A circulating tumor cell reverse enrichment device

Abstract

The present invention relates to the field of medical device technology, and specifically to a circulating tumor cell reverse enrichment device, the device comprising an operating table, a frame body arranged on the operating table, a mechanical arm installed on the frame body, and a sample tank, a reagent tank, a reaction tank and a magnetic separation device arranged on the operating table in sequence. The mechanical arm is used to transfer the sample to be tested between the sample tank, the reagent tank, the reaction tank and the magnetic separation device, and by using the immunomagnetic bead reverse enrichment technology, various types of more diverse CTCs are retained to the maximum extent, including those CTCs that may lose traditional markers due to phenotypic conversion and those with low EpCAM expression, thereby improving the efficiency and accuracy of CTC capture, providing a more powerful tool for early diagnosis, treatment monitoring and prognosis evaluation of cancer, and truly meeting clinical needs.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and in particular to a circulating tumor cell reverse enrichment device. Background Art

[0002] Tumor metastasis is the main cause of death in cancer patients. Malignant tumor cells can spread through the blood to other organs in the body. How to detect cancer earlier and more efficiently and strike it accurately is one of the most concerned topics in the world.

[0003] Tumor cells invade the surrounding tissues of primary tumor cells, enter the blood and lymphatic system, form circulating tumor cells CTC, and are transported to distal tissues, then infiltrate, adapt to the new microenvironment, and eventually "seed", "proliferate", "colonize", and form metastatic lesions. Therefore, early detection of CTCs in the blood plays an important guiding role in patient prognosis, efficacy evaluation, and personalized treatment. The detection and analysis of circulating tumor cells (CTCs) are of great significance for the early detection of cancer, evaluation of treatment effects, and prognosis of the disease. In recent years, CTC enrichment and detection technology has indeed made significant progress, including the transition from forward enrichment technology to reverse enrichment technology. The following are some major technical introductions:

[0004] Forward enrichment technology: This type of technology mainly relies on the recognition of specific markers on the surface of CTCs, such as EpCAM (epithelial cell adhesion molecule), and captures CTCs using anti-EpCAM antibodies. A well-known example of the forward enrichment method is Veridex's CellSearch system, which is the first and so far the only CTC detection system approved by the FDA for clinical use. However, one limitation of the forward enrichment technology is that, mainly because a large proportion of tumor cells with tumor stemness and metastatic potential either have epithelial-mesenchymal transition (EMT) or disguise surface markers (low or lost EpCAM expression), leading to immune escape, etc., the positive rate of CTCs that undergo phenotypic transformation during cancer progression is extremely low when using forward enrichment technology, which limits its clinical application. Therefore, Johnson & Johnson has abandoned the further promotion of the CellSearch system.

[0005] Reverse enrichment technology: In contrast to forward enrichment, reverse enrichment technology does not directly target CTC markers, but enriches CTCs by removing non-tumor cells (such as red blood cells and white blood cells) from the blood. This method usually involves differences in physical properties (such as size, density, charge, etc.) to distinguish CTCs from other blood cells. One advantage of reverse enrichment technology is that it does not rely on surface markers of CTCs, so it can capture more diverse CTCs, including those that may lose traditional markers due to phenotypic conversion. CTC enrichment based on microfluidics technology: This type of technology uses microfluidics principles to separate CTCs, and can separate cells based on cell size, morphology or softness. Microfluidic devices are usually highly integrated and automated, capable of handling trace samples, and excel in cell capture efficiency and purity.

[0006] CTC enrichment based on immunomagnetic separation: This technology combines the principles of immunological recognition and magnetic separation, and can be either positive or negative enrichment. By using magnetic microbeads coated with specific antibodies, specific cells can be efficiently captured or removed. This method is highly flexible and can be used for positive enrichment of specific cell surface markers or for negative enrichment by removing specific non-target cells.

[0007] Microcolumn and chip-based technologies: Using microcolumns or microstructures on chips to achieve CTC enrichment and separation, this type of technology relies on precise engineering design and can capture and analyze CTCs under high-throughput and high-efficiency conditions. With the development of technology, CTC enrichment and detection methods are constantly improving and innovating, aiming to improve the efficiency and accuracy of CTC capture, and provide more powerful tools for early diagnosis, treatment monitoring, and prognosis evaluation of cancer.

[0008] Existing technologies are mainly forward enrichment technologies, which rely on the recognition of specific markers on the surface of CTCs, such as EpCAM (epithelial cell adhesion molecule), and capture CTCs using anti-EpCAM antibodies. A well-known example of this technology is Veridex's CellSearch system, which is the first and so far the only FDA-approved CTC detection system for clinical use. However, one limitation of this enrichment technology is that it may miss CTCs with low EpCAM expression or phenotypic conversion during cancer progression, so the efficiency and accuracy of CTC capture are not high.

[0009] Existing patents provide some solutions, such as patent EP3929591A1, which provides a dark box detection system for magnetic particle chemiluminescence immunoassay, including a substrate, a detection dark box and a reaction cup holder, wherein a dark chamber with an opening on one side is provided inside the detection dark box; the reaction cup holder has a cup placement slot for placing the reaction cup, and can slide relative to the detection dark box to open or close the inlet and outlet, and the reaction cup holder has a detection and cup placement position within its sliding stroke, when the reaction cup holder is in the detection position, the cup placement slot is in the dark room, and the inlet and outlet are closed, when the reaction cup holder is in the cup placement position, the cup placement slot is outside the dark room, and the inlet and outlet are open; a driving mechanism is used to drive the reaction cup holder to slide; a detection mechanism is used to detect the luminescence amount of the liquid in the reaction cup in the dark room; a transfer device is used to put the reaction cup into the cup placement slot or to remove the reaction cup from the cup placement slot. The main method is to adopt an open-door detection dark box structure that is relatively independent of the gripper to avoid mutual interference between substrate filling and the gripper negative pressure suction nozzle, and to improve the gripper action and the reliability of the detection results. However, the invention still has a lot of room for improvement in terms of achieving improved efficiency and accuracy of CTC capture. Summary of the invention

[0010] In order to improve the efficiency and accuracy of CTC capture, avoid tilting of the reagent tube, improve the efficiency of separation detection, and reduce the possibility of damage to the reagent tube during the detection process.

[0011] The present invention provides a circulating tumor cell reverse enrichment device, which comprises an operating table, a frame body is arranged on the operating table, a mechanical arm is installed on the frame body, a sample slot, a reagent slot, a reaction slot and a magnetic separation device are arranged on the operating table in sequence, the mechanical arm is used to transfer the sample to be tested between the sample slot, the reagent slot, the reaction slot and the magnetic separation device, and a sample adding needle for sucking the sample is installed on the mechanical arm, and the sample adding needle realizes the reverse enrichment operation of the sample in the magnetic separation device.

[0012] Furthermore, the circulating tumor cell reverse enrichment device involved in the present invention, wherein the specific transfer enrichment CTC capture method is: 7.5mL of blood is transferred to a conical tube and centrifuged. After centrifugation, three layers of red blood cells, white blood cells and platelets, and plasma are formed. The upper layer of plasma is aspirated and removed. Then, red blood cell lysis solution is added and mixed to lyse the red blood cells. Centrifugation is then performed to collect the white blood cell layer at the bottom.

[0013] The buffy coat is manipulated to remove most of the white blood cells.

[0014] There are many methods for leukocyte removal, such as inverted centrifugation, accelerated sedimentation, special filtration, blood cell separator preparation, etc. The most commonly used is the special filtration method, which uses a dedicated leukocyte filter to remove leukocytes from whole blood.

[0015] The sample from which most of the leukocytes have been removed is subjected to the circulating tumor cell reverse enrichment device of the present invention, and the remaining leukocytes are enriched with CD45 magnetic beads, and the remaining substances are the desired target CTCs.

[0016] The sample needle on the robotic arm draws the sample from the sample cup and adds it to the reaction cup. The robotic arm then draws anti-CD45 antibody immunomagnetic beads from the reagent tank and adds them to the reaction cup. Mix and incubate. Then the reaction cup is placed in the magnetic separation device for magnetic separation. The robotic arm controls the sample needle to draw all the liquid that is not attracted by the magnetic field and puts it into another new sample cup. This completes the entire CTC extraction process. Because the reverse enrichment technology of immunomagnetic beads is used, it can retain a variety of more diverse CTCs to the maximum extent, including those that may lose traditional markers due to phenotypic conversion and CTCs with low EpCAM expression, improve the efficiency and accuracy of CTC capture, and provide a more powerful tool for early diagnosis, treatment monitoring and prognosis evaluation of cancer.

[0017] In the present invention, a slide rail is installed on one side of the mechanical arm, a driving device is installed on one side of the slide rail, and the driving device and the slide rail are respectively installed on two sides of the frame.

[0018] Furthermore, the driving device drives the mechanical arm to reciprocate on the slide rail to sample and transport the samples.

[0019] In the present invention, the robot arm comprises a first swing arm, the end of the first swing arm is connected to a base plate, a pair of second swing arms which can rotate relatively are arranged on the base plate, and auxiliary parts are arranged relatively on the side wall surface of the end of the second swing arm.

[0020] Furthermore, the auxiliary parts arranged on the side of the second swing arm are in the shape of convex dots and are arranged in an array on the side wall surface of the second swing arm near the end, which can enhance the clamping effect of the robot arm on the reagent. At the same time, since the auxiliary parts are elastic parts, they can also have better clamping adaptability to reagent tubes of different shapes and sizes, and can also avoid the problem of the reagent tube breaking due to excessive clamping force of the second swing arm.

[0021] In the present invention, a first plate body extending downward is arranged at one end of the base plate, an alignment base body is installed on the first plate body, the alignment base body is arranged between the second swing arms, and a limit block is arranged above the alignment base body.

[0022] Furthermore, an alignment base and a buffer assembly are provided to correct the symmetrical effect of the second swing arm after the relative swing and reset, that is, the buffer assembly fixedly connected to the side of the alignment base is parallel, and when the second swing arm is opened and does not contact the buffer assembly and then returns to its original state, it can ensure that the second swing arms on both sides are symmetrically arranged, thereby ensuring that the robotic arm can work efficiently and the sampling work can be carried out efficiently, thereby avoiding changes in the clamping contact surface and angle between the auxiliary part under the second swing arm and the reagent tube. If the second swing arm is not symmetrically arranged, it is easy to cause the auxiliary part on one side of the reagent tube to have a larger contact surface and a smaller contact surface on the other side, which will result in the occurrence of a clamping tilt problem.

[0023] In the present invention, a buffer component is fixedly connected to the side of the alignment base, and the buffer component has a limiting effect on the swing angle of the second swing arm.

[0024] Furthermore, the buffer assembly is arranged on the side of the alignment base, which can support the side of the second swing arm, thereby limiting the inward clamping range of the second swing arm, thereby avoiding the problem of the reagent tube being broken due to the second swing arm clamping the reagent tube excessively inward.

[0025] In the present invention, the buffer assembly comprises a connecting plate body connected to the alignment base, and one end of the connecting plate body is provided with at least two elliptical telescopic sleeves.

[0026] In the present invention, the telescopic sleeves are connected through a first rod body, a first spring is sleeved on the first rod body, and two ends of the first spring are connected to the telescopic sleeve.

[0027] Furthermore, the first rod body arranged between the telescopic sleeves is a telescopic structure. After the telescopic sleeves are compressed, the first spring sleeved on the outside of the first rod body is compressed together with the first rod body. Since the telescopic sleeve is compressed, the second swing arm must swing inward to clamp the reagent tube. Therefore, the structure formed by the first spring and the telescopic sleeve is conducive to alleviating the shaking of the moving reagent tube, so as to avoid the vibration of the slider on the robot arm being transmitted to the reagent tube without buffering during the movement, causing shaking of the sample in the reagent tube and possibly spilling the internal sample. For example, for the reagent tube that has been centrifuged and the medium inside has been stratified, if the shaking amplitude of the clamped second swing arm is too large, the stratification of the originally separated sample may not be obvious, affecting subsequent operations. In addition, the buffer assembly and the auxiliary parts improve the centering accuracy of the second swing arm and the reagent tube clamping. In this way, after the clamped reagent tube is moved to the next workstation, it can be accurately placed in the coordinate point, and it is not easy for the reagent tube to tilt during the placement process. The tilted reagent tube is prone to internal material flowing out of the wall of the reagent tube or other problems when it is placed in the coordinate point.

[0028] In the present invention, a power source is arranged on the side of the base plate opposite to the second swing arm, and the power source controls the rotation of the second swing arm.

[0029] In the present invention, a slider is installed on the slide rail, and the slider is fixedly connected to the rear end of the robot arm. The slider is a square base, and a through groove body adapted to the slide rail is opened in the middle. A rotating wheel is arranged inside the slider, and the rotating wheel is adapted to the surface of the slide rail. A plug-in block is also installed on the side of the slider, and the plug-in block includes a frame with a central groove, and a plurality of blades are arranged in an array in the frame, and the blades are arranged relative to the surface of the slide rail.

[0030] Furthermore, the driving mode of the driving device adopted on one side of the slider is motor driven, and the rotating wheel provided can improve the sliding smoothness and stability. At the same time, the plug-in block installed on the side of the slider can also be used to limit the moving stroke, that is, the mechanical arm is controlled within the area of ​​the sample tank, the reagent tank, the reaction tank and the magnetic separation device, reducing the possibility of the mechanical arm moving out of the area, which has a limiting effect on the mechanical arm, and at the same time, the blades arranged in the internal array of the plug-in block have a certain interval between them, which can have the effect of relieving collision buffering. Due to the instant acceleration brought by the motor drive, it is easy for the mechanical arm to vibrate to a certain extent when starting, so the plug-in block can use the small rubber pads arranged on the surface of the blade to absorb the vibration when moving relative to the slide rail, and can also use the blade to collect the lubricating oil on the slide rail, and evenly disperse the lubricating oil on the slide rail through the blade. At the same time, during the displacement process of the slider, the rubber pads arranged on the surface of the blade can also have a certain cleaning effect on the slide rail, and the collection of lubricating oil can prevent the lubricating oil from forming a small part and dripping into the reagent below.

[0031] Compared with the prior art, the significant technical progress of the present invention lies in: by using the immunomagnetic bead reverse enrichment technology, various types of more diverse CTCs are retained to the maximum extent, including those CTCs that may lose traditional markers due to phenotypic conversion and those with low EpCAM expression, thereby improving the efficiency and accuracy of CTC capture, providing a more powerful tool for early diagnosis, treatment monitoring and prognosis evaluation of cancer, and truly meeting clinical needs; by setting an alignment base and a buffer component to correct the symmetry effect after the relative swinging and resetting of the second swing arm, that is, the buffer component fixedly connected to the side of the alignment base is parallel, and when the second swing arm is opened without contacting the buffer component and then the second swing arm returns to its original state, it can ensure that the second swing arms on both sides are symmetrically arranged, thereby ensuring that the robotic arm can work efficiently and the sampling work is carried out efficiently, thereby avoiding changes in the clamping contact surface and angle between the auxiliary part under the second swing arm and the reagent tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 It is a schematic structural diagram of a circulating tumor cell reverse enrichment device involved in the present invention;

[0033] Figure 2This is a schematic diagram of the capture process of transfer-enriched CTCs involved in the present invention;

[0034] Figure 3 This is a schematic diagram of the connection structure of the mechanical arm involved in the present invention;

[0035] Figure 4 It is a schematic diagram of another viewing angle of the mechanical arm connection structure involved in the present invention;

[0036] Figure 5 This is a schematic diagram of the structure of the buffer assembly involved in the present invention;

[0037] Figure 6 for Figure 3 The enlarged structural diagram at A in the middle;

[0038] Figure 7 for Figure 4 Enlarged structural diagram at B in the middle.

[0039] Explanation of reference numerals: 11-operating table; 12-frame; 13-sample slot; 14-reagent slot; 15-reaction slot; 16-magnetic separation device; 2-mechanical arm; 21-first swing arm; 22-base plate; 221-first plate body; 23-second swing arm; 24-auxiliary part; 27-buffer assembly; 271-connecting plate body; 272-telescopic sleeve; 273-first rod body; 274-first spring; 3-slide rail; 31-slider; 33-plug-in block; 331-frame body; 332-blade DETAILED DESCRIPTION

[0040] Embodiment 1:

[0041] The present invention provides a circulating tumor cell reverse enrichment device, which includes an operating table 11, a frame 12 is arranged on the operating table 11, a mechanical arm 2 is installed on the frame 12, a sample slot 13, a reagent slot 14, a reaction slot 15 and a magnetic separation device 16 are arranged on the operating table 11 in sequence, the mechanical arm 2 is used to transfer the sample to be tested between the sample slot 13, the reagent slot 14, the reaction slot 15 and the magnetic separation device 16, and a sample adding needle for sucking the sample is installed on the mechanical arm 2, and the sample adding needle realizes the reverse enrichment operation of the sample in the magnetic separation device 16.

[0042] Furthermore, the circulating tumor cell reverse enrichment device involved in the present invention, wherein the specific transfer enrichment CTC capture method is: 7.5mL of blood is transferred to a conical tube and centrifuged. After centrifugation, three layers of red blood cells, white blood cells and platelets, and plasma are formed. The upper layer of plasma is aspirated and removed. Then, red blood cell lysis solution is added and mixed to lyse the red blood cells. Centrifugation is then performed to collect the white blood cell layer at the bottom.

[0043] The buffy coat is manipulated to remove most of the white blood cells.

[0044] There are many methods for leukocyte removal, such as inverted centrifugation, accelerated sedimentation, special filtration, blood cell separator preparation, etc. The most commonly used is the special filtration method, which uses a dedicated leukocyte filter to remove leukocytes from whole blood.

[0045] The sample from which most of the leukocytes have been removed is subjected to the circulating tumor cell reverse enrichment device of the present invention, and the remaining leukocytes are enriched with CD45 magnetic beads, and the remaining substances are the desired target CTCs.

[0046] The sample needle on the robot arm 2 draws the sample from the sample cup and adds it to the reaction cup. The robot arm 2 then draws the anti-CD45 antibody immunomagnetic beads from the reagent tank 14 and adds them to the reaction cup. The mixture is mixed and incubated. The reaction cup is then placed in the magnetic separation device 16 for magnetic separation. The robot arm 2 controls the sample needle to draw all the liquid that is not attracted by the magnetic field and puts it into another new sample cup. Thus, the entire CTC extraction process is completed. Due to the reverse enrichment technology of immunomagnetic beads, various types of more diverse CTCs can be retained to the maximum extent, including those that may lose traditional markers due to phenotypic conversion and CTCs with low EpCAM expression, thereby improving the efficiency and accuracy of CTC capture and providing a more powerful tool for early diagnosis, treatment monitoring and prognosis evaluation of cancer.

[0047] Embodiment 2:

[0048] In the present invention, a slide rail 3 is installed on one side of the mechanical arm 2 , a driving device 4 is installed on one side of the slide rail 3 , and the driving device 4 and the slide rail 3 are installed on two sides of the frame 12 respectively.

[0049] Furthermore, the driving device 4 drives the robot arm 2 to reciprocate on the slide rail 3 to sample and transport the samples.

[0050] In the present invention, the robot arm 2 includes a first swing arm 21, the end of which is connected to a base plate 22, a pair of second swing arms 23 that can rotate relatively are arranged on the base plate 22, and auxiliary parts 24 are arranged relatively on the side wall surface of the end of the second swing arm 23.

[0051] Furthermore, the auxiliary part 24 arranged on the side of the second swing arm 23 is in the shape of a convex point and is arranged in an array on the side wall surface of the second swing arm 23 near the end, which can enhance the clamping effect of the robot arm 2 on the reagent. At the same time, since the auxiliary part 24 is an elastic component, it can also have better clamping adaptability to reagent tubes of different shapes and sizes, and can also avoid the problem of the reagent tube breaking due to excessive clamping force of the second swing arm 23.

[0052] In the present invention, a first plate body 221 extending downward is provided at one end of the base plate 22, an alignment base is installed on the first plate body 221, the alignment base is arranged between the second swing arms 23, and a limit block is arranged above the alignment base.

[0053] Furthermore, an alignment base and a buffer assembly 27 are provided to correct the symmetrical effect of the second swing arm 23 after relative swinging and resetting, that is, the buffer assembly 27 fixedly connected to the side of the alignment base is parallel, and when the second swing arm 23 is opened and does not contact the buffer assembly 27 and then returns to the original state, it can ensure that the second swing arms 23 on both sides are symmetrically arranged, thereby ensuring that the robot arm 2 can work efficiently and the sampling work can be carried out efficiently, thereby avoiding changes in the clamping contact surface and angle between the auxiliary part 24 under the second swing arm 23 and the reagent tube. If the second swing arm 23 is not symmetrically arranged, it is easy to cause the auxiliary part 24 on one side of the reagent tube to have a larger contact surface and a smaller contact surface on the other side, which will cause the problem of clamping tilt.

[0054] In the present invention, a buffer component 27 is fixedly connected to the side of the alignment base, and the buffer component 27 has a limiting effect on the swing angle of the second swing arm 23 .

[0055] Furthermore, the buffer assembly 27 is arranged on the side of the alignment base, which can support the side of the second swing arm 23, thereby limiting the inward clamping range of the second swing arm 23, thereby avoiding the problem of the reagent tube being broken due to the second swing arm 23 clamping the reagent tube excessively inward.

[0056] In the present invention, the buffer assembly 27 includes a connecting plate body 271 connected to the alignment base, and one end of the connecting plate body 271 is provided with at least two elliptical telescopic sleeves 272 .

[0057] In the present invention, the telescopic sleeves 272 are connected by a first rod 273 , a first spring 274 is sleeved on the first rod 273 , and both ends of the first spring 274 are connected to the telescopic sleeves 272 .

[0058] Furthermore, the first rod 273 disposed between the telescopic sleeves 272 is a telescopic structure. After the telescopic sleeve 272 is compressed, the first spring 274 sleeved on the outside of the first rod 273 is compressed together with the first rod 273. Since the telescopic sleeve 272 is compressed, the second swing arm 23 must swing inward to clamp the reagent tube. Therefore, the structure formed by the first spring 274 and the telescopic sleeve 272 is conducive to alleviating the shaking of the moving reagent tube, thereby preventing the vibration of the slider 31 from being transmitted to the reagent tube without buffering during the movement of the robot arm 2, causing shaking of the sample in the reagent tube, which may cause the internal The problem of sample leakage occurs. For example, the medium inside the reagent tube that has been centrifuged has been stratified. If the second swing arm 23 that is clamped shakes too much, the stratification of the originally separated sample may not be obvious, affecting subsequent operations. In addition, the buffer assembly 27 and the auxiliary component 24 improve the centering accuracy of the second swing arm 23 and the reagent tube clamping. In this way, after the clamped reagent tube is moved to the next workstation, it can be accurately placed in the coordinate point, and it is not easy for the reagent tube to tilt during the placement process. The tilted reagent tube is prone to internal material flowing out of the wall of the reagent tube or other problems when it is placed at the coordinate point.

[0059] In the present invention, a power source is provided on the side of the base plate 22 opposite to the second swing arm 23 , and the power source controls the rotation of the second swing arm 23 .

[0060] Embodiment 3:

[0061] In the present invention, a slider 31 is installed on the slide rail 3, and the slider 31 is fixedly connected to the rear end of the robot arm 2. The slider 31 is a square base, and a through groove body adapted to the slide rail 3 is opened in the middle. A rotating wheel 32 is arranged inside the slider 31, and the rotating wheel 32 is adapted to the surface of the slide rail 3. A plug-in block 33 is also installed on the side of the slider 31. The plug-in block 33 includes a frame 331 with a central groove, and a plurality of blades 332 are arranged in an array in the frame 331, and the blades 332 are arranged relative to the surface of the slide rail 3.

[0062] Furthermore, the driving method of the driving device 4 adopted on one side of the slider 31 is motor-driven, and the rotating wheel 32 provided can improve the sliding smoothness and stability. At the same time, the plug-in block 33 installed on the side of the slider 31 can also be used to limit the moving stroke, that is, the robot arm 2 is controlled within the area of ​​the sample tank 13, the reagent tank 14, the reaction tank 15 and the magnetic separation device 16, reducing the possibility of the robot arm 2 moving out of the area, and having a limiting effect on the robot arm 2. At the same time, the blades 332 arranged in the array inside the plug-in block 33 have a certain interval between them, which can have the effect of easing collision buffering. Due to the instant acceleration brought by the motor drive, the robot arm 2 is likely to vibrate to a certain extent when it starts. Therefore, the plug-in block 33 can use the fine rubber pads arranged on the surface of the blade 332 to absorb the vibration when moving relative to the slide rail 3. At the same time, the blade 332 can also be used to collect the lubricating oil on the slide rail 3, and the lubricating oil on the slide rail 3 is evenly dispersed through the blade 332. At the same time, during the displacement process of the slider 31, the rubber pads arranged on the surface of the blade 332 can also have a certain cleaning effect on the slide rail 3, and the collection of lubricating oil can prevent the lubricating oil from forming a small part and dripping into the reagent below.

[0063] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the attached claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention. Any figure mark in the claims should not be regarded as limiting the claims involved.

Claims

1. A circulating tumor cell reverse enrichment device, comprising an operating table (11), a frame (12) being arranged on the operating table (11), a mechanical arm (2) being installed on the frame (12), a sample tank (13), a reagent tank (14), a reaction tank (15) and a magnetic separation device (16) being arranged on the operating table (11) in sequence, characterized in that: The mechanical arm (2) is used to transfer the sample to be tested between the sample tank (13), the reagent tank (14), the reaction tank (15) and the magnetic separation device (16); a sample adding needle for sucking the sample is installed on the mechanical arm (2); the sample adding needle realizes a reverse enrichment operation on the sample in the magnetic separation device (16); A slide rail (3) is installed on one side of the mechanical arm (2), a drive device (4) is installed on one side of the slide rail (3), and the drive device (4) and the slide rail (3) are respectively installed on two sides of the frame (12); The mechanical arm (2) comprises a first swing arm (21), the end of the first swing arm (21) is connected to a base plate (22), a pair of second swing arms (23) which can rotate relative to each other are arranged on the base plate (22), and auxiliary parts (24) which are arranged opposite to each other are arranged on the side wall surfaces of the ends of the second swing arms (23), and the second swing arms (23) are arranged symmetrically; A first plate body (221) extending downward is provided at one end of the base plate (22), an alignment base body is mounted on the first plate body (221), the alignment base body is arranged between the second swing arms (23), and a limit block is arranged above the alignment base body; A buffer component (27) is fixedly connected to the side surface of the alignment base, and the buffer component (27) has a limiting effect on the swing angle of the second swing arm (23); An alignment base and a buffer assembly (27) are provided to correct the symmetric effect of the second swing arm (23) after relative swinging and resetting, and the buffer assembly (27) fixedly connected to the side of the alignment base is parallel, and the second swing arm (23) is opened without contacting the buffer assembly (27) and then the second swing arm (23) returns to its original state; The buffer assembly (27) comprises a connecting plate body (271) connected to the alignment base, and one end of the connecting plate body (271) is provided with at least two elliptical telescopic sleeves (272); The telescopic sleeves (272) are connected via a first rod body (273); a first spring (274) is sleeved on the first rod body (273); and both ends of the first spring (274) are connected to the telescopic sleeves (272).

2. A circulating tumor cell reverse enrichment device according to claim 1, characterized in that: A power source is provided on a side of the base plate (22) opposite to the second swing arm (23), and the power source controls the rotation of the second swing arm (23).

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