is then filled into the preparation bag 500 corresponding to each second filling control valve 352 in the predetermined order. The one or more second filling control valves 352 located at the end of the second branch line 351 remote from the main line 270 are closed, and at least one preparation bag 500 corresponding to the second branch line 351 is not filled. The remaining capacity of the unfilled preparation bag 500 in the second filling component is equal to the unfilled cell fluid volume in the second branch line 351.

[0094] In an embodiment of the present invention, step S40 includes the following steps:

[0095] In step S41 , the first filling control valves 342 corresponding to the first group of preparation bags 500 that are not filled with cell fluid are controlled to open in sequence for a second predetermined time, so that the cell fluid in the main line 270 and the first branch line 341 is filled into the first group of preparation bags 500 that are not filled with cell fluid.

[0096] After completing the subpackaging of most of the preparation bags 500, the controller controls the first subpackaging control valves 342 corresponding to the first group of preparation bags 500 that are not filled with cell fluid to open in sequence for the second predetermined time, so that the cell fluid remaining in the main line 270 and the first branch line 341 is subpacked into the first group of preparation bags 500 that are not filled with cell fluid, completing the subpackaging of the residual cell fluid in the main line 270 and the first branch line 341, and reducing the waste of cell fluid in the main line 270 and the first branch line 341.

[0097] In step S42 , the second filling control valves 352 corresponding to the second group of preparation bags 500 that are not fully filled with cell fluid are controlled to open sequentially for a second predetermined time, so that the cell fluid in the second branch pipe 351 is filled into the second group of preparation bags 500 that are not fully filled with cell fluid.

[0098] After completing the subpackaging of the residual cell fluid in the main pipeline 270 and the first branch pipeline 341, the controller controls the second subpackaging control valves 352 corresponding to the second group of preparation bags 500 that are not filled with cell fluid to open in sequence for a second predetermined time, so that the residual cell fluid in the second branch pipeline 351 is subpacked into the second group of preparation bags 500 that are not filled with cell fluid, completing the subpackaging of the residual cell fluid in the second branch pipeline 351 and reducing the waste of cell fluid in the second branch pipeline 351.

[0099] In an embodiment of the present invention, after executing step S30, the following steps are further included:

[0100] In step S43 , after the second control valve 130 is opened for a third predetermined time, the drip bucket rotating member 231 is controlled to drive the drip bucket 230 to rotate at a first predetermined speed, so that all the cell fluid in the drip bucket 230 flows to the second pump body 210 .

[0101] When the second control valve 130 is opened, the main line 270 begins to be emptied and the controller starts timing. When the opening time of the second control valve 130 reaches the third predetermined time, the controller controls the rotation of the drip bucket 231, and the drip bucket 231 drives the drip bucket 230 to rotate 180° at the first predetermined speed. The rotation speed of the drip bucket 230 is determined according to the specific type of cell fluid. The first predetermined speed is preferably slow to reduce the shear force of the cells and avoid damaging the activity of the cells. By controlling the rotation of the drip bucket 230, all the cell fluid remaining in the drip bucket 230 can flow to the second pump body 210, thereby reducing the waste of cell fluid in the main line 270 between the second connection port of the first control valve 120 and the liquid outlet of the drip bucket 230.

[0102] In an embodiment of the present invention, after executing step S43, the following steps are further included:

[0103] When it is determined that no cell fluid begins to flow through the second bubble sensor 250 and cell fluid begins to flow through the third bubble sensor 260, or after a fourth predetermined time has passed since it is determined that no cell fluid begins to flow through the second bubble sensor 250, the quantitative tube rotating member 221 is controlled to drive the quantitative tube to rotate at a second predetermined speed so that all the cell fluid in the quantitative tube enters the branch line.

[0104] When second bubble sensor 250 detects no cell fluid flowing through and third bubble sensor 260 detects cell fluid flowing through, or after a fourth predetermined period of time has passed since cell fluid ceased to flow through second bubble sensor 250, most of the cell fluid in main line 270 before second bubble sensor 250 has been transferred to the branch line, and air has not yet entered the quantitative tube, or a small amount of air has entered the quantitative tube. The controller controls the quantitative tube rotating member 221 to rotate the quantitative tube at a second predetermined speed, which is equal to the first predetermined speed, so that all cell fluid remaining in the quantitative tube can enter the first branch line 341, thereby reducing the waste of cell fluid in the quantitative tube.

[0105] It should be noted here that since the flow rate in the pipeline is known, the cell fluid volume of the main pipeline 270 between the second bubble sensor 250 and the quantitative tube is also known, so the fourth predetermined time can be calculated based on the above cell fluid volume and flow rate.

[0106] The packaging method provided by the present invention can effectively improve the packaging accuracy and packaging efficiency of cell packaging, thereby increasing the survival rate of cells and preventing cell functions from being adversely affected during the packaging process.

[0107] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. A packaging control device, characterized in that: include: The flow detection component comprises a second pump body, a flow detection element and a main pipe, wherein the second pump body is arranged on the main pipe; The flow detection element is connected in series to the main pipeline and is located in the downstream direction of the second pump body; The delivery component includes a first pump body, a first control valve, a second control valve and a pipeline assembly, wherein the pipeline assembly includes a first pipeline, a second pipeline and an air intake pipeline, wherein the first pipeline is respectively connected to the sample bag and the mixing bag, and the first pump body is arranged in the first pipeline; The second pipeline is communicated with the first pipeline and the air intake pipeline respectively, the first control valve is arranged on the second pipeline, and the second control valve is arranged on the air intake pipeline; The sub-packaging component includes a plurality of sub-packaging control valves, branch lines connected to the main line, and a plurality of sub-packaging pipes connected to the branch lines, wherein the plurality of sub-packaging control valves are provided in a one-to-one correspondence with the plurality of sub-packaging pipes, and the plurality of sub-packaging pipes are connected to the preparation bags in a one-to-one correspondence; a controller electrically connected to the first pump body, the first control valve, the second control valve, the sub-packaging control valve, the second pump body, and the flow detection element respectively; The flow detection component also includes: a drip bucket, connected in series to the main pipe and located between the second pump body and the first control valve; A drip bucket rotating member is connected to the drip bucket, the drip bucket rotating member is electrically connected to the controller, and the drip bucket rotating member is used to drive the drip bucket to rotate; a first bubble sensor and a second bubble sensor, the first bubble sensor is arranged in the first pipeline, and is located between the first pump body and the mixing bag; the second bubble sensor is arranged in the main pipeline, and is located between the flow detection member and the second pump body; the first bubble sensor and the second bubble sensor are both electrically connected to the controller; a third bubble sensor is electrically connected to the controller, the third bubble sensor is arranged in the main pipeline, and is located between the flow detection member and the branch pipeline; the flow detection member is a quantitative tube, and the flow detection component also includes a quantitative tube rotating member, connected to the quantitative tube, the quantitative tube rotating member is electrically connected to the controller, and the quantitative tube rotating member is used to drive the quantitative tube to rotate.

2. The packaging control device according to claim 1, characterized in that: The subpackaging components also include: a fourth bubble sensor, electrically connected to the controller, and disposed at one end of the branch pipe close to the main pipe; An air pressure sensor is electrically connected to the controller, and the air pressure sensor is arranged at one end of the branch pipe away from the main pipe.

3. The packaging control device according to claim 1, characterized in that: The subpackaging control device includes a first subpackaging component and a second subpackaging component, the first subpackaging component includes a first branch pipeline, multiple first subpackaging control valves and multiple first subpackaging pipes connected to the first branch pipeline, and the multiple first subpackaging control valves are arranged in a one-to-one correspondence with the multiple first subpackaging pipes; the second subpackaging component includes a second branch pipeline, multiple second subpackaging control valves and multiple second subpackaging pipes connected to the second branch pipeline, and the multiple second subpackaging control valves are arranged in a one-to-one correspondence with the multiple second subpackaging pipes.

4. A packaging device, characterized in that: The invention comprises a machine body and the subpackaging control device according to any one of claims 1 to 3, wherein the subpackaging control device is arranged in the machine body.

5. A subpackaging method, the subpackaging method being based on the subpackaging control device according to any one of claims 1 to 3, characterized in that: The packaging method comprises: Step S10, controlling the first pump body and the second pump body to operate so that the cell fluid fills the main pipe and the branch pipe; Step S20, sequentially controlling the multiple filling control valves of each filling component to open in a predetermined order for a first predetermined time, so that the remaining volume of the preparation bag corresponding to each filling component is equal to the cell fluid volume in the corresponding pipeline; Step S30, controlling the first control valve to close and the second control valve to open; Step S40 , sequentially controlling the sub-packaging control valves corresponding to each group of preparation bags that are not filled with cell fluid to open for a second predetermined time, so that the cell fluid in the corresponding pipeline is filled into the corresponding preparation bag that is not filled with cell fluid.

6. The packaging method according to claim 5, wherein: After executing step S30, the following steps are also included: Step S43: After the second control valve is opened for a third predetermined time, the drip bucket rotating member is controlled to drive the drip bucket to rotate at a first predetermined speed, so that all the cell fluid in the drip bucket flows to the second pump body.

7. The packaging method according to claim 6, wherein: After executing step S43, the following steps are also included: When it is determined that no cell fluid begins to flow through the second bubble sensor and cell fluid begins to flow through the third bubble sensor, or after a fourth predetermined time has passed after it is determined that no cell fluid begins to flow through the second bubble sensor, the quantitative tube rotating member is controlled to drive the quantitative tube to rotate at a second predetermined speed so that all the cell fluid in the quantitative tube enters the branch line.

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